KR20150124643A - Bio cellulose mask pack sheet containing scrub and preparing method thereof - Google Patents

Abstract

The present invention relates to a cellulose mask pack sheet comprising a scrub and, more specifically, to manufacturing of a mask pack sheet having a cleansing and exfoliating effect by containing an oil scrub in a cosmetic mask pack manufactured by putting a microbial strain with a bamboo extract as a culture medium, and fermenting the same to make a bio cellulose sheet, and soaking the bio cellulose sheet in a cosmetic emulsion.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a bio-cellulose mask pack sheet containing a scrubbing agent,

The present invention relates to a mask pack for a bio-cellulose containing a scrubbing agent used for facial skin cosmetics and a method for producing the same, and more particularly, to a mask pack for a cosmetic mask pack using bio-cellulose obtained by fermenting a bamboo extract, The present invention relates to a method of manufacturing a mask pack sheet and a bio-cellulosic mask pack sheet containing a scrubbing agent containing bamboo extract, which is effective for various skin activities effective for skin health in bamboo extract. The present invention also relates to an effective bio-cellulosic mask pack and a method for manufacturing the same, which can improve skin health and contain wrinkles, whitening, moisturizing, exfoliation, and cleansing effects by including a scrubbing agent containing an oil component for a busy modern man.

In general, in the field of cosmetics, a sheet type mask pack is a product that can obtain a moisturizing and cleansing effect by attaching a face-shaped sheet to a face without needing to be hand-worn. It is available in various forms such as 2 sheets of upper and lower sheets, sheets that are tailored to specific areas such as under the eyes, eyes, and mouth, and can be used for the whole body as well as the face and hair according to the purpose of the user.

The sheet-type mask pack has a support capable of supporting the emulsion so that it can act on the skin for a long period of time as compared with general cosmetic lotion, and by enhancing the skin penetration effect of the skin effect component by the occlusive effect provided by the support This is a form of cosmetic product with excellent moisturizing effect and skin care effect.

The sheet of the cosmetic mask pack generally uses plant cellulose fiber or synthetic fiber non-woven fabric mainly from cotton or pulp as a fresh water body or mask-like support of cosmetic lotion. Nonwoven fabrics have been widely used as an excellent means of delivering various patch formulations, which are inexpensive and can maintain strength even when immersed in water or a solvent, and do not disintegrate in a fiber structure, and carry cosmetics or medicines. However, the nonwoven fabric used in the past is a fiber structure bonded by using a resin-based adhesive of synthetic fibers, which is not excellent in absorbability and moisture retaining ability of the fiber itself, and lacks absorption ability for various harmful substances accumulated in the skin In addition to fibrous nonwoven fabrics, diverse and unique supports can be used as a fresh water body of cosmetic lotion, and various materials researches have been attempted to differentiate the feeling, feeling of wear, and efficacy.

On the other hand, cellulose produced through microbial cultivation is called bio cellulose or microbial cellulose, and is used as a material for an image processing agent for pharmaceuticals and a mask pack for cosmetics. Generally, the production of bio-cellulose is performed by culturing the cells in a culture medium of fruit juice. The production strains of bio-cellulose are classified into Acetobacter genus, Rhizobium genus, And Agrobacterium. However, the strain with the highest production yield is a microorganism most commonly known as Acetobacter xylidium in Acetobacter (Korean Patent No. 0405776). In order to increase the production yield, a microorganism belonging to the genus Acetobacter, which produces bio cellulose, and a lactic acid bacterium which produces bacteriocin to prevent contamination with germs, (Korean Patent Publication No. 2002-0067226).

Bio cellulose is a polymeric substance produced by culturing a large number of bacteria, particularly an acetobacterium strain, and is produced in the form of a translucent pellicle on a culture medium under constant culture conditions (cf. British Patent No. 2131701 number). Biocellulose is a polymer in which glucose is bonded with β-1,4 as in cellulose obtained from plants, but its fiber width is much smaller than that of plant celluloses, and it has a three-dimensional network structure and is excellent in water retention. It has the same soft texture and smooth fit, so it is more industrially useful than plant cellulose. In addition, in order to apply bio cellulose as a cosmetic sheet mask, it is essential to kill the bacteria injected for fermentation and to remove the culture liquid. Is required.

Skin exfoliation can be divided into chemical and physical. The chemical exfoliation method works by decomposing the desmosome existing between the keratinocytes by proper function of moisture and temperature like proteinase. And a method of using an organic acid such as alpha-hydroxy acid or beta-hydroxy acid.

Natural scouring agents, which are physical exfoliants, include vegetable scrubs made from ground powder such as apricot seeds, walnut shells, almond seeds, peach seeds, animal skin made from crab shells and egg shells. Synthetic systems are classified into organic and inorganic systems. Organic systems include nylon, polyvinyl chloride, and polyethylene. Inorganic systems include silica and alumina. Scrub grains should be rounded and free of grain. Less skin irritation and safety.

Bamboo (Bamboo) is a plant of about 280 species worldwide, and about 70 types of bamboo are native or cultivated in Korea. There are 11 kinds of representative varieties such as paddy field, paddy field, bamboo paddy field, bamboo paddy field, paddy field, dough, island paddy field, paddy field paddy field, It is stiff, and it is stout. Bamboo has been used to treat stroke and hypertension. Especially, it has been used for pneumonia and bronchitis for the purpose of fever, diarrhea, and redness. According to recent reports, hypertension, atherosclerosis, It is used for the treatment of cardiovascular diseases and it is introduced as a good plant for anti-cancer and anti-aging. It is considered that this function of bamboo is closely related to the antioxidant effect. Phytochemicals such as organic acids, dietary fiber, tannins, and benzofuran present in bamboo extracts are expected to contribute to the prevention of diseases in the circulatory system through mechanisms such as antioxidant activity, thrombolytic activity and lipid lowering action.

Accordingly, the present inventor has found that, in order to immediately develop skin mask packs such as water replenishment and exfoliation, bio-cellulose (Biocellulose), which has advantages of soft texture and smooth feeling, is used to improve skin health, As a result, it has been found that bamboo extract can be used instead of fruit juice in the culture solution for fermenting Biocellulose, and when the scrub is combined therewith, And completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic mask pack sheet using bio cellulose, which is fermented using a bamboo extract containing a scrub agent, and a method for producing the same.

In order to accomplish the above object, the present invention provides a bio-cellulose mask pack sheet for cosmetics using bio-cellulose prepared by fermenting microbial cultures in a culture solution containing bamboo extract containing a scrub agent.

Also, the present invention provides a bio-cellulose mask pack sheet for cosmetics using bio-cellulose prepared by fermenting a micro-organism culture in a culture solution containing bamboo extract.

The present invention also provides a method for producing a bamboo plant, comprising: 1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; And 4) killing the injected cells and removing the culture medium. The present invention also provides a method for producing a bio-cellulose mask pack sheet for cosmetics.

The present invention also provides a method for producing a bamboo plant, comprising: 1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; 4) killing the injected cells and removing the culture medium; And 5) maintaining the moisture content of the bio-cellulose sheet subjected to the step 4) at a weight of 1 to 50 times the dry weight of the bio-cellulose sheet, and swelling the bio-cellulose by impregnating the bio-cellulose sheet with the cosmetic lotion; The present invention also provides a method for producing a cosmetic sheet mask pack.

The present invention also provides a method for producing a bamboo plant, comprising: 1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; 4) killing the injected cells and removing the culture medium; And 5) preparing a scrubbing agent; 6) maintaining the moisture content of the bio-cellulose sheet after step 4) at a weight of 1 to 50 times the dry weight of the bio-cellulose sheet, and swelling the bio-cellulose by impregnating the bio-cellulose sheet with the cosmetic lotion; 7) adding a scrub agent to the prepared bio-cellulose; The present invention also provides a method for producing a cosmetic sheet mask pack.

The present invention provides a method for producing bio cellulose by fermenting a bamboo extract as a culture solution using microorganisms containing a scrub agent, and a mask pack sheet for applying the same as a fresh water body and a support of a cosmetic mask pack sheet.

In order to apply the bio cellulose as a cosmetic mask pack sheet, the microcell is injected into a culture solution for fermentation, and bio cellulose is obtained through fermentation. , A method of producing a cosmetic sheet mask pack by injecting a cosmetic lotion after the step of dying the bacteria injected for fermentation, the step of removing the culture liquid, and the dehydration step.

The bio-cellulose mask pack sheet of the present invention is produced by the following steps.

1) preparing a bamboo extract;

2) pre-culturing a strain producing bio-cellulose;

3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; And

4) the step of killing the injected cells and removing the culture medium.

The scrub agent of the present invention is prepared by the following steps.

1) preparing scrub granules;

2) preparing a bead having a coating layer formed thereon;

3) a step of mixing the first and second steps

The scrub cosmetic composition comprises one scrub particle selected from the group consisting of beads containing a jojoba ester, alginate beads, and mixtures thereof in the rose dried powder.

The scrub particles are present in an amount of 0.5 to 5% by weight based on the total weight of the cosmetic composition.

The hardness of the scrub cosmetic composition is 28 to 38 N at 25 캜.

The rose dried powder has an average particle diameter of 0.1 to 1.0 mm and an average particle diameter of the bead of 0.15 to 15 mm.

(PEG), polyvinylpyrrolidone (PVP), hydroxypropylcellulose (HPC), methylcellulose (MC), hydroxymethylcellulose (HMC), hydroxypropylmethylcellulose (HPMC) A coating layer composed of at least one component selected from the group consisting of carboxypolymethylene (Carbopol), gelatin, starch, shellac, and styrene-butadiene copolymer (SB-copolymer) is further formed.

The rose dried powder and the beads containing the jojoba ester are mixed in a weight ratio of 1: 1 to 5.

Roses which can be used in the rose dried powder include roses such as damask roses (Bulgarian roses), white roses, English roses, wing roses, and amarola roses. The groups belonging to roses can be used without limitation, Mask rose (invisible rose), white rose is available.

The biocellulose of the present invention uses bamboo extract as a culture solution. The present invention can also be used as a culture solution containing phytochemicals extracted from bamboo. The kind of bamboo that can be used is not particularly limited to Phyllostachys bamboo, Arundinaria bamboo, Sasa bamboo or Pseudosasa bamboo, but preferably is cotton, It is possible to use leaves, roots, stems and the like of bamboo leaves, bamboo shoots, dough, dwarfs, bamboo shoots, , And the bamboo extracts extracted from leaves and stems are more effective.

In addition, in the present invention, a plant extract containing glucose may be further added in order to increase production efficiency while using bamboo extract as a culture medium, and representative examples thereof include cocoa, corn, and bokbunja extract. Acetic acid may be appropriately added to control the pH of the microbial cells. The pH of the microorganism ranges from 3 to 7, which is suitable for culturing.

The bamboo extract used in the present invention can be produced by a method known in the art, and the method is not particularly limited. Specifically, for example, the stem of bamboo can be processed by a method such as chopping, crushing or pulverizing, extraction filtration with a suitable solvent, and then concentrated and dried. As the solvent to be used at this time, ethanol, water, polyols, etc. may be used alone or in combination.

The culture solution of the biocellulose of the present invention is characterized in that the bamboo extract is contained in an amount of 0.01 wt% or more based on the total weight of the culture solution, and the culture solution may be 100 wt% of the bamboo extract. When the content of the bamboo extract is less than 0.01% by weight, it is difficult to obtain a cosmetic mask showing a skin cosmetic enhancing effect.

The culture of the bio cellulose of the present invention may be carried out by mixing various sources of nitrogen with the bamboo extract so that the nitrogen source is not separately mixed or the yield of the bio cellulose is improved. The nitrogen source is well known in the art, and the range is not particularly limited. Specific examples thereof include yeast extract, ammonium sulfate and the like.

Examples of the microorganism producing the bio cellulose of the present invention include Acetobacter genus, Rhizobium genus, and Agrobacterium genus. In addition, various microorganisms can be mixed and cultured for production efficiency. Most preferably, it is effective to follow Korean Patent Laid-Open No. 2002-0067226 for culturing lactic acid bacterium producing bacteriocin to prevent bacterial contamination from stagnant bacteria and cultivating the mixture in a stationary state.

In order to obtain the design of the cosmetic mask pack sheet using the bio cellulose of the present invention, a bio-cellulose can be directly obtained in a completed design form by intaglio-molding a face design on a tray during culturing.

On the other hand, it is also possible to cultivate bio-cellulose in a flat tray (for example, a rectangular shape), and then cut into a desired face-shaped blade to obtain a finished design.

In order to use the biocellulose of the present invention as a cosmetic mask pack sheet, the injected cells are killed and the culture liquid is removed. For this, it is most effective to repeat the hot water sterilization process.

The cosmetic bio-cellulose mask pack sheet of the present invention is subjected to a dehydration or drying process in order to bring the cosmetic lotion into a predetermined water retention state before impregnating the cosmetic lotion. However, when the cosmetic milky lotion is immersed after the complete drying, the water retention of the biocellulose is deteriorated and the swelling of the mask takes too long or the swelling is insufficient. The most effective way to overcome this is to make the water content in the dewatering process using a centrifugal separator be 1 to 50 times the weight of the dry weight of the biocellulose sheet and then inject the cosmetic lotion, It is most effective to maintain moisture in a weight range of 10 to 20 times the dry weight of the sheet. When the moisture content of the biocellulose sheet is less than the same weight as the dry weight, the swelling time of the mask takes too long or the swelling is insufficient, so that the cosmetic lotion can not be sufficiently drained and the feeling of soft feeling is not obtained. In addition, when moisture of more than 50 times the dry weight is carried, the cosmetic mask sheet can not carry any more cosmetic liquid and can not function as a cosmetic mask sheet.

The cosmetic mask pack sheet using the bio-cellulose containing the scrub agent according to the present invention is a novel mask mask sheet material capable of improving skin health and imparting not only wrinkle, whitening, moisturizing, but also additional skin- .

Hereinafter, the present invention will be described in detail. However, the following examples, reference examples and experimental examples are illustrative of the present invention, and the present invention is not limited thereto.

Reference Example 1. Preparation of bamboo crude extract

The Korean bamboo trees were distributed in Sanjia, Silla, Daejon, Sandae, Jeju Sori, Sobaek and Jaejungjuk, washed with clean water, dried naturally in shade for 15 days and used as a sample for extraction. After drying, 100 ℓ of mixed solvent of ethanol and water was mixed with 3 ㎏ of leaves or stems of each bamboo which was made into small pieces, heated for 10 hours, left at room temperature for 16 hours, purified by 3M filter and stored Respectively. 100 g of the extract was weighed and its dry weight was measured. The extract was diluted with purified water so that the concentration of bamboo extract became 0.5%.

Reference Example 2. Acetobacter xylinum culture

Acetobacter xylidium BRC21, a cellulosic production strain, was first pre-cultured separately.

Acetobacter xylidium BRC21 was cultured using HS medium (glucose 20.00 g / l, yeast extract 5.00 g / l, sodium phosphate 2.70 g / l, citric acid 1.15 g / l) supplemented with 2% glucose as a carbon source. 50 ml of HS medium was dispensed into a 250 ml Erlenmeyer flask, sterilized at 120 ° C for 30 minutes, then inoculated with acetolactamide xylonium BRC21, and cultured at 30 ° C for 38 hours in an incubator.

Reference Example 3. Preparation of Rose Dried Powder

After washing the flowers and leaves of Da Mask rose and white rose, they were dried in a drier at 40 ° C for 5 hours so that the moisture content was 8%, and then they were matured at 110 ° C for 2 hours. The roasted rose was dried in a drier at 65 DEG C for 10 hours. The dried roses were pulverized by a pulverizer to prepare rose dried powder having an average particle diameter of 0.5 mm.

Reference Example 4: Production of beads containing a jojoba ester having a coating layer formed

50 g of Jojoba ester beads (FLORATECH, trade name: FLORASOMES) was added to a centrifugal fluidized bed coater and dissolved in 3 g of hydroxymethylcellulose and 2 g of hydroxymethylcellulose in a mixed solvent of water and ethanol, To form a coating layer coated to a thickness of 8 占 퐉.

Reference Example 5. Production of alginate beads

2% of sodium alginate was dissolved in distilled water to prepare a sodium alginate solution. Then, an aqueous solution of sodium alginate prepared in advance in a 1M polyvalent cation solution (CaCl2) was immediately cured by dropping droplets using a syringe and an atomizer, Beads were formed.

Reference Example 6. Manufacturing of scrub

2% by weight of scrub particles, 1% by weight of butylene glycol and 5% by weight of glycerin, 0.5% by weight of xanthan gum, 1% by weight of PEG-4 olivate as an emulsifier, 2% by weight of cetearyl alcohol % Of C14-22 alcohol, 1% by weight of C12-20 alkyl glucoside and 2% by weight of cetearyl glucoside, 3% by weight of jojoba seed oil as softener, 2% by weight of sweet almond oil, 2% 3% by weight of avocado oil, 3% by weight of squalene, 0.5% by weight of shea butter and 3% by weight of oilseed rape seed oil, 1% by weight of hydrolized conjugate ester and 1% by weight of vegetable oil, 0.01% 0.1% by weight of fermented rice extract, 0.2% by weight of grapefruit seed, 0.3% by weight of willow and 0.2% by weight of hind paw were added and stirred for 10 minutes to obtain a cosmetic composition having a viscosity of 30 N at 25 캜.

The scrub particles were prepared by mixing the citron peel powder prepared in Preparation Example 2 and the beads prepared in Preparation Example 1 at a weight ratio of 1: 2.

Example 1 Production of Fermented Bio-Cellulose Using Bamboo Extract

(V / v), 10% (v / v), 20% (v / v) and 30% (v / v) of bamboo extract were added to the extracts of 20 g / l of glucose and 10 ml / And the rest were filled with distilled water and dissolved and mixed. 50 ml of the production medium was dispensed into a 250 ml Erlenmeyer flask, sterilized at 120 ° C for 30 minutes, and then 5 ml of each of the cultured acetaminophen xylidium BRC21 was inoculated. And cultured at 30 DEG C for 120 hours.

Example 2. Production using bamboo extract and a culture medium supplemented with ammonium sulfate as a nitrogen source

(V / v), 10% (v / v), and 20% (v / v) of ginseng extract were added to 20 g / l of glucose and 10 ml / , And 30% (v / v) were added, and 0.5 g / L of ammonium sulfate was added to the nitrogen source. The rest of the mixture was filled with distilled water and dissolved and mixed. Then, 50 mL of the production medium was dispensed into a 250 mL Erlenmeyer flask, For 30 minutes, and 5 ml of each of the cultured acetobacterium xylenium BRC21 was inoculated and cultured at 30 DEG C for 120 hours in an incubator.

Test Example 1. Comparison of microbial cellulose production yield

Cellulose produced in the culture was recovered in order to compare the production yield of the cellulose produced in Examples 1 and 2 above. In order to remove protein components such as microbial cells present in the cellulosic mass, they were immersed in a 1% NaOH aqueous solution, left at room temperature for 24 hours, and then dipped in a 1% acetic acid solution for neutralization. After the neutralization treatment, it was thoroughly washed with water, dried and weighed to obtain the amount of cellulose produced. The results are shown in Table 1 below.

Example Bamboo extract content (% v / v) The amount of cellulose produced (g / l) One 0 1.78 10 3.89 20 4.01 30 4.55 2 0 2.10 10 4.25 20 5.32 30 5.97

As a result, as shown in Table 1, as the content of bamboo extract was increased, the amount of produced cellulose increased, and when the nitrogen source was added together, it was confirmed that the amount of cellulose produced was higher than that when nitrogen source was not used .

Test Example 2. Confirmation of presence of bamboo ingredient in the mask pack sheet

As in Example 1, 30% of bamboo extract was added to 20 g / l of glucose and 10 ml / l of acetic acid, and the remaining was filled with distilled water and dissolved and mixed. Then, 50 ml of the production medium was dispensed into a 250 ml Erlenmeyer flask, And sterilized. 50 ml of this medium was selected for analysis, and 5 ml of each of the cultured acetobacterium xylenium BRC21 was inoculated and cultured in an incubator at 30 ° C for 120 hours. To measure the content of ginseng components in the produced cellulose, it was finely pulverized without any treatment after the production of cellulose. 5 g of each was taken and concentrated, and then 1 ml of solvent was added. This sample was ultrasonically pulverized and centrifuged to separate it from the solution. This was further purified with a filter for injection to prepare a sample. The content of the phenolic compound was evaluated by changing the Folin-Denis method. Namely, 1 mg of each of the samples of the above Examples and Comparative Examples was mixed with 1 ml of Folin reagent, and the mixture was allowed to stand at room temperature for 3 minutes, mixed with 1 ml of 10% Na2CO3 solution and allowed to stand at room temperature for 1 hour and then absorbed at 700 nm . On the other hand, the content of the carbonyl compound was evaluated as follows. First, a standard curve was obtained in advance. 1 mg of the sample of each of the examples and the comparative examples and 1 ml of blank benzene were mixed in a test tube having a stopper. Then, 1 ml of benzene, 2 ml of DNPH solution and 2 ml of TCA solution are stirred for 1 minute in an agitator, and then heated in a water bath at 60 ° C for 30 minutes. The heated test tube is immediately cooled in an ice water bath for 10 minutes, then 25 ml of pure carbonyl-free alcohol is taken into the test tube and 5 ml of KOH is taken from the cooled test tube. After incubation at room temperature for 10 minutes, the absorbance was measured at 430 nm using a UV-spectrophotometer. The measurement results are shown in Table 2 below.

sample Bamboo stem extract Bamboo leaf extract Reference Example 1 (bamboo stem and leaf mixed extract) Phenolic compound 1.928 10.575 18.064 Carbonyl compound 12.728 18.375 29.620

As shown in Table 2, the phenolic compounds and the carbonyl compounds detected in the bamboo extracts were also detected in the mask pack sheet, and even after the fermentation, the phenolic compounds and the carbonyl compounds in the culture liquid component, including the bamboo extract, And can act on the skin.

Reference Example 7 Sheet Mask Pack (Preparation of Comparative Example 1 and Production Example 1)

Emulsions were prepared with the compositions shown in Table 3 below.

division Raw material name Content (% by weight) Oily component Hydrogenated polydecene 8.0 Dimethicone 0.5 Glyceryl stearate SE / peptide-100 stearate 0.8 Polyglyceryl-3 methyl glucoside distearate 1.0 Aqueous component Purified water Balance Disodium edetate 0.02 glycerin 5.0 Butylene glycol 10.0 antiseptic 0.25 Triethanolamine 0.05 Carbomer 0.05 incense Suitable amount Scrub component Rose dried powder 5.0 Jojoba oil 2.0 Sheer butter 1.0 Jan sword 0.5 PEG-4 olivate 1.0 glycerin 0.5

≪ Preparation method of emulsion >

(1) Oily components were heated to 70 to 75 캜 and mixed uniformly.

(2) The aqueous components were uniformly dissolved and mixed by heating at 70 to 75 占 폚.

(3) The above-mentioned (1) was gradually added with stirring while maintaining the temperature at 70 to 75 ° C, and then cooled to obtain an underwater type emulsion.

The bio-cellulose prepared by adding the bamboo extract in an amount of 30% (v / v) in Example 1 was molded and cut into a face shape, and then impregnated with 20 g of the emulsion prepared in the above Table 3 to obtain After the sheet mask pack was manufactured, a scrub agent was applied.

In addition, a commercially available rayon nonwoven fabric was molded and cut into a face shape, and then impregnated with 20 g of the emulsion prepared in the composition of Table 3 to prepare a sheet mask pack of Comparative Example 1.

Test Example 3. Measurement of Skin Elasticity Improvement in Human Body

The skin elasticity improving effect of the above Production Example 1 and Comparative Example 1 was measured. 30 healthy women aged 30 years or older under the conditions of a temperature of 24 to 26 DEG C and a humidity of 75% were selected and divided into two groups, and the cosmetic sheet mask packs of Production Example 1 and Comparative Example 1 were faced each day for 4 weeks And skin elasticity was measured using a skin elasticity meter (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 4. The results in Table 4 are reported as ΔR8 (R8 (left) -R8 (right)) of Cutometer SEM 575, where R8 values indicate the properties of viscoelasticity.

SAMPLE Skin elasticity effect Production Example 1 0.79 Comparative Example 1 0.12

Experimental Results As shown in Table 4, when using the sheet according to the present invention, the cosmetic composition can be more effectively delivered to the skin, thereby providing a much better skin elasticity effect.

Test Example 4. Evaluation of Product Feeling and Satisfaction

50 test subjects were selected to evaluate the adhesion feeling, softness, post-use moisturizing feeling and overall satisfaction during use of the sheet mask packs of Preparation Example 1 and Comparative Example 1, and the cosmetic sheets of Preparation Example 1 and Comparative Example 1 After using the mask pack alternately, questionnaires were conducted. In the questionnaire, each mask pack was used, and the most preferable sheet mask pack type was selected for the feeling of adhesion during use, softness, moisturizing feeling after use, and overall satisfaction as a sheet mask pack. The results are shown in Table 5 .

division Close feeling during use (persons) Softness in use (persons) Sensitivity after use (persons) Effect of exfoliation after use (persons) Overall satisfaction (persons) Comparative Example 1 10 9 11 52 9 Production Example 1 40 45 44 42 43

Experimental Results As shown in Table 5, in the case of Production Example 1 in which biocellulose cultured with a bamboo extract was applied, the satisfaction with the feeling of adhesion was high in the case of using the general rayon nonwoven fabric of Comparative Example 1, (Comparative Example 1) in which the gelled texture of Comparative Example 1 (Preparation Example 1) was composed of fibers was satisfactory even in the item of softness. In addition, in the questionnaire about the moisturizing effect after use, which is the most important property as a cosmetic sheet mask pack, it was found that the satisfaction level of Manufacturing Example 1 in which ginseng fermented bio-cellulose was applied was high, and satisfaction as a whole cosmetic mask pack material It was found that the bamboo fermented bio-cellulose sheet according to the invention was much better.

Claims (10)

A bio-cellulosic mask pack sheet for cosmetics using bio-cellulose prepared by fermenting microbial cultures in a culture solution containing bamboo extract.
A bio-cellulose mask pack sheet using a bio-cellulose prepared by fermenting a micro-organism in a culture solution containing a bamboo extract containing a scrub agent
The cosmetic bio-cellulose mask pack sheet according to claim 1 or 2, wherein the bamboo extract is contained in the culture liquid in an amount of 0.01 to 100% by weight based on the total weight of the culture liquid.
The cosmetic bio-cellulose mask pack sheet according to claim 1 or 2, wherein the culture liquid further contains a nitrogen source such as yeast extract or ammonium sulfate.
The bio-cellulose mask pack sheet according to any one of claims 1 to 3, wherein the moisture content of the bio-cellulose mask sheet is 1 to 50 times the dry weight of the bio-cellulose sheet before impregnating the cosmetic lotion Cosmetic bio-cellulose mask pack sheet.
[3] The cosmetic bio-cellulose mask pack sheet according to claim 2, wherein the scrub agent comprises one kind of scrub particles selected from the group consisting of beads containing jojoba ester, alginate beads, and mixtures thereof in rose petals.
1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; And 4) killing the injected cells and removing the culture medium; Wherein the mask layer is made of a synthetic resin.
1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; 4) killing the injected cells and removing the culture medium; And 5) maintaining the moisture content of the bio-cellulose sheet subjected to the step 4) at a weight of 1 to 50 times the dry weight of the bio-cellulose sheet, and swelling the bio-cellulose by impregnating the bio-cellulose sheet with the cosmetic lotion; Wherein the mask layer is formed on the surface of the mask.
1) preparing a bamboo extract; 2) pre-culturing a strain producing bio-cellulose; 3) introducing a production strain pre-cultured in bamboo extract to produce bio-cellulose; 4) killing the injected cells and removing the culture medium; And 5) preparing a scrubbing agent; 6) maintaining the moisture content of the bio-cellulose sheet after step 4) at a weight of 1 to 50 times the dry weight of the bio-cellulose sheet, and swelling the bio-cellulose by impregnating the bio-cellulose sheet with the cosmetic lotion; 7) adding a scrub agent to the prepared bio-cellulose; Wherein the mask layer is formed on the surface of the mask.
10. The method of claim 9, further comprising the steps of: 1) preparing scrub granules; 2) preparing a bead having a coating layer formed thereon; 3) preparing the bio-cellulosic mask pack sheet for cosmetics by mixing the first and second steps.