KR20150071805A - Method for predicting meat quantity of Hanwoo - Google Patents

Abstract

The present invention relates to an SNP marker used to predict the conductive substance level in beef, a conductive substance level estimating composition including an agent capable of detecting or amplifying the SNP marker, a microarray or a conductive substance level estimating kit for the beef including the same, and a conductive substance level estimating method including a step of determining a polymorphic area in the SNP marker. The SNP marker according to the present invention is a specific SNP marker capable of estimating the conductive substance level of beef and is used to estimate the conductive substance level which is unpredictable before a slaughter operation, thereby increasing the beef breeding farm income.

Description

{Method for predicting meat quantity of Hanwoo}

The present invention relates to a method for predicting the carcass amount of a Korean beef cattle, and more particularly, to a method for predicting a carcass amount of a Korean beef cattle including a SNP marker capable of predicting a carcass level of a Korean beef cattle and a preparation capable of detecting or amplifying the SNP marker A kit for predicting the conductance of a Hanwoo or a microarray for predicting the conductance of a Hanwoo containing the composition, and a method for predicting a conductor amount of the Hanwoo including a step of determining a polymorphic site of the SNP marker.

In general, the ability to improve the breeding and meat quality of Hanwoo has been totally dependent on quantitative genetics - based statistical breeding approaches. This conventional breeding method of Hanwoo requires a capacity test for a large scale group and it is necessary to select individuals with excellent genetic ability through a later test, so that it takes a long time to improve the ability, The accuracy of the selection is not high and the improvement efficiency is greatly reduced. Carcass traits are also difficult to improve phenotypic characteristics in living cattle and to improve carcass traits when using traditional breeding methods, since accurate measurement is possible only after slaughter. Therefore, it is urgently required to develop a new breeding technique and a breeding strategy to maximize the efficiency of carcass trait improvement, which is the most important improved trait of Hanwoo, and to improve the improvement performance.

Normally, beef is classified into meat quality grade and meat quality grade. The meat quality grade is 1 ++, 1+, 1, 2 or 3 grade according to the degree of muscle fat, meat color, local color, texture and maturity (A), (B), (C), and (D) (outside the class) of the carcass weight, backfat thickness, . Since the price of Korean beef is much higher than other imported meat, domestic cow meat, and beef cattle, it is important for consumers to select, breed and produce high quality meat and meat, In order for Hanwoo to be competitive, it is necessary to develop a technology for early selection and selection of high-grade Korean beef with high productivity by increasing the taste and flavor, to be.

Recent developments in molecular genetics and biotechnology have provided new possibilities for the development of more accurate and robust breeding models for animal genetic improvement. The advanced molecular genetic approach provides new information on breeding improvement projects by providing breakthrough information that can overcome the problems of conventional breeding methods in terms of duration of breeding and accuracy of genetic evaluation. have. In particular, studies on the development and utilization of DNA markers related to economic traits can directly identify differences in genetic abilities between animal species at the molecular level of DNA, making it possible to use more accurate genetic information for breeding estimation and genetic evaluation. DNA markers can be applied basically to animal identification, paternal emotion, breed identification, and household analysis, which are the basis of livestock improvement, but their use as DNA markers associated with economically important traits in livestock is of greater value in breeding improvement do. The major advantage of using economic trait-associated DNA markers for breeding in animals is that most DNA markers are homozygous and follow simple Mendelian genetic forms, so the genotype of an individual can be determined directly, And is able to predict and select the genetic potential of an individual early. In the meantime, we have developed various DNA markers according to the remarkable progress of DNA marker analysis technology, and analyzed the relationship between these markers and major economic traits to detect an associated DNA markers affecting quantitative traits, The development of a method for molecular selection and selection of marker-assisted selection (MAS), which directly selects individuals with a specific genotype, is based on the fact that in a conventional selective breeding system dependent on conventional phenotypes alone, The selective breeding became feasible for the epoch-making of animal breeding improvement.

Under these circumstances, the present inventors have made extensive efforts to develop a genetic marker capable of predicting the conductance of Hanwoo as a result, and as a result, it has been found that SNP (Hapmap59709-rs29021868) present on chromosome 14 can be used as a genetic marker And the present invention has been completed.

One object of the present invention is to provide a SNP marker capable of predicting the amount of conduct of a cattle.

It is another object of the present invention to provide a composition for predicting the amount of carbohydrate in a Korean NKU containing a preparation capable of detecting or amplifying the SNP marker.

Yet another object of the present invention is to provide a kits or a microarray for predicting the conductance of a Hanwoo containing the composition.

It is still another object of the present invention to provide a method for predicting the amount of carbohydrate in a Hanwoo including a step of determining a polymorphic site of the SNP marker.

In one aspect of the present invention, there is provided a single nucleotide polymorphism (SNP) marker which is a single nucleotide polymorphism capable of predicting the amount of conduct of a Korean cattle.

The marker may preferably be all or some of the polynucleotides comprising the SNP (Hapmap 59709-rs29021868) present in the chromosome 14 of Korean cattle, more preferably all or some of the polynucleotides of the polynucleotide consisting of SEQ ID NO: 1 And can be a SNP marker capable of predicting the conductor amount of Hanwoo.

Hapmap59709-rs29021868: atagaacaca aaagacatta aagacatttc cctttggtga aaaatcagt r taaaatttaa aagagtggac caacagagaa tacttggttt ctaatgata (SEQ ID NO: 1)

The term "polymorphism " of the present invention refers to the case where two or more alleles exist in one locus. Of the polymorphic sites, only a single base is different from a polymorphic site, It is called single nucleotide polymorphism (SNP). Preferred polymorphic markers have two or more alleles with a frequency of occurrence of 1% or more, more preferably 5% or 10% or more in the selected population.

The term "allele " of the present invention refers to various types of a gene existing on the same locus of a homologous chromosome. Alleles are also used to represent polymorphisms, for example, SNPs have two kinds of bialles.

The "SEQ ID NO. 1" is a polymorphic sequence comprising a polymorphic site. A polymorphic sequence means a sequence comprising a polymorphic site comprising a SNP in a polynucleotide sequence. The polymorphic site may preferably be the 50 < th > base of the polynucleotide sequence of SEQ ID NO: 1.

According to one embodiment of the present invention, in order to discover genetic traits affecting the conductance of Hanwoo, data of 1,011 Hanwoo later test groups with 32,692 DNA variants derived from Hanwoo were analyzed using the statistical analysis model, The genetic mutation (Hapmap59709-rs29021868), which affects the conductance of the Hanwoo corn, was identified as a result of applying the full linear genome association analysis to the Best Linear Unbiased Prediction (BLUP) -animal model (Fig. 1). Next, to determine the genotype corresponding to the excavated genetic mutation of 867 commercial Hanwoo commercial axes and to test the statistical correlation between the carcass quantity data obtained from the Hanwoo commercial axis and the determined genotype, a linear regression model (Hapmap59709-rs29021868) was related to the conductance of Hanwoo as a result of analyzing the correlation between the genotype and the carcass amount of the genetic mutation by performing a single marker regression using a single marker regression (Fig. 2).

Accordingly, in order to confirm how the genetic mutation affects the conductors, three groups corresponding to the three genotypes (A / A, A / G, or G / G) As a result of measuring the average conductance of each group, the Hanwoo having the A / A genotype at the genetic mutation region has significantly higher conductance than the Hanwoo having the A / G genotype and Hanwoo having the G / G genotype , And the Hanwoo with A / G genotype showed a relatively larger amount of conduct than the Hanwoo with G / G genotype. The difference in carcass weight between Hanwoo with A / A genotype and Hanwoo with G / G genotype was about 25kg (Fig. 3). In addition, the analysis of how the genetic mutation affects the conductance of the Hanwoo 538 (KPN538) strain of the certified cows 538 (KPN538) showed that the Hanwoo having the A / And the G / G genotype, and the difference in the carcass amount of the Hanwoo having the A / A genotype and the Hanwoo having the G / G genotype was about 43 kg (FIG. 4).

Therefore, the SNP marker of the present invention detects A / A, A / G or G / G genotypes that can be represented in the polymorphic site located at the 50 th base of the SNP marker (SEQ ID NO: 1) Hanwoo with A / A genotype shows the most carcass quantity, Hanwoo with G / G genotype shows the least carcass amount, and Hanwoo with A / G genotype has middle level of them It can be predicted that it represents the amount of conductor.

In another aspect, the present invention provides a composition for predicting the conductance of Hanwoo, comprising a preparation capable of detecting or amplifying the SNP marker.

The term "agent capable of detecting or amplifying a SNP marker" means a composition capable of predicting the amount of a conductor in a Korean womb by confirming the polymorphic site of the gene by amplification. Preferably, the SNP marker Quot; means a primer capable of specifically amplifying a polynucleotide of the present invention. The primers used for the SNP marker amplification can be amplified using appropriate conditions in suitable buffers (for example, four different nucleoside triphosphates and polymerase such as DNA, RNA polymerase or reverse transcriptase) and template-directed DNA Stranded oligonucleotide which can serve as a starting point of synthesis. The appropriate length of the primer may vary depending on the intended use, but is usually 15 to 30 nucleotides. Short primer molecules generally require a lower temperature to form a stable hybrid with the template. The primer sequence need not be completely complementary to the SNP marker but should be sufficiently complementary to hybridise with the SNP marker and preferably comprise the polynucleotide sequence of SEQ ID NO: 2 or 3, It does not.

The term "primer" of the present invention means a base sequence having a short free 3 'hydroxyl group and can form base pairs with a complementary template, It means a short sequence functioning as a point. The primer can initiate DNA synthesis in the presence of reagents and four different nucleoside triphosphates for polymerization reactions (i.e., DNA polymerase or reverse transcriptase) at appropriate buffer solutions and temperatures. At this time, the PCR conditions, the lengths of the sense and antisense primers can be modified based on those known in the art.

The primers of the present invention can be chemically synthesized using the phosphoamidite solid support method, or other known methods. Such nucleic acid sequences may also be modified using many means known in the art. Non-limiting examples of such modifications include, but are not limited to, methylation, "capping ", replacement of natural nucleotides with one or more homologues, and modifications between nucleotides, such as uncharged linkers, such as methylphosphonate, Phosphoamidates, carbamates, etc.) or charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.).

In another aspect, the present invention provides kits for predicting the conductance of a Hanwoo containing the composition. The kit may be an RT-PCR kit or a kit for DNA analysis (e.g., a DNA chip).

The kit of the present invention can predict the amount of conduct of Hanwoo by confirming the SNP marker, which is a marker for predicting the carcass amount of Korean cattle, by amplification or by checking the expression level of SNP marker with the mRNA expression level. As a specific example, in the present invention, a kit for measuring the mRNA expression level of the SNP marker for predicting the carcass amount of Korean beef cattle may be a kit containing essential elements necessary for performing RT-PCR. In addition to the respective primer pairs specific for the genes of the SNP markers, the RT-PCR kit also includes a test tube or other appropriate container, reaction buffer (pH and magnesium concentrations vary), deoxynucleotides (dNTPs) Enzymes such as Taq polymerase and reverse transcriptase, DNase, RNAse inhibitors, DEPC-water, sterile water, and the like. It may also contain a primer pair specific for the gene used as a quantitative control. Also, preferably, the kit of the present invention may be a kit for predicting the carcass amount of a Hanwoo containing essential elements necessary for carrying out a DNA chip. DNA chip kits are those in which nucleic acid species are attached in a gridded array on a generally flat solid support plate, typically a glass surface not larger than a slide for a microscope, and nucleic acids are uniformly arranged on the chip surface, Hybridization reaction occurs between the nucleic acid on the surface and the complementary nucleic acid contained in the solution treated on the surface of the chip to enable a mass parallel analysis.

In another aspect, the present invention provides a microarray for predicting the conductance of a Hanwoo containing the polynucleotide of the SNP marker for predicting the conductance of the Hanwoo.

The microarray may comprise DNA or RNA polynucleotides. The microarray comprises a conventional microarray except that the polynucleotide of the present invention is contained in the probe polynucleotide.

Methods for producing microarrays by immobilizing probe polynucleotides on a substrate are well known in the art. The probe polynucleotide means a polynucleotide capable of hybridizing, and means an oligonucleotide capable of binding to the complementary strand of the nucleic acid in a sequence-specific manner. The probe of the present invention is an allele-specific probe in which a polymorphic site exists in a nucleic acid fragment derived from two members of the same species and hybridizes to a DNA fragment derived from one member but does not hybridize to a fragment derived from another member . In this case, the hybridization conditions show a significant difference in the intensity of hybridization between alleles, and should be sufficiently stringent to hybridize to only one of the alleles. This can lead to good hybridization differences between different allelic forms. The probe of the present invention can be used for a method of detecting alleles and predicting the amount of carcass in Hanwoo. The determination method includes detection methods based on hybridization of nucleic acids such as Southern blot, and may be provided in a form pre-bonded to a substrate of a DNA chip in a method using a DNA chip. The hybridization can usually be performed under stringent conditions, for example, a salt concentration of 1 M or less and a temperature of 25 ° C or higher. For example, conditions of 5x SSPE (750 mM NaCl, 50 mM Na Phosphate, 5 mM EDTA, pH 7.4) and 25-30 < 0 > C may be suitable for allele-specific probe hybridization.

The process of immobilizing the probe polynucleotide on the substrate associated with the carcass amount prediction of the present invention can also be easily carried out using this conventional technique. In addition, hybridization of nucleic acids on a microarray and detection of hybridization results are well known in the art. The detection can be accomplished, for example, by labeling the nucleic acid sample with a labeling substance capable of generating a detectable signal comprising a fluorescent material, such as Cy3 and Cy5, and then hybridizing on the microarray and generating The hybridization result can be detected.

(A) amplifying the SNP marker of SEQ ID NO: 1 from DNA of a sample derived from a Korean native cattle; And (b) determining the nucleic acid sequence of the 50 th base of the amplified SNP marker.

The sample may be, but is not limited to, hair, urine, blood, various body fluids, separated tissues, separated cells or saliva, and the like.

The step of amplifying the polymorphic site of the SNP marker from the DNA of step (a) may be any method known to those skilled in the art. For example, the target nucleic acid can be obtained by PCR amplification and purification thereof. Other ligase chain reaction (LCR) (Wu and Wallace, Genomics 4, 560 (1989), Landegren et al., Science 241, 1077 (1988)), transcription amplification (Kwoh et al., Proc. Natl. Acad. Sequence amplification based on nucleic acids (NASBA) can be used as well as self-sustaining sequence replication (Guatelli et al., Proc. Natl. Acad. Sci. USA 87, 1874 (1990)).

Determination of the nucleic acid sequence of the SNP marker of step (b) of the above method can be performed by sequence analysis, microarray hybridization, allele specific PCR, dynamic allele-specifichybridization , DASH), PCR extension analysis, PCR-SSCP, PCR-RFLP analysis or TaqMan technique, SNPlex platform (Applied Biosystems), mass spectrometry (e.g. MassenRAY system from Sequenom), mini- Be performed using Bio-Plex system (BioRad), CEQ and SNPstream system (Beckman), Molecular Inversion probe array technology (e.g. Affymetrix GeneChip), and BeadArray Technologies (e.g. Illumina GoldenGate and Infinium assay) But is not limited thereto. One or more alleles in the SNP marker can be identified by the methods described above or other methods available to those skilled in the art to which the invention pertains. The determination of the nucleic acid sequence of such SNP markers can be performed preferably through a DNA chip.

The term "DNA chip" of the present invention means one of DNA microarrays capable of confirming each base of hundreds of thousands of DNAs at a time.

The TaqMan method comprises the steps of (1) designing and preparing a primer and a TaqMan probe to amplify a desired DNA fragment; (2) labeling probes of different alleles with FAM dyes and VIC dyes (Applied Biosystems); (3) performing PCR using the DNA as a template and using the primer and the probe; (4) after completion of the PCR reaction, analyzing and confirming the TaqMan assay plate with a nucleic acid analyzer; And (5) determining the genotype of the polynucleotides of step (1) from the analysis results.

The sequencing analysis can be performed using a conventional method for nucleotide sequencing, and can be performed using an automated gene analyzer. The allele-specific PCR means a PCR method in which a DNA fragment in which the SNP is located is amplified with a primer set including a primer designed with the base at the 3 'end at which the SNP is located. The principle of the above method is that, for example, when a specific base is substituted by A to G, an opposite primer capable of amplifying a primer containing the A as a 3 'terminal base and a DNA fragment of an appropriate size is designed, In the case where the base at the SNP position is A, the amplification reaction is normally performed and a band at a desired position is observed. When the base is substituted with G, the primer can be complementarily bound to the template DNA, And the amplification reaction is not performed properly due to the inability of complementary binding at the terminal. DASH can be performed by a conventional method, preferably by a method such as Prince et al.

On the other hand, the PCR extension analysis first amplifies a DNA fragment containing a base in which a SNP is located to a pair of primers, inactivates all the nucleotides added to the reaction by dephosphorylation, and adds a specific extension primer, a dNTP mixture, Followed by primer extension reaction by adding digoxin nucleotide, reaction buffer, and DNA polymerase. At this time, the extension primer has a base immediately adjacent to the 5 'direction of the base in which the SNP is located at the 3' terminus, and the nucleic acid having the same base as the dodecoxynucleotide is excluded in the dNTP mixture, and the dodecoxynucleotide indicates the SNP Base type. For example, when dGTP, dCTP and TTP mixture and ddATP are added to the reaction in the presence of substitution from A to G, the primer is extended by the DNA polymerase in the base in which the substitution has occurred, The primer extension reaction is terminated by ddATP at the position where the base first appears. If the substitution has not occurred, the extension reaction is terminated at the position, so that it is possible to discriminate the type of the base representing the SNP by comparing the lengths of the extended primers.

At this time, as a detection method, when the extension primer or the dideoxy nucleotide is fluorescence-labeled, the SNP is detected by detecting fluorescence using a gene analyzer (for example, Model 3700 of ABI Co., Ltd.) used for general nucleotide sequence determination And when the unlabeled extension primer and the didyxin nucleotide are used, the SNP can be detected by measuring the molecular weight using MALDI-TOF (matrix assisted laser desorption ionization-time of flight) technique.

Preferably, the Hanwoo having the genotype of the 50 th base, which is the SNP site determined in the step (b), is predicted to have a larger amount of conductance than the Hanwoo having the genotype A / G or G / G, It can be predicted that the amount of cargo is higher than that of Hanwoo which is G / G, although the carcass amount of the A / G Hanwoo which is the genotype is less than that of Hanwoo which is A / A. It can be predicted that the amount of conductor is smaller than that of A / A or A / G Hanwoo.

The SNP markers of the present invention are specific SNP markers for predicting the carcass quantity of Hanwoo, and can be used as a means for predicting the amount of carbohydrate in an unpredictable Hanwoo before slaughter, will be.

FIG. 1 is a graph showing the results of finding genetic traits affecting the carcass quantity of Korean beef cattle in Hanwoo beef cattle test group.
FIG. 2 is a graph showing the results of analyzing the correlation between the genotype and the amount of the conductor of the SNP marker (Hapmap59709-rs29021868) in the Hanwoo commercial axis by a single marker regression analysis using a linear regression model.
3 is a graph showing changes in the amount of conductors according to the genotype (A / A, A / G or G / G) for the SNP marker (Hapmap59709-rs29021868)
4 is a graph showing changes in the amount of conductors according to the genotype (A / A, A / G or G / G) for the SNP marker (Hapmap59709-rs29021868) in the Hanwoo of the certified cows 538 (KPN538)

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1: Hanwoo Black Wu Amount of conductor  relation DNA Marker  excavation

In order to identify genetic traits affecting the carcass quantity of Hanwoo, data of 1,011 Hanwoo test groups with 32,692 DNA variants derived from Hanwoo were analyzed using the following optimal linear uncompensated model (Best Linear Unbiased Prediction (BLUP) -animal model) to perform a genome wide association study (Fig. 1). The p-value of Bonferroni correction (0.000001) was applied to test statistical significance.

BLUP - animal model: y = Xb + Za + e

In the above formula

y represents a phenotypic vector for a fixed effect including sex and age at the time of conduction;

X represents the incidence matirx for the SNP,

b represents the regression coefficient vector for 32,692 SNP genotypes,

Z represents the incidence matrix for the animal effect,

e represents the vector of the residual.

As shown in Fig. 1, a genetic mutation (Hapmap59709-rs29021868), which is a kind of SNP present on chromosome 14 of Korean cattle, was detected as a genetic trait associated with the conductance of Hanwoo.

Example  2: Hanwoo On the commercial axis Amount of conductor  relation DNA Marker  Verification

Whether the genetic variation detected in Example 1 (Hapmap59709-rs29021868) is actually related to the amount of conduct of Hanwoo was verified for Hanwoo commercial axis.

Specifically, the genotype of the detected genetic variation (Hapmap59709-rs29021868) was determined on 867 Korean commercial axis. The genotypes were determined by performing a Real-Time PCR reaction using a TaqMan probe (Applied Biosystems, Korea) and ABI 7500 Real-Time PCR system as follows: 2 μl of DNA (50 ng / (SEQ ID NOS: 2 and 3), 10 μl of TaqMan Master mix (Applied Biosystems, Korea), and 6 μl of distilled water were added to each well to obtain 20 μl of a mixture. Real-time PCR was then performed at 95 ° C for 5 minutes and 40 cycles (60 ° C for 30 seconds and 72 ° C for 1 minute), and the genotype was determined by melting temperature analysis.

Sense primer: 5'-atagaacaca aaagacatta-3 '(SEQ ID NO: 2)

Antisense primer: 5'-tatcattaga aaccaagtat-3 '(SEQ ID NO: 3)

In order to test the statistical correlation between the determined genotypes and the carcass quantity data obtained from the Hanwoo commercial axis, a single marker regression using the following linear regression models was performed to determine the genotype of the genetic mutation And the amount of conductor was analyzed (FIG. 2).

Linear regression model: y = Xb + e

In this formula,

y represents a phenotypic vector for a fixed effect including sex and age at the time of conduction;

X represents the incidence matirx for the SNP;

b represents the regression coefficient vector for the single SNP genotype; And

e represents the vector of the residual.

As shown in FIG. 2, it was confirmed that the genetic variation detected in Example 1 was closely related to the amount of conductors in the Hanwoo commercial shaft.

The results of Examples 1 and 2 indicate that SNPs (Chapmap 59709-rs29021868) existing on chromosome 14 of Hanwoo can be used as markers capable of predicting the amount of carcasses in Hanwoo.

Example  3: Hanwoo On the commercial axis  Genetic variation Amount of conductor  Association analysis

The Hanwoo commercial axis was classified into three groups according to the genotype (A / A, A / G or G / G) of 867 pairs of commercial axis SNP markers (Hapmap59709-rs29021868) determined in Example 2, The average amount of conductors of the commercial axis of Hanwoo included in the group was calculated and the changes in the amount of conductors according to the genotypes of the SNP markers were compared (FIG. 3). At this time, based on the average amount of conductors of the commercial shaft of the 867 Korean beef cattle, the increased and decreased values were expressed.

As shown in FIG. 3, in the Hanwoo commercial axis, the Hanwoo having the A / A genotype showed an average carcass amount of 440.6 kg, the Hanwoo having the A / G genotype had the average carcass amount of 428.9 kg, and the G Hanwoo with / G genotype showed average carcass amount of 414.3kg. When the measured values were compared with the average conductor amount (427.9 kg) of the Hanwoo commercial axis, the average amount of carcasses of A / A genotype was increased to 12.7 kg, and the average of A / G genotype of Hanwoo was 0.96 kg , Whereas the carcass amount of G / G genotype was decreased by 13.6kg.

Therefore, Hanwoo with A / A genotype showed significantly higher carcass quantity than Hanwoo with A / G genotype and Hanwoo with G / G genotype. Hanwoo with A / G genotype showed more carcass than Hanwoo with G / The average carcass weight of Hanwoo with A / A genotype and Hanwoo with G / G genotype were 25kg on average.

Example  4: Assurance sushi  538 ( KPN538 ) Household Hanwoo  Genetic variation Amount of conductor  Association analysis

(A / A, A / G or G / G) for the SNP marker (Hapmap59709-rs29021868) on Hanwoo of the certified cows 538 (KPN538) family in addition to the above Example 3, , The average commercial carcass axis of the Hanwoo commercial shaft included in each group was calculated and the change in the carcass amount according to the genotype of the SNP marker was compared (FIG. 4). At this time, based on the average conductor amount of Hanwoo of the assurance dunn 538 (KPN538) family, the increase and decrease values are indicated.

As shown in Fig. 4, Hanwoo having A / A genotype shows an average carcass amount of 485.7 kg, Hanwoo having A / G genotype shows average carcass amount of 467.2 kg, , And Hanwoo with G / G genotype showed average carcass amount of 442.9kg. In addition, when the measured values were compared with the average conductor amount (465.3 kg) of the Hanwoo of the assured dorsal root gusset 538 (KPN538), the average amount of conductors of the A / A genotype was increased to 20.41 kg, It was found that the average amount of cargoes with G genotype increased by 1.94 kg, while that of G / G genotype decreased by 22.36 kg.

Therefore, in Hanwoo 538 (KPN538), Hanwoo with A / A genotype has significantly higher carbohydrate than Hanwoo with A / G genotype and Hanwoo with G / G genotype. , And that the Hanwoo with A / G genotype showed a relatively larger amount of cargo than the Hanwoo with G / G genotype. In particular, the Hanwoo 538 (KPN538) strain of Hanwoo steers was found to be significantly more effective, and the difference in carcass weight between Hanwoo with A / A genotype and Hanwoo with G / G genotype was 43kg on average.

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Method for predicting meat quantity of Hanwoo <130> PA131074 / KR <160> 3 <170> Kopatentin 2.0 <210> 1 <211> 99 <212> DNA <213> Artificial Sequence <220> <223> Hapmap59709-rs29021868 <400> 1 atagaacaca aaagacatta aagacatttc cctttggtga aaaatcagtr taaaatttaa 60 aagagtggac caacagagaa tacttggttt ctaatgata 99 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 2 atagaacaca aaagacatta 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 3 tatcattaga aaccaagtat 20

Claims (10)

A single nucleotide polymorphism (SNP) marker for predicting the carcass amount of Hanwoo which is represented by SEQ.
A composition for predicting the amount of carbohydrate of Hanwoo comprising the agent according to claim 1 capable of detecting or amplifying the SNP marker for predicting the conductor amount of the Hanwoo.
3. The method of claim 2,
Wherein the agent is a primer having a nucleotide sequence of SEQ ID NOs: 2 and 3.
A kit for predicting carcass amount of Hanwoo containing the composition of claim 2 or 3.
5. The method of claim 4,
Wherein the kit is an RT-PCR kit or a DNA chip kit.
A microarray for predicting the conductance of a Hanwoo including the SNP marker of claim 1.
(a) amplifying the SNP marker of SEQ ID NO: 1 from the DNA of a sample derived from a cattle; And
(b) determining the nucleic acid sequence of the 50 th base of the amplified SNP marker.
8. The method of claim 7,
Wherein the Hanwoo having the genotype of the 50th base of the SNP marker is A / A is judged to have a larger amount of conductors than Hanwoo having the genotype of A / G or G / G.
8. The method of claim 7,
Wherein the genetic form of the 50th base of the SNP marker is A / G, and the amount of the conductor is smaller than that of the genotype A / A, and the amount of the conductor is determined to be larger than that of G / G.
8. The method of claim 7,
Wherein the genetic form of the 50 th base of the SNP marker is G / G, and the amount of the carbohydrate is determined to be smaller than that of the genetically modified Hanwoo which is A / A or A / G.

Images