KR20150061506A - Composition comprising γ-oryzanol derived from danmi - Google Patents

Abstract

The present invention relates to a process for the preparation of gamma-orizanol, comprising the step of extracting gamma-orzanol which is a functional lipid soluble ingredient from a starch, a composition comprising a dicotyledon extract containing said ingredient, and uses thereof.
The present invention relates to a method for preparing gamma-oryzanol from a dicotyledonous extract, wherein the dicotyledonous extract according to the production method of the present invention contains a large amount of gamma-oryzanol in various amounts as compared with other varieties such as common meats, Various applications to compositions having storage stability are possible.

Description

Composition comprising γ-oryzanol derived from daphnia {Composition containing γ-oryzanol derived from Danmi}

The present invention relates to a process for the preparation of gamma-orizanol, comprising the step of extracting gamma-orzanol which is a functional lipid soluble ingredient from a starch, a composition comprising a dicotyledon extract containing said ingredient, and uses thereof.

Gamma-oryzanol (γ-oryzanol) is a chemical that can be extracted not only from rice bran oil but also from barley and corn. It is mainly used as an antioxidant to prevent food from deteriorating. White to pale yellow crystalline powder with little or no unusual odor. It is hardly soluble in water, but it is very soluble in chloroform and ether. It is characterized by being resistant to heat or light rays as compared to other antioxidants.

On the other hand, it is known that such gamma-orizanol is mainly contained in rice bran and the like. However, since there are only about 3 to 5 types of gamma-orizanol solely in common meals and the like, the economical efficiency in using gamma- There were many difficulties such as.

Therefore, the present inventors have made intensive efforts to produce a large amount of gamma-orzanol from natural products, and as a result, they found that gamma-orzanol is contained in a high content in a freshly grown rice cultivar.

It is an object of the present invention to provide a process for producing gamma-orzanol which comprises the step of extracting gamma-orizanol from a starch.

Another object of the present invention is to provide a composition for antioxidation which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition, a food composition, and a cosmetic composition comprising gamma-orzanol-enriched gamma-oryzanol, which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.

In one aspect of the present invention, the present invention provides a method for producing gamma-orzanol, comprising the step of extracting gamma-orizanol from a starch.

In the case of gamma-oryzanol, only a small amount of 3 to 5 species was detected in other general meals and the utilization thereof was not active. However, the inventors of the present invention have for the first time found out that when extracted from a starch, fat-soluble components such as various fatty acids and lipid-soluble carotenoids such as lutein and beta-carotene, especially gamma-oryzanol, are contained in large quantities. The strong antioxidant activity of fat-soluble ingredients can be utilized for various purposes.

As used herein, the term "monolith (Danmi)" is rice (Oryza Sativa L.), and a variety protection application has been registered (breed protection decision notice No. 2010-394). The major morphological characteristics of the plant are: (i) the leaves are green, the leaves are medium in length and width, (ii) the thickness and strength of the stem are thick and strong, and the tillering is common. In addition, (iii) the landing density of the ears is loose, the rock is uncommon, the flaking does not work well, and (iv) the barge and prickles are yellowish white.

The term " gamma-oryzanol "in the present invention refers to a substance present in the oil of rice bran oil, rice germ oil, corn oil and other cereals as a ferrous acid of triterpene alcohol (3-methoxy- - hydroxycinnamic acid) ester. The pharmacological action of γ-orzanol has been described in terms of pharmacological effects such as growth promotion, mitigation of autonomic nerve ataxia, gonorrhea stimulation, promotion of sebum secretion, promotion of blood flow, Absorption, inhibition of tyrosinase activity, and the like, and is also known as a substance with high safety. Therefore, it can be widely used as a drug, a cosmetic, a food additive or an animal growth promoting agent.

In the present invention, gamma-oryzanol, which is a fat-soluble ingredient, is prepared by dissolving a crude rice hull, rice or a pulverized product thereof in an amount of about 2 to 20 times, preferably about 3 to 5 times the dry weight of a fat- . Specific examples of the solvent include benzene, toluene, acetone, carbon tetrachloride, hexane, dichloromethane, chloroform, ether, methylene, ethyl acetate and the like. More preferably, the oil soluble or nonpolar solvent may be hexane.

The extraction temperature is 20 to 100 ° C, preferably at room temperature, and the extraction period is about 12 to 4 days, preferably 1 day, using extraction methods such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction . Preferably, the extract is continuously extracted one to five times by cold extraction, filtered under reduced pressure, and the filtrate is concentrated to a fat-soluble or nonpolar solvent at 20 to 100 ° C, more preferably at room temperature under reduced pressure using a vacuum rotary condenser. The extract may be, but is not limited to, the result obtained. The extract in the present invention may include an extract, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, or any of these adjusted products or purified products.

The gamma-orzanol prepared in the present invention may exist in the form of an extract. Therefore, the gamma-oryzanol component may be present alone, or may be present in a form mixed with other fat soluble components such as lutein, beta-carotene, vitamin E, and the like, but is not limited thereto.

In another aspect, the present invention provides a composition for antioxidation comprising a starch, a pulverized product thereof or an extract thereof as an active ingredient.

Specifically, the starch, the pulverized product thereof or the extract thereof according to the present invention have a strong antioxidative activity and thus can be usefully used for antioxidant use. Preferably, the pulverized product of the present invention, the pulverized product thereof or the extract thereof contains a large amount of gamma-oryzanol component.

The term " anti-oxidative "as used in the present invention refers to a reaction of free radicals or reactive oxygen species (ROS) with high reactivity depending on the oxidative stress caused by intracellular metabolism or ultraviolet The present invention also relates to a method for inhibiting lipid peroxidation in a living body, comprising the steps of: (a) Cancer, vascular circulatory system diseases, and neurodegenerative diseases by seriously damaging the membrane function, decreasing the cell structure and function, and causing mutation of the genetic material. Or extract thereof has an antioxidant effect that suppresses these reactive oxygen species, and thus, it is possible to prevent the aging, cancer, vascular circulation System diseases, neurodegenerative diseases, and the like.

In one embodiment of the present invention, the antioxidative activity of the hexane extract of the present invention was measured by DPPH (1.1-diphenyl-2-picrylhydrazyl) and ABTs (2, 2'-azinodi [3-ethylbenzthiazolne-6-sulfonic acid As a result, the rate of radical production inhibition was measured as 21.8% and 45.3%, respectively, while the inhibition rate of radical formation was 3.9% and 31.8%, respectively. (Example 5).

In another aspect, the present invention provides a pharmaceutical composition for antioxidation enhanced with gamma-oryzanol, which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.

It has been confirmed that the starch, the pulverized product thereof or the extract thereof according to the present invention has a higher content of gamma-oryzanol than the general formula and has a strong antioxidative activity, and thus can be usefully used as a pharmaceutical use of antioxidants. Meanwhile, the gamma-orizanol, antioxidant, starch, pulverized product thereof and extract thereof of the present invention are as described above.

The pharmaceutical composition of the present invention may contain, for administration, a pharmaceutically acceptable carrier, excipient or diluent in addition to the above-mentioned effective ingredient. Specific examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. At this time, the content of the starch, the pulverized product thereof or the extract thereof contained in the composition is not particularly limited, but is preferably 0.001 to 99.9% by weight, and more preferably 0.01 to 50.0% by weight based on the total weight of the composition .

The pharmaceutical compositions of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols or the like, oral preparations, suppositories or sterilized injection solutions according to a conventional method have. More specifically, it can be prepared using a diluent or an excipient such as a filler, a weight agent, a binder, a wetting agent, a disintegrating agent, a surfactant, etc., which are conventionally used for formulation.

The composition of the present invention may be administered in a pharmaceutically effective amount.

The term "pharmaceutically effective amount" as used herein means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will vary depending on the species and severity, age, sex, The type of drug, the activity of the drug, the sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts.

Preferred dosages of the compositions of the present invention will vary depending on the condition and weight of the patient, the severity of the disease, the drug form, the route of administration and the duration. The appropriate total daily dosage may be determined by the treatment within the scope of sound medical judgment, but is generally in the range of 0.001 to 1000 mg / kg, preferably 0.05 to 200 mg / kg, more preferably 0.1 to 100 mg / kg can be administered once or several times a day. The composition is not particularly limited as long as it is an object for antioxidation, and any object can be applied.

The pharmaceutical composition of the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the intended method, and the dose may vary depending on the condition and the weight of the patient, The mode of administration, the route of administration, and the time, but may be appropriately selected by those skilled in the art.

In another aspect, the present invention provides a food composition enriched with gamma-oryzanol, which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.

Since the starch, the pulverized product thereof or the extract thereof according to the present invention has a high content of gamma-oryzanol as a fat-soluble ingredient, the food composition containing the same can be particularly useful as a functional food. Preferably, the food composition of the present invention may have a strong antioxidant activity. Meanwhile, the gamma-orizanol, antioxidant, starch, pulverized product thereof or the extract thereof of the present invention are as described above.

Specifically, when using a gamma-orisol-enriched starch, a pulverized product thereof or an extract thereof of the present invention as a food composition, the starch, the pulverized product thereof or the extract thereof may be directly added or used together with other food or ingredients, And can be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

There is no particular limitation on the kind of the food of the present invention, but examples of the food to which the above-mentioned starch, the ground product thereof or the extract thereof can be added include cereal rice, retort brown rice, meat, sausage, bread, chocolate, candy, , Pizza, ramen noodles, other noodles, gums, dairy products including ice streams, soups, drinks, tea drinks, alcoholic beverages and vitamin complexes, And may include foods used as feedstuffs.

In addition to the above, the food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, , A carbonating agent used in carbonated drinks, and the like. In addition, it may contain flesh for the production of natural fruit juice, artificial fruit juice drinks and vegetable drinks. The food may also be prepared in the form of tablets, granules, powders, capsules, solutions in liquid form, and the like according to known production methods. There are no particular restrictions on other components other than those containing the extract of the present invention as an active ingredient, and may include various conventional flavors or natural carbohydrates as an additional ingredient.

In another aspect, the present invention provides a cosmetic composition enhanced with gamma-oryzanol, which comprises, as an active ingredient, a starch, a pulverized product thereof or an extract thereof.

The pulverized product of the present invention, the pulverized product thereof or the extract thereof according to the present invention has been found to have a significantly higher content of gamma-oryzanol as a lipid-soluble component, and thus can be particularly useful as a cosmetic product. Preferably, in the present invention, it may be added to the cosmetic composition for the purpose of whitening or wrinkle improvement due to the strong antioxidative activity of the ground powder, the ground powder thereof or the extract thereof.

The cosmetic composition of the present invention can be produced in the form of a general emulsified formulation and a solubilized formulation. Examples of the emulsified formulations include nutritive lotions, creams, essences, and the like, and the solubilization formulations include softening longevity. Suitable formulations include, but are not limited to, solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in water, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) A cream, a skin, a lotion, a powder, an ointment, a spray, or a conical stick. It may also be in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, perfumes, surfactants, water, ionic or nonionic emulsifiers, Auxiliaries such as ionic sequestering agents, chelating agents, preservatives, vitamins, blocking agents, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients conventionally used in cosmetic compositions ≪ / RTI >

The present invention relates to a method for preparing gamma-oryzanol from a dicotyledonous extract, wherein the dicotyledonous extract according to the production method of the present invention contains a large amount of gamma-oryzanol in various amounts as compared with other varieties such as common meats, Various applications to compositions having storage stability are possible.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the composition of gamma-orisolol extracted from a rice cake. FIG.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are illustrative of the present invention, and the scope of the present invention is not limited by the following examples.

Example  One: Dummy  40% ethanol Crude extract  Produce

In order to produce a crude extract having the highest content of gamma-orizanol, 1 g of the powdered starch was used as a solvent in 10 ml of 40% ethanol and extracted with stirring for 24 hours. The extracted composition was vacuum-concentrated and powdered.

Example  2: Dummy  70% ethanol Crude extract  Produce

In order to produce a crude extract having the highest content of gamma-orizanol, 1 g of the powdered starch was used as a solvent in 10 ml of 70% ethanol and extracted with stirring for 24 hours. The extracted composition was vacuum-concentrated and powdered.

Example  3: Dummy  80% methanol Crude extract  Produce

In order to produce a crude extract containing gamma-orizanol in a maximum amount, 1 g of the powdered starch was used as a solvent in 10 ml of 80% methanol and extracted with stirring for 24 hours. The extracted composition was vacuum-concentrated and powdered.

Example  4: Dummy Hexane Crude extract  Produce

In order to produce a crude extract containing gamma-orizanol in a maximum amount, 1 g of a powder of a starch was used as a solvent in 10 ml of hexane and extracted with stirring for 24 hours. The extracted composition was vacuum-concentrated and powdered.

Experimental Example  1: Depending on the extraction solvent Dummy  Comparison of extraction rate

The extraction rate was calculated from the yield of the crude starch extract prepared in the above Example. The results are shown in Table 1 below.

Extraction rate Extraction rate according to extraction solvent (%) 40% EtOH
(Example 1) 70% EtOH
(Example 2) 80% MeOH
(Example 3) Hexane
(Example 4) Dummy 10.2 ± 0.2 5.6 ± 0.3 10.2 ± 0.1 7.0 ± 0.0 General beauty
(Japan) 2.5 ± 0.0 2.3 ± 0.2 2.5 ± 0.4 3.1 ± 0.0

As a result, as shown in Table 1, the yield of the crude oil extract was higher than that of the conventional crude oil extract. Especially, 40% ethanol extract of water soluble fraction showed 10.2% of total solids and 2.5% of total solids. In the hexane solvent condition in which the fat soluble solids were extracted, the extraction efficiency was as high as 7.0% Respectively.

Experimental Example  2: Dummy Crude extract Component  analysis

The crude components of the crude hexane crude extract prepared in Example 4 were analyzed and the results are shown in Table 2 below. Here, the general formula is an extract prepared under the same conditions as in Example 4 (hexane crude extract).

Fatty acids mol %) Dummy General Me (Japan) SFA  ( total ) 28.9 21.4 14: 0, Myristic 0.7 0.6 16: 0, Palmitic 23.9 17.7 18: 0, Stearic 2.7 1.8 20: 0, Arachidic 0.7 0.5 22: 0, Behenic 0.3 0.3 24: 0, tetracosanoic 0.6 0.5 MUFA  ( total ) 26.8 40.8 16: 1n-9, 7-Hexadecenoic 0.1 0.1 16: 1n-7, Palmitoleic 0.3 0.2 18: 1 n-9, oleic 25.2 38.9 18: 1n-7, Vaccenic 0.8 1.2 20: 1n-9, Gadoleic 0.4 0.4 22: 1n-9, Erucic 0.0 0.0 PUFA  ( total ) 44.3 38.1 18: 2n-6, Linoleic 42.5 36.9 18: 3n-3, Alpha-Linolenic 1.8 1.2

SFA: Saturated fatty acids, MUFA: Monounsaturated fatty acids, PUFA: Polyunsaturated fatty acids,

As shown in Table 2, (i) the content of saturated fatty acids was 28.9%, which was slightly higher than that of general American (21.4%), but (ii) the saturated fatty acid content was 26.8% (Iii) the content of polyunsaturated fatty acids, known as omega fatty acids, was 44.3% at the end of the diet, which was higher than that of the normal diet (38.1%).

Experimental Example  3: Dummy Crude extract  gamma- Orizanol  Sympathy

Analysis of the crude hexane crude extract prepared in Example 4 above was performed using LC-MS / MS in order to identify the gamma-oryzanol composition of the final product. The analytical instrument was analyzed using a Qual Brower high-sensitivity spectra from Thermo Fisher Scientific. The analytical conditions were #C atoms 30-50, #H atoms 20-80, # 0 atoms 0-10, 5 ppm mass tolerance. Herein, the extracts prepared under the same conditions as in Example 4 (hexane crude extract) were analyzed by the same method as above.

As a result of analysis by LC-MS / MS, as shown in FIG. 1, only three main gamma-oryzanol were found in the general rice, but 10 gamma-orizanol was contained in the rice.

As a result of mass analysis of the ten gamma-oryzanol characteristics contained in the termites, the results were expressed in A to I according to each kind, and the results of expressing the structural formula and MS / MS ion analysis values are shown in Table 4 .

Peak Experimental  mass [M-H] < ^- Formula Identification Theoretycal mass  [M-H] < ^- Accuracy  ( ppm ) Average RT ^* ( min .) MS / MS fragment ions [M-H- Me ] ^- [M-H-2 Me ] ^- [ feruloyl ] ^- feruloyl related ^- A 573.39499 A C ₃₈ H ₅₄ O ₄ 24-methylenecholesterol trans-ferulate 573.39 0.10 31.37 558.39 543.39 193.21 178.08 B 587.41125 B1 - unknown, possible isomer of B2 35.18 572.39 557.67 193.15 177.24-175.04 B2 C ₃₉ H ₅₆ O ₄ Stigmasterol trans-ferulate 587.41 1.14 42.57 572.37 557.51 193.11 178.10 C 601.4273 C1 C ₄₀ H ₅₈ O ₄ Cycloartenol trans - ferulate 601.42 1.78 36.64 586.39 571.46 193.20 177.04 - 75.13 C2 C ₄₀ H ₅₈ O ₄ Cycloartenol cis- ferulate 601.42 1.78 37.79 586.42 571.67 193.13 178.01 - 175.12 D 615.44235 D1 C ₄₁ H ₆₀ O ₄ 24-methylenecycloarthanol trans- ferulate 615.44 0.76 40.39 600.42 575.47 193.29 177.09 - 175.16 D2 C ₄₁ H ₆₀ O ₄ 24-methylenecycloarthanol cis- ferulate 615.44 0.76 44.18 600.44 575.55 193.28 177.19-175.01 E 575.41176 E1 C ₃₈ H ₅₆ O ₄ △ 7-campesterol trans - ferulate 575.41 2.05 39.66 560.41 - 193.33 - E2 C ₃₈ H ₅₆ O ₄ Campesterol trans - ferulate 575.41 2.05 41.20 560.42 545.45 193.12 178.13-175.15 F 589.42709 F1 C ₃₉ H ₅₈ O ₄ △ 7-sitosterol trans - ferulate 589.42 1.46 44.33 574.42 559.53 193.10 178.13 - 177.11 - 175.15 F2 C ₃₉ H ₅₈ O ₄ Sitosterol trans- ferulate 589.42 1.46 45.80 574.40 559.41 193.06 178.02 - 177.13 - 175.11 F3 C ₃₉ H ₅₈ O ₄ Sitosterol cis- ferulate 589.42 1.46 47.34 574.46 559.78 193.13 175.07 - 178.02 G 577.42722 G C ₃₈ H ₅₈ O ₄ Campestanol trans - ferulate 577.42 1.71 46.33 562.42 - 193.06 175.10 H 603.44303 H1 C ₄₀ H ₆₀ O ₄ Cycloartanol trans - ferulate 603.44 1.91 47.13 588.45 - 193.12 174.98 I 591.44359 I C ₃₉ H ₆₀ O ₄ Stigmastanol trans - ferulate 591.44 2.89 51.30 576.46 - 193.13 177.05

Experimental Example  4: gamma- Orizanol  Content measurement

The gamma-oryzanol content of the crude hexane extract prepared in Example 4 was analyzed. The results are shown in Table 4 below. Herein, the general term is the analysis of the gamma-oryzanol content of the extract prepared in the same condition as Example 4 (hexane crude extract).

Dummy General Me (Japan) Carotenoid (占 퐂 / 100 g) Lutein 9 ± 0.8 ^b 5 ± 0.2 ^b trans β-carotene 12 ± 0.3 ^b ND Vitamin E ( mg / 100g) α-Tocopherol 0.94 + 0.07 ^b 0.9 ± 0.1 ^b γ-Tocopherol 0.7 ± 0.05 ^a 0.32 0.02 ^c α-Tocotrienol 0.04 ± 0.002 ^b 0.05 ± 0.01 ^b γ-Tocotrienol 0.11 + - 0.01 ^b 0.13 + 0.01 ^a gamma- Orizanol (? Oryzanol , mg / 100g) 115 ± 8 ^a 46 ± 2 ^c

Mean values ± SD, ^a different letters in the same line are significantly different (p <0.05), ND: not detected

As a result, as shown in Table 4, (i) lutein showed a slightly higher level of 9 μg / 100 g than that of the common meats, and (ii) trans-β-carotene was detected (Ⅲ) Vitamin E content did not show any significant difference between the two groups. On the other hand, (iv) gamma-oryzanol contained 46 mg / 100 g of gamma-oryzanol in the common meals and 115 mg / 100 g of the gamma-oryzanol in the sweet potatoes.

Experimental Example  5: Dummy  Antioxidant activity test of lipid soluble extract

The antioxidant activity of the extract was measured using DPPH and ABTS radical scavenging ability test. Here, the generic name is an extract prepared under the same conditions as in Example 4 (hexane crude extract).

% Inhibition Dummy General Me (Japan) DPPH (1,200 ppm) 21.8 ± 1.0 3.9 ± 2.6 ABTS (1,000 ppm) 45.3 ± 3.6 31.8 ± 0.1

As a result, as shown in Table 5, (i) DPPH antioxidant activity was inhibited by 21.8% of the antioxidant production at a concentration of 1,200 ppm, which was superior to 3.9% Ⅱ) ABTS antioxidant activity at 1,000 ppm was found to be superior to that of the general American at 31.8% inhibition rate by 45.3%.

Example  5: Preparation of capsules

According to the preparation method of known capsules based on the general rules of the Korean Pharmacopoeia, about 500 mg of gamma-oryzanol identified in the above Example was packed into capsules as shown in the following Table 6 to prepare capsules.

ingredient Addition amount (%) Gamma-orizanol 99.95 Vitamin-B1 0.01 Vitamin-C 0.03 Vitamin-B2 0.01 Sum 100

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (11)

A method for producing gamma-oryzanol, comprising the step of extracting gamma-oryzanol from gamma-oryzanol.
The method of claim 1, wherein the gamma-oryzanol is present in the form of an extract.
The process according to claim 1, wherein the gamma-orizanol is extracted with a fat-soluble solvent.
4. The method according to claim 3, wherein the fat-soluble solvent is hexane.
A composition for antioxidation, comprising as an active ingredient an extract from a ground, a pulverized product thereof or an extract thereof.
6. The composition of claim 5, wherein the extract contains gamma-oryzanol.
A pharmaceutical composition for antioxidation enhanced with gamma-orizanol, which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.
A food composition enriched with gamma-oryzanol, which comprises a starch, a pulverized product thereof or an extract thereof as an active ingredient.
The food composition according to claim 8, wherein the composition has antioxidant activity.
A cosmetic composition comprising gamma-oryzanol as an active ingredient, wherein the cosmetic composition comprises a starch, a pulverized product thereof or an extract thereof.
11. The cosmetic composition according to claim 10, wherein the composition has an antioxidative activity.

Images