KR20150052473A - A cosmetic composition comprising subcritical water extracts of camellia sinensis - Google Patents

Abstract

In the present invention, provided is a cosmetic composition including Subcritical water extracts of Camelliasinensis leaf as an active ingredient. Specifically, the Subcritical water extract of Camelliasinensis leaf can be extracted under 1 MPa to 5 MPa between 180 and 240 degree Celsius. Also, the present invention relates to a manufacturing method of Subcritical water extracts, which is allowed to extract Subcritical water extra having good whitening effects and anti-wrinkle activity while having good extract yield from Camelliasinensis leaf wherein the manufacturing method of Subcritical water extracts of Camelliasinensis leaf for preventing or improving wrinkles comprises a step of crushing Camelliasinensis leaf and manufacturing crushed Camelliasinensis leaf.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising a tea leaf extract as an active ingredient,

The present invention relates to a cosmetic composition comprising a natural substance having skin-beauty-functioning property as an active ingredient.

The human skin is the largest organ of the human body which protects the various organs inside the skin from the outside. It has barrier function to prevent the internal moisture and oil components from flowing out of the skin, temperature control function, excretion function , Respiratory function, and so on.

However, not only does the skin's physiological function deteriorate with age, its skin thickness becomes thinner and its function as a skin barrier deteriorates. In addition, external factors such as ultraviolet rays, air pollution, air conditioning and heating, excessive fatigue, stress, The skin is dried by the factor, and such skin dryness also causes the physiological function of the skin to be deteriorated, which causes skin irritation and damage to be easily caused even by an external small stimulus source.

Generally, during the growing period, it is possible to easily cope with the aging factors from inside and outside by various defense systems of the skin. However, as the age increases, the antioxidant system is destroyed by active oxygen species generated by ultraviolet rays, As a result, the defensive ability is significantly reduced. As described above, when oxidative stress is applied to the skin when the defensive ability is lowered, lipid peroxidation, protein oxidation, chain cleavage and abnormal cross-linking of collagen and elastin as elastic fibers, cleavage of hyaluronic acid chain, DNA oxidation Resulting in damage to the biocomponent components. Damage of the above-mentioned bio-constituting components causes skin sagging, roughness, dryness, and the like, which are initial symptoms of wrinkles of the skin, resulting in wrinkles of the skin.

In the past, cosmetics, which were thought to be products used by women, are now used not only as instruments of expressing beauty but also as daily necessities, regardless of whether they are male or female. As the industry develops, , There is a growing interest in functional products that expect special effects and efficacy.

Functional cosmetics currently recognized in Korea are whitening products that help to whiten skin, wrinkle-reducing products that help to improve wrinkles in the skin, and sunscreen products that help protect skin from skin burns and ultraviolet rays .

In general, such functional cosmetics are interpreted as cosmetics that affect not only the cosmetic effect but also the structure and function of the body, and prevent, improve and treat the cosmetics. In particular, recently, the proportion of sales of functional cosmetics has increased markedly from the total sales of cosmetics, and research has been actively conducted to search for new effective ingredients for the human body and to utilize them effectively for skin.

In particular, with the recent increase in the population of elderly people due to the aging of the population and the improvement of quality of life due to economic development, there is an increasing demand for products capable of suppressing or controlling skin wrinkles naturally associated with aging . Particularly, due to an increase in opportunities for social activities of the elderly population, the interest in the beauty of the aged population is increased, while the stimulation of the skin such as ultraviolet rays and the promotion of aging factors are increasing, Demand and usage of functional cosmetics have increased significantly.

Regarding the improvement of the wrinkles of the skin, the reason that the elasticity of the skin is lost and loosened, that is, the wrinkles are formed on the skin appearance, is largely divided into the generation of wrinkles by natural aging and the generation of wrinkles by exposure to ultraviolet rays (photoaging) The mechanism of wrinkling by two causes has not yet been clarified. There are two main types of skin aging closely related to wrinkles. Natural aging, such as wind, temperature, humidity, tobacco smoke and pollution, including chronological aging and exposure to ultraviolet rays over time There is extrinsic aging due to recall factors.

A common phenomenon found in association with the causes of the two wrinkles, namely, natural aging and skin changes due to photoaging is reduction and modification of collagen and elastin, the main constituent proteins in the dermis, and skin caused by reduction of hyaluronic acid, It is elasticity reduction. Particularly, according to the results of studies up to now, it has been known that the reduction and modification of collagen and elastin, which are main proteins in the dermis, are directly involved in the generation of wrinkles and the reduction of elasticity of skin.

The collagen is a major component of the extracellular matrix and is present in extracellular epilepsy as a major substrate protein produced in fibroblasts of the skin. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in the bones and dermis of the skin. In most other structures, it exists as a fibrous inclusion.

The main functions of the collagen are to impart strength and tension to the skin to secure the mechanical rigidity of the skin, to induce the resistance of the connective tissue, to bind tissue, to support cell adhesion, to cell division and differentiation (upon growth or wound healing of the organism) Induction, and the like. In addition, collagen plays an important role in wound healing and is known to promote the synthesis of collagen in the damaged epithelium, thereby allowing the wound to be quickly and without scarring.

Particularly, it has been reported that the reduction of collagen, which accounts for 90% of the dermal layer, is closely related to skin aging. More specifically, the synthesis and degradation of extracellular matrix such as collagen in vivo is appropriately controlled, but the progress of aging or irradiation with ultraviolet light promotes the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen And collagen decomposition by the collagenase degrades skin elasticity and wrinkles are formed.

In addition, various types of skin aging inhibitors known to date are known to have various mechanisms for inhibiting skin aging. Representative examples include collagen production promoters, elastin activators, hyaluronic acid production enhancers, free radical scavengers, cell proliferators, photoaging inhibitors, keratinocyte proliferation inhibitors, humectants, noxious active oxygen production inhibitors, and cell regeneration agents.

Also, as a method for improving skin wrinkles, there has been proposed a method of using a composition for skin external application such as cosmetic or ointment containing collagen. However, since it is difficult to absorb collagen, which is a macromolecule material, percutaneous absorption, it is impossible to expect a moisturizing effect or a wound healing effect, so that it can not exhibit essential skin function improvement and wound healing function It was pointed out.

A method for preventing the formation of wrinkles by an antioxidant such as vitamin C, vitamin E or carotenoid which inhibits the formation or reaction of free radicals, a method for increasing the collagen biosynthesis in the dermis like retinol A method of injecting collagen into skin, or a method of injecting botulinum toxin into skin.

However, vitamin C, vitamin E, carotenoid, and retinol all have a disadvantage that they easily break at room temperature. It is known that retinol has a disadvantage of adverse effect on skin when treatment concentration is high. In addition, in the case of collagen or botulinum toxin, it has been pointed out that the use of the skin is limited or its effect is insufficient due to safety problems such as irritation and redness when applied to the skin, so that the effect of skin function improvement or wound healing can not be expected substantially.

With respect to the raw materials of functional cosmetics, recent researches related to functional materials are mainly focused on natural materials derived from natural materials in conjunction with environment-friendly hot air, and mainly on herb or herbal materials Research is under way, and the need for research is growing.

Tea with coffee, cocoa Camellia ( Camellia senensis ), and it is generally classified into non-fermented tea, which is mainly represented by green tea, oolong tea, which is mainly represented by green tea, Fermented tea represented by tea, and full fermented tea represented by black tea.

Tea has been consumed as a symbol of tea since BC, and in the past it was mainly consumed mainly in oriental countries including Korea, Japan and China, but now it is widely consumed worldwide.

In addition, recently, as the pharmacological mechanism of the various components contained in the tea is gradually found out, its value is recognized by the general public, and the functionality of tea is attracting much attention.

As the awareness about the efficacy and function of tea has spread, the size of tea market and the consumption of tea including green tea have increased, resulting in a significant increase in domestic tea cultivation area. For example, the area of tea cultivation increased from 449 ha in 1985 to 1530 ha in 2000 and to 3800 ha in 2007. As the cultivation area increased, domestic tea production increased from 116 tons in 1985 to 699 tons in 1995, to 1434 tons in 2000 and to 3888 tons in 2007. have.

The consumption of tea is also increasing, but as mentioned above, due to the rapid increase in tea cultivation area and production, problems caused by excessive supply of tea, specifically, the stock of green tea products are increasing. Due to these price declines, domestic green tea business and tea farmers are facing serious difficulties.

In this regard, through the study on the functionality of the tea, it is necessary to study the possibility of expanding the use of the tea by increasing the use of the tea, The urgency is growing even more.

KR 0750870 B KR 0879283 B KR 10-2001-0010368 A

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cosmetic composition containing a natural substance as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition comprising an extract of tea leaf sublingual extract as an active ingredient. Specifically, the extract of tea leaf extract as an active ingredient of the present invention may be a extract of tea leaf extract extract having an extractable index of 3 MPa and 180 ° C to 240 ° C. More specifically, And an asymptotic coefficient of 200 ° C to 220 ° C. More specifically, the tea leaf extract extract may be extracted at an asymptote of 3 MPa and 210 ° C.

The present invention is tea leaf (Camellia The present invention relates to a cosmetic composition for prevention or improvement of wrinkles comprising an extract of tea leaf ash as an active ingredient, characterized in that water is added to pulverized water and extracted at 3 MPa and 180 ° C to 240 ° C.

In addition, the present invention relates to a method for producing an extract of tea leaf ash extract having the highest anti-wrinkle activity and whitening activity from tea leaves as well as the highest extraction yield.

Specifically, the method for preparing the extract of tea leaf extract comprises the steps of pulverizing tea leaves ( Camellia sinensis ) to produce a tea leaf powder; Adding water to the tea leaf leaf pulverized product; And a step of reacting the ground tea leaves with water at 3 MPa and a temperature of 180 ° C to 240 ° C to prepare a tea leaf extract factor extract. Lt; / RTI >

More specifically, the amount of water to be added to the crushed leaf tea leaves is 500 parts by weight to 1,500 parts by weight based on 100 parts by weight of the crushed leaf tea leaves, and the reaction conditions for preparing the tea leaf asphalted leaf extract are 3 MPa and 200 Deg.] C to 220 [deg.] C.

More specifically, the step of preparing the pulverized product of the tea leaves is carried out by drying and pulverizing the tea leaves ( Camellia sinensis ), and the amount of water added to the pulverized tea leaves is 100 parts by weight 900 parts by weight to 1,100 parts by weight as a standard, and the step of preparing the tea leaf extract factor extract may be carried out at 3 MPa and 210 DEG C for 1 minute.

In addition, the present invention provides a tea plant (Camellia manufactured by the manufacturing method sinensis ) leaf extract as an active ingredient. The tea leaf extract factor extract may be extracted with an aside coefficient of 3 MPa and 210 < 0 > C.

In addition, the present invention provides a tea plant (Camellia manufactured by the manufacturing method sinensis ) leaf extract as an active ingredient. The tea leaf extract factor extract may be extracted with an aside coefficient of 3 MPa and 210 < 0 > C.

The inventors of the present invention have confirmed that functional index can be improved compared with the conventional solvent extraction method when a submerged extract is prepared using a water solvent for abundant tea leaves in Boseong area, Jeollanam province, and further research is carried out to improve wrinkles and whitening It has been found that the related effect has the best wrinkle improving activity, specifically the elastase inhibiting activity and the collagen combining ability, and the tyrosinase inhibiting ability, under the specific conditions of 3 MPa and 210 DEG C, It was found that not only the activity decreased but also the extraction yield was the best, and thus the present invention was completed.

In the present invention, a cosmetic composition is a composition used for a human body to clean and fine-tune the human body so as to add charm and change appearance, or to maintain or enhance the health of the skin and hair. Detergents and cosmetics, .

In the present invention, an effective component means a component of a cosmetic composition which is applied to a skin condition to provide prevention, improvement or complementary effect of skin wrinkles.

In the present invention, a supercritical fluid is a material in which the two states of a liquid and a gas are in a critical state in which they can not be distinguished from each other, and when the vapor pressure is under a temperature and a pressure higher than its critical point , And a fluid at a state above the supercritical point. Also, in the present invention, a sub-supercritical fluid means a fluid having almost the same characteristics as a supercritical fluid. The subcritical extraction method refers to an extraction method using a subcritical fluid.

The subcritical fluid may be water, carbon dioxide (CO ₂ ), ethylene, ethane, propane, acetylene or ammonia, preferably economical, free of harmful substances, safely disposable and environmentally friendly, Water which confirms the condition capable of maximizing the extraction efficiency, and more preferably a subcritical water. And the subcritical water means water under subcritical conditions.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising an extract of tea leaf sublingoid extract as an active ingredient

The tea plant is an evergreen tree or evergreen broad-leaved shrub belonging to the genus Camellia . Its scientific name is Camellia sinensis . It is known as a mountainous region, which is bordered by Tibet and China Sichuan Province. It is native to tropical, subtropical and temperate regions, and is now distributed mainly in Korea, Japan, China, India and Southeast Asia. There are many varieties in the above-mentioned tea varieties, and their shapes are greatly different from each other. Thin leaflets cultivated mainly in China and Japan are shrubs that grow to a height of about 2m to 3m in natural state, and the bracts of Indian Assam are about 15m to 30m in height. The leaves of the tea are largely ovate, with a length of 6cm to 20cm and a width of 3cm to 4cm, with dull sawtooth on the edge and pointed end and bottom. The quality of the leaves is hard, slightly thick and glossy on the surface. Depending on the breed, there is a difference in the darkness of the leaf and the difference in the wrinkles. The back of young leaves or young shoots has soft hairs. In Korea, the wild species that grow in Jiri are Chinese domesticated leaves, and in the case of cultivated varieties, domesticated Japanese yabuki species are cultivated.

The tea plant ( Camellia sinensis ) is preferably a tea leaf.

The tea plant ( Camellia sinensis ) leaf ash extract extracts were obtained from tea leaves ( Camellia sinensis ) leaf extract.

In addition, the tea leaf extract factor extract may be used at a subcritical condition of 1 MPa to 5 MPa and 180 ° C to 240 ° C or 2 MPa to 4 MPa and 200 ° C to 220 ° C or 3 MPa and 200 ° C to 210 ° C, Lt; 0 > C to 210 < 0 > C for 1 minute.

In addition, the tea leaf extract factor extract may be a tea leaf extract factor extract, which is characterized by adding water to the ground tea leaves and extracting them under the conditions of 3 MPa and 180 ° C to 240 ° C.

The tea leaf soymilk extract may be extracted with an asymptotic modulus of 3 MPa and 200 ° C to 220 ° C, or with an asymptotic modulus of 3 MPa and 210 ° C.

The conditions of the subcritical extraction method for preparing the subcritical water extract may be such that the pressure of the subcritical water may be 1 MPa to 5 MPa or 2 MPa to 4 MPa or 2.5 MPa to 3.5 MPa or 3 MPa, May be 180 ° C to 240 ° C or 190 ° C to 230 ° C or 200 ° C to 220 ° C or 210 ° C.

The subcritical extraction method for preparing the limulus extract may be performed for 5 seconds to 10 minutes, or 10 seconds to 5 minutes, or 30 seconds to 3 minutes, or 45 seconds to 2 minutes or 1 minute.

The extract of the above-mentioned formula has remarkably excellent thylosinase inhibitory activity, elastase inhibitory activity and collagen synthesis ability as compared with the solvent extract, and in particular, at a pressure condition of 3 MPa and 210 ° C, 150 ° C or 270 ° C Excellent elastase inhibitory activity and collagen synthesis ability were confirmed.

The subcritical extraction may be followed by a filtration step, a concentration step, or a drying step. The filtration step may be performed using a filter paper, and the concentration step may be performed through reduced pressure or the like. And the drying process may be performed by a method such as hot air drying or freeze drying.

As described above, when inhibiting elastase and inducing collagen synthesis, it was confirmed that the effect of preventing or improving wrinkles was excellent, and as a result, it was confirmed through experiments that the cytotoxicity was lower than other extraction conditions, The cosmetic composition of the present invention can be applied to skin requiring a whitening activity or a wrinkle-improving activity, since a substance made of a natural substance such as a tea leaf and water is an effective ingredient and has no side effects and is safe

Accordingly, the cosmetic composition of the present invention may be a whitening cosmetic composition, a cosmetic composition for improving wrinkles, a cosmetic composition for preventing or improving wrinkles, or a cosmetic composition for anti-wrinkles.

The cosmetic composition may contain 0.0001% by weight to 50% by weight, preferably 0.001% by weight to 30% by weight, based on the whole composition, of the extract of the tea leaf extract. In other words, the content of tea leaf extract factor extract should be at least a minimum content to achieve skin-related functionality, specifically, anti-wrinkle effect. However, considering the possibility of application to various formulations, And can be freely adjusted within the above range. At this time, the content of the active ingredient and the content of the additional ingredient may be appropriately adjusted within the above range depending on the content of the ingredients contained in the formulation or cosmetic composition.

The cosmetic composition of the present invention can be used for the external ointment for skin, basic cosmetics, makeup cosmetics, body cosmetics or shaving cosmetics. Examples of the basic cosmetics include essences, lotions, creams, gels, lotions, Cream, emulsion, etc. Examples of the make-up cosmetic may include a foundation, a lipstick, an eye shadow, an eyeliner, a mascara, an eyebrow pencil and a makeup base. Examples of body cosmetics include soaps, liquid cleansing agents, , Sunscreen cream, sun oil, and shaving cosmetics include aftershave lotion and shaving cream.

In the cosmetic composition of the present invention, functional substances having a wrinkle-reducing effect or a whitening effect as well as a functional substance having a UV-blocking or moisturizing effect or a physical property A diluent or the like may be further included.

For example, the cosmetic composition may further contain a perfume, a pigment, a bactericide, an antioxidant, an antiseptic, a moisturizer, a thickening agent, an inorganic salt, or a synthetic polymer material to improve physical properties. An organic pigment, an organic pigment, an ultraviolet absorber, a pH adjuster, an alcohol, a blood circulation accelerator, a cold agent, a limiting agent or purified water may be used as a blending component which may be added.

The cosmetic composition may further include at least one selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The components other than the above-mentioned effective components may be added within the range that does not impair the objects and effects of the present invention.

The cosmetic composition may be in the form of a solution, an emulsion, a viscous mixture or the like, and may be in the form of a liquid, a cream, a lotion, a pack, a foundation, a lotion, a serum, Lt; / RTI >

More particularly, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, , Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

For example, when the formulation of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as the carrier component. In particular, when the formulation of the present invention is a spray, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

When the formulation of the present invention is a solution or an emulsion, a solvent, a solvent, or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

3 MPa and 180 ° C to 240 ° C, or 3 MPa and 200 ° C to 220 ° C, respectively, as compared with the tea leaf extract extract extracted from other subcritical extraction conditions or the tea leaf tea extract produced by other extraction methods, , Or tea leaves extracted with an index of merit of 3 MPa and 210 < 0 > C ( Camellia In the case of the sinensis extract, the extract has excellent thylosinase inhibitory activity, elastase inhibitory activity and collagen synthesis performance improvement effect, and is a natural substance-derived substance, so that side effects are not problematic and safe. ≪ / RTI >

In this respect, the present invention may relate to a method for preparing a tea leaf extract having a whitening activity and a wrinkle improving activity, that is, a wrinkle preventing or improving ability.

The extract prepared according to the method of the present invention has the best anti-wrinkle activity, and the production method of the present invention has the advantage that the extraction yield is the highest as compared with other extraction methods or other extraction conditions .

Specifically, the method for preparing the extract of tea leaf extract comprises the steps of pulverizing tea leaves ( Camellia sinensis ) to produce a tea leaf powder; Adding water to the tea leaf leaf pulverized product; And a step of reacting the ground tea leaves and water at a reaction temperature of 3 MPa and 180 ° C to 240 ° C to prepare a tea leaf extract factor extract. have.

The step of preparing the pulverized tea leaves can be carried out using a conventional terrestrial plant pulverizer, and the tea leaf can be further dried before the pulverized product is manufactured. In this regard, the step of preparing the pulverized tea leaves can be carried out by a method including drying the tea leaves and pulverizing the dried tea leaves.

The natural drying method may be carried out at a temperature of 20 ° C to 30 ° C and a ventilating ventilation method, for example, . ≪ / RTI >

In the step of adding water to the ground tea leaves, the amount of water to be added may be 500 parts by weight to 1,500 parts by weight, or 700 parts by weight to 1300 parts by weight or 900 parts by weight to 1100 parts by weight based on 100 parts by weight of ground tea leaves have.

The water may be ordinary water or distilled water, and the distilled water may be primary distilled water, secondary distilled water or tertiary distilled water.

The step of preparing the extract of tea leaf extract may be carried out by reacting the tea leaf powder and water at a constant temperature and constant pressure for a predetermined time.

More specifically, the pressure condition may be 1 MPa to 5 MPa or 2 MPa to 4 MPa or 2.5 MPa to 3.5 MPa or 3 MPa, and the temperature condition may be, specifically, The temperature conditions for the critical extraction may be 180 ° C to 240 ° C or 190 ° C to 230 ° C or 200 ° C to 220 ° C or 210 ° C. The time for performing the subcritical extraction method for preparing the above-mentioned sublimed extraction extract is 5 seconds to 10 minutes, or 10 seconds to 5 minutes, or 30 seconds to 3 minutes or 45 seconds to 2 minutes, or It can be one minute.

Specifically, the reaction conditions for the step of preparing the tea leafying index extract may be 3 MPa and 200 ° C to 220 ° C or 3 MPa and 210 ° C for 1 minute.

In the subcritical extraction, when a carrier is required, the carrier may be a nitrogen gas. More specifically, the subcritical extraction method may use subcritical water as a subcritical fluid and use nitrogen gas as a carrier.

The above-mentioned sub-critical hydrothermal extraction method solves the problem that excessive cost and process are required to remove the organic solvent of the conventional extraction method and that it can cause environmental problems. In addition, The extraction efficiency is improved by about 1,300% to 200% in comparison with the solvent extraction method which is the conventional extraction method, specifically, when the extraction is carried out under the optimum conditions of 3 MPa and 210 ° C, Yield was obtained.

As described above, the method of producing the tea leaf extract having anti-wrinkle activity by using the limulus coefficient of the present invention has safety and eco-friendliness as compared with the conventional method, and can be performed by a relatively simple process, The anti-wrinkle activity is improved and the yield is also remarkably increased.

The tea tree leaf extract extract of the present invention can improve the esterase inhibitory activity and the collagen aggregation performance, that is, the anti-wrinkle activity and the tyrosinase inhibitory activity, that is, the whitening activity, according to the conditions of the subcritical extraction, The extraction yield can be improved and the whitening activity, the anti-wrinkle activity and the yield can be remarkably improved as compared with the extraction method using conventional organic solvents, and the most popular drinkable tea material and the most safe solvent As a result, it has been confirmed that there is no toxicity due to the result of the experiment, and furthermore, it can be manufactured by a simple method using the abundantly produced tea leaves in the southern part of Korea, which is economically advantageous , It can contribute to the revitalization of the local economy and the improvement of income of the local farmers' The cosmetic composition will be that a very great effect from the industrial aspect.

FIG. 1 is a graph showing the inhibitory effect of tyrosinase according to the kind of each extract according to an embodiment of the present invention, wherein the abscissa indicates the temperature or the pressure in the subcritical extraction condition, the letter indicates the type of the solvent , And the vertical axis indicates the inhibitory activity of tyrosine kinase by an IC50 value.
FIG. 2 is a graph showing the inhibitory effect of elastase according to the kind of each extract according to an embodiment of the present invention, wherein the abscissa indicates the temperature or the pressure in the subcritical extraction conditions, the letter indicates the type of the solvent, And the vertical axis means that the inhibitory effect of elastase is represented by IC50 value.

Hereinafter, examples of the present invention will be described. The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. However, the following Preparation Examples and Examples are merely illustrative examples for the purpose of facilitating understanding of the present invention, and can be modified into various other forms, and the scope of the present invention is not limited to the following Examples.

< Example  1> Manufacture of tea leaf extract

The tea leaf samples were prepared by pulverizing tea leaves collected from Boseong area, Jeollanam - do, naturally dried for 3 days at 20 ℃ ~ 30 ℃ and ventilating conditions.

The subcritical extract of the tea leaves was prepared by adding 20 g of the above ground tea leaves and 200 mL of distilled water to a subcritical extraction device, and then the temperature was adjusted to 90 ° C, 150 ° C, 180 ° C and 210 ° C respectively under the pressure conditions of 1 MPa, 3 MPa and 3 MPa Deg.] C and 240 [deg.] C, respectively, and reacted at the respective pressure and temperature conditions for 1 minute to extract. The extract thus obtained was filtered under reduced pressure using a paper filter (Whatman, No. 2), and the filtrate was concentrated using a rotary vacuum evaporator. The concentrate was dried using a freeze dryer to obtain powder To prepare an extract. The final extracts were stored at -20 ℃ and used in the experiment.

The tea leaf extract was prepared by solvent extraction using water, methanol and ethanol. Specifically, the tea leaf hot-water extract was prepared by immersing 20 g of the ground tea leaves in 200 mL of distilled water and then extracting at 95 ° C for 1 hour. The extract thus obtained was filtered under reduced pressure using a paper filter (Whatman, No. 2), and the filtrate was concentrated using a rotary vacuum evaporator. The concentrate was dried using a freeze dryer to obtain powder To prepare an extract. The final extracts were stored at -20 ℃ and used in the experiment.

The solvent extract of tea leaf tea using the organic solvent was prepared by dipping 20 g of the ground tea leaf in 200 mL of methanol or ethanol for 24 hours at room temperature. The extract thus obtained was filtered under reduced pressure using a paper filter (Whatman, No. 2), and the filtrate was concentrated using a rotary vacuum evaporator. The concentrate was dried using a freeze dryer to obtain powder To prepare an extract. The final extracts were stored at -20 ℃ and used in the experiment.

The extraction yields of the extracts prepared by the above methods were compared with those of the final extracts obtained from the first used tea leaf samples. The results are shown in Table 1 below.

extract
Kinds Subcritical extraction (3M Pa) Solvent extraction 90 ° C 150 ℃ 180 DEG C 210 ℃ 240 ℃ Heat number Methanol ethanol Extraction yield (%) 23.09 23.69 25.7 29.25 25.9 21.44 17.36 16.99

As shown in Table 1, it was confirmed that the subcritical extraction method had a relatively higher extraction yield than the solvent extraction method. In the subcritical extraction method, the extraction yield increased with increasing temperature up to 210 ℃, but the extraction yield decreased at 240 ℃ and 210 ℃ was most suitable. In particular, it was confirmed that the subcritical extraction at 210 ° C improved the yield by about 40% and the yield was improved by about 80% as compared with the solvent extraction method using ethanol, compared with the hot water extraction method having the best extraction yield in the solvent extraction method.

Therefore, it was confirmed that it is most preferable to perform the subcritical extraction under the conditions of the above 3 MPa and 210 캜 in terms of the extraction yield to prepare an extract.

< Example  2> Determination of functional properties of tea leaf extract according to extraction method and extraction conditions

The tea leaf extract prepared in Example 1 was confirmed to have anti-wrinkle activity according to extraction method, extraction solvent and extraction conditions, and further cytotoxicity was confirmed.

2-1. Tyrosinase  Identification of whitening activity through inhibitory activity

In order to confirm the whitening activity of the tea leaf extract prepared in Example 1, tyrosinase inhibitory activity was confirmed as follows.

First, mushroom-derived tyrosinase (Sigma, USA), which is known to be involved in melanin biosynthesis, was used to measure the whitening effect of green tea extract and solvent extract, and the substrate was L-DOPA (3 , 4-Dihydroxyphenylalanine, Sigma) were used. After adding 1 mL phosphate saline buffer (0.1 M, pH 6.8), 0.1 mL sample, 0.3 mL distilled water and 0.1 mL tryrosinase solution (0.1 M PBS, pH 6.8, 500 unit / mL) (Pre-incubation). Tyrosinase inhibitory activity was calculated by adding 0.5 mL of 10 mM substrate solution to the cuvette and measuring the amount of product at 475 nm. The results are shown in Table 2 and FIG.

sample Tyrosinase inhibition activity
(SC50: mg / mL) Name of sample Extraction condition Subcritical extraction
(3M Pa) 90 ° C 2.24 150 ℃ 1.96 180 DEG C 1.88 210 ℃ 1.72 240 ℃ 1.77 Solvent extraction Heat number 2.34 Methanol 2.27 ethanol 2.45

As shown in Table 2, it was confirmed that the inhibitory activity against tyrosinase was superior to the solvent extraction method by the subcritical extraction method. With regard to the subcritical extraction method, the extraction yield was increased with increasing temperature up to 210 ° C., but the extraction yield was decreased at 240 ° C. and 210 ° C. was the most suitable. In addition, with respect to the subcritical extract, the results of tyrosinase inhibition activity according to the pressure conditions in the extraction conditions are shown in Fig. As shown in FIG. 1, when the pressure was increased from 1 MPa to 3 MPa under all the temperature conditions, the inhibition activity was increased, but when the pressure was increased from 3 MPa to 5 MPa, the inhibition activity was rather decreased. 3MPa was found to be suitable.

Therefore, it has been confirmed that it is most preferable to perform the subcritical extraction at the above-mentioned 3 MPa and 210 캜 in order to produce the extract, in relation to the anti-wrinkle activity through the elastase inhibitory activity.

2-2. Elastase  Through inhibition Anti-wrinkle activity  Measure

In order to measure the anti-wrinkle activity of the tea leaf extract factor extract through inhibitory activity against elastase, which is known to be one of the causes of skin wrinkles, the tea leaf extract prepared in Example 1 was assayed for elastase inhibitory activity Respectively.

More specifically, experiments were performed using pig-derived elastase and N-succinyl-1- (Ala) 3-p-nitroanilide as a substrate. First, 60 μL of 50 mM Tri-HCl buffer (pH 8.0) was added to each well of a 96-well plate, and then 10 μL of 10 mM N-succinyl-1- (Ala) 3-ρ-nitroanilide and each of tea leaf extract and After addition of 10 μL of distilled water, the plate was preincubated at 37 ° C for 5 minutes. Then, 10 μL of the elastase prepared at a concentration of 1 unit / mL was added to each well, Lt; / RTI &gt; After the reaction, the inhibitory activity was measured by measuring the absorbance at 415 nm. The IC ₅₀ determined as the result of the measurement was shown in Table 3 and FIG. As a result of confirming the above urusholic acid by the same method, it was confirmed that the IC ₅₀ value was 12.9 / / mL.

sample Elastase inhibition activity
(SC50: 占 퐂 / mL) Name of sample Extraction condition Subcritical extraction
(3M Pa) 90 ° C 454.02 150 ℃ 394.8 180 DEG C 388.5 210 ℃ 371.98 240 ℃ 381.67 Solvent extraction Heat number 461.84 Methanol 402.76 ethanol 424.18

As shown in Table 3, it was confirmed that the inhibitory activity against elastase was superior to the solvent extraction method by the subcritical extraction method. With respect to the subcritical extraction method, the extraction yield was increased with increasing temperature up to 210 ° C., but the extraction yield was decreased at 270 ° C., and 210 ° C. was the most suitable. In addition, regarding the subcritical extract, the results of inhibition of elastase according to the pressure conditions in the extraction conditions are shown in Fig. As shown in FIG. 2, when the pressure was increased from 1 MPa to 3 MPa under all the temperature conditions, the inhibitory activity was increased, but when the pressure was increased from 3 MPa to 5 MPa, the inhibitory activity was rather decreased. 3MPa was found to be suitable.

Therefore, it has been confirmed that it is most preferable to perform the subcritical extraction at the above-mentioned 3 MPa and 210 캜 in order to produce the extract, in relation to the anti-wrinkle activity through the elastase inhibitory activity.

2-3. Collagen Productive  through Anti-wrinkle activity  Measure

In order to confirm the anti-wrinkle activity of the tea leaf extract prepared in Example 1, the collagen production ability was confirmed by measuring the content of collagen released from the cells in a normal human dermal fibroblast (skin cell) .

Specifically, the Sirius red dye binding assay was performed using the CCD-1064 Sk (normal human dermal fibroblast, ATCC CRL-2079, USA). First, the CCD-1064Sk was dispensed into a 24-well plate at a concentration of 1 × 10 ⁵ cells / mL at 300 μL / well, and cultured at 37 ° C and 5% CO ₂ for 24 hours. 1 was added to IMDM (iscove's modified dulbecco's medium) containing 10% fetal bovine serum (FBS), treated with 300 μL each of the extracts at 37 ° C and 5% CO ₂ for 72 hours.

To 600 μL of the medium, 6 mL of 50 μM sirius red solution (0.5 M acetic acid) was added, followed by incubation at room temperature for 20 minutes. After the reaction, centrifugation was carried out at 5000 x g for 20 minutes, and then 200 μL of 0.5 M NaOH was added to the precipitate, and the collagen was eluted for 5 minutes. The eluted collagen dye was transferred to a 96-well plate, and the absorbance at 540 nm was measured to determine the total collagen content released from the cell. The results are shown in Table 4 below.

sample Collagen synthesis on Fibroblast (%) 25 μg / mL 100 μg / mL 500 μg / mL 1000 μg / mL Subcritical
extraction 1 Mpa 90 ° C 103.1 ± 0.6 109.4 ± 1.1 116.5 ± 0.4 123.7 ± 0.7 150 ℃ 106.4 ± 0.7 113.2 ± 1.3 118.7 ± 0.8 125.6 ± 0.7 210 ℃ 107.3 ± 0.5 116.9 ± 1.1 121.7 ± 0.3 128.4 ± 0.7 3 Mpa 90 ° C 105.8 ± 0.8 112.7 ± 0.9 118.4 ± 0.7 125.6 ± 0.6 150 ℃ 107.4 ± 1.2 115.3 ± 0.7 121.1 ± 0.9 127.8 ± 0.2 180 DEG C 108.6 ± 1.2 117.5 ± 0.8 122.8 ± 0.2 129.6 ± 1.2 210 ℃ 106.8 ± 0.7 117.9 ± 0.5 124.6 ± 0.3 133.2 ± 0.7 240 ℃ 107.2 ± 1.3 115.8 ± 0.6 121.4 ± 1.2 128.4 ± 1.4 5 Mpa 90 ° C 104.6 ± 1.5 109.6 ± 0.7 115.7 ± 0.2 119.6 ± 0.6 150 ℃ 106.4 ± 0.7 114.3 ± 1.1 117.9 ± 1.4 123.5 ± 0.3 210 ℃ 107.3 ± 0.9 116.8 ± 0.2 119.2 ± 0.7 125.6 ± 1.3 Solvent extraction Heat number 103.2 ± 0.3 105.8 ± 0.8 114.6 ± 0.6 122.7 ± 0.7 Methanol 105.1 ± 0.9 1118. ± 0.7 118.2 ± 0.3 124.1 ± 0.4 ethanol 104.3 ± 1.1 107.7 ± 0.7 114.5 ± 0.5 119.7 ± 0.9

As shown in Table 4, it was confirmed that the collagen production ability was superior to the solvent extraction method by the subcritical extraction method. With respect to the subcritical extraction method, the extraction yield was increased with increasing temperature up to 210 ° C., but the extraction yield was decreased at 270 ° C., and 210 ° C. was the most suitable. With respect to the subcritical extract, when the pressure was increased from 1 MPa to 3 MPa, the collagen production ability was increased. However, when the pressure was increased from 3 MPa to 5 MPa, the collagen production activity decreased, and 3 MPa Respectively.

Therefore, it has been confirmed that, in relation to the anti-wrinkle activity through the collagen production ability, it is most preferable to perform the subcritical extraction at the above-mentioned 3 MPa and 210 캜 to prepare an extract.

Claims (7)

Camellia crushing a leaf of a sinensis leaf to produce a crushed tea leaf; Adding water to the tea leaf leaf pulverized product; And the step of reacting the ground tea leaves and the water at 3 MPa and 180 ° C to 240 ° C to prepare a tea leaf extract extract
Wherein the extract has a wrinkle preventing or improving ability. The method according to claim 1,
The amount of water to be added to the crushed carnauba leaf is 500 parts by weight to 1,500 parts by weight based on 100 parts by weight of the crushed carob tree powder. The reaction conditions of the step of preparing the carnauba leaf extract factor are 3 MPa and 200 to 220 ° C Which is a condition for preventing or improving wrinkles. 3. The method of claim 2,
The tea plant ( Camellia sinensis) preparing a leaf pulverized product is a tea ipreul dried, is to perform a method of grinding, the tea leaves 900 parts by weight based on the amount of water tea leaf pulverized product 100 parts by weight of crushed added to water to 1100 parts by weight Wherein the step of preparing the extract of tea leaf ash extract is carried out at 3 MPa and 210 캜 for 1 minute. A method for producing a tea tree ( Camellia &lt; RTI ID = 0.0 &gt; sinensis) leaves subcritical water extract wrinkles cosmetic composition for prevention or improvement containing as an active ingredient. 5. The method of claim 4,
Wherein the tea leaf extract is extracted with an ash factor of 3 MPa and 210 캜. A method for producing a tea tree ( Camellia &lt; RTI ID = 0.0 &gt; sinensis) cosmetic composition for whitening comprising the subcritical water leaf extract as an active ingredient. The method according to claim 6,
Wherein the tea leaf extract is extracted with an asymptote of 3 MPa and 210 DEG C.

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