KR20150002016A - Moisturizing composition comprising Panax ginseng polysaccharide and Green tea polysaccharide - Google Patents

Abstract

In the present invention, provided is a moisturizing composition. More specifically, in the present invention provided is a composition of external application to the skin, containing polysaccharide of ginseng and of green tea as active ingredients, so the composition has outstanding effects of recovering damaged skin barrier function and increasing skin moisturizing. The composition of external application to the skin in the present invention comprises 0.001-10 wt% of polysaccharide of ginseng and of green tea for the total weight percentage of each composition and has excellent function which keeps moisturizing skin.

Description

[0001] The present invention relates to a ginseng polysaccharide and a green tea polysaccharide,

The present invention relates to a composition for moisturizing, and more particularly, to a composition containing a ginseng polysaccharide and a green tea polysaccharide as an active ingredient, and having excellent effect of restoring a damaged skin barrier function and increasing skin moisturizing power.

BACKGROUND ART [0002] In the human body, skin functions as a protective film for protecting a living body from the external environment, and serves to prevent the loss of biological components in the human body, that is, water and electrolytes, and to prevent harmful substances from entering the human body from the outside . The skin is divided into the epidermal layer, the dermal layer and the subcutaneous fat layer, and the epidermal layer is composed of keratinocytes and melanocytes. In particular, the stratum corneum (horney layer), which exists at the outermost part of the skin, is in direct contact with the external environment and therefore requires strong resistance against physical, chemical or permeation of substances, At the same time, it should maintain its flexibility by retaining adequate water on its own. This protective function can be maintained only when the stratum corneum composed of keratinocytes is normally formed.

The keratinocytes are characteristic cells in which basal cells proliferating continuously in the lowest epidermis undergo a gradual morphological and functional change and rise to the skin surface. After a certain period of time, old keratinocytes are removed from the skin and new keratinocytes replace their functions. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization". In addition, during keratinization, keratinocytes form natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids) and form stratum corneum, which gives firmness and flexibility to the stratum corneum, barrier function.

However, recently, the risk factors for skin are increasing, and the rate of generation and elimination of the stratum corneum is slowed by the change of dietary life, and the amount of natural moisturizing factor and lipid is decreased by the function of keratinocyte, The number of cases that can not be demonstrated is increasing. Such breakdown of the skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis and psoriasis.

Therefore, in order to maintain proper skin moisture, various studies have been made to supply water from the outside or to prevent water loss from the body, and various moisturizers having moisture retention ability have been developed. As a skin moisturizing agent, it is common to use a substance capable of increasing water retention in the stratum corneum such as ceramide and essential fatty acid and lipid complex (Rawlings AV et al . , J. Invest . Dermatol. , 5, pp731- 741, 1994).

Accordingly, the inventors of the present invention found that, when the ginseng polysaccharide derived from ginseng and the green tea polysaccharide derived from green tea are used at the same time, the synergistic effect effectively restores the damaged skin barrier function and effectively increases the skin moisturizing effect And finally completed the present invention.

Accordingly, it is an object of the present invention to provide a composition which provides excellent skin moisturizing effect by using a ginseng polysaccharide and a green tea polysaccharide simultaneously.

In order to achieve the above object, the present invention provides a composition for external application for skin or a pharmaceutical composition comprising a ginseng polysaccharide and a green tea polysaccharide as an active ingredient.

The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide, thereby exhibiting an effect of restoring damaged skin barrier function and increasing skin moisturizing power. Accordingly, the composition containing the ginseng polysaccharide and the green tea polysaccharide is useful for skin moisturizing, skin barrier function enhancement, and composition for inducing differentiation of dermal keratinocyte, and is useful for preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis Lt; / RTI >

The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide as effective ingredients, thereby providing excellent skin moisturizing effect.

Hereinafter, the present invention will be described more specifically.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. In addition, arabinose, rhamnose, glucose and And galactose constitute side chains. The ginseng used in the present invention is a plant belonging to the genus Ogushi and ginseng. In the present invention, ginseng can be used without limitation in its kind and root length, and both dried and not dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

The green tea polysaccharide used in the present invention is derived from green tea and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used regardless of the type and the number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

The ginseng polysaccharide and the green tea polysaccharide used in the present invention can be produced by a method known in the art, and the method is not particularly limited. Specifically, a green tea polysaccharide or a ginseng polysaccharide can be obtained by preparing a ginseng root or green tea leaf extract with water at 38 to 42 캜, concentrating it, removing the impurities with a filter, adding ethanol dropwise thereto and drying with hot air .

The composition of the present invention may contain the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.005 to 9.5% 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

In the present invention, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. In order to maximize the synergistic effect of the combination of the two components, preferably, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1 .

The composition for moisturizing according to the present invention can be used for enhancing skin barrier function and inducing differentiation of dermal keratinocyte. Therefore, the composition for preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incompleteness of epidermal differentiation Can be usefully used as an external composition for skin.

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Comparative Example 1] Production of ginseng polysaccharide

1 kg of dried white ginseng was pulverized with a blender, and then 10 L of purified water was added thereto, followed by stirring at 38 to 42 ° C in hot water for 24 hours, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 65 g of ginseng polysaccharide.

[Comparative Example 2] Production of green tea polysaccharide

1 kg of first processed green tea leaves were pulverized with a blender, 10 l of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise and the mixture was hot-air dried to obtain 72 g of a green tea polysaccharide.

[Example 1] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 1: 3.

[Example 2] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 1: 1.

[Example 3] Preparation of a mixture of ginseng polysaccharide and green tea polysaccharide

The ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 2 were mixed at a weight ratio of 3: 1.

[Test Example 1] Measurement of promoting effect of keratinocyte differentiation

In order to confirm the skin barrier function and skin moisturizing ability of Examples 1 to 3 and Comparative Examples 1 and 2, the amount of corned envelope (CE) produced upon differentiation of keratinocytes was measured by absorbance.

First, the primary cultured human keratinocytes (HaCaT) (available from Dr. NE Fusenig, Deutsches Krebsforschungszentrum, Heidelberg, Germany) were placed in a culture flask and attached to the bottom. , And the cells were cultured for 5 days until they reached 70 to 80% of the bottom area. The cells were harvested and washed with PBS (phosphate buffered saline). The cells were washed with 10 mM Tris-HCl buffer (Tris-HCl buffer, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) , pH 7.4) was added and sonication was performed for 3 minutes, followed by boiling at 85 ° C for 10 minutes. This was centrifuged at 1200 rpm for 30 minutes, and the separated precipitate was suspended in 1 ml of PBS and the absorbance at 340 nm was measured. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were negative control and positive control, respectively. Test results obtained by adding Comparative Examples 1 to 2 and Examples 1 to 3 to the low calcium concentration (0.03 mM) for each concentration are shown in Table 1 below.

Test substance density The ability to differentiate in keratinocytes (%) Negative control (low calcium solution) 0.03 mM 100 Positive control (high calcium solution) 1.2 mM 215 Comparative Example 1 0.05 ppm 106 0.1 ppm 109 Comparative Example 2 0.05 ppm 101 0.1 ppm 102 Example 1 0.05 ppm 112 0.1 ppm 122 Example 2 0.05 ppm 115 0.1 ppm 123 Example 3 0.05 ppm 115 0.1 ppm 124

As shown in Table 1, it was confirmed that the mixed use of the ginseng polysaccharide and the green tea polysaccharide of Examples 1 to 3 was excellent in the promotion of differentiation of keratinocytes. Therefore, it has been confirmed that the composition of the present invention has enhanced skin barrier function and skin moisture protecting ability.

[Test Example 2] Measurement of filaggrin expression by RT-PCR analysis

In order to test the skin moisturizing ability of Examples 1 to 3 and Comparative Examples 1 and 2, the degree of filla green expression was measured.

The cell lines used in the experiments were the Dr. As the human kerationocyte HaCaT cell line from Fusenig, the cells were divided into 1 × 10 ⁵ cells in a 60 mm dish and cultured for 1 day. Then, the test substances were treated as shown in the following Table 2 and cultured for 24 hours . Total RNA was extracted from the cells cultured and tested by the above method using a triazole (Trizol, Gibco Laboratories, USA), and the total RNA was extracted from the cells by the method provided in One Step RNA PCR Kit (AMV) (Takara Bio Inc., Japan) RT-PCR (reverse transcriptional polymerase chain reaction) was performed. Primers for filaggrin (Bioneer, Korea) were used as primers and beta-actin was used as an internal control for comparison. . After the image analysis, the control group was set at 100, and the comparative values are shown in Table 2 below.

Test substance Concentration used Pillared green production (%) Untreated control group - 100 Comparative Example 1 0.05 ppm 110 0.1 ppm 116 Comparative Example 2 0.05 ppm 115 0.1 ppm 126 Example 1 0.05 ppm 120 0.1 ppm 162 Example 2 0.05 ppm 118 0.1 ppm 157 Example 3 0.05 ppm 116 0.1 ppm 156

As shown in Table 2, it was confirmed that Examples 1 to 3 of the present invention promote the expression of pilar green in the keratinocytes, and the expression of pilar green is remarkably accelerated compared with Comparative Examples 1 and 2 Respectively. Therefore, it can be seen that the composition of the present invention has an effect of promoting the production of natural moisturizing factors of the skin and enhancing the skin moisturizing ability.

[Test Example 3] Measurement of skin moisturizing effect

In order to measure the effect of the ginseng polysaccharide and the green tea polysaccharide on the skin moisturizing effect, Formulation Examples 1 to 4, which are external preparations for nutritional cream formulations, were prepared according to the composition ratios shown in Table 3 below using Comparative Examples 1 to 2 and Examples 1 to 3, 3 and Comparative Formulation Examples 1 to 3 were prepared. The unit of the content ratio of the following ingredients is% by weight.

Compounding ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 0.1 - - - - - Example 2 - 0.1 - - - - Example 3 - - 0.1 - - - Comparative Example 1 - - - 0.1 - - Comparative Example 2 - - - - 0.1 - Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 penta stearate + behenyl alcohol +
Sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol + arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl alcohol + cetearyl glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate + glycerol oleate + propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 6.00 Preservative, incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

In order to confirm the skin moisturizing effect of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 shown in Table 3, the following evaluation was made. Eighty healthy women in their thirties were divided into eight groups each consisting of 10 individuals and applied to the face for 4 weeks for 2 weeks each day under the conditions of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 at a temperature of 24 to 26 DEG C and a humidity of 75% (24 ° C, relative humidity: 40%) after 2 weeks (total 6 weeks) after application, 1 week, 2 weeks and 4 weeks after application and before application, The skin moisture content was measured with a coneometer. The increment of the measured value at each measurement is expressed as a percentage based on the coneometer value measured just before the start of the test, and the results are shown in Table 4 below.

Clinical outcome Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Formulation Example 1 31 39 40 28 Formulation Example 2 32 39 40 28 Formulation Example 3 31 38 39 27 Comparative Formulation Example 1 30 32 32 18 Comparative Formulation Example 2 30 33 34 21 Comparative Formulation Example 3 28 30 29 12

As shown in Table 4, Formulation Examples 1 to 3 including the ginseng polysaccharide and the green tea polysaccharide of the present invention were found to have a significantly increased skin moisture content than those of Comparative Formulations 1 to 3. In addition, the values after 6 weeks after two weeks after stopping the application of the test substance were similar to those after 1 week to 2 weeks after the test substance was applied, and even when the test substance was not applied, It was confirmed that the moisture retention amount of the skin was continuously maintained for a certain period of time. Therefore, it has been found that the composition of the present invention enhances the skin moisturizing ability.

From the above results, it was confirmed that the effect of the ginseng polysaccharide and the green tea polysaccharide was excellent in promoting the differentiation of keratinocytes, restoring damaged skin barrier, and increasing skin moisturizing power.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin and filling them with 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules. And the tablet was tableted using a tablet machine.

[Formulation Example 3] Granules

150 mg of the ginseng polysaccharide of Comparative Example 1, 150 mg of the green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of the red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C to form granules And filled in the next bubble. The final weight of the contents was 1 g.

[Formulation Example 4] Softening lotion (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 5 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Nutritional lotion (milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 6 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.5 The green tea polysaccharide of Comparative Example 2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Nourishing cream

Nutritive creams were prepared according to the compositions listed in Table 7 below in a conventional manner.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 1.0 The green tea polysaccharide of Comparative Example 2 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 7] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 8 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 1.5 The green tea polysaccharide of Comparative Example 2 1.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 8] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 9 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 9] ointment

Ointments were prepared in a conventional manner according to the composition shown in Table 10 below.

Compounding ingredient Content (% by weight) The ginseng polysaccharide of Comparative Example 1 0.1 The green tea polysaccharide of Comparative Example 2 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A composition for external application for moisturizing skin containing ginseng polysaccharide and green tea polysaccharide as an active ingredient. [2] The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. A pharmaceutical composition for moisturizing containing ginseng polysaccharide and green tea polysaccharide as an active ingredient. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are contained in an amount of 0.001-10 wt% based on the total weight of the composition. [Claim 5] The pharmaceutical composition according to claim 4, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1.