KR102298804B1 - Composition for moisturizing the skin containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol - Google Patents

Abstract

The present invention relates to a moisturizing composition, and more particularly, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol It relates to a composition excellent in the effect of restoring damaged skin barrier function and increasing skin moisturizing power by containing as an active ingredient.

Description

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyanosyl-sn-glycerol}

The present invention relates to a moisturizing composition, and more particularly, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol It relates to a composition excellent in the effect of restoring damaged skin barrier function and increasing skin moisturizing power by containing as an active ingredient.

The skin plays a very important role as a barrier function that protects the individual from the outside. The barrier function is a protective function that protects against various external stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) and prevents excessive leakage of body moisture through the skin. It can maintain its function only when it is normally formed.

The stratum corneum (horney layer), which is the outermost of the epidermis, is formed from keratinocytes, and consists of keratinocytes that have completed differentiation and a lipid layer surrounding them (Marcelo CL et al, J. Invest . Dermatol . , 80 , pp37-44, 1983).

Keratinocytes are characteristic cells in which basal cells, which continuously proliferate in the lowermost layer of the epidermis, undergo morphological and functional changes in stages, and rise to the surface of the skin. It is eliminated and new keratinocytes take over their functions, and this repetitive series of changes is called “differentiation of epidermal cells” or “keratinization”.

In addition, during the keratinization process, keratinocytes form a stratum corneum while generating natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acids), thereby making the stratum corneum firm and flexible, thereby forming a skin barrier (skin barrier). function as a barrier).

The stratum corneum can easily lose its function due to lifestyle factors such as excessive washing or bathing, environmental factors such as dry air pollutants, and intrinsic diseases such as atopic skin or senile skin. Due to various factors that have increased in recent years, the number of people complaining of dry skin symptoms and various disorders due to them is increasing.

Accordingly, various studies have been conducted to supply moisture from the outside in order to maintain proper skin moisture or to prevent moisture loss from the body, and various moisturizers having the ability to retain moisture have been developed. As a skin moisturizer, it is common to use substances that can increase water retention in the stratum corneum, such as lipid components such as ceramides, essential fatty acids, and lipid complexes (Rawlings AV et al, J. Invest . Dermatol. , 5, pp731- 741, 1994).

However, recently, the harmful factors to the skin are increasing, and the generation and exfoliation rate of the stratum corneum are slowed down due to changes in dietary habits, and the amount of moisturizing factors and lipids in the stratum corneum are decreased due to the deterioration of the function of keratinocytes. There is an increasing trend in the number of people with skin that does not function as a skin barrier. This disruption of the skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis, and psoriasis. In these diseases, symptom relief can be expected with only a conventional moisturizing agent having a water-retaining function, but a fundamental cure is difficult.

On the other hand, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol is known to have anticancer and anti-inflammatory effects ( Cha BJ et al., Glycosyl glycerides from hydrophobic Panax ginseng inhibited NO production in LPS-stimulated RAW264.7 cells,Journal of Ginseng Research (2014), doi:10.1016/j.jgr.2014.10.005), the efficacy of the compound in relation to improvement of other skin conditions has not yet been specifically known.

Korean Patent Publication No. 10-2012-0083943 (published on July 27, 2012)

Cha B-J et al., Glycosyl glycerides from hydrophobic Panax ginseng inhibited NO production in LPS-stimulated RAW264.7 cells, Journal of Ginseng Research (2014), doi:10.1016/j.jgr.2014.10.005

Therefore, the present inventors were studying to find a substance that exhibits excellent skin moisturizing effect, etc., (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl The present invention was completed by confirming that -sn-glycerol was excellent in restoring the damaged skin barrier function and increasing the amount of skin moisture.

Accordingly, an object of the present invention is to contain (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol as an active ingredient to An object of the present invention is to provide a composition having an excellent moisturizing effect and an effect of improving the damaged skin barrier.

In order to achieve the above object, the present invention provides (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyra, which is a compound represented by the following formula (1). It provides a moisturizing composition containing nosyl-sn-glycerol as an active ingredient.

The composition of the present invention not only exhibits an effect of restoring damaged skin barrier function and increasing skin moisturizing power, but also exhibits an excellent skin regeneration effect. Accordingly, a composition containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol for skin moisturizing, skin barrier function As a composition for strengthening and inducing differentiation of keratinocytes in the skin, it can be usefully used to prevent or improve dry skin, atopic dermatitis, contact dermatitis or psoriasis.

The composition according to the present invention comprises (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol ((2S)-1-O -linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol) as an active ingredient can improve skin moisture.

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol used in the present invention has a structure of the following formula (1), The compound has a molecular weight of 778.5.

[Formula 1]

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol of the present invention is a thesis on glycosyl glycerides (Cha BJ et al., Glycosyl glycerides from hydrophobic Panax ginseng inhibited NO production in LPS-stimulated RAW264.7 cells, Journal of Ginseng Research (2014), doi:10.1016/j.jgr.2014.10.005) was isolated by a known method, but is not limited thereto. It may be extracted from plants, may be synthesized according to methods known in the art, and may be used commercially.

The composition according to the present invention contains (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol based on the total weight of the composition from 0.001 to 50% by weight, preferably 0.01 to 30% by weight, more preferably 0.1 to 10% by weight. If the content of the (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol is less than 0.001% by weight, the efficacy by the component , the effect is weak, and when it exceeds 50% by weight, there is a problem in skin safety or formulation.

The moisturizing composition according to the present invention can be used for strengthening the skin barrier function and for inducing differentiation of keratinocytes in the skin, thus preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation. It can be usefully used as a composition for external application to the skin.

In addition, the composition according to the present invention can be used as a composition for skin regeneration because it has excellent ability to increase the ability to restore damaged skin barrier and increase the amount of moisture in the skin.

The composition of the present invention may be formulated as a composition for external application to the skin, particularly a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any dosage form suitable for topical application, for example, a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, suspension, solid, gel, powder, It may be provided in the form of a paste, foam or aerosol composition. Specifically, the composition of the present invention may be provided in the form of a cream, skin, lotion, powder, ointment, spray, or concealer stick. Compositions of such formulations can be prepared according to conventional methods in the art.

In particular, the composition for external application for skin of the present invention is not particularly limited in formulation as a composition for hair or scalp, for example, hair tonic, hair nourishing lotion, scalp treatment, hair treatment, hair shampoo, hair conditioner, hair lotion, or It may be formulated as a treatment for scalp and hair combination.

In addition, the composition according to the present invention may include other components that can give a synergistic effect to the main effect, preferably within a range that does not impair the main effect, in addition to the above substances. In addition, the composition according to the present invention may further comprise a humectant, emollient, ultraviolet absorber, preservative, bactericide, antioxidant, pH adjuster, organic and inorganic pigment, fragrance, cooling agent or limiting agent. The blending amount of the component can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present invention, and the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition .

In addition, the composition of the present invention may be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to pharmaceuticals, it can be formulated for oral or parenteral administration in solid, semi-solid or liquid form by adding an inorganic or organic carrier that is compatible with the active ingredient used in the present invention. , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, or the like.

Formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets, and the like. In addition, formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated by carrying out a conventional method, and in this case, surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commercially available adjuvants can be appropriately used. have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, the route of administration, or the judgment of the prescriber. Determination of an appropriate dose based on these factors is within the level of the skilled artisan, and its daily dose is for example 0.1 mg/kg/day to 100 mg/kg/day, more specifically 5 mg/kg/day to 50 mg/kg It can be, but is not limited to, /work.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

[Reference Example 1] Preparation of (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol for testing the efficacy of the composition of the present invention BJ et al., Glycosyl glycerides from hydrophobic Panax ginseng inhibited NO production in LPS-stimulated RAW264.7 cells, Journal of Ginseng Research (2014), doi:10.1016/j.jgr.2014.10.005] was used after separation.

[Test Example 1] Measurement of keratinocyte differentiation promoting effect

To investigate the differentiation-promoting effect of (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol in keratinocytes, the following As shown, the amount of CE (Cornified Envelop) generated during differentiation of keratinocytes was measured using absorbance.

First, human keratinocytes (HEK; Lonza, NHEK-Neo-Neonatal Normal Human Epidermal Keratinocytes, Pooled) isolated from the epidermis of newborns were placed in a culture flask and attached to the bottom. After treatment with (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol of Reference Example 1 at a concentration of 5 ppm, the cells were It was cultured for 5 days until it grew to about 70-80% of the floor area. At this time, the low calcium (0.03mM) treatment group and the high calcium (1.2mM) treatment group were used as a negative control and a positive control, respectively. Then, the cultured cells were harvested and washed with PBS (Phophate buffered saline), followed by 10 mM concentration of tris-hydrochloric acid buffer (Tris-HCl, containing 2% sodium dodecyl sulfate (SDS) and 20 mM dithiothreitol) pH 7.4) was added to 1 ml, sonication, boiling, and centrifugation were performed, and the precipitate was suspended again in 1 ml of PBS, and absorbance at 340 nm was measured. Separately, a portion of the solution after sonication was taken to measure the protein content, and was used as a standard when evaluating the degree of cell differentiation. The results are shown in Table 1 below.

test substance Differentiation capacity in keratinocytes (%) Low calcium (0.03 mM) solution
(negative control) 100 High calcium (1.2 mM) solution
(positive control) 210 (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 235

As shown in Table 1, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol was treated with keratinization It was confirmed that the cell differentiation promoting effect was excellent.

[Test Example 2] Measurement of skin barrier function recovery effect

Effects of (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol on the restoration of damaged skin barrier function due to skin damage In order to measure , the following experiment was performed. Damage to the skin barrier using the tape stripping method on the upper arms of 10 adult males and females, and applying two groups of Formulation Example 1 and Comparative Formulation Example 1 prepared with the composition of Table 2 below, respectively, for 7 days The degree of recovery of transdermal water loss (TWEL) once a day was measured and compared with a Vapometer (Delfin, Finland). Here, Formulation Example 1 is a composition containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and comparative formulation Example 1 is a vehicle as a negative control. The experimental results are shown in Table 3 below. The results in Table 3 were compared on the basis of 100% of the difference before and after barrier damage before treatment.

Ingredients (% by weight) Formulation Example 1 Comparative Formulation Example 1 Purified water 69 70 propylene glycol 30 30 (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol One -

test group TWEL change (%) before treatment 1 day 2 days 3 days 4 days 5 days 6 days Formulation Example 1 100 124 120 114 113 109 106 Comparative Formulation Example 1 100 121 112 98 70 62 43

As can be seen in Table 3 above, when (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol is treated It can be seen that the amount of transdermal water loss returns to normal at a fast rate and the barrier damage is restored.

[Test Example 3] Collagen biosynthesis promoting effect

The collagen biosynthesis promoting effect of (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol according to the present invention was compared with TGF-β It was measured by comparison. First, fibroblasts (cell line: KF-4109; obtained from Klavosa) were ^{seeded at 10 5} per well in a 24-well plate and cultured until they grow to about 90%. After culturing this in serum-free DMEM medium for 24 hours, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D of Reference Example 1 dissolved in serum-free medium -Galactopyranosyl-sn-glycerol and TGF-β were treated by 10ng/ml, respectively, and CO ₂ Incubated for 24 hours in an incubator. The supernatant was drained and the increase or decrease of procollagen was observed using a procollagen type (I) ELISA kit (procollagen type (I)). The results are shown in Table 4, and the collagen synthesis ability was compared with the untreated group as 100.

test substance Collagen synthesis (%) untreated group 100 TGF-β 183.5 (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 179.5

From the results of Table 4, (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol used in the present invention is high It was confirmed that it exhibited an excellent level of collagen synthesis ability.

This is because the composition containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol according to the present invention is effective for skin regeneration. suggest that it helps.

[Reference Example 2] Formulation Example 2 and Comparative Formulation Example 2

Nutrient creams were prepared in a conventional manner according to the composition of Table 5 below (unit: wt%).

Ingredients Formulation Example 2 Comparative Formulation Example 2 Purified water To 100 To 100 (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.1 - vegetable hydrogenated oil 1.50 1.50 stearic acid 0.60 0.60 glycerol stearate 1.00 1.00 stearyl alcohol 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 Arachidyl Behenyl Alcohol & Arachidyl Glucoside 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerol Olate & Propylene Glycol 1.50 1.50 Caprylic/Carlic Triglycerides 11.00 11.00 Cyclomedicone 6.00 6.00 preservative, fragrance appropriate amount appropriate amount triethanolamine 0.1 0.1

[Test Example 3] Measurement of skin moisturizing power increase effect

In order to measure the effect of (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol on increasing skin moisture, the formulation Example 2 and Comparative Formulation Example 2 were used, and were evaluated as follows.

Twenty adult men and women in their 40s and 50s classified as dry skin were divided into two groups of 10 for two groups of Formulation Example 2 and Comparative Formulation Example 2, respectively, and the nourishing cream was applied to the face twice daily for 4 weeks. Skin moisture meter under constant temperature and humidity conditions (24°C, 40% relative humidity) before application start, after 1 week, 2 weeks, and 4 weeks after application, and after 2 weeks after application stop (total of 6 weeks) (Corneometer CM825, C+K Electronic Co., Germany) was used to measure the amount of skin moisture. The results are shown in Table 6 below. The results in Table 6 show the increase in the measured value after treatment for a certain period of time based on the skin moisture content meter value measured just before the start of the test as a percentage.

test group Moisture increase rate (%) 1 week elapsed 2 weeks passed 4 weeks passed 6 weeks passed Formulation Example 2 32 36 34 32 Comparative Formulation Example 2 30 32 32 15

Looking at the results of Table 6, when Comparative Formulation Example 2 was applied, the moisture increase rate was about 30% until 4 weeks after the application, but after stopping the application, the skin moisture content rapidly decreased, whereas (2S ) In the case of application of Formulation Example 2 containing -1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol, even after stopping application It can be seen that most of the skin moisture increases by more than 30%. Through this, the composition of the present invention containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol has a skin moisturizing effect. can be seen to be excellent.

[Test Example 4] Confirmation of skin wrinkle improvement effect

In order to confirm the wrinkle improvement effect in humans by the composition of the present invention, Formulation Example 2 and Comparative Formulation Example 2 were used.

In order to confirm the wrinkle improvement effect of Formulation Example 2 and Comparative Formulation Example 2, the evaluation was performed as follows. 20 healthy women in their 40s were divided into two groups of 10 for each of the two groups of Formulation Example 2 and Comparative Formulation Example 2, and the nourishing cream was applied to the face once a day for 12 weeks. The state of wrinkles was measured with a skin meter (visiometer, SV600, Courage+Khazaka electronic GmbH, Germany) and image analysis was performed. The results are shown in Table 7 below. The values in Table 7 below represent the average of each parameter value after 12 weeks of application minus the variable value before application.

after 8 weeks of use
Clinical Results R1 R2 R3 R4 R5 Formulation Example 2 0.18 0.17 0.13 0.01 0.01 Comparative Formulation Example 2 0.27 0.26 0.21 0.03 0.03

R1: The difference between the highest and lowest values of the wrinkle contour line

R2: The average of the R1 values after dividing the wrinkle contour line by 5 arbitrarily

R3 : The highest value among R1 values divided by 5

R4: The average value obtained by subtracting the values of each peak and valley from the baseline of the wrinkle contour line

R5: The difference value of each wrinkle contour subtracted from the baseline of the wrinkle contour line

As shown in Table 7, it was found that the external composition of Formulation Example 2 had a very good skin wrinkle improvement effect.

Hereinafter, formulation examples of the composition according to the present invention will be described, but the pharmaceutical composition and the cosmetic composition can be applied in various formulations, which is not intended to limit the present invention, but only to be specifically described.

[Formulation Example 1] lotion

To prepare a lotion according to a conventional method with the composition shown in Table 8 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 2.0 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 2] Nourishing Cream

A nutritional cream is prepared according to a conventional method with the composition shown in Table 9 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 2.0 Polysorbate 60 1.5 Sorbitan Sesquioleate 0.5 PEG 60 hydrogenated castor oil 2.0 liquid paraffin 10.0 squalane 5.0 caprylic/caprac triglyceride 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 3] Massage Cream

A massage cream is prepared according to a conventional method with the composition shown in Table 10 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 1.0 beeswax 10.0 Polysorbate 60 1.5 PEG 60 hydrogenated castor oil 2.0 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglycerides 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 4] Pack

A pack is prepared according to a conventional method with the composition shown in Table 11 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 1.0 polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 5] Gel

A gel is prepared according to a conventional method with the composition shown in Table 12 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.5 Sodium ethylenediamine acetate 0.05 glycerin 5.0 Carboxyvinyl Polymer 0.3 ethanol 5.0 PEG 60 hydrogenated castor oil 0.5 triethanolamine 0.3 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 6] Ointment

An ointment was prepared in a conventional manner with the composition shown in Table 13 below.

content (wt%) (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.1 glycerin 8.0 butylene glycol 4.0 liquid paraffin 15.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 squalane 1.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 beeswax 4.0 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 7] Soft capsule preparation

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.0025 g, vitamin C 0.0025 g, palm oil 2 mg, hydrogenated palm oil 8 mg , 4 mg of yellow wax and 6 mg of lecithin were mixed, and 400 mg per capsule was filled according to a conventional method to prepare soft capsules.

[Formulation Example 8] Tablet

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.0025 g, vitamin C 0.0025 g, glucose 100 mg, starch 96 mg and After mixing 4 mg of magnesium stearate and adding 40 mg of 30% ethanol to form granules, the mixture was dried at 60° C. and compressed into tablets using a tablet press.

[Formulation Example 9] Granules

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 150mg, vitamin C 150mg, glucose 100mg, and starch 600mg and 100 mg of 30% ethanol was added to form granules, dried at 60° C. to form granules, and then filled in a bag. The final weight of the contents was 1 g.

[Formulation Example 10] Drinks

(2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol 0.0025 g, vitamin C 0.0025 g, glucose 10 g, citric acid 2 g and 187.8 g of purified water was mixed and filled in a bottle. The final volume of the contents was 200 ml.

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, these specific descriptions are only preferred embodiments, and it is clear that the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

Claims (6)

For moisturizing containing (2S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-D-galactopyranosyl-sn-glycerol represented by the following formula (1) as an active ingredient cosmetic composition.
[Formula 1]

The cosmetic composition according to claim 1, wherein the active ingredient is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the composition is for enhancing skin barrier function. The cosmetic composition according to claim 1, wherein the composition is for inducing differentiation of skin keratinocytes. The cosmetic composition according to claim 1, wherein the composition is for skin regeneration. delete