KR20140137244A - Feed composite for culturing leptocephalus - Google Patents

Abstract

The present invention relates to a dietary composition suitable to cultivate a larva of a freshwater eel (Leptocephalus), which is allowed to be applied to the cultivation of Leptocephalus in order to secure good artificial seeds (fry), thereby allowing the mass cultivation. According to a preferable embodiment of the present invention, with regard to a dietary composition supplied to Leptocephalus while the Leptocephalus is contained in a cultivation water tank, the dietary composition is manufactured by crushing the eggs of Seriola quinqueradiata which is a species of fish living in the range of the sea current whereby the Leptocephalus moves to the coast; filtering the eggs which have been crushed and removing egg membranes to obtain an egg substance; and mixing multiple vitamins, an eggwhite and a krill extract with the egg substance, followed by performing a quick freeze drying process. Preferably, the activity of protease in the egg substance is reduced by heat treatment, and fish egg toxins such as phytic acid and lectin are removed from the eggs of Seriola quinqueradiata. More preferably, the multi vitamins are added to the egg substance after the heat treatment of the egg substance.

Description

[0001] FEED COMPOSITE FOR CULTURING LEPTOCEPHALUS [0002] FIELD OF THE INVENTION [0003] The present invention relates to a dietary composition suitable for freshwater eel (Leptocephalus)

The present invention relates to a dietary composition suitable for the culture of freshwater eel fish, and more particularly, to a fishery composition for freshwater eel fish which is adapted to the culture of freshwater eel fish to provide good artificial nursery (fry) ≪ / RTI >

In general, the Far East freshwater eels hatch in the deep sea of the Pacific Ocean (estimated to be the western margin of the Mariana Islands in the South Pacific) and migrate westward along the boreal currents in the form of leptosepalus (ie, freshwater eel fish) It travels northward along the Kuroshio Current, and as it reaches the coast, it transforms into an eel (ie freshwater eel) and grows into a river, a river, or a reservoir. It then echoes back to the deep- .

Such freshwater eels are important production fish species, but their seeds are all dependent on natural fry, and freshwater eel culture is a form of reptose saplings that grows into a river, river or reservoir It is a high-value-added industry that captures migratory eel fishes and grows them as sexually harvested fish. However, fluctuations in farming costs are inevitably accompanied by catches of eel fishes (cheerleaders). In recent years, depletion of natural resources This is a situation that is concerned.

For this reason, in recent years, attempts have been made to establish a complete aquaculture system for artificially growing and hatching from a spermatozoa, transforming it into a fry via a serpentine (Leptosepalus) From the fished fish to the breeding, artificial spawning, and artificial hatching of the broodstock. However, since the larvae of artificial hatching are largely killed during the incubation period, they become a major obstacle to the freshwater eel culture have.

Pretreptosepalus immediately after hatching of hatchlings is fed with egg yolk of mother hatchlings as a nutrient source, and when approximately 8 to 9 days have elapsed after hatching, the dietary hatching activity is initiated. However, until now, Since the transformation process from the spawning process to the fry is not known precisely, development of optimal dietary composition for the actual transformed freshwater eel fish is progressing actively.

SUMMARY OF THE INVENTION The present invention has been made in view of the circumstances of the prior art described above, and it is an object of the present invention to provide a method for producing a fish meal, which comprises the step of applying a heat treatment or a predetermined inhibitor to a fish egg content of a fish living in the pathway of freshwater eel- 'A dietary composition suitable for the culture of freshwater eel' s eardrill, which promotes the growth and survivability of 'freshwater eel' reptosaeparose without decreasing the activity of protease and using the egg yolk of shark The purpose is to provide.

To achieve the above object, according to a preferred embodiment of the present invention, there is provided a dietary composition (feedstuff) of freshwater eel which is fed while containing Leptocephalus of freshwater eel in a culture water tank, , Which is a fish species in the range of the ocean current moving to the coast, is crushed and filtered to remove the egg shell, and the resultant vitamins are mixed with eggs egg white and krill shrimp extracts, followed by rapid freeze drying Powdered feed of freshwater eel shark is provided.

Preferably, the content of the protein is reduced by protease activity by heat treatment.

In addition, more preferably, the phytase (Phytic Acid) and lectin (lectin) are removed from the egg.

Advantageously, said multivitamin is added after the heat treatment of said raw contents, taking into account ingredients which are vulnerable to heat.

According to the powdery feed of the freshwater eel's eardrarium fish according to the present invention, fish species inhabiting the range of migrating currents of a corresponding shark (leptosepalus) instead of the yolk of a shark (particularly, a shark fish) It is possible to reduce the cost of the feed.

In addition, by breaking the eggs and filtering them, the contents of the egg shells in the state of removing the egg shells are obtained. By the heat treatment, the activity of the protease is reduced and the ooze poison is removed to improve the survival rate and growth rate of freshwater eel fish (leptosepalus) .

Furthermore, since the multivitamin preparation is allowed to be mixed with the contents after the heat treatment, the adverse effect due to the deficiency of vitamin is excluded and the normal growth and the efficiency of the feed are increased.

Brief Description of the Drawings Fig. 1 is a graph showing the survival rate versus the number of days of rearing of test fishes (eared fishes) by a dietary composition and a control diet for freshwater eel fish (leptosepalus) culture according to a preferred embodiment of the present invention.

 Hereinafter, preferred embodiments of the present invention will be described in detail.

Examples of the subject of the present invention include pre-ectoparasites and pre-ectoplasmic ectoplasm before they are transformed into eel.

In addition, the fish assumed in the present invention is preferably a fish species inhabited by the path of the re-hatching leptosepalus to the coast, that is, in the range of boreal currents and Kuroshio currents. Typically, Possible defenses (Seriola quinqueradiata) are assumed.

Herein, in the present invention, defense is assumed as a representative fish species of the dietary composition, but it is possible to selectively apply if the species is inhabited in the range of the ocean currents in which the pre-fetopocephalus and leptosepalus move.

On the other hand, according to the present invention, eggs obtained from artificially cultivated or naturally-caught freshwater eels may be applied in order to obtain feeds of reptiles (leptosepalus).

Usually, eggs are composed of eggshell membranes and the contents protected by the egg shells. Eggs are oocyte cells after ovarian growth, Oil balls) or egg yolk accumulation is initiated, and then accumulated in the ovaries of the corresponding fish until it is in vitro fertilized.

Therefore, in the present invention, raw eggs obtained by extracting ovaries of natural or cultured defenses (or freshwater eel) are used, or processed in the form of salting, freezing, liquid or powder.

In order to ensure the uniformity and to always produce the same quality of feed, it is necessary to crush the eggs before the preparation of the feed to obtain the contents of the eggs and mix them with other feed ingredients.

That is, the above-mentioned contents of the egg can be obtained by crushing the fish eggs and removing the egg shell by using a predetermined enzyme component or by filtering with a mesh of, for example, 100 mu m grade.

According to the present invention, in the production of the dietary composition (feed) according to the present invention, it is possible to prevent the growth of a freshwater eel (leptosepalus) including a phytic acid or lectin, It is advantageous to remove it.

In addition, according to the present invention, it is preferable to treat the above-mentioned contents with protease activity. In the protease activity reduction treatment, when a composition having a high protein protein content is heated, a solidified product is formed, (Preferably at a temperature of 45 to 70 캜 for about 40 minutes) or a protease inhibitor (for example, an animal whey protein) in order to prevent clotting, Or an animal plasma (plasma), etc.) is added.

According to the present invention, a feed (dietary composition) is prepared by mixing multivitamin, egg white (or egg white powder), and an additive such as krill shrimp extract to the above-mentioned ingredients.

Here, the above vitamins are essential elements for the health maintenance and improvement of the production activities of the aquaculture fishes, but the environment is difficult to eat in the aquaculture tank, so proper payment is indispensable.

Therefore, in the present invention, the multivitamin includes vitamin B1 (Thiamin) which causes symptoms such as weight loss, growth retardation and mortality due to neuropathy at the time of deficiency, vitamins that cause blindness and photophobia B2 (Riboflavin), Vitamin B6 (Pyridoxine), which plays an important role in protein metabolism in the body and induces abnormal swimming activity, dyspnea and mortality such as increased mortality, rejection of feed intake during shortage, Pantothenic acid is a deficiency symptom. Niacin, which induces a decrease in intake and a decrease in feed efficiency, and a color change in black body, skin lesion in deficiency, muscle atrophy, colon lesion, intake (Biotin) to prevent the onset of symptoms such as depression, cramping and the like.

In addition, the multivitamins include folacin, which causes anemia due to erythrocyte immaturity in the absence of eel or lack of growth, vitamin B12 (cyanocobalamine) required for normal growth and erythrocyte maturation and healthy nerve tissue maintenance, , Inadequate growth rate, anemia, and fin necrosis, and inositol (inositol), which is added in an increased amount when the pre-gelatinized starch is high.

In addition, when the body lacks a methyl source such as methionine or betaine, it also contains choline, which is essential for fat metabolism.

In addition, the above-mentioned multivitamins also include vitamin C, which plays an important role in formation of normal teeth and bones, restoration of bone damage, wound healing, and formation of collagen and cartilage. In case of abnormal production or deficiency of collagen, This causes not only the so-called "lordosis" or scoliosis phenomenon that bend or bend to the left and right, but also the normal formation of the gill gouge is inhibited or the shape of the snout or jaw is deformed.

In addition, the multivitamin includes fat-soluble vitamin A (A1, A2), and vitamin A plays an important role in visual action,

In addition, the multivitamin has biologically active and necessary for the normal formation of the growth skeleton. It also contains vitamin D (D2, D3), and it responds to deficiency of muscular dysfunction, reproductive organ function and corneal peroxide damage Vitamin K, which is necessary for the maintenance of normal blood coagulation rate, which is important for fish in the vitamin E. water, which is caused by a decrease in the ability of red blood cells and severe anemia.

Preferably, according to the present invention, the above-mentioned multivitamins also contain elements that are vulnerable to heat. Therefore, when the corresponding feed is prepared, it is preferable to carry out heat treatment to reduce the activity of the protease in the above- .

In addition, since the multivitamin contains a component susceptible to moisture, it is preferable to freeze and dry the powder during the production of the corresponding feed to allow the feed to be made within 2 to 5 days from the date of manufacture.

According to the present invention, the dietary composition (feed) is added in an amount of 5 to 10% by weight, preferably 6 to 8% by weight, of the multivitamin to 100% Preferably 15 to 20% by weight, and egg white of the egg is added in an amount of 5 to 25% by weight, preferably 10 to 15% by weight.

Therefore, in order to test the growth / survival of the test fish on the dietary composition (feed) according to the present invention, 75% of distilled water was mixed with 25% of the dietary composition by stirring with a stirrer to make 'Feed 1' The feed for the dietary composition (shrimp) was prepared by mixing 51% shark egg, 5% krill shrimp, and 44% distilled water with shark egg as the main ingredient.

For example, 300 horses (Leptosepalus), for example, 7 days old, which were hatched from cultured broodstock, were dispersed in three 10 L type water tanks for breeding with the above-mentioned "Feed 1" and "Feed 2".

The feed 1 and feed 2 were set three times a day from day 8 after the hatching (i.e., starting date of feeding), and the water temperature was set at 24 占 폚. And survival rate were measured to evaluate growth and growth.

As shown in FIG. 1, the test fish of the aquaculture tank fed with 'Feed 1' according to the present invention did not show a sharp decrease, and the survival rate at day 12 from the day of rearing was 95% 80%, respectively.

This tendency was maintained until 20 days after the start of rearing, and the survival rate was 40%, while the survival rate was higher than 31% of 'Feed 2'.

In the case of the test fish in the aquaculture tank fed with feed 1 according to the present invention, it was possible to survive until the transformation into the eel after 20 days from the start of rearing.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.

Claims (3)

In a dietary composition (feed) of a freshwater eel fed with a freshwater eel's Leptocephalus in a aquaculture tank,
The dietary composition is prepared by crushing and deflating defensive eggs as a fish species living in the range of the ocean currents migrating to the coast, and removing the egg shell, and then adding the multivitamin, eggs egg white and krill shrimp extract And then rapidly freeze-dried to obtain a powdery feed of fresh-caught eel. The method according to claim 1, wherein the content of the protein is reduced by protease activity by heat treatment,
Wherein the fish eggs of the fish eggs are removed from the fish eggs of the above-mentioned fish eggs, and phytate (Phytic Acid) and lectins are removed from the fish eggs. The powdery feed of freshwater eel's eardrops according to any one of claims 1 and 2, wherein the multivitamin is added after the heating treatment.

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