KR20140121668A - Natural and bio-functional cosmetic agents comprising black rice bran extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of black rice bran and, more specifically, to a natural functional cosmetic composition for anti-aging, whitening, and reducing wrinkles using an anti-oxidation effect, a melanin synthesis inhibitory effect, and a wrinkle formation inhibitory effect of the black rice bran obtained from polished black rice.

Description

Natural and functional cosmetic compositions comprising black rice extract as active ingredients

The present invention relates to a cosmetic composition containing a black rice extract. More specifically, the present invention relates to a cosmetic composition containing black rice extract, which comprises an antioxidative effect, a melanin formation inhibitory effect and a wrinkle inhibitory effect of skin of black rice bran obtained from black rice, Wrinkle-improving natural functional cosmetic composition.

Skin aging can be classified into aging due to internal factors and aging due to extrinsic factors, resulting in structural and functional changes of the skin depending on the factors. Since the skin is always exposed to the external environment, much of the skin changes due to aging are externally influenced by ultraviolet rays. As the aging progresses, the skin becomes dry, the skin color becomes darker, and the elasticity is lost and wrinkles occur.

One of the main causes of aging, oxygen free radicals, occurs inevitably in metabolic processes that require oxygen. Active oxygen is present in a very unstable state, attacking the tissues in the body, oxidizing and damaging the cells to cause aging.

Active oxygen can oxidize proteins in the body or oxidize the lipids of the cell membrane, which can result in decreased human function, damage DNA, and cause mutations or cancer. In addition, collagen (collagen) gives elasticity to the skin and skin fibrous substance, and aging of the skin advances, and the skin is sagged and sagged and wrinkles are generated.

There is a defensive enzyme system in the body that removes excess active oxygen like superoxide dismutase (SOD). Up to 20 generations, the defense system of the antioxidant enzyme of the human body is active so that it can protect the body healthily. However, As the function of the enzyme is weakened, the human body is more easily exposed to active oxygen and the progress of aging is accelerated, so proper removal of active oxygen for anti-aging is necessary.

The tissue changes caused by aging may vary depending on the structure of the skin, but generally the thickness of the epidermis is thinned, the boundary between the epidermis and the dermis is flattened, and the number of elastic fibers is decreased, resulting in wrinkles.

In addition, the wrinkling of the skin is fundamentally related to changes in the collagen and elastin of the dermal layer as the skin ages due to age or ultraviolet rays. When collagen and elastin of the skin are reduced, elasticity of the skin is reduced and wrinkles are formed.

Melanin in the body protects against cytotoxic substances such as amines, free radicals, and metal ions, but in overproduction forms pigmentation such as spots and freckles and promotes skin aging.

Melanin is a polymeric substance of phenols widely distributed in nature and is a complex of black pigment and protein. Melanin starts from an amino acid called tyrosine in the melanocyte and generates dopa (DOPA). When melanin is oxidized again to generate dopaquinone (DOPA-quinone), the oxidase enzyme tyrosinase ). It then undergoes an autoxidation reaction to produce dark brown melanin. Therefore, whitening cosmetics can anticipate the whitening effect by inhibiting the production of melanin through inhibition of enzyme activity of tyrosinase.

Black rice, which is a type of colored rice, contains anthocyanin, which is a natural coloring ingredient, more than four times as much as black beans. It contains phenolic acids, γ-oryzanol, tocopherol, Tocotrienol, ferulic acid, and other antioxidants. It is rich in minerals such as vitamins, selenium, and iron, which are five times more than ordinary rice. Black rice has been reported to have various physiological activities such as anticancer, antimutagenic, lipid oxidation inhibition. The black rice of Korea has been cultivated for the first time in the Jisan area of Jindo County, and the cultivation area has been gradually spreading. The production volume is steadily increasing, and recently, the development of the improved species is being actively carried out.

Conventionally, black rice has been known as a health food and has been mainly applied to health functional foods. Korean Patent Application Laid-Open No. 10-2004-0067375 and Korean Patent Laid-Open No. 10-2005-0075516 have applied some examples of black rice extract as a cosmetic composition, Was inadequate to show the effective effect.

Accordingly, the present invention provides a multi-functional cosmetic composition for anti-aging, whitening, and wrinkle improvement using a natural extract having inhibition of active oxygen causing aging of skin and inhibiting skin melanin pigment formation and wrinkle improvement and having little cytotoxicity It aims to manufacture.

The present invention relates to a natural multifunctional cosmetic composition having anti-aging, whitening and wrinkle-reducing effects using an extract of black rice, which mainly contains an active ingredient of an antioxidative activity of black rice.

It is an object of the present invention to provide a black rice-extracted multi-functional cosmetic composition comprising a natural extract having excellent anti-aging, whitening and wrinkle-reducing effects and having no skin irritation as an active ingredient.

In order to achieve the above object, the present invention is applied to an effective ingredient of black rice extract as a composition of anti-aging, whitening and wrinkle-improving multi-functional cosmetics of the present invention.

One aspect of the present invention relates to a natural functional cosmetic composition containing a black rice extract as an active ingredient.

Another aspect of the present invention is a natural functional cosmetic composition comprising a natural functional cosmetic composition according to various embodiments of the present invention. The natural functional cosmetic composition may be used as a cleansing cream, a massage cream, a nutritional cream, a sleeping cream, a cleansing gel, a massage gel, a skin, Quot; natural cosmetic < / RTI >

In the present invention, the active ingredient activity of black rice was confirmed to be the highest by comparing the activity of the active ingredients of black rice, black rice, and rice black separated from black rice.

Therefore, the present invention relates to the production of a multi-functional cosmetic composition by extracting only the black rice having the highest activity of the active ingredient in black rice using an extractor and extracting it with an extraction solvent.

The present invention can be manufactured as cosmetics of any formulation conventionally produced in the art as a multifunctional cosmetic composition of black rice extract.

Figure 1 is a graph showing total phenolic acid content changes with extraction time and extraction temperature.
Fig. 2 is a graph showing the antioxidative effect according to the extraction time and extraction temperature.
3 is a graph showing the whitening effect according to the extraction time and extraction temperature.
4 is a graph showing the wrinkle-improving effect according to the extraction time and the extraction temperature.

Hereinafter, various aspects and various embodiments of the present invention will be described in more detail.

In one aspect of the present invention, a natural functional cosmetic composition containing a black rice extract as an active ingredient is disclosed.

In one embodiment, a natural functional cosmetic composition for antioxidation containing a black rice extract as an active ingredient is disclosed.

In another embodiment, the black rice crude extract is obtained by mixing black rice crude powder and an extract solution at a weight ratio of 1: 1 to 20; The extraction solution is obtained by mixing an organic solvent and water in a volume ratio of 30-70: 70-30; Wherein the organic solvent is selected from acetone, ethanol, methanol, and a mixed solution of two or more thereof.

In another embodiment, the natural functional cosmetic composition for antioxidation is characterized in that the extraction is carried out at 20-30 DEG C for 5-7 hours.

When the extraction solution is out of the above range, not only the antioxidative effect is significantly lowered but also the stability of cosmetic antioxidant activity due to interaction with various additives for the production of cosmetics when manufactured into a conventional cosmetic formulation such as cream, lotion or skin Was significantly decreased.

In one embodiment, a natural functional cosmetic composition for whitening comprising a black rice extract as an active ingredient is disclosed.

In another embodiment, the black rice crude extract is obtained by mixing black rice crude powder and an extract solution at a weight ratio of 1: 1 to 20; Wherein the extraction solution is selected from a mixed solution of 100% methanol, 100% acetone, ethanol and water in a volume ratio of 30-70: 70-30.

When the extraction solution is out of the above range, not only the whitening effect is significantly lowered but also when the cosmetic preparation is made into a conventional cosmetic formulation such as cream, lotion or skin, It was confirmed that the uniformity of the whitening action was greatly reduced.

In another embodiment, the natural functional cosmetic composition for whitening is characterized in that the extraction is carried out at 50-70 DEG C for 1-3 hours.

In one embodiment, a natural functional cosmetic composition for improving wrinkles containing a black rice extract as an active ingredient is disclosed.

In another embodiment, the black rice crude extract is obtained by mixing black rice crude powder and an extract solution at a weight ratio of 1: 1 to 20; The extraction solution is obtained by mixing an organic solvent and water in a volume ratio of 30-70: 70-30; Wherein the organic solvent is selected from acetone, ethanol, methanol, and a mixed solution of two or more thereof.

When the extraction solution is out of the above range, not only the wrinkle-reducing effect is significantly lowered but also when the cosmetic preparation is made into a conventional cosmetic formulation such as cream, lotion or skin, It was confirmed that not only the uniformity of the wrinkle-reducing action greatly decreased but also the persistence of the cosmetic wrinkle-reducing action was significantly deteriorated by the interaction with various additives for the production of cosmetics.

In another embodiment, the natural functional cosmetic composition for wrinkle improvement is characterized in that the extraction is carried out at 20-30 DEG C for 1-3 hours.

In the various embodiments, natural functional cosmetic compositions are disclosed, wherein the black rice is obtained by punching black rice paddy rice.

In another embodiment, the present invention is directed to a natural functional cosmetic composition characterized in that the black rice is obtained by turning black rice paddy rice using a vertical grinding mill for 1 to 5 minutes.

According to another aspect of the present invention, there is provided a natural functional cosmetic composition comprising the natural functional cosmetic composition according to any one of claims 1 to 12, wherein the natural functional cosmetic composition comprises a cleansing cream, a massage cream, a nutritional cream, Cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cosmetics, cleansing gels, massage gels, skins and lotions.

According to one embodiment of the present invention, a natural functional cosmetic composition for antioxidation containing a black rice extract as an active ingredient is disclosed. According to another embodiment of the present invention, there is disclosed a natural functional cosmetic composition for whitening comprising a black rice extract as an active ingredient. According to another embodiment of the present invention, a natural functional cosmetic composition for improving wrinkles containing a black rice extract as an active ingredient is disclosed.

Hereinafter, the present invention will be described in more detail with reference to Examples and the like, but the scope and content of the present invention can not be construed to be limited or limited by the following Examples. In addition, it is apparent that, based on the teachings of the present invention including the following examples, those skilled in the art can easily carry out the present invention in which experimental results are not specifically shown.

Example

Production Example 1

In order to prepare the black rice crude extract, black rice (black rice paddy) is first obtained by using a vertical grinding type grinder. Black rice is separated into black rice and black rice (black rice). The degree of the sagging was set to 2 minutes and 40 seconds.

As shown in Table 1 above, when 100 g of black rice was set at a cooking time of 2 minutes and 40 seconds, an average of 82.33 g of black rice was obtained and an average of 17.66 g of black rice was obtained.

Production Example 2

The black rice obtained in Preparation Example 1 was ground using a pulverizer and passed through a sieve body having a sieve size of 300 mu m or less to be pulverized.

Comparative Production Example 1-3

The black rice (Comparative Production Example 1), the black rice (Comparative Production Example 2) and the white rice bran (Comparative Production Example 3) obtained in Production Example 1 were pulverized in the same manner as in Production Example 2 described above.

Example 1

The black rice powder obtained in Preparation Example 2 and 80% methanol were mixed at a weight ratio of 1:10, and the mixture was stirred and extracted twice at room temperature for 2 hours. The obtained extract was filtered with a filter paper to prepare a final extract.

Comparative Example 1-3

The extraction solvent and the extraction time were performed in the same manner as in Example 1 except for using the black rice (Comparative Example 1), the black rice (the portion where the rice bran was removed, the Comparative Example 2), and the white rice bran (Comparative Example 3) The extract was prepared.

Test examples 1-4

The extract obtained in Example 1 was subjected to a comparative experiment of total phenolic acid content, antioxidant activity (DPPH radical scavenging activity), whitening effect (tyrosinase enzyme inhibiting activity) and wrinkle improving effect (enzyme activity inhibitory activity).

(1) Total phenolic acid content was measured by Folin-Ciocalteu method. Specifically, Folin-Ciocalteu coloring reagent (Sigma Co., Ltd, USA) was added to the black rice extract obtained in Example 3, stirred well, and after 3 minutes, 10% Na ₂ CO ₃ was added and reacted in a dark room for 2 hours The absorbance was measured at 700 nm using a UV-visible spectrometer. This is shown in Table 2 below.

(2) Antioxidant activity was measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH). DPPH was purchased from Sigma Co. (Sigma Co., Ltd, USA) and used. For the detailed description of the experiment, first, a standard 7.2 mM DPPH solution was prepared, and the black rice extract obtained in Example 3 was added thereto. After stirring well at room temperature for 30 minutes in a dark room, a UV-visible spectrometer Absorbance was measured at 520 nm. This is shown in Table 3 below.

(3) The inhibitory activity of tyrosinase enzyme activity was measured to determine whitening effect. Specifically, L-DOPA (Sigma Co., Ltd, Korea) and tyrosinase (Sigma Co., Ltd, USA) were added as a substrate to the black rice extract obtained in Example 3, After the reaction, the absorbance was measured at 490 nm using a UV-visible spectrometer. The absorbance of the _control group was defined as A _control and the absorbance of the black rice extract was defined as A _sample . The absorbance was calculated according to the following equation (1).

[Equation 1]

Tyrosinase inhibitory activity = (1-A _sample / A _control ) x 100

(4) The enzyme inhibitory activity of elastase was measured to determine the wrinkle-improving effect. Specifically, Succ-Ala-Ala-Ala-p-nitroanilide was added as a substrate to the black rice extract obtained in Example 3 so as to have a pH of 8.0, stirred well, And the amount of p-nitroanilide produced was measured at 410 nm using a UV-visible spectrometer. The absorbance of the _control group was defined as A _control , and the absorbance of the black rice extract was defined as A _sample . The absorbance was calculated according to the following equation (2).

&Quot; (2) "

Elastase inhibitory activity = (1-A _sample / A _control ) x 100

Comparative Test Examples 1-4

The test was carried out in the same manner as in Test Example 1-4, except that the black rice, the black rice (the portion in which rice bran was removed) and the white rice bran obtained in Comparative Example 1-3 were used as a comparative group. These are shown together in Tables 2-5 below.

division Example 1
(Black rice river) Comparative Example 1
(Black rice) Comparative Example 2
(Black rice) Comparative Example 3
(Rice white rice) mg GAE / g 12.17 + - 0.56 2.75 ± 0.05 0.18 ± 0.05 2.15 + 0.02

As shown in Table 2, the total phenolic acid content of Example 3 (black rice extract) was significantly higher than that of Comparative Example 1-3 of the same concentration. It can be confirmed that the content of phenolic acid is low in Comparative Example 2 in which black rice is removed (black rice, green rice).

division Example 1
(Black rice river) Comparative Example 1
(Black rice) Comparative Example 2
(Black rice) Comparative Example 3
(Rice white rice) mM Trolox / g 36.27 ± 0.27 8.39 ± 0.32 0.14 + 0.33 14.91 + - 0.38

As shown in Table 3, it was confirmed that the antioxidative activity (DPPH radical scavenging activity) of Example 3 (black rice extract) was significantly higher than that of Comparative Example 1-3 at the same concentration. It can be seen that the antioxidant activity of Comparative Example 2 in which black rice was removed (the portion of black rice, the portion in which rice bran was removed) showed almost no antioxidative activity.

division Example 1
(Black rice river) Comparative Example 1
(Black rice) Comparative Example 2
(Black rice) Comparative Example 3
(Rice white rice) Tyrosinase Inhibition (%) 25.83 + - 0.43 14.89 ± 0.43 4.47 ± 0.34 10.08 + - 0.60

division Example 1
(Black rice river) Comparative Example 1
(Black rice) Comparative Example 2
(Black rice) Comparative Example 3
(Rice white rice) Elastase inhibition (%) 13.45 + - 2.42 11.73 + - 2.57 7.14 ± 3.47 9.05 0.27

As shown in Table 4-5, the whitening effect and wrinkle-improving effect of Example 1 are significantly higher than those of Comparative Example 1-3.

Example 2

(50% acetone, 50% ethanol, 50% methanol, 100% acetone, 100% ethanol, 100% methanol, DW) with the extraction solvent (DPPH radical scavenging ability), whitening effect (tyrosinase enzyme inhibiting ability), and wrinkle improving effect (enzyme inhibiting activity of elastase). The test was carried out in the same manner as in Test Example 1-4, and the results are shown in Tables 6-9.

As shown in Table 6, it can be seen that extracts having a higher total phenolic acid content were obtained by extraction using D.W and 50% extraction solvent than 100% extraction solvent. In addition, extraction with a 50% solvent rather than D.W gave an extract having a high total phenolic acid content.

As shown in Table 7, it can be seen that the extract having a higher antioxidative activity (DPPH radical scavenging activity) was obtained by extraction using D.W and 50% extraction solvent than 100% extraction solvent except for methanol. In addition, extraction with a 50% solvent rather than D.W gave an extract having a high total phenolic acid content.

As shown in Table 8, it was possible to obtain an extract having high tyrosinase inhibitory activity (whitening effect) using 100% methanol, 100% acetone and 50% ethanol, And the extraction efficiency of the extract having higher activity than the extraction is high.

As shown in Table 9, it was possible to obtain extracts with high inhibitory effect on elastase enzyme in the order of 50% methanol, 50% ethanol and 100% ethanol, and the extracts using the extraction solvent were more effective than the extracts using the DW The extraction efficiency is high.

Example 3

(25 ° C, 60 ° C), hourly (1 hour, 2 hours, 4 hours, 6 hours) using 50% ethanol selected from Example 1, Were compared with each other. The experiment was carried out in the same manner as in Test Example 1-4, and is shown in Figs.

As shown in FIG. 1, as the extraction time increased, the total phenolic acid content increased at 25 ° C., but decreased at 60 ° C. after 2 hours.

As shown in FIG. 2, the antioxidative activity was increased at 25 ° C. as the extraction time increased. On the contrary, as the extraction time increased at 60 ° C., the antioxidative activity decreased after 1 hour have.

In addition, as shown in FIG. 3, it was found that the tyrosinase inhibitory activity was increased at 25 ° C as the extraction time increased, whereas at 60 ° C, the tyrosinase enzyme inhibitory activity decreased after 2 hours .

In addition, as shown in FIG. 4, as the extraction time increased, the enzyme inhibitory activity at 25 ° C decreased after 2 hours, but did not change significantly after 4 hours. In contrast, at 60 ° C, the enzyme inhibitory activity was decreased after 2 hours.

Example 4

The content of anthocyanins extracted with 50% ethanol was compared with that of black rice, which had the highest activity, by the same procedure as in Example 3 above. This is shown in Table 10 below.

As shown in Table 10, the total anthocyanin content is affected by the extraction temperature and extraction time, and the total anthocyanin content is increased up to 6 hours at 25 ° C., whereas in the case of 60 ° C., Hour, but decreased after 4 hours. It is also found that the extraction of 2 hours at 60 ° C rather than the extraction at 25 ° C results in a higher total anthocyanin content.

Claims (13)

A natural functional cosmetic composition comprising black rice extract as an active ingredient. A natural functional cosmetic composition for antioxidation containing black rice extract as an active ingredient. 3. The black rice extract of claim 2, wherein the black rice crude powder and the extract solution are mixed and extracted at a weight ratio of 1: 1 to 20;
The extraction solution is obtained by mixing an organic solvent and water in a volume ratio of 30-70: 70-30;
Wherein the organic solvent is selected from acetone, ethanol, methanol, and a mixture of two or more thereof. 4. The natural functional cosmetic composition for antioxidation according to claim 3, wherein the extraction is carried out at 20-30 DEG C for 5-7 hours. A natural functional cosmetic composition for whitening, comprising black rice extract as an active ingredient. [Claim 6] The black rice crude extract of claim 5, wherein the black rice crude powder and the extract solution are mixed and extracted at a weight ratio of 1: 1 to 20;
Wherein the extraction solution is selected from a mixed solution of 100% methanol, 100% acetone, ethanol and water in a volume ratio of 30-70: 70-30. 7. The functional cosmetic composition for whitening according to claim 6, wherein the extraction is carried out at 50-70 DEG C for 1-3 hours. A natural functional cosmetic composition for improving wrinkles containing black rice extract as an active ingredient. 9. The black rice extract of claim 8, wherein the black rice crude powder and the extract solution are mixed and extracted at a weight ratio of 1: 1 to 20;
The extraction solution is obtained by mixing an organic solvent and water in a volume ratio of 30-70: 70-30;
Wherein the organic solvent is selected from acetone, ethanol, methanol, and a mixed solution of two or more thereof. 10. The natural functional cosmetic composition for wrinkle improvement according to claim 9, wherein the extraction is carried out at 20-30 DEG C for 1-3 hours. The natural functional cosmetic composition according to claim 1, wherein the black rice is obtained by punching black rice paddy rice. 12. The natural functional cosmetic composition according to claim 11, wherein the black rice is obtained by pouring black rice paddy rice for 1 to 5 minutes using a vertical type grinder. A natural functional cosmetic comprising the natural functional cosmetic composition according to any one of claims 1 to 12,
Wherein said natural functional cosmetics are usable in one of the following applications: cleansing cream, massage cream, nutritional cream, sleeping cream, cleansing gel, massage gel, skin and lotion.

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