KR20140116665A - A composition comprising the seed extract of Rubus coreanus M for preventing and treating skin aging and for skin whitening - Google Patents

Abstract

The present invention relates to a composition comprising a seed extract of Rubus coreanus as an active ingredient and, more specifically, to a pharmaceutical composition for external skin application or a cosmetic composition for skin whitening and preventing and treating skin aging by confirming that the Rubus coreanus seed extract has tyrosinase inhibitory activity, elastase inhibitory activity, cellular tyrosinase inhibitory activity, and pro-collagen biosynthesis inhibitory activity.

Description

FIELD OF THE INVENTION The present invention relates to a composition for preventing and treating skin whitening and skin aging comprising an extract of Rubus coreanus as an active ingredient,

The present invention provides a composition for preventing and treating whitening and skin aging comprising an extract of bokbunja as an active ingredient.

[Document 1] Kligman LH. (1996) The hairless mouse model for photoaging. Clinics in dermatology . 14 (2), 183-195

[Literature 2] Smith, LT, Holbrook, KA, Madri, JA. (1986) Collagen types I, III, and V in human embryonic and fetal skin. Am J Anat . 175 (4), 507-521.

[Literature 3] Chung JH. (2001) Modulation of collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol . 117, 1218-1224.].

[4] Colin R, (2000) Goding Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage. Genes & Dev . 14, 1712-1728.

[Literature 5] Kameyama K, Sakai C, Kuge S, Nishiyama S, Tomita Y, Ito S, Wakamatsu K, Hearing VJ. (1995) Expression of tyrosinase, tyrosinase related protein 1 and 2 (TRP1 and TRP2), silver protein and a melanogenic inhibitor regulates melanogenesis in human melanoma cells. Pigment . Cell . Res . 8, 97-104.

[Literature 6] Roesler WJ, Park EA, McFie PJ. (1998) Characterixation of CCAAT enhancer-binding protein alpha as a cyclic AMP-responsive nuclear regulator. J. Biol . Chem . 273, 14950-14957.

[Literature 7] Rose Busca, Robert Ballotti. (2000) Cyclic AMP a key messenger in the regylation of skin pigmentation. Pigment Cell Res . 13, 60-69.

[Document 8] Hunt G, Todd C, Gresswell JE, Thidy AJ. (1994) α-MSH and its analog Nle ⁴ DPhe ⁷ α-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J. Cell . Sci . 107, 205-211.

[9] Lee NH, Yang HC, Bu HJ, Jung DS, Lee SJ, Riu KZ. (2001) Screening of the tyrosinase inhibitory and hyaluronidase inhibitory activities and radical scavenging effects using plants in Cheju. Kor . J. Pharmacogn . 32 (3), 175-180.

[Literature 10] An BJ, Lee CE, Son JH, Lee JY, Choi GH and Park TS. (2005) Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rhododendron mucronulatum T. J. Korean Soc . Appl . Biol. Chem . 48 (3), 280-284.

[11] Tsuji N, Moriwaki S, Suzuki Y, Takema Y and Imokawa G. (2001) The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem . Photobiol . 74 (2), 283-290.

[Document 12] Kwak YJ, Lee DH, Kim NM and Lee JS. (2005) Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Korean J. Medicinal Crop Sci . 13 (6), 213-216

[Literature 13] Kim MJ, Kim JY, Choi SW, Hong JT and Yoon KS. (2004) Anti-wrinkle effect of safflower ( Cathamus tinctorius ) seed extract. J. Soc . Cosmet . Scientisis Korea . 30 (1), 15-22.

[14] K. C. Sung, (2011) A Study on the Pharmaceutical & Chemical Characteristics and Analysis of Natural Omija Extract, J. of the Korean Oil Chemists' Soc., 28 (3), 290].

[Literature 15] H. M. Yang et al., (2007) A Comparison on the Inflammation Control Efficacy of native Bokbunja and foreign Bokbunja, Kor. j. Food Sci. Technol. Soc., 3, 342.

[16] Carmichael J, DeGraff WG, Gazdar AF, Minna JD and Mitchell JB. (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res . 47 (4), 936-942.

[17] Yagi A, Kanbara T and Morinobu N. (1986) The effect of tyrosinase inhibition for aloe. Planta Media . 3981, 517-519.

[Literature 18] Akiu S, Suzuki Y, Asahara T, Fujinuma Y and Fukuda M. (1991) Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells. Nippon Hifuka Gakkai Zasshi . 101 (6), 609-613

[19] Hosoi J. Abe E, Suda T and Kuroki T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res . 45 (4), 1474-1478

[Document 20] Cannell RJP, Kellan SJ, Owsians AM and Walker JM. (1988) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Media . 54 (1), 10-14

[Literature 21] Kim JE. (2003) Effect of epigallocatechin-3-gallate on heat-modulated type Ⅰprocollagen, MMP-1, and TIMP-1 expression in cultured human dermal fibroblasts. Korea University. Seoul, Korea.

Skin aging is caused by a structural change in the extracellular matrix present in the dermal layer of the skin [Kligman LH. (1996) The hairless mouse model for photoaging. Clinics in dermatology . 14 (2), 183-195.) The denaturation of elastic fibers and the reduction of the amount of collagen in the components cause wrinkles of the skin [Smith, LT, Holbrook, KA, Madri, JA. (1986) Collagen types I, III, and V in human embryonic and fetal skin. Am J Anat . 175 (4), 507-521. Among the major factors leading to skin aging is the generation of active oxygen by ultraviolet rays or stress related to reduction of collagen. In addition, the production of melanin to protect the body from skin damage, aging or cancer caused by UV (ultraviolet) causes various skin diseases when the function of pigment synthesis or distribution is obstructed, Sponges, freckles, and senile spots. In recent years, interest in the aesthetic desires and health of people has been increasing, and there has been a growing interest in the development of functional cosmetics that inhibit the production of melanin [Chung JH. (2001) Modulation of collagen metabolism in aged and photoaged human skin in vivo. J Invest Dermatol . 117, 1218-1224.].

Melanin, a key determinant of skin tone, is biosynthesized in melanosomes in pigmented cells called melanocytes of the epidermal basal layer. The starting material for the synthesis of melanin is tyrosine, an amino acid. Tyrosine is oxidized by tyrosinase in melanocytes to L-3,4-dihydroxyl phenylalanine (DOPA) and DOPA quinone. It is known that DOPA quinone is then converted to DOPA chrome, 5,6-dihydroxyindole, indole-5,6-quinone and then melanin by polymerization with indole-5,6-quinone [Colin R, ) Goding Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage. Genes & Dev . 14, 1712-1728.].

In addition, tyrosinase plays an important role in the formation of melanin in the skin. Tyrosine hydoxylase, which oxidizes tyrosine in melanosome to form DOPA, acts as a DOPA oxidase that oxidizes DOPA to form DOPA quinone. [Kameyama K, Sakai C, Kuge S, Nishiyama S, Tomita Y, Ito S, Wakamatsu K, Hearing VJ. (1995) Expression of tyrosinase, tyrosinase related protein 1 and 2 (TRP1 and TRP2), silver protein and a melanogenic inhibitor regulates melanogenesis in human melanoma cells. Pigment . Cell . Res . 8, 97-104.]

Currently, many studies have been conducted in the medical and cosmetic industries to inhibit the production of melanin excess, and there have been known inhibitors of tyrosinase, such as hydroquinone, hydroxyanisole, Ascorbic acid, kojic acid, and azelaic acid. However, only limited amounts are used for problems such as skin safety and formulation stability [Roesler WJ, Park EA, McFie PJ. (1998) Characterixation of CCAAT enhancer-binding protein alpha as a cyclic AMP-responsive nuclear regulator. J. Biol . Chem . 273, 14950-14957.]. Accordingly, studies have been made to find non-mutagenic whitening agents while reducing cytotoxicity and reducing melanin synthesis, and in particular, natural materials are attracting attention.

Melanin, the most important factor in determining human skin color, not only inhibits skin photoaging and sunsclerosis [Rose Busca, Robert Ballotti. (2000) Cyclic AMP a key messenger in the regylation of skin pigmentation. Pigment Cell Res . 13, 60-69.] It is responsible for the partial hyperpigmentation of spots, freckles, black spots, etc. [Hunt G, Todd C, Gresswell JE, Thidy AJ. (1994) α-MSH and its analog Nle ⁴ DPhe ⁷ α-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J. Cell . Sci . 107, 205-211.) Melanin is biosynthesized in the melanocytes of the melanocytes in the basal layer of the epidermis, and is transferred to peripheral keratinocytes through the dendrites of melanocytes and migrates into the stratum corneum of the skin [Lee, NH, Yang, HC HJ, Jung DS, Lee SJ, Riu KZ. (2001) Screening of the tyrosinase inhibitory and hyaluronidase inhibitory activities and radical scavenging effects using plants in Cheju. Kor . J. Pharmacogn . 32 (3), 175-180.). L-ascorbic acid and its derivatives and kojic acid, arbutin, lactic acid, glucosamine, and tunicamycin have been developed to inhibit melanogenesis. There is a problem in stability and only a very limited amount is used [An BJ, Lee, JH, Lee JY, Choi GH and Park TS. (2005) Antioxidant, anticancer and tyrosinase inhibitory activities of extracts from Rhododendron mucronulatum T. J. Korean Soc . Appl . Biol . Chem . 48 (3), 280-284).

Skin aging can be roughly classified into two types among the aging of the body. The first is the aging phenomenon that can not be avoided due to the aging of the skin, and the second is skin aging caused by the exposure to sunlight as a photoaging and exogenous skin aging [Tsuji N, Moriwaki S, Suzuki Y, Takema Y and Imokawa G. (2001) The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem . Photobiol . 74 (2), 283-290.]. In the dermis of the skin, collagen and elastin form a network structure to maintain the elasticity of the skin. The elasticity and the wettability are reduced due to the internal and external stresses such as age and ultraviolet rays, and the elasticity of elastin When the mesh structure breaks, the skin becomes sagged and wrinkles occur, resulting in skin aging [Kwak YJ, Lee DH, Kim NM and Lee JS. (2005) Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Korean J. Medicinal Crop Sci . 13 (6), 213-216.). Therefore, skin aging can be inhibited by inhibiting the activity of elastase, elastase, which is one of the main causes of skin aging. Therefore, it is possible to inhibit skin aging by inhibiting the activity of elastase, an elastin degrading enzyme, which is one of the main causes of skin aging [Kim MJ, Kim JY, Choi SW, Hong JT and Yoon KS. (2004) Anti-wrinkle effect of safflower ( Cathamus tinctorius ) seed extract. J. Soc . Cosmet . Scientisis Korea . 30 (1), 15-22.).

Rubus coreanus M is known to be effective in the treatment of interpoly, renal, and diuretic effects in a single room. It is known to treat depression, vomiting, and urinary frequency. Carvone acid, vitamin C and polysaccharides Are known. (Information and Communication, pp. 652-654, Young Lim, 1998)

Studies on the physiological activity of bokbunja strawberry have shown that two kinds of flavan-3-ol (ol), one proanthocyanidin and one ellagitanin Separation and separation of phenol compounds such as flavonoids from the leaves were reported, and the physiological activity of the fruits was reported [K. C. Sung, (2011) A Study on the Pharmaceutical & Chemical Characteristics and Analysis of Natural Omija Extract, J. of the Korean Oil Chemists' Soc., 28 (3), 290].

In the meantime, research on the development of processed foods has been carried out, such as the development of liquor using bokbunja, and the preparation of dry noodles using immature bokbunja powder [HM Yang et al., (2007) A Comparison on the Inflammation Control Efficacy of native Bokbunja and foreign Bokbunja, Kor. j. Food Sci. Technol. Soc., 3, 342].

In this study, the whitening and wrinkle - related activities of the brambled seeds were verified as a cosmetic material.

However, it has not been taught or described in the above-mentioned literatures that the bokbunja extract can be used as a composition for whitening and prevention of skin aging and therapeutic effect.

Thus, the present inventors have found that the bramblymus seed extract can be used as a medicament for inhibiting tyrosinase enzyme activity, elastase enzyme inhibition activity, celluli tyrosinase enzyme inhibition activity, melanin biosynthesis inhibiting activity, pro- collagen, and biosynthesis inhibitory activity, the present inventors have completed the present invention by confirming that the composition can be effectively used as an external dermatological pharmaceutical composition or cosmetic composition for prevention and treatment of whitening and skin aging.

In order to achieve the above object, the present invention provides a dermatological pharmaceutical composition for treatment and prevention of whitening and skin aging, which comprises an extract of bokbunja as an active ingredient.

As used herein, "skin aging" includes wrinkles, stains, muscle wounds, skin damage due to ultraviolet rays, skin cancer, and preferably wrinkles.

The extract of the present invention is characterized by containing 0.1 to 50% by weight based on the total weight of the dermatological pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In order to achieve the above object, the present invention provides a cosmetic composition for improving and preventing whitening and skin aging comprising an extract of bokbunja as an active ingredient.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

The bramble seed extract as defined herein is prepared by mixing water, a lower alcohol having 1 to 4 carbon atoms, acetone or a mixed solvent thereof, preferably water, ethanol, acetone or a mixed solvent thereof, more preferably water and an ethanol mixed solvent , Even more preferably from 30 to 90% water and ethanol mixed solvent; Specifically, the washed rice bran seeds are washed and then washed with water, a lower alcohol having 1 to 4 carbon atoms, acetone, or a mixed solvent thereof, preferably water, methanol, ethanol or a mixed solvent thereof, more preferably water and an ethanol mixture More preferably 30 to 90% water and ethanol mixed solvent at a temperature of 0 ° C to 150 ° C, preferably 20 ° C to 100 ° C for 30 minutes to 72 hours, preferably 12 hours to 48 hours The extract obtained by repeatedly performing the ultrasonic extraction method, the hot water extraction method, the room temperature extraction method or the reflux extraction method, preferably the room temperature extraction method, about 1 to 20 times, preferably 2 to 10 times, is subjected to filtration, ≪ / RTI >

The extract prepared by the above-mentioned method can be used as an agent for inhibiting tyrosinase enzyme activity, Elastase enzyme inhibitory activity, celluli tyrosinase enzyme inhibitory activity, melanin biosynthesis inhibitory activity, pro- collagen and biosynthesis inhibitory activity, it was confirmed that the composition is useful as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating whitening and skin aging.

The extract of the present invention is characterized by containing 0.1 to 50% by weight based on the total weight of the dermatological pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

Accordingly, the present invention provides an external dermatological pharmaceutical composition and a cosmetic composition for the treatment and prevention of whitening and skin aging, which comprises the following production method and the extract obtained by the production method as an active ingredient.

In addition, the brambly seed of the present invention has been used for a long period of herbal medicine and food, and the extract of the present invention extracted therefrom has no problems such as toxicity and side effects and has proved to be a non-irritant sample in the skin patch test. Can be used with confidence.

The dermatological pharmaceutical composition containing the extract of the present invention can be used as a pharmaceutical composition in the form of external preparation for skin such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma However, the present invention is not limited thereto.

The preferred dosage of the extract of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at a daily dose of 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The extract of the present invention can be used variously in cosmetics and cleansers having a whitening and anti-aging effect.

Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.

The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

As described above, the brambler seed extract of the present invention is useful as an inhibitor of tyrosinase enzyme activity, an enzyme activity inhibiting activity, a cellulase tyrosinase enzyme inhibition activity, a melanin biosynthesis inhibiting activity, Pro-collagen biosynthesis inhibitory activity and the like, the composition is useful as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating whitening and skin aging.

Brief Description of the Drawings Fig. 1 is a graph showing the cell survival rate of mouse melanoma cell and human fibroblast of bokbunja seed extract (the results are expressed as mean (SD) means ± SD);
Fig. 2 is a graph showing the mushroom tyrosinase inhibitory activity of the bokbunja seed extract (the results are expressed as means (mean) ± SD of triplicate);
Fig. 3 is a graph showing B16F10 cytotoxic inhibitory activity of bokbunja seed extract (the results are expressed as mean (mean) SD of triplicate);
FIG. 4 is a graph showing the effect of bramblymus cereus extract on the amount of B16F10 cell tyrosinase melanin (the results are expressed as means (mean) ± SD of triplicate);
Fig. 5 is a graph showing the inhibitory activity of Rubus coreanus extract on elastase (the results are expressed as mean (mean) SD of triplicate);
Fig. 6 is a graph showing the effect of bokbunja seed extract on pro-collagen biosynthesis to elastase (the results are expressed as mean (mean) SD of triplicate values).

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Reference Examples and Experimental Examples.

Example 1. Preparation of Bokbunja Seed Extract

Dried bramble seeds 100g ( Rubus coreanus M. Seed, Heinlee, Gimcheon City, Gyeongbuk), and the supernatant and precipitate were separated from the supernatant by repeating the same procedure three times. Each extract was centrifuged and filtered (filter paper; Whatman No. 2), concentrated and lyophilized to give 3.52 g of brambly seed ethanol extract. (Hereinafter referred to as RC70E). This was used as a sample of this experiment while being stored in a refrigerator room.

Reference example  1. Experimental preparation

1-1. Whitening and anti-wrinkle effect measuring reagent

The mushroom tyrosinase, a reagent used for measuring whitening and wrinkle suppressing effect, was purchased from Sigma Chemical Co. (St. Louis, Mo., USA), L-3,4-dihydroxyphenyl-alanine Sigma Chemical Co. (St, Louis, MO, USA), L-DOPA), porcine pancreas elastase. (St, Louis, MO, USA), PPE), Aster glehni-succinyl- (L-Ala) 3-Aster glehni-nitroanilide Sigma Chemical Co. (St, Louis, Mo., USA), collagenase Sigma Chemical Co. (St, Louis, MO, USA) and 4-phenylazo benzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg. (St, Louis, MO, USA) were purchased and used.

1-2. Devices used in experiments

The instrument used for the physiological activity experiments was a UV / VIS spectrophotometer (UVD-3200, Hitachi, Japan), a rotary vacuum evaporator (R-215V, Tokyo, Rikakikai Co.), a centri-fuge (Sorvall, Hitachi, Japan) BOD incubator (Brinkmann 632, Metrohm, Switzerland), CO2 incubator (SANYO MCO 20AIC, Hanbaek Scientific Co. Korea), pH meter (Brinkmann 632, Metrohm, Switzerland), microscope (Nikon Eclipse Ti-U, Nikon, Japan) LTI-1000SD, Hanbaek Co. Korea), autoclave (HB-506, Hanbaek Scientific Co. Korea) and ELISA reader (VersaMax, Bio Rad, Japan).

1-3. Cell culture

The cells were cultured in Dulbeco's modified eagle's medium (New York, USA DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin / streptomycin (100 U / , Incubated in a 5% CO2 incubator and subcultured.

Experimental Example  One. MTT assay Of cell survival

To test the cell survival rate by MTT assay of the samples obtained in the examples, the method of Carmichael disclosed in the document [Carmichael J, DeGraff WG, Gazdar AF, Minna JD and Mitchell JB. (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res . 47 (4), 936-942.

Each cell line (melanoma (B16F10, ATCC), fibroblast (CCRF S-180, ATCC), macrophage (Raw 264.7) cell and ATCC) was dispensed in a 96 well plate at a concentration of 0.6 to 8 × 10 3 cells / 0.02 mL was added, and the cells were cultured in a 5% CO 2 incubator at 37 ° C for 24 hours.

In the control group, the same amount of distilled water as that of the sample was added and the cells were cultured under the same conditions. After adding 0.02 mL of the MTT solution prepared at a concentration of 5 mg / mL for 4 hours, the culture solution was removed, and 0.15 mL of DMSO: Ethanol (1: 1) was added to each well and reacted at room temperature for 30 minutes. Absorbance was measured at 550 nm. The cell viability was measured by the absorbance reduction rate of the sample solution addition group and the no addition group.

As a result, cell viability was measured at the concentrations of 1, 10, 25, 50, and 100 μg / ml of the bokbunja extract in mouse melanocytes and human fibroblasts, as shown in FIG. The cell viability of mouse melanocytes was 99, 92, 74, 56 and 42%, and that of fibroblasts was 95, 90, 65, 62, and 58%, respectively, as measured by mouse melanocytes and human fibroblasts %, Respectively. As a result, it was confirmed that brambled seeds showed weak toxicity at 25, 50 and 100 μg / ml in both cells, and the experiments were carried out at concentrations of 1 and 10 μg / ml, which are high cell survival rates.

Experimental Example  2. Whitening activity experiment

1-1. Tyrosinase  Inhibitory activity

In order to test the tyrosinase inhibitory activity of the samples obtained in the examples, Yagi et al. (Yagi A, Kanbara T and Morinobu N. (1986) applied the method of Yagi et al. aloe Planta Media . 3981, 517-519.]

To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA was dissolved in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and 0.1 mL of the sample solution, DOPA chrome produced in the reaction solution was measured at 475 nm for 2 minutes. The tyrosinase inhibitory activity was expressed by the absorbance reduction rate of the sample solution and the non-added sample.

As a result of the present experiment, the result of measurement of tyrosinase inhibitory activity from mushroom to measure tyrosinase inhibitory activity which can effectively inhibit melanin polymer biosynthesis in the skin is shown in FIG. In this experiment, the bacterium seed extract was reacted with mushroom-derived tyrosinase enzyme at a concentration of 10, 100, and 1000 μg / ml, and showed 41%, 49%, and 52%, respectively.

1-2. Intracellular Tyrosinase  Inhibitory activity

In order to test cellular intracellular tyrosinase inhibitory activity of the samples obtained in the examples, the following method was applied as described in the literature (Akiu S, Suzuki Y, Asahara T, Fujinuma Y and Fukuda M. (1991) Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells. Nippon Hifuka Gakkai Zasshi . 101 (6), 609-613).

Melanoma (B16F10 ATCC) cells were filled in each culture dish, and the medium was removed and washed with PBS. 100 μl of lysis buffer (67 mM sodium phosphate buffer, 1% triton X-100, 0.1 mM phenylmethyl sulfonyfluoride) was added to each dish, and the cells were disrupted by being left on ice. Cells were collected and ultrasonicated. The supernatant obtained by centrifugation at 12,500 rpm for 20 minutes was used as an enzyme solution. After adding 120 μL of 8.0 mM L-DOPA dissolved in 67 mM phosphate buffer (pH 6.8) and 40 μL of the sample solution into a 96-well plate, 40 μL of the enzyme solution dissolved in 67 mM phosphate buffer (pH 6.8) After minute incubation, the amount of DOPA chrome produced was measured at 490 nm.

As a result of this experiment, the measurement result of mushroom-derived tyrosinase inhibitory activity is shown in FIG. The brambled seed extract showed an inhibition rate of 61% at 1, 000 μg / ml.

1-3. Melanin ( melanin ) Biosynthesis inhibition rate

In order to test the melanin biosynthesis inhibitory activity of skin melanoma cells of the samples obtained in the examples, a method of measuring the inhibition rate of melanin biosynthesis in Melanoma cell (B16F10) disclosed in Hosoi et al. [Hosoi J. Abe E, Suda T and Kuroki T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res . 45 (4), 1474-1478].

The melanoma cells (B16F10) cultured in DMEM medium were divided into 2 × 10 6 cells / dish in a 100 mm culture dish. After culturing for 24 hours, 2 ml of each sample was prepared and the cells were added with phosphate buffer (pH 7.4 ). After the cells were desorbed with 0.25 M trypsin-EDTA solution, the harvested cells were treated with 1 mL of 5% TCA per 1 × 10 ⁶ cells, centrifuged twice at 2,500 rpm, and the separated melanin was washed with phosphate buffer Then, 1 mL of ether: ethanol (1: 3) was added, and the mixture was centrifuged twice. 1N NaOH was added to the dried melanin, reacted at 80 ° C for 1 hour, and the absorbance was measured at 405 nm by a spectrophotometer. The inhibition of melanin biosynthesis was expressed by the absorbance reduction ratio of the sample solution and the non-added sample.

Melanin biosynthesis in melanoma cells was measured in order to use bokbunja seed extract as a natural whitening agent, as shown in Fig. The extracts of bokbunja extract showed 79 and 42% inhibition of melanin at concentrations of 1 and 10 μg / ml, respectively.

Experimental Example  3. Wrinkle-improving efficacy experiment

3-1. Elastase ( Elastase ) Inhibition activity measurement

In order to test the elastase inhibitory activity of the samples obtained in the examples, Cannell et al. (Cannell RJP, Kellan SJ, Owsians AM and Walker JM. (1988) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Media . 54 (1), 10-14].

N-succinyl- (L-Ala) 3-Aster glehni-nitroanilide was used as a substrate and the amount of Aster glehni-nitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 405 nm. Each test solution was prepared in a test tube at a constant concentration of 0.5 mL. The test tube was washed with porcine pancreas elastase (2.5 U / mL, Sigma Chemical Co., St. Louis, Mo., USA) dissolved in 50 mM tris-HCl buffer (pH 8.6) ) Was added and 0.5 ml of N-succinyl- (L-Ala) 3-Aster glehni-nitroanilide (0.5 mg / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) Respectively. Elastase inhibitory activity was expressed as the absorbance reduction ratio of the sample solution and the non - added sample.

The result of measuring the elastase inhibitory activity associated with wrinkle formation is shown in FIG. In the case of bokbunja, the elastase inhibitory effect was 2%, 14% and 23% at 10, 100 and 1000 μg / ml, respectively. In the control group, EGCG showed 31% elastase inhibition effect at 1000 μg / ml, indicating that the brambled seeds had an inhibitory effect similar to EGCG and thus showed an efficacy against Elstase inhibition.

3-2. Pro-collagen biosynthesis assay

In order to test the effect of the samples obtained in the examples on the pro-collagen biosynthesis, the method described in the literature was applied as follows [Kim JE. (2003) Effect of epigallocatechin-3-gallate on heat-modulated type Ⅰprocollagen, MMP-1, and TIMP-1 expression in cultured human dermal fibroblasts. Korea University. Seoul, Korea].

Procollagen Type-I C-Peptide (PIP) EIA kit was purchased from TAKARA Bio Inc. to measure the amount of pro-collagen type I synthesis when CCD-986sk cells (ATCC) (Shiga, Japan). Cells were plated at a density of 1 × 10 ⁴ cells / well in a 96-well plate and then stabilized for 24 hours. After that, the cultured medium was removed, and the sample extracts were treated at different concentrations, followed by culturing for 48 hours. The supernatant from each well was recovered and added to each well of procollagen Type-I C-Peptide (PIP) EIA kit, and then the total amount of procollagen type I was measured according to the manufacturer's method.

As a result, the amount of collagen biosynthesis measured by procollagen type Ⅰ C-peptide (PICP) enzyme immunoassay on the fibroblasts of the bokbunja seed extract was measured as shown in FIG. In the case of the bokbunja seed extract, the effect was 30, 58% at the concentration of 1, 10 μg / ml, and it was confirmed that the efficacy was similar in comparison with the control group EGCG.

Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.

Formulation Example  1. Cream composition

The oil phase and water phase are heated to 75 ° C and cooled to room temperature.

Formulation Example  2. Massage Cream  Composition

The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.

Formulation Example  3. lotion composition

The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.

Formulation Example  4. Skin lotion composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  5. Essence composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  6. Pack composition

The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.

Formulation Example  7. Cleansing Foam  Composition

The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (6)

A method for the treatment and prevention of skin whitening and skin aging comprising an extract of bokbunja as an active ingredient. [Claim 7] The composition according to claim 1, wherein the skin aging is wrinkle, stain, main muscle, skin damage due to ultraviolet rays, or skin cancer. The dermatological pharmaceutical composition according to claim 1, wherein the brambunial seed extract is an extract extractable in water, a lower alcohol having 1 to 4 carbon atoms, acetone, or a mixed solvent thereof. The dermatological pharmaceutical composition according to claim 1, wherein the dermatological pharmaceutical composition comprises a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma . A cosmetic composition for improving and preventing whitening and aging of skin comprising an extract of bokbunja as an active ingredient. The cosmetic composition according to claim 5, wherein the cosmetic composition is a formulation of lotion, skin, lotion, nutritional lotion, nutritional cream, massage cream, essence, and pack.

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