KR20140110565A - A composition comprising the inner shell extract or purified extract of Castane crenata S. et Z. for preventing and treating skin aging - Google Patents

Abstract

The present invention relates to a composition comprising an extract or a purified product as an active ingredient. More particularly, the extract or purified product of the extract has a DPPH radical scavenging activity, an ABTS radical cation decolorization activity increase, a superoxide dismutase (SOD) -like activity, measurement of xanthine oxidase inhibitory activity, and inhibition of nitrite scavenging activity, the composition can be used as a skin external pharmaceutical composition or cosmetic composition for preventing and treating skin aging .

Description

[0001] The present invention relates to a composition for preventing and treating skin aging which contains an extract of tubercle bark or a purified product as an active ingredient,

The present invention provides a composition for preventing and treating skin aging comprising an extract or a purified product as an active ingredient.

[Document 1] Metcalfe DD, Kaliner M, Donlon MA. The mast cell. Crit. Rev. Immunol. 1981; 3: 23-74.

[2] Metzger H, Alcaraz G, Hohman R, Kinet JP, Pribluda V, Quarto R. The receptor with high affinity for immunoglobulin E. Annu. Rev. Immunol. 1986; 4: 419-70

[Literature 3] Chand N, Pillar J, Diamantis W, Perhach JL, Sophia RD. Inhibition of calcium ionophore (A23187) stimulated histamine release from rat peritoneal mast cells by azelastine: implications for its mode of action. Eur. J. Pharmacol. 1983; 96: 227-33; 11

[4] Takei M, Umeyama A, Shoji N, Arihara S, Endo K. Mechanism of inhibition of IgE-dependent histamine release from rat mast cells by penasterol and penasterone. J. Pharm. Sci. 1995; 84: 228-30

[Literature 5] Galli SJ, Gordon JR, Wershil BK. Cytokine production by mast cells and basophils. Curr. Opin. Immunol. 1991; 3: 865-72

[Literature 6] Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation. Nature 2008; 454: 445-54

[Literature 7] Arend WP, Dayer JM. Inhibition of the production and effect of interleukin-1 and tumor necrosis factor alpha in rheumatoid arthritis. Arthritis Rheum. 1995; 38: 151-60

[Literature 8] Barnes PJ, Adcock I. Anti-inflammatory actions of steroids: molecular mechanisms. Trends Pharmacol. Sci. 1993; 14: 436-41; 25;

[Document 9] Butler DM, Maini RN, Feldmann M, Brennan FM. Modulation of proinflammatory cytokine release in rheumatoid synovial membrane cell cultures. Comparison of monoclonal anti-TNF-a antibody with the IL-1 receptor antagonist. Eur. Cytokine Netw. 1995; 6; 225-30

[Document 10] Erchler WB, Keller ET. Age-associated increased IL-6 gene expression, late-life diseases, and frailty. Annu. Rev. Med. 2000; 51: 245-70

[Document 11] Harada A, Mukaida N, Matsushima K. IL-8 as a novel target for interventional theraphy in acute inflammatory disease. Mol. Med. Today. 1996; 2: 482-9

[12] Galli SJ, Kalesnikoff J, Grimbaldeston MA, Piliponsky AM, Williams CM, Tsai M. Mast cells as "tunable" effector and immunoregulatory cells: recent advances. Annu. Rev. Immunol. 2005; 23: 749-86;

[Literature 13] Azzolina A, Bongiovannia A, Lampiasi N. Substance p induces TNF-α and IL-6 production through NFκB in peritoneal mast cells. Biochim. Biophys. Acta. 2003; 1643: 75-83;

[14] Kim SH, Jun CD, Suk K, Choi BJ, Lim H, Park S, Lee SH, Shin HY, Kim DK, Shin TY. Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol. Sci. 2006; 91: 123-31

[Literature 15] Tan SL, Parker PJ. Emerging and diverse roles of protein kinase C in immune cell signaling. Biochem J. 2003; 376: 545-52

[Document 16] Kim MJ, Lee, Hwang MS, Kim SC, Lee MH. Blooming, fructification and nut characteristics of chestnut cultivars cultivated in korea. Jour. Korean For. Soc 2003; 92: 321-32

[Document 17] Kim CM, Lee JS, An DK, Sim MK. The encyclopedia of China oriental herbal medicine. 1st rev. ed. Seoul: Jungdamsa. 2006. 4372-4373

[Document 18] Moon JS. A study of physicochemical properties of starch separated from chestnut inner shell waste. Dongshin University. 1999

[Literature 19] Blois, MS 1958. Antioxidant determination by the use of a stable free radical. Nature 26 , 1199-1204.

[Literature 20] Roterta, R., P. Nicoletta, P. Anna, P. Ananth, Y. Min and RE Catherine. 1999. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Radic . Biol . Med. 26 , 1231-1237.

[21] Siddhuraju, P., Becker, K. (2007), The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata L.) seed extracts. J. of Food Chemistry. 101: 1019.

[Document 22] Jayaprakasha, GK, RL Jaganmohan and KK Sakariah. 2004. Antioxidant activities of flavin in different in vitro moder systems. Bioorganic & Medicinal Chem . 12 , 5141-5146.

Mast cells are distributed in organs such as skin, respiratory tract, gastric mucosa, blood vessels, and lymphatic organs and are known to be essential for allergic reactions such as allergic rhinitis, asthma and atopic dermatitis (Metcalfe DD, Kaliner M , Donlon MA, The mast cell, Crit. Rev. Immunol., 1981; 3: 23-74.10). Mast cells are activated through various receptors in the cell membrane (Metzger H, Alcaraz G, Hohman R, Kinet JP, Pribluda V, Quarto R. The receptor with high affinity for immunoglobulin E. Annu. Rev. Immunol. : 419-70). Activation of mast cells inducing degranulation of mast cells is induced by IgE receptor-mediated addition of compound 48/80, phorbol 12-myristate 13-acetate, a protein kinase C (PKC) activator, and calcium ionophore A23187 (Chand N, Pillar J, Diamantis W, Perhach JL, Sophia RD. Inhibition of calcium ionophore (A23187) stimulated histamine release from rat peritoneal mast cells by azelastine: implications for its mode of action. Eur J. Pharmacol., 1983; 96: 227-33; 11; Takei M, Umeyama A, Shoji N, Arihara S, Endo K. Mechanism of inhibition of IgE-dependent histamine release from rat mast cells by penasterol and penasterone J. Pharm Sci 1995; 84: 228-30. . The chemical mediators including histamine stored in the granules are liberated by the stimulation that induces the degranulation of mast cells. By this mediator, permeability enhancement and expansion action of peripheral blood vessels, expansion action on bronchial smooth muscle, The secretory action of the secretion of the allergic reaction is expressed. In addition to chemical mediators released from mast cells, inflammatory cytokines such as tumor necrosis factor (TNF) -α, interleukin (IL) -1β, IL-6 and IL-8 play a crucial role in the induction of these allergic inflammatory responses (Galli SJ, Gordon JR, Wershil BK, Cytokine production by mast cells and basophils, Curr. Opin. Immunol.1991, 3: 865-72, 13. Galli SJ, Tsai M, Piliponsky AM. of allergic inflammation. Nature 2008; 454: 445-54). Allergic reactions involve a variety of immune cells, including mast cells, which secrete inflammatory cytokines such as TNF-α and IL-6 (Arend WP, Dayer JM, Inhibition of the Production and Effect of Interleukin-1 and tumor necrosis factor alpha in rheumatoid arthritis Arthritis Rheum 1995; 38: 151-60). TNF-α is a multifunctional cytokine that is secreted when mast cells are activated and is involved in the immune and inflammatory responses and promotes the secretion of other inflammatory cytokines such as IL-1β, IL-6 and IL-8 (Barnes PJ , Adcock I. Modulation of proinflammatory cytokine release in the rheumatoid synovial membrane: a prospective, randomized, double-blind, placebo-controlled trial of anti-inflammatory actions of steroids: molecular mechanisms, Trends Pharmacol. Sci 1993; 14: 436-41; cell cultures. Comparison of monoclonal anti-TNF-a antibodies with the IL-1 receptor antagonist Eur. Cytokine Netw. 1995; IL-6 is also a cytokine that mediates a potent inflammatory response (Erchler WB, Keller ET, Age-associated increased IL-6 gene expression, late-life diseases, and frailty. 70), IL-8 is a chemoattractant that promotes the mobilization of neutrophils, lymphocytes and eosinophils, activates neutrophils to release lysosomal enzymes, induce active coral production, and is involved in inflammatory diseases such as arthritis and sepsis (Harada A, Mukaida N, Matsushima K. IL-8 as a novel target for interventional theraphy in acute inflammatory disease.

Mitogen-activated protein kinase (MAPK) and NF-κB are proinflammatory cytokines such as TNF-α, IL-1β, IL-6 and IL-8 (Galli SJ, Kalesnikoff J, Grimbaldeston MA, Piliponsky AM, Williams CM, Tsai M. Mast cells as a "tunable" effector), which is known to regulate the expression of cyne and to transmit stimuli from outside the cell and immunoregulatory cells: recent advances Annu Rev. Immunol 2005; 23: 749-86; Azzolina A, Bongiovannia, Lampiasi N. Substance p induces TNF-α and IL-6 production through NFκB peritoneal mast cells. Gallic acid inhibits histamine release and pro-aminotransferase activity in the rat liver, which is the major histocompatibility inhibitory factor (NKR) inflammatory cytokine production in mast cells. Toxicol. Sci. 2006; 91: 123-31).

PKC (protein kinase C) plays a major role in cell activity and function, and has been known in a number of studies (Spitaler M, Cantrell DA, Protein kinase C and beyond. Nature immunology 2004; 5: 785-90). In particular, it plays an important role in many aspects such as lymphocyte survival and activation differentiation in the immune response. For example, PKC is known to be involved in B-cell-mediated immune responses, and is involved in B cell activation through stimulation of B cell receptors. PKC is known to regulate the B cell immune response, (Tan SL, Parker PJ, Emerging and diverse roles of protein kinase C in immune cell signaling. Biochem J. 2003; 376: 545-52). Thus, the regulation of the secretion of these cytokines from mast cells can be a treatment for allergic inflammatory diseases.

(Castane crenata S. et Z.), which has been used as an antiseptic and a symbolic food since ancient times, is a fruit of a perennial tree, Castane crenata Lieb. Et. Zucc, Dried and used for pulp, most of which is starch and contains a small amount of protein and fat (Kim MJ, Lee, Hwang MS, Kim SC, Lee MH, Blooming, Fructification and nut characteristics of chestnut cultivated cultivated in korea. For Soc 2003; 92: 321-32). It is called "dry chestnut" or "黄 栗". Oriental medicine, dry chestnut (dried chestnut) is flat, flavor and taste a little salty. The moderate rate is good, and it improves the function of the masturbation and strengthens, and it treats the indigestion and the diarrhea. Hemorrhagic efficacy has also been used for hematemesis, nosebleed, and stool. It has the effect of loosening the blood ejaculation. If it is painful due to muscle or ligament damage, it swells and congested. In addition, the dry calorie, tingling, calming the muscular spasms, sedation, see the respiratory system and stop coughing, so it is useful to apply to chronic cough. If you look at the constitution, the dryness is good for a big body type, a character whose pace is constant and exceptionally sweaty. The hard shell of the night is called ゅgaku (chestnut shell). It is also used as a dye and relieves thirst due to diabetes. Leaf of chestnut is helpful when itching with lacquer disease or skin disease, and washing with boiled water when eczema happens. (Kim CM, Lee JS, An DK, Sim MK. The encyclopedia of China oriental herbal medicine. : Jungdamsa. 2006. 4372-4373).

Considering that about 30% of the night production is processed and exported, the waste amount is not negligible but the industrial utilization as a by-product is not developed. (Moon JS, A study of physicochemical properties of starch separated from chestnut inner shell waste, Dongshin University, 1999), inhibits the production of active oxygen, inhibits skin melanin formation, The purpose of this study was to investigate the efficacy of the extracts and solvent fractions for the inflammatory cytokine secretion of herbal cosmetics.

It has not been taught or described in the literature that the purified fraction isolated from the tuberous extract can be used as a composition for preventing and treating skin aging.

Accordingly, the inventor of the present invention found that the extract or the purified product of uri had excellent DPPH radical scavenging activity, increased activity of ABTS radical cation decolorization and superoxide dismutase (SOD) -like activity, The present invention has been accomplished by confirming the usefulness of the composition as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating skin aging.

In order to accomplish the above object, the present invention provides a dermatological pharmaceutical composition for the treatment and prevention of skin aging comprising an extract or a purified product as an active ingredient.

As used herein, "skin aging" includes wrinkles, spots, freckles, skin damage due to ultraviolet light, skin cancer, preferably skin damage due to ultraviolet light.

The extract of the present invention is characterized by containing 0.1 to 50% by weight based on the total weight of the dermatological pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In order to attain the above object, the present invention provides a cosmetic composition for improving and preventing skin aging, which comprises an extract or a purified product as an active ingredient.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

The tuberous extract as defined herein is prepared by mixing water, a lower alcohol having 1 to 4 carbon atoms, acetone, or a mixed solvent thereof, preferably water, ethanol, acetone or a mixed solvent thereof, more preferably a mixed solvent of water and ethanol, Still more preferably 30 to 90% water and ethanol mixed solvent or acetone, most preferably extractable in acetone,

More specifically, after drying and washing, the dried clay is washed and mixed with water, a lower alcohol having 1 to 4 carbon atoms, acetone, or a mixed solvent thereof, preferably water, ethanol, acetone or a mixed solvent thereof, more preferably water and ethanol More preferably 30 to 90% water and ethanol mixed solvent or acetone, most preferably acetone, and then mixed at a temperature of 0 ° C to 150 ° C, preferably 20 ° C to 100 ° C, for 30 Min to 72 hours, preferably 12 hours to 48 hours, by repeating ultrasonic extraction, hot water extraction, room temperature extraction or reflux extraction, preferably room temperature extraction, about 1 to 20 times, preferably 2 to 10 times, , Followed by filtration, concentration under reduced pressure, and drying.

The UFI purified product as defined herein includes a first step of fractionating the UFI extract into chloroform (chloroform), ethyl acetate, n-butanol and water fractions; The n-butanol was fractionated by fractionation (Fr) 1 to Fr. 20 using a Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100%). Fr. 1 (CIeB1), Fr. 2 (CIeB2), Fr. 3 (CIeB3), Fr. 4 (CIeB4), Fr. 5 (CIeB5), and 6 fractions; Fr. (HPLC conditions: column: YMC ODS-A 150 x 4.6 C18 Column (4 mu m), 150 x 4.6 mm, Waters Min; sample concentration: 1 mg / ml; column temperature: 40 占 폚); mobile phase: Solvent A: MeOH; solvent B: water in 1% HCOOH; detector: ultraviolet absorption spectrophotometer (UV 280 nm) In a peak between 2 and 4 minutes. Third fraction purified (CIeB3).

The extract or the purified product prepared by the above method showed excellent activity of DPPH radical scavenging activity, ABTS radical cation decolorization activity, and superoxide dismutase (SOD) -like activity, It has been confirmed that the composition is useful as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating skin aging.

The extract or the purified product is characterized by comprising 0.1 to 50% by weight based on the total weight of the skin pharmaceutical composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

Accordingly, the present invention provides an external dermatological pharmaceutical composition and a cosmetic composition for skin aging treatment and prevention, which comprises an extract or purified product obtained by the following production method and the production method as an active ingredient.

The extract of the present invention has long been used for herbal medicines and edible foods. The extract of the present invention has no problems such as toxicity and side effects, and has been proved to be a non-irritant sample in the skin patch test. Therefore, Can be used.

The dermatological pharmaceutical composition containing the extract or purified product of the present invention is prepared by a pharmaceutical composition in the form of cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma However, the present invention is not limited thereto.

The preferred dosage of the extract or tablet of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract or the purified product of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The extract or the purified product of the present invention can be used variously in cosmetics and cleansers having an anti-aging effect.

Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.

The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned compounds, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

As described above, the tuberous extract or the purified product of the present invention is excellent in DPPH radical scavenging activity, ABTS radical cation decolorization activity, and superoxide dismutase (SOD) -like activity increasing activity , The composition is useful as an external dermatological pharmaceutical composition or cosmetic composition for preventing and treating skin aging.

BRIEF DESCRIPTION OF THE DRAWINGS FIG.
FIG. 2 is a view showing a separation process of each fraction from a 70% ethanol extract; FIG.
Figure 3 shows the process of separating the purified product from the n-BuOH fraction of the CIe extract;
Figure 4 shows HPLC data of the CIe extract;
FIG. 5 is a graph showing the electron donating ability of a CI extract (BHA: Butylated hydroxyanisole (positive control), and the result is expressed by the mean (mean) SD of triplicate);
FIG. 6 is a graph showing the electron donating ability of fractions obtained from the CIeB extract (BHA: butylated hydroxyanisole (positive control), and the result is expressed by mean (mean). + -. SD);
Figure 7 shows the ABTS + cation radical scavenging activity of the CI extract (BHA: Butylated hydroxyanisole (positive control), the results being expressed as means (mean) ± SD of triplicate);
Figure 8 shows the ABTS + cation radical scavenging activity of the fractions from the CIe extract (BHA: Butylated hydroxyanisole (positive control), the results being expressed as mean (mean) SD of triplicate);
Figure 9 shows the ABTS + cation radical scavenging activity of the fractions from the CIeB extract (BHA: Butylated hydroxyanisole (positive control), the results being expressed as mean (mean) SD of triplicate);
Figure 10 is a graph showing the superoxide anion (O2-) radical scavenging activity of CI extract (BHA: Butylated hydroxyanisole (positive control), AA: ascorbic acid (positive control) (Means ± SD);
Figure 11 shows the superoxide anion (O2-) radical scavenging activity of fractions from the CIe extract (BHA: Butylated hydroxyanisole (positive control), AA: ascorbic acid Lt; / RTI > is expressed as mean (means) SD of triplicate);
Figure 12 shows the superoxide anion (O2-) radical scavenging activity of the fractions from the CIe extract (BHA: Butylated hydroxyanisole (positive control), AA: Ascorbic acid, Lt; / RTI > is expressed as mean (means) SD of triplicate);
Figure 13 shows the superoxide anion (O2-) radical scavenging activity of the fractions from the CIeB extract (BHA: Butylated hydroxyanisole (positive control), AA: Ascorbic acid, Lt; / RTI > is expressed as mean (means) SD of triplicate);
Figure 14 shows the hydrogen peroxide scavenging activity of the fractions from the CIe extract (BHA: Butylated hydroxyanisole (positive control), AA: ascorbic acid (positive control, ) ± SD);
Figure 15 is a graph showing the hydrogen peroxide scavenging activity of fractions from the CIe extract (BHA: Butylated hydroxyanisole (positive control), AA: ascorbic acid (positive control) ) ± SD);
Figure 16 shows the hydrogen peroxide scavenging activity of the fractions from the CIeB extract (BHA: Butylated hydroxyanisole (positive control), AA: ascorbic acid (positive control) ) ± SD).

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Reference Examples and Experimental Examples.

Example One. Julie Crude extract  Manufacturing (see Figure 1)

1-1. Preparation of acetone extract

The dried yeast was used by Daegu Technopark Bio Health Convergence Center. In the case of the acetone extract, 70% acetone was added in an amount of 10 times the weight of the sample and immersed at room temperature for 24 hours to separate the supernatant and the precipitate. 2 filter paper. The filtrate was concentrated using a vacuum rotary condenser and lyophilized to obtain an acetone extract (yield: 31.58%, hereinafter referred to as CIa).

1-2. Preparation of 70% ethanol extract

The 70% ethanol extract was also extracted by the same method, followed by filtration and concentration under reduced pressure to obtain a 70% ethanol extract (yield: 29.07%, hereinafter referred to as CIe).

1-3. Heat number  Preparation of extract

The hot-water extract was extracted with shaking at 85 ° C for 3 hours. 2 filter paper. The filtrate was concentrated using a vacuum rotary condenser and lyophilized to obtain a hot water extract (yield: 45.33%, hereinafter referred to as CIw).

Example  2. Julie  menstruum Fraction  And Purified water  Manufacturing (see Figures 2 and 3)

2-1. Julie  Preparation of ethanol extract fraction

From the acetone, ethanol, and hot water extracts extracted as described above, different solvents were added from the 70% ethanol extract having high inhibitory activity using the solvent polarity difference and fractionated stepwise.

UFI 70% ethanol extract and chlorform were added to the fraction funnel at a ratio of 1: 1 and fractionated with chlorofluoromethane. After repeated three times, the filtrate was concentrated under reduced pressure to obtain 0.05% yield of chlorform layer fraction (hereinafter referred to as CIeC) .

2, an ethyl acetate layer (hereinafter referred to as CIeE) 11.648%, an n-butanol layer (hereinafter referred to as CIeB) 42.668%, a water layer (hereinafter referred to as CIeW) 45.344% Each fraction was obtained sequentially.

2-2. Julie Fraction  detach

The fraction of n-BuOH as described above was fractionated in 500 ml fractions using Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100%). Fr. 1 (1.72%, hereinafter referred to as CIeB1), Fr. 2 (19.97%, hereinafter referred to as CIeB2), Fr. 3 (53.63%, hereinafter referred to as CIeB3), Fr. 4 (1.03%, hereinafter referred to as CIeB4), Fr. 5 (0.28%, hereinafter referred to as CIeB5) and Fr.6 (0.09%, hereinafter referred to as CIeB6). And the highest yield was obtained at the third CIeB3 (FIG. 3).

Experimental Example  1. Determination of total phenol and total flavonoid content

Phenolic compounds are one of the secondary metabolites widely distributed in plants and have various structures and molecular weights, and they have a property of binding with macromolecules such as proteins because they have phenolic hydroxy groups. As shown in Table 1, the yield of the powder obtained by lyophilization of the udder extract was 28.76% and the extract of the udder extract The total phenol content and the total flavonoid content were found to be 108.21 mg / g and 18.54 mg / g, respectively.

Extraction Yield and Total Phenol and Total Flavonoid Contents Measurment Water extraction Extraction yield (%) 28.76 Total polyphenol (mg / g) 108.21 + - 0.17 Total flavonoid (mg / g) 18.54 + - 0.01 All measurements were done in triplicate, and all values are mean ± standard deviation.

(HPLC conditions: column: YMC ODS-A 150 x 4.6 C18 Column (4 m), 150 x < RTI ID = 0.0 > 4.6 mm, Waters; Mobile phase: Solvent A: MeOH; Solvent B: Water in 1% HCOOH Detector: Ultraviolet absorption spectrophotometer (UV 280 nm) Flow rate: 1.0 ml / min Sample concentration: 1 mg / : 40 ° C)

As a result of the above HPLC analysis, CleB2 and CleB3 were analyzed by HPLC. Peak was detected at 2 to 4 minutes, and CleB2 content was 2 times higher than CleB3 by peak height. (See Fig. 4)

Experimental Example 2. Antioxidant Experiment

2-1. Measurement of DPPH radical scavenging ability

In order to test the antioxidative ability of DPPH radical scavenging ability of the samples obtained in the examples, the Blois method disclosed in the literature was applied as follows (Blois, MS 1958. Antioxidant determination by the use of a stable free radical. Nature 26 , 1199-1204.)

1,1 Diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method is mainly used for phenolic structure and aromatic amine compounds.

The DPPH alcohol solution has the strongest UV absorption at 517 nm and is a very stable free radical at room temperature. The specific absorption band of DPPH at 517 nm disappears as electrons or hydrogen radicals are taken from the electron donor to generate phenoxy radicals. The visible purple of DPPH turns yellow in proportion to the molarity of the fixed molecules.

These DPPHs are also stable in an alcohol solution, although there is a secondary or tertiary oxidation reaction in a nonpolar solvent such as dioxane or CCl4.

The electron donating ability was measured by Blois method as follows. To 100 μL of each sample solution, add 50 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl, and measure the absorbance at 517 nm for 30 minutes.

The activity of electron donating ability of 10 ug / ml 58% in acetone, ethanol, and hot-water extract of urine was shown in Fig. At a concentration of 1,000 ug / ml, the acetone extract showed 62% of the electron donating activity, but the activity was not significantly different from that at the low concentration.

1 ug / ml from CIeB1 1.72%, CIeB2 19.97%, CIeB3 53.63%, CIeB4 1.03%, CIeB5 0.28%, CIeB6 0.09% from the Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100% The activity of electron donating ability at a concentration of ~ 100 ug / ml is shown in Fig. The activity of electron donating ability increased as the concentration of uri fractions increased, indicating a significant difference in concentration activity. Especially, at the concentration of 5 ug / ml of CIeB3, it showed 70% or more electron donating activity.

2-2. ABTS Radical  Cation Decolorization  Measure

In order to test the antioxidant effect of the samples obtained in the examples by ABTS radical cation decolorization method, the method described in the literature was applied as follows (Roterta, R., P. Nicoletta, P. Anna , P. Ananth, Y. Min and RE Catherine 1999. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay.Radic.Biol.Me. 26 , 1231-1237.)

ABTS radical scavenging ability can measure both the hydrogen-donating antioxidant and the chain breaking antioxidant, which measure the relative antioxidant effects of the extracts.

Also, ABTS + free radicals generated by the reaction with potassium persulfate can be removed by antioxidants in the extract, so that relative comparison of extracts can be made by using standard materials. . ABTS ⁺ radical cation decolorization was measured by Pellegrin et al.

7 mM ABTS ⁺ 5 mL and 2.4 mM K ₂ S ₂ O ₈ were mixed with ethanol at a ratio of 1: 6 and the absorbance of the control was adjusted to 0.7 ± 0.002 at 734 nm using an ABTS ⁺ solution. 0.1 mL of the sample solution and 0.1 mL of ABTS ⁺ solution were mixed, reacted at room temperature for 7 minutes, and absorbance was measured at 734 nm.

The activity of ABTS radical scavenging activity of 10 ug / ml 80% of acetone, ethanol, and hot water extract of uri is shown in Fig. At 50 ug / ml concentration, acetone, ethanol and hot water extract showed 98% of ABTS radical scavenging activity.

The activity of ABTS radical scavenging activity at the concentration of 1 ug / ml ~ 100 ug / ml of ethyl acetate, n-butanol and water fractions is shown in FIG. The activity of ABTS radical scavenging activity increased as the concentration of uri fractions increased. Especially at the concentration of 10 ug / ml, the acetone extract showed 63% activity of ABTS radical scavenging activity.

1 ug / ml from CIeB1 1.72%, CIeB2 19.97%, CIeB3 53.63%, CIeB4 1.03%, CIeB5 0.28%, CIeB6 0.09% from the Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100% ≪ / RTI > to 100 ug / ml.

The activity of ABTS radical scavenging activity increased as the concentration of uri fractions increased. Especially, at the concentration of 5 ug / ml of CIeB3, ABTS radical scavenging ability was 70% or more.

2-3. Measurement of superoxide dismutase (SOD) -like activity

In order to test the antioxidant efficacy by the method of measuring the superoxide dismutase (SOD) -like activity of the samples obtained in the examples, Fidovich's method disclosed in the literature was applied as follows (Siddhuraju, P., Becker , K. (2007), The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata L.) seed extracts. J. of Food Chemistry. 101: 1019)

Superoxide anion radicals are considered to be the most important reactive oxygen species because a large proportion of other active oxygen is generated from superoxide anion radicals and is known to be the main radical causing cell damage, neuronal cell death, cancer and aging (Jayaprakasha , GK, RL Jaganmohan and KK Sakariah 2004. Antioxidant activities of flavin in different in vitro moder systems. Bioorganic & Medicinal Chem . 12 , 5141-5146.)

Superoxide anion radical scavenging ability was evaluated by the principle that when xanthine reacts with xanthine oxidase, color development is inhibited in the presence of antioxidants capable of removing O ² .

Superoxide anion radical scavenging activity was measured by Fidovich's method. Add 0.1 mL of each sample solution and 0.6 mL of 0.1 M potassium phosphate buffer (pH 7.5), add 1 mL of the substrate solution containing 0.4 mM xanthine and 0.24 mM nitro bluetetrazolium (NBT), add 1 mL of xanthineoxidase (0.049 U / mL) After reacting at 37 ° C for 20 minutes, 1 ml of 1 N HCl was added to terminate the reaction, and the amount of superoxide anion radical produced in the reaction solution was measured at 560 nm.

The activity of superoxide anion radical scavenging activity of 50% or more of 10 ug / ml of acetone, ethanol, and hot water extract of urine was shown in FIG. At 50 ug / ml concentration, acetone, ethanol and hot water extract showed superoxide anion radical scavenging activity of 80%.

The activity of the electron donating ability at a concentration of 1 ug / ml to 100 ug / ml of ethyl acetate, n-butanol and water fractions was shown in FIG. The activity of electron donating ability increased as the concentration of uri fractions increased, indicating a significant difference in concentration activity. Especially at the concentration of 10 ug / ml, acetone extract showed 63% electron donating activity.

The activity of superoxide anion radical scavenging activity at a concentration of 1 ug / ml to 100 ug / ml of ethyl acetate, n-butanol and water fractions is shown in FIG. The activity of superoxide anion radical scavenging activity increased as the concentration of uri fractions increased. Especially at the concentration of 100 ug / ml, ethyl acetate extract showed 89% superoxide anion radical scavenging activity.

1 ug / ml from CIeB1 1.72%, CIeB2 19.97%, CIeB3 53.63%, CIeB4 1.03%, CIeB5 0.28%, CIeB6 0.09% from the Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100% The activity of superoxide anion radical scavenging activity of ~ 100 ug / ml concentration is shown in Fig. The activity of superoxide anion radical scavenging activity increased as the concentration of uric acid isolate increased.

Especially, at the concentration of 5 ug / ml of CIeB1, CIeB2, and CIeB3 isolates, the activity of superoxide anion radical scavenging activity was over 60%.

2-4. H ₂ O ₂ Scatters Measure

H ₂ O ₂ of the samples obtained in the Examples (Jayaprakasha, GK, RL Jaganmohan and KK Sakariah, 2004) Antioxidant activities of flavinin in different in vitro moder systems. Bioorganic < RTI ID = 0.0 > Medicinal Chem . 12 , 5141-5146.)

H ₂ O ₂ is a cytotoxic substance, which causes severe damage to DNA in the organs of the body and causes various diseases and aging.

In addition, the toxicity of H ₂ O ₂ itself is low, but it can pass through the cell membrane and react with metal ions to produce hydroxyl radical which is highly toxic, which is a problem in the living body.

H ₂ O ₂ The scavenging ability was measured by Jayaprakasha et al. 0.5 mL of the sample was reacted with 0.5 mL of 40 mM H ₂ O ₂ at 37 ° C for 10 minutes, and the absorbance at 230 nm was measured.

Urea's acetone, ethanol, and hot water extract all contained 50 ug / ml 80% or more H ₂ O ₂ The scavenging activity is shown in Fig. Acetone in 1,000ug / ml concentration, ethanol, not the difference between the active and hot-water extract to open, high H ₂ O ₂ at low concentrations Indicating that it exhibits scavenging activity.

Ufi ethyl acetate, n-butanol, water fractions 1 ug / ml to 100 ug / ml H ₂ O ₂ The scavenging activity is shown in Fig. At the concentration of the uridine fraction of 5 g / ml, H ₂ O ₂ In particular, at a concentration of 50 ug / ml to 100 ug / ml, the uric acid ethyl acetate, n-butanol, and water fractions showed 80% or more H ₂ O ₂ Lt; / RTI > activity.

Ml to 1 μg / ml of CIeB1, CIeB2, CIeB3, CIeB3, CIeB4, CIeB4, CIeB5, CIeB5, and CIeB6 from Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100% 100 ug / ml H ₂ O ₂ The scavenging activity is shown in Fig. At a concentration 50g / ml of fraction yulpi CIeB2, CIeB3 extract more than 85% H ₂ O ₂ The activity of scavenging activity was rapidly increased. At a concentration of 100 ug / ml, H ₂ O ₂ When the scavenging activity was compared, there was a slight difference.

Experimental Example  3. Antibacterial experiment

In order to test the antimicrobial activity of the samples obtained in the examples, the Disc diffusion assay method disclosed in the literature was applied to the experiment as follows.

The antimicrobial activity of the urophyllite hydrothermal extract was measured by Disc diffusion assay method. The results are shown in Table 2. As the treatment concentration increased, the size of growth inhibition rings, which showed antibacterial activity, increased proportionally. Gram-negative bacteria, Pseudomonas aeruginosa Strain The antimicrobial activity of all the strains was 0.1, 1, 2 and 5 mg / disc, and the growth inhibition was 17 mm clear zone at the concentration of 5 mg / disc. Staphylococcus epidermis And Staphylococcus aureus The inhibition of growth was observed at a concentration of 1 mg / disc or more in the strain, and Samonella typhimurium , Escherichia Coli and Candida albicans showed growth inhibition at concentrations of 2 mg / disc or more and showed excellent antimicrobial activity at 5 mg / disc for all strains.

Antimicrobial activity Strains Water extraction (mg / disc) 0.1 One 2 5 Salmonella typhimurim - - ²⁾ 11 13 ¹⁾ Staphylococcus epidermis - 11 11 13 Staphylococcus aureus - 9 12 17 Escherichia coli - - 11 14 Candida albicans - - 11 13 Pseudomonas aeruginosa 10 21 27 27 ¹⁾ Inhibition zone in diameter (mm), ²⁾ No inhibition

Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.

Formulation Example  1. Cream composition

The oil phase and water phase are heated to 75 ° C and cooled to room temperature.

Formulation Example  2. Massage Cream  Composition

The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.

Formulation Example  3. lotion composition

The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.

Formulation Example  4. Skin lotion composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  5. Essence composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  6. Pack composition

The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.

Formulation Example  7. Cleansing Foam  Composition

The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (7)

A skin external pharmaceutical composition for the treatment and prevention of skin aging, which comprises an extract or a purified water as an active ingredient. [Claim 2] The composition for external application for skin according to claim 1, wherein the tubular extract is an extract extractable in water, a lower alcohol having 1 to 4 carbon atoms, acetone, or a mixed solvent thereof. [2] The method of claim 1, wherein the purified water comprises a first step of separating the tubercle extract into chloroform (chloroform), ethyl acetate, n-butanol and water fractions; The n-butanol was fractionated by fractionation (Fr) 1 to Fr. 20 using a Diaion HP-20 column chromatography (H ₂ O: EtOH = 0 → 100%). Fr. 1 (CIeB1), Fr. 2 (CIeB2), Fr. 3 (CIeB3), Fr. 4 (CIeB4), Fr. 5 (CIeB5), and 6 fractions; Fr. (HPLC conditions: column: YMC ODS-A 150 x 4.6 C18 Column (4 mu m), 150 x 4.6 mm, Waters Min; sample concentration: 1 mg / ml; column temperature: 40 占 폚); mobile phase: Solvent A: MeOH; solvent B: water in 1% HCOOH; detector: ultraviolet absorption spectrophotometer (UV 280 nm) In a peak between 2 and 4 minutes. Third fraction purified (CIeB3). [Claim 2] The skin pharmaceutical composition according to claim 1, wherein the skin aging is skin wrinkles, spots, freckles, skin damage due to ultraviolet rays, or skin cancer. The dermatological pharmaceutical composition according to claim 1, wherein the pharmaceutical composition comprises a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta or a cataplasma. A cosmetic composition for improving and preventing skin aging, which comprises an extract or a purified water as an active ingredient. The cosmetic composition according to claim 6, wherein the cosmetic composition is a formulation of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack.

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