KR20140043544A - Ant-stress composition using an extract of xylosma congesta - Google Patents

Abstract

Disclosed is an anti-stress composition using an Xylosma congesta extract. The Xylosma congesta extract used in the present invention recovers a stress phenomenon such as an increase in weight of an adrenal gland, a decrease in weight of a spleen, an increase in the number of red blood cells and white blood cells, and an increase in GPT, GOT, and LDH levels when administered to experimental animals that have received stress. [Reference numerals] (AA) Normal group; (BB) Fatigue-induced group

Description

Anti-stress Composition Using an Extract of Xylosma congesta

The present invention relates to a process for the preparation of It relates to an antistress composition using the congesta ) extract.

Stress is defined as a decrease in physical and mental ability, and is classified into physical stress (fatigue), which means mental stress, which means imbalance of body rhythm due to mental overload, and decreased ability to perform exercise Development of Functional Evaluation System for Fatigue Recovery of Health Functional Foods, Research Report).

Mental stress causes various symptoms such as tension headache, migraine headache, hypertension, dyspepsia, fatigue, pain, menopausal disorder, hair loss, skin roughness, etc., and when chronic persistent, various diseases including peptic ulcer, hypertension, diabetes, . ≪ / RTI > Especially, mental stress causes anxiety and emotional distress.

The physical response to this mental stress begins by stimulating the function of the sympathetic pituitary gland (HPA) and inducing hormone secretion. The adrenal glands stimulated by adrenocorticotropic hormone are increased in weight, and the spleen is significantly reduced in weight due to the decline of immune function. In addition, the adrenal glands secrete other steroid hormones and secrete blood cholesterol, blood sugar, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aspartate aminotransferase (AST or) GOT), and alanine transaminase (ALT or GPT). These stress responses ultimately reduce the homeostasis of the body and weaken the body's immune system, thus causing various diseases (Friedman et al., Psychosom Med. 1963, 25: 364-376; Snel J et al. Physiol. 1972, 65: Suppl: 328A; Groth W et al., Zentralbl Veterinarmed A. 1975, 22 (1): 57-75; Gold et al., Psychoneuroendocrinology 1985, 10 (4): 401-19; S et al., 1988, 20 (1): 36-42).

Physical stress causes fatigue by accumulating lactic acid in muscles. The accumulation of lactic acid involves muscle-mediated LDH (lactate dehydrogenase), which catalyzes the production of lactic acid from pyruvic acid. Physical stress increases LDH and causes accumulation of lactic acid in muscles (Fitts et al., Am J Physiol. 1976, 231 (2): 430-3; Savitski et al., Ukr Biokhim Zh. ): 45-9).

Ginsenosides Rg3 and Rg2 (Korean Registered Patent No. 1120996), Astragalus Extract (Korean Patent Publication No. 10-2009-0073462), Water Lily Extract (Korean Registered Patent No. 876440), etc. It is proposed to have activity.

The present invention discloses the anti-stress activity of the citron extract.

It is an object of the present invention to provide a composition for anti-stress.

Other and further objects of the present invention will be described below.

As the present inventors confirmed in the following Examples and Experimental Examples, when the stress on the experimental animals, the adrenal weight increases, the spleen weight decreases, the leukocyte count increases, GPT, GOT and LDH levels increase It was confirmed that, when the oleander extract is administered, these phenomena were recovered in a concentration-dependent manner.

The present invention is provided based on the experimental results, the anti-stress composition of the present invention is characterized in that it contains an acid oil extract as an active ingredient.

In the present specification, "acid oil extract" refers to an acid oil stem, leaf, fruit, flower, root, a mixture thereof, and the like, which is an extract, lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, butanol, ethylene, acetone, Hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or mixed solvents thereof are used Refers to a crude extract obtained by fractionating the crude extract and one or more of the solvents listed above. The extraction method can be applied by any method such as cold-rolling, refluxing, heating, ultrasonic irradiation or the like in consideration of the degree of extraction and the degree of preservation of the active ingredient. In the case of the fractionated extract, the crude extract is suspended in a specific solvent and mixed and fixed with a solvent having a different polarity. The crude extract is adsorbed onto a column filled with silica gel and the like, followed by a hydrophobic solvent, a hydrophilic solvent, or a mixture thereof. It is meant to include a fraction obtained by using a solvent as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably it refers to an extract obtained by using water, ethanol or a mixed solvent thereof as the extraction solvent, more preferably an extract obtained by using a mixed solvent of water and ethanol as the extraction solvent.

In addition, in the present specification, "active ingredient" means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

In addition, in the present specification, "antistress" refers to the recovery of mental fatigue and physical fatigue, in particular, the meaning of relaxation of tension, relaxation of anxiety, improvement of concentration and / or improvement of exercise ability.

The composition for anti-stress of the present invention may include any effective amount (effective amount) according to the use, formulation, compounding purpose, etc. as long as the active ingredient can exhibit the anti-stress activity, the typical effective amount is based on the total weight of the composition It will be determined in the range of 0.001% to 99.990% by weight. Herein, "effective amount" refers to the amount of active ingredient capable of inducing an anti-stress effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The composition for antistress of the present invention is extracts / powders of the Chinese herb extracts / powders, buckthorn branches, leaves, roots, or mixtures thereof, in addition to the oil extract, which is an active ingredient, for the reinforcement and increase of antistress activity. , Extracts / powders of rhododendron branches, leaves, roots or mixtures thereof, locust starch extracts / powders, quarantine starch extracts / powders, white bellflower outpost extracts / powders, camphor branches, leaves, roots or mixtures thereof, extracts / powders, islands Extracts / powders of organulpi branches, leaves, roots or mixtures thereof, extracts / powders of deoderm stems, leaves, roots or mixtures thereof, and the like. Here, the meaning of the extract is as described above in connection with the citron extract. Such extracts may be obtained by mixing the extract objects listed above.

In addition, the anti-stress composition of the present invention ginseng ( Panax known to have a fatigue recovery activity, immunity enhancement activity, etc., in addition to the oil extract of the active ingredient ginseng ) extract / powder (Bahrke et al., Sports Med 2000, 29: 113-33), ginseng fibrinase (cellulase, pectinase, hemicellulase, etc.) or polysaccharide degrading enzymes (endo-xylase) , Beta-glucanase, glucoamylase, polygalactronase, arabinase, xylase, β-glucosidase, α-arabinosidase, α-ramnosidase, α-amylase, etc.) treatment Water, Aspergillus sp., Rhizopus sp., Yeasts (especially Saccaromyces) cerevisiae ), Bacillus sp. in particular B. subtilis , Monascus sp. in particular M. purpureus , Lactobacillus sp ., Bifidobacterium sp ., Pediococcus sp ., Leuconostoc sp., Bifidobacterium sp . Etc, especially Lactobacillus plantarum , Lactobacillus bulgaricus, Lactobacillus casei , Lactobacillus acidophilus , Bifidobacterium bifidum ) or fermented products by the mixed bacteria thereof, and extracts / powders of seeds, leaves, fruits, stems or mixtures thereof of ginseng.

The ginseng as used herein means ginseng, red ginseng or white ginseng obtained by steaming and / or drying the ginseng, and the extract is as described above with reference to the citron extract.

The anti-stress composition of the present invention can be identified as a food composition in a specific embodiment.

When the antistress composition of the present invention is identified as a food composition, the form of the food can be selected from the group consisting of tea, juice, carbonated beverage, beverage such as ionic beverage, milk processed, processed oil such as root, gum, rice cake, , Foods such as cotton, powders, tablets, capsules, and the like.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

The anti-stress composition of the present invention can be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may be formulated into a wide variety of dosage forms, including pharmaceutically acceptable carriers, excipients and the like, in addition to the active ingredient, and may be formulated into a wide variety of preparations such as creams, lotions, ointments (semi-solid external medicine) (TTS) (e.g., patches, bandages, etc.), and the like.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, and the like. One or more may be selected and used according to the formulation of the pharmaceutical composition of the present invention. For suitable pharmaceutically acceptable carriers and formulations, see Remington's. Pharmaceutical Sciences (19th ed., 1995). The pharmaceutical compositions of the present invention may further comprise emulsifiers (such as TWEENS), wetting agents (such as sodium lauryl sulfate), colorants, flavors, stabilizers, preservatives, water, saline, phosphate buffer solutions, and the like.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

As described above, according to the present invention can provide a composition for anti-stress using an acid extract.

The anti-stress composition of the present invention can be produced into food or medicine.

1 is a result showing that the oil extract extracts the concentration-dependent recovery of the contraction of the spleen by stress.
2 is a result showing that the oil extract extracts the concentration-dependent recovery of the hypertrophy of the adrenal gland by stress.
3 and 4 show that the citron extract recovers concentration-dependently the increase in the number of white blood cells and the number of red blood cells due to stress, respectively.
5 to 7 show that the oil extract extracts the concentration-dependent recovery of GPT, GOT and LDH levels, respectively.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Production of citron extract

10 g of dried and pulverized citrus leaf powder was added to 70% ethanol and extracted with stirring at room temperature for 48 hours. After filtration, the obtained filtrate and the extract residue separated by filtration were added to 70% ethanol and stirred at room temperature for 24 hours. After sonification, the filtrate obtained by filtration was mixed, and the mixture was concentrated under reduced pressure and lyophilized to obtain a solid citron extract.

< Experimental Example > Of citron extract Antistress  Active experiment

<1> Experimental Method

<1-1> experimental animals

Six-week-old male Sprague-Dawley male rats (COREAC, Korea) were purchased, and only one healthy animal was allowed to acclimate for one week before the start of the experiment to allow the animals to adapt to the changed environment, temperature, humidity, and food. The experiment was started.

The breeding environment of the experimental animal room was adjusted to a temperature of 22 ± 2 ℃, relative humidity of 45 ± 10%, 10-15 cycles / hr of ventilation, 12 hours of illumination, illumination of 150-250 Lux, and noise of 60dB or less. Polycarbonate was kept in three rabbits per week and the box was changed twice a week to keep the ammonia concentration below 20ppm. The feed was autonomously fed Harlen's sterilized solid feed, and the negative water was autoclaved sterilized water for free intake. Ten rats were used in each experimental group.

<1-2> Evaluation of fatigue recovery efficacy

The experimental group was classified into the normal group (stress non-induced group) and the stress group in group 4, and the group of stress-induced group 3 was treated with low concentration (100 mg / kg), medium dose (200 mg / kg) and high concentration ( 400mg / kg) was divided into citron extract and drinking water was administered orally once a day for 14 days (Table 1.)

Classification of the entire experimental group Group Treatment No. of Rat I Normal
Drinking Water-> 1.2 mg / mari PO 10 Ⅱ Stress-induced group (control)
Drinking Water-> 1.2 mg / mari PO 10 Ⅲ Citron low concentration group
-> 1.2 mg / mari PO 10 IV Mid-birth concentration group
-> 1.2 mg / mari PO 10 V Dairy high concentration group
-> 1.2 mg / mari PO 10

<1-3> Measurement of antistress labeling factors

The samples were administered for 14 days, subjected to restraint stress, and collected for blood biochemical components. Plasma ALT (GPT), AST (GOT), and LDH levels were measured using an automated blood biochemical analyzer (Hitachi, model name Hitachi 7020). In addition, after 14 days of sample administration and restraint stress, organ weights such as spleen and adrenal gland were measured.

The anti-fatigue effect was carried out for 18 hours by immobilization stress test on the 14th day of the experiment (Yakhak Hoeji, 1995, 39 (5): 548-553; Korean J. Orient. Int. Med. 2001, 22 ( 4): 683-689). Restraint stress was performed in a restraint cage 20cm in height and 15cm in diameter (Dae-Biolink, model name DH-SC03) for 18 hours on day 14 (p.m 3 o'clock the next day a.m 9 o'clock).

&Lt; 2 & Experiment result

<2-1> organ weight

The organ weights of the spleen and adrenal glands of each experimental group were measured and shown in [FIG. 1] and [FIG. 2]. 1 and 2, in the stress-induced group, spleen contraction and adrenal hypertrophy were observed in the stress-induced group compared to the normal group (non-stress-induced group). It can be seen that the contraction and hypertrophy of the adrenal glands are suppressed.

<2-2> white blood cell count

The number of white blood cells (granulocyte and monocyte) of each experimental group was measured and shown in [FIG. 3] and [FIG. 4]. 3 and 4, an increase in leukocyte count was observed in the stress-induced group compared to the normal group (non-stress-induced group), and it can be seen that concentration-dependent recovery was achieved in the oxidized extract administration group.

<2-3> concentration of blood marker

GPT, GOT, and LDH levels of each experimental group were measured and shown in FIGS. 5 to 7. 5 to 7, the increase in the levels of these factors was observed in the stress-induced group compared to the normal group (non-stress-induced group), it can be confirmed that the concentration-dependent recovery in the citron extract administration group. have.

Claims (4)

Antistress composition comprising an acid oil extract as an active ingredient.
The method of claim 1,
The acid citron extract is an antistress composition, characterized in that obtained by using an oil citron leaf as an extraction target and using water, ethanol or a mixed solvent thereof as an extraction solvent.
3. The method according to claim 1 or 2,
Wherein said composition is a food composition.
3. The method according to claim 1 or 2,
Wherein the composition is a pharmaceutical composition.

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