KR20130107967A - Composition containing natural extracts - Google Patents

Abstract

PURPOSE: A composition containing a natural extract is provided to show anti-inflammation and antioxidation effects using Lonicera japonica Thunberg, Lithospermum erythrorhizon, Castanea crenata, and Sophora flavescens Solander ex Aiton extracts, thereby suppressing oxidation and removing toxicity. CONSTITUTION: An anti-inflammatory or antioxidative composition contains Lonicera japonica Thunberg, Lithospermum erythrorhizon, Castanea crenata, and Sophora flavescens Solander ex Aiton extracts as active ingredients, which are mixed in a weight ratio of 1-10:1-10:1-10:1-10. The composition contains 0.001-30 wt% of the extracts.

Description

Composition containing natural extracts {COMPOSITION CONTAINING NATURAL EXTRACTS}

The present invention relates to a composition comprising a plant extract of natural origin, and more particularly to a composition having anti-inflammatory, antioxidant and / or edema relief.

As human skin ages, the secretion of various hormones that control metabolism decreases, and the function and function of cells of immune cells is reduced, thereby reducing the biosynthesis of immune proteins and bioconstituent proteins necessary for the living body. As aging progresses, symptoms of changing or decreasing the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which constitute the skin, appear. As a result, the skin is subjected to oxidative stress by free radicals and active noxious oxygen, thereby destroying and thinning the skin matrix.

In addition, in modern society, the risk of disease or condition due to inflammation is increasing due to irregular life, increased contamination, and expression of novel strains.

Accordingly, the inventors have completed the present invention by confirming that the composition containing the extracts of honeysuckle, soybean, yulpi and ginseng as active ingredients not only has anti-inflammatory and anti-oxidant properties, but also alleviates or suppresses swelling when applied to the skin. .

Republic of Korea Patent Publication No. 10-2001-0054290

An object of the present invention is to provide a composition having anti-inflammatory, antioxidant and / or edema relief.

In order to achieve the above object, the present invention provides a composition comprising an extract of Phosphorus, Licorice, Yulpi and Gosam as an active ingredient.

The composition of the present invention includes an extract of phosphorus, licorice, yulpi and ginseng as an active ingredient, as well as having an anti-inflammatory effect, and has an antioxidant ability, thereby inhibiting or delaying oxidation, thereby having an effect on detoxification. In addition, the composition of the present invention is useful because it has the effect of inhibiting or alleviating edema. In particular, the mixed extract of the present invention has a superior anti-inflammatory and antioxidant capacity by mixing phosphorus, licorice, yulpi and ginseng can alleviate edema.

Honeysuckle (忍冬) herein, the honeysuckle and honeysuckle (Lonicera japonica Thunberg ) or other stems and branches of related plants. Indong flowers bloom in May-June, pale reddish white, but later turn yellow, and hang on the axillas and smell. Corollas are lip-shaped, 3-4 cm long. Corollas are divided into 5 at the end, flipped back, and have a dense hair on the outside. Beneath the flowers are leaf-shaped poinsacks. Fruits are round as berry and ripen black from October to November. Even in winter, the leaves do not fall off depending on where they are called.

In the present specification, the herbaceous (紫草) is a perennial herbaceous plant of the lichen ( Lithospermum erythrorhizon ) roots. Licorice helps blood circulation and restores refreshment and taste.

In the present specification, yulpi (栗 皮) refers to the inner skin of the fruit of the beech beech tree ( Csatanea crenata ). to be. Applying yulpi to honey will shrink the skin and smooth out the wrinkles.

Ginseng (苦 參) in the present specification is the root of the cane ( Sophora flavescens ), a perennial herb of legumes. Ginseng is used for dysentery, lobulation, genital pruritus, itching of the skin caused by moist and low heat, and when urinary pain due to bladder fever.

The present invention relates to an anti-inflammatory composition comprising an extract of honey, soybean, yulpi and ginseng as an active ingredient in one aspect. Since the composition of the present disclosure has anti-inflammatory activity, it is effective in treating and / or preventing diseases caused by invasion of various bacteria by immune response against various bacteria. Specifically, the composition of the present disclosure has anti-inflammatory activity by delaying or inhibiting the production of prostaglandins, but is not limited thereto.

In another aspect, the present invention relates to an antioxidant composition comprising phosphorus, licorice, yulpi and ginseng extract as active ingredients. The compositions herein have the effect of delaying or stopping the mechanism of known oxidation reactions in the human body. Specifically, the composition of the present invention exhibits an effect of inhibiting oxidation by inducing and / or promoting expression of one or more genes selected from the group consisting of GSTs, NQO1, and PRDX1, but is not limited thereto.

In still another aspect, the present invention relates to a edema relief composition comprising an extract of honey, soybean, yulpi and ginseng as an active ingredient. The composition of the present disclosure has the effect of alleviating the edema generated in the entire area of the human body as well as the face and / or inhibit, delay the production of edema. Therefore, the composition of the present invention is useful for relieving edema after sleep and lower limb edema due to prolonged activity.

Extraction of each natural product in the present specification, for example, washed and dried fine granulated copper, soybean, yulpi and red ginseng extract in water or an organic solvent, extracted and deposited, and then filter and centrifuged the residue and the filtrate. The separation may be obtained by separating the filtrate under reduced pressure, and the present invention is not limited thereto, and may be obtained by using an organic solvent extraction method, distillation under reduced pressure, and the like, which are generally used in the art. The organic solvent usable for obtaining the extract of the present specification may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, water or 30 to 70%. Preference is given to using concentrations of ethanol. After obtaining the extract using a solvent as described above, the extract can be obtained by cooling, heating and filtering at room temperature by a conventional method known in the art, or the solvent can be further evaporated, spray dried or lyophilized.

In the composition of one aspect of the present invention, the extract may be obtained by mixing a honey extract, a licorice extract, a yulpi extract, and a ginseng extract. That is, the composition of the present invention may include a mixed extract obtained by extracting the extract for each of the phosphorus, licorice, yulpi, ginseng, and then mixed.

In the composition of one aspect of the present invention, the extract may be obtained from a mixed extract of phosphorus, licorice, yulpi and red ginseng. That is, the composition of the present invention may include a mixed extract obtained by extracting the phosphorus, licorice, yulpi and ginseng at once.

In one aspect of the present invention, the extract may be contained in 0.001 to 30% by weight relative to the total weight of the composition. When the extract of the present invention is contained in less than 0.001% by weight relative to the total weight of the composition, the improvement of efficacy such as prevention of skin inflammation and alleviation of skin swelling is insignificant, and when it is contained in an amount exceeding 30% by weight, the increase in efficacy is insignificant. Formulation stability may deteriorate and skin irritation may occur. From the above point of view, the extract of the present invention may be contained in 0.005 to 25% by weight, 0.01 to 20% by weight, 0.05 to 15% by weight or 0.1 to 10% by weight relative to the total weight of the composition.

In the composition of the aspect of the present invention, the extract may be a weight ratio of phosphorus: sorghum: yulpi: gooseberry 1 ~ 10: 1 ~ 10: 1 ~ 10: 1 ~ 10. When the weight ratio of the extract is out of the above range, the increase in efficacy is insignificant compared to the increase in content according to the present invention and formulation stability may be deteriorated. From the above point of view, the extract of the present invention has a weight ratio of phosphorus: coniferous: yulpi: ginseng 1 to 9: 1 to 9: 1 to 9: 1 to 9, 1 to 7: 1 to 7: 1 to 7: 1 7, 1-5: 1-5: 1-5: 1-5, or 1-3: 1-3: 1-3.

In a composition which is one aspect of the present invention, the composition includes an external preparation composition for skin.

The composition of the present invention may be a topical skin composition, and more specifically, may be used as a topical skin composition for anti-inflammatory, anti-aging and / or edema.

When the composition for external application for skin according to the present invention is formulated in the form of a cosmetic composition, it may be formulated into cosmetic formulations such as softening lotion, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack And the formulations thereof are not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In one aspect of the invention, the composition may be a cosmetic composition.

When the composition according to the present invention is formulated in the form of a cosmetic, it may be formulated in the form of a softening agent, a convergent lotion, a nutritional lotion, an eye cream, a nutritional cream, a massage cream, a cleansing cream, a cleansing foam, a cleansing water, a powder, And the formulations thereof are not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In one aspect of the present invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

In addition, the dosage of the active ingredient will vary depending on the age, sex and weight of the subject to be treated and the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical dosages are from 0.001 mg / kg / day to 2000 mg / kg / day, more specifically from 0.5 mg / kg / day to 1500 mg / kg / day.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[ Comparative Example 1 ] Indwelling  Preparation of extract

5 L of 70% ethanol aqueous solution was added to 1 kg of phosphorus, and the mixture was extracted under reflux three times, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 89 g of a phosphorus extract.

[ Comparative Example 2 Preparation of Licorice Extract

5 L of a 70% ethanol aqueous solution was added to 1 kg of porcelain, and refluxed three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 75 g of a perilla extract.

[ Comparative Example 3 ] Julie  Preparation of extract

5 l of a 70% ethanol aqueous solution was added to 1 kg of yulpi, extracted under reflux three times, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 92 g of the extract of Yulpi.

[ Comparative Example 4 ] Gosam  Preparation of extract

5 kg of 70% ethanol aqueous solution was added to 1 kg of red ginseng, and refluxed three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 81 g of red ginseng extract.

[ Example 1 ] Indwelling , Julie , Gosam  Preparation of Mixed (1: 1: 1: 1) Extract

5 kg of 70% ethanol aqueous solution was added to 1 kg (mixing ratio = 1: 1: 1: 1) of commercially available phosphorus, soybean, yulpi, and ginseng extracts obtained in Comparative Example 1-4, followed by extraction under reflux three times. It was deposited for 1 day at ℃. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 81 g of a mixture of phosphorus, soybean, yulpi and red ginseng (1: 1: 1: 1).

[ Example 2 ] Indwelling , Julie , Gosam  Preparation of Mixed (9: 9: 1: 1) Extracts

5 kg of 70% ethanol aqueous solution was added to 1 kg (mixing ratio = 9: 9: 1: 1) of the commercial phosphorus, soybean, yulpi, and ginseng extracts obtained in Comparative Example 1-4, and refluxed three times. It was deposited for 1 day at ℃. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the filtrate was concentrated under reduced pressure to obtain 89 g of a mixture of honey, soybean, yulpi and red ginseng (9: 9: 1: 1).

[ Test Example  One] Indwelling , Julie , Gosam  Anti-inflammatory test of mixed extract

The anti-inflammatory effects of the mixtures of honeysuckle, soybean, yulpi, and ginseng were evaluated by measuring the inhibitory effect of prostaglandin production using electrocultured macrophages. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 mM, thereby irreversibly inhibiting cyclooxygenase (COX) activity remaining in the cells. Then, 100 μl of the cell suspension was added to each well of a 96 well plate and then incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. The attached macrophages were then washed three times with PBS and used for anti-inflammatory tests. In addition, RPMI medium containing 1% (w / v) of LPS (lipopolysaccharide) was added to 5 × 10 ⁴ cells / ml of electrocultured macrophages, followed by incubation for 12 hours to induce the production of prostaglandins. Phosphorus, Licorice, Yulpi, Ginseng Mixture Extract of Example 2, and each phosphorus, Licorice, Yulpi, and Ginseng Extract according to Comparative Examples 1-4 were diluted in purified water with 0.5% each and treated with 100 μl of free prostaglandin, respectively. Quantification was performed using enzyme immunoassay (ELISA). At this time, RPMI medium containing 1% (w / v) of LPS (lipopolysaccharide) was added to 5 × 10 ⁴ cells / ml of the cultured macrophages, followed by incubation for 12 hours to induce the production of prostaglandins, and aspirin (Bayer) Was diluted in purified water at 0.1% and treated with 100 ((Comparative Example 5) to quantify the free prostaglandin using the enzyme immunoassay (ELISA) was used as a positive control. Prostaglandin production inhibitory activity of the extract was set to 100% the difference between the prostaglandin produced in the LPS-treated and untreated groups, respectively, the percentage of prostaglandin reduced by treating each sample with LPS Table 1 Shown in

Inhibitory Effect of Prostaglandins Blank 100% Aspirin-treated group (Comparative Example 5) 25% Honeysuckle, Licorice, Yulpi, Ginseng Mixture Extract (Example 1) 46% Honeysuckle, Licorice, Yulpi, Ginseng Mixture Extract (Example 2) 47% Honeysuckle extract (Comparative Example 1) 19% Licorice Extract (Comparative Example 2) 22% Yulpi Extract (Comparative Example 3) 15% Ginseng Extract (Comparative Example 4) 24%

Example 1 or Example 2 containing a mixture of phosphorus, licorice, yulpi, and ginseng extract according to the present invention is 200-300% or more improved anti-inflammatory than Comparative Example 1-4 containing each of the extracts of phosphorus, licorice, yulpi, and ginseng In particular, it was found to show an effect of about 200% more than the conventional anti-inflammatory aspirin (Comparative Example 5). In particular, Comparative Example 1-4 was observed to show a lower level of anti-inflammatory effect than aspirin, while the mixed extract of Example 1 or Example 2 was found to show a superior anti-inflammatory effect than aspirin, the present invention It can be seen that it has an excellent effect by the mixture of licorice, yulpi and ginseng.

[ Test Example  2] antioxidant induction effect

HaCaT cells, which are human keratinocytes, were distributed and used by Korean Cell Line Bank (Seoul, Korea). HaCaT cells were injected into DMEM medium containing 10% (v / v) FBS, 100 U / ml penicillin and 100 μg / ml of streptomycin and cultured in an animal cell incubator with 37 ° C., 5% CO ₂ feed conditions. HaCaT cells prepared at a concentration of 1.5 × 10 ⁶ per well were adapted for 3 hours with medium without FBS. Thereafter, the extracts of Example 1 and Example 2 were respectively pretreated at 10 ppm concentration for 2 hours and further incubated for 8 hours by adding LPS at 1 µg / ml concentration. At this time, each comparative example was pretreated at 10 ppm concentration for 2 hours, and further cultured for 8 hours by adding LPS at 1 µg / ml concentration to be used as a control. In addition, the untreated group of wounded cells were also used as a control. Total RNA was extracted using TRIzol ™ (GIBCO BRL, MD, USA) and stored at −80 ° C.

1 μg total RNA was placed in 25 μl reverse transcription reaction buffer containing 50 mM Tris-HCl (Pris-HCl, pH 8.3), 75 mM KCl, 3 mM MgCl 2, 0.1 M DTT, 10 mM dNTP and 40 units / μL RNase inhibitor, 0.5 μg / Μl oligo (dT) 16 primers and 200 units of SuperScript II (GiboBRL) reverse transcriptase were added and reacted at 42 ° C for 1 hour. AmpliTaq) DNA polymerase [0.04U, Perkin Elmer, Shelton, CT], 50 mM Tris, pH 8.3, 0.25 mg / ml bovine serum albumin, 3 mM MgCl 2, 0.25 mM dNTPs, SYBR Green I 50 ml of PCR reaction buffer containing 1 / 50,000 dilutions of [Molecular Probes, Eugene, OR] were mixed, and 10 μM of primer was added thereto to denature at 94 ° C. for 30 seconds and at 53 ° C. for 30 seconds. 30 cycles of annealing and expansion at 72 ° C. for 1 minute were performed.

Relative mRNA levels were analyzed by measuring SYBR Green I fluorescence changes using ICycler software. Each primer was prepared based on published SCI-level papers by analyzing a substance having detoxification activity, and each base sequence is shown in Table 1 below. Quantitative expression levels of genes were corrected using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as an internal standard.

The primer sequences used in PCR Gene / NCBI Gene ID Primer Sequence number Amplification Product Size (bp) PCR reaction temperature (℃) denaturalization Annealing expansion GAPDH / 2597 5'-ATC CCA TCA CCA TCT TCC AG-3 ' One 579 94 58 72 5'-CCT GCT TCA CCA CCT TCT TG -3 ' 2 NQO1 / 1728 5'-TGA AGG ACC CTG CGA ACT TTC-3 ' 11 185 95 61 70 5'-GAA CAC TCG CTC AAA CCA GC-3 ' 12 GSTP1 / 2950 5'-AGG ACC TCC GCT GCA AAT AC-3 ' 13 105 95 60 70 5'-GGG TCT CAA AAG GCT TCA GTT G-3 ' 14 PRDX1 / 5052 5'-CGG AGA TCA TTG CTT TCA GTG A-3 ' 15 113 95 60 70 5'-AGG TGT ATT GAC CCA TGC TAG AT-3 ' 16

Expression of translational genes such as GSTs, NQO1 and PRDX1 has been reported to induce cell protective action from oxidative damage (Xie C, Lovell MA, Xiong S, Kindy MS, Guo J, Xie J, Amaranth V, Montine TJ, Markesbery WR.Expression of glutathione-S-transferase isozyme in the SY5Y neuroblastoma cell line increases resistance to oxidative stress.Free Radic Biol Med. 2001 Jul; 31 (1): 73-81; Liu Y, Kern JT, Walker JR, Johnson JA, Schultz PG, Luesch H. A genomic screen for activators of the antioxidant response element.Proc Natl Acad Sci US A. 2007 Mar; 104 (12): 5205-10; Carcinogenesis. 2000 May; 21 (5): 1013- 6.Induction of murine intestinal and hepatic peroxiredoxin MSP23 by dietary butylated hydroxyanisole.Ishii T, Itoh K, Akasaka J, Yanagawa T, Takahashi S, Yoshida H, Bannai S, Yamamoto M). Table 3 shows the effect of inducing the expression of GSTs, NQO1 and PRDX1 genes by extracts of honeysuckle, soybean, yulpi and red ginseng extract according to the comparative example and complex extracts mixed in a weight ratio of 1: 1: 1: 1 according to the examples. The measured result (relative value Fold).

Experimental Example NQO1 GSTP1 PRDX1 No treatment One One One Wound induction 0.8 0.5 0.6 Honeysuckle, Licorice, Yulpi, Ginseng Mixed Extract (Example 1) 2.7 3.5 2.8 Honeysuckle, Licorice, Yulpi, Ginseng Mixed Extract (Example 2) 2.9 3.4 2.7 Honeysuckle extract (Comparative Example 1) 1.5 1.8 1.9 Licorice Extract (Comparative Example 2) 1.3 1.4 1.5 Yulpi Extract (Comparative Example 3) 1.1 1.7 1.7 Ginseng Extract (Comparative Example 4) 1.3 2.0 1.3

As can be seen in Table 3, the mixed extract of Example 1 or Example 2 according to the present invention was observed to express a gene having an antioxidant capacity, compared to the comparative example containing only each extract. In particular, the mixed extract of Example 1 or Example 2 is confirmed to improve the expression of the GSTs, NQO1 and PRDX1 gene by 200-300% than the comparative example, the present invention is a mixture of honey, soybean, yulpi, ginseng It can be seen that it has an excellent effect.

[ Test Example  3]

[ Test Example  3-1] to improve swelling Cosmetics  Preparation of the composition

Comparative Examples 6 and 3-4, which are creamy emulsions, were prepared in the amounts shown in Table 4 below.

First, the oil-based parts consisting of raw materials 1 to 7 were placed in a separate container, heated and dissolved at 70 ° C., and dispersed using a homogenizer to prepare a lipophilic mixture. Water No. 9 was added to purified water by dispersing evenly in water part (raw materials 8 ~ 9) to dissolve while heating and dissolving to 75 ℃ and adding lipophilic mixture and emulsifying with oil-in-water type at 70 ℃ using homogenizer for 4 ~ 5 minutes. Caused. Phosphorus, licorice, yulpi, ginseng mixed extract, and other thickeners and an appropriate amount of flavor were added and mixed using a homogenizer for 3 minutes. The bubbles in the emulsion were removed using a degasser, the emulsion was filled in an airtight container and cooled to room temperature using a cooling unit.

NO Compounding ingredient Comparative Example 6 Example 3 Example 4 One Cetearyl alcohol 0.4 0.4 0.4 2 Glyceryl stearate One One One 3 C14-22 Alcohol / C12-20 Alkyl Glucoside 1.2 1.2 1.2 4 Stearic acid One One One 5 Glyceryl stearate / phage-100 stearate 0.5 0.5 0.5 6 Squalane 7 7 7 7 Dimethicone One One One 8 Purified water to 100 to 100 to 100 9 Glycerin (concentrated glycerin) 7 7 7 10 Honeysuckle, Sorrel, Yulpi, Ginseng Mixed Extract (Example 1) 0 5 0 11 Honeysuckle, Sorrel, Yulpi, Ginseng Mixed Extract (Example 2) 0 0 5 11 Other thickeners, neutralizers Suitable amount Suitable amount Suitable amount 12 Preservative, incense Suitable amount Suitable amount Suitable amount

[ Test Example  3-2] Measure the effect of improving face swelling

The facial swelling improvement effect was confirmed using Comparative Example 6 and Example 3-4. 40 women in their 30s were divided into two sets of 20 people, one group using Comparative Example 6 and Example 3 and the other group using Comparative Example 6 and Example 4, and each face was divided into two halves each morning and evening for two weeks. The cream of the comparative example and the Example was corrected, respectively. Using a PRIMOS Body device that can measure the volume of the skin, the volume of the skin was measured before and after two weeks of use, and the skin volume difference was calculated from the initial state to confirm the improvement of skin swelling. The results are shown in Table 5 below.

Face volume reduction (improve swelling) effect (ml) Comparative Example 6 1.5 Example 3 8.4 Example 4 8.2

It was confirmed that the mixed extract of Example 3 or Example 4 according to the present invention has a edema alleviation effect of at least five times compared to Comparative Example 6. Therefore, the mixed extract of the present invention has the effect of alleviating the edema generated in the body or inhibit the production of edema.

The soybean extract-containing composition according to the present invention is applicable to various formulations as follows, but is not limited thereto.

Preparation Example 1 Tablet

150 mg of ginseng, soybean, yul, and ginseng mixed extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were added and 40 mg of 30% ethanol was added to form granules. It was dried and compressed into tablets using a tablet press.

[Formulation Example 2]

150 mg, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed, 100 mg of 30% ethanol was added to form granules, and then dried at 60 ° C. to form granules. The next gun was filled. The final weight of the contents was 1 g.

[Formulation Example 1] Lotion Compounding ingredient weight% Honeysuckle, Sorghum, Yulpi, Ginseng Mixed Extract 3.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Water soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 1,3-butylene glycol 3.00 Purified water to 100

[Formulation Example 2] Pack Compounding ingredient weight% Honeysuckle, Sorghum, Yulpi, Ginseng Mixed Extract 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Lauroylhydroxyproline 1.00 Water soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water to 100

[Formulation Example 3] Compounding ingredient weight% Honeysuckle, Sorghum, Yulpi, Ginseng Mixed Extract 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Concentrated glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water to 100

[Formulation Example 4] ointment Compounding ingredient Content (% by weight) Honeysuckle, Sorghum, Yulpi, Ginseng Mixed Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

Anti-inflammatory composition comprising an extract of phosphorus, licorice, yulpi and ginseng as an active ingredient. Antioxidant composition comprising phosphorus, licorice, yulpi and ginseng extract as active ingredients. Edema relief composition comprising honeysuckle, licorice, yulpi and ginseng extract as active ingredients. The composition according to any one of claims 1 to 3, wherein the extract is obtained by mixing a honeycomb extract, a licorice extract, a yulpi extract, and a red ginseng extract. The composition of any one of claims 1 to 3, wherein the extract is obtained from a mixed extract of honeysuckle, soybean, yulpi and ginseng. The composition of any one of claims 1 to 3, wherein the extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The composition of any one of claims 1 to 3, wherein the extract has a weight ratio of Phosphorus: Licorice: Yulpi: Ginseng 1 to 10: 1 to 10: 1 to 10: 1 to 10. The composition according to any one of claims 1 to 3, wherein the composition is a topical skin composition. The composition according to any one of claims 1 to 3, wherein the composition is a cosmetic composition. The composition of claim 1, wherein the composition is a pharmaceutical composition.