KR20130093919A

KR20130093919A - Method for increasing protopanaxadiols of panax ginseng

Info

Publication number: KR20130093919A
Application number: KR1020120015165A
Authority: KR
Inventors: 안덕종
Original assignee: 경상북도(농업기술원)
Priority date: 2012-02-15
Filing date: 2012-02-15
Publication date: 2013-08-23

Abstract

The present invention relates to a method of increasing the content of protopananaxadiol (PD) -based saponin of ginseng by irradiating the LED of 440 ~ 470 nm to the washed ginseng.
According to the present invention, protopanaxadiol-based saponins are greatly increased only by irradiation of simple LEDs, rather than complicated and time-consuming methods such as steaming. , Obesity inhibition and anti-tumor.

Description

Method for Increasing the Content of Propananax Diol (PD) Saponin in Ginseng {Method for Increasing Protopanaxadiols of Panax ginseng}

The present invention relates to a method of increasing the content of protopananaxadiol (PD) -based saponin of ginseng by irradiating the LED of 440 ~ 470 nm to the washed ginseng.

Ginseng (Panax ginseng C.A.meyer) is usually grown for 4-6 years, and the natural ginseng mined natural ginseng contains about 75% water. Ginseng is collected from the main fabric and washed and then shipped in the state of fresh ginseng or dried and distributed for long-term storage.

Ginseng contains 60-70% of carbohydrates such as starch, and contains a large amount of ginseng saponin (ginsenoside), a major pharmacological component of ginseng. The saponin component is widely distributed in plants, and the ginseng contains triterpenoid damala

As a dammarane saponin, it is present only in plants of the genus Panax. In addition, it contains components such as polyacetylene, aromatic compounds, and acidic peptides.

Ginsenoside, which has the largest physiological activity among ginseng, has various beneficial effects such as anti-inflammatory, antioxidant and anti-cancer effects. Ginsenosides are peculiar saponins that exist only in the genus Ginseng, Protopanaxadiols (PDs; Rb1, Rb2, Rc, Rd, Rg3, Rh2) and Protopanaxtriols (PTs; Rg1, Rg2, Re, Rf, Rh1) is classified as a series, depending on the type of ginseng ginsenoside constituents 2 ~ 20% difference appears. In addition, the composition ratio of ginsenosides of the parts of ginseng also appears.

Pharmacological action on the central nervous system is known to show PD-type saponin sedation and PT-based saponin to moderate excitability (Nam 1996).

Among PD-based saponins, ginsenoside Rb1 promotes the secretion of corticosteroids and corticosteroids, and is known to have analgesic, anti-anxiety, anticonvulsion, memory improvement, promote neuronal survival, inhibit lipid peroxidation and anti-inflammatory effects. have. Ginsenoside Rb2 stimulates sugar and fat metabolism, lowers anti-diabetic, high cholesterol, inhibits smooth muscle cell proliferation, stimulates the secretion of adrenal cortical stimulating hormone and corticosterone, inhibits neovascularization, immunomodulation, hepatocellular proliferation, and platelet aggregation. It is known to do.

In addition, ginsenoside Rc promotes the synthesis of DNA, RNA, proteins and lipids of bone marrow cells, analgesic action, corticosteroid secretion and renal glomerular hypertrophy. Ginsenoside Rd is also known to stimulate the secretion of corticosteroids and corticosteroids and to inhibit renal glomerular hypertrophy. On the other hand, ginsenoside Rg3 is known to have anticancer and immune function regulating action.

As such, saponins belonging to ProtoPanaxadiol (PD) show a very important physiological activity, and there is a known method of increasing PD lineage by steaming ginseng. Steaming is complicated and takes a long time. .

Therefore, there is a need in the art for a method of providing ginseng with high content of protoparnasadiol (PD) -based saponins.

On the other hand, among the studies on the light environment affecting the growth and morphology of plants, in recent years, the calorific value is very low and has only a wavelength range that affects the growth and morphology of plants. There is a growing interest in light irradiating systems (Brown et al., 1995; Miyashita et al., 1995, Park et al., 2007).

LED has long lifespan, easy to drive pulse, small amount of sensible heat generation and long emission of long wave radiation, so it is suitable as artificial light source for plant production.

Moreover, LED can be used as a point light source, a line light source or a surface light source according to an arrangement method, and since it has a characteristic of a monochromatic light source as a small size, it has been used as an artificial light source for controlling the formation of light forms of plants (Brown et al., 1995; Okamoto; In addition, there are mercury-free, environmentally friendly, light weight, excellent power saving, and simple driving circuits (Hwang et al., 1996). , 2004).

The present invention is to increase the saponin content of the PD system, as well as increase the saponin content by irradiating LED of a specific wavelength to ginseng.

The present invention is to provide a method of increasing the content of protopananaxadiol (PD) -based saponin of ginseng by irradiating the LED of 440 ~ 470 nm to the washed ginseng.

According to the present invention, it is possible to increase the content of the protoparnaxadiol (PD) -based saponin of ginseng, which is an effective pharmacological component, by simply irradiating a simple LED rather than a complicated and time-consuming method such as steaming.

1 is a structural formula of ProtoPanacodiol,
2 is a structural formula of ProtoPanaanatriol.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example 1 LED Irradiation

Fresh ginseng was washed as a raw material and placed in a dark room, a room temperature of 4 ° C., and a chamber irradiated with LEDs of 380, 440, 470, and 660 nm for 7 days.

Example 2 Saponin Content Measurement

After 7 days, each ginseng was ground with a mixer and passed through a 30 mesh sieve to obtain a powder.

100 g of 80% ethanol was added to 2 g of powder, and the components of ginseng were extracted, followed by 3

Repeated reflux cooling for 2 times, and then filtered and concentrated under reduced pressure to prepare a ginseng extract composition.

The ginseng extract composition was extracted using 20 ml of water-saturated n-butanol, dissolved in 5 ml methanol, filtered through a 0.45 μm membrane filter (MFS-13, advantec MFS, Inc. Tokyo. Japan), and analyzed by HPLC. .

Table 1 Changes of ginsenosides (PT)

As can be seen in Table 1, PT-type saponins were untreated (4 ° C) upon LED irradiation. It can be seen that there is no significant increase compared to.

Table 2 Changes of ginsenosides (PD)

As can be seen in Table 2, saponins of the PD line shows a large increase at 440 and 470 nm compared to no treatment (4 ° C).

Table 3 Changes of total saponins

As can be seen in Table 3, the total saponin content was also significantly increased compared to the no treatment (DARK).

[Table 4] Changes of PD / PT

As can be seen in Table 4, after examining the LEDs, saponins in the system increased, and the result was increased.

In conclusion, when irradiated with LED 440 and 470nm light for 7 days, the total saponin as well as the PD / PT ratio were increased compared to no treatment.

Claims

Method of increasing the content of the proto-paranasadiol (PD) -based saponin of ginseng by irradiating the LED of 440 ~ 470 nm to the washed ginseng.

The method of claim 1,
The irradiation is a method for increasing the content of protopananaxadiol (PD) -based saponin of ginseng, characterized in that made for 7 days.