KR20130050096A - Device for inducing photodynamic reaction and method for inducing photodynamic reaction using the same - Google Patents

Abstract

PURPOSE: An apparatus for inducing photodynamic reaction and a method for inducing photodynamic reaction using the same are provided to prevent deformation of a subject through selective expression of optical energy and to ensure portability and mobility. CONSTITUTION: An apparatus for inducing photodynamic reaction comprises: a light source unit(20) which is mounted to irradiate a subject with optical energy; a power supply unit(30) which is mounted to supply power to the light source unit; a light control unit(40) which is mounted between the light source unit and the power supply unit to control intensity of the optical energy; and an optical energy wavelength cutting filter(50) which is mounted at the lower portion of the light source unit. The light source unit is an LED or an OLED which emits the optical energy.

Description

Device for inducing photodynamic reaction and method for inducing photodynamic reaction using the same

The present invention relates to a photodynamic reaction induction device and a photodynamic reaction induction method using the same.

A photodynamic reaction refers to a reaction in which photosensitive molecules absorb light to generate reactive oxygen species (ROS). The photodynamic reaction may be used to decompose and synthesize chemicals, or to grow, suppress, kill, or necrosize living organisms. Recently, photodynamic therapy (PDT) has been performed using photodynamic reactions. The photodynamic therapy (PDT) is a method of treating diseased cells or tissues by administering photosensitive molecules to lesions and irradiating light to generate reactive oxygen species (ROS). In other words, when a photosensitive molecule absorbs light of an appropriate wavelength, it transitions to an excited singlet electron state, some of which become fluorescent at the bottom state, and the rest of the triplet through a transition process between the systems. And then transfer to oxygen molecules through energy or electron transfer processes to generate active oxygen, including singlet electron state oxygen (singlet oxygen, ¹ O ₂ ). In addition, the light-sensitive photosensitive molecules transfer energy to surrounding substrate molecules to generate reactive free radicals or radical ions, and they react with oxygen molecules to react with superoxide anion radicals (ex. O 2 ⁻ ) and hydroxyl radicals ( ex. OH ⁻ ). The ¹ O ₂ , O ₂ ^- and OH ^- are reactive oxygen species, have strong activity, and can be treated by destroying diseased cells and tissues.

Conventionally, a tungsten lamp, a xenon lamp, a halogen lamp, or the like is used as a light source for inducing the photodynamic reaction as described above. However, since such light sources generate light energy in a wide wavelength region including ultraviolet light, there is a problem that selective excitation of photosensitive molecules for inducing a photodynamic reaction is difficult. There was a problem that deformation of the subject occurs as it is irradiated to the subject such as a substance or an organism.

Recently, in order to overcome the above problems, a method using a filter or a laser that selectively transmits only light energy in a wavelength region required has been developed. However, such an optical energy supply system has a problem that the price is high and the volume is relatively large, it is not easy to move and carry, and requires operation by a skilled technician. In addition, in the case of an optical energy supply system such as a laser, a high heat generation of the light source itself may affect the object, and attentive environmental conditions are required, and thus there is a problem in that the application range is limited.

Accordingly, the deformation of the object can be prevented through the expression of light energy in the selective wavelength region, the portability and mobility are easy, the uniform light energy of a large area can be provided, the reproducibility is excellent, and the surrounding environment There is a need for the development of a photodynamic reaction induction device having little influence on the condition.

An object of the present invention is to provide an apparatus for inducing photodynamic reaction and a method for inducing photodynamic reaction using the same.

The present invention provides a means for solving the above problems, the light source unit provided to irradiate light energy to the object; A power supply unit installed to supply power to the light source unit; A light control unit installed between the light source unit and the power supply unit to adjust the intensity of light energy radiated from the light source unit; And an optical energy wavelength cut filter installed under the light source unit to selectively block a portion of a wavelength region of the light energy emitted from the light source unit.

As another means for solving the above problems, the present invention provides a method for inducing photodynamic reaction comprising irradiating light energy to an object containing photosensitive molecules using the photodynamic reaction induction device according to the present invention. do.

As another means for solving the above problems, the present invention provides a method for inducing photodynamic reaction comprising irradiating light energy to a mixture of photosensitive molecules and an object using the photodynamic reaction induction device according to the present invention. do.

The device for inducing photodynamic reaction and the method for inducing photodynamic response using the same can prevent deformation of an object through selective expression of light energy, provide portability and mobility, and provide a large area of uniform light energy. It can do it, it is excellent in reproducibility, it has little influence on surrounding environment, and it can control light energy quantitatively.

1 is a view showing the configuration of a photodynamic reaction induction device according to an embodiment of the present invention.
FIG. 2 is a diagram illustrating a schematic diagram in which a photosensitive molecule and an object are filled in a lattice shape in a 48 well plate used in Examples 1 and 2 of the present invention.

The present invention is a light source unit installed to irradiate light energy to the object; A power supply unit installed to supply power to the light source unit; A light control unit installed between the light source unit and the power supply unit to adjust the intensity of light energy radiated from the light source unit; And an optical energy wavelength cut filter disposed below the light source unit to selectively block a portion of a wavelength region of the light energy radiated from the light source unit.

Hereinafter, an apparatus for inducing photodynamic reaction of the present invention will be described in detail with reference to the accompanying drawings.

1 is a view showing the configuration of a photodynamic reaction induction device according to an embodiment of the present invention.

As shown in FIG. 1, the photodynamic reaction induction device 10 according to an embodiment of the present invention is largely divided into a light source unit 20, a power supply unit 30, a light control unit 40, and an optical energy wavelength cut. It may include a filter 50, and may further include a reaction vitreous unit 60, the pedestal 70 and the support 80. Specifically, the power supply unit 30 and the light source unit 20 are electrically connected through the light control unit 40, the light energy wavelength cut filter 50 is installed under the light source unit 20, the light A reaction vitreous unit 60 is installed below the energy wavelength cut filter 50, and a pedestal 70 supporting the reaction vitreous unit 60 is provided below the reaction vitreous unit 60. 20, the light energy wavelength cutting filter 50 and the pedestal 70 may be connected through the support 80.

The photodynamic reaction induction device of the present invention can induce a uniform photodynamic response by irradiating a large area of uniform light energy to an object, and has excellent repeat reproducibility, and expresses light energy in a selective wavelength region. It is possible to induce a photodynamic response of the subject while preventing deformation of the subject.

In the present invention, the light source unit may supply the light energy required to induce a photodynamic reaction in the object. In order for the photodynamic reaction to be induced, photosensitive molecules and light energy in a suitable wavelength range capable of generating reactive oxygen species from the photosensitive molecules are required. The light source unit serves to supply light energy capable of generating active oxygen species from the photosensitive molecules, and the type of the light source unit is not particularly limited, and may be generally used in the art, but preferably light It may be an LED or OLED emitting energy. When the LED or the OLED is used as the light source unit, a large area of uniform light energy may be irradiated to the object, and the reproducibility of the photodynamic reaction may be improved.

In the present invention, when using the LED or OLED as the light source unit, the wavelength of the light energy emitted from the LED and the OLED is not particularly limited, and appropriately selected according to the absorption wavelength region of the photosensitive molecules used to induce photodynamic reactions. It may be, but preferably 400 nm to 900 nm. If the wavelength of the light energy emitted from the LED and the OLED is less than 400 nm, it may cause necrosis of an organism such as a cell by the light energy, and if it exceeds 900 nm, the water present in the object by the long wavelength is stretched Heating may cause deformation of the subject.

In the present invention, the power supply may supply power to the light source unit so that the light source unit can irradiate the light energy to the object.

The type of the power supply is not particularly limited, and may use all of the power supplies generally used in this field, but preferably, a portable battery or an AC power supply may be used. When the portable battery is used as the power supply unit, portability and mobility of the photodynamic reaction induction device according to the present invention can be improved. The type of the portable battery is not particularly limited, and for example, a rechargeable battery such as a dry battery or a secondary battery can be used.

In the present invention, the light control unit may be installed between the light source unit and the power supply unit to adjust the intensity of the light energy irradiated from the light source unit. The light control unit enables quantitative light energy irradiation, and can easily induce photodynamic reaction selectively according to the object or condition through quantitative light energy irradiation. The type of the light control unit is not particularly limited, and any type can be used as long as it is generally used in this field.

In the present invention, the optical energy wavelength cut filter is provided under the light source unit to selectively block a portion of the wavelength region of the light energy irradiated from the light source unit, thereby enabling selective excitation of the photosensitive molecules, ultraviolet light It is possible to prevent deformation of an object caused by light energy in an unnecessary wavelength region such as a region.

The wavelength region of the light energy selectively blocked by the optical energy wavelength cut filter is not particularly limited and may be appropriately selected according to the absorption wavelength region of the photosensitive molecules used to induce the photodynamic reaction. Specifically, for example, when using a pheophorbide-A having a maximum absorption wavelength range of 580 nm to 680 nm as a photosensitive molecule, an optical energy wavelength cut filter capable of selectively blocking a wavelength of less than 580 nm. It is preferable to use, and when using hematoporphyrin having a maximum absorption wavelength range of 500 nm to 600 nm, it is preferable to use an optical energy wavelength cutting filter capable of selectively blocking wavelengths of less than 480 nm. May be, but is not limited thereto.

The photodynamic reaction induction device of the present invention may further include a reaction vitreous portion. The reaction vitreous part is a part for supporting the photosensitive molecules and the object, and expresses the photoactivity of the photosensitive molecules through the optical energy selectively transmitted through the optical energy wavelength cutting filter, thereby realizing a photodynamic reaction. Can provide.

The reaction vitreous part may carry a photosensitive molecule and an object. The kind of the photosensitive molecule is not particularly limited, and any kind can be used as long as it is generally used in the art, but preferably the maximum absorption wavelength range is 400 nm to 900 nm, more preferably 500 nm to 700 nm. Phosphorus photosensitive molecules can be used, and examples thereof include porphyrin-based compounds or phthalocyanine-based compounds, and more preferably 5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum ( 5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum, PtCP), 5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (5,15 -bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (t-PtCP), tetraphenyl porphyrin (H2TPP), hematoporphyrin (HP), protopo rphyrin (PP) , Indocyanin green (ICG), meso-tetrakis (p-sulfonatophenyl) porphyrin (meso-tetrakis) and one or more selected from the group consisting of (p-sulfonatophenyl) porphyrin (TSPP) and pheophorbide A.

As used herein, the term "subject" refers to a substance involved in the photodynamic reaction generated by the photosensitive molecule. In the present invention, a chemical substance or an organism may be mentioned as the subject.

The kind of the chemical is not particularly limited, but may be a phenolic compound including bisphenol A, which is preferably defined as an environmental hormone, and the kind of the organism is not particularly limited, but is preferably a cancer cell, a smooth muscle cell, a blood vessel. May be at least one selected from the group consisting of endothelial cells, Gram-positive bacteria, Gram-negative bacteria and viruses.

The photodynamic reaction induction device of the present invention may further comprise a support. The support may serve to support the light source unit, the light energy wavelength cut filter, and the reaction vitreous unit.

The present invention also relates to a photodynamic reaction induction method comprising the step of irradiating light energy to a subject containing a photosensitive molecule using the photodynamic reaction induction apparatus according to the present invention described above.

In the photodynamic reaction induction method of the present invention, the subject containing the photosensitive molecule may be an organism to which the photosensitive molecule is administered. When irradiating light energy to the organism to which the photosensitive molecule is administered using the photodynamic reaction induction device according to the present invention, the photosensitive molecule in the organism can absorb the light energy to express the photoactivity, and by the photoactivity Cells in an organism can grow, inhibit, kill or necrosis.

The organism to which the photosensitive molecule is administered is not particularly limited, and any organism can be used as long as the life phenomenon is maintained, but preferably may be an animal other than a human.

The kind of photosensitive molecules administered to the organism is not particularly limited, and any type of photosensitive molecules may be used as long as they are commonly used in the art. Preferably, the maximum absorption wavelength range is 400 nm to 900 nm, more preferably 500. Photosensitive molecules ranging from nm to 700 nm can be used, and examples thereof include porphyrin-based compounds or phthalocyanine-based compounds, more preferably 5,10,15-triphenyl-20- (4-carboxyphenyl). -Porphyrin platinum (5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum, PtCP), 5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (t-PtCP), tetraphenyl porphyrin (H2TPP), hematopophyrin (HP), protopophyrin (protoporin) rphyrin (PP), indocyanin green (ICG), meso-tetrakis (p-sulfonatope ), And the at least one selected from the group consisting of porphyrin (meso-tetrakis (p-sulfonatophenyl) porphyrin, TSPP) and peoh formate carbide A (A pheophorbide).

The above-described photodynamic reaction induction method of the present invention can improve the reproducibility of the photodynamic reaction by not only irradiating uniform light energy to the organism to which the photosensitive molecules are administered, but also by irradiating light energy in a selective wavelength region. .

The present invention also relates to a photodynamic reaction induction method comprising the step of irradiating light energy to a mixture of photosensitive molecules and an object using the photodynamic reaction induction device according to the present invention described above.

In the photodynamic reaction induction method of the present invention, the subject may be a chemical or an organism.

The kind of the chemical is not particularly limited, but may be a phenolic compound including bisphenol A, which is preferably defined as an environmental hormone, and the kind of the organism is not particularly limited, but is preferably a cancer cell, a smooth muscle cell, a blood vessel. May be at least one selected from the group consisting of endothelial cells, Gram-positive bacteria, Gram-negative bacteria and viruses.

The kind of the photosensitive molecule is not particularly limited, and any kind can be used as long as it is generally used in the art, but preferably the maximum absorption wavelength range is 400 nm to 900 nm, more preferably 500 nm to 700 nm. Phosphorus photosensitive molecules can be used, and examples thereof include porphyrin-based compounds or phthalocyanine-based compounds, and more preferably 5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum ( 5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum, PtCP), 5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (5,15 -bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum (t-PtCP), tetraphenyl porphyrin (H2TPP), hematoporphyrin (HP), protopo rphyrin (PP) , Indocyanin green (ICG), meso-tetrakis (p-sulfonatophenyl) porphyrin (meso-tetrakis) and one or more selected from the group consisting of (p-sulfonatophenyl) porphyrin (TSPP) and pheophorbide A.

The above-described photodynamic reaction induction method of the present invention can improve the reproducibility of the photodynamic reaction by not only irradiating uniform light energy to the mixture of the photosensitive molecules and the object, but also irradiating light energy in a selective wavelength region. .

[ Example ]

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited by the following examples.

Example  One

In order to find out whether a uniform photodynamic reaction can be induced through a large-area uniform light energy irradiation, as shown in FIG. 1, a chemical reaction using a photodynamic reaction induction device according to an embodiment of the present invention An experiment was performed to decompose trichlorophenyl (TCP, trichlorophenyl).

Specifically, as a light source, a red LED emitting light energy in a wavelength region of 560 nm to 780 nm was irradiated for 2 hours at an intensity of 20 mW, and an optical energy wavelength cut filter capable of selectively blocking light energy wavelengths of less than 580 nm. The light energy wavelength of 580 nm or more among the light energy wavelengths of the red LED was irradiated to the reaction vitreous part. A 48 well plate was used as the reaction vitreous part, and as shown in FIG. 2, pheophorbide A (1.34), which is a photosensitive molecule in each well in the form of a lattice in the 48 well plate, was formed. 10 × ^{3 −3} M) and the subject TCP (6.18 × 10 ⁻⁵ M) were added, and photodynamic reactions were induced by using the light energy of 580 nm or more to be irradiated, thereby performing decomposition of TCP.

The TCP degradation rate in the 48 well plate of FIG. 2 attached through the TCP degradation experiment was summarized in Table 1 below.

A B C D E F G H One 29.0% × 29.0% × 32.3% × 31.9% × 2 × 29.3% × 30.7% × 30.1% × 30.1% 3 29.7% × 30.8% × 30.9% × 30.9% × 4 × 30.8% × 31.1% × 30.3% × 31.0% 5 29.0% × 30.6% × 30.2% × 30.6% × 6 × 31.7% × 29.6% × 29.2% × 30.8% ×: empty well

As shown in Table 1, TCP decomposition experiments were performed using the photodynamic reaction induction device according to the present invention. As a result, TCP was decomposed according to the photodynamic reaction in each well, and measured in each well. The overall mean value of TCP resolution was 30.4% and the maximum deviation was within ± 1.9%. Therefore, when the photodynamic reaction is induced using the photodynamic reaction induction device according to the present invention, it can be seen that the photodynamic reaction can proceed uniformly in all parts of the reaction element part by irradiating a large area of uniform light energy. Can be.

Example  2

In order to find out whether repeated reproduction of the same photodynamic reaction induction is possible, the chemical substance trichlorophenyl (TCP, trichlorophenyl) using the photodynamic reaction induction device according to one embodiment of the present invention, as shown in FIG. The experiment to decompose was performed.

Specifically, TCP decomposition experiments were performed in the same manner as in Example 1, but were repeated 10 times in the same manner as in Example 1 to determine the reproducibility of photodynamic reaction induction.

The maximum deviation of TCP degradation rate in the attached 48 well plate of FIG. 2 measured through the TCP degradation experiment is summarized in Table 2 below.

A B C D E F G H One ± 1.1 × ± 1.1 × ± 1.2 × ± 1.3 × 2 × ± 1.3 × ± 1.2 × ± 1.2 × ± 1.1 3 ± 1.2 × ± 1.3 × ± 1.3 × ± 1.2 × 4 × ± 1.4 × ± 1.2 × ± 1.4 × ± 1.2 5 ± 1.5 × ± 1.2 × ± 1.3 × ± 1.3 × 6 × ± 1.3 × ± 1.1 × ± 1.3 × ± 1.4 ×: empty well

As shown in Table 2, the TCP decomposition repeated experiments were performed using the photodynamic reaction induction device according to the present invention. As a result, TCP was decomposed according to the photodynamic reaction in each well, and in each well. The maximum deviation of the measured TCP resolution was within ± 1.4%. Therefore, when the photodynamic reaction is induced using the photodynamic reaction induction device according to the present invention, it can be seen that the photodynamic reaction can proceed reproducibly in all parts of the reaction vitreous part.

Example  3

In order to find out whether the photodynamic reaction can be induced in living organisms using the photodynamic reaction induction device according to the present invention, as shown in FIG. 1, the photodynamic reaction induction device according to one embodiment of the present invention is used. The experiment was performed to kill the organism E. coli (gram negative).

Specifically, as a light source, a red LED emitting light energy in the wavelength range of 560 nm to 780 nm was irradiated for 20 minutes at an intensity of 10 mW, and an optical energy wavelength cut filter capable of selectively blocking light energy wavelengths of less than 580 nm. The light energy wavelength of 580 nm or more among the light energy wavelengths of the red LED was irradiated to the reaction vitreous part. Pheophorbide A (3.37 × 10 ^-5 M), which is a photosensitive molecule, and E. coli (E. coli, gram negative), which is a subject, are placed in the cell culture device, which is the reaction vitreous part, and using the light energy of 580 nm or more wavelength to be irradiated. The photodynamic reaction was induced and the killing of E. coli was carried out.

Example  4 and 5

E. coli was killed in the same manner as in Example 3, except that the light energy irradiation time using the red LED was changed to 30 minutes (Example 4) and 60 minutes (Example 5).

Example  6

In order to find out whether the photodynamic reaction can be induced in living organisms using the photodynamic reaction induction device according to the present invention, as shown in FIG. 1, the photodynamic reaction induction device according to one embodiment of the present invention is used. The experiment was performed to kill the organism E. coli (gram negative).

Specifically, as a light source, a green LED emitting light energy in the wavelength region of 460 nm to 600 nm was irradiated for 20 minutes at an intensity of 10 mW, and an optical energy wavelength cut filter capable of selectively blocking optical energy wavelengths of less than 480 nm. The light energy wavelength of 480 nm or more among the light energy wavelengths of the green LED was irradiated to the reaction vitreous part. Into the reaction vitreous cell incubator, hematopophyrin (1.34 × 10 ^-4 M), a photosensitive molecule, and Escherichia coli (E. coli, gram negative), which are subjects, were placed, and light can be obtained using light energy of 480 nm or more. An epidemiologic response was induced, thereby killing E. coli.

Example  7 and 8

E. coli was killed in the same manner as in Example 6 except that the light energy irradiation time using the green LED was changed to 30 minutes (Example 7) and 60 minutes (Example 8).

E. coli killing experiments performed in Examples 3 to 8 are summarized in Table 3 below.

division Example 3 4 5 6 7 8 Escherichia coli killing (%) 56.8 100 100 61.7 89.3 100

As shown in Table 3, in Examples 3 to 5, the light activity of the pheophorbide A was expressed by using the light energy of 580 nm or more through the red LED and the light energy wavelength cut filter, and as a result, the light energy irradiation time elapsed. It can be seen that the amount of E. coli killing increases with respect to the number of early E. coli.

In Examples 6 to 8, the light activity of hematopophyrin was expressed by using the light energy of 480 nm or more through the green LED and the light energy wavelength cut filter. In contrast, E. coli killing is increasing.

That is, it can be seen that the photodynamic reaction induction can be performed using the photodynamic reaction induction device according to the present invention.

10: photodynamic reaction induction device
20: light source unit 30: power supply unit
40: light control unit 50: light energy wavelength cut filter
60: reaction initial part 70: pedestal
80: support

Claims (16)

A light source unit installed to radiate light energy to the object;
A power supply unit installed to supply power to the light source unit;
A light control unit installed between the light source unit and the power supply unit to adjust the intensity of light energy radiated from the light source unit; And
And a light energy wavelength cutting filter disposed below the light source unit to selectively block a portion of a wavelength region of the light energy radiated from the light source unit. The method of claim 1,
The light source unit is a photodynamic response inducing device which is an LED or OLED emitting light energy. The method of claim 2,
LED and OLED is a photodynamic reaction induction device that emits light energy having a wavelength of 400 nm to 900 nm. The method of claim 1,
The power supply is a photodynamic reaction induction device that is a portable battery or AC power supply. The method of claim 1,
The optical energy wavelength cut filter is a photodynamic response induction device for selectively blocking the wavelength region according to the wavelength region of the light energy emitted from the light source unit. The method of claim 1,
An optical energy wavelength cut filter is a photodynamic response induction device for selectively blocking light energy having a wavelength of less than 580 nm or selectively blocking light energy having a wavelength of less than 480 nm. The method of claim 1,
A photodynamic reaction induction device further comprising a reaction vitreous portion installed under the optical energy wavelength cut filter to cause the photodynamic reaction. The method of claim 7, wherein
The reaction vitreous part is a photodynamic reaction induction device carrying a photosensitive molecule and an object. The method of claim 8,
Photosensitive molecule is a photodynamic reaction induction apparatus expressing photoactivity by light having a wavelength of 400 nm to 900 nm. The method according to claim 1 or 8,
A device for inducing photodynamic responses, wherein the subject is a chemical or an organism. A photodynamic reaction induction method comprising the step of irradiating light energy to an object containing a photosensitive molecule using the photodynamic reaction induction device according to claim 1. The method of claim 11,
A subject containing a photosensitive molecule is a organism in which the photosensitive molecule is administered. The method of claim 11,
The photosensitive molecule is a photodynamic reaction induction method for expressing photoactivity by light having a wavelength of 400 nm to 900 nm. A method of inducing a photodynamic reaction comprising irradiating light energy to a mixture of photosensitive molecules and an object using the photodynamic reaction induction device according to claim 1. 15. The method of claim 14,
A method of inducing a photodynamic reaction wherein the subject is a chemical or an organism. 15. The method of claim 14,
The sensitizing molecule is a photodynamic reaction induction method by which the optical activity is expressed by light having a wavelength of 400 nm to 900 nm.

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