KR20130020701A - Composition for invigorating gabaa-benzodiazepine receptor and composition having anxiolitic, anti-convulsant, anti-depressant and sleep-improving effect containing brown seaweed extract - Google Patents
Composition for invigorating gabaa-benzodiazepine receptor and composition having anxiolitic, anti-convulsant, anti-depressant and sleep-improving effect containing brown seaweed extract Download PDFInfo
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- KR20130020701A KR20130020701A KR1020130002539A KR20130002539A KR20130020701A KR 20130020701 A KR20130020701 A KR 20130020701A KR 1020130002539 A KR1020130002539 A KR 1020130002539A KR 20130002539 A KR20130002539 A KR 20130002539A KR 20130020701 A KR20130020701 A KR 20130020701A
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- extract
- brown algae
- sleep
- gaba
- anxiety
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Abstract
Description
본 발명은 갈조류 추출물을 유효성분으로 포함하는 GABAA-벤조다이아제핀(benzodiazepine) 수용체 활성용 조성물에 관한 것으로, 더욱 상세하게는 GABAA-벤조다이아제핀 수용체에 친화력을 가져서 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 갖는 조성물에 관한 것이다.The present invention relates to a composition for activating the GABA A -benzodiazepine receptor containing a brown algae extract as an active ingredient, more specifically, having an affinity for the GABA A -benzodiazepine receptor to reduce anxiety, improve spasm, calm It relates to a composition having an action and sleep inducing and improving effects.
현대 사회에서 많은 사람들은 스트레스와 불안, 초조 때문에 수면장애를 겪고 있다. 통계에 의하면 성인의 약 15%가 불안증상에 의한 불면증 때문에 약물치료가 필요하다고 알려져 있으며, 불면의 원인으로는 스트레스, 긴장, 공포 등 다양하며, 치료 약물로는 벤조다이아제핀 계열의 약물과 세로토닌 효능약 등이 사용되고 있으나, 이러한 약물들은 장기간 사용하였을 때 내성 및 의존성이 형성되는 부작용이 심한 문제점이 있다.Many people in modern society suffer from sleep disorders due to stress, anxiety and nervousness. According to statistics, about 15% of adults are in need of drug treatment due to insomnia caused by anxiety symptoms.There are various causes of insomnia such as stress, tension, and fear.Therapeutic drugs include benzodiazepine-based drugs and serotonin efficacy. Drugs and the like are used, but these drugs have a severe problem of side effects of forming resistance and dependence when used for a long time.
따라서, 수면제 장기복용자 및 제한자의 경우 이를 대체할 수 있는 수단으로 천연 보조제의 필요성이 높아지고 있으며, 수면 증진 건강기능식품에 대한 수요가 증대되고 있는 실정이다.Therefore, the necessity of natural supplements as a means to replace the long-term sleepers and limiters of sleeping pills is increasing, the demand for sleep-enhancing health functional food is increasing.
또한, GABAA 수용체는 멤브레인 이온 채널을 형성하는 팬타머릭 단백질로서, 진정, 수면, 불안, 근육긴장, 경련, 기억 상실 등의 조절과 밀접한 관련이 있고, 이를 통하여 GABA(감마-아미노부티르산)이 작용하게 된다. 불안 치료제, 진정제 또는 수면유도제로서 벤조다이아제핀을 포함하여 많은 약물들이 이 수용체에 결합하여 약리작용을 행하고 있고, 벤조다이아제핀 부위에 약물 또는 보조제가 결합하면 GABA에 대한 GABAA 수용체 친화도를 증진시키고 염소 이온의 세포 내 유입을 증가시켜 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 보인다. 그러나, 여전히 약물 의존성을 보이고, 근육완화 및 건망증 등의 심각한 문제를 초래하기도 한다.In addition, GABA A Receptors are phantomic proteins that form membrane ion channels, and are closely related to regulation of sedation, sleep, anxiety, muscle tone, convulsions, memory loss, and the like, thereby allowing GABA (gamma-aminobutyric acid) to act. Many drugs, including benzodiazepines, act as an anxiety, sedative, or sleep-inducing agents by binding to these receptors to perform pharmacological action. When drugs or auxiliaries bind to the benzodiazepine site, GABA A to GABA It enhances receptor affinity and increases the intracellular influx of chlorine ions, showing anxiety relief, spasm improvement, sedation, and sleep induction and improvement. However, they still show drug dependence and cause serious problems such as muscle relaxation and forgetfulness.
따라서, GABAA 수용체의 벤조다이아제핀 사이트에 결합 작용하여 GABAA-벤조다이아제핀 수용체에 친화력을 가지면서 부작용의 위험이 없고 안전한 천연물로부터 얻어진 물질을 이용한 진정-수면 효과의 보조제 개발이 절실히 요구되고 있는 실정이다.Thus, GABA A There is an urgent need to develop an adjuvant for the sedation-sleeping effect using a substance obtained from safe natural products while binding to the benzodiazepine site of the receptor and having an affinity for the GABA A -benzodiazepine receptor.
GABAA 수용체의 벤조다이아제핀 부위에 작용하여 GABAA 수용체가 GABA에 반응하여 염소이온 채널을 활성화시키고, 염소가 세포내로 유입되어 세포막 전위의 과다 분극을 통해서 신경세포성(neuronal) 활동을 늦추는 육상의 허브 및 식물 추출물에 대한 연구가 있으나, 해조류인 갈조류 추출물에 대한 보고는 세계적으로 그 사례가 없었다.GABA A GABA A acts on the benzodiazepine site of the receptor There are studies of terrestrial herb and plant extracts in which receptors activate chlorine ion channels in response to GABA and chlorine enters cells and slows neuronal activity through overpolarization of cell membrane potential. There have been no reports of extracts worldwide.
따라서, 본 발명이 해결하고자 하는 첫 번째 과제는 GABAA 수용체의 벤조다이아제핀 사이트에 결합하여 친화력을 가지는 갈조류 추출물을 포함하는 GABAA-벤조다이아제핀 수용체 활성용 조성물을 제공하는 것이다.Therefore, the first problem to be solved by the present invention is GABA A It provides a composition for activating GABA A -benzodiazepine receptor comprising a brown algae extract having affinity by binding to the benzodiazepine site of the receptor.
본 발명이 해결하고자 하는 두 번째 과제는 갈조류 추출물을 포함하는 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선용 식품 조성물을 제공하는 것이다.The second problem to be solved by the present invention is to provide a food composition for anxiety relief, spasm improvement, sedation, and sleep induction and improvement comprising the brown algae extract.
본 발명은 상기 첫 번째 과제를 달성하기 위하여,The present invention to achieve the first object,
갈조류 추출물을 유효성분으로 하는 GABAA-벤조다이아제핀 수용체 활성용 조성물을 제공한다.It provides a composition for activating GABA A -benzodiazepine receptor with brown algae extract as an active ingredient.
본 발명의 일 실시예에 의하면, 상기 조성물은 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물일 수 있다.According to one embodiment of the invention, the composition may be a pharmaceutical composition for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia.
본 발명의 일 실시예에 의하면, 상기 갈조류는 가시뼈대그물말, 감태, 검둥감태, 곰피, 괭생이모자반, 미역쇠, 바위두둑, 바위수염, 불레기말, 쌍발이모자반, 참가죽그물바탕말, 참그물바탕말, 다시마, 미역 및 패 중에서 선택되는 어느 하나 이상의 갈조류일 수 있다.According to one embodiment of the present invention, the brown alga is a spiny skeletal net, Ecklonia cava, Ecklonia cava, Gompi, Hoe Saeng Mama, Seaweed, Rock Doe, Rock Beard, Fire Horse, Twin Emole, Participation It may be any one or more brown algae selected from sesame seed, seaweed, seaweed and shellfish.
본 발명의 일 실시예에 의하면, 상기 갈조류는 상기 갈조류는 감태(Ecklonia cava) 또는 검둥감태(Ecklonia kurome)일 수 있다.According to one embodiment of the invention, the brown alga is the brown alga is Ecklonia cava ) or the Ecklonia kurome ).
본 발명의 일 실시예에 의하면, 상기 갈조류 추출물은 갈조류 물 추출물, 갈조류 에탄올 추출물, 갈조류 메탄올 추출물, 또는 물, 에탄올 및 메탄올 중에서 선택되는 어느 2 이상의 용매의 혼합용매에 의한 갈조류 추출물일 수 있다.According to one embodiment of the present invention, the brown algae extract may be brown algae water extract, brown algae ethanol extract, brown algae methanol extract, or brown algae extract by a mixed solvent of any two or more solvents selected from water, ethanol and methanol.
본 발명의 일 실시예에 의하면, 갈조류 물 추출물은 40 ~ 100℃의 물로 2 ~ 48시간 동안 갈조류를 추출하여 제조하고, 갈조류 에탄올 추출물은 갈조류를 35 ~ 75%의 에탄올로 20 ~ 60℃에서 2 ~ 36시간 동안 추출하여 제조하며, 상기 갈조류 메탄올 추출물은 갈조류를 35 ~ 85%의 메탄올로 20 ~ 60℃에서 2 ~ 36 시간 동안 추출하여 제조하는 것일 수 있다.According to one embodiment of the present invention, brown algae water extract is prepared by extracting brown algae for 2 to 48 hours with water of 40 ~ 100 ℃, brown algae ethanol extract is 20 ~ 60
상기 갈조류 물 추출물은 셀룰라아제, 비스코자임, 알칼라아제 및 펙신으로 이루어진 군으로부터 선택되는 1종 이상의 효소를 첨가한 물로 40 ~ 55℃에서 2 ~ 24시간 갈조류를 추출하여 제조하는 것일 수 있다.The brown algae water extract may be prepared by extracting brown algae for 2 to 24 hours at 40 to 55 ° C. with water to which at least one enzyme selected from the group consisting of cellulase, biscozyme, alcalase and pepsin is added.
본 발명은 상기 두 번째 과제를 달성하기 위하여,According to another aspect of the present invention,
갈조류 추출물을 유효성분으로 하는 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선용 식품 조성물을 제공한다.Provided is a food composition for alleviating anxiety, improving spasm, sedation, and inducing and improving sleep using brown algae extract as an active ingredient.
본 발명의 일 실시예에 의하면, 상기 갈조류는 가시뼈대그물말, 감태, 검둥감태, 곰피, 괭생이모자반, 미역쇠, 바위두둑, 바위수염, 불레기말, 쌍발이모자반, 참가죽그물바탕말, 참그물바탕말, 다시마, 미역 및 패 중에서 선택되는 어느 하나 이상의 갈조류일 수 있다.According to one embodiment of the present invention, the brown alga is a spiny skeletal net, Ecklonia cava, Ecklonia cava, Gompi, Hoe Saeng Mama, Seaweed, Rock Doe, Rock Beard, Fire Horse, Twin Emole, Participation It may be any one or more brown algae selected from sesame seed, seaweed, seaweed and shellfish.
본 발명의 일 실시예에 의하면, 상기 갈조류는 감태(Ecklonia cava) 또는 검둥감태(Ecklonia kurome)일 수 있다.According to one embodiment of the invention, the brown alga is Ecklonia cava ) or Ecklonia kurome .
본 발명의 일 실시예에 의하면, 상기 갈조류 추출물은 갈조류 물 추출물, 갈조류 에탄올 추출물, 갈조류 메탄올 추출물, 또는 물, 에탄올 및 메탄올 중에서 선택되는 어느 2 이상의 용매의 혼합용매에 의한 갈조류 추출물일 수 있다.According to one embodiment of the present invention, the brown algae extract may be brown algae water extract, brown algae ethanol extract, brown algae methanol extract, or brown algae extract by a mixed solvent of any two or more solvents selected from water, ethanol and methanol.
본 발명의 일 실시예에 의하면, 상기 조성물은 정제, 산제, 과립제 및 캅셀제 중에서 어느 하나의 형태로 제제화 된 건강기능식품일 수 있다.According to one embodiment of the invention, the composition may be a health functional food formulated in any one form of tablets, powders, granules and capsules.
본 발명에 따른 갈조류 추출물은 GABAA-벤조다이아제핀 수용체에 친화력을 가져 이를 포함하는 조성물은 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선에 효과적이고, 천연물로부터 얻어진 물질을 이용하기 때문에 부작용을 유발하지 않고, 안전성을 확보할 수 있으며, 약학 조성물이나 건강기능식품 등의 식품 조성물로도 매우 유용하다.Brown algae extract according to the present invention has affinity for the GABA A -benzodiazepine receptor, the composition comprising it is effective in alleviating anxiety, improving spasm, sedation, and inducing and improving sleep, side effects because it uses a substance obtained from natural products It is possible to secure safety without causing, and is very useful as a food composition such as pharmaceutical composition or health functional food.
도 1은 감태 물 추출물(ECK-W), 감태 메탄올 추출물(ECK-M), 감태 에탄올 추출물(ECK-E) 각각에서, 방사성 표지된 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합억제 효과를 나타낸 그래프이다.
도 2a는 각각의 샘플을 쥐에게 경구투여하고 45분 후 hypnotic(45 mg/kg) 농도의 펜토바비탈을 복강주사로 투여하고 입면시간(sleep latency)을 나타낸 그래프이고, 도 2b는 수면시간(sleep duration)을 나타낸 그래프이다.
*은 대조구와 비교하여 p<0.05에서 유의적 차이가 있고, **은 대조구와 비교하여 p<0.01에서 유의적 차이가 있다.(Dunnet's test)
CON : 대조구(0.5% CMC-saline 10 mL/kg)을 경구투여 후 펜토바비탈 투여
DZP : 디아제팜(diazepamP) 2 mg/kg을 경구투여 후 펜토바비탈 투여
ECK-E : 감태 에탄올 추출물을 각각 100, 250, 500, 1000 mg/kg 투여 후 펜토바비탈 투여
ECK-W : 감태 물 추출물을 각각 100, 250, 500, 1000 mg/kg 투여 후 펜토바비탈 투여
도 3은 감태 에탄올 추출물로부터 용매 분획을 제조하는 과정을 나타낸 개요도이다.
도 4a는 감태 추출물 분획의 방사성 표지된 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합억제 효과를 나타낸 그래프이고, 도 4b는 각각의 감태 추출물 분획을 쥐에게 경구투여하고 45분 후 hypnotic(45 mg/kg) 농도의 펜토바비탈을 복강주사로 투여하고 수면시간(sleep duration)을 나타낸 그래프이다.
도 5a는 감태 추출물 분획의 총페놀함량(TPC)와 [3H] 플루마제닐(flumazenil)의 결합에 대한 IC50의 상관관계를 나타낸 그래프이고, 도 5b는 각 감태 추출물 분획의 총페놀함량(TPC)와 수면시간의 상관관계를 나타낸 그래프이다.
도 6a는 감태 추출물 분획에서 분리된 플로로탄닌 화합물인 플로로글루시놀, 엑콜, 엑스톨로놀, 트리플로레톨-A 및 디엑콜의 구조이고, 도 6b는 퓨코디플로레톨 G, 6,6'-비엑콜, 7-플로로엑콜, 6,8'-비엑콜, 8,8'-비엑콜의 구조이다.
도 7a는 검둥감태 메탄올 추출물, 감태 메탄올 추출물, 가시뼈대그물말 메탄올 추출물 및 참가죽그물바탕말 메탄올 추출물의 총페놀함량(TPC)와 10 mg/mL농도에서의 [3H] 플루마제닐(flumazenil)의 결합 활성의 상관관계를 나타낸 그래프이고, 도 7b는 각 갈조류 메탄올 추출물의 총페놀함량(TPC)와 수면시간의 상관관계를 나타낸 그래프이다.
도 8a는 검둥감태 메탄올 추출물(EKO-M)의 방사성 표지된 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합억제 효과를 나타낸 그래프이고, 도 8b는 검둥감태 메탄올 추출물(EKO-M)을 쥐에게 경구투여하고 45분 후 hypnotic(45 mg/kg) 농도의 펜토바비탈을 복강주사로 투여하고 입면시간(sleep latency)을 나타낸 그래프이고, 도 8c는 검둥감태 메탄올 추출물(EKO-M)을 쥐에게 경구투여하고 45분 후 hypnotic(45 mg/kg) 농도의 펜토바비탈을 복강주사로 투여하고 수면시간(sleep duration)을 조사한 그래프이다.
*은 대조구와 비교하여 p<0.05에서 유의적 차이가 있고, **은 대조구와 비교하여 p<0.01에서 유의적 차이가 있다.(Dunnet's test)
CON : 대조구(0.5% CMC-saline 10 mL/kg)을 경구투여 후 펜토바비탈 투여
DZP : 디아제팜(diazepam) 2 mg/kg을 경구투여 후 펜토바비탈 투여
ECK-M : 감태 메탄올 추출물을 각각 1000 mg/kg 투여 후 펜토바비탈 투여
EKO-M : 검둥감태 메탄올 추출물을 1000 mg/kg 투여 후 펜토바비탈 투여
도 9a 및 도 9b는 감태 에탄올 추출물 및 물 추출물과 다이아제팜의 병용경구투여에 대한 대한 수면유도 시너지 효과를 알아보기 위하여 ECK-E는 100 mg/kg, ECK-W는 100 mg/kg, 디아제팜(diazepam, DZP)은 0.5 mg/kg을 펜토바비탈 투여(hypnotic dosage 45 mg/kg) 45분 전에 경구 투여하여 측정한 입면시간(sleep latency)와 수면시간(sleep duration)을 나타낸 그래프이다.
*은 대조구와 비교하여 p<0.05에서, ***은 대조구와 비교하여 p<0.001에서 유의적 차이가 있음(Dunnet's test)을 의미한다.
#은 p<0.05에서, ##은 p<0.01에서 병용 투여한 그룹과 단일 투여한 그룹 사이의 유의적인 차이가 있다는 것을 의미한다.(Unpaired Student's t-test)
NS는 유의적인 차이가 없다는 것을 의미한다.
도 10a와 도 10b는 플루마제닐(flumazenil, FLU)이 감태 에탄올 추출물, 물 추출물, 에틸아세테이트 분획의 수면유도 효과에 미치는 영향에 대해서 알아보기 위하여 ECK-E는 1000 mg/kg, ECK-W는 1000 mg/kg, EtAcO는 200 mg/kg, 디아제팜(diazepam, DZP)는 0.5 mg/kg을 펜토바비탈 투여(hypnotic dosage 45 mg/kg) 45분 전에 경구 투여하였고, 플루마제닐(flumazenil, FLU)은 8 mg/kg을 ECK-E, ECK-W, DZP 경구 투여 10분전에 미리 복강 주사하여 입면시간(sleep latency)과 수면시간(sleep duration)에 대해서 측정한 그래프이다.
*은 대조구와 비교하여 p<0.05에서, **은 p<0.01에서, ***은 p<0.001에서 유의적 차이가 있음(Dunnet's test)을 의미하고,
#은 p<0.05에서, ##은 p<0.01에서, ###은 p<0.001에서, 플루마제닐을 처리한 그룹과 처리하지 않는 그룹 사이의 유의적인 차이가 있다는 것을 의미한다.(Unpaired Student's t-test)
NS는 유의적인 차이가 없다는 것을 의미한다.
도 11a 및 도 11b는 감태 에탄올 추출물이 쥐의 수면구조에 미치는 영향을 알아보기 위하여 Control군은 0.5% CMC-saline 용액을 ECK-E는 500 mg/kg을 각각 경구투여(p.o.) 하고 뇌파를 측정한 후 분석한 그래프이다.
*은 대조구와 비교하여 p<0.05에서 유의적 차이가 있음(Dunnet's test)을 의미한다.1 shows radiolabeled GABA A -benzodiazepine ligands [ 3 H] flumazenyl (ECK-W), Ecklonia cava extract (ECK-M), Ecklonia ethanol extract (ECK-E), respectively. flumazenil) is a graph showing the binding inhibitory effect.
FIG. 2A is a graph showing sleep latency after pentobarbital with hypnotic (45 mg / kg) concentration and 45 minutes after oral administration of each sample to mice, and FIG. 2B is a sleep time ( graph showing sleep duration).
* Has a significant difference at p <0.05 compared to the control and ** has a significant difference at p <0.01 compared to the control (Dunnet's test).
CON: Pentobarbital after oral administration of the control (0.5% CMC-
DZP: Pentobarbital after oral administration of 2 mg / kg of diazepamP
ECK-E: Pentobarbital after Ecklonia
ECK-W: Pentobarbital after 100, 250, 500 and 1000 mg / kg of Ecklonia cava extract
Figure 3 is a schematic diagram showing a process for preparing a solvent fraction from Ecklonia cava ethanol extract.
Figure 4a is a graph showing the binding inhibitory effect of radiolabeled GABA A -benzodiazepine ligand [ 3 H] flumazenil of Ecklonia cava extract fraction, Figure 4b is oral administration of each Ecklonia cava extract fraction to rats After 45 minutes, pentobarbital at a hypnotic (45 mg / kg) concentration was administered by intraperitoneal injection and shows a sleep duration.
Figure 5a is a graph showing the relationship between the total phenolic content (TPC) and [ 3 H] flumazenil of the Ecklonia cava extract fraction, IC 50 , Figure 5b is the total phenolic content of each Ecklonia cava extract fraction ( TPC) is a graph showing the correlation between sleep time.
Figure 6a is a structure of the phloroglucinol, execol, exotolol, triloretol-A and dieckol separated from the Ecklonia cava extract fraction, Figure 6b is fucodifloretol G, 6,6 It is the structure of '-bieckol, 7-floroeckol, 6,8'-bieckol, 8,8'-bieckol.
FIG. 7A shows the total phenolic content (TPC) and the [ 3 H] flumazenil at a concentration of 10 mg / mL of the black-brown Ecklonia cava extract, Ecklonia cava extract, Spiny skeletal net methanol extract, and P. ) Is a graph showing the correlation between the binding activity, Figure 7b is a graph showing the correlation between the total phenolic content (TPC) and the sleep time of each brown algae methanol extract.
Figure 8a is a graph showing the binding inhibitory effect of the radiolabeled GABA A -benzodiazepine ligand [ 3 H] flumazenil of the black persimmon methanol extract (EKO-M), Figure 8b is a black persimmon methanol extract (EKO-M) 45 minutes after oral administration of EKO-M) to rats, pentobarbital at a hypnotic (45 mg / kg) concentration was administered by intraperitoneal injection, showing the sleep latency (sleep latency). 45 minutes after oral administration of EKO-M) to rats, pentobarbital at a hypnotic (45 mg / kg) concentration was administered by intraperitoneal injection and a graph of sleep duration was examined.
* Has a significant difference at p <0.05 compared to the control and ** has a significant difference at p <0.01 compared to the control (Dunnet's test).
CON: Pentobarbital after oral administration of the control (0.5% CMC-
DZP: Pentobarbital after oral administration of 2 mg / kg of diazepam
ECK-M: Pentobarbital after 1000 mg / kg of Ecklonia cava extract
EKO-M: Administration of pentabarbital after administration of 1000 mg / kg of the black ethanol extract
Figures 9a and 9b is ECK-
* Indicates a significant difference at p <0.05 compared to the control and *** at p <0.001 compared to the control (Dunnet's test).
# Means p <0.05 and ## means there is a significant difference between the co-administered group and the single-dose group at p <0.01 (Unpaired Student's t-test)
NS means no significant difference.
10A and 10B illustrate the effects of flumazenil (FLU) on the sleep induction effect of Ecklonia cava ethanol extract, water extract, and ethyl acetate fraction, and ECK-E is 1000 mg / kg, and ECK-W is 1000 mg / kg,
* Means significant difference at p <0.05, ** at p <0.01, *** at p <0.001 compared to the control (Dunnet's test),
# At p <0.05, ## at p <0.01, ### at p <0.001, meaning there is a significant difference between flumagenyl and untreated groups (Unpaired Student's t-test)
NS means no significant difference.
11a and 11b is a control group in 0.5% CMC-saline solution and ECK-E in 500mg / kg oral administration (po) to determine the effect of Ecklonia cava ethanol extract on the sleep structure of rats, and measured the EEG After analyzing the graph.
* Means significant difference (Dunnet's test) at p <0.05 compared to the control.
이하, 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
GABAA 수용체는 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과와 관련되어 있으며, GABAA 수용체에 작용하는 약물로는 작용제(agonist)로서는 벤조다이아제핀, 디아제팜(diazepam, DZP), 졸피뎀(zolpidem) 등이 있고, 길항제(antagonist)로는 플루마제닐(flumazenil, FLU)이 있다. 상기에서 언급하였듯이 GABAA 수용체에 작용하는 약물의 경우 의존성 및 부작용을 초래하는 문제점이 있다.GABA A Receptors are associated with anxiety relief, spasms, sedation, and sleep induction and improvement effects, GABA A Drugs that act on the receptor include agonists such as benzodiazepine, diazepam (DZP), zolpidem and the like, and flumazenil (FLU) as an antagonist. As mentioned above, in the case of drugs acting on the GABA A receptor, there is a problem that causes dependency and side effects.
갈조류 추출물은 녹조류나 홍조류와 달리 GABAA-benzodiazepine(벤조다이아제핀) 수용체에 작용하여 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 가지고 있는 것을 확인하였다. 상기 수면 개선은 입면시간(sleep latency)가 감소하고, 수면시간(sleep duration)이 증가하는 효과일 수 있으나, 이에 본 발명의 범위가 제한되지 아니한다.Brown algae extract, unlike green algae and red algae, acted on GABA A -benzodiazepine (benzodiazepine) receptors to reduce anxiety, improve spasms, sedation, and induce and improve sleep. The sleep improvement may be an effect of decreasing sleep latency and increasing sleep duration, but the scope of the present invention is not limited thereto.
상기 갈조류는 예를 들어 가시뼈대그물말, 감태, 검둥감태, 곰피, 괭생이모자반, 미역쇠, 바위두둑, 바위수염, 불레기말, 쌍발이모자반, 참가죽그물바탕말, 참그물바탕말, 다시마, 미역 및 패 중에서 선택되는 어느 하나 이상의 갈조류일 수 있고, 바람직하게는 감태(Ecklonia cava) 또는 검둥감태(Ecklonia kurome)이고, 더욱 바람직하게는 감태이다.The brown algae are, for example, spiny skeletal net, Ecklonia cava, black ecstasy, Gompi, Hoksaeng mother's nest, seaweed, rocky thump, rockbeard, fire horse, twin mother's nest, participating porridge, kelp May be any one or more brown algae selected from seaweed and shellfish, preferably Ecklonia cava ) or Ecklonia kurome , and more preferably Ecklonia kurome .
상기 갈조류 추출물은 물, 유기용매 또는 이들의 혼합물을 추출 용매로서 이용하여 추출된 것일 수 있다. 이 때 사용되는 유기용매의 종류나 물과 유기용매의 혼합 비율은 특별히 제한되지 않는다. The brown algae extract may be extracted using water, an organic solvent or a mixture thereof as an extraction solvent. The kind of organic solvent used at this time and the mixing ratio of water and an organic solvent are not specifically limited.
예를 들어, 상기 유기용매는 저급 알코올, 헥산, 아세톤, 에틸 아세테이트, 클로로포름, 및 디에틸에테르로 이루어진 군으로부터 선택된 하나 이상의 용매일 수 있다. 상기 저급 알코올은 탄소수 1 내지 6의 알코올일 수 있다. 예를 들어, 저급 알코올로는 메탄올, 에탄올, 프로판올, 부탄올, 노말-프로판올, 이소-프로판올, 노말-부탄올, 1-펜탄올, 2-부톡시에탄올 또는 에틸렌글리콜 등을 이용할 수 있다. 유기용매는 이 외에도 아세트산, DMFO(dimethyl-formamide), DMSO(dimethyl sulfoxide) 등의 극성 용매, 아세토나이트릴, 에틸 아세테이트, 메틸 아세테이트, 플루오로알칸, 펜탄, 2,2,4-트리메틸펜탄, 데칸, 사이클로헥산, 사이클로펜탄, 디이소부틸렌, 1-펜텐, 1-클로로부탄, 1-클로로펜탄, o-자일렌, 디이소프로필 에테르, 2-클로로프로판, 톨루엔, 1-클로로프로판, 클로로벤젠, 벤젠, 디에틸 에테르, 디에틸 설파이드, 클로로포름, 디클로로메탄, 1,2-디클로로에탄, 어닐린, 디에틸아민, 에테르, 사염화탄소 및 THF(Tetrahydrofuran) 등의 비극성 용매를 사용할 수도 있다.For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol may be used as the lower alcohol. Organic solvents include polar solvents such as acetic acid, dimethyl-formamide (DMFO) and dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, and decane. , Cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene And nonpolar solvents such as benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, anneal, diethylamine, ether, carbon tetrachloride and THF (Tetrahydrofuran).
상기 갈조류 추출물은 갈조류 물 추출물, 갈조류 에탄올 추출물, 갈조류 메탄올 추출물, 또는 물, 에탄올 및 메탄올 중에서 선택되는 어느 2 이상의 용매의 혼합용매에 의한 갈조류 추출물일 수 있으나, 이에 본 발명의 범위가 제한되지 아니한다.The brown algae extract may be brown algae water extract, brown algae ethanol extract, brown algae methanol extract, or brown algae extract by a mixed solvent of any two or more solvents selected from water, ethanol and methanol, but the scope of the present invention is not limited thereto.
물 추출물의 제조는 갈조류를 40 ~ 100℃의 물로 2 ~ 48시간 동안 추출하여 제조할 수 있고, 또한 바람직하게는 물 추출에 따른 수용성 성분의 추출 효율을 증대시키기 위하여 셀룰라아제, 비스코자임, 알칼라아제 및 펙신으로 이루어진 군으로부터 선택되는 1종 이상의 효소를 첨가한 물로 40 ~ 55℃에서 2 ~ 24시간 동안 추출하여 제조할 수 있다.The preparation of water extract may be prepared by extracting brown algae with water at 40 to 100 ° C. for 2 to 48 hours, and preferably, to increase the extraction efficiency of water-soluble components according to water extraction, cellulase, biscozyme, alkalase And it can be prepared by extracting at 40 ~ 55 ℃ for 2 to 24 hours with water added at least one enzyme selected from the group consisting of pepsin.
에탄올 추출물의 제조는 갈조류를 35 ~ 75%의 에탄올로 20 ~ 60℃에서 2 ~ 36 시간, 바람직하게는 40 ~ 50℃에서 2.5 ~ 6 시간 동안 추출하여 제조할 수 있고, 보다 바람직하게는 70%의 에탄올 수용액으로 45℃에서 3시간 동안 추출하여 제조한다.The ethanol extract may be prepared by extracting brown algae with 35 to 75% of ethanol at 20 to 60 ° C. for 2 to 36 hours, preferably at 40 to 50 ° C. for 2.5 to 6 hours, more preferably 70% Prepared by extraction for 3 hours at 45 ℃ with an aqueous ethanol solution.
메탄올 추출물의 제조는 갈조류를 35 ~ 85%의 메탄올로 20 ~ 60℃에서 2 ~ 36 시간 동안 추출하여 제조할 수 있고, 바람직하게는 20 ~ 30℃에서 22 ~ 26 시간 동안 추출하여 제조할 수 있고, 보다 바람직하게는 80% 의 메탄올로 25℃에서 24시간 동안 추출하여 제조한다.The preparation of the methanol extract may be prepared by extracting brown algae with 35 to 85% of methanol at 20 to 60 ° C. for 2 to 36 hours, preferably by extracting at 20 to 30 ° C. for 22 to 26 hours. More preferably, 80% of methanol is extracted at 25 ° C. for 24 hours.
본 발명의 갈조류 추출물 중에서, 감태 물 추출물은 상기 GABAA-벤조다이아제핀 수용체의 결합측정에서 IC50이 1.02 ~ 1.03 ㎛일 수 있다. 또한 감태 에탄올 추출물은 상기 GABAA-벤조다이아제핀 수용체의 결합 측정에서 IC50이 0.45 ~ 0.46 ㎛일 수 있다. 또한 감태 메탄올 추출물은 상기 GABAA-벤조다이아제핀 수용체의 결합 측정에서 IC50이 1.25 ~ 1.26 ㎛일 수 있다. 또한 검둥 감태 메탄올 추출물은 상기 GABAA-벤조다이아제핀 수용체의 결합 측정에서 IC50이 0.62 ~ 0.63 ㎛일 수 있다.In the brown algae extract of the present invention, the Ecklonia cava extract may have an IC 50 of 1.02 to 1.03 μm in the binding measurement of the GABA A -benzodiazepine receptor. In addition, Ecklonia cava ethanol extract may have an IC 50 of 0.45 ~ 0.46 ㎛ in the measurement of binding of the GABA A -benzodiazepine receptor. In addition, the Ecklonia cava extract may have an IC 50 of 1.25 to 1.26 μm in measurement of binding of the GABA A -benzodiazepine receptor. In addition, the black Ecklonia cava extract may have an IC 50 of 0.62 to 0.63 μm in the binding measurement of the GABA A -benzodiazepine receptor.
본 명세서에서 사용되는 용어 ‘추출물’은 추출물을 추가적으로 분획(fractionation)한 분획물도 포함한다. 즉, 갈조류 추출물은 상술한 추출용매를 이용하여 얻은 것뿐만 아니라, 여기에 정제과정을 추가적으로 적용하여 플로로탄닌을 농축시켜 얻은 것도 포함한다. 또한, 상기 추출물이나 분획물을 일정한 분자량 컷-오프 값을 갖는 한외 여과막을 통과시켜 얻은 분획, 다양한 크로마토그래피 (크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 갈조류 추출물에 포함된다.As used herein, the term 'extract' also includes fractions that additionally fractionate the extract. That is, the brown algae extract includes not only one obtained by using the above-described extraction solvent, but also one obtained by concentrating phlorotannin by additionally applying a purification process thereto. In addition, fractions obtained by passing the extract or fraction through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the various purification methods described above are also included in the brown seaweed extract of the present invention.
본 발명의 갈조류 추출물을 유효성분으로 하는 GABAA-벤조다이아제핀 수용체 활성용 조성물은 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물일 수 있다. GABA A -benzodiazepine receptor active composition comprising the brown algae extract of the present invention as an active ingredient may be a pharmaceutical composition for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia.
상기 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물의 약학적 투여 형태는 이들의 약학적 허용 가능한 염의 형태로 사용할 수 있고, 단독으로 타 약학적 활성 화합물과 결합뿐만 아니라 적당한 집합의 형태로 사용할 수 있다. Pharmaceutical dosage forms of the pharmaceutical compositions for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia can be used in the form of their pharmaceutically acceptable salts, and can be used alone or in combination with other pharmaceutically active compounds. Can be used in the form of a set.
또한 본 발명에 따른 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물은 각각 통상의 방법에 따라 산제, 과립제, 저제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구제 제형, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화되어 사용할 수 있고, 제형화를 위하여 약학 조성물의 제조에 통상적으로 사용되는 적절한 담체, 부형제 또는 희석제를 포함할 수 있다.In addition, the pharmaceutical composition for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia according to the present invention is oral preparations such as powders, granules, capsules, capsules, suspensions, emulsions, syrups, aerosols, etc. It can be formulated and used in the form of formulations, external preparations, suppositories, and sterile injectable solutions, and can include suitable carriers, excipients or diluents commonly used in the manufacture of pharmaceutical compositions for formulation.
상기 담체 또는, 부형제 또는 희석제로는 락토즈, 덱스트로즈, 수크로오스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리게이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유 등을 포함한 다양한 화합물 혹은 혼합물을 들 수 있다.The carrier or excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicide, cellulose, methyl cellulose, undetermined. And various compounds or mixtures including vaginal cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like.
제제화할 경우에는 보통 사용하는 충진제, 중량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 제조할 수 있다.When formulated, it may be prepared using diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, and surfactants that are commonly used.
경구 투여를 위한 고형제제는 상기 추출물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘보네이트, 수크로스 또는 락토오스, 젤라틴 등을 섞어 제조할 수 있다. 또한 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용할 수 있다.Solid preparations for oral administration may be prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin and the like in the extract. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.
경구를 위한 액상 제제로는 현탁액, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용하는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등을 포함할 수 있다.Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, preservatives, etc., in addition to commonly used simple diluents such as water and liquid paraffin. .
비경구 투여를 위한 제제에는 멸균된 수용액, 비수용성제, 현탁제, 유제, 동결건조 제제, 좌제가 포함된다. 비수성용제, 현탁제로는 프로필렌글리콜, 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등을 사용할 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세롤젤라틴 등을 사용할 수 있다.Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerol gelatin and the like can be used.
본 발명에 따른 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물의 바람직한 투여량은 환자의 상태, 체중, 질병의 정도, 약물형태, 투여경로 및 기간에 따라 다르지만, 당업자에 의해 적절하게 선택될 수 있다. 그러나, 바람직한 효과를 위해서는 1일 0.0001 내지 2,000 mg/kg으로, 바람직하게는 0.001 내지 2,000 mg/kg으로 투여할 수 있다. 투여는 하루에 한 번 투여할 수도 있고, 수회 나누어서 투여할 수도 있다. 다만, 상기 투여량에 의해서 본 발명의 범위를 한정하는 것은 아니다.Preferred dosages of the pharmaceutical compositions for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia according to the present invention vary depending on the condition, weight, extent of disease, drug form, route of administration and duration of the patient. Can be appropriately selected. However, for the desired effect, it may be administered at 0.0001 to 2,000 mg / kg, preferably at 0.001 to 2,000 mg / kg. Administration may be once a day or may be divided several times. However, the scope of the present invention is not limited by the above dosage.
본 발명에 따른 항불안, 항경련(anti-convulsant), 진정 및 불면증 치료용 약학 조성물은 쥐, 생쥐, 가축, 인간 등의 포유 동물에 다양한 경로로 투여할 수 있다. 투여의 모든 방식은 예를 들면, 경구, 직장 또는 정맥, 근육, 피하, 자궁 내 경막 또는 뇌혈관내(intracerebroventricular) 주사에 의해서 투여할 수 있다.
The pharmaceutical composition for the treatment of anti-anxiety, anti-convulsant, sedation and insomnia according to the present invention can be administered to mammals such as rats, mice, livestock, humans by various routes. All modes of administration may be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.
또한, 본 발명은 갈조류 추출물을 유효성분으로 하는 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선용 식품 조성물인 것을 특징으로 한다.In addition, the present invention is characterized by a food composition for relief of anxiety, spasm improvement, sedation, and sleep induction and improvement using the brown algae extract as an active ingredient.
본 발명에 따른 추출물을 건강기능식품 또는 일반 식품의 유효성분 첨가물로 사용하는 경우 본 발명에 따른 추출물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용할 수 있고, 통상적인 방법에 따라 적절하게 사용할 수 있다. 유효 성분의 혼합양은 예방, 건강 또는 치료 등의 각 사용 목적에 따라 적합하게 결정할 수 있다.When the extract according to the present invention is used as an active ingredient additive of a health functional food or a general food, the extract according to the present invention may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. . The mixed amount of the active ingredient can be appropriately determined depending on the purpose of use, such as prevention, health or treatment.
일반적으로, 식품 또는 음료의 제조시에 본 발명에 따른 추출물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가할 수 있다. 그러나, 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 양은 상기 범위 이하일 수 있으며, 또한 본 발명은 천연물로부터의 추출물을 이용하는 점에서 안전성 면에서 문제가 없으므로 상기 범위 이상의 양으로도 사용할 수 있다.In general, the extract according to the invention in the preparation of food or beverage may be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the present invention has no problem in terms of safety in terms of using an extract from natural products. The above amount can also be used.
상기 식품의 종류에는 특별히 제한은 없고, 상기 물질을 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 쵸콜렛, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 식품을 모두 포함할 수 있다.There is no restriction | limiting in particular in the kind of said food, The foodstuff which can add the said substance is a dairy product including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, etc. , Various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, etc., may include all foods in a conventional sense.
본 발명에 따른 건강기능식품 중 음료 식품은 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜일 수 있다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명에 따른 기능성 식품 100 mL당 약 0.01 ~ 0.04 g, 바람직하게는 약 0.02 ~ 0.03 g일 수 있다.The beverage food of the health functional food according to the present invention may contain various flavors or natural carbohydrates as an additional ingredient, as in general drinks. The above-mentioned natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate may be about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL functional food according to the present invention.
상기 외에 본 발명에 따른 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선용 식품 조성물은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제를 함유할 수 있다. 그 밖에 본 발명의 수면 개선용 조성물은 천연 과일쥬스, 과일쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 혼합하여 사용할 수 있다. 이러한 첨가제의 비율은 제한되지 않으나 본 발명의 조성물 100중량부 대비 0.01 ~ 0.1 중량부의 범위에서 선택되는 것이 일반적이다.
In addition to the above, the food composition for anxiety relief, convulsion improvement, sedation, and sleep induction and improvement according to the present invention are various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, Protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages. In addition, the composition for improving sleep of the present invention may contain fruit flesh for the production of natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The ratio of such additives is not limited but is generally selected in the range of 0.01 to 0.1 parts by weight relative to 100 parts by weight of the composition of the present invention.
이하, 바람직한 실시예를 들어 본 발명을 더욱 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이에 의하여 제한되지 않는다는 것은 당업계의 통상의 지식을 가진 자에게 자명할 것이다.
Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. It will be apparent, however, to those skilled in the art that these embodiments are for further explanation of the present invention and that the scope of the present invention is not limited thereby.
실험예Experimental Example 1: 해조류 추출물에서의 1: in seaweed extract GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 탐색 Exploring Receptor Binding Activity
불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 갖는 해조류를 탐색하기 위하여 5종의 녹조류, 7종의 홍조류 및 6종의 갈조류, 총 18종의 해조류의 메탄올 추출물을 제주테크노파크의 제주 생물자원 추출물 은행에서 구입하여 GABAA-벤조다이아제핀 수용체 결합력을 측정하였다. 증거 견본(Voucher specimen)은 제주 생물자원 추출물 은행에 기탁하였다.In order to search for algae with anxiety relief, convulsion improvement, sedation, and sleep induction and improvement effects, methanol extracts of five kinds of green algae, seven red algae and six brown algae and a total of 18 algae were prepared in Jeju Techno Park. Purchased from the Jeju Biomass Extract Bank, GABA A -benzodiazepine receptor binding capacity was measured. Voucher specimens were deposited at the Jeju Biomass Extract Bank.
GABAA-벤조다이아제핀 수용체 결합 활성 측정은 SD rat의 대뇌피질을 적출한 후 즉시 30 mM Tris-HCl buffer (pH 7.4, keep at 4) 20 mL에 넣어 균질화시키고 10초 동안 초음파를 처리하였다. 이 후 27,000×g 및 4℃의 조건에서 15분간 원심분리한 후 상층액을 버리고 다시 buffer 20 mL을 취하여 원심분리하였으며, 이 과정을 3번 반복하였다. 뇌 조직 안의 GABA를 제거하기 위하여 37℃의 water bath에서 30분간 incubation 시키고 원심분리한 후 pellet을 수집하여 -80℃에서 동결보관하여 사용하였다. 동결된 membrane을 해동시킨 후 27,000×g 및 4℃의 조건에서 10분간 원심분리한 후 상층액을 버리고 50 mM Tris-citrate buffer (binding buffer, pH 7.1, keep at 4℃) 20 mL을 넣고 원심분리하였다. 이 과정을 3번 반복한 후 membrane을 binding buffer에 500 mL buffer/original tissue g의 농도로 현탁시켜 결합력 평가 시험에 사용하였다. 96-well plate에 membrane suspension 180 ㎕, 각각의 추출물 시료 10 ㎕ 및 [3H] flumazenil (1 nM, final concentration) (Ro 15-1788; PerkinElmer Life and Analytical Sciences, Waltham, MA, USA) 10 ㎕을 넣고 ice-bath에서 40분간 incubation 하였다. 이 후 glass fiber filter (GF/C, Whatman)을 이용하여 수집하였다. 샘플의 radioactivity는 Tri-Carb Liquid Scintillation Analyzers (Perkin-Elmer, Shelton, CT, USA)를 통해 측정되었다. 비특이적 결합 (nonspecific binding, NSB)은 clonazepam (1 uM, final concentration)을 이용하여 그 값을 결정하였고, Binding displacement 값 (%)은 아래 식(1)과 같이 계산하였다.GABA A -benzodiazepine receptor binding activity was measured and homogenized in 20 mL of 30 mM Tris-HCl buffer (pH 7.4, keep at 4) homogenized immediately after the cerebral cortex of SD rats were removed. After centrifugation for 15 minutes at 27,000 × g and 4 ℃ conditions, the supernatant was discarded and centrifuged again with 20 mL of buffer, this process was repeated three times. In order to remove GABA in the brain tissues, the pellets were incubated in a water bath at 37 ° C. for 30 minutes, centrifuged, and pellets were collected and used at -80 ° C. for freezing. After thawing the frozen membrane, centrifuge for 10 minutes at 27,000 × g and 4 ℃, discard the supernatant, add 20 mL of 50 mM Tris-citrate buffer (binding buffer, pH 7.1, keep at 4 ℃), and centrifuge. It was. After repeating this process three times, the membrane was suspended in binding buffer at a concentration of 500 mL buffer / original tissue g and used for the evaluation of binding strength. Into a 96-well plate, add 180 µl of membrane suspension, 10 µl of each extract sample, and 10 µl of [3H] flumazenil (1 nM, final concentration) (Ro 15-1788; PerkinElmer Life and Analytical Sciences, Waltham, MA, USA). Incubation for 40 minutes in ice-bath. Thereafter, the glass fibers were collected using a filter (GF / C, Whatman). Radioactivity of the samples was measured by Tri-Carb Liquid Scintillation Analyzers (Perkin-Elmer, Shelton, CT, USA). Nonspecific binding (NSB) was determined using clonazepam (1 uM, final concentration), and the binding displacement value (%) was calculated as in Equation (1) below.
식(1)Formula (1)
Binding displacement(%)=[1 - ((시료의 DPM - NSB DPM)/(TB DPM - NSB DPM))]×100Binding displacement (%) = [1-((DPM-NSB DPM) / (TB DPM-NSB DPM))] × 100
(DPM: disintegrations per minute, TB: total binding, NSB: nonspecific binding)(DPM: disintegrations per minute, TB: total binding, NSB: nonspecific binding)
추출물의 농도를 각각 0.1 mg/mL, 1 mg/mL 및 10 mg/mL로 달리하여 녹조류 메탄올 추출물의 GABAA-벤조다이아제핀 수용체 결합 활성 결과는 [표 1]에 나타내었고, 홍조류 메탄올 추출물의 결과는 [표 2]에 나타내었으며, 갈조류 메탄올 추출물의 결과는 [표 3]에 나타내었다.The results of GABA A -benzodiazepine receptor binding activity of green algae methanol extracts were shown in [Table 1] by varying the concentrations of the extracts at 0.1 mg / mL, 1 mg / mL and 10 mg / mL, respectively. Are shown in [Table 2], and the results of the brown algae methanol extract are shown in [Table 3].
Codiaceae
Gigartinaceae
Grateloupiaceae
to the GABAA-BZD receptorDisplacement (%) of [ 3 H] -flumazenil binding
to the GABA A -BZD receptor
Sargassaceae
Sargassacea
녹조류에서 모란갈파래(Ulva conglobata Kjellman)와 홍조류에서 꼬시래기(Gracilaria verrucosa Papenfuss)가 10 mg/mL 농도에서 80% 이상의 [3H]-플루마제닐의 결합을 치환하는 GABAA-벤조다이아제핀 수용체 결합 활성을 나타내었으나, 나머지 대부분의 녹조류와 홍조류에서는 효과적인 GABAA-벤조다이아제핀 수용체 결합 활성을 나타내지 못하였다. 그러나 갈조류에서는 참가죽그물바탕말(Dictyota coriacea) 및 쌍발이모자반(Sargassum patens C.Agardh)을 제외하고는 대부분 결합 활성을 10 mg/mL 농도에서 55% 이상의 GABAA-벤조다이아제핀 수용체 결합 활성을 나타내었고, 특히 감태(Ecklonia cava Kjellman)는 90% 이상의GABAA-벤조다이아제핀 수용체 결합 활성을 나타내었다.
Ulva conglobata Kjellman in green algae and Gracilaria in red algae verrucosa Eoteuna exhibit benzodiazepine receptor binding activity, in the rest of the majority of green algae and red algae effective GABA A - - Papenfuss) is 10 mg / mL concentration of 80% or more [3 H] in-fluorene horseshoe carbonyl bond GABA A replacing the benzo It did not show diazepine receptor binding activity. However, in brown algae, Dictyota coriacea and Sargassum patens Except for C.Agardh), most of the binding activity showed more than 55% GABA A -benzodiazepine receptor binding activity at a concentration of 10 mg / mL, especially Ecklonia cava Kjellman showed more than 90% GABA A -benzodiaea. Zepin receptor binding activity was shown.
실험예Experimental Example 2: 추출용매에 따른 2: according to the extraction solvent 감태Ecklonia 추출물의 Extract GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 Receptor binding activity
실험예 1에서 90% 이상의GABAA-벤조다이아제핀 수용체 결합 활성이 가장 뛰어났던 감태(Ecklonia cava Kjellman, ECK)에서 추출용매를 달리 했을 때 활성의 차이가 있는지 확인하기 위하여 추출용매를 달리한 감태 추출물을 제조하였다. Ecklonia cava Kjellman, ECK ( Ecklonia cava Kjellman, ECK), which had the highest GABA A -benzodiazepine receptor binding activity in Experimental Example 1, was used to determine whether there was a difference in activity. Was prepared.
감태 에탄올 추출물은 70% 에탄올로 50℃에서 3시간 동안 추출하였고, 감태 물 추출물은 효소(비스코자임, 알칼라아제, 펙신)를 첨가하여 45℃에서 3시간 동안 추출하였으며, 감태 메탄올 추출물은 80% 메탄올로 상온에서 24시간 추출하였다. 모든 추출물들은 추출 후 여과 및 농축과정을 거친 후 동결건조하여 분말화하였다.Ecklonia cava ethanol extract was extracted with 70% ethanol for 3 hours at 50 ℃, Ecklonia cava water extract was extracted for 3 hours at 45 ℃ with the addition of enzymes (biscozyme, alcalase, pepsin), Ecklonia cava extract 80% Extracted with methanol at room temperature for 24 hours. All extracts were filtered and concentrated, followed by lyophilization and powdering.
도 1은 감태 물, 에탄올, 메탄올 추출물의 방사성 표지된 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐의 결합 치환 효과를 나타낸 그래프이다. 이는 수면효과를 나타내는 성분들을 탐색하기 위하여 일반적으로 사용되는 방법으로 [3H] 플루마제닐의 치환 효과가 높을수록 그 물질의 결합 활성이 높은 것을 의미한다.1 is a graph showing the binding substitution effect of radiolabeled GABA A -benzodiazepine ligand [ 3 H] flumagenyl of Ecklonia cava, ethanol, methanol extract. This is a method generally used to search for components exhibiting sleep effects. The higher the substitution effect of [ 3 H] flumagenyl, the higher the binding activity of the substance.
감태의 3가지 추출물 모두 GABAA-벤조다이아제핀 수용체에 대한 친화력이 농도의존적으로 높아졌으며, IC50 값은 ECK-W(물 추출물)=1.026, ECK-M(메탄올 추출물)=1.256, ECK-E(에탄올 추출물)=0.456 mg/mL을 나타내었다.
All three extracts of Ecklonia cava increased concentration-dependent affinity for GABA A -benzodiazepine receptor, and IC 50 values were ECK-W (water extract) = 1.026, ECK-M (methanol extract) = 1.256, ECK-E. (Ethanol extract) = 0.456 mg / mL.
실험예Experimental Example 3: 추출용매 및 농도에 따른 에 따른 3: depending on the extraction solvent and concentration 감태Ecklonia 추출물의 수면 유도 효과 Sleep Inducing Effect of Extracts
1) 실험재료1) Experimental material
펜토바비탈(Pentobarbital)은 한림 제약회사에서 구입하였고, GABAA-벤조다이아제핀 수용체의 길항제인 디아제팜(diazepam, DZP)은 잘 알려진 수면제 중의 하나로서 진정 및 수면 유도 효과의 양성 대조군으로 사용하였다.Pentobarbital was purchased from Hallym Pharmaceutical Company, and diazepam (DZP), an antagonist of GABA A -benzodiazepine receptor, was used as a positive control of sedative and sleep inducing effects.
실험동물로 무게 18 ~ 22g의 수컷 ICR 생쥐(Koatech Animal사)를 온도 24℃, 습도 55%, 형광등 명암 12시간 사이클(조명 오전 8시)의 조건하에서 시료 및 음수는 자유롭게 섭취하게 하고, 새로운 환경에 적응시키기 위하여 실험 전 1주일간 사육하였다. 또한, GABAA-벤조다이아제핀 수용체 결합 평가를 위한 멤브레인 준비를 위하여 무게 200~250g의 SD 쥐를 사용하였다. 동물 실험과 관련된 모든 절차는 한국식품연구원 동물관리위원회(KFRI-IACUC, Korea Food Research Institutional Animal Care and Use Committee)의 실험동물 사용 지침에 의거 수행하였다.
As a test animal, male ICR mice (Koatech Animal) weighing 18 to 22 g were allowed to ingest samples and negative water freely under conditions of temperature of 24 ° C., humidity of 55%, and fluorescent light and dark 12 hours cycle (8 AM lighting). The animals were bred for one week before the experiment. In addition, SD mice weighing 200-250 g were used for membrane preparation for evaluation of GABA A -benzodiazepine receptor binding. All procedures related to animal testing were performed in accordance with the guidelines for the use of laboratory animals by the Korea Food Research Institutional Animal Care and Use Committee (KFRI-IACUC).
2) 실험방법2) Experiment Method
모든 실험은 오후 1시에서 5시 사이에 진행되었고, 생쥐들은 실험 전 24시간 전에 절식시켰다. 경구 투여를 위하여 감태 에탄올 추출물을 0.5% carboxymethyl cellulose (Control-0.5%, CMC-saline 10 mL/kg)에 희석시키고, 양성 대조군인 디아제팜(diazepam, DZP)은 역시 0.5% CMC-saline에 희석시켰다. 감태 에탄올 추출물 및 물 추출물(각각 100, 250, 500, 1000 mg/kg)과 디아제팜(diazepam, DZP, 2 mg/kg)을 펜토바비탈 투여 45분 전에 경구 투여하였다. 또한 음성 대조군(Control group, CON)로서는 별도의 약물을 투여하지 않고, 0.5% CMC-saline 10 mL/kg을 투여하였다.All experiments took place between 1 and 5 pm and mice were fasted 24 hours before the experiment. For oral administration, Ecklonia cava ethanol extract was diluted in 0.5% carboxymethyl cellulose (Control-0.5%, CMC-
각각 생쥐에게 펜토바비탈(sub-hypnotic dosage 30 mg/kg, hypnotic dosage 45 mg/kg)을 복강 주사한 후 입면시간(sleep latency)과 수면시간(sleep duration)을 측정하였다. 펜토바비탈을 생쥐에게 복강 내에 주사한 뒤로부터 등으로 바닥에 기대어 있다가 두위반사를 상실할 때까지 경과시간을 즉, 펜토바비탈 주사 후 수면개시(sleep onset)까지의 시간을 입면시간(sleep latency)라고 간주하였고, 상자 안의 생쥐 중에서 펜토바비탈 투여 후에 1분 이상 움직임이 없는 경우 잠든 것으로 간주하였다. 수면시간(sleep duration)은 생쥐가 상자가 옮겨진 후 움직임이 없을 때부터 자율행동을 회복하기까지의 시간으로 간주하였다. sub-hypnotic dosage of pentobarbital-treated 실험에서 수면개시 계산 방법은 아래 식(2)와 같다.The mice were intraperitoneally injected with pentobarbital (
식(2)Formula (2)
수면개시(sleep onset, %)=(No.falling asleep)/(Total No.)×100
Sleep onset (%) = (No.falling asleep) / (Total No.) × 100
3) Hypnotic 농도의 펜토바비탈에 의해 유도된 쥐에서 감태 에탄올 및 물 추출물의 수면 유도 효과3) Sleep Induction Effects of Ecklonia cava Ethanol and Water Extracts in Hypnotic Concentrated Pentobarbital Rats
감태 에탄올 추출물(ECK-E) 및 물 추출물(ECK-W)을 100, 250, 500, 1,000 mg/kg의 농도로 경구투여(p.o.)한 후 펜토바비탈(45 mg/kg, i.p.)에 의한 수면유도효과에 미치는 영향을 하기 도 2a 및 도 2b에 나타내었다. 각 그래프는 평균값(means±SEM, n=10)을 나타낸다.Ecklonia cava ethanol extract (ECK-E) and water extract (ECK-W) were orally administered (po) at concentrations of 100, 250, 500, and 1,000 mg / kg, followed by pentobarbital (45 mg / kg, ip). Influence on the sleep induction effect is shown in Figures 2a and 2b. Each graph shows the mean value (means ± SEM, n = 10).
*은 대조구와 비교하여 p<0.05에서, ***은 대조구와 비교하여 p<0.001에서 유의적 차이가 있음(Dunnet's test)을 의미하고, #은 p<0.05에서, ##은 p<0.01에서 병용 투여한 그룹과 단일 투여한 그룹 사이의 유의적인 차이가 있다는 것을 의미한다.* Indicates a significant difference at p <0.05 compared to the control, *** at p <0.001 compared to the control (Dunnet's test), # at p <0.05, ## at p <0.01 It means that there is a significant difference between the group administered in combination and the group administered in single.
감태 에탄올 추출물(ECK-E)은 용량 의존적으로 입면시간을 감소시켰으며, 100 mg/kg을 제외한 농도에서 용량 의존적으로 수면시간을 증가시켜 수면증진 효과를 나타내었다. 감태 물 추출물(ECK-W)의 경우는 낮은 농도에서는 수면효과가 나타나지 않았지만, 500, 1,000 mg/kg의 농도에서 용량 의존적으로 수면시간(sleep duration)의 유의적인 증가(p<0.01)와 입면시간(sleep latency)의 유의적인 감소(p<0.01)가 나타났다. 특히 감태 물 추출물(ECK-W) 1,000 mg/kg의 농도에서는 감태 에탄올 추출물(ECK-E)과 positive control인 DZP보다 유의적으로 감소(p<0.01)한 입면시간(sleep latency)을 확인할 수 있었다.
Ecklonia cava ethanol extract (ECK-E) decreased the dose-dependent elevation and showed a sleep-promoting effect by increasing the dose-dependent sleep time at concentrations other than 100 mg / kg. Ecklonia cava water extract (ECK-W) did not show any sleep effect at low concentrations, but significantly increased dose-dependent sleep duration (p <0.01) and elevation time at concentrations of 500 and 1,000 mg / kg. (sleep latency) showed a significant decrease (p <0.01). In particular, at the concentration of 1,000 mg / kg Ecklonia cava extract (ECK-W), sleep latency was significantly reduced (p <0.01) than Ecklonia cava ethanol extract (ECK-E) and positive control DZP. .
4) Sub-hypnotic 농도의 펜토바비탈에 의해 유도된 쥐에서 감태 에탄올 및 물 추출물의 수면 유도 효과4) Sleep Induction Effect of Ecklonia cava Ethanol and Water Extract in Rats Induced by Sub-hypnotic Concentration of Pentobarbital
감태 에탄올 및 물추출물이 sub-hypnotic (펜토바비탈 30 mg/kg, 복강주사)농도에서의 입면시간(sleep latency)과 수면시간(sleep duration)을 측정하여 수면유도효과에 미치는 영향을 하기 [표 4]과 [표 5]에 나타내었다. 실험결과, 감태에탄올 추출물은 sub-hypnotic 농도의 펜토바비탈에서 대조 그룹은 수면을 유도하지 못했지만, 다이아제팜(2 mg/kg, 경구투여)은 수면 개시가 100%로 가장 높게 측정되었으며 모두가 수면상태로 들었다. 감태 에탄올 및 물추출물에서는 농도 의존적으로 수면개시(sleep oneset)의 입면시간(sleep latency) 증가와 수면시간(sleep duration)의 증가가 나타났으며, 특히, 1000 mg/kg의 농도에서는 수면개시가 각각 92%와 90%로 높게 나타났다. 수면시간(sleep duration)도 각각 38.7±6.3 분과 39.93±4.7 분으로 유의적인 증가(p<0.05)를 나타냈다.Effects of Ecklonia cava ethanol and water extract on sleep induction effect by measuring sleep latency and sleep duration at sub-hypnotic concentrations (
상기 [표 4]과 [표 5]는 CON(0.5%, CMC-saline 10 mL/kg), 감태 에탄올 추출물(ECK-E, 각각 100, 250, 500, 1000 mg/kg), 감태 물 추출물(ECK-W, 각각 100, 250, 500, 1000 mg/kg)과 DZP(2 mg/kg)를 펜토바비탈 투여 45분 전에 경구 투여한 결과로서,[Table 4] and [Table 5] are CON (0.5%, CMC-
수면개시(sleep onset, %) = (수면상태 수/전체 수)×100Sleep onset (%) = (sleep state / total) × 100
수면시간(sleep duration)은 평균값(mean±SEM, n=12)Sleep duration is mean (mean ± SEM, n = 12)
*은 대조구와 비교하여 p<0.05에서 유의적 차이가 있고, **은 대조구와 비교하여 p<0.01에서 유의적 차이가 있다.(Dunnet's test).* Has a significant difference at p <0.05 compared to the control, ** has a significant difference at p <0.01 compared to the control (Dunnet's test).
CON : 대조군 (0.5% CMC-saline 10 mL/kg)을 경구투여 후 펜토바비탈 투여CON: Pentobarbital after oral administration of the control group (0.5% CMC-
DZP : 다이아제팜(diazepam) 2 mg/kg을 경구투여 후 펜토바비탈 투여DZP: Pentobarbital after oral administration of 2 mg / kg of diazepam
ECK-E : 감태 에탄올 추출물 100, 250, 500, 1000 mg/kg을 경구투여 후 펜토바비탈 투여ECK-E: Pentobarbital after oral administration of Ecklonia
ECK-W : 감태 물 추출물 100, 250, 500, 1000 mg/kg을 경구투여 후 펜토바비탈 투여ECK-W: Pentobarbital after oral administration of
상기 [표 4] 및 [표 5]에서와 보는 바와 같이, 감태 에탄올 및 물 추출물은 농도 의존적으로 입면시간의 증가와 수면시간의 증가가 있음을 확인할 수 있다.
As shown in [Table 4] and [Table 5], Ecklonia cava ethanol and water extracts can be confirmed that there is an increase in the elevation time and sleep time depending on the concentration.
실험예Experimental Example 4: 4: 감태Ecklonia 추출물 분획의 Extract fractions GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 및 수면 유도 효과 Receptor binding activity and sleep induction effect
1) 감태 추출물 분획의 제조1) Preparation of Ecklonia cava Extract Fraction
GABAA-벤조다이아제핀 수용체에 결합하여 진정 및 수면 유도 등의 활성을 나타내는 유효성분을 확인하기 위하여 도 3에 나타낸 바와 같이 n-헥산, 에틸아세테이트, n-부탄올 및 물 분획을 제조하였다.
N-hexane, ethyl acetate, n-butanol and water fractions were prepared as shown in FIG. 3 in order to identify the active ingredients that bind to GABA A -benzodiazepine receptor and exhibit activities such as sedation and sleep induction.
2) GABAA-벤조다이아제핀 수용체 결합 활성 및 수면 유도 효과 실험결과2) GABA A -benzodiazepine receptor binding activity and sleep induction effect test results
GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합 치환 효과는 실험예 1의 방법으로 확인하여, 각각의 감태 추출물 분획의 [3H] 플루마제닐(flumazenil)의 결합 치환 효과를 도 4a에 나타내었고, Hypnotic 농도의 펜토바비탈에 의해 유도된 쥐에서 감태 추출물 분획의 수면 유도 효과를 실험예 3의 방법으로 확인하여 도 4b에 나타내었다.The binding substitution effect of GABA A -benzodiazepine ligand [ 3 H] flumazenil was confirmed by the method of Experimental Example 1, and the binding substitution of [ 3 H] flumazenil of each Ecklonia cava extract fraction. The effect is shown in FIG. 4a, and the sleep inducing effect of the Ecklonia cava extract fraction in rats induced by the hypnotic concentration of pentobarbital is shown in FIG. 4b.
에틸아세테이트(EtOAc) 분획이 가장 낮은 IC50 값(0.0185 mg/mL)을 나타내었고, n-부탄올, n-헥산 및 물 분획은 IC50 값이 각각 0.1033, 0.1405 및 0.9607을 나타내었다(도 4a). The ethyl acetate (EtOAc) fraction showed the lowest IC 50 value (0.0185 mg / mL), and the n-butanol, n-hexane and water fractions had IC 50 values of 0.1033, 0.1405 and 0.9607, respectively (FIG. 4A). .
수면 유도 효과와 관련해서도 상기 [3H] 플루마제닐(flumazenil)의 결합 치환 활성과 유사한 경향을 나타내었다. 에틸아세테이트 분획은 100 및 200 mg/mL 모두에서 수면시간이 현저히 증가하였고, 물 분획은 200 mg/mL에서도 의미있는 수면 유도 활성을 나타내지 못하였다(도 4b).Regarding the sleep inducing effect, the [ 3 H] flumazenil showed a similar tendency to the binding substitution activity. The ethyl acetate fraction significantly increased the sleep time at both 100 and 200 mg / mL, and the water fraction did not show significant sleep inducing activity even at 200 mg / mL (FIG. 4B).
상기 결과로부터 감태 추출물의 에틸아세테이트 분획에 GABAA-벤조다이아제핀 수용체에 친화력을 가져서 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 나타내는 유효성분 함량이 많은 것으로 판단되었다.
From the above results, it was determined that the ethyl acetate fraction of Ecklonia cava extract has an affinity for the GABA A -benzodiazepine receptor, which has a high content of an active ingredient that shows anxiety relief, convulsion improvement, sedation, and sleep induction and improvement.
실험예Experimental Example 5: 5: 감태Ecklonia 추출물 분획의Extract fractions 총페놀함량과Total phenolic content and GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 및 수면 유도 효과의 상관관계 Correlation of Receptor Binding Activity and Sleep Induction Effect
감태 추출물의 에틸아세테이트 분획에는 갈조류 특유의 폴리페놀인 플로로탄닌이 다수 포함되어 있으므로, 상기 에틸아세테이트 분획의 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합 치환 효과 및 수면 유도 효과가 플로로탄닌에 의한 것일 가능성을 확인하기 위하여 상기 각각의 감태 추출물 분획의 총페놀함량을 분석하고, 총페놀함량과 GABAA-벤조다이아제핀 수용체 결합 활성 및 수면 유도 효과와의 상관관계를 분석하였다.Ethyl acetate fraction of Ecklonia cava extract contains a large number of phlorotannins, which are polyphenols unique to brown algae, and thus the effect of the ethyl acetate fraction on the substitution of GABA A -benzodiazepine ligand [ 3 H] flumazenil and sleep In order to confirm the possibility that the induction effect is caused by phlorotannin, the total phenol content of each Ecklonia cava extract fraction was analyzed and the correlation between total phenol content and GABA A -benzodiazepine receptor binding activity and sleep induction effect was investigated. Analyzed.
[표 6]은 감태 추출물 분획의 총페놀함량을 나타낸 것으로, 에틸아세테이트 분획에서 총페놀함량(TPC)가 가장 높고, 물 분획에서 가장 낮은 것을 확인하였다. 감태 에탄올 추출물(ECK-E)의 총페놀함량은 138.5 mgPEG/g으로 에틸아세테이트 분획 및 n-부탄올 분획이 감태 에탄올 추출물에 비해 총페놀함량이 높았다.[Table 6] shows the total phenolic content of the Ecklonia cava extract fraction, the highest total phenolic content (TPC) in the ethyl acetate fraction, the lowest in the water fraction. The total phenolic content of Ecklonia cava ethanol extract (ECK-E) was 138.5 mgPEG / g, which was higher in ethyl acetate fraction and n-butanol fraction than Ecklonia cava ethanol extract.
상기 각 감태 추출물 분획의 총페놀함량(TPC)와 [3H] 플루마제닐(flumazenil)의 결합에 대한 IC50의 상관관계를 도 5a에 나타내었고, 각 감태 추출물 분획의 총페놀함량(TPC)와 수면시간의 상관관계를 도 5b에 나타내었다.The correlation of IC 50 for the binding of the total phenolic content (TPC) and [ 3 H] flumazenil of each Ecklonia cava extract fraction is shown in Figure 5a, the total phenolic content (TPC) of each Ecklonia cava extract fraction The relationship between and sleep time is shown in Figure 5b.
상기 도 5a 및 도 5b에 따르면 각 추출물 분획의 결합 활성과 수면 유도 효과는 총페놀함량에 비례함을 알 수 있었다. 결합 활성의 비선형회기 분석에 따른 계수(R2)는 0.9544이었고, 수면시간과 총페놀함량 사이에도 100 mg/kg 농도에서는 R2가 0.8396, 200 mg/kg 농도에서는 R2가 0.9051로서 상관관계가 높음을 알 수 있었다.
5A and 5B, the binding activity and the sleep inducing effect of each extract fraction were found to be proportional to the total phenolic content. Was a factor (R 2) is 0.9544 in accordance with the non-linear regression analysis of the binding activity, even between sleep time and total
또한 상기 감태 추출물 분획과 감태 에탄올 추출물에 포함된 플로로탄닌 화합물의 종류 및 함량(mg/g)을 분석하여 [표 7]에 나타내었다.In addition, the type and content (mg / g) of the phlorotannin compound contained in the Ecklonia cava extract fraction and Ecklonia cava ethanol extract were analyzed and shown in [Table 7].
상기 각 감태 추출물 분획 및 감태 에탄올 추출물에서 검출된 플로로탄닌 화합물은 플로로글루시놀, 엑콜, 엑스톨로놀, 트리플로레톨-A 및 디엑콜이었다. 각 플로로탄닌 화합물들의 구조는 도 6에 나타내었다. The phlorotannin compounds detected in each of the Ecklonia cava extract fractions and Ecklonia cava ethanol extracts were phloroglucinol, ecchol, xtolonol, triloretol-A and dieckol. The structure of each phlorotannin compound is shown in FIG. 6.
결합 활성과 수면 유도 효과가 높았던 에틸아세테이트 분획과 n-부탄올 분획에서 총 플로로탄닌 화합물의 함량이 367.42 mg/g 및 177.11 mg/g으로 n-헥산 분획 및 물 분획에 비하여 현저히 높았다.In the ethyl acetate fraction and n-butanol fraction, which had high binding activity and sleep induction effect, the total phlorotannin compounds were 367.42 mg / g and 177.11 mg / g, which were significantly higher than the n-hexane and water fractions.
또한 각각의 분획에서 총 플로로탄닌 화합물의 함량과 수면시간의 상관관계는 100 mg/kg 농도에서는 R2가 0.9682, 200 mg/kg 농도에서는 R2가 0.9434로서 매우 높다는 것을 확인할 수 있었다.
In addition, it was confirmed that the correlation of the content and the sleeping time of the tannin compounds to the total flow in each of the fractions in the 100 mg / kg concentration R 2 is 0.9682, 200 mg / kg concentration in the R 2 is very high as 0.9434.
실험예Experimental Example 6: 6: 플로로탄닌Phlorotannin 화합물의 Compound GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 Receptor binding activity
상기 각 감태 추출물 분획에 존재하는 플로로탄닌 화합물들의 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐(flumazenil)의 결합 치환 효과를 나타내는 IC50값과 결합 친화도(Ki)를 계산하여 [표 8]에 나타내었다. 결합 친화도(Ki)는 아래 식(3)과 같이 계산하였다.IC 50 value and binding affinity (K i ) indicating the binding substitution effect of GABA A -benzodiazepine ligand [ 3 H] flumazenil of the phlorotannin compounds in each Ecklonia cava extract fraction were calculated. Table 8 shows. Binding affinity (K i ) was calculated as in the following equation (3).
식(3)Formula (3)
Ki=(IC50)/(1+([L]/Kd))K i = (IC 50 ) / (1 + ([ L ] / K d ))
([L]: the concentration of the radio-ligand ([3H]-flumazenil) used, Kd: the competitor-ligand dissociation equilibrium constant for [3H]-flumazenil. The Kd value is 1.6 nM)([ L ]: the concentration of the radio-ligand ([ 3 H] -flumazenil) used, K d : the competitor-ligand dissociation equilibrium constant for [ 3 H] -flumazenil.The K d value is 1.6 nM)
플로로탄닌 화합물의 결합 친화도의 상대적 강도를 확인하기 위하여 양성 대조구으로 디아제팜(DZP)를 사용하였다. 엑콜과 엑스톨로놀은 트리플로레톨-A나 디엑콜에 비해 결합 친화도가 높았고, 플로로탄닌의 기본 골격이 되는 플로로글루시놀의 경우 1000 μM에서 42.25%의 저해를 나타내어 결합 친화도가 다른 플로로탄닌에 비해 높지 않았다.
Diazepam (DZP) was used as a positive control to confirm the relative strength of the binding affinity of the phlorotannin compound. Eccol and Xtolonol showed higher binding affinity than Triloretol-A or Diexol. In the case of phloroglucinol, which is the basic skeleton of phlorotannin, 42.25% inhibition was observed at 1000 μM. It was not higher than other phlorotannins.
실험예Experimental Example 7: 갈조류 추출물의 7: of brown algae extract 총페놀함량과Total phenolic content and GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 및 수면 유도 효과의 상관관계 Correlation of Receptor Binding Activity and Sleep Induction Effect
상기 [표 3]의 갈조류 중에서 GABAA-벤조다이아제핀 수용체 결합 활성이 다른 참가죽그물바탕말(Dictyota coriacea), 가시뼈대그물말(Dictyopteris prolifera Okamura), 감태(Ecklonia cava Kjellman)를 선별하고, GABAA-벤조다이아제핀 수용체 결합 활성이 높았던 감태와 동일속의 검둥감태(Ecklonia kurome)를 추가하여 각각의 메탄올 추출물의 총페놀함량을 측정하고, 총페놀함량과 GABAA-벤조다이아제핀 수용체 결합 활성 및 수면 유도 효과의 상관관계를 확인하였다.Participants with different GABA A -benzodiazepine receptor binding activity in brown alga of [Table 3] ( Dictyota coriacea ), spiny skeletal net ( Dictyopteris) prolifera Okamura) and Ecklonia cava Kjellman were screened, and the total phenolic content of each methanol extract was measured by adding Ecklonia kurome of the same rate as Ecklonia kurome , which had high GABA A -benzodiazepine receptor binding activity. The correlation between phenol content and GABA A -benzodiazepine receptor binding activity and sleep induction effect was confirmed.
[표 9]는 4종의 갈조류 메탄올 추출물의 총페놀함량을 측정한 것으로,감태 추출물 분획의 총페놀함량을 나타낸 것으로 검둥감태는 감태와 총페놀함량이 유사하였고, 가시뼈대그물말, 참가죽그물바탕말 순서로 함량이 낮아졌다. Table 9 shows the total phenolic content of the four kinds of brown algae methanol extracts, and shows the total phenolic content of the Ecklonia cava extract. The contents were lowered in the order of the background words.
Alariaceae
상기 검둥감태 메탄올 추출물, 감태 메탄올 추출물, 가시뼈대그물말 메탄올 추출물 및 참가죽그물바탕말 메탄올 추출물의 총페놀함량(TPC)와 10 mg/mL농도에서의 [3H] 플루마제닐(flumazenil)의 결합 활성의 상관관계를 도 7a에 나타내었고, 각 갈조류 메탄올 추출물의 총페놀함량(TPC)와 수면시간의 상관관계를 도 7b에 나타내었다.The total phenolic content (TPC) and the concentration of [ 3 H] flumazenil at 10 mg / mL concentrations of the methanol extract of E. coli, E. coli methanol, extract of spiny skeletal net methanol and extract of methanol The correlation of binding activity is shown in FIG. 7A, and the correlation between total phenolic content (TPC) and sleep time of each brown algae methanol extract is shown in FIG. 7B.
상기 도 5a 및 도 5b에서 감태 추출물 분획의 총페놀함량에 따라 결합 활성과 수면 유도 효과가 총페놀함량에 비례하는 것과 동일하게, 도 7a 및 도 7b에서도 각각의 갈조류의 총페놀함량과 결합 활성이나 수면 유도 효과가 비례관계에 있음을 확인할 수 있었다.
5A and 5B, the binding activity and the sleep induction effect according to the total phenolic content of the Ecklonia cava extract fractions are proportional to the total phenolic content. In FIG. 7A and FIG. 7B, the total phenolic content and the binding activity of the brown algae Sleep induction effect was found to be in proportion.
실험예Experimental Example 8: 8: 검둥Nigger 감태Ecklonia 추출물의 Extract GABAGABA AA -- 벤조다이아제핀Benzodiazepines 수용체 결합 활성 및 수면 유도 효과 Receptor binding activity and sleep induction effect
감태 추출물과 마찬가지로 같은 속에 속하는 검둥감태 역시 GABAA-벤조다이아제핀 수용체 결합 활성 및 수면 유도 효과를 나타내는지를 확인하였다.Like Ecklonia cava extracts, black gestatione belonging to the same genus also showed GABA A -benzodiazepine receptor binding activity and sleep induction effect.
도 8a는 검둥감태 메탄올 추출물의 방사성 표지된 GABAA-벤조다이아제핀 리간드 [3H] 플루마제닐의 결합억제 효과를 나타낸 그래프로서, 검둥감태 메탄올 추출물(EKO-M)의 경우 GABAA-벤조다이아제핀 수용체에 대한 친화력이 농도의존적으로 높아졌으며, IC50 값은 0.626 mg/mL이었다.FIG. 8A is a graph showing the binding inhibitory effect of radiolabeled GABA A -benzodiazepine ligand [ 3 H] flumagenyl in the black ethanol methanol extract, and GABA A -benzodiaza for the black amber methanol extract (EKO-M) Affinity for the zepin receptors was increased concentration-dependent, with an IC 50 value of 0.626 mg / mL.
또한 도 8b와 도 8c는 감태 메탄올 추출물(ECK-M)과 검둥감태 메탄올 추출물(EKO-M)이 hypnotic 농도의 펜토바비탈(45 mg/kg, 복강주사)에서의 입면시간(sleep latency)과 수면시간(sleep duration)을 측정하여 나타낸 것으로, 검둥감태 메탄올 추출물은 수면시간에 있어서 유의적인 효과가 있음을 확인할 수 있다.
8B and 8C also show that the Ecklonia cava extract (ECK-M) and the Ecklonia cava extract (EKO-M) were shown to sleep latency at pentobarbital (45 mg / kg, intraperitoneal injection) of hypnotic concentration. As a result of measuring the sleep duration, it can be seen that the black-brown Ecklonia cava extract has a significant effect on the sleep time.
실험예Experimental Example 9: 갈조류 추출물과 9: brown algae extract 다이아제팜Diazepam 병용경구투여에In combination oral administration 대한 About 펜토바비탈Pentobarbital 수면유도 시너지 효과 Sleep Induction Synergy
감태 에탄올 및 물 추출물과 다이아제팜의 병용경구투여에 대한 수면유도 시너지 효과를 알아보기 위하여 펜토바비탈의 수면유도효과에 영향을 주지 않을 만큼의 낮은 농도로 ECK-E는 100 mg/kg, ECK-W는 100 mg/kg, DZP는 0.5 mg/kg을 펜토바비탈 투여(hypnotic dosage 45 mg/kg) 45분 전에 경구 투여하여 입면시간(sleep latency)과 수면시간(sleep duration)을 측정한 그 결과를 도 9a 및 도 9b에 나타내었다. 각 그래프는 평균값(means±SEM, n=10)을 나타낸다.ECK-E was 100 mg / kg, ECK- W and 100 mg / kg and DZP 0.5 mg / kg were orally administered 45 minutes prior to pentobarbital (hypnotic dosage 45 mg / kg) to measure sleep latency and sleep duration. Are shown in FIGS. 9A and 9B. Each graph shows the mean value (means ± SEM, n = 10).
*은 대조구와 비교하여 p<0.05에서, ***은 대조구와 비교하여 p<0.001에서 유의적 차이가 있음(Dunnet's test)을 의미하고, #은 p<0.05에서, ##은 p<0.01에서 병용 투여한 그룹과 단일 투여한 그룹 사이의 유의적인 차이가 있다는 것을 의미한다.(Unpaired Student's t-test) 그리고, NS는 유의적인 차이가 없다는 것을 의미한다.* Indicates a significant difference at p <0.05 compared to the control, *** at p <0.001 compared to the control (Dunnet's test), # at p <0.05, ## at p <0.01 Unpaired Student's t-test means that there is a significant difference between the group administered with the single dose and the group given with the single dose.
도 9a 및 도 9b에서 보는 바와 같이, 본 발명에 따른 감태 에탄올 및 물 추출물을 DZP와 병용투여했을 때 수면시간에 있어서 유의적인 효과가 있음을 확인할 수 있다.
As shown in Figure 9a and Figure 9b, it can be seen that when the Ecklonia cava ethanol and water extract according to the present invention in combination with DZP has a significant effect on sleep time.
실험예Experimental Example 10: 10: 플루마제닐이Flumazenil 갈조류 추출물의 수면 유도 효과에 미치는 영향 Effects of Brown Algae Extracts on Sleep Induction Effects
GABAA-벤조다이아제핀 길항제인 플루마제닐(FLU)이 실험예 2의 방법으로 제조된 감태 에탄올 추출물(ECK-E), 감태 물 추출물(ECK-W) 및 실험예 4의 방법으로 제조된 에틸아세테이트 분획(EtOAc)의 수면유도 효과에 미치는 영향에 대해서 알아보기 위하여 ECK-E는 1000 mg/kg, ECK-W는 1000 mg/kg, EtOAc는 200 mg/kg, DZP는 0.5 mg/kg을 펜토바비탈 투여(hypnotic dosage 45 mg/kg) 45분 전에 경구투여하였고, FLU는 8 mg/kg을 ECK-E, ECK-W, EtOAc, DZP 경구 투여 10분전에 미리 복강 주사하여 입면시간(sleep latency)과 수면시간(sleep duration)을 측정하였다. 그 결과를 하기 도 10a 및 도 10b에 나타내었다. 각 그래프는 평균값(means±SEM, n=10)을 나타낸다.Flumazenyl (FLU), a GABA A -benzodiazepine antagonist, was prepared by the method of
*은 대조구와 비교하여 p<0.05에서, **은 p<0.01에서, ***은 p<0.001에서 유의적 차이가 있음(Dunnet's test)을 의미하고,* Means significant difference at p <0.05, ** at p <0.01, *** at p <0.001 compared to the control (Dunnet's test),
#은 p<0.05에서, ##은 p<0.01에서, ###은 p<0.001에서, 플루마제닐을 처리한 그룹과 처리하지 않는 그룹 사이의 유의적인 차이가 있다는 것을 의미한다.(Unpaired Student's t-test) 그리고 NS는 유의적인 차이가 없다는 것을 의미한다.# At p <0.05, ## at p <0.01, ### at p <0.001, meaning there is a significant difference between flumagenyl and untreated groups (Unpaired Student's t-test) and NS mean no significant difference.
도 10a 및 도 10b에서 보는 바와 같이, 대표적인 수면제 중의 하나인 다이아제팜은 잘 알려진 GABAA-벤조다이아제핀의 활성 효능제로서, 다이아제팜의 수면효과는 GABAA-벤조다이아제핀 길항제인 플루마제닐에 의해 억제되었다.As shown in Figs. 10a and 10b, a typical sleeping GABA A, one of the diamonds is well known the farm of - as an active agonist of benzodiazepine, sleep effect of diazepam is GABA A - a benzodiazepine antagonist flu horseshoe carbonyl Inhibited by
또한, 본 발명에 따른 감태 물 추출물(ECK-W), 에탄올 추출물(ECK-E) 및 에틸아세테이트 분획(EtOAc)도 수면제 다이아제팜과 마찬가지로 GABAA-벤조다이아제핀 길항제인 플루마제닐에 의해 그 효과가 억제되었다.In addition, the effects of the Ecklonia cava extract (ECK-W), ethanol extract (ECK-E) and ethyl acetate fraction (EtOAc) according to the present invention by flumazenyl, which is a GABA A -benzodiazepine antagonist, as well as hypnotic diazepam Was suppressed.
상기 결과에서, 갈조류 추출물은 기존의 수면제인 디아제팜과 마찬가지로 GABAA-벤조다이아제핀 수용체에 친화력을 가지며 효능제의 역할을 함을 확인할 수 있다. 그리고, 마우스를 이용한 동물실험에서도 수면유도 효과를 나타내었으며, 디아제팜과 마찬가지로 GABAA-벤조다이아제핀 길항제에 의해 그 효과가 감소함을 알 수 있다.
In the above results, brown algae extract can be confirmed that has affinity to the GABA A -benzodiazepine receptor and acts as an agonist like diazepam, a conventional sleeping agent. In addition, the animal experiment using the mouse showed a sleep-inducing effect, it can be seen that the effect is reduced by GABA A -benzodiazepine antagonist like diazepam.
실험예Experimental Example 11: 갈조류 추출물이 쥐의 수면구조에 미치는 영향 11: Effects of Brown Algae Extracts on Sleep Structure in Rats
SD rat(200-250 g)를 1주일간 적응시킨 후 뇌파(Electroencephalogram, EEG) 및 근전도도(Electromyogram, EMG) 측정을 위해 전극 삽입 수술을 실시하였다. Rat를 펜토바비탈(50 mg/kg, i.p.)로 마취시키고 뇌정위기(stereotaxic instrument)에 두부를 고정시켰다. 두부 피하 결합조직을 절개한 후 EEG 및 EMG 측정을 위하여 stainless-steel screw 와 silver electrode line을 삽입하였다. 이후 치과용 dental cement로 고정시키고 봉합하였다. 수술부위의 소독 및 항생제 투여를 3일 동안 실시하여 수술로 인한 염증을 예방하였으며, 7일간 회복기간을 두었다. 측정환경에 적응시키기 위하여 측정 4일 전부터 control 실험군에 사용하는 0.5% CMC-saline 용액을 경구투여(p.o.)한 후 recording 장치를 연결하여 본 실험과정에 순응하도록 유도하였다.SD rats (200-250 g) were acclimated for 1 week and then subjected to electrode insertion for electroencephalogram (EGG) and electromyogram (EMG) measurements. Rats were anesthetized with pentobarbital (50 mg / kg, i.p.) and the head was fixed in a stereotaxic instrument. After cutting the subcutaneous connective tissue, a stainless-steel screw and a silver electrode line were inserted for EEG and EMG measurements. After fixing with dental dental cement and sutured. Disinfection of the surgical site and administration of antibiotics were performed for 3 days to prevent inflammation due to surgery and a recovery period of 7 days was allowed. In order to adapt to the measurement environment, 0.5% CMC-saline solution used in the control group was orally administered (p.o.) from 4 days before the measurement, and the recording device was connected to induce compliance with the experimental procedure.
EEG 및 EMG는 PAL-8200 series(Pinnacle Technology Inc, Oregon, USA)를 이용하여 10:00부터 16:00까지 6시간 동안 측정하였다. EEG 및 EMG의 sampling rate는 200 Hz로 설정하고(epoch time : 10초), EEG는 0.1-25 Hz, EMG는 10-100 Hz의 필터 영역을 설정하여 데이터를 기록하였다.EEG and EMG were measured for 6 hours from 10:00 to 16:00 using the PAL-8200 series (Pinnacle Technology Inc, Oregon, USA). The sampling rate of EEG and EMG was set to 200 Hz (epoch time: 10 seconds), and the data was recorded by setting the filter area of 0.1-25 Hz for EEG and 10-100 Hz for EMG.
수면구조 분석은 fast Fourier transform(FFT) 알고리즘에 의해 수행되었으며, SleepSign 프로그램(Ver. 3.0, Kissei Comtec, Nagono, Japan)을 이용하였다.Sleep structure analysis was performed by a fast Fourier transform (FFT) algorithm, using the SleepSign program (Ver. 3.0, Kissei Comtec, Nagono, Japan).
분석결과는 wake, REM sleep(rapid eye movement, theta band: 6-10 Hz), NREM sleep(non-rapid eye movement, delta band: 0.65-4 Hz)으로 구분하여 나타내었다. Sleep latency(입면시간)는 10초 epoch 단위의 NREM 수면이 12번 이상 연속적으로 나타나는데 걸리는 시간으로 설정하였다.The analysis results were divided into wake, REM sleep (rapid eye movement, theta band: 6-10 Hz), and NREM sleep (non-rapid eye movement, delta band: 0.65-4 Hz). Sleep latency was set as the time taken for NREM sleep in 10-second epoch units to continuously appear more than 12 times.
도 11a에서 보는 바와 같이, 본 발명에 따른 감태 에탄올 추출물 500 mg/kg을 경구투여 하여 수면 구조를 분석한 결과 입면시간(sleep latency)이 40.7분에서 31.8분으로 약 9분정도 유의적으로 단축 되었다. As shown in Figure 11a, the sleep structure was analyzed by oral administration of 500 mg / kg Ecklonia cava ethanol extract according to the present invention significantly reduced the sleep latency (about 9 minutes from 40.7 minutes to 31.8 minutes) .
또한 도 11b의 수면 구조에서 NREM 수면시간이 42.5%에서 54.6%로 약 12% 증가하면서 수면증진에 효과가 있었다.
In addition, in the sleep structure of FIG. 11B, the NREM sleep time was increased by about 12% from 42.5% to 54.6%, thereby improving sleep.
실험예Experimental Example 12: 12: 플로로탄닌Phlorotannin 농축 분획의 항경련 효과 Anticonvulsant Effect of Concentrated Fractions
실험예 1에서 90% 이상의 GABAA-벤조다이아제핀 수용체 결합 활성이 가장 뛰어났던 감태(Ecklonia cava Kjellman, ECK)에서 효소를 이용한 효소 추출물을 얻고, 그 효소 추출물에서 플로로탄닌 농축 분획을 얻어, 효소추출물(ECEE) 및 플로로탄닌 농축 분획(PTRF)의 총페놀함량 및 항경련 효과를 확인하였다.
In Experimental Example 1, an enzyme extract using an enzyme was obtained from Ecklonia cava Kjellman (ECK), which had the highest GABA A -benzodiazepine receptor binding activity of 90% or more, and a concentrated fraction of phlorotannin was obtained from the enzyme extract. The total phenolic content and anticonvulsant effect of the extract (ECEE) and phlorotannin concentrated fraction (PTRF) were confirmed.
1) 효소추출물(ECEE) 및 플로로탄닌 농축 분획(PTRF)의 제조1) Preparation of Enzyme Extract (ECEE) and Florotannin Concentrated Fraction (PTRF)
1 kg의 동결건조 감태 분말과 증류수 10 L를 혼합한 후, 셀룰라아제(Celluclast , Novo Nordisk, Bagsvaerd, Denmark) 10 mL를 첨가하고 50 ℃에서 24 시간 반응시키고, 100 ℃에서 10분 동안 효소를 불활성화시켰다. 3000 x g에서 20분동안 원심분리하여 가수분해되지 않은 잔사를 제거하여 얻은 여액을 얻고, 이를 농축한 후 동결건조하여 효소추출물(ECEE)을 얻었다.After mixing 1 kg of lyophilized Ecklonia cava powder and 10 L of distilled water, 10 mL of cellulase (Celluclast, Novo Nordisk, Bagsvaerd, Denmark) was added and reacted at 50 ° C. for 24 hours, and the enzyme was inactivated at 100 ° C. for 10 minutes. I was. Centrifugation at 3000 x g for 20 minutes yielded a filtrate obtained by removing the non-hydrolyzed residue, which was concentrated and lyophilized to obtain an enzyme extract (ECEE).
상기 효소추출물을 동결건조하기 전의 농축물을 70% 에탄올과 혼합한 후 24시간 교반하고, 교반한 혼합물을 3500 x g에서 20분 동안 원심분리한 후 와트만 여과지 No.1으로 잔사를 제거하여 여액을 얻고, 이를 농축한 후 동결건조하여 플로로탄닌 농축 분획(PTRF)를 얻었다.
The concentrate before freeze-drying the enzyme extract was mixed with 70% ethanol and stirred for 24 hours, the stirred mixture was centrifuged at 3500 xg for 20 minutes, and the residue was removed with Whatman filter paper No. 1 to obtain the filtrate. It was concentrated, and then lyophilized to obtain a phlorotannin concentrated fraction (PTRF).
2) 피크로톡신(picrotoxin) 유도 발작(seizure) 시험2) Picrotoxin-induced seizure test
실험동물은 실험예 3과 같이 준비하고, 쥐에 대조군(CON, 0.5%, CMC-saline 10 mL/kg), ECEE 및 DZP를 경구 투여하였고, 경구 투여 45분 후, 7 mg/kg의 피크로톡신을 정맥주사하여 발작을 유도하였다. 피크로톡신을 투여한 쥐는 바로 개별 케이지에 넣고 90분 동안 관찰하여, 경련의 횟수와 피크로톡신 투여 후부터 발작의 발현까지의 발작 잠재 기간을 기록하였다. 90분동안 발작이 발현되지 않는 경우 발작 잠재 기간은 0분으로 기록하였다.
Experimental animals were prepared as in Experimental Example 3, and mice were orally administered with a control group (CON, 0.5%, CMC-
3) 실험결과3) Experiment result
[표 10]은 효소추출물(ECEE) 및 플로로탄닌 농축 분획(PTRF)의 총페놀함량을 나타낸 것이고, Residue는 RTRF를 제외한 ECEE부분을 의미한다. 플로로탄닌 농축 분획에는 효소추출물에 비하여 6 배 이상 많은 총페놀함량을 나타내었다.Table 10 shows the total phenolic content of the enzyme extract (ECEE) and phlorotannin enriched fraction (PTRF), and Residue means the ECEE portion excluding RTRF. In the concentrated fraction of phlorotannin, the total phenol content was 6 times higher than that of the enzyme extract.
효소추출물(ECEE)의 항경련 효과는 [표 11]에 나타내었고, 플로로탄닌 농축 분획(PTRF)의 항경련 효과는 [표 12]에 나타내었다. The anticonvulsant effect of the enzyme extract (ECEE) is shown in [Table 11], and the anticonvulsant effect of the phlorotannin concentrated fraction (PTRF) is shown in [Table 12].
(mg/kg)Dose
(mg / kg)
Convulsed
/totalNo. mice
Convulsed
/ total
(%)Final mortality
(%)
ECEE
**은 대조구와 비교하여 p<0.01에서 유의적 차이가 있다.(Dunnet's test)** has a significant difference at p <0.01 compared to the control (Dunnet's test)
[표 11]에서 대조군(CON)의 최종 사망율은 80% 이었고, 양성 대조군으로 사용된 디아제팜(DZP)은 발작 잠재기간이 현저히 지연되었다. 효소추출물(ECEE)의 경우에도 용량의존적으로 최종 사망율을 낮추고 발작 잠재기간을 지연시키는 효과를 나타내었고, 특히 1000 mg/kg 용량에서 유의미한 발작 잠재기간의 증가가 나타났다.In Table 11, the final mortality rate of the control group (CON) was 80%, and the diazepam (DZP) used as a positive control group significantly delayed the seizure duration. In the case of enzyme extract (ECEE), the dose-dependent effect also lowered the final mortality rate and delayed seizure duration, especially at 1000 mg / kg dose.
(mg/kg)Dose
(mg / kg)
Convulsed
/totalNo. mice
Convulsed
/ total
(%)Final mortality
(%)
PTRF
[표 12]에서 플로로탄닌 농축 분획(PTRF)은 효소추출물(ECEE)와 마찬가지로 최종 사망율을 낮추고 발작 잠재기간을 지연시키는 효과를 나타내었고, 특히 ECEE에 비해서 1/4 내지 1/10 정도의 용량으로도 ECEE와 동등한 수준의 항경련 효과를 나타내었다.
In Table 12, the phlorotannin-enriched fraction (PTRF), like the enzyme extract (ECEE), had the effect of lowering the final mortality rate and delaying seizure duration, in particular 1/4 to 1/10 the dose compared to ECEE. In addition, it showed the same level of anticonvulsion effect as ECEE.
실험예Experimental Example 13: 통계분석 13: Statistical Analysis
50% 억제농도(IC50) 값은 Prism 5.0 프로그램을 이용하여 one-site competition binding model을 적용시켜 산출하였다. 모든 실험의 결과는 평균과 표준오차(means±SEM)으로 나타내었고, 각 군과 대조구의 통계적인 비교는 Dunnet's test에 의한 One-way-analysis of variance(ANOVA)를 통해서 평가하였다. 유의 수준의 표시는 p<0.05(*), P<0.01(**), p<0.001(***) 수준에서 유의성을 보이는 결과를 별표로 표시하였다. 두 그룹간의 데이터 비교는 Unpaired Student's t-test에 의해서 분석하였고, p<0.05(#), p<0.01(##), p<0.001(###) 수준에서 두 그룹간의에 통계적으로 유의적 차이가 있음을 표시하였다. 통계분석 프로그램은 Prism 5.0 소프트웨어를 사용하여 수행하였다.
The 50% inhibitory concentration (IC 50 ) was calculated by applying the one-site competition binding model using the Prism 5.0 program. The results of all experiments were expressed as mean and standard error (means ± SEM), and statistical comparison of each group and control was evaluated by One-way-analysis of variance (ANOVA) by Dunnet's test. The significance level was marked with an asterisk indicating the results showing significance at p <0.05 (*), P <0.01 (**), and p <0.001 (***) levels. Data comparison between the two groups was analyzed by Unpaired Student's t-test, and statistically significant differences between the two groups at the levels of p <0.05 (#), p <0.01 (##) and p <0.001 (###) Indicated that there is. Statistical analysis program was performed using Prism 5.0 software.
하기에 본 발명의 추출물을 함유하는 조성물의 제제예를 설명하나, 본 발명은 이를 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, the formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to limit the present invention but only to explain in detail.
제제예Formulation example 1. One. 산제의Sanje 제조 Produce
실험예 1에서 얻은 감태 에탄올 추출물 분말 20 mg20 mg of Ecklonia cava ethanol extract powder obtained in Experimental Example 1
유당 100 mg
탈크 10 mg
상기의 성분들을 혼합하고 기밀포에 충진하여 산제를 제조한다.
The above components are mixed and filled in airtight bags to prepare powders.
제제예Formulation example 2. 정제의 제조 2. Preparation of tablets
실험예 1에서 얻은 감태 에탄올 추출물 분말 10 mg10 mg Ecklonia cava ethanol extract powder obtained in Experimental Example 1
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.
After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.
제제예Formulation example 3. 캅셀제의 제조 3. Preparation of capsules
실험예 1에서 얻은 감태 에탄올 추출물 분말 10 mg10 mg Ecklonia cava ethanol extract powder obtained in Experimental Example 1
결정성 셀룰로오스 3 mg
락토오스 14.8 mgLactose 14.8 mg
마그네슘 스테아레이트 0.2 mgMagnesium stearate 0.2 mg
통상의 캡슐제 제조방법에 따라 상기의 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조한다.
The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.
제제예Formulation example 4. 주사제의 제조 4. Preparation of injections
실험예 1에서 얻은 감태 에탄올 추출물 분말 10 mg10 mg Ecklonia cava ethanol extract powder obtained in Experimental Example 1
만니톨 180 mg180 mg mannitol
주사용 멸균 증류수 2974 mgSterile sterilized water for injection 2974 mg
Na2HPO4,12H2O 26 mgNa 2 HPO 4, 12H 2 O 26 mg
통상의 주사제의 제조방법에 따라 1 앰플 당 상기의 성분 함량으로 제조한다.
According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule.
제제예Formulation example 5. 5. 액제의Liquid 제조 Produce
실험예 1에서 얻은 감태 에탄올 추출물 분말 20 mg20 mg of Ecklonia cava ethanol extract powder obtained in Experimental Example 1
이성화당 10 g10 g per isomer
만니톨 5 g5 g mannitol
정제수 적량Purified water quantity
통상의 액제의 제조방법에 따라 정제수에 각각의 성분을 가하여 용해시키고 레몬향을 적량 가한 다음 상기의 성분을 혼합한 다음 정제수를 가하여 전체를 정제수를 가하여 전체 100로 조절한 후 갈색병에 충진하여 멸균시켜 액제를 제조한다.
Each component was added to purified water in accordance with the conventional liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water and adjusted to 100 as a whole. To prepare a liquid agent.
제제예Formulation example 6. 건강기능식품의 제조 6. Manufacture of health functional foods
실험예 1에서 얻은 감태 에탄올 추출물 분말 1,000 mg1,000 mg Ecklonia cava ethanol extract powder obtained in Experimental Example 1
비타민 혼합물 적량Vitamin mixture quantity
비타민 A 아세테이트 70 ㎍70 [mu] g of vitamin A acetate
비타민 E 1.0 mgVitamin E 1.0 mg
비타민 B1 0.13 mgVitamin B1 0.13 mg
비타민 B2 0.15 mg0.15 mg of vitamin B2
비타민 B6 0.5 mgVitamin B6 0.5 mg
비타민 B12 0.2 ㎍0.2 [mu] g vitamin B12
비타민 C 10 mg
비오틴 10 ㎍Biotin 10 μg
니코틴산아미드 1.7 mgNicotinic acid amide 1.7 mg
엽산 50 ㎍50 ㎍ of folic acid
판토텐산 칼슘 0.5 mgCalcium pantothenate 0.5 mg
무기질 혼합물 적량Mineral mixture quantity
황산제1철 1.75 mg1.75 mg of ferrous sulfate
산화아연 0.82 mg0.82 mg of zinc oxide
탄산마그네슘 25.3 mgMagnesium carbonate 25.3 mg
제1인산칼륨 15 mgPotassium monophosphate 15 mg
제2인산칼슘 55 mgSecondary calcium phosphate 55 mg
구연산칼륨 90 mgPotassium citrate 90 mg
탄산칼슘 100 mg
염화마그네슘 24.8 mgMagnesium chloride 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 건강기능식품에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강기능식품 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강기능식품 조성물 제조에 사용할 수 있다.
Although the composition ratio of the above-mentioned vitamin and mineral mixtures is a composition that is relatively suitable for the health functional food, the composition is mixed in a preferred embodiment, but the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, the granules may be prepared and used for preparing the nutraceutical composition according to a conventional method.
제제예Formulation example 7. 기능성 음료의 제조 7. Preparation of Functional Beverages
실험예 1에서 얻은 감태 에탄올 추출물 분말 1,000 mg1,000 mg Ecklonia cava ethanol extract powder obtained in Experimental Example 1
구연산 1,000 mgCitric acid 1,000 mg
올리고당 100 g100 g of oligosaccharide
매실농축액 2 gPlum concentrate 2 g
타우린 1 gTaurine 1 g
정제수를 가하여 전체 900 mLPurified water was added to the flask to obtain a total of 900 mL
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1 시간 동안 85 ℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2 L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 기능성 음료 조성물 제조에 사용한다. The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 수요계층, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.
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KR20180076607A (en) * | 2016-12-28 | 2018-07-06 | 인천대학교 산학협력단 | Cosmetic composition comprising ishige okamurae extract with sleeping induction function, and cosmetics using the same |
KR102646051B1 (en) * | 2022-09-22 | 2024-03-13 | 국립부경대학교 산학협력단 | A composition for the prevention, improvement or treatment of sleep disorders or insomnia containing Gelidium amansii extract |
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KR101474749B1 (en) * | 2013-03-27 | 2014-12-23 | 부경대학교 산학협력단 | Composition for anxiolitic, anti-convulsant, anti-depressant or sleep-improving effect comprising shellfish extract as an effective component |
KR101804613B1 (en) * | 2014-06-05 | 2017-12-04 | 재단법인 제주테크노파크 | Anti-stress Composition |
KR101823673B1 (en) | 2017-01-18 | 2018-01-30 | 배대열 | Sleep inducing composition with nipa fruticans wurmb |
KR101899546B1 (en) | 2017-05-11 | 2018-09-17 | 한국식품연구원 | Composition for improving, preventing or treating sleep disorders, or composition for inhibitiong resistance or reducing side effects of GABA A receptor agonist, comprising phloroglucinol as an effective ingredient |
KR102154052B1 (en) * | 2017-10-26 | 2020-09-10 | 주식회사 보타메디 | Compositions containing phlorotannin for cooling skin and product thereof |
KR102205207B1 (en) * | 2019-04-26 | 2021-01-20 | 주식회사 그린러쉬 농업회사법인 | Beverage composition containing hemp seed extract |
KR102456193B1 (en) | 2020-03-03 | 2022-10-21 | 정재연 | Sleep inducing tea containing l-theanine |
KR102588771B1 (en) | 2021-05-24 | 2023-10-13 | 한국생명공학연구원 | COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING |
KR102548951B1 (en) * | 2021-08-12 | 2023-06-29 | 주식회사 머스카 | Use of natural products to induce sleep and improve sleep quality |
KR102564539B1 (en) * | 2022-12-16 | 2023-08-09 | 부경대학교 산학협력단 | A composition for the prevention, improvement or treatment of sleep disorders or insomnia containing Codium fragile extract |
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JP3543175B2 (en) * | 2001-01-16 | 2004-07-14 | 北海道 | α-glucosidase inhibitor |
JP2006241039A (en) * | 2005-03-02 | 2006-09-14 | Nagase & Co Ltd | Urease inhibitor |
JP2007217339A (en) * | 2006-02-16 | 2007-08-30 | Kanehatsu Foods Co Ltd | Anti-allergic substance |
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KR20180076607A (en) * | 2016-12-28 | 2018-07-06 | 인천대학교 산학협력단 | Cosmetic composition comprising ishige okamurae extract with sleeping induction function, and cosmetics using the same |
KR102646051B1 (en) * | 2022-09-22 | 2024-03-13 | 국립부경대학교 산학협력단 | A composition for the prevention, improvement or treatment of sleep disorders or insomnia containing Gelidium amansii extract |
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JP6014731B2 (en) | 2016-10-25 |
KR101260697B1 (en) | 2013-05-10 |
KR20120079003A (en) | 2012-07-11 |
JP2014505683A (en) | 2014-03-06 |
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