KR20180076607A - Cosmetic composition comprising ishige okamurae extract with sleeping induction function, and cosmetics using the same - Google Patents

Abstract

The present invention relates to a functional composition for inducing sleep, which comprises an extract of Ishige okamurae as an active ingredient. More specifically, by firstly confirming that an extract of one of marine algae, Ishige okamurae, increases the expressions of melatonin and a melatonin receptor (MT1) and thus induces sleep, the present invention relates to the use of such an extract of Ishige okamurae as an active ingredient of a functional cosmetic composition, a food composition, or a pharmaceutical composition for inducing sleep.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a skin extract having a water-inducing function and a cosmetic composition using the same. 2. Description of the Related Art Cosmetic compositions,

The present invention is based on the idea that Ishige okamurae) relates to a sleep inducing functional composition comprising the extract as an active ingredient, and more particularly, to a hand one of the seaweed (Ishige okamurae extract of the present invention has an effect of inducing sleeping by increasing the expression of melatonin and melatonin receptor (MT1), and it has been confirmed that these extracts can be used as an effective ingredient of a functional cosmetic composition for food, a food composition or a pharmaceutical composition And the use thereof.

As society develops rapidly, there are increasing factors that can cause a lot of stress and anxiety, and the number of people suffering from sleep disorders is increasing. Furthermore, in the reality that diseases such as insomnia and nervous anxiety due to the aging of the population are increasing, it is urgently required to develop medicines or ancillary products with less side effects that can be treated and prevented.

Melatonin is known to be a major regulatory mechanism in the regulation of circadian rhythm including sleep induction. Specifically, increased melatonin is mediated by the melatonin receptors (MT1, MT2) on the cell walls, which regulate sleep induction in a way that signals sleep induction.

Therefore, in relation to the development of materials and medicines for controlling sleep, the contents of melatonin in the body and the melatonin receptors may act as important target molecules for controlling the circadian cycle including the induction of sleep.

Ishige Okamurae ) is a marine vegetable brown algae distributed in the southern coast of Korea and Jejudo coast. Some studies have been conducted to investigate its efficacy.

Korean Patent No. 10-0981184, for example, discloses that Lactobacillus root extract has an antioxidative activity and discloses a natural antioxidant using it as an active ingredient.

However, still it lost (Ishige up Studies on other diverse efficacies of okamurae have been limited , and there have been no studies that have shown that Lepidoptera induces sleep induction or sleep induction.

Korean Patent No. 10-0981184

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned conventional needs, The results showed that the induction of melatonin and the changes of melatonin receptor expression in the treatment of Lappa extract resulted in a significant increase in sleep Inducing activity, and reached the present invention.

That is, a technical object of the present invention is to provide a functional composition, particularly a cosmetic composition, comprising a Lactuca extract having a water-inducing function.

To achieve the above-mentioned technical problem, the present invention provides the L (Ishige The present invention provides a water- insoluble functional composition comprising an extract of Okamurae as an active ingredient.

The invention marine brown algae one kinds of L (Ishige of as an extract sleep induction effect first found that the presence in the okamurae), the cellular level (in vitro) and animal (in vivo) experiments melatonin (melatonin) hormones for 4 weeks L extract that play an important role in sleep induction The increase in melatonin receptor (MT1) expression was observed in the serum of the fed mice and when the extract was administered to brain cells (glioma cells), the expression of melatonin receptor 1 (MT1) Melatonin receptor (MT1) increased in the brain of one mouse compared to the control group.

In other words, the Lard extract used in the present invention is excellent in not only the increase of melatonin hormone directly related to the induction of sleep but also the increase of the melatonin receptor which is responsible for the intracellular signal transduction (sleep surface induction signal transduction) function by melatonin And it was found that it exhibited sleep inducing efficacy.

Specifically, the Lahmang extract used in the present invention contains more than 10% of DPHC (diphloroethohydroxycarmalol) component (see Table 1 below), and this component is considered to cause a sleep inducing effect.

[Table 1]

In the present invention, there is no particular limitation on the method for obtaining the Lactobacillus root extract, and the Lactobacillus root extract can be obtained by a conventional method in the field of extracting the active ingredient from natural materials.

For example, the pellets collected directly from the coast of Jeju and the like are washed, dried (e.g., lyophilized) and pulverized and pulverized, and then washed with water having a volume of about 2 to 20 times (for example, about 10 times) (For example, 1 hour to 1 day) at an extraction temperature of 20 to 100 ° C (for example, 70 ° C) with a solvent selected from among lower alcohols such as methanol, ethanol and butanol , Extraction with cold extraction, reflux cooling extraction or ultrasonic extraction (for example, hot water extraction), and then, if necessary, residue removal, filtration and concentration are added to prepare the extract of the present invention. Further, the extract obtained by concentration is dissolved in water, followed by fractionation with ethyl acetate or the like, and the ethyl acetate layer is dried under reduced pressure to obtain a fraction. The concentrated extract can be lyophilized and prepared in a powdered state.

In particular, the present invention provides the L (Ishige (cosmetics) comprising an extract of Okamurae as an active ingredient.

According to the present invention, new functional cosmetics capable of eliminating insomnia and nervous anxiety can be manufactured. For example, the sleep-inducing functional cosmetic of the present invention can be put to sleep before sleeping.

Specifically, the present invention has developed four kinds of cosmetics such as the following ones containing a Lard extract, and these products have proven to have a sleep inducing effect through clinical experiments.

As a first example, the water-surface-inducing functional cosmetic composition of the present invention is an essence type and is an essence type, and it is an essence type, and it is an essence type, such as purified water, cyclopentasiloxane, butyleneglycol, glycerin, biosaccharide gum-1, dimethicone, 10 dimethicone / vinyl dimethicone crosspolymer, polysorbate 20, hydroxyacetophenone, dimethicone / vinyl dimethicone crosspolymer, ammonium acryloyldimethyltaurate / vpicopolymer, tromethamine, carbomer , Lard extract, tocopheryl acetate, allantoin, sodium hyaluronate, dimethicone, disodium edetate and flavoring.

In addition to its excellent water-inducing function, this formulation enhances skin elasticity, moisturizes the skin sensitive to external influences, contains allantoic components, and improves the rough and rough skin. It has the effect of strengthening skin barrier.

As a second example, the water-surface-inducing functional cosmetic composition of the present invention is a cream-type composition and contains purified water, cetyl ethylhexanoate, glycerin, butylene glycol, 1,2-hexanediol, hydrogencarbonate poly (C6-14 olefin) , Cholesterol stearate, hydroxyacetophenone, acrylate / C10-30 alkyl acrylate crosspolymer, tromethamine, chamomile extract, chrysanthemum extract Extracts of flowers, flower extracts, dimethicone, matte extract, lettuce extract, lavender extract, Lard extract, tocopheryl acetate, xanthan gum, sodium hyaluronate, yam root extract, hydrolyzed corn starch, beta-glucan, sucrose , Disodium iodide, and fragrances.

In addition to its excellent sleep inducing function, this extract enhances skin elasticity, and contains lavender and chamomile extracts to prevent skin dryness during sleep, alleviate skin sedation and irritation, It contains saponin and allantoin to keep skin moist and smooth by protecting skin and forming natural moisturizing film.

As a third example, the water-surface-inducing functional cosmetic composition of the present invention is an all-in-one cream type, and can be used in various forms such as purified water, glycerin, hydroxyphenylurea, niacinamide, propanediol, squalane, ammonium acryloylmethyltaurate / / FFP-17/6 copolymer, butyleneglycol, dimethicone, phenyltrimethicone, triethyhexanone, dimethicone / vinyl dimethicone crosspolymer, succulent tea leaf extract, caprylic glycol, , Tocopheryl acetate, sodium hyaluronate, caprylic hydroxamic acid, sunflower seed oil, adenosine, Baiichi tea, agar, carrageenan extract, soybean oil, sodium starch octenyl succinate, maltodextrin, calcium pantothenate , Sodium ascorbyl phosphate, pyridoxine HCSEEL, Vicassian oil, tocopherol, silica, C12-14 pares-12, disodium iodide, phenoxyethanol and clophenethine It will be included.

In addition to its excellent water-inducing function, this product is blended with an oil base and a water base, so that it is fresh and light, so that hyaluronic acid and glycerin are quickly absorbed into the skin to provide a sufficient amount of water. It contains squalane and vitamin E Protects and protects the skin and gives it a nutritive sensation. It gives a moisturizing shine to the skin and gives it a smooth skin. It contains a citrus extract to calm the skin, increase the moisturizing power and revitalize the weary skin.

In a fourth aspect, the present invention relates to a water-insoluble functional cosmetic composition which is toner (toning water) type and contains purified water, ethanol, butyleneglycol, niacinamide, 1,2-hexanediol, glycereth- Extracts of chrysanthemum flowers, chamomile extracts, carbomes, extracts of chrysanthemum extract, chrysanthemum extract, chrysanthemum extract, chrysanthemum extract, Tromethamine, sodium hyaluronate, disodium iodide, clofenesin and fragrances.

In addition to its excellent water-inducing properties, this extract enhances skin elasticity, contains lavender and chamomile extracts to help revitalize the cycle of the skin cycle, helping to transform into a new skin layer, To prevent dryness of the skin and excellent moisturizing power, so as to calm the skin while sleeping and to reduce irritation.

In the four types of water-induced functional cosmetic compositions, 0.1 to 10% by weight (for example, 2% by weight) of the extract of L-Lactose as an active ingredient, 40 to 70% by weight (for example, 50% by weight) of purified water, To 50% by weight (e.g., equal to 48% by weight in total, respectively), but are not limited thereto.

In addition, the present invention can be applied to foods and medicines that require a sleep-inducing function. Thus a further aspect of the present invention L (Ishige a water-induced functional food composition and a water-induced functional pharmaceutical composition comprising an extract of Okamurae as an active ingredient.

There is no particular limitation on the kind of the food to which the extract of the present invention is added. For example, it includes all kinds of health foods in the ordinary sense such as drinks, tea, drink, alcoholic beverage and vitamin complex.

The food may contain various ingredients such as various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the food may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks, and these ingredients may be used independently or in combination.

In addition, the pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable additive. Examples of pharmaceutically acceptable additives include starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, calcium hydrogenphosphate, lactose, mannitol, sugar, arabic gum, cornstarch starch, powdered cellulose, But are not limited to, hydroxypropylcellulose, sodium starch glycolate, carnauba wax, synthetic aluminum silicate, stearic acid, magnesium stearate, aluminum stearate, calcium stearate, white sugar, dextrose, sorbitol and talc. Such an additive may be included in an amount of 1 to 50 parts by weight based on 100 parts by weight of the pharmaceutical composition.

The water-insoluble functional food composition and the water-surface-inducing functional pharmaceutical composition of the present invention may contain the extract of the present invention as an active ingredient in an amount of 0.1 to 90% by weight based on the total weight of the composition.

The sleep-inducing functional composition according to the present invention increases the expression of melatonin as well as the melatonin receptor (MT1), thereby exerting an excellent sleep-inducing effect.

In particular, the water-insoluble functional composition of the present invention can be suitably used as a material for functional cosmetics capable of solving insomnia and anxiety, and can also be used as a material for functional foods and medicines.

FIG. 1 is a graph showing the effect of Lard extract on cell viability.
Fig. 2 is a graph showing changes in expression of melatonin receptors by treatment with Lard extract.
FIG. 3 is a graph showing changes in body weight of a mouse according to the feeding of Lactobacillus extract.
FIG. 4 is a graph showing changes in the content of melatonin in mice according to the feeding of Lactobacillus extract.
FIG. 5 is a graph showing changes in brain melatonin receptors in mouse according to the feeding of Lactobacillus extract.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It is to be understood, however, that the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Example

(One) Manufacturing example

The tiles collected directly from nature such as the coast of Jeju are washed, lyophilized and pulverized and powdered, and then subjected to hot extraction at 70 ° C for 1 hour to 1 day (for example, 3 hours) in water having a volume of about 10 times the dry weight Next, the residue was removed and concentrated. If necessary, the extract was lyophilized and pulverized to prepare Lard extract to be used in the examples.

(2) Example  One

1, dimethicone, horseradish extract, 1,2-hexanediol, phage-10 dimethicone / vinyl dimethicone crosspolymer, polysorbate 20, cyclodextrin, , Hydroxyacetophenone, dimethicone / vinyl dimethicone crosspolymer, ammonium acryloyldimethyltaurate / vpicopolymer, tromethamine, carbomer, tallow extract, tocopheryl acetate, allantoin, sodium hyaluronate, di Methicone, disodium dithiophosphate and fragrance were uniformly mixed to prepare an essence type cosmetic product.

(3) Example  2

(C6-14 olefin), isoamyl laurate, cetearyl olivate, sorbitan olivate, sorbitan monooleate, sorbitan monooleate, sorbitan monooleate, glycerin, Lactic acid extract, Lactobacillus stearate, Lactobacillus stearate, Hydroxyacetophenone, Acrylate / C10-30 alkyl acrylate crosspolymer, Tromethamine, Chamomile extract, Chrysanthemum flower extract, Dimethicone, Extracts, tocopheryl acetate, xanthan gum, sodium hyaluronate, yam root extract, hydrolyzed corn starch, beta-glucan, sucrose, disodium dithiophosphate and fragrance are uniformly mixed to form a cream-type cosmetic .

(4) Example  3

But are not limited to, water, purified water, glycerin, hydroxyethanol, niacinamide, propanediol, squalane, ammonium acryloyldimethyltaurate / vpicopolymer, Phenyltrimethicone, trimethicone, dimethicone / vinyl dimethicone crosspolymer, succulent tea leaf extract, caprylic glycol, Lahore extract, tocopheryl acetate, sodium hyaluronate, caprylhydroxamic acid, Sodium ascorbyl phosphate, pyridoxine HCI CI, Vicassian oil, tocopherol, silica, sodium bicarbonate, sodium carboxymethylcellulose, sodium carboxymethylcellulose, sunflower seed oil, adenosine, Viechi tea, agar, carrageenan extract, soybean oil, sodium starch octenyl succinate, maltodextrin, calcium pantothenate, C12-14 pares-12, disodium iodide, phenoxyethanol and chlorphenesin were uniformly mixed to prepare an all-in-one cream type cosmetic.

(5) Example  4

Extracts such as water, purified water, ethanol, butyleneglycol, niacinamide, 1,2-hexanediol, glycereth-26, hyaluronic acid extract, hydroxyacetophenone, phage-40 hydrogenated castor oil, octyldodeces- , Panthenol, Lavender Extract, Lettuce Extract, Mattee Extract, Hydroxyethylcellulose, Chrysanthemum Flower Extract, Chamomile Extract, Carbomer, Tromethamine, Sodium Hyaluronate, Disodium Iodide, Clofenacin and Spices Were uniformly mixed to prepare a toner-type cosmetic.

Experimental conditions

(1) Cytotoxicity of cell cultures and extracts

In order to examine the effect of water extract on the sleep induction, we first determined the cytotoxicity - free concentration using an in vitro cell model.

For this purpose, C6 glioma cells were cultured in RPMI 1640 medium (10% fetal bovine serum, 1% penicillin-streptomycin), and C6 extracts were grown in CO ₂ incubator for 24 hours in 96-well plates. . After further culturing, the cytotoxicity was verified by measuring the absorbance using WST-1 cell proliferation kit (Cayman chemical, MI, USA).

(2) Verification of melatonin receptor expression in vitro )

The expression of melatonin receptors was examined in the mRNA level of C6 glioma cells after treatment with L - extracts.

For this purpose, 3 × 10 ⁴ C6 glioma cells were cultured in a 24-well plate, and then treated with the cell extract (0, 0.01, 0.05 mg / mL). After incubation for 24 hrs, total RNA was extracted using trizol reagent (Ambion, NY, USA). The expression of melatonin receptors 1 and 2 was verified by PCR after cDNA synthesis. The expression of the housekeeping gene, GAPDH gene, and the relative expression was verified. The PCR reaction was carried out at 94 ° C for 4 minutes, followed by 35 cycles of 95 ° C for 20 seconds, 60 ° C for 39 seconds, and 72 ° C for 5 minutes.

(3) Animal experiments for melatonin validation in vivo )

Sixteen male mice (C57BL6N, 6 weeks old) were purchased from an animal laboratory of Incheon University (22 ~ 23 ℃, relative humidity 55 ~ 60% After 12 weeks of light period, the mice were adapted to the breeding environment for 1 week before the experiment and the body weight was measured.

The extracts were administered once a day for 4 weeks on a 1 g / day / kg body weight basis and then administered orally at 5 pm. Control mice were orally administered the same volume (250 uL) of phosphate buffer saline (PBS) and body weights were measured at weekly intervals.

All animal experiments were conducted according to the guidelines of the Animal Experimentation Ethics Committee of the University of Incheon (INU-2016-07).

(4) Quantification of Melatonin in vivo )

To determine the changes of melatonin content in the extracts, 4 hours after the last sample feeding, blood samples were collected by cardiac blood collection and the serum was separated. Melatonin was used for quantification.

Melatonin was measured using a mouse melatonin ELISA kit (MyBioSource Co., CA, USA).

(5) Verification of melatonin receptor expression level in mouse brain in vivo )

The expression pattern of melatonin receptors in mouse brain after feeding of L - extract was examined by Western blotting of melanocortin receptors MT1 and MT2 after 4 weeks of feeding of L - extracts.

The brain tissues were lysed in cell lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mL NaCl, 5 mM EDTA, 1% (w / v) IGEPAL CA-630, 5 mM sodium pyrophosphate, 10 mM β-glycerophosphate, 1 mM phenylmethylsulfonyl fluoride) and the tissue was homogenized with a tissue homogenizer (Hanzhou Miu instruments Co., zhejing, China). The supernatant was centrifuged and the supernatant was used for the analysis.

The rabbit anti-MT1 antibody and rabbit anti-MT2-antibody (Origene, MD, USA) were used for the detection of mouse melatonin receptors MT1 and MT2, and goat anti-rabbit IgG-HRP (Santacruz, Antibody was used and finally developed on film using ECL solution (Biorad, Seoul, Korea).

(6) Statistical analysis

Test results were analyzed using statistical analysis program (SAS 9.1) and verified by student-t test method. The results were expressed as mean ± SD.

Experiment result

(1) Evaluation of cytotoxicity of extracts

Cell viability of the C6 glioma cell line was examined after treatment at the concentration to examine the cytotoxicity of the extract.

As shown in FIG. 1, the extracts of Lard influenced the survival rate of cells from the concentration of 0.1 mg / mL.

Therefore, in this experiment, the effects of 0.01 and 0.05 mg / mL of L. extract on the expression of melatonin receptors MT1 and MT2 were examined.

(2) The effect of Lactobacillus extract on melatonin receptor expression Influence  evaluation

In order to evaluate the effect of L-arabic extract on melatonin receptor expression, expression of mRNA levels of intracellular MT1 and MT2 in rat C6 glioma cell line was assayed by RT-PCR (0, 0.01, 0.05 mg / mL) .

As shown in FIG. 2, the expression of MT1 increases depending on the concentration of Lactobacillus extract (P <0.05). MT2 showed a slight increase at the level of 0.01 mg / mL of L. extract but not statistically significant (P> 0.05).

These results demonstrate that Lactobacillus extract possesses the ability to effectively increase the expression of MT1 in melatonin receptors, suggesting that melatonin-induced intracellular signaling may be more strongly promoted to promote sleep induction It is suggestive.

(3) The effect of Lactobacillus extract on melatonin expression Influence  evaluation( in vivo )

To investigate the changes in melatonin content by the treatment with L-elaborate, C57BL / 6 mice (n = 8) were fed 4 h of L. extract and compared with the amount of melatonin in the blood of control mice (PBS, n = 8) Induced melatonin expression.

As shown in FIG. 3, there was no significant difference in the weight change between the control group and the control group fed at 1 g / day / kg body weight level over 4 weeks.

This indirectly demonstrates that the concentration level of the saline roe extract does not act as a major toxic to the living body and has no side effects.

In addition, the blood was extracted after 4 weeks of feeding of Lactobacillus extract, and the content of melatonin in the serum was measured, and the result of comparison with the control group is shown in FIG.

As shown in FIG. 4, the 4-week feeding of Lactobacillus extract increased the melatonin content of the blood by about 12% (control: 585.56 ± 52.04 pg / mL, treated group: 654.14 ± 47.13 pg / P < 0.05).

(4) Measurement of Melatonin Receptor Expression in Mouse Brain Treated with Lard Extract in vivo )

To determine the changes of melatonin receptors according to the feeding of the extracts, the extracts were fed at a level of 1 g / day / kg body weight for 4 weeks. Mouse brain tissues were extracted and the expression levels of MT1 and MT2 in the granulosa were determined by western blotting (N = 4).

As shown in FIG. 5, MT1 expression was increased by about 2.2-fold (p <0.05) in the Lactobacillus casei extract group but not significantly in the MT2 expression group (p> 0.05).

These results are in agreement with the results (Fig. 2) of the melatonin receptor expression observed at the in vitro cell level, demonstrating that the Lactobacillus extract actually has the effect of increasing melatonin receptor expression in the body, It is once again demonstrated that induction of melatonin receptor expression can increase sleep induction.

(5) Human body application test result of sleep inducing functional cosmetics

Skin texture, moisturizing power and sleep aid effect of four kinds of water-induced functional cosmetics manufactured according to Examples 1 to 4 were evaluated by the KC Skin Clinical Research Center Co., Ltd. The results are shown in Table 2 same.

[Table 2]

Review results

The present invention proves that Lepidoptera extract is effective in inducing expression of melatonin receptors and melatonin which are molecular indicators important for the water-induced induction effect.

Melatonin and melatonin receptor - inducing effects were verified by in vitro and in vivo experiments in order to verify the sleep - inducing effect of extracts.

Although most of the materials known to have sleep inducing effects have proved whether or not they can induce the expression of melatonin, they have proved whether or not the material has a sleep inducing effect. However, the L-extract used in the present invention induces melatonin expression But also to induce the expression of melatonin receptors.

Considering that the melatonin receptor plays a role in amplifying and delivering the signal induced by melatonin to the cells, the increase of the melatonin receptor seems to increase the actual sleep inducing effect several times in the presence of the same amount of melatonin.

Melatonin and melatonin receptors have been recognized as effective targets for the treatment of diseases such as insomnia and anxiety. Therefore, the L-extract according to the present invention is likely to develop into a material and a new drug effective for the treatment of diseases such as insomnia and anxiety.

With the development of society, the increase of the elderly population is evident all over the world, and the demand for material and product development that can heal and inhibit geriatric diseases is expected to increase exponentially.

In particular, at present, as a representative example of geriatric diseases, the population showing insomnia and nervous anxiety symptoms is increasing, the present invention is expected to be used as a new material for cosmetics and foods having a sleep inducing function, thereby causing a large ripple effect.

Claims (10)

Ishige okamurae ) extract as an active ingredient,
A sleep-inducing functional composition.
The method according to claim 1,
Characterized in that said Lard extract promotes the expression of melatonin.
A sleep-inducing functional composition.
The method according to claim 1,
Lt; RTI ID = 0.0 &gt; (MT1) &lt; / RTI &gt;
A sleep-inducing functional composition.
Ishige okamurae ) extract as an active ingredient,
A water-induced functional cosmetic composition.
5. The method of claim 4,
The cosmetic composition is an essence type,
1, dimethicone, horseradish extract, 1,2-hexanediol, phage-10 dimethicone / vinyl dimethicone crosspolymer, polysorbate 20, cyclodextrin, , Hydroxyacetophenone, dimethicone / vinyl dimethicone crosspolymer, ammonium acryloyldimethyltaurate / vpicopolymer, tromethamine, carbomer, tallow extract, tocopheryl acetate, allantoin, sodium hyaluronate, di Methicone, disodium edetate, and flavoring. &Lt; RTI ID = 0.0 &gt;
A water-induced functional cosmetic composition.
5. The method of claim 4,
The cosmetic composition is a cream type,
(C6-14 olefin), isoamyl laurate, cetearyl olivate, sorbitan olivate, sorbitan monooleate, sorbitan monooleate, sorbitan monooleate, glycerin, Lactic acid extract, Lactobacillus stearate, Lactobacillus stearate, Hydroxyacetophenone, Acrylate / C10-30 alkyl acrylate crosspolymer, Tromethamine, Chamomile extract, Chrysanthemum flower extract, Dimethicone, Characterized in that it comprises an extract, tocopheryl acetate, xanthan gum, sodium hyaluronate, yam root extract, hydrolyzed corn starch, beta-glucan, sucrose,
A water-induced functional cosmetic composition.
5. The method of claim 4,
The cosmetic composition is an all-in-one cream type,
But are not limited to, water, purified water, glycerin, hydroxyethanol, niacinamide, propanediol, squalane, ammonium acryloyldimethyltaurate / vpicopolymer, Phenyltrimethicone, trimethicone, dimethicone / vinyl dimethicone crosspolymer, succulent tea leaf extract, caprylic glycol, Lahore extract, tocopheryl acetate, sodium hyaluronate, caprylhydroxamic acid, Sodium ascorbyl phosphate, pyridoxine HCI CI, Vicassian oil, tocopherol, silica, sodium bicarbonate, sodium carboxymethylcellulose, sodium carboxymethylcellulose, sunflower seed oil, adenosine, Viechi tea, agar, carrageenan extract, soybean oil, sodium starch octenyl succinate, maltodextrin, calcium pantothenate, C12-14 pares-12, disodium iodide, phenoxyethanol and chlorphenesin.
A water-induced functional cosmetic composition.
5. The method of claim 4,
The cosmetic composition is a toner type,
Extracts such as water, purified water, ethanol, butyleneglycol, niacinamide, 1,2-hexanediol, glycereth-26, hyaluronic acid extract, hydroxyacetophenone, phage-40 hydrogenated castor oil, octyldodeces- , Panthenol, Lavender Extract, Lettuce Extract, Mattee Extract, Hydroxyethylcellulose, Chrysanthemum Flower Extract, Chamomile Extract, Carbomer, Tromethamine, Sodium Hyaluronate, Disodium Iodide, Clofenacin and Spices &Lt; / RTI &gt;
A water-induced functional cosmetic composition.
Ishige okamurae ) extract as an active ingredient,
A sleep-inducing functional food composition.
Ishige okamurae ) extract as an active ingredient,
A sleep-inducing functional pharmaceutical composition.

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