KR102588771B1 - COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING - Google Patents
COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING Download PDFInfo
- Publication number
- KR102588771B1 KR102588771B1 KR1020210066226A KR20210066226A KR102588771B1 KR 102588771 B1 KR102588771 B1 KR 102588771B1 KR 1020210066226 A KR1020210066226 A KR 1020210066226A KR 20210066226 A KR20210066226 A KR 20210066226A KR 102588771 B1 KR102588771 B1 KR 102588771B1
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- colpomenia
- pharmaceutical composition
- composition
- estrogen
- Prior art date
Links
- 239000000284 extract Substances 0.000 title claims abstract description 127
- 229940011871 estrogen Drugs 0.000 title claims abstract description 56
- 239000000262 estrogen Substances 0.000 title claims abstract description 56
- 239000000203 mixture Substances 0.000 title claims abstract description 39
- 239000004480 active ingredient Substances 0.000 title claims abstract description 19
- 208000024891 symptom Diseases 0.000 title abstract description 15
- 241000004832 Colpomenia sinuosa Species 0.000 title abstract description 7
- 241001126869 Colpomenia peregrina Species 0.000 title abstract description 6
- 230000001603 reducing effect Effects 0.000 title description 3
- 235000013305 food Nutrition 0.000 claims abstract description 22
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 19
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 18
- 201000010099 disease Diseases 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 57
- 230000003247 decreasing effect Effects 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 206010046788 Uterine haemorrhage Diseases 0.000 claims description 6
- 206010006187 Breast cancer Diseases 0.000 claims description 4
- 208000026310 Breast neoplasm Diseases 0.000 claims description 4
- 208000030136 Marchiafava-Bignami Disease Diseases 0.000 claims description 4
- 208000029725 Metabolic bone disease Diseases 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- 208000005641 Adenomyosis Diseases 0.000 claims description 3
- 206010008263 Cervical dysplasia Diseases 0.000 claims description 3
- 206010014733 Endometrial cancer Diseases 0.000 claims description 3
- 206010014759 Endometrial neoplasm Diseases 0.000 claims description 3
- 201000009273 Endometriosis Diseases 0.000 claims description 3
- 206010033128 Ovarian cancer Diseases 0.000 claims description 3
- 206010061535 Ovarian neoplasm Diseases 0.000 claims description 3
- 208000002495 Uterine Neoplasms Diseases 0.000 claims description 3
- 206010046798 Uterine leiomyoma Diseases 0.000 claims description 3
- 206010046799 Uterine leiomyosarcoma Diseases 0.000 claims description 3
- 206010046910 Vaginal haemorrhage Diseases 0.000 claims description 3
- 208000007951 cervical intraepithelial neoplasia Diseases 0.000 claims description 3
- 201000009274 endometriosis of uterus Diseases 0.000 claims description 3
- 201000010260 leiomyoma Diseases 0.000 claims description 3
- 208000007106 menorrhagia Diseases 0.000 claims description 3
- 239000012046 mixed solvent Substances 0.000 claims description 3
- 206010046766 uterine cancer Diseases 0.000 claims description 3
- 125000004432 carbon atom Chemical group C* 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 38
- 239000003814 drug Substances 0.000 abstract description 16
- 229940079593 drug Drugs 0.000 abstract description 13
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 abstract description 8
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 abstract description 6
- 229960005309 estradiol Drugs 0.000 abstract description 6
- 229930182833 estradiol Natural products 0.000 abstract description 6
- 230000002265 prevention Effects 0.000 abstract description 5
- 230000006872 improvement Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 16
- 238000010171 animal model Methods 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 241000199919 Phaeophyceae Species 0.000 description 8
- 235000013376 functional food Nutrition 0.000 description 8
- 230000036541 health Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 230000026731 phosphorylation Effects 0.000 description 8
- 238000006366 phosphorylation reaction Methods 0.000 description 8
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 241001474374 Blennius Species 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 239000000090 biomarker Substances 0.000 description 7
- 210000000988 bone and bone Anatomy 0.000 description 7
- 230000004097 bone metabolism Effects 0.000 description 7
- 239000000546 pharmaceutical excipient Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 6
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 6
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 6
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 6
- 102000008108 Osteoprotegerin Human genes 0.000 description 6
- 108010035042 Osteoprotegerin Proteins 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 229940028334 follicle stimulating hormone Drugs 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 6
- XXUPLYBCNPLTIW-UHFFFAOYSA-N octadec-7-ynoic acid Chemical compound CCCCCCCCCCC#CCCCCCC(O)=O XXUPLYBCNPLTIW-UHFFFAOYSA-N 0.000 description 6
- 210000001672 ovary Anatomy 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 230000036760 body temperature Effects 0.000 description 5
- 210000004556 brain Anatomy 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000012790 confirmation Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- -1 olive oil Chemical compound 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- SFLSHLFXELFNJZ-QMMMGPOBSA-N (-)-norepinephrine Chemical compound NC[C@H](O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-QMMMGPOBSA-N 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 206010027304 Menopausal symptoms Diseases 0.000 description 4
- 208000001132 Osteoporosis Diseases 0.000 description 4
- 102000014128 RANK Ligand Human genes 0.000 description 4
- 108010025832 RANK Ligand Proteins 0.000 description 4
- 235000013361 beverage Nutrition 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000009245 menopause Effects 0.000 description 4
- 229960002748 norepinephrine Drugs 0.000 description 4
- SFLSHLFXELFNJZ-UHFFFAOYSA-N norepinephrine Natural products NCC(O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-UHFFFAOYSA-N 0.000 description 4
- 238000009806 oophorectomy Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 229940076279 serotonin Drugs 0.000 description 4
- 210000000689 upper leg Anatomy 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 206010016825 Flushing Diseases 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 102220497176 Small vasohibin-binding protein_T47D_mutation Human genes 0.000 description 3
- 206010047791 Vulvovaginal dryness Diseases 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 230000029142 excretion Effects 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 229930014626 natural product Natural products 0.000 description 3
- 239000002858 neurotransmitter agent Substances 0.000 description 3
- 235000012149 noodles Nutrition 0.000 description 3
- 239000006072 paste Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000829 suppository Substances 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- 241001312221 Anthurium Species 0.000 description 2
- 208000019901 Anxiety disease Diseases 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 2
- 229940122361 Bisphosphonate Drugs 0.000 description 2
- 238000011740 C57BL/6 mouse Methods 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 2
- 241000218691 Cupressaceae Species 0.000 description 2
- 235000017788 Cydonia oblonga Nutrition 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 208000031226 Hyperlipidaemia Diseases 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 208000017657 Menopausal disease Diseases 0.000 description 2
- 208000008589 Obesity Diseases 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 244000299461 Theobroma cacao Species 0.000 description 2
- 208000009911 Urinary Calculi Diseases 0.000 description 2
- 206010046914 Vaginal infection Diseases 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 230000036506 anxiety Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 208000029078 coronary artery disease Diseases 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012156 elution solvent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 239000005417 food ingredient Substances 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 230000003054 hormonal effect Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 235000021109 kimchi Nutrition 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000027939 micturition Effects 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000020824 obesity Nutrition 0.000 description 2
- 239000002674 ointment Substances 0.000 description 2
- 230000011164 ossification Effects 0.000 description 2
- 239000000123 paper Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 208000019116 sleep disease Diseases 0.000 description 2
- 235000011888 snacks Nutrition 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000003765 sweetening agent Substances 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 235000012222 talc Nutrition 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 208000008281 urolithiasis Diseases 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 235000006491 Acacia senegal Nutrition 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 208000006386 Bone Resorption Diseases 0.000 description 1
- 206010065687 Bone loss Diseases 0.000 description 1
- 229940078581 Bone resorption inhibitor Drugs 0.000 description 1
- 102000055006 Calcitonin Human genes 0.000 description 1
- 108060001064 Calcitonin Proteins 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 235000007862 Capsicum baccatum Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 241001126871 Colpomenia Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
- 206010014522 Embolism venous Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 239000004606 Fillers/Extenders Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 208000026139 Memory disease Diseases 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 206010028813 Nausea Diseases 0.000 description 1
- 206010029216 Nervousness Diseases 0.000 description 1
- 206010030216 Oesophagitis Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 206010040799 Skin atrophy Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 206010071018 Urogenital atrophy Diseases 0.000 description 1
- 201000008100 Vaginitis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 241000272195 Vultur Species 0.000 description 1
- 206010056530 Vulvovaginal pruritus Diseases 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000002386 air freshener Substances 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000001164 bioregulatory effect Effects 0.000 description 1
- 150000004663 bisphosphonates Chemical class 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 208000002352 blister Diseases 0.000 description 1
- 230000024279 bone resorption Effects 0.000 description 1
- 230000008416 bone turnover Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- 229960004015 calcitonin Drugs 0.000 description 1
- BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- 235000012241 calcium silicate Nutrition 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 239000001728 capsicum frutescens Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 229940109239 creatinine Drugs 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 235000015140 cultured milk Nutrition 0.000 description 1
- 201000003146 cystitis Diseases 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 235000011869 dried fruits Nutrition 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 208000006881 esophagitis Diseases 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
- 229940093471 ethyl oleate Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 235000019985 fermented beverage Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000021189 garnishes Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 210000004349 growth plate Anatomy 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000003688 hormone derivative Substances 0.000 description 1
- 238000002657 hormone replacement therapy Methods 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000007913 intrathecal administration Methods 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- VMPHSYLJUKZBJJ-UHFFFAOYSA-N lauric acid triglyceride Natural products CCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCC)COC(=O)CCCCCCCCCCC VMPHSYLJUKZBJJ-UHFFFAOYSA-N 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 229960003511 macrogol Drugs 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 208000013465 muscle pain Diseases 0.000 description 1
- 239000007922 nasal spray Substances 0.000 description 1
- 229940097496 nasal spray Drugs 0.000 description 1
- 230000008693 nausea Effects 0.000 description 1
- 239000012457 nonaqueous media Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 239000006186 oral dosage form Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 230000027758 ovulation cycle Effects 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 210000001316 polygonal cell Anatomy 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 235000020991 processed meat Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000001076 sarcopenia Diseases 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 235000021264 seasoned food Nutrition 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 239000002884 skin cream Substances 0.000 description 1
- 208000022925 sleep disturbance Diseases 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 235000013322 soy milk Nutrition 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 230000001457 vasomotor Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 208000004043 venous thromboembolism Diseases 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/03—Phaeophycota or phaeophyta (brown algae), e.g. Fucus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/12—Drugs for genital or sexual disorders; Contraceptives for climacteric disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
- A61P5/30—Oestrogens
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/302—Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/202—Algae extracts
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Endocrinology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Diabetes (AREA)
- Epidemiology (AREA)
- Reproductive Health (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 불레기말 (Colpomenia sinuosa) 또는 반질불레기말 (Colpomenia peregrina Sauvageau) 추출물을 유효성분으로 포함하는 에스트로겐 수치 변화와 관련된 질환의 조절, 억제, 예방, 개선 또는 치료용 약학적 조성물에 관한 것으로서, 보다 상세하게는 에스트라디올 특이적 세포모델에서 에스트라디올 증가 효과를 가지는 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는 에스트로겐 수치 변화와 관련된 증상의 조절, 예방 또는 치료용 약학적 조성물, 개선용 식품 조성물, 의약외품 조성물에 관한 것이다. The present invention relates to a pharmaceutical composition for controlling, suppressing, preventing, improving or treating diseases related to changes in estrogen levels, containing an extract of Colpomenia sinuosa or Colpomenia peregrina Sauvageau as an active ingredient. Specifically, a pharmaceutical composition for the control, prevention or treatment of symptoms related to changes in estrogen levels, and a food composition for the improvement, containing as an active ingredient an extract of Malt vulgaris or Malt vulgaris, which has an estradiol-increasing effect in an estradiol-specific cell model. , relates to quasi-drug compositions.
Description
본 발명은 에스트로겐 수치 감소를 개선시키는 효과를 나타내는 불레기말 (Colpomenia sinuosa) 또는 반질불레기말 (Colpomenia peregrina Sauvageau) 추출물을 유효성분으로 포함하는 에스트로겐 감소에 의한 증상 완화 및 개선용 조성물에 관한 것이다. The present invention relates to a composition for alleviating and improving symptoms caused by a decrease in estrogen, comprising as an active ingredient an extract of Colpomenia sinuosa or Colpomenia peregrina Sauvageau, which has the effect of improving the decrease in estrogen levels.
여성의 경우 난소의 기능저하로 인해 에스트로겐 수준이 감소하게 되면, 체내 에스트로겐 수치 감소와 함께 다양한 증상이 나타나는데, 대표적인 것으로 안면 홍조, 질 건조증이 있고, 수면 장애, 우울, 불안 등의 심리적 증상, 심혈관질환 및 골다공증 등의 만성질환 위험이 증가된다. 에스트로겐 수치가 감소하는 시기의 여성 50% 이상에서는 비뇨생식기 위축으로 인한 질 건조증, 가려움증 등의 증상을 경험하는 것으로 알려져 있으며, 에스트로겐 분비가 중단되는 시기의 여성 75%에서는 혈관운동 증상 중 특히 안면홍조를 대부분의 여성이 1-2년 내외로 경험하며, 10년 이상 증상이 지속되기도 한다. 또한, 점차 월경주기가 불규칙해지거나 출혈 양에도 변화가 오기 시작하며, 여러 가지 부정적인 신체적 변화를 경험하게 되고 에스트로겐의 분비가 중단되는 시기 이후부터는 골소실로 인한 골다공증 현상이 두드러지게 나타난다. In women, when estrogen levels decrease due to decreased ovarian function, various symptoms appear along with the decrease in estrogen levels in the body. Representative examples include facial flushing, vaginal dryness, sleep disorders, psychological symptoms such as depression and anxiety, and cardiovascular disease. and the risk of chronic diseases such as osteoporosis increases. It is known that more than 50% of women during a period when estrogen levels decrease experience symptoms such as vaginal dryness and itching due to urogenital atrophy, and 75% of women during a period when estrogen secretion ceases experience vasomotor symptoms, especially facial flushing. Most women experience it for about 1-2 years, and symptoms may persist for 10 years or more. In addition, the menstrual cycle gradually becomes irregular and the amount of bleeding begins to change, and various negative physical changes are experienced. After the period when estrogen secretion stops, the phenomenon of osteoporosis due to bone loss becomes noticeable.
이러한 에스트로겐의 분비가 중단된 이후 문제를 감소시키기 위하여 합성 에스트로겐을 포함한 호르몬 대체요법이 사용되고 있으나, 장기간의 추적연구에서 유방암, 정맥혈전색전증 뿐 아니라 관상동맥질환, 뇌졸중 등을 오히려 증가시키는 것으로 보고되고 있으며, 대표적인 칼슘제제의 경우 필요 이상의 칼슘을 섭취할 경우 칼슘 배설의 증가로 인하여 요로결석이 발생할 수 있으므로 요로결석의 병력이 있는 경우 주의를 요한다. 또한, 골 흡수 억제제로 사용하고 있는 대표적인 약물로 estrogen의 경우 관상동맥과 뇌졸중 등의 위험과 심혈관계에 대한 위험이 제기되고 있으며, Calcitonin의 경우 주사 혹은 비강분무를 하여야 한다는 점과 장기간 사용 시 내성이 생길 수 있으므로 몇 개월씩 휴식기간이 필요하다. 대표적인 치료제인 Bisphosphonate의 경우 위장관 증상으로 오심, 구토, 소화불량 등이 있을 수 있고 아미노-비스포스포네이트의 경우 식도염을 유발할 수 있어서 큰 컵으로 한 컵 이상의 맹물과 함께 복용하고 적어도 30분에서 1시간 이상 눕지 않도록 하는 것을 권장하는 등 복용에 주의를 요한다. 최근 개발되어 치료제로 널리 쓰이고 있는 Strontium의 경우 가장 흔한 부작용으로는 설사와 또한 일부 환자에서 정상 상한치의 2배가 넘는 creatinine kinase 수치가 관찰되고 있다. 따라서 여성의 삶의 질을 저하시키는 중요한 요인이 되는 에스트로겐의 감소 또는 중단으로 인한 증상을 완화시키고 만성질환의 발생을 예방할 수 있도록 하는 관리 방안의 제시가 필요시 되며, 이러한 합성호르몬 치료에 의한 부작용을 줄일 수 있는 천연물 유래의 에스트로겐의 감소 또는 중단으로 인한 장애 개선용 소재에 대한 개발 필요성이 증대되고 있다.Hormone replacement therapy, including synthetic estrogen, is being used to reduce these problems after the secretion of estrogen is stopped. However, in long-term follow-up studies, it is reported that it actually increases not only breast cancer and venous thromboembolism, but also coronary artery disease and stroke. , In the case of representative calcium preparations, if more calcium is consumed than necessary, urinary stones may occur due to increased calcium excretion, so caution is required if there is a history of urinary stones. In addition, estrogen, a representative drug used as a bone resorption inhibitor, raises the risk of coronary artery disease and stroke, as well as a risk to the cardiovascular system. Calcitonin requires injection or nasal spray and is resistant to long-term use. This can occur, so a break of several months is needed. Bisphosphonate, a representative treatment, can cause gastrointestinal symptoms such as nausea, vomiting, and indigestion, and amino-bisphosphonate can cause esophagitis, so take it in a large cup with at least a glass of plain water and avoid lying down for at least 30 minutes to 1 hour. Caution is required when taking it. In the case of Strontium, which was recently developed and widely used as a treatment, the most common side effects are diarrhea and creatinine kinase levels exceeding twice the upper limit of normal are observed in some patients. Therefore, it is necessary to suggest a management plan that can alleviate symptoms caused by the reduction or cessation of estrogen, which is an important factor in reducing women's quality of life, and prevent the occurrence of chronic diseases, and prevent side effects from such synthetic hormone treatment. There is an increasing need to develop materials for improving disorders caused by the reduction or cessation of estrogen derived from natural products that can be reduced.
한편, 불레기말 (Colpomenia sinuosa) 및 반질불레기말 (Colpomenia peregrina Sauvageau)는 고리매과의 갈조류로 우리나라의 제주도 및 전국 각지에 널리 분포해 있는 자생 해조류이며 구상 또는 불규칙한 모양으로 직경 4~10cm 정도이며 몸 구조는 피층은 1~2층의 정방형 또는 다각형 세포로 되어있고 내층은 2~5층의 둥근 세포로 되어있다. 최근 불레기말 및 반질불레기말의 활용가능성에 대한 지속적인 연구가 이루어지고 있다. On the other hand, Colpomenia sinuosa and Colpomenia peregrina Sauvageau are brown algae of the Cypress family and are native seaweeds widely distributed on Jeju Island and throughout the country in Korea. They are spherical or irregular in shape, about 4 to 10 cm in diameter, and have a body structure. The cortex is made of 1-2 layers of square or polygonal cells, and the inner layer is made of 2-5 layers of round cells. Recently, continuous research is being conducted on the feasibility of using Bulegimal and Banjil Bulegimal.
본 발명자들은 에스트로겐의 수치 감소 또는 중단으로 인한 증상의 예방 및 치료 효과가 있는 해양생물자원을 찾기 위하여 노력한 결과, 해조류 중 불레기말 (Colpomenia sinuosa) 및 반질불레기말 (Colpomenia peregrina Sauvageau) 추출물이 독성을 가지지 않고, 에스트라디올 특이적 세포모델에서 에스트라디올 수치를 증가시키는 효과를 나타내는 것을 확인함으로써, 본 발명을 완성하였다. The present inventors have made efforts to find marine biological resources that are effective in preventing and treating symptoms caused by reduction or cessation of estrogen levels, and as a result, extracts from seaweeds such as Colpomenia sinuosa and Colpomenia peregrina Sauvageau are not toxic. The present invention was completed by confirming that it had the effect of increasing estradiol levels in an estradiol-specific cell model.
본 발명의 하나의 목적은 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 조절, 예방, 개선 또는 치료용 약학적 조성물을 제공하는 것이다.One object of the present invention is to provide a pharmaceutical composition for controlling, preventing, improving, or treating estrogen levels, which contains extracts of the horse vulgaris or banjil vulgaris as an active ingredient.
본 발명의 다른 목적은 상기 조성물의 용도를 제공하는 것이다.Another object of the present invention is to provide a use for the composition.
본 발명의 또 다른 목적은 상기 약학적 조성물을 에스트로겐 수치 감가 의심 개체에 투여하는 단계를 포함하는, 에스트로겐 수치 감가의 치료방법을 제공하는 것이다.Another object of the present invention is to provide a method of treating decreased estrogen levels, comprising administering the pharmaceutical composition to a subject suspected of having decreased estrogen levels.
본 발명의 또 다른 목적은 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감가의 조절, 예방 또는 개선용 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide a food composition for controlling, preventing, or improving the decrease in estrogen levels, which contains extracts of the vulgaris or banjil vulgaris as an active ingredient.
본 발명의 또 다른 목적은 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감가의 조절, 예방 또는 개선용 의약외품 조성물을 제공하는 것이다.Another object of the present invention is to provide a quasi-drug composition for controlling, preventing, or improving the decrease in estrogen levels, which contains extracts of the horse vulgaris or banjil vulgaris as an active ingredient.
본 발명은 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감소 관련 증상의 조절, 예방, 개선 또는 치료용 약학적 조성물에 관한 것으로, 에스트라디올 특이적 세포모델에서 에스트로겐 수치 개선효과가 있어 유용하게 이용될 수 있다. 특히, 불레기말 또는 반질불레기말 추출물은 일부 해조류 추출물과 달리 독성이 없고, 천연물에서 유래되어 부작용이 없이 상기 질환의 치료에 이용될 수 있는 이점이 있다.The present invention relates to a pharmaceutical composition for controlling, preventing, ameliorating or treating symptoms related to decreased estrogen levels, which contains an extract of Malt vulgaris or Banjil vulgaris as an active ingredient, and has an effect of improving estrogen levels in an estradiol-specific cell model. It can be usefully used. In particular, unlike some seaweed extracts, the extract of the seaweed or banjil seaweed has the advantage of being non-toxic and derived from a natural product, so that it can be used to treat the above diseases without side effects.
도 1 은 불레기말 또는 반질불레기말의 에탄올 함량 별 추출물의 세포독성 (A) 및 에스트로겐 활성 변화 (B)를 확인한 결과이다.
도 2는 불레기말의 추출물에 의한 에스트로겐 신호전달 분자의 인산화 조절 효과를 확인한 것으로, 도 2 (A)는 MCF-7 세포에서, 도 2 (B)는 T47D 세포에서 확인한 것이다.
도 3은 동물 모델에 반질불레기말 추출물 등을 처리하였을 때 나타나는 체온변화를 확인한 결과이다.
도 4는 반질불레기말 추출물의 처리 농도에 따른 난소적출 여성 갱년기 동물모델에서 골대사와 관련된 실험결과로서, 도 4 (A)는 반질불레기말 추출물의 처리 농도에 따른 난소적출 여성 갱년기 동물모델에서 대퇴골 micro CT 이미지, 도 4 (B) 는 bone volume/total volume (BV/TV), 도 4 (C) 는 trabecular thickness (Tb.Th), 도 4 (D)는 trabecular number (Tb.N) 및 도 4 (E)는 trabecular bone mineral density (BMD)의 측정 결과를 나타낸 것이다.
도 5는 반질불레기말 추출물의 처리 농도에 따른 여성 갱년기 동물모델에서 골대사 바이오마커 변화를 확인한 결과이다. 구체적으로, 도 5 (A)는 RANKL, 도 5 (B)는 osteoprotegerin (OPG) 및 도 5 (C)는 RANKL/OPG 비율의 ELISA 분석 결과를 나타낸 것이다.
도 6은 반질불레기말 추출물의 처리 농도에 따른 여성 갱년기 동물모델에서 골대사 바이오마커 변화 및 호르몬 변화를 확인한 결과이다. 구체적으로 도 6 (A)는 alkaline phosphatase (ALP) 및 도 6 (B)는 CTX의 주요 바이오마커 분석 단계에서 ELISA 분석 결과를 나타낸 것이고, 도 6 (C)는 follicle stimulating horone (FSH)의 ELISA 분석 결과를 나타낸 것이다.
도 7은 반질불레기말 추출물의 난소적출 마우스모델의 뇌 전체의 신경전달 물질인 serotonin과 norepinephrine 농도별 활성에 관한 것이다.
도 8는 불레기말 또는 반질불레기말 추출물과 다른 갈조류 추출물의 활성을 비교한 결과이다. Figure 1 shows the results of confirming the cytotoxicity (A) and changes in estrogen activity (B) of extracts of Bolegifolia or Banjil Bolegifolia depending on the ethanol content.
Figure 2 confirms the effect of the extract of vulgaris horse on phosphorylation regulation of estrogen signaling molecules, with Figure 2 (A) confirming this in MCF-7 cells and Figure 2 (B) confirming this in T47D cells.
Figure 3 shows the results of confirming the change in body temperature that appears when an animal model is treated with Banjilbulregi extract, etc.
Figure 4 shows the results of an experiment related to bone metabolism in an ovariectomized female menopausal animal model according to the treatment concentration of the Banjilbulae horse extract, and Figure 4 (A) shows the femur micro bone metabolism in the ovariectomized female menopausal animal model according to the treatment concentration of the Banjil bullae horse extract. CT image, Figure 4 (B) is bone volume/total volume (BV/TV), Figure 4 (C) is trabecular thickness (Tb.Th), Figure 4 (D) is trabecular number (Tb.N), and Figure 4 (E) shows the measurement results of trabecular bone mineral density (BMD).
Figure 5 shows the results of confirming changes in bone metabolism biomarkers in a female menopausal animal model according to the treatment concentration of Banjilbulregi horse extract. Specifically, Figure 5 (A) shows the results of ELISA analysis of RANKL, Figure 5 (B) shows osteoprotegerin (OPG), and Figure 5 (C) shows the RANKL/OPG ratio.
Figure 6 shows the results of confirming changes in bone metabolism biomarkers and hormonal changes in a female menopausal animal model according to the treatment concentration of Banjilbulregi horse extract. Specifically, Figure 6 (A) shows the ELISA analysis results at the main biomarker analysis stage of alkaline phosphatase (ALP) and Figure 6 (B) CTX, and Figure 6 (C) shows the ELISA analysis of follicle stimulating hormone (FSH). It shows the results.
Figure 7 relates to the activity of serotonin and norepinephrine, which are neurotransmitters, in the entire brain of an ovariectomized mouse model of the extract of the end of Banjilbulregi by concentration.
Figure 8 shows the results of comparing the activity of extracts from Malt vulgare or Banjil vulgaris and other brown algae extracts.
상기 목적을 달성하기 위한 본 발명의 하나의 양태는 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감소에 의한 조절, 개선 또는 치료용 약학적 조성물 및 상기 조성물의 용도를 제공한다. One aspect of the present invention for achieving the above object provides a pharmaceutical composition for controlling, improving, or treating a decrease in estrogen levels, which contains an extract of the vulgaris or banjil vulgaris as an active ingredient, and a use of the composition.
본 발명에서 용어, “불레기말 (Colpomenia sinuosa)"은 고리매과의 갈조식물로 당뇨, 고지혈증, 비만 등 대사 질환에 대해 효과적이라는 것이 보고되어 있다. 그러나 불레기말 추출물이 에스트로겐 수치 감소의 조절, 치료 용도에 대해서는 알려진 바 없으며, 본 발명자들에 의하여 최초로 규명되었다. 본 발명에서 불레기말은 상업적으로 판매되는 것을 구입하거나, 자연에서 채취된 것을 사용할 수 있다.In the present invention, the term “ Colpomenia sinuosa ” is a brown algae of the Colpomenia family and has been reported to be effective for metabolic diseases such as diabetes, hyperlipidemia, and obesity. However, the Colpomenia sinuosa extract is used for controlling and treating decreased estrogen levels. There is nothing known about it, and it was first identified by the present inventors. In the present invention, the Buleggi mal can be purchased commercially or used when collected from nature.
본 발명에서 용어, "반질불레기말(Colpomenia peregrina Sauvageau)"는 고리매과의 갈조식물로, 근감소증 예방 및 치료, 또는 당뇨병 치료에 효과적으로 보고되어 있다. 그러나 반질불레기말 추출물이 에스트로겐 수치 감소의 조절, 치료 용도에 대해서는 알려진 바 없으며, 본 발명자들에 의하여 최초로 규명되었다. 본 발명에서 불레기말은 상업적으로 판매되는 것을 구입하거나, 자연에서 채취된 것을 사용할 수 있다. In the present invention, the term " Colpomenia peregrina Sauvageau" is a brown algae of the Cypress family and has been reported to be effective in preventing and treating sarcopenia or treating diabetes. However, there is no known use of the Banjilbulregi extract for controlling or treating decreased estrogen levels, and it was first identified by the present inventors. In the present invention, the vulgaris can be purchased commercially or collected from nature.
본 발명에서 용어, "불레기말 또는 반질불레기말 추출물"은 불레기말 또는 반질불레기말을 추출하여 수득한 추출물을 의미한다. 상기 추출물은 불레기말 또는 반질불레기말을 추출 처리에 의하여 얻어지는 모든 결과물을 의미하는 것으로서, 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성가능한 모든 제형의 추출물을 포함한다.In the present invention, the term "Bulegimal or Banjilbulegimal extract" refers to an extract obtained by extracting Bulleggimal or Banjilbulegimal. The extract refers to all the results obtained by extracting and processing the dried or half-baked dried fruit, such as extract, diluted or concentrated liquid of the extract, dried product obtained by drying the extract, crude or purified extract, or mixtures thereof, etc. , includes the extract itself and all formulations of the extract that can be formed using the extract.
상기 불레기말 또는 반질불레기말 추출물은 건조 불레기말 또는 반질불레기말 중량의 약 2 내지 20배, 구체적으로는, 약 3 내지 5배에 달하는 부피의 물, 메탄올, 에탄올 또는 부탄올 등과 같은 탄소수 1(C1) 내지 4(C4)의 저급 알코올의 극성 용매 또는 이들의 혼합용매를 용출 용매로써 사용할 수 있다. 상기 용출 용매는 구체적으로는 30 내지 95% 에탄올, 30 내지 90% 에탄올, 또는 30 내지 80% 에탄올일 수 있으며, 보다 구체적으로는 30 내지 70% 에탄올, 보다 더 구체적으로는 50 내지 70% 에탄올, 가장 구체적으로는 70% 에탄올을 사용할 수 있다. The extract of the dried vulgaris or semi-alcoholic malts is about 2 to 20 times the weight of the dried quince or semi-porous quince, specifically, about 3 to 5 times the volume of water, methanol, ethanol or butanol with a carbon number of 1 (C). A polar solvent of lower alcohols 1 ) to 4 (C 4 ) or a mixed solvent thereof can be used as an elution solvent. The elution solvent may be specifically 30 to 95% ethanol, 30 to 90% ethanol, or 30 to 80% ethanol, more specifically 30 to 70% ethanol, even more specifically 50 to 70% ethanol, Most specifically, 70% ethanol can be used.
상기 추출은 20 내지 100℃, 구체적으로 실온에서, 추출 기간은 약 4-12시간 내지 4-7일 동안 열수 추출, 냉침 추출, 환류 냉각 추출 또는 초음파 추출 등의 추출방법을 사용하여 추출할 수 있으나, 에스트로겐 수치 감소의 조절, 치료 활성이 있는 물질을 추출하는 방법이라면 제한 없이 이용될 수 있다. 구체적으로는, 1회 내지 5회 연속 추출하여 감압여과하고 그 여과추출물을 진공회전농축기로 20 내지 100℃, 더 구체적으로는 실온에서 감압 농축하여 물, 저급 알코올 또는 이들의 혼합용매에 가용한 불레기말 또는 반질불레기말 추출물을 수득한 결과물이 될 수 있으나, 본 발명의 에스트로겐 수치 감소의 조절, 치료 효과를 나타낼 수 있는 한, 이에 제한되지는 않고, 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 또는 이들의 조정제물 또는 정제물을 모두 포함한다. 상기 불레기말 또는 반질불레기말 추출물은 천연, 잡종, 변종식물로부터 추출될 수 있으며, 단독으로 또는 타 약학적 활성 추출물, 분획물 또는 화합물과의 결합뿐만 아니라 적당한 집합으로 사용될 수 있다. 본 발명의 실시예는 분말화된 불레기말 또는 반질불레기말에 에탄올을 넣고 추출 하였다 (실시예 1).The extraction may be performed at 20 to 100°C, specifically at room temperature, with an extraction period of about 4-12 hours to 4-7 days, using extraction methods such as hot water extraction, cold immersion extraction, reflux cooling extraction, or ultrasonic extraction. , any method for controlling the decrease in estrogen levels or extracting substances with therapeutic activity can be used without limitation. Specifically, extraction is performed 1 to 5 times in succession, filtered under reduced pressure, and the filtered extract is concentrated under reduced pressure using a vacuum rotary concentrator at 20 to 100°C, more specifically at room temperature, to produce a vulcanic substance soluble in water, lower alcohol, or a mixed solvent thereof. It may be the result of obtaining an extract of Gigi or Vanjilbulae Gigi, but is not limited thereto, as long as it can control and treat the effect of reducing estrogen levels according to the present invention, and may be obtained by drying the extract, diluted or concentrated extract of the extract, or dried extract. This includes all dried products obtained, or their crude or purified products. The extracts of the Malt officinalis or Banjil of the Extract can be extracted from natural, hybrid, or varietal plants, and can be used alone or in combination with other pharmaceutically active extracts, fractions, or compounds, as well as in appropriate combinations. In an example of the present invention, ethanol was added to powdered or semi-powdered bulaegi and extracted (Example 1).
상기 불레기말 또는 반질불레기말 추출물은 농도가 10 내지 80 μg/ml, 구체적으로 10 내지 60μg/ml, 더욱 구체적으로, 10μg/ml, 30μg/ml 또는 60μg/ml 일 수 있다. 상기 범위외의 추출물 농도에서는 목적하는 효과를 달성할 수 없다.The concentration of the extract of the Malt vulgaris or the Malt vulgaris may be 10 to 80 μg/ml, specifically 10 to 60 μg/ml, and more specifically, 10 μg/ml, 30 μg/ml, or 60 μg/ml. At extract concentrations outside the above range, the desired effect cannot be achieved.
본 발명의 조성물은 불레기말 추출물, 반질불레기말 추출물 또는 이의 혼합물을 포함할 수 있다. 상기 조성물은 구체적으로 반질불레기말 추출물을 포함할 수 있다. 본 발명의 일 실시예에서 반질불레기말 추출물이 미역, 다시마 등의 다른 갈조류보다 약 5배 이상의 우수한 에스트로겐 활성 효과를 갖는 것을 확인하였다. 특히 반질불레기말 추출물은 동일한 에탄올 농도로 추출된 불레기말 추출물에 비해서도 에스트로겐 수치 개선 효과가 우수한 것을 확인하였다 (도 8 참고).The composition of the present invention may include extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract or mixtures thereof. The composition may specifically include Banjilbulregi mal extract. In one embodiment of the present invention, it was confirmed that the extract of Banjilbulregi malae has an estrogen activity effect that is about 5 times more excellent than that of other brown algae such as seaweed and kelp. In particular, it was confirmed that the Banjilbulgi extract had an excellent effect in improving estrogen levels even compared to the Banjilbulgi extract extracted with the same ethanol concentration (see Figure 8).
본 발명에서 용어, "예방”은 상기 조성물의 투여에 의해 에스트로겐 수치 변화를 조절하거나 발병을 지연시키는 모든 행위를 의미하며, "치료"는 상기 조성물의 투여에 의해 에스트로겐 수치 변화에 의한 증상이 호전되거나 이롭게 변경되는 모든 행위를 의미한다. In the present invention, the term "prevention" refers to all actions that control changes in estrogen levels or delay the onset of changes by administering the composition, and "treatment" refers to improving symptoms caused by changes in estrogen levels by administering the composition. It refers to all actions that are beneficially changed.
본 발명에서 용어 '에스트로겐 수치 감소'는 에스트로겐 수치 감소와 관련된 질환을 의미하고, 구체적으로 폐경기로 인한 증상 또는 질환, 자궁내막증, 자궁 섬유종, 자궁평활근육종, 자궁내막암, 자궁암, 난소암, 유방암, 자궁출혈, 질 출혈, 월경과다, 자궁경부 상피내 종양, 샘근육증 및 갱년기 대사성 골질환 중 어느 하나 이상을 의미할 수 있고, 더욱 구체적으로, 갱년기 대사성 골질환을 의미할 수 있다. In the present invention, the term 'estrogen level reduction' refers to a disease related to a decrease in estrogen level, and specifically refers to symptoms or diseases caused by menopause, endometriosis, uterine fibroids, uterine leiomyosarcoma, endometrial cancer, uterine cancer, ovarian cancer, breast cancer, It may mean any one or more of uterine bleeding, vaginal bleeding, menorrhagia, cervical intraepithelial neoplasia, adenomyosis, and menopausal metabolic bone disease. More specifically, it may mean menopausal metabolic bone disease.
또한, 본 발명의 조성물은 에스트로겐 수치 감소와 관련된 여성 갱년기 증상 또는 질환을 개선, 예방 또는 치료하기 위한 용도로 사용할 수 있다. 상기 여성 갱년기 증상 또는 질환은 구체적으로 에스트로겐 분비 저하에 따른 질환일 수 있으며, 예를 들면 안면 홍조, 발한, 가슴 두근거림, 현기증, 이명, 고혈압, 비만, 고지혈증, 소화기 장애, 두통, 기억력 장애, 우울증, 피로감, 불안감, 신경과민, 수면 장애, 성욕 감퇴, 질 감염, 질 위축, 질 건조증, 질염, 방광염, 배뇨통, 급뇨, 피부 위축, 피부 건조, 근육통, 관절통, 및 골다공증으로 이루어진 군으로부터 선택된 하나 이상일 수 있다. Additionally, the composition of the present invention can be used to improve, prevent, or treat female menopausal symptoms or diseases related to decreased estrogen levels. The above female menopausal symptoms or diseases may specifically be diseases caused by decreased estrogen secretion, for example, facial flushing, sweating, heart palpitations, dizziness, tinnitus, high blood pressure, obesity, hyperlipidemia, digestive disorders, headaches, memory disorders, and depression. One or more days selected from the group consisting of fatigue, anxiety, nervousness, sleep disturbance, decreased libido, vaginal infection, vaginal atrophy, vaginal dryness, vaginitis, cystitis, pain during urination, urgent urination, skin atrophy, dry skin, muscle pain, joint pain, and osteoporosis. You can.
본 발명의 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는 약학적 조성물은 약학적 조성물의 제조에 통상적으로 사용하는 적절한 담체, 부형제 및 희석제를 더 포함할 수 있다. 구체적으로, 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 이때, 상기 조성물에 포함되는 반질불레기말 추출물의 함량은 특별히 이에 제한되지 않으나, 조성물 총 중량에 대하여 0.0001 내지 10 중량%로 포함될 수 있으며, 보다 구체적으로는, 0.001 내지 1 중량% 포함될 수 있다.The pharmaceutical composition containing the extract of the anthurium vulgaris or the anthurium vulgaris of the present invention as an active ingredient may further include appropriate carriers, excipients, and diluents commonly used in the preparation of pharmaceutical compositions. Specifically, it can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, external preparations, suppositories, and sterile injectable solutions. At this time, the content of the Banjilbulregi extract included in the composition is not particularly limited, but may be included in 0.0001 to 10% by weight, more specifically, 0.001 to 1% by weight, based on the total weight of the composition.
상기 약학적 조성물은 담체, 부형제 및 희석제로는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로스, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 포함할 수 있다. 상기 조성물을 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함될 수 있으며, 이러한 고형제제는 반질불레기말 추출물을 포함하여 한가지 이상의 부형제 예를 들면, 전분, 칼슘카보네이트(calcium carbonate), 수크로스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제될 수 있다. 또한 단순한 부형제 이외에 마그네슘 스티레이트, 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당될 수 있는데, 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구 투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함될 수 있다. 비수성용제, 현탁제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.The pharmaceutical composition includes carriers, excipients, and diluents such as lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, and methyl. It may contain cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulating the composition, it can be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration may include tablets, pills, powders, granules, capsules, etc., and these solid preparations may contain one or more excipients, such as starch, calcium carbonate, It can be prepared by mixing sucrose, lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium styrate and talc are also used. Liquid preparations for oral use may include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives are included. may be included. Preparations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurin, glycerogeratin, etc. can be used.
본 발명의 상기 조성물은 약학적으로 유효한 양으로 투여될 수 있다. The composition of the present invention can be administered in a pharmaceutically effective amount.
본 발명에서 용어, "약학적으로 유효한 양"은 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분한 양을 의미하며, 유효 용량 수준은 개체 종류 및 중증도, 연령, 성별, 질환의 종류, 약물의 활성, 약물에 대한 민감도, 투여 시간, 투여 경로 및 배출 비율, 치료기간, 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다. 본 발명의 조성물은 개별 치료제로 투여하거나 다른 치료제와 병용하여 투여될 수 있고 종래의 치료제와는 순차적 또는 동시에 투여될 수 있다. 그리고 단일 또는 다중 투여될 수 있다. 상기 요소를 모두 고려하여 부작용 없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하며, 당업자에 의해 용이하게 결정될 수 있다. 본 발명의 조성물의 바람직한 투여량은 환자의 상태 및 체중, 질병의 정도, 약물 형태, 투여경로 및 기간에 따라 다르지만, 바람직한 효과를 위해서, 본 발명의 불레기말 또는 반질불레기말 추출물은 1일 0.0001 내지 1000 mg/kg으로, 바람직하게는 0.001 내지 1000 mg/kg으로 투여될 수 있고, 보다 구체적으로는, 30 내지 120 mg/kg, 더욱 구체적으로는 50 내지 100mg/kg, 가장 구체적으로는 50 또는 100mg/kg 이 투여될 수 있다. 투여는 하루에 한번 투여할 수도 있고, 수회 나누어 투여할 수도 있다. 상기 조성물은 쥐, 가축, 인간 등의 다양한 포유동물에 다양한 경로로 투여할 수 있으며, 투여의 방식은 당업계의 통상적인 방법이라면 제한없이 포함하며, 예를 들어, 경구, 직장 또는 정맥, 근육, 피하, 자궁내 경막 또는 뇌혈관 내 주사에 의해 투여될 수 있다. 본 발명의 약제학적 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사는 소망하는 치료 또는 예방에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.In the present invention, the term "pharmaceutically effective amount" refers to an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type and severity of the individual, age, gender, and condition of the disease. It can be determined based on factors including the type, activity of the drug, sensitivity to the drug, time of administration, route of administration and excretion rate, duration of treatment, drugs used simultaneously, and other factors well known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And it can be administered single or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve maximum effect with the minimum amount without side effects, and can be easily determined by a person skilled in the art. The preferred dosage of the composition of the present invention varies depending on the patient's condition and weight, degree of disease, drug form, administration route, and period, but for desirable effects, the extract of the Malt vulgaris or Banjyl vulgaris extract of the present invention should be administered in an amount of 0.0001 to 0.0001 per day. It can be administered at 1000 mg/kg, preferably at 0.001 to 1000 mg/kg, more specifically at 30 to 120 mg/kg, more specifically at 50 to 100 mg/kg, most specifically at 50 or 100 mg. /kg can be administered. Administration may be administered once a day, or may be administered several times. The composition can be administered to various mammals such as rats, livestock, and humans by various routes, and the method of administration includes without limitation any method conventional in the art, for example, orally, rectally, intravenously, intramuscularly, or intravenously. It may be administered by subcutaneous, intrauterine, intrathecal, or intracerebrovascular injection. The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity. Usually, a skilled physician can easily determine and prescribe an effective dosage for the desired treatment or prevention.
본 발명의 약학적 조성물은 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화 함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때 제형은 오일 또는 수성 매질중의 용액, 현탁액 또는 유화액 형태이거나 엑스제, 분말제, 과립제, 정제 또는 캅셀제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수 있다.The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily performed by a person skilled in the art. Alternatively, it can be manufactured by placing it in a multi-capacity container. At this time, the formulation may be in the form of a solution, suspension, or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet, or capsule, and may additionally contain a dispersant or stabilizer.
본 발명의 구체적인 일 실시예에서는, 불레기말 또는 반질불레기말 추출물의 독성 및 에스트로겐 활성 변화능 (도 1)이 있음을 확인하였다. In a specific example of the present invention, it was confirmed that the extract of the horse vulgaris or banjil vulgaris has the ability to change toxicity and estrogen activity (Figure 1).
본 발명의 또 다른 하나의 양태는 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감소의 조절, 예방 또는 개선용 식품 조성물을 제공한다. Another aspect of the present invention provides a food composition for controlling, preventing, or improving a decrease in estrogen levels, comprising an extract of the end of the day or the end of the day as an active ingredient.
구체적으로, 본 발명에 따른 식품 조성물은 상기 약제학적 조성물과 동일한 방식으로 제제화되어 건강기능식품으로 이용하거나, 각종 식품에 첨가할 수 있다. Specifically, the food composition according to the present invention can be formulated in the same way as the pharmaceutical composition and used as a health functional food or added to various foods.
본 발명의 추출물을 식품 첨가물로 사용할 경우, 상기 불레기말 또는 반질불레기말 추출물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용할 수 있고, 통상의 방법에 따라 적절하게 사용할 수 있다. 유효 성분의 혼합양은 사용 목적(예방, 건강 또는 치료적 처치)에 따라 적합하게 결정될 수 있으며, 식품학적으로 허용 가능한 식품 보조 첨가제를 추가로 포함할 수 있다. 본 발명의 조성물은 천연물로부터 유래한 추출물을 유효성분으로 하므로 안정성 면에서 문제가 없기 때문에 혼합량에 큰 제한은 없다. 또한, 본 발명의 식품 조성물은 통상적인 의미의 식품을 모두 포함할 수 있으며, 기능성 식품, 건강기능식품 등 당업계에 알려진 용어와 혼용 가능하다.When using the extract of the present invention as a food additive, the above-mentioned extract of Bolegifolia or Banjilberchia can be added as is or used together with other foods or food ingredients, and can be used appropriately according to conventional methods. The amount of active ingredients mixed can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment), and foodologically acceptable food supplements may be additionally included. Since the composition of the present invention uses extracts derived from natural products as an active ingredient, there is no problem in terms of stability, so there is no significant limitation on the mixing amount. In addition, the food composition of the present invention can include all foods in the conventional sense, and can be used interchangeably with terms known in the art such as functional food and health functional food.
본 발명의 용어, "기능성 식품"은 건강기능식품에 관한 법률 제6727호에 따른 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미하며, "기능성"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻을 목적으로 섭취하는 것을 의미한다. 또한, "건강기능식품"은 건강보조의 목적으로 특정성분을 원료로 하거나 식품 원료에 들어있는 특정성분을 추출, 농축, 정제, 혼합 등의 방법으로 제조, 가공한 식품을 말하며, 상기 성분에 의해 생체방어, 생체리듬의 조절, 질병의 방지와 회복 등 생체조절기능을 생체에 대하여 충분히 발휘할 수 있도록 설계되고 가공된 식품을 말하는 것으로서, 상기 건강식품용 조성물은 질병의 예방 및 질병의 회복 등과 관련된 기능을 수행할 수 있다.The term "functional food" in the present invention refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and "functional" refers to food that is useful for the human body. It means ingestion for the purpose of controlling nutrients for structure and function or obtaining useful health effects such as physiological effects. In addition, “health functional food” refers to a food manufactured or processed using specific ingredients as raw materials or by extracting, concentrating, refining, or mixing specific ingredients contained in food ingredients for the purpose of health supplementation. It refers to food designed and processed to fully exert bioregulatory functions on the living body, such as biodefense, regulation of biorhythm, prevention and recovery of disease, etc. The composition for health food has functions related to prevention of disease and recovery from disease. can be performed.
본 발명의 조성물이 사용될 수 있는 식품의 종류에는 제한이 없다. 아울러 본 발명의 불레기말 추출물 또는 반질불레기말 추출물을 활성성분으로 포함하는 조성물은 당업자의 선택에 따라 식품에 함유될 수 있는 적절한 기타 보조 성분과 공지의 첨가제를 혼합하여 제조할 수 있다. 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 쵸코렛, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림 류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 본 발명에 따른 추출물을 주성분으로 하여 제조한 즙, 차, 젤리 및 주스 등에 첨가하여 제조할 수 있다.There are no restrictions on the types of food in which the composition of the present invention can be used. In addition, a composition containing the extract of the vulgaris root of the present invention or the root of the vulgaris extract of the present invention as an active ingredient can be prepared by mixing known additives with other appropriate auxiliary ingredients that may be contained in foods according to the selection of a person skilled in the art. Examples of foods that can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes, etc., and they can be manufactured by adding them to juices, teas, jellies, juices, etc. prepared using the extract according to the present invention as a main ingredient.
또한, 본 발명에 적용될 수 있는 식품에는 예컨대, 특수영양식품 (예: 조제유류, 영,유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예: 라면류, 국수류 등), 건강보조식품, 조미식품(예: 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예:스낵류), 유가공품(예: 발효유, 치즈 등), 기타 가공식품, 김치, 절임식품(각종 김치류, 장아찌 등), 음료(예: 과실, 채소류 음료, 두유류, 발효음료류 등), 천연조미료(예, 라면스프 등) 등 모든 식품을 포함할 수 있다.In addition, foods that can be applied to the present invention include, for example, special nutritional foods (e.g., infant formula, infant and toddler food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g., ramen, noodles, etc.), and health supplements. , seasoned foods (e.g. soy sauce, soybean paste, red pepper paste, mixed paste, etc.), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.) ), beverages (e.g. fruit, vegetable drinks, soy milk, fermented beverages, etc.), natural seasonings (e.g. ramen soup, etc.), etc.
본 발명의 건강기능식품 조성물이 음료의 형태로 사용될 경우에는 통상의 음료와 같이 여러 가지 감미제, 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상기 외에 본 발명의 건강기능식품 조성물은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그밖에 천연 과일쥬스, 과일쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다.When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweeteners, flavoring agents, or natural carbohydrates as additional ingredients like ordinary beverages. In addition to the above, the health functional food composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, and glycerin. , alcohol, and carbonating agents used in carbonated beverages. In addition, it may contain pulp for the production of natural fruit juice, fruit juice drinks, and vegetable drinks.
본 발명의 다른 하나의 양태는 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감소의 조절, 예방 또는 개선용 의약외품 조성물을 제공한다.Another aspect of the present invention provides a quasi-drug composition for controlling, preventing, or improving a decrease in estrogen levels, comprising an extract of the end of the day or the end of the day as an active ingredient.
본 발명에서 용어, "의약외품"은 사람이나 동물의 질병을 치료, 경감, 처치 또는 예방할 목적으로 사용되는 섬유, 고무제품 또는 이와 유사한 것, 인체에 대한 작용이 약하거나 인체에 직접 작용하지 아니며, 기구 또는 기계가 아닌 것과 이와 유사한 것, 감염 예방을 위하여 살균, 살충 및 이와 유사한 용도로 사용되는 제제 중 하나에 해당하는 물품으로서, 사람이나 동물의 질병을 진단, 치료, 경감, 처치 또는 예방할 목적으로 사용하는 물품 중 기구, 기계 또는 장치가 아닌 것 및 사람이나 동물의 구조와 기능에 약리학적 영향을 줄 목적으로 사용하는 물품 중 기구, 기계 또는 장치가 아닌 것을 제외한 물품을 의미하며, 피부 외용제 및 개인위생용품도 포함한다.In the present invention, the term "quasi-drug" refers to fibers, rubber products, or similar products used for the purpose of treating, alleviating, treating, or preventing diseases in humans or animals, or devices that have a weak effect on the human body or do not directly act on the human body, or devices. Or, it is an article that is not a machine or similar, or an agent used for sterilization, insecticide, and similar purposes to prevent infection, and is used for the purpose of diagnosing, treating, alleviating, treating, or preventing diseases in humans or animals. It refers to articles other than instruments, machines, or devices among articles used for the purpose of having a pharmacological effect on the structure and function of humans or animals, excluding articles for external use on the skin and personal hygiene. Also includes supplies.
본 발명의 불레기말 또는 반질불레기말 추출물을 유효성분으로 포함하는, 에스트로겐 수치 감소의 조절, 예방 또는 개선을 목적으로 의약외품 조성물에 첨가할 경우, 상기 추출물을 그대로 첨가하거나 다른 분획물이나 다른 의약외품 성분과 함께 사용할 수 있고, 통상적인 방법에 따라 적절하게 사용할 수 있다. 유효 성분의 혼합량은 사용 목적에 따라 적합하게 결정할 수 있다.When added to a quasi-drug composition for the purpose of controlling, preventing, or improving the decrease in estrogen levels, which contains the extract of the end of the perennial or half-drug of the present invention as an active ingredient, the extract may be added as is or together with other fractions or other quasi-drug ingredients. It can be used, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.
상기 피부외용제는 특별히 이에 제한되지 않으나, 바람직하게는 연고제, 로션제, 스프레이제, 패취제, 크림제, 산제, 현탁제, 겔제 또는 젤의 형태로 제조되어 사용될 수 있다. 상기 개인위생용품에는 특별히 이에 제한되지 않으나, 바람직하게는 비누, 화장품, 물티슈, 휴지, 샴푸, 피부 크림, 얼굴 크림, 치약, 립스틱, 향수, 메이크업, 파운데이션, 볼터치, 마스카라, 아이섀도우, 선스크린 로션, 모발 손질 제품, 에어프레쉬너 겔 또는 세정 겔일 수 있다. 또한, 본 발명의 의약외품 조성물의 또 다른 예로 소독청결제, 샤워폼, 가그린, 물티슈, 세제비누, 핸드워시, 가습기 충진제, 마스크, 연고제 또는 필터충진제가 있다.The skin external preparation is not particularly limited thereto, but is preferably prepared and used in the form of ointment, lotion, spray, patch, cream, powder, suspension, gel or gel. The personal hygiene products are not particularly limited, but are preferably soap, cosmetics, wet tissues, toilet paper, shampoo, skin cream, face cream, toothpaste, lipstick, perfume, makeup, foundation, blush, mascara, eye shadow, and sunscreen. It may be a lotion, hair care product, air freshener gel, or cleaning gel. Additionally, other examples of the quasi-drug composition of the present invention include disinfectant cleaners, shower foams, garnishes, wet tissues, detergent soaps, hand washes, humidifier fillers, masks, ointments, or filter fillers.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.
실시예 1: 불레기말 또는 반질불레기말 추출물 제조Example 1: Preparation of extract from the end of the vulgaris or the end of the vultures
불레기말 또는 반질불레기말은 물로 깨끗이 세척하여 그늘에서 건조한 후, 와링 브랜드로 분말화 시켰다. 분말화된 불레기말 또는 반질불레기말 500g을 에탄올 5ℓ에 넣고 추출기를 통해 5-10시간 동안 70℃로 추출한 후, 여지(와트만사, 미국)로 감압 여과한 다음, 여과 추출물은 진공회전농축기로 실온에서 에탄올 용매를 제거한 후 추출된 잔사로서 불레기말 또는 반질불레기말 추출물을 수득하였다. Buleggi Mal or half-jilbulgi Mal was washed thoroughly with water, dried in the shade, and then powdered with Waring brand. Add 500 g of powdered or semi-powdered dried ginseng into 5 liters of ethanol, extract at 70°C for 5-10 hours through an extractor, filter under reduced pressure with filter paper (Whatman, USA), and then extract the filtered extract at room temperature using a vacuum rotary concentrator. After removing the ethanol solvent, the extracted residue was obtained as an extract of vulgaris mal or banjil vulgaris mal.
실험예 1: 불레기말 또는 반질불레기말 에탄올 함량 변화에 따른 추출물의 세포독성 및 에스트로겐 활성 확인Experimental Example 1: Confirmation of cytotoxicity and estrogen activity of the extract according to the change in ethanol content of Bolegioma or Banjilbeogi.
독성 확인을 위해 T47D-KBluc세포를 96 well plate에 3Х104 cells/well 로 seeding 후 overnight 하였다. 그 후, 24시간 동안 추출물 처리하고 MTT 시약과 3시간 동안 반응 시킨 후 상등액을 제거하고, 생성된 염을 DMSO에 녹여 흡광도를 측정하였다. 실시예 1의 불레기말 또는 반질불레기말 추출물의 에스트로겐 활성 평가를 위해 T47D-KBluc 세포주를 이용하였다. 안정화 시킨 세포는 seeding 전 assay media로 교체 후 24시간 인큐베이터에서 배양하고 96 well plate에 3x104 cells/well로 seeding하여 overnight한다. 이 후 불레기말 또는 반질불레기말 추출물을 처리 후 다시 24시간 인큐베이터에서 배양하고 상등액을 제거하여 1X lysis buffer 60 μl/well로 30분 lysis 시키고 96 well white plate에 cell lysate 25 μl 처리 후 luminescent microplate reader로 검출한다. 이 때 조건은 substrat volume이 25 μl, duration이 1s, counting time은 2s로 설정한다.To confirm toxicity, T47D-KBluc cells were seeded at 3Х10 4 cells/well in a 96 well plate and left overnight. Afterwards, the extract was treated for 24 hours and reacted with MTT reagent for 3 hours, the supernatant was removed, the resulting salt was dissolved in DMSO, and the absorbance was measured. The T47D-KBluc cell line was used to evaluate the estrogen activity of the extract from Example 1. The stabilized cells were replaced with assay media before seeding, cultured in an incubator for 24 hours, and seeded at 3x10 4 cells/well in a 96 well plate overnight. Afterwards, the cells were treated with the extract of the lysicum or seminiferous root, cultured in the incubator for another 24 hours, removed the supernatant, lysed for 30 minutes with 60 μl/well of 1X lysis buffer, and treated with 25 μl of cell lysate on a 96 well white plate using a luminescent microplate reader. detect. At this time, the conditions are set to substrat volume of 25 μl, duration of 1 s, and counting time of 2 s.
그 결과, 도 1에서 확인되는 바와 같이 모든 에탄올 함량에서 불레기말 또는 반질불레기말 추출물의 독성은 나타나지 않았다 (도 1 (A)). 다만, 70% 이상 농도의 에탄올을 이용하여 추출된 불레기말 추출물 또는 반질 불레기말 추출물에서 에스트로겐 활성 변화가 큰 것을 확인하였다 (도 1 (B)).As a result, as confirmed in Figure 1, no toxicity was observed in the extracts of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the extract of the ethanol content at any ethanol content (Fig. 1 (A)). However, it was confirmed that there was a large change in estrogen activity in the extract or half-length vulgaris extract extracted using ethanol at a concentration of 70% or higher (Figure 1 (B)).
실험예 2: 불레기말 추출물에 의한 에스트로겐 신호전달 분자의 인산화 조절 효과 확인Experimental Example 2: Confirmation of the effect of phosphorylation regulation of estrogen signaling molecules by Extract of the vulgaris root
신호전달분자의 인산화를 측정하기 위하여 MCF-7 세포와 T47D 세포를 6 well plate에 1 Х106 cells/well 로 seeding 후 안정화 하고, serum free 배지로 교환하여 12시간 배양하였다. 그 후, 추출물을 15, 30분 처리 하고 harvest하였다. 얻어진 세포에서 단백질을 추출 및 정량하고, Western blot을 통해 Akt와 Erk의 인산화를 확인하였다. To measure the phosphorylation of signaling molecules, MCF-7 cells and T47D cells were seeded in a 6-well plate at 1 Х10 6 cells/well, stabilized, replaced with serum free medium, and cultured for 12 hours. Afterwards, the extract was treated for 15 and 30 minutes and harvested. Proteins were extracted and quantified from the obtained cells, and phosphorylation of Akt and Erk was confirmed through Western blot.
그 결과, MCF-7 (도 2(A)) 및 T47D 세포 (도 2(B))에서 불레기말 추출물의 Akt 와 Erk 분자의 인산화를 확인하였다. 불레기말 추출물 처리에 의해 Akt의 인산화가 증가를 확인하였다. 하지만 Erk의 인산화는 대조군 대비 저농도에서 감소하지만 고농도군에서 Erk의 인산화가 증가하는 경향을 확인하였다.As a result, phosphorylation of Akt and Erk molecules in the vulgaris extract was confirmed in MCF-7 (Figure 2(A)) and T47D cells (Figure 2(B)). It was confirmed that the phosphorylation of Akt was increased by treatment with the vulgaris extract. However, the phosphorylation of Erk decreased at low concentration compared to the control group, but it was confirmed that the phosphorylation of Erk tended to increase in the high concentration group.
실험예 3: 반질불레기말 추출물에 의한 동물모델에서의 체온 변화Experimental Example 3: Changes in body temperature in animal models caused by Banjilbulregi horse extract
반질불레기말 추출물의 갱년기 증상 완화 효과를 평가하기 위해서 C57BL/6 마우스를 이용한 난소 적출 모델을 사용하였다. 난소 적출 모델은 난소에서 분비되는 호르몬이 없기 때문에 갱년기를 유도 할 수 있다. 6주령의 C57BL/6 마우스를 난소 적출 후 1주 동안 회복기를 가지고, 그 후 6주 동안 갱년기를 유도하였다. To evaluate the effect of Banjilbulregi mal extract on alleviating menopausal symptoms, an ovariectomy model using C57BL/6 mice was used. The ovariectomy model can induce menopause because there are no hormones secreted by the ovaries. Six-week-old C57BL/6 mice had a recovery period for one week after ovariectomy, and then menopause was induced for six weeks.
갱년기가 유도된 마우스에 6주 동안 반질불레기말 에탄올 추출물과 에스트라디올을 매일 경구 투여 하였고 매주 체온을 측정하였다. Mice in which menopause was induced were orally administered ethanol extract of the quinceafolium and estradiol every day for 6 weeks, and their body temperature was measured every week.
그 결과 동물모델에서 하복부의 체온 측정시 추출물 처리군에서 OVX군 대비 전체적으로 체온이 높게 나타났다 (도 3). 이는 OVX군 대비 대사량이 증가하는 것을 의미한다. As a result, when measuring the body temperature of the lower abdomen in the animal model, the overall body temperature was found to be higher in the extract-treated group compared to the OVX group (Figure 3). This means that the metabolic rate increases compared to the OVX group.
실험예 4: 반질불레기말 추출물의 골 대사 관련 이미지 분석 Experimental Example 4: Image analysis related to bone metabolism of Banjilbulregi extract
실험예 3에서 준비된 갱년기 모델 마우스의 대퇴골을 적출하였다. 적출된 대퇴골을 10% formalin에 24시간 고정하고 PBS로 채워진 polystyrene tube에 넣어 micro CT 분석을 하였다. 분석 조건은 다음과 같다. 축의 회전은 0.42° 씩 360.36°까지 측정하였고, 0.5mm aluminum filter를 사용하였다. 전압 및 전류는 은 88 kV, 112 μA를 사용하여 9 μm 해상도의 이미지를 얻었다 (도 4(A)). 그 결과 난소적출에 의해 골 내부가 비어있는 상태에 비하여 반질불레기말 추출물 처리를 통해 내부 골이 차오르는 현상을 확인하였다. The femur of the menopausal model mouse prepared in Experimental Example 3 was removed. The extracted femur was fixed in 10% formalin for 24 hours and placed in a polystyrene tube filled with PBS for micro CT analysis. The analysis conditions are as follows. Axial rotation was measured up to 360.36° in 0.42° increments, and a 0.5mm aluminum filter was used. The voltage and current were 88 kV and 112 μA to obtain an image with 9 μm resolution (Figure 4(A)). As a result, compared to the state where the inside of the bone was empty due to ovariectomy, the phenomenon of internal bone filling was confirmed through treatment with Banjilbulregi mal extract.
또한 얻어진 이미지를 분석프로그램을 통하여 대퇴골의 성장판 아래 0.5mm에서 2.5mm 사이를 분석하여 bone volume/total volume (BV/TV) (도 4(B)), trabecular thickness (Tb.Th) (도 4(C)), trabecular number (Tb.N) (도 4(D)) 그리고 trabecular bone mineral density (BMD) (도 4(E)) 의 수치를 확인하였다. 그 결과 반질불레기말 추출물 처리시 두께, 함량 및 밀도 등이 증가하는 활성을 확인하였다.In addition, the obtained image was analyzed between 0.5mm and 2.5mm below the growth plate of the femur through an analysis program to determine bone volume/total volume (BV/TV) (Figure 4(B)) and trabecular thickness (Tb.Th) (Figure 4(B)). C)), trabecular number (Tb.N) (Figure 4(D)), and trabecular bone mineral density (BMD) (Figure 4(E)) were confirmed. As a result, it was confirmed that the thickness, content, and density increased when treated with the Banjilbulregi extract.
실험예 5: 반질불레기말 추출물의 난소 적출 여성 갱년기 동물모델의 혈청 분석을 골대사 바이오마커 관련 완화 효과 확인Experimental Example 5: Confirmation of the alleviating effect related to bone metabolism biomarkers through serum analysis of ovariectomized female menopausal animal model of Banjilbulregi horse extract
실험예 3에서 준비된 갱년기 모델 마우스의 심장 채혈하였고, 채혈된 혈액을 SST (serum separate tube)에 처리한 후, 5000 RPM으로 5분간 원심 분리하여 혈청을 얻었다. 혈청 내의 CTX (c-terminal telopeptide) 골 흡수 마커와 ALP (alkaline phosphatase) 골 형성 마커를 면역측정법을 이용하여 측정하였다. 이는 각각의 특정 마커를 인식하는 항체가 코팅된 plate에 시료에 존재하는 마커 분자들이 결합하게 되고, 여기에 HRP (horseradish peroxidase)와 결합한 항체가 plate 내에 남아있는 마커분자에 결합하여 측정하는 방법으로 실험하였다.Blood was collected from the heart of the menopausal model mouse prepared in Experimental Example 3, the collected blood was processed in a SST (serum separate tube), and then centrifuged at 5000 RPM for 5 minutes to obtain serum. CTX (c-terminal telopeptide) bone resorption marker and ALP (alkaline phosphatase) bone formation marker in serum were measured using immunoassay. This is an experiment in which the marker molecules present in the sample bind to a plate coated with an antibody that recognizes each specific marker, and the antibody bound to HRP (horseradish peroxidase) binds to the marker molecules remaining in the plate and measures them. did.
RANKL (도 5(A)), osteoprotegerin (OPG) (도 5(B)), RANKL/OPG 비율 (도 5(C)) 의 ELISA 분석 결과 골형성과 관련된 주요 바이오마커 분석 단계에서 반질불레기말 추출물 처리시 증가 및 감소를 통한 조절 효과를 확인하였다.Results of ELISA analysis of RANKL (FIG. 5(A)), osteoprotegerin (OPG) (FIG. 5(B)), and RANKL/OPG ratio (FIG. 5(C)). In the analysis step of major biomarkers related to bone formation, the extract The control effect was confirmed through increase and decrease during treatment.
실험예 6: 반질불레기말 추출물의 난소 적출 여성 갱년기 동물모델의 혈청 분석을 골대사 바이오마커 관련 완화 효과 및 호르몬 변화 확인Experimental Example 6: Confirmation of alleviating effect and hormonal changes related to bone metabolism biomarkers through serum analysis of ovariectomized female menopausal animal model of Banjilbulregi horse extract
실험예 5와 동일한 방법으로 혈청분석을 진행하였다. Serum analysis was performed in the same manner as in Experimental Example 5.
그 결과, 도 6 (A), (B)에서 확인되는 바와 같이 난소를 적출한 마우스의 경우 난소를 적출하지 않은 마우스와 비교하여 혈청 내 ALP와 CTX의 수치가 증가하는 것을 확인하였다. 이는 골다공증에서 나타나는 증상으로 증가된 bone turn over에 의해 골의 미세구조가 퇴화하게 된다. 난소를 적출한 마우스에 반질불레기말 추출물을 처리한 경우 처리하지 않은 마우스와 비교했을 때, ALP와 CTX의 수치가 감소하는 것을 확인하였다. 특히 고농도(100 mg/kg-인체적용시 용량 500 mg 미만)에서 비교군인 에스트라디올 활성과 유사한 효과를 나타내는 것을 확인하였다. As a result, as shown in Figures 6 (A) and (B), it was confirmed that the levels of ALP and CTX in the serum of mice whose ovaries were removed were increased compared to mice whose ovaries were not removed. This is a symptom of osteoporosis and the microstructure of the bone deteriorates due to increased bone turn over. When ovariectomized mice were treated with Banjilbulregi mal extract, the levels of ALP and CTX were confirmed to decrease compared to untreated mice. In particular, it was confirmed that at high concentrations (100 mg/kg - less than 500 mg when applied to the human body), it exhibited a similar effect to the activity of estradiol, a comparison group.
또한, follicle stimulating horone (FSH)의 ELISA 분석 결과 반질불레기말 추출물 처리에 의해 난소 적출 군 대비 감소하는 것을 확인하였다 (도 6 (C)).In addition, as a result of ELISA analysis of follicle stimulating hormone (FSH), it was confirmed that the follicle stimulating hormone (FSH) decreased compared to the ovariectomized group by treatment with the Banjilbulregi mal extract (Figure 6 (C)).
실험예 7: 반질불레기말 추출물의 난소 적출 여성 갱년기 동물모델에서 신경전달물질 바이오마커 관련 개선(증가) 효과 확인Experimental Example 7: Confirmation of the improvement (increase) effect of Banjilbulregi horse extract related to neurotransmitter biomarkers in an ovariectomized female menopausal animal model
실험예 3에서 준비된 갱년기 모델 마우스에서 적출한 뇌를 Lysis buffer에서 homogenizer를 이용하여 분쇄 후, 12000 RPM으로 10분간 원심 분리하여 마우스 brain lysate를 얻었고, 이를 정량하여 단백질 농도를 확인하였다. 그리고 brain lystate의 뇌 내의 신경전달물질인 norepinephrine과 serotonin의 농도를 면역측정법을 이용하여 측정한 후 사전에 얻은 마우스 brain lysate의 단백질 농도로 표준화 하였다. The brain extracted from the menopausal model mouse prepared in Experimental Example 3 was pulverized in lysis buffer using a homogenizer and then centrifuged at 12000 RPM for 10 minutes to obtain mouse brain lysate, which was quantified to confirm protein concentration. In addition, the concentrations of norepinephrine and serotonin, which are neurotransmitters in the brain lystate, were measured using an immunoassay and then normalized to the protein concentration of the previously obtained mouse brain lysate.
그 결과, 난소를 적출한 마우스의 경우 난소를 적출하지 않은 마우스와 비교하여 혈청 내 norepinephrine (도 7 (A)) 과 serotonin (도 7 (B)) 의 수치가 감소하는 것을 확인하였다. 그리고 난소를 적출한 마우스에 반질불레기말 추출물을 처리한 경우 처리하지 않은 마우스와 비교했을 때, norepinephrine과 serotonin의 수치가 증가하는 것을 확인하였다.As a result, it was confirmed that the levels of norepinephrine (Figure 7(A)) and serotonin (Figure 7(B)) in the serum of mice whose ovaries were removed were decreased compared to mice whose ovaries were not removed. And when mice with ovaries removed were treated with Banjilbulregi mal extract, the levels of norepinephrine and serotonin were confirmed to increase compared to untreated mice.
실험예 8: 다른 갈조류와의 활성 비교Experimental Example 8: Comparison of activity with other brown algae
실험예 1의 에스트로겐 활성 측정과 동일한 방법으로 진행하였다.The same method as the estrogen activity measurement in Experimental Example 1 was performed.
그 결과, 도 8에서 확인되는 바와 같이 본 연구에서 사용한 반질불레기말과 불레기말과 다른 갈조류 중 다시마, 미역과의 에스트로겐 비교활성 실험을 진행하였을 때 불레기말과 반질불레기말이 타 소재에 비해 에스트로겐 활성이 농도의존적으로 매우 우수함을 확인하였고, 특히 반질불레기말은 불레기말에 비해서도 우수한 에스트로겐 활성을 갖는 것을 확인하였다. As a result, as can be seen in Figure 8, when a comparative estrogen activity test was conducted with kelp and seaweed among other brown algae and the brown algae used in this study, the estrogen and half-colored algae showed estrogen activity compared to other materials. It was confirmed that this was very good in a concentration-dependent manner, and in particular, it was confirmed that Banjilbulegimal had superior estrogen activity compared to Buleggimal.
상기와 같은 결과를 종합하면, 본 발명의 반질불레기말 추출물은 에스트로겐 수치 감소, 가령 여성갱년기 증상,을 효과적으로 예방 또는 치료할 수 있음을 시사하는 것이다.Taken together, the above results suggest that the Banjilbuljeong extract of the present invention can effectively prevent or treat decreased estrogen levels, such as female menopausal symptoms.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (7)
상기 에스트로겐 수치 감소 관련 질환은 자궁내막증, 자궁 섬유종, 자궁평활근육종, 자궁내막암, 자궁암, 난소암, 유방암, 자궁출혈, 질 출혈, 월경과다, 자궁경부 상피내 종양, 샘근육증 또는 갱년기 대사성 골질환인 것인 약학적 조성물.
A pharmaceutical composition for preventing or treating diseases related to decreased estrogen levels, comprising extract of Banjilbullegicum as an active ingredient,
The diseases associated with decreased estrogen levels include endometriosis, uterine fibroids, uterine leiomyosarcoma, endometrial cancer, uterine cancer, ovarian cancer, breast cancer, uterine bleeding, vaginal bleeding, menorrhagia, cervical intraepithelial neoplasia, adenomyosis, or menopausal metabolic bone disease. A pharmaceutical composition.
The pharmaceutical composition according to claim 1, wherein the extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
The pharmaceutical composition according to claim 2, wherein the lower alcohol is methanol, ethanol, or butanol.
The pharmaceutical composition according to claim 1, wherein the extract is extracted with 30 to 95% ethanol.
The pharmaceutical composition according to claim 1, wherein the extract has a concentration of 10 to 80 μg/ml.
The pharmaceutical composition according to claim 1, wherein the dosage of the extract is 30 to 120 mg/kg.
상기 에스트로겐 수치 감소 관련 질환은 자궁내막증, 자궁 섬유종, 자궁평활근육종, 자궁내막암, 자궁암, 난소암, 유방암, 자궁출혈, 질 출혈, 월경과다, 자궁경부 상피내 종양, 샘근육증 또는 갱년기 대사성 골질환인 것인 식품 조성물.
A food composition for preventing or improving diseases related to decreased estrogen levels, comprising extract of Banjilbulregi horse as an active ingredient,
The diseases associated with decreased estrogen levels include endometriosis, uterine fibroids, uterine leiomyosarcoma, endometrial cancer, uterine cancer, ovarian cancer, breast cancer, uterine bleeding, vaginal bleeding, menorrhagia, cervical intraepithelial neoplasia, adenomyosis, or menopausal metabolic bone disease. A food composition that is.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020210066226A KR102588771B1 (en) | 2021-05-24 | 2021-05-24 | COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020210066226A KR102588771B1 (en) | 2021-05-24 | 2021-05-24 | COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20220158430A KR20220158430A (en) | 2022-12-01 |
KR102588771B1 true KR102588771B1 (en) | 2023-10-13 |
Family
ID=84440588
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020210066226A KR102588771B1 (en) | 2021-05-24 | 2021-05-24 | COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR102588771B1 (en) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101260696B1 (en) | 2011-01-03 | 2013-05-13 | 한국식품연구원 | Composition for invigorating GABAA-benzodiazepine receptor and composition having anxiolitic, anti-convulsant, anti-depressant and sleep-improving effect containing Brown Seaweed extract |
-
2021
- 2021-05-24 KR KR1020210066226A patent/KR102588771B1/en active IP Right Grant
Non-Patent Citations (2)
Title |
---|
이주영, et al., Journal of the Korean Society of Food Science and Nutrition, 2016, vol. 45, no. 4, pp. 492-500 (2016.04.30.) 1부.* |
이주영, 신라대학교 대학원 식품영양학과, 2015, 석사학위 논문. 1부.* |
Also Published As
Publication number | Publication date |
---|---|
KR20220158430A (en) | 2022-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9649351B2 (en) | Anti-angiogenic agents and anti-obesity substances applied with anti-angiogenesis from natural products | |
KR102511361B1 (en) | Composition for Preventing, Improving or Treating Postmenopausal Syndrome Comprising Rosa rugosa Thunberg Extract | |
US20100105766A1 (en) | Composition for inhibition or prevention of bone density reduction | |
JP2022009308A (en) | Composition for ameliorating climacteric symptoms or osteoporosis | |
KR20200136721A (en) | Composition for preventing or treating woman menopause symptoms comprising Cordyceps militaris Concentrate as an active ingredient | |
KR20110131821A (en) | Composition comprising sauchinone as an active ingredient for preventing or treating insulin resistance | |
KR101174701B1 (en) | Food composition, pharmaceutical composition and animal medicine against obesity containing gingerenone a | |
TW201106959A (en) | Composition for preventing or treating irritable bowel syndrome | |
KR20150051845A (en) | Pharmaceutical composition and healthy food for alleviating climacteric syndrome | |
KR102588771B1 (en) | COMPOSITION COMPRISING EXTRACT Colpomenia sinuosa OR Colpomenia peregrina Sauvageau AS AN ACTIVE INGREDIENT FOR ALLEVIATING OR IMPROVING SYMPTOM BY ESTROGEN REDUCING | |
KR102275268B1 (en) | Composition for relieving menopausal symptom or osteoporosis | |
US10806766B2 (en) | Method for treating, preventing, or alleviating osteoporosis | |
KR102068198B1 (en) | Composition containing the extracts or fractions of Circaea mollis Slebold and Zucc for the prevention and treatment of postmenopausal syndrome | |
KR101330687B1 (en) | Composition for treating and preventing obesity containing oriental herbal extracts | |
KR101874460B1 (en) | Composition for preventing, improving or treating depression caused by estrogen secretion decrease comprising Tetragonia tetragonoides extract as effective component | |
KR102347819B1 (en) | Composition for relieving menopausal symptom or osteoporosis | |
KR102427768B1 (en) | Composition containing the extracts or fractions of Agastache rugosa for the prevention and treatment of postmenopausal syndrome | |
KR102541649B1 (en) | A composition for improving, preventing and treating of woman menopause related disease | |
KR20120107025A (en) | Anti-obesity and anti-diabetes composition comprising oriental herbal extracts and fractions | |
KR20170130082A (en) | A Composition Comprising the root extract of Achyranthes japonica NAKAI for preventing or improving the hormonal abnormal syndrome in women | |
EP3449736B1 (en) | Composition for relieving menopausal symptom or osteoporosis | |
KR101330688B1 (en) | Composition for treating and preventing prediabetes and diabetes containing oriental herbal extracts | |
KR101874458B1 (en) | Composition for preventing, improving or treating disorder associated with polycystic ovary syndrome comprising extract of Tetragonia tetragonoides as effective component | |
KR101174702B1 (en) | Food composition, pharmaceutical composition and animal medicine against obesity containing hirsutenone | |
KR20190076437A (en) | Composition for preventing or treating ischemic diseases comprising extract of Tozan as an active ingredient |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |