KR20120101858A

KR20120101858A - The method of mushroom mycelium using of onion and grape

Info

Publication number: KR20120101858A
Application number: KR1020110019933A
Authority: KR
Inventors: 박동국
Original assignee: 박동국
Priority date: 2011-03-07
Filing date: 2011-03-07
Publication date: 2012-09-17

Abstract

PURPOSE: A producing method of mushroom mycelium extract using onion juice and grape juice as a culture medium is provided to replace purified water with the onion juice and grape juice. CONSTITUTION: A producing method of mushroom mycelium extract comprises the following steps: adding a carbon source, a nitrogen source, and mineral salts to onion juice and grape juice to obtain a liquid medium(S1); sterilizing the liquid medium, and injecting Sparassis crispa spawns into the liquid medium(S2, S3); supplying oxygen to the liquid medium by shaking or ventilating, and culturing the spawns(S4); hot-air extracting the obtained mycelium at 105 deg C for 2-12 hours(S5); and packaging the obtained extract(S6). The liquid medium contains 1-30% of onion juice and 1-10% of grape juice. [Reference numerals] (AA) Onion juice, grape juice, a carbon source, a nitrogen source, and minerals; (BB) Sterilizing for 30-120 minutes at 121 deg. C; (CC) Injecting Sparassis crispa spawns in the sterilized condition; (DD) Shake-culturing in 1.5 bar of sterilized air for 5-60 days; (EE) Extracting for 2-12 hours at 105 deg. C; (FF) Obtaining products; (S1) Medium producing step; (S2) Sterilizing step; (S3) Injection step; (S4) Culturing step; (S5) Extraction step; (S6) Packaging step

Description

Method for preparing mushroom mycelium extract using onion juice and grape juice as a medium {The method of mushroom mycelium using of onion and grape}

Mushroom mycelium culture technology

The present invention relates to a manufacturing method designed to increase the taste and consumer preference of the final product in the manufacture of mushroom mycelium beverages generally known as medicinal mushrooms, and more specifically, in cultivating the mushroom mushroom mycelium with onion juice and After culturing the mycelium with grape juice as the main raw material and a method for producing a cultured liquid medium by direct hot water extraction to utilize as a mycelium beverage.

In general, onion has a spicy taste lowers the blood sugar level, has the functionality of the plant (healthy, effective in preventing and improving disease), and sulfur-containing components play a role in lowering blood sugar. Cycloalin facilitates blood circulation such as thrombolysis and coagulation inhibition, and prevents myocardial infarction and cerebral infarction, and thiosirpinet, one of onion's ingredients, causes tears. The treatment is known to be good. In addition, it typically acts as a bactericidal and antibacterial.

In general, the zinnia mushroom is a mushroom of the basidiomycetes, Pleurotus eryngii, and the scientific name is' Sarassis Crispa Wulf. ex Fr.`. It is native to Korea, Japan, Europe, North America, Australia, etc., and grows mainly on stumps and dead trees.

The flower mushroom is 10 30cm in diameter, fruiting body is 10 25cm in height, and the bottom is a common sack with thick stems, and the top is flat with several branches and the edge is wavy. .

The fruit layer of the flower mushroom was developed on the back of the petal, and the bag was short, blunt and hard with a length of 2 5 cm and a butterfly 2 4 cm. The tissue is white, spores are 5 7, butterfly 3 5, oval or oval, flat, and spores white.

The blossom mushroom contains 43.6 out of 100 beta-glugan, so it may be called a beta-glucan lump. The beta-glugan is a key component to increase the body's immunity, and corrects the body's immune system, cancer, hypertension, and sugar. bottle

Turned out to rule. In addition, the blossom mushroom is known to not only lower blood pressure or return blood sugar levels to normal, but also have a hematopoietic effect in addition to excellent anti-cancer effects, but efforts to artificially cultivate it have been carried out in a very small amount that can be captured in a natural state. come.

Other mushrooms belonging to basidiomycetes were mainly used pharmacologically. Ganoderma lucidum belonging to the anger, as well as shiitake mushrooms (Lentinus edodes), cloud fingering (Coriolus versicolor) have been used. In addition, mushrooms are mostly used for food, except for toxic substances, and are expected to replace meat in the future as high protein foods. In particular, according to various recent reports, glucan (glucan), which is a polysaccharide contained in mushrooms, and its derivatives are known to have anti-cancer effects. Korean Journal of Mycology, 8: 107 (1980); Chiraha et al., Fractionation and purification of the polysaccharides with marked antitumer activity from Lentinus edodes (Berk.). Cancer Res. 30: 2776-2781 (1970); Kang, Chang-Jin et al., Studies on the Anticancer Compounds of Korean Basidiomycetes, Mycelial Growth and Anticancer Constituents of Rhizome. Korean Journal of Biochemistry, 14: 101 (1981); Yamamura et al., A selective inhibitor of myxovirus from shiitake (Lentinusedodes). Mushroom Sci. 9 (1): 495-507 (1976)], and mushrooms have attracted much attention as health foods as well as normal food substitutes.

As a method for obtaining the physiological substance of the mushroom, there is also a method of harvesting and taking fruiting bodies, but there is also a method of obtaining the useful components by culturing the mushroom mycelium in large quantities. In general, mycelium has 16 times more nutrients and physiologically active substances than fruiting bodies, and is used in various health foods and medicines. During the physiological activity of mycelium, the immune system is active, and many other activities are presented at various conferences. It is becoming.

On the other hand, Japanese Patent Laid-Open No. Hei 11-56098 discloses a method of cultivating the blossom mushrooms using a medium containing nutrients after removing the water-soluble components by hot water extraction of larch. However, the method of cultivating the mushroom mushroom using the medium by hot water extraction of larch has not only been effective in removing the mycelial growth inhibiting component, but also had the problem of removing the beneficial components when removing the mycelial growth inhibiting component. In addition, the mycelial growth slowed down productivity, and there were many inconveniences in supplying larch.

In addition, the Republic of Korea Patent Publication No. 2002-48337 'Artificial cultivation method of blossom mushroom' adds activated carbon and activated calcium to the medium mixed with larch and broadleaf tree, and the medium containing nitrogen source commonly used at high temperature and high pressure After sterilization, artificial cultivation of the flower mushroom using the temperature change when cultivating the flower mushroom mycelium has been disclosed.

However, the artificial cultivation method of the blossom mushroom as described above had a problem that a large number of mycelia during the incubation period did not develop into the fruiting body primordial or fruiting body, the fruiting body hypertrophy growth. There was a problem that the size of the fruiting body produced due to the lack of timely conditions.

Publication No. 10-2005-0004717 Mycelium beverages and a method for producing the same are provided with a method of cultivating the mycelium mushroom mycelium that can be produced in large quantities while solving the above problems and shortening the cultivation period of the blossom mushroom by using a liquid medium as a raw material. have.

As mentioned above, the most important thing in the culture of the mushroom mushroom mycelium after the mass culture is the most important thing, while cultivating the mushroom mushroom mass safely without contamination. In this way, containing onion juice in the medium due to the natural antibacterial activity of mushroom mycelium cultivation process lowers the rate of culture and improves the taste of the final product. Drinks that customers can easily reach and drink all the time can be preferred to customers because they are safer and feature-rich. Accordingly, an object of the present invention is to provide a medicinal mushroom mycelium culturing method for improving the taste of the final mycelium cultivation solution according to consumer preferences while culturing in large quantities so as not to be contaminated while reducing the cultivation period of the blossom mushroom.

Therefore, in the present invention, in mass culture of the mycelium of medicinal mushrooms, by mixing onion juice and grape juice as the main raw materials of the medium instead of the purified water contained in the medium, the culture period of the same mycelium is more preferred to customers in the texture of the final mycelia culture solution. Taste and further psychologically, it can have a differentiation that can be judged as a safe and functional mycelium extract using natural raw onions and grapes.

In order to achieve the above object, the present invention provides a method for culturing mushroom mycelium, liquid medium production process of adding onion juice and grape juice as a main raw material instead of purified water and lacking carbon source, nitrogen source, inorganic salts; A sterilization process for sterilizing the liquid medium prepared in the liquid medium manufacturing process at 121 for 30 to 120 minutes; Spawn inoculation process of inoculating the flower mushroom spawn in a sterile medium after sterilization; A culture step of culturing the mycelium for 5 days to 60 days while supplying oxygen to the liquid medium in which the seed is inoculated by the seed seed inoculation process by shaking or ventilation; Hot water extracting the mycelium infested in the liquid medium by the culture process for 2 to 12 hours at 105; The purpose of the present invention is to provide a method for producing an extract of the mycelium mushroom mycelium cultured with a medium of onion juice and grape juice, which is characterized by the packaging process of the extract of hot water extraction.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a flow chart showing a method of cultivating the mushroom mycelium with onion juice and grape juice as a main raw material according to a preferred embodiment of the present invention.

(1) liquid medium manufacturing process

The liquid medium manufacturing process (S1) is 20L onion juice and 3L grape juice in a 100L tank, 1.5kg of glucose as a carbon source, 100g of yeast extract as a nitrogen source, 20g KH ² P0 ⁴ 20g and 20g MgSO ⁴ 7H ² O as an inorganic salt It is a process for preparing a liquid medium by adding 10 to 20ml of vegetable defoaming material for food.

(2) Sterilization Process

Sterilization (S2) is a process of sterilizing the mixed medium for 121 to 30 minutes to 120 minutes. At this time, sterilization time should be added or subtracted according to the size of sterilization inside and the capacity of mixed solution.

(3) spawn inoculation process

The spawn seeding step (S3) is a step of inoculating one inoculum of medicinal mushrooms into the sterilized medium through the (S2) process to the liquid medium prepared in the liquid medium manufacturing process (S1). At this time, the inoculum is inoculated using the inoculum liquid cultured in liquid 300ml ~ 500ml in the Erlenmeyer flask by gluing the mushroom mycelium on the first PDA medium. When the outside air is introduced, the medium itself may be contaminated, which may delay or contaminate the mushrooms.

(4) incubation process

The culturing process (S4) is a process of culturing the spawn while supplying oxygen by shaking or ventilating the liquid medium in which the spawn is inoculated by the spawn inoculation process (S3).

The culturing process (S4) proceeds while supplying sterile treated oxygen to convection the liquid medium. A 0.2 micro filter is used for the aseptically treated oxygen supply.

When the air is introduced in the culture process (S4), the air pressure is adjusted to 1.5bar to inject air into the pneumatic regulator. The incubation period is from 5 days to 60 days.

(5) extraction process

The drying process (S5) is a process of extracting hot water for 2 to 12 hours at 105 temperature by applying heat again to the mycelium cultured as an extraction process. The hydrothermal extraction process allows the mushroom mycelium, the main compound of polysaccharide, to be broken down into low molecular form so that it is easily available to human consumption, and by extinguishing all live mushrooms so that the mycelium no longer propagates, the shelf life can be extended. It is a process.

(6) packing process

The sterilization process (S6) is a process in which the liquid mycelium is productized through a pouch packing machine or a bottle feeder machine through the sterilized pipe immediately after the hot water extraction.

As described above, the method of cultivating medicinal mushroom mycelium based on onion juice and grape juice according to an embodiment of the present invention may increase mycelial growth and shorten the period of mycelial culture by increasing the growth rate. Even when water is drinkable, its taste and flavor are excellent and can be favored by consumers.

1 is a flow chart showing a method for culturing mushroom mycelium mainly containing onion juice and grape juice according to a preferred embodiment of the present invention

(1) liquid medium manufacturing process

(2) Sterilization Process

(3) spawn inoculation process

(4) incubation process

(5) extraction process

The drying process (S5) is a process of extracting hot water for 2 to 12 hours at 105 temperature by applying heat again to the mycelium cultured as an extraction process. The hydrothermal extraction process allows the mushroom mycelium, which is the main compound of polysaccharide, to be broken down into low molecular form so that it can be easily used when ingested by the human body. It is a process.

(6) packing process

none

Claims

In the culture method of mushroom mycelium,
Producing a liquid medium in which onion juice and grape juice are used as the main raw materials instead of purified water and insufficient carbon, nitrogen, and inorganic salts are added; A sterilization step of sterilizing the liquid medium prepared in the liquid medium manufacturing process; Spawn inoculation process of inoculating the flower mushroom spawn in a sterile medium after sterilization; A culture step of culturing the mycelium while supplying oxygen to the liquid medium in which the seed is inoculated by the seed seed inoculation process by shaking or ventilation; Hot water extracting again the mycelium in the liquid medium by the culturing process; Onion extracts and pores characterized by the packaging process of the extract after the hot water extraction

The method of claim 1,
Onion juice culture method of the mycelium, characterized in that containing 1 to 30% on the liquid medium of the liquid medium manufacturing process.

The method of claim 1,
Grape juice on the liquid medium of the liquid medium manufacturing process characterized in that it comprises 1 ~ 10%.

The method of claim 1,
Mushroom mycelium extract production method characterized in that the hot water extraction for 2 ~ 12 hours at 105 temperature when the mycelium extract again