CN101058793A - Method of domesticating, cultivating and enriching selenium-rich phellinus igniarius strain - Google Patents

Abstract

The invention relates to a domestication, cultivation, concentration method of selenium morin bacterial. Culture medium is manufactured by 78g mulberry chip, 20g bran, 1g magnesium sulfate, 1g gypsum, 40mg sodiun selenite per Kg material according to water and material with 1:1. 2-1. 3. The method ascertains concentration of selenium according to the enriched ability of the morin bacterial; the content of selenium adds step by step fit for bacterial until the hypha changes more white, strong and the growth isn't limited; improves the selenium content of fruiting body. The invention improves the effect of beauty treatment, health care and medical treatment, and the value in use of selenium morin bacterial is very high.

Description

Selenium-rich phellinus igniarius strain domestication, cultivation, enriching method

Technical field

The present invention relates to a kind of strain domestication, cultivation, enriching method that in the Phellinus bacterium, adds a kind of trace element " selenium ".

Technical background

Selenium is to be found by Swissification scholar Berzalius in 1818 as a kind of chemical element, the ordination number of selenium is 34, in recent years, as can't the synthetic trace element in a kind of human body, the nutrient research of selenium has become countries in the world, one of focus that especially American-European inferior various countries micro-element nutrition research is of greatest concern.Selenium can participate in the catalyzed reaction of enzyme, and enhancing body immunizing power participates in the blocking-up free radical reaction, is the protective material of antagonism toxic substance in the body, has anti-cancer function.

The modern science name of Phellinus is called the Bao Shi shelf fungus, also is called mulberry ear, pin cracked feet, P .linteus and waits until, is a kind of fungi that parasitizes on the mulberry tree of rotting, and belongs to the fungi of Basidiomycotina, Aphyllophorales, polyporaceae, and is very rare at nature.The Compendium of Material Medica record can be controlled the addiction drink and gather, the stomachache metal-inflicted wound.After the mid-90 in 20th century, the researchist of Korea S and Japan finds: the extract of Phellinus has resistancing action to tumour etc.A kind of method of adding trace element " selenium " in the Phellinus bacterium is not arranged at present as yet.

Summary of the invention

The object of the present invention is to provide a kind of selenium-rich phellinus igniarius strain domestication, cultivation, enriching method; this method is the accumulation ability according to the Phellinus bacterium; determine the concentration that applies of selenium; in the domestication process of bacterial classifications at different levels; the applied amount of selenium increases step by step to reach bacterial classification and progressively adapts to; until mycelia dense white, sturdy, the growth do not suppressed, improve the selenium content of sporophore greatly.

For achieving the above object, the present invention is achieved through the following technical solutions: a kind of selenium-rich phellinus igniarius strain domestication, cultivation, enriching method, and this method may further comprise the steps:

1) rich selenium Phellinus one-level kind, promptly female making method of planting: carry out separate tissue with the PDA method by the Phellinus sporophore and cultivate, the production godmother's kind of informing against, plant by first godmother again and be transferred in the level liquid seed bottle, its prescription is potato 200 grams, glucose 20 gram, albumen 3 gram, sal epsom 1 gram, potassium primary phosphate 1 gram, salt 1 gram, VB1,25 milligrams, yeast powder 3 grams, 20 milligrams of Sodium Selenites, mulberry wood chip 100 restrain, 1000 milliliters in water; After earlier wood chip being boiled 15 minutes in 100 ℃ of boiling water, potato is cleaned and goes eye to cut into pieces, put into well-done mulberry wood chip water, potato is boiled to thoroughly well cooked but not mushy elimination wood chip and potato flakes, supply 1000 ml waters, again all the other materials are put into the bottle plastic cloth and sealed, carry out autoclaving; When pot inner pressure reaches 0.05MPa, put clean freezing air, after pointer returns zero, again rising to 121 ℃～126 ℃ kept 30 minutes, pointer returns and insert female the kind when zero back taking-up is reduced to below 30 ℃ in inoculation, cultivated 6～7 days for 25 ℃ at dark, constant temperature, cleaning-less bacteria infection is promptly turned out the female kind of rich selenium Phellinus on inspection;

2) rich selenium Phellinus secondary kind, i.e. original seed making method: mulberry wood chip 78 gram, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 30mg/ kilogram material, material and water are than 1: 1.2～1.3; Earlier wood chip, wheat bran, gypsum are mixed and mix thoroughly, again with sal epsom, Sodium Selenite spice soluble in water, water adds by standard, pH value about 6.5, and secondary kind third rare plastics bag or drip bottle are produced, and seal with cotton; Sterilising method is that heated pressure is when reaching 0.05MPa, discharge freezing air, pointer is warming up to 121 ℃～126 ℃ after returning zero again, keep 2 hours truce self-zeroings to take out, when bacterium bag or bottle were reduced to below 30 ℃, aseptic situation inserted the female kind of level liquid in inoculation tank, and inoculum size is 10 milliliters, normally 25 ℃ of unglazed cultivations in the left and right sides, it is switchable three grades of kinds that 25 days left and right sides mycelia are covered with;

3) three grades of kinds of rich selenium Phellinus, i.e. cultivar making method: mulberry wood chip 78 grams, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 40mg/ kilogram material, all the other are with the secondary kind.

4) selenium-rich phellinus igniarius production process: rich selenium Phellinus cultivating method is packed identical with three grades of kinds, but sterilization high pressure or normal pressure, and autoclaving is with three grades of kind sterilizations, the normal-pressure sterilization kettle temperature reaches 100 ℃, kept 20 hours, inoculation when treating below bag temperature drop to 30 ℃ is normally produced in mid-March;

5) mycelial growth process: selenium-rich phellinus igniarius is placed under 20 ℃～25 ℃ room temperatures and cultivates after producing the bag inoculation, and ventilation condition that relative humidity 65% left and right sides is unglazed is cultivated, and it is 30～40 days that mycelia is covered with the bacterium bag time;

6) sporophore growth management: after mycelia is covered with the bacterium bag, change the physiology growth phase over to, at this moment temperature is controlled at 18～28 ℃, and certain temperature difference be arranged, and humidity is 85～95%, require airiness, stronger scattered light generally forms sporophore through about 15 days, sporophore needs about 20 days from beginning to be formed into maturation, naturally dry or dry after gathering, put into the plastics bag keeping.

The invention has the advantages that: this method is the accumulation ability according to the Phellinus bacterium; determine the concentration that applies of selenium; in the domestication process of bacterial classifications at different levels; the applied amount of selenium increases step by step to reach bacterial classification and progressively adapts to; until mycelia dense white, sturdy, the growth do not suppressed, improve the selenium content of sporophore greatly.Can improve the effect of aspects such as beauty treatment, health care, medical treatment.The Phellinus bacterium also is the best medicinal fungus of present internationally recognized biological anticancer curative effect, and rich selenium Phellinus exceeds several times than Phellinus train chief using value.

Embodiment

A kind of selenium-rich phellinus igniarius strain domestication, cultivation, enriching method, this method may further comprise the steps:

1) rich selenium Phellinus one-level kind, promptly female making method of planting: carry out separate tissue with the PDA method by the Phellinus sporophore and cultivate, the production godmother's kind of informing against, plant by first godmother again and be transferred in the level liquid seed bottle, its prescription is potato 200 grams, glucose 20 gram, albumen 3 gram, sal epsom 1 gram, potassium primary phosphate 1 gram, salt 1 gram, VB1,25 milligrams, yeast powder 3 grams, 20 milligrams of Sodium Selenites, mulberry wood chip 100 restrain, 1000 milliliters in water; After earlier wood chip being boiled 15 minutes in 100 ℃ of boiling water, potato is cleaned and goes eye to cut into pieces, put into well-done mulberry wood chip water, potato is boiled to thoroughly well cooked but not mushy elimination wood chip and potato flakes, supply 1000 ml waters, again all the other materials are put into the bottle plastic cloth and sealed, carry out autoclaving; When pot inner pressure reaches 0.05MPa, put clean freezing air, after pointer returns zero, again rising to 121 ℃～126 ℃ kept 30 minutes, pointer returns and insert female the kind when zero back taking-up is reduced to below 30 ℃ in inoculation, cultivated 6～7 days for 25 ℃ at dark, constant temperature, cleaning-less bacteria infection is promptly turned out the female kind of rich selenium Phellinus on inspection;

2) rich selenium Phellinus secondary kind, i.e. original seed making method: mulberry wood chip 78 gram, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 30mg/ kilogram material, material and water are than 1: 1.2～1.3; Earlier wood chip, wheat bran, gypsum are mixed and mix thoroughly, again with sal epsom, Sodium Selenite spice soluble in water, water adds by standard, pH value about 6.5, and secondary kind third rare plastics bag or drip bottle are produced, and seal with cotton; Sterilising method is that heated pressure is when reaching 0.05MPa, discharge freezing air, pointer is warming up to 121 ℃～126 ℃ after returning zero again, keep 2 hours truce self-zeroings to take out, when bacterium bag or bottle were reduced to below 30 ℃, aseptic situation inserted the female kind of level liquid in inoculation tank, and inoculum size is 10 milliliters, normally 25 ℃ of unglazed cultivations in the left and right sides, it is switchable three grades of kinds that 25 days left and right sides mycelia are covered with;

3) three grades of kinds of rich selenium Phellinus, i.e. cultivar making method: mulberry wood chip 78 grams, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 40mg/ kilogram material, all the other are with the secondary kind.

4) selenium-rich phellinus igniarius production process: rich selenium Phellinus cultivating method is packed identical with three grades of kinds, but sterilization high pressure or normal pressure, and autoclaving is with three grades of kind sterilizations, the normal-pressure sterilization kettle temperature reaches 100 ℃, kept 20 hours, inoculation when treating below bag temperature drop to 30 ℃ is normally produced in mid-March;

5) mycelial growth process: selenium-rich phellinus igniarius is placed under 20 ℃～25 ℃ room temperatures and cultivates after producing the bag inoculation, and ventilation condition that relative humidity 65% left and right sides is unglazed is cultivated, and it is 30～40 days that mycelia is covered with the bacterium bag time;

6) sporophore growth management: after mycelia is covered with the bacterium bag, change the physiology growth phase over to, at this moment temperature is controlled at 18～28 ℃, and certain temperature difference be arranged, and humidity is 85～95%, require airiness, stronger scattered light generally forms sporophore through about 15 days, sporophore needs about 20 days from beginning to be formed into maturation, naturally dry or dry after gathering, put into the plastics bag keeping.

Claims (1)

1, a kind of selenium-rich phellinus igniarius strain domestication, cultivation, enriching method, this method may further comprise the steps:

1) rich selenium Phellinus one-level kind, promptly female making method of planting: carry out separate tissue with the PDA method by the Phellinus sporophore and cultivate, the production godmother's kind of informing against, plant by first godmother again and be transferred in the level liquid seed bottle, its prescription is potato 200 grams, glucose 20 gram, albumen 3 gram, sal epsom 1 gram, potassium primary phosphate 1 gram, salt 1 gram, VB1,25 milligrams, yeast powder 3 grams, 20 milligrams of Sodium Selenites, mulberry wood chip 100 restrain, 1000 milliliters in water; After earlier wood chip being boiled 15 minutes in 100 ℃ of boiling water, potato is cleaned and goes eye to cut into pieces, put into well-done mulberry wood chip water, potato is boiled to thoroughly well cooked but not mushy elimination wood chip and potato flakes, supply 1000 ml waters, again all the other materials are put into the bottle plastic cloth and sealed, carry out autoclaving; When pot inner pressure reaches 0.05MPa, put clean freezing air, after pointer returns zero, again rising to 121 ℃～126 ℃ kept 30 minutes, pointer returns and insert female the kind when zero back taking-up is reduced to below 30 ℃ in inoculation, cultivated 6～7 days for 25 ℃ at dark, constant temperature, cleaning-less bacteria infection is promptly turned out the female kind of rich selenium Phellinus on inspection;

2) rich selenium Phellinus secondary kind, i.e. original seed making method: mulberry wood chip 78 gram, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 30mg/ kilogram material, material and water are than 1: 1.2～1.3; Earlier wood chip, wheat bran, gypsum are mixed and mix thoroughly, again with sal epsom, Sodium Selenite spice soluble in water, water adds by standard, pH value about 6.5, and secondary kind third rare plastics bag or drip bottle are produced, and seal with cotton; Sterilising method is that heated pressure is when reaching 0.05MPa, discharge freezing air, pointer is warming up to 121 ℃～126 ℃ after returning zero again, keep 2 hours truce self-zeroings to take out, when bacterium bag or bottle were reduced to below 30 ℃, aseptic situation inserted the female kind of level liquid in inoculation tank, and inoculum size is 10 milliliters, normally 25 ℃ of unglazed cultivations in the left and right sides, it is switchable three grades of kinds that 25 days left and right sides mycelia are covered with;

3) three grades of kinds of rich selenium Phellinus, i.e. cultivar making method: mulberry wood chip 78 grams, wheat bran 20 grams, sal epsom 1 gram, gypsum 1 gram, Sodium Selenite 40mg/ kilogram material, all the other are with the secondary kind.

4) selenium-rich phellinus igniarius production process: rich selenium Phellinus cultivating method is packed identical with three grades of kinds, but sterilization high pressure or normal pressure, and autoclaving is with three grades of kind sterilizations, the normal-pressure sterilization kettle temperature reaches 100 ℃, kept 20 hours, inoculation when treating below bag temperature drop to 30 ℃ is normally produced in mid-March;

5) mycelial growth process: selenium-rich phellinus igniarius is placed under 20 ℃～25 ℃ room temperatures and cultivates after producing the bag inoculation, and ventilation condition that relative humidity 65% left and right sides is unglazed is cultivated, and it is 30～40 days that mycelia is covered with the bacterium bag time;

6) sporophore growth management: after mycelia is covered with the bacterium bag, change the physiology growth phase over to, at this moment temperature is controlled at 18～28 ℃, and certain temperature difference be arranged, and humidity is 85～95%, require airiness, stronger scattered light generally forms sporophore through about 15 days, sporophore needs about 20 days from beginning to be formed into maturation, naturally dry or dry after gathering, put into the plastics bag keeping.