KR20120094792A - Producing equipment of grafted seedlings and rooted cuttings, and producing method thereof - Google Patents

Abstract

PURPOSE: Producing method and apparatus for rooted cuttings or grafted nursery plants are provided to improve the rooting rate of the cuttings and the nursery plants. CONSTITUTION: A producing apparatus for rooted cuttings or grafted nursery plants comprises the following: a sealed type plant producing chambers(10) enabling users to control the environment including the temperature and the humidity; plural rooting boxes(20) with an opening/closing hole for locating the grafted nursery plants or the rooted cuttings, installed inside the plant producing chambers; and a controller controlling the relative humidity and the lightness inside the rooting boxes.

Description

Producing equipment of grafted seedlings and rooted cuttings, and producing method

The present invention relates to a grafting and cutting seedling production apparatus and a production method, and more particularly to a grafting and cutting seedling production apparatus and production method to increase the sticking rate of the seedlings, shorten the sticking period and improve the quality.

Grafting is a method of producing grafted seedlings by attaching a plant's eye or an attached stem (scion) to a rooted stem or root (rootstock), which is called grafting. Seeds produced through the process are called grafted nursery plants.

The grafted tree and the reception are closely adhered to each other by the oily tissue formed in the formation layer of the connecting part, and the vascular tube is connected to grow into a complete plant.

Cutting is a nutritional breeding method in which a part of the plant nutritional organ is separated from the mother, inserted into the soil or sand, rooted and germinated to become an independent plant. The rooting, shoveling, and leaf shoveling And the like.

Recently, as the number of crops is increased in the same land, serial disturbances such as salt accumulation and soil disease are becoming serious problems. In the growing of vegetables, such as red peppers, tomatoes, and cucumbers, increasing the number of crops and increasing the number of soil diseases such as late blight, green blight, etc., due to long-term cultivation, requires much effort and expense to control the disease. Therefore, in order to control soil diseases, along with soil disinfection and rotation, the production of grafting and cutting seedlings using disease resistant trees is increasing.

However, the production of grafting and cutting seedlings requires skilled skills for grafting work and requires intensive environmental management techniques for the grafting of seedlings after grafting.

If the environmental management is poor in the production of grafting and cutting seedlings, the plants are painted and diseased, and problems such as elevated complex ignition after planting (eg, tomatoes) occur. Painting means that plants grow taller and weaker overall. When plants are painted, normal growth is difficult and it is difficult to get enough harvest.

In particular, the vegetable plug trays grown at high density have a narrow range of regular drop-offs, but when the purchase of seedlings is delayed due to the formal delay of the cultivator, problems such as aging of the seedlings, poor sticking after planting, and delayed growth are caused.

In addition, there is a lack of seedling maintenance technology due to formal delay, damage caused by the use of chemicals such as growth inhibitors, and the problem of developing a storage and distribution environment management technology for maintaining seedlings during distribution of seedlings.

For example, most of the current grafting of grafted seedlings is carried out in tunnels made of PE film and shading film in the greenhouse, and until 2-3 days after grafting, the PE film is sealed to prevent falsification of the grafted seedlings and promote their sticking. After 5 days, the PE film is opened and closed to purify the external environment.

By the way, the sliding environment management of the grafting seedlings such as PE film opening and closing is made by the experience of the manager according to the season or weather, there is a problem that requires skilled skills.

In addition, after the grafting, a closed period of 3-4 days to prevent falsification by transpiration and to promote swelling, the amount of light in the tunnel is saturated at 50 μmol m ^-2 s ^-1 or less and relative humidity of 90% or more. And the airflow is close to 0 ms ⁻¹ .

Photosynthesis rate of nearly ₁ mg CO _{2 m -2} s _-1 has been reported in the management of active environment according to the practice of grafting tomato seedlings. Grafting seedlings under these environmental conditions are often vulnerable to environmental stress and require postpurification purification.

In addition, although most of the grafted seedlings can be adhered to by the sticking environment management method of this practice, problems such as the painting of stems and lobes, the occurrence of irregular roots at the reception, and the occurrence of diseases can be caused.

Accordingly, in recent years, growth inhibitors such as triazole-based pesticides have been used to prevent excessive coating of stems and foliar diseases during grafting.

In addition, during the late acclimation period in which the opening and closing of the greenhouse tunnel is performed, the opening and closing of the tunnel is performed by seasons, weather, or experiences of the manager, and thus it takes 4 to 7 days to complete the acclimatization. Therefore, depending on the season, weather, and management method, the curing and rooting period is 7-10 days, which is wider, so the simplification period is required.

An object of the present invention is to provide a grafting and cutting seedling production apparatus and production method capable of high-density precision environmental control to increase the sticking rate of the seedlings, shorten the sticking period and improve the quality.

According to a feature of the present invention for achieving the object as described above, the present invention is a hermetic plant production chamber capable of environmental control including temperature, humidity; A plurality of sliding boxes positioned in the plant production chamber and provided with openings and openings to be completely sealed or semi-sealed with a transparent material on the front or top surface, and having a grafting seedling or a cutting seedling disposed therein; Control means for controlling the brightness and relative humidity inside the sliding box.

The sliding box is provided with an air inlet on one side, the air inlet is connected to the humidification tube for humidification.

The control means includes a humidifier provided at the air inlet side, a light source provided on the upper part of the sliding box, a detection sensor provided in the sliding box, and a signal selected from the humidifier and the light source by receiving a signal from the detection sensor. It includes a control unit for controlling more than one species.

The sliding box is located in the plant production chamber in the form of a multistage bed.

The grafting seedlings or cutting seedlings are placed in the sliding box during the sliding period, and the sliding box is placed in the plant production chamber set to a set temperature, and then, the light intensity and the relative humidity in the sliding box are adjusted using a light source and a humidifier.

The luminous intensity in the cringing box is maintained at a high luminous intensity (160 to 260 PPF (μmol ^-2 s ^-1 )) during the sliding period.

The grafted seedlings graft the reception to the cotyledons.

The relative humidity in the sliding box is 75-95%.

Opening and closing the opening and closing provided on the front or top surface of the sliding box for dehumidification or carbon dioxide supply in the sliding box.

Grafting and cutting seedling production apparatus of the present invention is possible to implement a variety of sliding environment according to the characteristics of the crop, it is possible to control the high degree of environmental precision has the effect of shortening the sticking period and improve the quality of the seedling.

In particular, the graft and seedling production method of the present invention is a high brightness (160 ~ 260PPF (μmolm ^-2 s ^-1 )), the relative humidity is in the range of 75 ~ 95% to implement the slippery environment is possible to produce an efficient plug tray and sticking This is promoted and the quality of the seedlings without the use of pesticides is improved, there is an effect that can produce high quality seedlings without pesticides.

1 is a block diagram showing a preferred embodiment of the graft grafting seedling production apparatus according to the present invention.
Figure 2 is a schematic perspective view showing a state grafting seedlings arranged in the sliding box of the embodiment of the present invention.
3 is a graph showing the progress of temperature and relative humidity according to the luminous intensity and relative humidity treatment conditions during the fusion and purification of the cucumber grafted seedling of Experimental Example 1.
Figure 4 is a graph showing the carbon dioxide exchange rate (photosynthesis and respiration) according to the light intensity, relative humidity during fusion and purifying.
Fig. 5 is a leaf cross-sectional view of the combined cotyledons and purified cotyledons for 6 days under high light conditions A, medium light conditions B, C dark conditions, D is high light conditions, E is medium light conditions, and F is dark light conditions. Cross section of leaf of accepting cotyledons after seeding for 7 days on glass greenhouse seedling bed after treatment.
FIG. 6 is a graph showing the ratio of dryness, specific leaf area, and ground level to ground level during fusion or purifying grafting or cutting seedlings.
7 is a graph showing the carbon dioxide exchange rate (photosynthesis and respiration) according to the light intensity during tomato graft seedling period.
Figure 8 is a photograph showing the degree of sticking and growth according to the brightness during the sticking period of tomato grafting seedlings.
Figure 9 is a photograph showing the cucumber grafts for each treatment after the end of the graft sliding in Table 10.
10 is a graph showing the change in net photosynthetic rate of cucumber grafted seedlings during treatment during the rooting period of Table 10.

Hereinafter, the present invention will be described in detail.

The grafting seedling production apparatus of the present invention, as shown in Figures 1 and 2, is located in the plant production chamber 10, the plant production chamber 10 and completely sealed or semi-finished with a transparent material on the front or top surface The opening and closing port 23 is provided to enable sealing, and includes a plurality of sliding boxes 20 in which a grafting seedling or cutting seedling m is disposed therein, and control means for controlling the brightness and relative humidity inside the sliding box 20. do.

The plant production chamber 10 is formed in a closed type capable of environmental control such as temperature and humidity control and carbon dioxide supply. The supply of carbon dioxide is carried out through a carbon dioxide supply unit 41 disposed outside the plant production chamber 10 and supplying carbon dioxide into the plant production chamber 10, and the brightness, temperature, and humidity control of the sliding box will be described below. It can be carried out through a light source, a heater, a humidifier.

Sliding box 20 has an air inlet 21 on one side and the air inlet 21 has a structure connected to the humidification pipe 25 for humidification.

The opening and closing port 23 is provided for supplying dehumidification or carbon dioxide in the sliding box 20. For example, the opening and closing port 23 may be provided by configuring the front of the sliding box 20 in a sliding type or a sliding type.

The graft-cutting seedling m is disposed in the sliding box 20 in the form of a plug seedling using a plug tray so as to be seeded at high density.

The control means includes a humidifier 27 provided at the air inlet 21 side of the sliding box 20, a light source 37 provided at the upper part of the sliding box 20, and a sensing sensor provided in the sliding box 20. (33) and a control unit 35 for receiving a signal from the detection sensor 33 to control at least one selected from the humidifier 27 and the light source 37.

Humidifier 27 is for adjusting the relative humidity in the sliding box 20. The air inside the sliding box 20 is increased in humidity by turning on a humidifier switch (not shown).

In detail, when the humidifier 27 provided at the air inlet 21 side is turned on in the process of introducing the air into the sliding box 20, water is contained in the air flowing into the sliding box 20. Humidification is achieved.

Moisture contained in the air can be removed by opening the opening and closing 23 provided in the front or side of the sliding box 20, and the like.

The light source 37 is for luminous intensity control of grafting and cutting seedling m. The light source 37 corresponds to an artificial light source, and for example, a high pressure sodium lamp, a metal halogen lamp, a fluorescent lamp, or a light emitting diode (LED) may be used. Photosynthesis, sticking and growth of grafting and cutting seedling m under artificial light source 37 are promoted with increasing light intensity.

The natural light source (sun) is not preferable because it increases the drying phenomenon when grafting and cutting seedlings.

When the grafting and cutting seedlings in the greenhouse are active, the natural light source increases the temperature inside the greenhouse and decreases the relative humidity to increase the surface growth of the grafting and cutting seedlings. Therefore, in the case of greenhouses, the light shade is always used to maintain low brightness and prevent excessive temperature rise. However, the use of light shade prevents the photosynthesis of the grafting and cutting seedlings, and the drying phenomenon of the grafting and cutting seedlings increases due to the heat, despite the low brightness caused by the use of the light shield. Increased dryness prevents grafts and seedlings from sticking. Therefore, the grafting and cutting seedling production apparatus that can use the artificial light source instead of the natural light source and control the relative humidity is proposed.

A plurality of light sources 37 may be installed on the upper part of the sliding box 20. For example, a plurality of light sources 37 may be installed to be spaced apart from the upper surface of each sliding box 20 by a predetermined interval.

The luminous intensity level by the light source 37 is adjusted as the number of lamps, and the sliding box 20 is further provided with a photometer for measuring the luminous intensity level. For accurate light level measurement, the photometer may use a photometer having a plurality of sense bar photons.

Each sliding box 20 is provided with a heater 39. The heater 39 is for maintaining the temperature inside the sliding box 20 may be a heater.

The detection sensor 33 provided in the sliding box 20 is a thermocouple and a humidity sensor. The thermocouple measures the temperature inside the sliding box 20 and the humidity sensor measures the relative humidity inside the sliding box 20.

All sensors and photometers, including thermocouples and humidity sensors, are connected to the control unit 35 such as a humidifier 27, a heater 39, a light source 37, and the like. In addition, the control unit 35 includes a data recording device therein, and the data recording device collects all data detected by the sensor 33 and the photometer every hour. The temperature, light intensity, and relative humidity in the sliding box 20 are controlled by the control unit 35 that collects the data.

On the other hand, although not shown, the sliding box may be located in the plant production chamber in the form of a multi-stage bed (multi-layer shelf). In this case, an artificial light source is installed at the top of each sliding box so that the brightness in the sliding box can be adjusted.

For reference, reference numeral 31 is a support for fixing the light source 37, 40 is a fan for uniformly mixing the air in the sliding box 20.

Grafting and cutting seedling production method using the above-mentioned grafting and cutting seedling production device is placed in the planting chamber of the grafting seedlings or cutting seedlings (m) in the closed sliding box 20, and the sliding box 20 is set to the set temperature during the sliding period After positioning in (10), using the light source 37, the humidifier 27 to adjust the light intensity and relative humidity in the sliding box 20.

The brightness (brightness of light) in the sliding box 20 is maintained at high brightness (160 to 260 PPF (μmolm ⁻² s ⁻¹ )) during the sliding period. In addition to photosynthetic properties, luminosity also affects plant tissues such as leaf thickness and cell arrangement, resulting in higher photosynthetic capacity at higher luminosity conditions, more chloroplasts in grafted seedlings (m), and thicker leaf thicknesses. Is promoted.

In particular, high luminosity increases the amount of photosynthesis on the leaves of the reception during fusion and purifying after grafting, thus facilitating the grafting and cutting of seedlings (m). Union and purifying are important for slipping because they are related to reinforcement for survival of grafting and cutting seedlings (m), and the intensity, relative humidity, and temperature in the sliding box 20 where grafting and cutting seedlings (m) are located during the sliding period Greatly affects purifying.

Healing means that when a part of a plant is damaged, new cells and tissues are differentiated to cover the upper globules and heal.Acclimatization is the process of adapting to climatic conditions after the plant is transplanted to another land or environment. Means that.

Grafting seedling (m) is preferably grafted to the tree with cotyledons. In the case of cotyledons, the cotyledons increase the CO2 exchange rate, which promotes the vascular bundle linkage between the plants and the plants after grafting.

Relative humidity in the cling box 20 during the cling period is maintained at 75-95% to accelerate photosynthesis.

In general, photosynthesis of plants is promoted at 75-85% relative humidity. High relative humidity above 95% reduces the pore openings in the leaves, reducing photosynthesis. Therefore, the low relative humidity can prevent the pore opening from closing and promote photosynthesis. But too low relative humidity, below 75%, reduces photosynthesis due to moisture stress induced by excessive transpiration of plants.

The above-mentioned grafting and cutting seedling production apparatus and production method can implement a variety of sliding environment to shorten the sticking period of grafting and cutting seedlings and improve the quality of the seedlings to enable the production of high-quality uniform seedlings.

Hereinafter, the present invention will be described in more detail through experimental examples. However, it should be noted that the scope of the present invention is not limited by the following experimental examples.

Experimental Example 1 Cucumber Graft Seedling

a. Reception and preparation of tree tree

Cucumbers are used as a reception and pumpkins are used as a plant to produce grafted seedlings.

Seeds and seedlings are planted in 105-cell and 50-cell plug trays, respectively, and filled with growth medium. Planting density of the receiving passage and are each 694 weeks (plant) m ^-2, 330 ju (plant) m ^-2. This plug tray is placed in a germination chamber maintained at 28 ° C. for 2-3 days to germinate and grow in a plant production chamber with an artificial light source.

The temperature at the time of growth in the plant production chamber is set at 25 ° C./18° C. (light irradiation period (day) / dark period (night)). The light irradiation period provides approximately 200 lmol ⁻² s ⁻² luminous intensity with fluorescent tubes. Provide water or culture at the bottom of the plug tray.

Twice before grafting, measure stem diameter, dry weight of leaf, and leaf area. The results are shown in Table 1 below.

division Stem diameter
(mm) Dry weight of leaves
(mg) Leaf area
(㎠) Before grafting 1.36 ± 0.04 39.35 ± 1.09 11.67 ± 0.23 Before grafting 2.51 ± 0.06 170.05 ± 3.23 27.05 ± 0.76

b. grafting

Grafting is carried out by splice grafting method once a week after sowing and when cotyledons of large tree are fully unfolded. For grafting, the root and one cotyledon and growth point of the tree are removed, and the receptionist cuts 5 mm below the cotyledon. Next, place the reception on the upper part of the tree and fix the grafted position firmly with the commonly used grafting forceps. The grafted seedlings are then inserted into a plug tray containing new topsoil.

c. survival

Place the grafted seedlings in the grafted seedling production equipment.

When the grafting seedling is disposed in the sliding box, the air is contained by turning on the humidifier provided at the air inlet side to humidify the inside of the sliding box, and open and close the opening and closing opening of the sliding box for removing moisture contained in the air and exchanging carbon dioxide.

The intensity, temperature and relative humidity measured by the sensor in each slide box are collected every hour, and the air temperature in the slide box is maintained at 27 ° C using a heater.

11 fluorescent lamps are installed in parallel on the upper part of the sliding box, 20cm away from the top surface, and the distance between each lamp is 1cm. The brightness level is adjusted as the number of fluorescent lamps turned on, and the light irradiation period and the dark period are set to 12 hours, respectively.

d. Processing during control period (control)

The fusion and purification of the grafted seedlings (grafted cucumbers) is carried out in the crates for 6 days. No watering (irrigation) was done during the fusion and purifying periods. For 6 days of fusion and purification, a combination of two levels of relative humidity and three levels of luminosity is designed.

Each level is shown in Table 2. For reference, gourds and plants such as cucumbers require high humidity, so experiment with a relative humidity of 90% or higher. If the relative humidity is less than 90%, it does not stick properly due to the characteristics of the crop.

division H M L Relative Humidity (%) 95 - 90 Luminance (PPF (μmolm ^-2 s ^-1 )) 160 or more and less than 260 100 or more but less than 160 100 or less

For example, high relative humidity and low brightness are denoted HL, and low relative humidity and high intensity are denoted LH.

Table 3 shows an example of relative humidity and light intensity (PPF) treatment during fusion and purification.

Treatment code Relative Humidity (%) Luminance (PPF) (чmolm ^-2 s ^-2 ) HH 95 237 ± 8 HM 95 142 ± 8 HL 95 0 LH 90 237 ± 8 LM 90 142 ± 8 LL 90 0

3 graphically illustrates the temperature and relative humidity treatments of air during fusion and purification.

e. CO2 exchange rate measurement

During the treatment period, the carbon dioxide concentration is measured by sampling a certain amount of air flowing into the air inlet of the sliding box and the air discharged through the opening and closing port, and the carbon dioxide exchange rate of the grafted seedlings is measured.

As a result, the increase in the light intensity (PPF) during the coalescence and purification resulted in the increase of the carbon dioxide exchange rate (the increase in photosynthesis).

As shown in FIG. 4, the carbon dioxide exchange rate was 1.5 and 1.8 times higher in HH and LH treatments than in HM and LM treatments, respectively. On day 6 of HH and LH treatment and HM and LM treatment, the exchange rate of carbon dioxide increased by 2 times compared to the first day.

During dark periods, the CO2 exchange rate was negative by respiration and positively correlated with luminosity. However, at 90-95% relative humidity, relative humidity did not have a significant effect on the CO2 exchange rate during fusion and purification of the grafted seedlings.

The rate of CO2 exchange of cucumber grafted seedlings grafted during grafting or cutting of seedlings is influenced by the luminosity and relative humidity.

f. Microscopic observation

Samples and cross sections are prepared for microscopic observation. Leaf slices are harvested from the cotyledons of the cotyledons received 6 days (6 days sticking period) and 13 days (6 days seedlings in a regular glass greenhouse for comparison between treatments after 6 days gliding) for tissue measurement.

The collected leaf fragments are stained with periodic acid (PAS) after pretreatment such as fixation and drying, and the tissue is observed under an optical microscope.

After pretreatment, put 2.5% glutaraldehyde in a phosphate buffer (pH 7.2) cooled to 4 ° C, load the collected leaf pieces for 2 hours to fix and wash the tissues, and then 1% tetracyclization at 4 ° C. The tissue is fixed by charging it with osmium for 2 hours and keeping it in the phosphate buffer for 1 day. After fixation, drying is carried out in ethyl alcohol stepwise for complete drying.

Microscopic observations show the difference in chloroplast distribution of leaf tissues. Experimental results show that fusion in medium (HM) or dark conditions (HL) and in high light (HH) conditions compared to purified reception. More chloroplasts were observed in the cotyledons of overlying reception. At 13 days after inoculation, the chloroplasts of tissues were increased in both high and low light conditions (HH) rather than medium and dark conditions (HM).

g. Growth

Table 4 shows the growth of fused and purified cucumber grafted seedlings for 6 days (sliding period 6 days) in grafting and cutting seedling production equipment.

Processing code
(Treatment code) Plant ground length (extended)
(cm) Number of leaves Stem diameter
(mm) Leaf area
(㎠) Underground
Dry weight
(mg) Ground
Dry weight
(mg) HH 8.09 0.95 4.08 50.31 39.9 228.9 HM 8.71 1.00 3.85 47.97 30.3 188.0 HL 9.59 - 3.51 36.28 20.0 128.5 LH 7.45 1.47 4.25 48.10 46.2 271.9 LM 7.46 1.57 3.92 45.47 36.8 240.7 LL 9.65 0.55 3.79 38.50 24.8 198.4

Table 5 shows the growth of cucumber grafted seedlings grown in a greenhouse (13 days after grafting) for 6 days after fusion and purification in the grafting and cutting seedling production apparatus.

Processing code
(Treatment code) Plant ground length (extended)
(cm) Number of leaves Stem diameter
(mm) Leaf area
(㎠) Underground
Dry weight
(mg) Ground
Dry weight
(mg) HH 8.40 2.35 5.15 87.57 49.8 434.0 HM 8.66 2.35 4.85 75.45 42.3 372.7 HL 10.00 0.95 4.20 51.37 27.8 290.9 LH 7.08 1.95 4.79 69.16 51.5 356.0 LM 7.85 2.20 4.76 71.13 40.8 304.6 LL 9.36 1.15 4.14 46.72 27.6 249.3

FIG. 6 is a graph showing the building ratio, specific leaf area, and the ratio of the basement to the ground part according to the luminosity during the fusion and purification of the grafting or cutting plants.

The building rate is the ratio of the original organism to the building without water in the original state, and the specific leaf area is the leaf area divided by the weight of the building. The leaf thickness is indirectly expressed. It is ratio in building of basement (root) to building of the ground part.

According to Table 4, Table 5 and Figure 6, as the result of the increase in brightness during the sticking period, the reception length of the grafted seedlings decreased 6 days and 13 days after grafting, but the stem diameter, number of leaves, leaf area, and dry weight of the ground increased. . The relative humidity range of 90-95% did not have a statistically significant effect on the growth of grafted seedlings.

Experimental Example 2 Tomato Grafting Seedling

Reception desk: Super dotaelang, big tree: B blocking

The seedling and seedling conditions for seedlings were set at light irradiation period (daytime): 14 hours, 25 ° C, dark period (night): 10 hours, 20 ° C, and grown on growth.

Two days after the sowing, the seedlings were sown and grafted after 4 weeks.

The treatment was performed to the light intensity and relative humidity during the sticking period (6 days) as shown in Table 6 below.

Treatment Low Medium High Luminance (PPF) (μmolm ^-2 s ^-1 ) 79.8 ± 7.2 123.6 ± 14.3 217.4 ± 26.8 Relative Humidity (%) 75,85,90,95

Looking at Table 6 and Figure 7, Figure 8, the carbon dioxide exchange rate was high at high brightness and the sliding of tomato grafted seedlings was promoted.

Table 7 shows the growth of tomato grafted seedlings according to the luminosity conditions of Table 6 (after 6 days of grafting).

Brightness treatment
(Treatment) chlorophyll
(SPAD) Leaf area
(㎠) Underground
Dry weight (g) Ground
Dry weight (g) Low 41.93a 16b 0.014b 0.051b Medium 40.75b 18ab 0.015ab 0.060b High 39.55c 19a 0.017a 0.079a

DMRT (P = 0.05)

As a result of the experiment of Table 7, the photosynthesis amount increased during the sorption period as the brightness increased. At the end of the solubility, photosynthesis increased by about 3 times in high light condition compared to low light condition. .

Table 8 shows the growth according to the influence of relative humidity during the sticking period after grafting tomatoes. Luminance was maintained at high luminance during the rooting period.

Life span Relative humidity
process
(Treatment) Plant ground length
(Extension)
(cm) Number of leaves chlorophyll
(SPAD) Leaf area
(㎠) Underground
Dry weight
(g) Ground
Dry weight
(g) 6 days 75% → 80% 9.14a 4.0a 40.54a 18.74b 0.019b 0.096a 6 days 80% → 90% 9.04a 3.9a 36.44a 20.17b 0.025a 0.089a 6 days 85% → 95% 9.41a 4.1a 37.96a 26.13a 0.026a 0.113a 13th 75% → 80% 12.4a 5.2a 34.45a 36.69a 0.042a 0.187a 13th 80% → 90% 12.4a 5.2a 34.76a 39.03a 0.044a 0.187a 13th 75% → 80% 11.8b 5.2a 33.95a 36.16a 0.042a 0.182a

DMRT (P = 0.05)

As a result of the experiment of Table 8, the relative humidity ranged from 75% to 95%, but all were promoted.

Table 9 shows the growth during the gliding period when grafts were applied to trees with cotyledons and when grafts were applied to trees without cotyledons.

Experimental conditions: High intensity (217.4 PPF (μmolm ^-2 s ^-1 )), relative humidity (85%), light irradiation period (day): 12 hours, 25 ℃, dark period (night): 12 hours, 20 ℃

division CO2 Exchange Rate / Net Photosynthesis After grafting
1 day After grafting
2 days After grafting
3 days After grafting
4 days After grafting
5 days After grafting
6 days Large tree (Cotyledon x) + Reception -/0 -/0
-/0 + / + + / + + / + Large tree (Cotyledon O) + Reception + / + + / + + / + + / + + / + + / +

As a result of the experiment of Table 9, cotyledons without cotyledons withered with cotyledons until 3 days after grafting, and the CO2 exchange rate was (-) until 3 days after grafting, but increased to (+) after 4 days. From this point, it was found that photosynthesis increased due to the connection of the coronary cortex of the cortex.

In the case of cotyledons, carbon dioxide exchange rate increased from day 1 and net photosynthesis also increased.

Table 10 shows photosynthesis of cucumber grafted seedlings without cotyledons.

process
Before the main leaves of 50-60% of grafted seedlings start to develop After 50-60% of main leaves of the grafting plant begin to develop PPF (μmolm ^-2 s ^-1 ) Relative Humidity (%) PPF (μmolm ^-2 s ^-1 ) Relative Humidity (%) I 176 89 176 79 Ⅱ 176 93 176 84 Ⅲ 176 93 260 84 Ⅳ 260 93 260 84

The results in Table 10 are shown in FIG.

According to Table 10 and FIG. 9, it is confirmed that the sliding is promoted in the cucumber grafting seedlings for each treatment after the grafting sliding of the treatment IV is completed.

Figure 10 shows the change in the net photosynthetic rate of the cucumber grafted seedlings for each treatment during the rooting period of Table 10.

10, the net photosynthesis of the grafted seedlings of Treatment IV increased from day 1, and the net photosynthesis rate was also the highest.

Through this, the cotyledons with cotyledons are used for grafting, and high brightness (160 ~ 260 PPF (μmolm ^-2 s ^-1 )) and relative humidity in the range of 75 ~ 95% during the grafting / cutting of seedlings It can be seen that by realizing the sticking environment, the sticking period of grafting and cutting seedlings can be shortened and the seedling quality can be improved.

Within the scope of the basic technical idea of the present invention, many other modifications are possible to those skilled in the art, and the scope of the present invention should be interpreted based on the appended claims. will be.

10: Plant production chamber 20: Sliding box
21: air inlet 23: opening and closing
25: humidifier 27: humidifier
31: support 33: detection sensor
35: control unit 37: light source
39: heater 40: fan
41: Carbon dioxide supply part m: Seedling (grafting seedling or cutting seedling)

Claims (9)

An airtight plant production chamber capable of environmental control including temperature and humidity;
A plurality of sliding boxes positioned in the plant production chamber and provided with openings and openings to be completely sealed or semi-sealed with a transparent material on the front or top surface, and having a grafting seedling or a cutting seedling disposed therein;
Grafting and cutting seedling production apparatus comprising a control means for controlling the brightness and relative humidity inside the sliding box. The method according to claim 1,
The live box is
It has an air inlet on one side,
Grafting and cutting seedling production apparatus, characterized in that the air inlet is connected to the humidification pipe for humidification. The method according to claim 1,
The control means
A humidifier provided at the air inlet side;
A light source provided at an upper portion of the sliding box,
A detection sensor provided in the sliding box,
Grafting-cutting seedling production apparatus characterized in that it comprises a control unit for controlling the at least one selected from the humidifier, light source by receiving the signal of the sensor. The method according to claim 1,
The graft box is a graft? Seedling production apparatus, characterized in that located in the plant production chamber in the form of a multi-stage bed. During the rooting period, the grafting seedlings or cutting seedlings are placed in the sliding box,
Grafting and cutting seedling production method characterized in that for positioning the sliding box in the plant production chamber set to a set temperature and then controlling the light intensity and relative humidity in the sliding box using a light source and a humidifier. The method according to claim 5,
During the active period
Grafting and cutting seedling production method characterized in that the brightness in the sliding box is maintained at high brightness (160 ~ 260PPF (μmolm ^-2 s ^-1 )). The method according to claim 5,
The grafting seedling is grafting seedlings production method, characterized in that the grafts received in the cotyledons. The method according to claim 5,
Grafting and cutting seedling production method, characterized in that the relative humidity in the sliding box is 75 ~ 95%. The method according to claim 5,
For dehumidification or carbon dioxide supply
Grafting and cutting seedling production method, characterized in that for opening and closing the opening and closing provided on the front or top surface of the sliding box.

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