KR20120084086A - Vegetative propagation method of salix caprea l. using cutting - Google Patents

Abstract

PURPOSE: An asexual propagation method of salix caprea using cuttings is provided to process the cutting with a rooting promotion agent for promoting the rooting of the cuttings. CONSTITUTION: An asexual propagation method of salix caprea comprises the following steps: collecting cuttings from the salix caprea from March 28th to April 14th; processing the cuttings with 90~110mgL-1 of rooting promotion agent for 50-70 minutes; and planting the cuttings on culture soil. The rooting promotion agent is IBA(indole-3-butyric acid).

Description

Vegetative propagation method of Salix caprea L. using cutting}

The present invention belongs to a technique relating to a method of growing a hover worm using cutting. More specifically, in the present invention, the horns ( Salix) extracting the insert from the caprea L. ); Treating a rooting promoter with the collected insert; Inserting the rooting agent-treated insert into cultured soil; It is a description of a method for asexual growth of the horns using a cutting containing a.

They are a deciduous broad-leaved arborescent belonging to the willow tree Willow and Willow, native to Korea, and are distributed in Japan and Siberia. In Korea, it is a wide-area species that grows between 100-2,300m above sea level, and grows up to 6m in height and 15cm in diameter. Leaves are long oval or broad oval, 3-14 cm long, 2-7 cm wide, apex, original or basal, with white villi on the back of leaf. The petiole is 5-30mm long, hairy, and the blade is semicircular and serrated. Fruits are capsules, long egg-shaped, mature in May, flowers are euphoric flowers, apricots bloomed in the previous year before leaves in April, and females are female. The young branch has the hairs and the ovate is egg-shaped, and it is red and has a distinct radiance. As the stem grows, the branches grow thick and the bark becomes gray black.

Most of the willows are characterized by a very wide ecological range, easy nutrition, and rapid growth of branches and roots even in poor soils as precursors. Because of these characteristics, willows have long been used to prevent erosion of river banks, smooth drainage of slopes, slope support, or disaster recovery. Recently, tigers have been used to develop landscapes, stabilize damaged ecosystems, and produce bioenergy. It is emerging as a planting material for harvesting forests or a medical use material for wood components.

Although most willows are naturally fertile, tigers and their hybrids are very difficult to root by traditional cutting techniques.

Studies on the proliferation of tigers include the study of the effect of growth regulator BA on the proliferation of five willow clones, including tigers 1 clone (Bergman et al. 1985) and analysis of tigers 9 clones on tissue culture capacity. There is a result (Neuner and Beiderbeck, 1993). The results of these two studies concluded that asexual growth is very difficult for tigers, and the development of technology for cutting of tigers with high availability is required. Therefore, the inventor of the present invention is to provide a method of asexual growth of the tiger hover using the cutting through the identification of the timing of harvesting, the timing of the insertion, the concentration of the rooting agent to be treated to the cutting and the time of the insertion of the hovering.

An object of the present invention is first to provide a method for asexual growth of the hover using the cutting, and second, to provide a hobber obtained by the asexual growth method.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical subjects which are not mentioned can be clearly understood by those skilled in the art from the description of the present invention .

In the present invention to solve the problems of the prior art horns ( Salix) extracting the insert from the caprea L. ); Treating a rooting promoter with the collected insert; Inserting the rooting agent-treated insert into cultured soil; It provides a hobbling asexual growth method using a cutting comprising a.

Preferably, the harvesting time of the cuttings may be characterized in that March 28 to April 14.

Preferably, the rooting promoter may be characterized in that IBA (indole-3-butyric acid).

More preferably, the treatment concentration of the IBA may be characterized in that 90 ~ 110mgL ^{- 1} .

More preferably, the treatment method of the IBA may be characterized in that it is immersed in the rooting promoter 0.5 ~ 1.5cm of the cutting base taken from the hover.

More preferably, the treatment time of the IBA may be characterized in that 50 to 70 minutes.

Preferably, the insert is 9.8 ± 2.1cm in length, 2 to 4 leaf eyes, it can be characterized in that the shoot of a year-old tiger willow (shoot).

Preferably, the cultured soil may be characterized in that the mixed soil sand (pit moss): vermiculite (vermiculite): pearlite (pearlite) is mixed in a volume ratio of 1: 1: 1: 1.

In another aspect, the present invention provides a horber proliferated by any one of the above methods.

The present invention is to provide a method of propagation of the tigers using the cutting of the asexual breeding method in order to provide a method of propagation of the tigers widely used as a purification and restoration material of the damaged ecosystem around the world.

On the other hand, in the asexual growth method of the hovers using the cutting of the present invention, callus formation rate is higher and the rooting rate is promoted compared to the untreated (control) without the rooting agent treated at the cutting time, the cutting time and the cutting of the hovers. By identifying the concentration and treatment time of the rooting promoter, it is possible to provide an augmentation method for hobbling using optimal cutting.

In particular, the method of growing the horns using the cutting of the present invention uses the cutting, which is an asexual breeding method, so that the traits of the same parameters can be maintained in the tigers as they are, and stable seed production through the production of useful materials and bioenergy production forest It can lead to solving the problem of sexual growth. In addition, the seedling production time can be shortened and mass production at a low cost can greatly contribute to the hordes-related industry.

1 is a photograph showing the horned mature trees, leaves, winter snow, male flowers and female flowers from which the cuttings were taken.
Figure 2 shows the side, rooting and seedlings on the day 60 days after the insertion of the tiger willow using the cuttings taken March 28, 2009.
Figure 3 is a photograph showing the development of the tiger willow seedlings treated with IBA 100mg L ^{-1 for} 1 hour when the tigers were inserted on March 30, 2010.
Figure 4 is a photograph showing the rooting of the horn willow cutting 60 days after IBA 100mgL ^-1 1 minute, 1 hour, 6 hours in the horn willow insertion on March 30, 2010.

The present invention relates to a method for asexual growth of hovers using cuttings, and more particularly, extracting the cuttings from the hoaxes ( Salix caprea L. ); Treating a rooting promoter with the collected insert; Inserting the rooting agent-treated insert into cultured soil; It relates to a hobbling asexual growth method using a cutting comprising a.

Preferably, the harvesting time of the cuttings may be characterized in that March 28 to April 14.

Preferably, the rooting promoter may be characterized in that IBA (indole-3-butyric acid). More preferably, the treatment concentration of the IBA may be characterized in that 90 ~ 110mgL ^-1 . More preferably, the treatment method of the IBA may be characterized in that it is immersed in the rooting promoter 0.5 ~ 1.5cm of the cutting base taken from the hover. More preferably, the treatment time of the IBA may be characterized in that 50 to 70 minutes.

Preferably, the insert is 9.8 ± 2.1cm in length, 2 to 4 leaf eyes, it can be characterized in that the shoot of a year-old tiger willow (shoot).

Preferably, the cultured soil may be characterized in that the mixed soil sand (pit moss): vermiculite (vermiculite): pearlite (pearlite) is mixed in a volume ratio of 1: 1: 1: 1.

In addition, the present invention relates to the hoggers grown by any one of the above methods.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

<Experimental Example>

1. Materials and Methods

Holly willows were harvested on March 6, March 28, April 14, and May 8, 2009, respectively (see Figure 1). The cuttings taken on March 6, March 28, April 14, and May 8, 2009 were cut in the glass greenhouse on that day.

In the above, the cuttings were placed in three repeats of 15 pieces for each period to have a length of 10 ± 3.9 cm to include 1-4 year olds with 2 to 4 leaf eyes.

After cutting, a shading net was installed to prevent the temperature rise in the glass greenhouse, and fine spraying was conducted every hour by using an automatic spraying device during the cutting period (60 days). The average was maintained at 24.4 ± 5.3 ° C. and 72.3 ± 11.2%.

The tiger willows were harvested on March 30, 2010. Cuttings taken on March 30, 2010 On the same day, cuttings were carried out in glass greenhouses.

The insertion was 6.3 ± 0.9 cm long to include 1 year of dough with 2-4 leaf buds.

3, 2010, respectively before 30th cuttings in the cuttings are rooted promoter (IBA) is 0㎎L ^-1 [untreated ^{(control)], 100㎎L -1, 250㎎L -1} , 500㎎L -1, 1000㎎L ^-1, 2000㎎L ^-1, were each treated by 3000㎎L ^-1 concentration, treatment time was the cuttings bed soil to 1 minute, 1 hour, and then for 6 hours and 15 3 each repeated for each concentration.

After cutting, a shading net was installed to prevent the temperature rise in the glass greenhouse, and fine spraying was conducted every hour by using an automatic spraying device during the cutting period (60 days). The average was maintained at 24.2 ± 6.6 ° C and 71.5 ± 12.1%.

The soils used for cutting on March 6, March 28, April 14, May 8, and March 30, 2010, respectively, were sand: peat moss: vermiculite: pearlite, and 1: 1: 1, respectively. A mixed soil mixed in a volume ratio of 1 was used.

The results of the study showed that the survival rate and root length were more than 5 mm after 60 days of cutting, and the rooting rate, rooting number, root length, and biomass characteristics were examined for each treatment.

For data analysis, variance analysis of rooting rate and growth characteristics was conducted for strict analysis of experimental data (SAS, 1996).

2. Cutting result

The average rooting rate for hovers was 3.4%, the lowest on May 8, and 59.5%, the highest on March 28. In other words, it was concluded that hovering was best at the end of March to early April (Table 1). The overall survival rate was 47.7%, the lowest on April 14 with 26.4% and the highest on March 6 with 71.1% (see Table 1). However, after more than 60 days after cutting, only the leaves alive and not rooted were almost killed. In other words, the rooting rate of the hoverers showed a large difference according to the timing of the cutting, and the statistical significance was also high, indicating the importance of the timing of the cutting of the hovering.

Similar to rooting rate and survival rate, root rooting number and length were the highest in March 28 from 8.07 (8.7cm) (see Fig. 2). Was the right time for harvesting tiger willows.

In late March, early April, the time when the tiger willows were harvested, just after the leaves and female flowers of the tigers fell. Particular attention should be paid to the cuttings taken early before the flowering of the tigers, and the flower and leaf eyes are not distinguished. Therefore, after the flowers have completely fallen out, cuttings should only be carried out using leaf buds.

The IBA used to promote rooting of hoists was most suitable for 100mg L ^-1 , 1 hour treatment. IBA showed a rooting rate of 70.8% for 100 mg L ^-1 hour treated hogs, a 25.8% increase from 56.3% for no treatment (see Table 3). In the case of IBA treatment, the rooting rate and survival rate decreased with increasing concentration, and at 250mg L ^-1 or higher, the rooting rate and survival rate decreased with increasing treatment time. In other words, IBA treatment to promote the rooting of the worms is preferred to increase the treatment time at low concentrations of less than 100mg L ^-1 and to shorten the treatment time at high concentrations of 100mg L ^-1 or higher, but IBA is best suited to 100mg L ^-1 1 hour treatment. Judging by the treatment conditions. Root number and length of roots were not different between IBA treatments, but the biomass weight was 1.04g above ground level and 0.31g below ground level in IBA 100mg L- ¹ treatment, which was lower than untreated average (Fig. 3). See, FIG. 4).

As a result of analyzing the effect of IBA rooting promoter concentration and treatment time on rooting, statistical significance was found in IBA concentration, treatment time and interaction (see Table 4). However, statistical significance between repetitions was not recognized, so the difference in cutting rooting rate between each cutting was small.

In conclusion, the asexual growth of hedgehogs using cuttings was determined by the most important factors such as the timing of cutting, the timing of cutting, the concentration of rooting promoter, and the treatment period. In particular, the timing of cutting was most important. It is judged to be. Hardwood is the most suitable for cutting, and it is the most suitable material for the leaves with the leaf buds, not the flower buds.

In addition, it was found that the concentration and treatment time of the rooting accelerator treatment to increase callus formation rate and promote rooting were very important. For IBA, one hour treatment was most suitable at a relatively low concentration of 100 mg L ⁻¹ . In addition, when cutting was performed, better results can be obtained in the irrigation treatment using a light shielding and spraying device, and when asexual growth is performed in cultured soil considering breathability and drainage, it is determined that mass growth of the hoverers is possible.

Table 1 below relates to the rooting characteristics and growth rate for each time the horn willow insertion.

Cutting day Rooting rate
(%) Survival rate
(%) Root Live weight Count Length (cm) Ground part (g) Underground part (g) March 6 37.8 ± 6.7 ^* 71.1 ± 7.7 5.2 ± 1.6 7.0 ± 2.2 1.53 ± 0.80 0.09 ± 0.06 March 28 59.5 ± 9.7 66.4 ± 9.0 8.0 ± 1.6 8.7 ± 2.2 2.76 ± 0.80 0.39 ± 0.06 April 14 21.8 ± 2.6 26.4 ± 3.8 2.8 ± 2.2 8.4 ± 0.9 2.67 ± 2.38 0.18 ± 0.13 May 8 3.4 ± 3.0 27.0 ± 12.6 2.7 ± 2.3 7.1 ± 7.2 0.92 ± 0.86 0.32 ± 0.31

Table 2 below relates to the results of analysis of variance of the rooting rate of cutting according to the timing of extracting the horn willow.

Variable df Mean square F-value Pr> F Collecting time 3 1403.2 77.63 0.0001 Replication 2 13.7 0.71 0.5342

Table 2 below relates to the rooting hormone treatment concentration and rooting characteristics and growth with time.

Hormone treatment Rooting rate
(%) Survival rate
(%) Root Live weight (g) IBA concentration
(mg L ^-1 ) Processing time Count Length (cm) Ground Underground No treatment 0 56.3 ± 6.3 ^* 85.4 ± 7.2 19.7 ± 3.8 10.5 ± 0.9 1.47 ± 0.32 0.56 ± 0.25 100 1 minute 42.0 ± 7.0 71.5 ± 7.8 13.0 ± 5.2 10.8 ± 0.6 1.11 ± 0.21 0.41 ± 0.07 1 hours 70.8 ± 7.2 91.7 ± 3.6 9.3 ± 0.6 9.8 ± 2.1 1.07 ± 0.26 0.27 ± 0.15 6 hours 14.6 ± 7.2 27.1 ± 13.0 17.3 ± 7.8 9.8 ± 0.4 0.94 ± 0.52 0.26 ± 0.18 250 1 minute 60.4 ± 13. 88.9 ± 2.4 20.0 ± 9.2 9.6 ± 1.6 0.56 ± 0.16 0.36 ± 0.11 1 hours 52.1 ± 9.5 83.3 ± 7.2 19.0 ± 2.6 9.3 ± 1.1 0.82 ± 0.22 0.31 ± 0.11 6 hours 17.8 ± 1.7 27.2 ± 7.3 20.7 ± 4.7 9.3 ± 1.1 0.97 ± 0.25 0.29 ± 0.27 500 1 minute 44.6 ± 1.4 59.0 ± 16.8 16.0 ± 3.0 10.5 ± 1.3 0.98 ± 0.26 0.37 ± 0.12 1 hours 25.0 ± 12.5 70.8 ± 13.0 9.7 ± 1.2 8.8 ± 0.7 0.92 ± 0.19 0.33 ± 0.13 6 hours 6.3 ± 0.0 14.6 ± 7.2 18.3 ± 16.3 9.4 ± 2.0 0.85 ± 0.72 0.24 ± 0.17 1000 1 minute 44.1 ± 22.4 63.8 ± 15.2 14.3 ± 7.8 10.9 ± 1.1 1.10 ± 0.55 0.32 ± 0.18 1 hours 11.1 ± 6.7 25.7 ± 26.5 5.3 ± 2.1 5.4 ± 2.1 0.47 ± 0.40 0.06 ± 0.07 6 hours 7.6 ± 4.3 15.3 ± 6.0 16.0 ± 5.6 5.5 ± 3.9 0.44 ± 0.13 0.08 ± 0.08 2000 1 minute 22.9 ± 13.0 45.8 ± 19.1 23.7 ± 3.8 9.9 ± 1.4 0.50 ± 0.07 0.26 ± 0.09 1 hours 10.4 ± 3.6 29.2 ± 7.2 9.0 ± 4.6 11.4 ± 4.5 0.43 ± 0.22 0.16 ± 0.10 6 hours 1.4 ± 2.5 8.5 ± 3.9 10.3 ± 4.0 2.8 ± 1.0 0.23 ± 0.05 0.02 ± 0.01 3000 1 minute 18.8 ± 0.0 43.8 ± 12.5 14.0 ± 5.6 7.8 ± 3.3 0.65 ± 0.04 0.16 ± 0.17 1 hours 14.6 ± 15.7 58.3 ± 20.1 8.7 ± 1.2 8.3 ± 0.0 0.42 ± 0.16 0.10 ± 0.05 6 hours 6.3 ± 10.8 14.6 ± 7.2 11.7 ± 8.5 6.0 ± 2.7 0.28 ± 0.21 0.10 ± 0.10

Mean ± Standard Deviation

 Table 4 below relates to variance analysis results of rooting hormone treatment concentration and rooting rate over time.

Variable df Mean square F-value Pr> F Concentration (A) 6 1887.8 21.40 0.0001 Time (B) 2 4269.6 48.40 0.0001 Replication 2 145.5 1.65 0.2064 A × B 10 483.4 5.48 0.0001

&Lt; Example 1 >

The hoggers used to collect, transplant, and manage the indigenous individuals from the waste-rock pile of Yeonhwa Mine in Bonghwa-gun, Gyeongbuk.

On March 6, March 28, April 14, and May 8, 2009, cuttings were taken from the tigers managed by the Forest Resources Development Department of the Korea Forest Research Institute.

The tiger willows were grown by inserting them into culture soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 without treatment of rooting promoter.

The inserts taken from the tigers were 10 ± 3.9 cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

<Example 2>

On March 30, 2010, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with IBA 100mg L ^-1 rooting promoter and then inserted on culture soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

<Example 3>

On March 30, 2009, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with an IBA 250mg L ^-1 rooting promoter and then inserted on culture soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

<Example 4>

On March 30, 2009, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with an IBA 500 mg L ^-1 rooting promoter and then inserted on cultured soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers including 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

<Example 5>

On March 30, 2009, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with IBA 1000mg L ^-1 rooting promoter and then inserted on culture soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

<Example 6>

On March 30, 2009, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with IBA 2000mg L ^-1 rooting promoter and then inserted on cultured soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

&Lt; Example 7 >

On March 30, 2009, the incision was taken from the tigers managed by the Forest Resources Development Department of the National Forest Research Institute.

The inserts were treated with IBA 3000mg L ^-1 rooting promoter and then inserted on culture soil mixed with sand: pitmos: vermiculite: pearlite in a volume ratio of 1: 1: 1: 1 on the same day to grow the hoverers.

The inserts taken from the tigers were 6.3 ± 0.9cm in length and used to shoot 1 year old tigers with 2 to 4 leaf eyes.

In the above treatment of the cuttings taken from the horns with rooting accelerator, 1 cm of the base of the insertions taken from the horns was immersed in the rooting promoter for 1 minute, 1 hour, and 6 hours.

The rooting result was confirmed 60 days after inserting the cuttings taken from the tigers in the culture soil.

The present invention has been described above in connection with specific embodiments of the present invention, but this is only an example and the present invention is not limited thereto. Those skilled in the art can change or modify the described embodiments without departing from the scope of the present invention, and such changes or modifications are within the scope of the present invention. In addition, the materials of each component described herein can be readily selected and substituted for various materials known to those skilled in the art. Those skilled in the art will also appreciate that some of the components described herein can be omitted without degrading performance or adding components to improve performance. In addition, those skilled in the art may change the order of the method steps described herein depending on the process environment or equipment. Therefore, the scope of the present invention should be determined by the appended claims and equivalents thereof, not by the embodiments described.

In the growth method of the hoverers using the cutting of the present invention, the timing of cutting and the cutting of the hoverers is clearly defined, and the callus formation rate is higher and the rooting is promoted compared to the untreated (control) without the rooting treatment agent in the cuttings at the time of the cutting. Determination of the concentration and treatment time of the rooting promoter can be provided to provide an optimal method for growing the hobber using cutting.

In particular, since the method of growing the horns using the cutting of the present invention uses the cutting, which is an asexual breeding method, the traits of the same parameters can be maintained in the tigers as it is, and stable seed production through the production of useful materials and bio-energy production forest composition It can lead to solving the problem of sexual growth. In addition, the production time of seedlings can be shortened and mass production can be performed at a low cost, which can greatly contribute to the hover-related industries, and thus the industrial availability is excellent.

Claims (9)

Tiger willow (Salix extracting the insert from the caprea L. ); Treating a rooting promoter with the collected insert; Inserting the rooting agent-treated insert into cultured soil; Hollow willow growth method using a cutting comprising a. The method of claim 1, wherein the harvesting time of the cutting is a 28-April 14 hobbling asexual growth method using the cutting. The method of claim 1, wherein the rooting promoter is IBA (indole-3-butyric acid) hobberry asexual growth method using a cutting. The method of claim 3, wherein the concentration of the IBA is 90 ~ 110mgL ^{- 1} hobberry asexual growth method using cutting. 4. The method of claim 3, wherein the IBA treatment method is a method for augmentation of the hobber using the cutting, characterized in that immersion in the rooting agent 0.5 ~ 1.5cm base taken from the hobber. 4. The method of claim 3, wherein the IBA treatment time is 50 to 70 minutes. The method of claim 1, wherein the insertion is 9.8 ± 2.1cm in length, 2-4 leaf leaves, 1 year old tiger willow shoots, characterized in that the auger growth method using a cutting. According to claim 1, The culture soil (sand): peat moss (peat moss): vermiculite (vermiculite): pearlite (pearlite) is a mixed soil characterized in that the mixed soil mixed in a volume ratio of 1: 1: 1: 1: How to grow holly. Claims proliferated by the method of any one of claims 1 to 8.

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