KR20120054297A - Oral composition containing extract of sapindus mukurossi gaertn - Google Patents

Abstract

PURPOSE: An oral composition containing sapindus mukurossi Gaertn extract is provided to prevent periodontal diseases and to remove bad breath. CONSTITUTION: An oral composition contains 0.0001-20 wt% of sapindus mukurossi Gaertn extract as an active ingredient. The composition further contains 30-60 wt% of sorbitol liquid and 1.0-3.0 wt% of carboxymethyl cellulose. The composition is used for preventing periodontal diseases or removing bad breath.

Description

Oral composition containing extract of sapindus mukurossi Gaertn

The present invention relates to a composition for oral cavity containing a patient-free extract, more specifically, containing a patient-free extract with excellent antimicrobial activity, and delivers the patient-free extract as a microbubble to the corners of the teeth to excellent gum disease prevention and bad breath removal effect It relates to a composition for.

In general, oral compositions designed to prevent gum disease were composed mainly of synthetic raw materials such as sodium chloride, allantoin chlorohydroxy aluminum, aminocaproic acid, tocopherol acetate. However, as interest in natural extracts has recently increased, studies on various plants have been actively conducted, and as the effects on oral cavity are known, many extracts have been applied to oral compositions related to gum disease prevention and bad breath control.

Korean Laid-Open Patent No. 2003-0000275 relates to a toothpaste manufacturing method using green tea extract, wherein the green tea extract has excellent antibacterial action and anti-cavity function due to high antibacterial activity of the catechin component, which is a main ingredient, and also has a bad breath in the mouth. It is disclosed that the effect as an odorant is excellent because it is excellent in removing.

Korean Laid-Open Patent No. 2002-0087672 contains a clove extract, which not only provides a refreshing feeling in the mouth, but also has an excellent effect in preventing bad breath and gum disease due to antibacterial activity, and has an analgesic effect that conventional cleaning agents do not have. It is disclosed that there is.

Korean Laid-Open Patent No. 2002-0066042 relates to a composition for promoting oral hygiene containing a pomegranate extract, and confirmed that pomegranate extract has an excellent effect of inhibiting the generation of free radicals and nitric oxide, thereby promoting oral hygiene through such antioxidant effects. It is disclosed that the role is excellent.

Korean Laid-Open Patent No. 2001-0036152 relates to a method for producing a plant extract powder and a composition for oral cavity containing the plant extract powder prepared by the method, which is a medicinal plant extract that is more widely known in folks than artificial antibiotics and fungicides, In particular, it is disclosed that the use of pine needles can provide excellent periodontal disease and caries prevention effect in the oral cavity.

As discussed above, many plant extracts have been used for the purpose of promoting oral hygiene, but in most cases have poor antimicrobial effects or simply through abstract efficacy such as anti-inflammatory, convergence, hemostasis, and blood circulation. It only shows the degree of preventing the occurrence of periodontal disease, there is a disadvantage in terms of stability was difficult to use as a composition for oral cavity.

Therefore, the present inventors investigated and analyzed various plant extracts for the prevention of gum disease and elimination of bad breath, and when the disease-free extract with excellent antimicrobial power is delivered to the corners of the teeth through microbubbles, it is excellent in preventing gum disease and removing bad breath. And the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition for oral cavity which exhibits excellent gum disease prevention and bad breath removal effect while being stable in the composition for oral cavity.

In order to achieve the above object, the present invention provides a composition for oral cavity containing the patient-free extract as an active ingredient.

The composition for oral cavity according to the present invention reduces the number of bacteria in saliva and effectively prevents gum disease such as gingivitis and periodontitis by delivering the patient-free extract having excellent antimicrobial power to the corners of the teeth with fine bubbles, and lowers the bad breath concentration index. Was excellent.

The present invention provides a composition for oral cavity containing sapindus mukurossi Gaertn extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The patient (sapindus mukurossi Gaertn) used as an active ingredient in the present invention is classified as a disease-free family, and since the bark of the fruitless fruit contains a large amount of saponin, the bark of the fruit has been used as a substitute for soap, bark Has been used as an expectorant. In recent years, various effects of patients have been reported, especially antimicrobial activity and lipase activity inhibitory activity has been reported.

The patient-free extract of the present invention can be obtained according to a conventional manufacturing method in the art, for example, after extracting and filtering the patient-free fruit with 80% ethanol solution, the filtrate is aged and filtered for 2 to 5 days and depressurized It can be prepared by concentrating and drying in a state.

The patient-free extract of the present invention is contained in 0.0001 to 20% by weight, more preferably 0.1 to 5% by weight based on the total weight of the composition. If the amount is less than 0.0001% by weight, the effect is insignificant, and if it is more than 20% by weight, oral irritation may occur, and the effect is also deteriorated, which is not preferable.

The composition for oral cavity of this invention may further contain sorbitol liquid and sodium carboxymethylcellulose. Sorbitol solution and sodium carboxymethylcellulose are used to form microbubbles and deliver the patient-free extract to the corner of the oral cavity.

The sorbitol solution of the present invention is contained in 30 to 60% by weight based on the total weight of the composition. This is not preferable because bubbles are less dense if less than 30% by weight, and toothpaste production is not easy if more than 60% by weight.

The carboxymethylcellulose sodium of the present invention is contained in 1.0 to 3.0% by weight based on the total weight of the composition. This is not preferable because bubbles are less dense if less than 1.0% by weight, and toothpaste is not easy to exceed 3.0% by weight.

In the present invention, the fine bubbles formed by the sorbitol solution and the carboxymethyl cellulose sodium have an average bubble size of 0.0001 to 0.5 mm, most preferably 0.1 mm. This is not preferable because less than 0.0001mm may cause irritation, and if it is more than 0.5mm, it is difficult to deliver the active ingredient in the corner of the tooth.

The composition for oral cavity of the present invention contains a patient-free extract, sorbitol solution and sodium carboxymethyl cellulose are very effective in preventing gum disease and removing bad breath.

The composition for oral cavity of this invention is not specifically limited in the formulation, For example, it may have formulations, such as a toothpaste, a mouthwash, and a tooth whitening agent.

The composition for oral cavity of the present invention may contain abrasives, wetting agents, auxiliary foaming agents, binders, sweeteners, pH adjusting agents, preservatives, active ingredients, fragrances, brighteners, pigments, solvents and the like which are commonly used according to the formulation and purpose of use. .

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited thereto.

Preparation Example 1 Preparation of Patient-Free Extract

The fruit of the patient tree was extracted with 80% ethanol solution and filtered, and then aged for 5 days. The obtained mature was filtered to remove impurities, concentrated and dried under reduced pressure to obtain a non-patient extract.

Example 1 and Comparative Examples 1 to 5

Toothpaste of Example 1 and Comparative Examples 1 to 5 was prepared according to a conventional manufacturing method in the composition of Table 1 below (unit: wt%). Using the non-patient extract obtained in Preparation Example 1, the sorbitol solution (70% solid component, liquid raw material) was purchased from Baekwang Industrial Co., Ltd., and sodium carboxymethylcellulose was purchased from Hercules.

Ingredients Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Sodium fluoride 0.30 0.30 0.30 0.30 0.30 0.30 Silica 17.0 17.0 17.0 17.0 17.0 17.0 Patient-free extract 1.0 - - 1.0 20.0 30.0 Sorbitol solution 50.0 50.0 25.0 25.0 25.0 25.0 Carboxymethyl Cellulose Sodium 2.0 2.0 0.7 0.7 0.7 0.7 Zaccarin Sodium 0.1 0.1 0.1 0.1 0.1 0.1 Methyl paraoxybenzoate 0.2 0.2 0.2 0.2 0.2 0.2 Spices 1.0 1.0 1.0 1.0 1.0 1.0 Sodium Lauryl Sulfate 2.0 2.0 2.0 2.0 2.0 2.0 Purified water To 100 To 100 To 100 To 100 To 100 To 100

Test Example 1 Bubble Size Comparison

Each toothpaste of Example 1 and Comparative Examples 1 to 3 was taken 3.5g and then brushed with a brushing machine (custom) for 30 minutes. The generated bubbles were placed on a slide glass, covered with a cover glass, and the average bubble size was measured using an optical microscope (Olympus DP70, using 4x lens). The measured bubble sizes are shown in Table 2 below.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Average bubble size (diameter, mm) 0.1 0.1 1.1 1.0

As can be seen from the results of Table 2, Example 1 and Comparative Example 1 containing a carboxymethyl cellulose sodium and sorbitol liquid in a preferred content range produced a fine bubble of 0.1mm on average, but the carboxymethyl cellulose sodium and sorbitol liquid It was confirmed that Comparative Examples 2 to 3 containing out of the preferred content range produces bubbles having a diameter of 1.0 mm or more.

Test Example 2 Measurement of the Number of Bacteria in Saliva

Sixty six subjects were suspended from oral environment management for 6 hours, and then the number of bacteria in saliva was measured by the following method. Since the average saliva bacteria in saliva was divided into six groups. For each group, oral treatment that could affect the experiment was forbidden, and it was allowed to brush with the test nutrient solution of Example 1 and Comparative Examples 1 to 5 for 2 weeks. After 2 weeks, all oral environmental management was stopped for 6 hours, and again the number of bacteria in saliva was measured.

<Method of Measuring Bacteria in Saliva>

60 ml of 5% aliquots of sterile 0.1% peptone solution (1 g of peptone was added to 1000 ml of 0.9% NaCl) were prepared in a 15 ml Falcon tube, and 60 peptone solutions were prepared. After 15 seconds of hydration, the peptone solution was diluted 10-fold with sterile 0.067 M Phosphate buffer solution (pH 7.2). 100 μl of this diluted solution is taken and dispensed into Mitis Salivarius medium (Agar) containing bacitracin and valinomycin antibiotics, and sterilized bending glass. The plate was plated as a whole, incubated for 12 hours in a 37 ° C. incubator, and then incubated for 48 hours at 37 ° C. in an anaerobic chamber. Then, the Streptococcus mutans (Colony counter) Colonies of Streptococcus mutans) were measured and recorded. The microorganisms of Streptococcus mutans colonies were identified by microscopic examination. After incubating the standard strain Streptococcus mutans NTCC 10449 in MSA at the same time and conditions, the colonies were identified. Only non-tans colonies were selected and measured. For each test subject, diluted peptone poloxes were dispensed into two plates of Mytis salivarius medium containing bacitracin and valinomycin antibiotics, and colony measurements were performed in two rounds of primary and secondary. The average value of the measurement was taken as the number of Streptococcus mutans of the subject. The measured results are shown in Table 3 below.

Elapsed time Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Right after the experiment 110 108 108 109 109 112 after 2 weeks 13 90 98 53 49 60

As can be seen from the results of Table 3, the composition of Comparative Examples 1 to 2 containing no patient extract, while the number of Streptococcus mutans hardly decreased, the comparative example containing 1, 20, 30% by weight of patient-free extract As for 3-5, bacteria level decreased compared with Comparative Examples 1-2. However, Example 1 containing the non-patient extract and at the same time generating microbubbles showed the most excellent antimicrobial effect as compared to Comparative Examples 3 to 5 that do not generate microbubbles.

Through this, it was confirmed that the microbubble well-delivered the patient-free extract to the minute corners of the teeth to maximize the antibacterial effect to significantly reduce the number of bacteria.

Test Example 3 Measurement of Bad Breath Removal Effect

120 subjects were suspended for 48 hours after oral administration of all oral environment, and the degree of bad breath was measured using a harimeta. Each group was made to brush with the test fern of Example 1 and Comparative Examples 1-5. At 30 minutes after brushing, the degree of bad breath was measured again. Each test fern was then used twice a day for 28 days. After 7 days, 14 days, 21 days and 28 days elapsed, the degree of bad breath was measured again 30 minutes after brushing the teeth, and the degree of bad breath according to the use period of the toothpaste composition is shown in Table 4 below.

Period of use Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 30 minutes 215 219 223 220 220 220 7 days 130 217 222 170 150 180 14 days 105 215 217 145 130 160 21st 86 212 219 115 103 140 28 days 62 209 217 102 96 131

Looking at the results of Table 4, while the bad breath score of the oral composition of Comparative Examples 1 to 2 without using the patient-free extract is almost unchanged, Comparative Examples 3 to 5 containing the patient-free extract 1, 20, 30% by weight Compared with Comparative Examples 1 and 2, the bad breath score was somewhat reduced. However, Example 1 containing 1% by weight of the patient-free extract and at the same time generating microbubbles exhibited the most excellent bad breath removing effect compared to Comparative Examples 3 to 5 which do not generate microbubbles.

Through this, it was confirmed that the microbubble delivers the patient-free extract well to the minute corners of the teeth to maximize the bad breath removal effect.

[Test Example 4] Measurement of gum disease prevention effect

To determine the effect of preventing gum disease, the gingival index was measured and the method was as follows. First of all, 80 subjects were screened and oral administration was prohibited during the experimental period. 20 subjects were divided into Example 1 and Comparative Examples 1 to 3, and the gingivitis index was measured according to the gingivitis grading criteria of Loe modified by Talbott, Mandel and Chilton.

Loe's Gingivitis Index Modified by Talbott, Mandel and Chilton

Loe's Gingivitis Score Standard

0: no inflammation

1: Mild gingivitis (weak change of color and weak edema)-no bleeding during periodontal cyst measurement

2: moderate gingivitis (redness, edema)-bleeding during periodontal cystic measurement

3: highly gingivitis (ulcer)-tendency to bleed on its own

Elapsed time Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Initial value 1.61 ± 0.21 1.60 ± 0.21 1.60 ± 0.21 1.58 ± 0.21 2 weeks 1.26 ± 0.14 1.59 ± 0.24 1.60 ± 0.25 1.42 ± 0.22 4 weeks 1.07 ± 0.16 1.58 ± 0.20 1.58 ± 0.22 1.25 ± 0.18 Improvement index 0.54 ± 0.18 0.02 ± 0.22 0.02 ± 0.23 0.33 ± 0.20

Looking at the results of Table 5, Comparative Examples 1 and 2 containing no patient extract had almost no gingivitis alleviating effect, while Comparative Example 3 containing 1% by weight of no patient extract showed some degree of gingivitis alleviating effect. However, in the case of Example 1 containing 1% by weight of the patient-free extract to generate microbubbles showed an excellent gingivitis improvement effect compared to Comparative Example 3 that does not generate microbubbles.

Through this, it was confirmed that the microbubble effectively delivers the patient-free extract to the minute corners of the teeth, effectively preventing and improving gum disease such as gingivitis.

Claims (7)

Oral patient (sapindus mukurossi Gaertn) composition containing an extract as an active ingredient. The composition for oral cavity of claim 1, wherein the patient-free extract is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. The oral composition according to claim 1, wherein the composition further contains a sorbitol solution and sodium carboxymethylcellulose. 4. The composition for oral cavity according to claim 3, wherein the sorbitol solution and sodium carboxymethylcellulose generate fine bubbles having an average bubble size of 0.0001 to 0.5 mm. 4. The composition for oral cavity of claim 3, wherein the sorbitol solution is contained in an amount of 30 to 60 wt% based on the total weight of the composition. 4. The composition for oral cavity of claim 3, wherein the carboxymethylcellulose sodium is contained in an amount of 1.0 to 3.0% by weight based on the total weight of the composition. The composition for oral cavity according to any one of claims 1 to 6, wherein the composition is for preventing gum disease and removing bad breath.