KR20120043793A - Snp for diagnosing hip dysplasia in dog and uses thereof - Google Patents

Abstract

PURPOSE: A marker for early diagnosing hip joint dysplasia in a dog is provided to specially care a dog with risk of hip joint dysplasia and to predict genetic information. CONSTITUTION: An SNP for early diagnosing hip joint dysplasia in a dog contains one or more polynucleotides selected from polynucleotides containing 10 or more bases containing each SNP base or complementary polynucleotide thereof. A microarray for early diagnosing dysplasia contains the SNP polynucleotide, polypeptide encoded by the polynucleotide, or cDNA thereof.

Description

SNP for early diagnosis of canine hip dysplasia and its use {SNP for diagnosing hip dysplasia in dog and uses e}

The present invention relates to an SNP for early diagnosis of canine hip dysplasia and its use. More specifically, the present invention relates to a SNP (single nucleotide polymorphism) position base in a polynucleotide consisting of SEQ ID NOs: 1 to 25. Canine hip dysplasia comprising SNP (single nucleotide polymorphism) for early diagnosis of canine hip dysplasia consisting of one or more polynucleotides selected from polynucleotides or complementary polynucleotides thereof. An early diagnosis microarray, a canine hip dysplasia early diagnosis kit including the microarray, and a method for early diagnosis of canine hip dysplasia using the SNP position base.

The genomes of all organisms undergo spontaneous mutations that produce variants in the progeny sequence during evolution and polymorphisms occur (Gusella, Ann. Rev. Biochem. 55, 831-854, 1986). Restriction fragment length polymorphism (RFLP), short tandem repeats (STR), and single nucleotide polymorphism (SNP) are known as the polymorph. The SNP is known to occur once in about 1,000 bp in humans. If these SNPs affect a phenotype such as a disease, the polynucleotides containing the SNPs can be used to diagnose this disease. Monoclonal antibodies that specifically bind to the SNPs can also be used for the diagnosis of diseases.

There are about 400 breeds of dogs worldwide, which is the result of excessive breeding of humans. As a result, dogs have high inbreeding rates and are exposed to various genetic diseases. Genetic disorders in dogs include hip dysplasia, obesity, cataracts, degenerative retinal atrophy, allergies, epilepsy and kidney disease. DNA tests for genetic diseases provide important information to eliminate key risk variants. Genetic disorders in dogs that are currently being used for DNA testing include dwarfism (Germany), degenerative retinal atrophy (mastiff), and malignant fever (all breeds), and are being developed continuously through molecular biological techniques.

In 2004, draft dog genomes were deciphered, increasing the availability of genomic information on disease, body type, and behavior. The unique DNA information of a dog can be used for selection by genetic information while being able to represent specific expression traits. Molecular markers are genetic markers that are very useful for improving selection accuracy and for early diagnosis of qualitative traits such as diseases. Currently, some companies serving dog DNA testing are using the commercially available molecular markers (SNPs) in genes related to genetic disorders such as degenerative retinopathy, dwarfism and epilepsy in the dog industry.

Dogs are important animal resources in modern society that enrich human emotions as pets. The domestic pet market is estimated at about 1 trillion won, and the production scale of the disease diagnosis kit for pets is about 700 million won in 2007, which is lower than that of foreign markets, and there are no domestic DNA test services. However, considering the national interest in pets and the steady expansion of the market, continued growth is expected.

An object of the present invention is to develop a genetic marker that can maximize the improvement of genetic ability while improving the accuracy in selecting excellent dogs without genetic diseases and eliminating risk factors such as genetic diseases.

Korean Patent No. 10-0754398 discloses multiple SNPs for diagnosing cardiovascular disease, and Korean Patent No. 10-0933065 discloses a set of SNP genes for diagnosing aspirin-sensitive asthma.

The present invention is derived from the above-mentioned needs. Since hip dysplasia of dogs is known to be a result of several genetic factors, it is necessary to analyze genetic variations of all 78 chromosomes to develop genetic markers for early diagnosis of hip dysplasia. The present invention was completed by discovering a single nucleotide polymorphism (SNP) using a dog 22K SNP chip, and developing a genetic marker that can detect and predict early hip dysplasia and a significant single nucleotide polymorphism.

In order to solve the above problems, the present invention is one or more polynucleotides selected from polynucleotides consisting of 10 or more consecutive bases each containing a single nucleotide polymorphism (SNP) position base in the polynucleotide consisting of SEQ ID NO: 1 to 25 Or it provides a single nucleotide polymorphism (SNP) for early diagnosis of canine hip dysplasia consisting of a complementary polynucleotide thereof.

The present invention also provides a microarray for early diagnosis of canine hip dysplasia comprising a polynucleotide of the SNP, a polypeptide encoded therein or a cDNA thereof.

The present invention also provides a kit for early diagnosis of hip dysplasia of the dog comprising the microarray.

The present invention also provides a method for early diagnosis of canine hip dysplasia using the SNP position base.

By using the SNP for early diagnosis of canine hip dysplasia of the present invention, it is possible to prepare special care for dogs at risk of hip dysplasia, which is a genetically advanced disease, and to predict important genetic information when breeding for breeding. Therefore, it may be useful to select excellent dogs without hip dysplasia.

1 shows the location of SNPs associated with hip dysplasia on dogs 4, 38 and X chromosome.

In order to achieve the object of the present invention, the present invention is a polynucleotide consisting of SEQ ID NO: 1 to 25 each SNP (single nucleotide polymorphism) position (123 for the SEQ ID NO: 1, 120 for the SEQ ID NO: 2, SEQ ID NO: 2) 119th for 3, 122th for SEQ ID NO. 4, 119th for SEQ ID NO. 5, 122th for SEQ ID NO. 6, 87th for SEQ ID NO. 7, 124th for SEQ ID NO. 8, SEQ ID NO. In case 121, in case of SEQ ID NO: 10, in case of SEQ ID NO: 10, in case of SEQ ID NO: 10, in case of SEQ ID NO: 10 The 131th time for SEQ ID NO: 16, the 118th time for SEQ ID NO: 17, the 181th time for SEQ ID NO: 18, the 120th time for SEQ ID NO: 19, the 120th time for SEQ ID NO: 20, the 108th time for SEQ ID NO: 21, 147th SEQ ID NO: 22, sequence One or more polynucleotides or complementary polynucleotides thereof selected from the group consisting of ten or more consecutive bases comprising a base 119 for No. 23, 120 for SEQ ID No. 24 and 122 for No. 25) It provides SNP (single nucleotide polymorphism) for early diagnosis of canine hip dysplasia.

SEQ ID NOs: 1 to 25 are polynucleotides containing the nucleotide sequence of each of the SNP (single nucleotide polymorphism) sites. SEQ ID NOs: 1 to 25 are polymorphic sequences including polymorphic sites. A polymorphic sequence refers to a sequence comprising a polymorphic site representing SNP in a polynucleotide sequence. The polynucleotide sequence may be DNA or RNA.

The polynucleotide of each single SNP constituting the SNP for early diagnosis of canine hip dysplasia according to the present invention is preferably 10 or more consecutive bases, more preferably 10 to 100 consecutive bases.

The present invention also provides a microarray for early diagnosis of canine hip dysplasia comprising a polynucleotide of the SNP, a polypeptide encoded by the same or a cDNA thereof. Preferably, the polynucleotide may be immobilized on a substrate coated with an active group of amino-silane, poly-L-lysine or aldehyde, but is not limited thereto. Preferably, the substrate may be, but is not limited to, a silicon wafer, glass, quartz, metal or plastic. As a method of immobilizing the polynucleotide on a substrate, a micropipetting method using a piezoelectric method, a method using a pin-shaped spotter, or the like can be used.

Microarrays according to the invention can be prepared by conventional methods known to those skilled in the art using the polynucleotides or their complementary polynucleotides according to the invention, the polypeptides encoded by them or the cDNAs thereof.

The present invention also provides a kit for early diagnosis of canine hip dysplasia comprising the microarray.

The kit according to the present invention may further include, but is not limited to, a primer set used to separate and amplify DNA including the SNP from the subject in addition to the microarray of the present invention. Such suitable primer sets will be readily apparent to those skilled in the art with reference to the sequences of the present invention.

The present invention also provides a step of separating a nucleic acid sample from a subject and

Provided is a method for early diagnosis of canine hip dysplasia comprising determining the genotype of a polymorphic site that is each SNP position base of at least one polynucleotide selected from SEQ ID NOs: 1-25. Preferably, the subject is a dog.

In the method of the present invention, the method of separating DNA from a subject may be performed through conventional methods known in the art. For example, it can be done by directly purifying DNA from tissue or cells or by specifically amplifying and isolating specific regions using amplification methods such as PCR. In the present invention, DNA includes not only DNA but also cDNA synthesized from mRNA. Obtaining nucleic acids from a subject may include, for example, PCR amplification, ligase chain reaction (LCR) (Wu and Wallace, Genomics 4, 560 (1989), Landegren et al., Science 241, 1077 (1988)), transcription amplification. transcription amplification (Kwoh et al., Proc. Natl. Acad. Sci. USA 86, 1173 (1989)), self-sustaining sequence replication (Guatelli et al., Proc. Natl. Acad. Sci. USA 87, 1874 (1990)), and Nucleic acid based sequence amplification (NASBA) may be used, but is not limited thereto.

Determination of the nucleotide sequence of the isolated DNA can be made by various methods known in the art. For example, by determining the nucleotide sequence of a nucleic acid directly by the dideoxy method, or by hybridizing the probe comprising the sequence of the SNP site or a probe thereof to the DNA and measuring the degree of hybridization obtained therefrom. A method of determining the nucleotide sequence may be used, but is not limited thereto. The degree of hybridization may be achieved by, for example, labeling a detectable label on a target DNA to specifically detect only the hybridized target DNA, and other electrical signal detection methods may be used, but is not limited thereto. The method for early diagnosis of canine hip dysplasia according to the present invention preferably hybridizes a nucleic acid sample isolated from the subject with a polynucleotide comprising a SNP according to the present invention or a complementary polynucleotide thereof, or a polynucleotide hybridizing thereto. And then detecting the hybridization result.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example  One: DNA  Sample Preparation and Diagnosis of Hip Dysplasia SNP Screening

DNA was extracted from the blood of 24 patients with hip dysplasia with 24 hips and 24 normal patients with Labrador retriever, which was diagnosed with hip X-ray, using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). SNP genotyping was performed using Canine SNP20 Beadchip (Illumina, San Diego, CA, USA), which consists of 22,362 SNP information.

All SNP data obtained after genotyping were chi-squared test of the R / SNPassoc package for Hardy-Weinberg equilibrium (HWE) and minor allele frequency (MAF). The results that did not meet certain conditions were excluded (HWE; P <0.001, MAF; <1%). For the detection of hip phenotype-related quantitative trait loci (QTL), we used SNP marker regression (R-Development Core Team) to test the association between hip phenotype and SNP markers. The additive effect of SNP markers was analyzed as follows.

y = μ + Sex _i + SNP _j + e _ij

y: hip phenotype

μ: overall mean

Sex _i : Genotype effects (i: female, male and castrated male)

SNP _j : genotype effect (j: AA, AB and BB)

e _ij : random error, N ~ (0, σ ² _e )

To perform false positives due to randomly generated errors, such as problems in multiple testing, such as genome wide association tests, 10,000 permutation tests were performed to achieve a genome of 0.1%. Genome-wise P-values were calculated (FIG. 1 and Table 5).

As shown in Tables 1 to 3, out of a total of 22,362 SNPs on the dog genome, 25 SNPs having significant significance for hip dysplasia in 11, 4, and 10 chromosomes 4, 38, and X were found, respectively. Genome-wise P <0.001 was used, and this genotype could be used to predict the probability of hip dysplasia in dogs.

Of the bases of the SNP positions shown in Tables 1 to 3 above, the bases of the normal group and the risk group are summarized in Table 4 below.

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> SNP for diagnosing hip dysplasia in dog and uses <130> PN10191 <160> 25 <170> KopatentIn 1.71 <210> 1 <211> 212 <212> DNA (213) Canis familiaris <400> 1 aaagatccct gtagtccagg gtcactgcaa ctgcaacaga caggcttggg acagacatct 60 gatctgacta cagtcctcac ccactaacaa aatcctctca gggattttgg agcacaggaa 120 gagtgctcca cagtcctgga aaatgagcaa agagtatcta gtctgaccta actacaagcc 180 tcaggtggct ctgattagct tcttaacaaa ac 212 <210> 2 <211> 267 <212> DNA (213) Canis familiaris <400> 2 tttagtggct ctgaacaacc taccatggct ttcgaagaaa atcagctgtg gtcccaagga 60 catttgaaac ttgggcaaag taccaagaaa aggcttatga gggaggcatt gttttggggc 120 gtcttatagc ttggaatcat tatctgcgaa tgaaagttag aagaaagatt tcagcttatt 180 taaagaactt tccattagtc aaaaccatct atagatgaaa caaacacttt tttataaaag 240 aatgaatttc tcattcttta gggcatt 267 <210> 3 <211> 235 <212> DNA (213) Canis familiaris <400> 3 gcagaagttt ctgtgtaagc gccaatccca gttcctgttt atcaccctgc cctagcttat 60 tcgttgttcc ccttacttgc agccgcttta cggtgtaata cgtgtctggc tgcctgtccg 120 tgcagccacc tgaatgtctc tttttggaga aagacagggg tagaagctct gtagcaactg 180 aactctttaa agatttgtat ttatttgaaa gagacagaga gagagagggg cgggg 235 <210> 4 <211> 211 <212> DNA (213) Canis familiaris <400> 4 aggccaggaa tggaagccag tgggtggtga gcatatggga aacagagggc ccacattctc 60 agaagagtga gggagctggg gtgggagtga cagttcaggg tgctgccacg ggaaacagtg 120 gggccatgat gggattctgc tggcccgttg ggagagggtc acctgggtta attaaaaggg 180 tgtccgagtc ctgggaagct tcctccctcc c 211 <210> 5 <211> 257 <212> DNA (213) Canis familiaris <400> 5 ttgttagggg ttccaggtac tcctaggaga aactgccaac tctatgttga gatgaaacta 60 gcacaaccct ggcagggaga tgcagggtgc caaccaaccc ctccacagta tagtttaaca 120 gagaaagctg tggctccagg aaaggaaagg ggtgacccct taccacctat tgtgctgtag 180 ggccctagcc cttatctgct ctcttccagg agcatctgct agaacaaaca gaaactcctc 240 tccccgtatc ctcctgt 257 <210> 6 <211> 262 <212> DNA (213) Canis familiaris <400> 6 gaaagttcag gcttttgtgg gaaagaaacc agatcaacaa catggacttt agggaggtga 60 gaaaataacc actctgggta gtaatatccc ctttggacaa actagactga gagaagccca 120 gggaagtgtt agaatagttt tcagccttga ctggatcaga atcacttgtg gatttttcta 180 aatattttat tcattcatct ctctcacaca cacacaggca gagacataag cagagggaga 240 agcaggctcc ccatggaact tg 262 <210> 7 <211> 211 <212> DNA (213) Canis familiaris <400> 7 cccttatccc catcccatcc agccacatcc aaatgatttt aatatattgt ttaggttaaa 60 ccatccagtt aaaggctaat gcagatctgg gaaggatcag gaaacaagag aaagtgaagg 120 agtgccaagg aaagagttgg tggaagtctg gaggggaaat tagttataaa tgtcaagttg 180 gaagtctaga ggagataaaa aggcagagtg t 211 <210> 8 <211> 216 <212> DNA (213) Canis familiaris <400> 8 gatcagttct catctccttc tttaggtatc taggagatct tgggatgcca atcaggtgtt 60 tgtatctgac tcaacctttc tatgctttac tccccagtat gtgaaatcgt tttcataagg 120 agaggaactt tctgtgggcc acataaacaa agaaacccag aaacagggtt cagcctggag 180 aggagagatt atgattgggg tatcaggact cagggg 216 <210> 9 <211> 211 <212> DNA (213) Canis familiaris <400> 9 ttggtctacc agcagatgac agcggacagg agcccctagg gctggggttg gaggcactgc 60 ccgccaagga gccaccagga cagcttcact gaggggaaca cacgcaggac aggagcagag 120 cggagtgggt atcaccccag ctggagacgc cacctcacac gacggcctct ttcctgagtt 180 tatctcccct ttactcgcag atgagagcaa g 211 <210> 10 <211> 218 <212> DNA (213) Canis familiaris <400> 10 tcctttggct agttcccacg tgccttgtgt ttttccatct tcctgatcac ggccgtagct 60 gcagtcttca aacctgctca gaggccactg cccaccttgt ctctaccctg ctctcatcgc 120 attggggtac tctcgtggga ggtgctgagg gcttctgaag acccctaggg aggcttcctt 180 gtaaactgaa gaggagacct ttaatcattt cgacctcc 218 <210> 11 <211> 223 <212> DNA (213) Canis familiaris <400> 11 tggtttagat tctttatttg ttttatttta atgaccttct gaatcattca tttttctact 60 tctgaacaga ttcacttttg gccacttcct tgtatagaaa tccaaacctc cctttaaaac 120 tcagcaccaa caaccccctt ctcccaaaaa ttctcatcta taccattccc tcttcaggaa 180 gaagtattcc cctccccact ctttgtacca ttcatctggt att 223 <210> 12 <211> 280 <212> DNA (213) Canis familiaris <400> 12 gcccagggca tgatcctgga gtcccaaaat taagtcccac attgggctcc ctgtgtggag 60 cctgcttctc cctctgcctc tctctctctc tctctctctc tctctttctc tcactctctg 120 tgtctctcat gaataaataa aaaaaaatcc ttaaaaaaag aaaagaaaaa tatcttttac 180 attgttcgaa gaccactaat tttaaaagaa taacattaga gaaaatgcta cttggctttt 240 ctttgatgat atttcccctc catgttataa ttttgaatgc 280 <210> 13 <211> 219 <212> DNA (213) Canis familiaris <400> 13 gctgtttgca cataactatt ttagcatcct gtttctatct tagagatatc ccagattgga 60 aaataatggt cagtaaattg agaaggaaaa aaatggcagc ccacacacac tcggatctgt 120 ccgctcatga gtgtttcatg cattcctctc atcttatttc ttgtcctctg tcctccaaga 180 aaaatagttg tctctgttgg ctacactaga cccttgtgc 219 <210> 14 <211> 214 <212> DNA (213) Canis familiaris <400> 14 aacttcttca gcttcgggga gatgttaaat tccaagactc cttcactatc ctaaagaaac 60 agcaataaaa tgtgtttcag gattcaattc cttcagcttc agaagctgag acggatgaac 120 tagtttgggg gaaatgagac atgacccaac ccttcaccta aacaaaaatg actccaaatt 180 gaacttttcg caaagttcac catcctatgt gata 214 <210> 15 <211> 252 <212> DNA (213) Canis familiaris <400> 15 ccctgtggga tggagtccca catcgggttc cgcgcaggga gcctgcttcc ccctctgcct 60 gtgtctctca tgaataaata aatgaaatct taaaaaaaaa aaagaaaata gatggtaaaa 120 tattgtacac aatggccatg tgggcgctgg ttgttgatag tccatgaact tgacgccttc 180 tgggagaagg agaagcctgg atacgcttct ctctgccctc tgaagctgca ttaaataaaa 240 cccagctcag at 252 <210> 16 <211> 245 <212> DNA (213) Canis familiaris <400> 16 ggctccatgc agggagcctg acatgggact cgatcctggg tctccagaat caggccctgg 60 gctgaaggtg gcactaaacc actgagccac ccgggctgcc ctggaggatt ttccagacca 120 ggagagaacc ggagcatgag tggtcctgtt gaccggcgaa ggagaagagg aggatttgat 180 ggtggaggca tgagcagagg tgggcagaga ggaggataca gtggaatggg cagcgctgga 240 gagcc 245 <210> 17 <211> 217 <212> DNA (213) Canis familiaris <400> 17 tttgctgtat gactcaactc tggacacctc tgtttaactc atgcatgtgc tgcttcttcc 60 taggatattc actggctcat ctggctttct caccctcaag taaaataaag tctcgtggcc 120 atgacttgct caacaatttt ccatgttagc caggtaatat agaggctcag aggtctctgc 180 actgacatca gctctacttc caggaatgaa aaagaaa 217 <210> 18 <211> 317 <212> DNA (213) Canis familiaris <400> 18 gcacacaggc ttacaatttt aataggtatt gccgtatcat tttcaagaga ggttatggtt 60 ataccagttt atacttttac caagagtact tgtttacctc acttttgtca acatttatca 120 tcaggcttga gtttttgcca gtgtgaatgg gtaaatggta gaagttcatt gttttagtgt 180 ctctcagatt gatagcattt tatatgttag gccttctctt ctgtgaattg cctatttgtg 240 ttcgttcttc attttcctat tggattctta gtcttggaac ccaacacaat aggaatattt 300 gttacagatc ttttctt 317 <210> 19 <211> 213 <212> DNA (213) Canis familiaris <400> 19 ggaaaatggt cagatctccc ctgatggctt cttgtcaaaa tctgctccac cagagcttat 60 gaatatggcg ggagatggca ttccccacaa ccaaatggat tctctgtcag atgacttcac 120 taccctaagg aaagatggcc tgattcccaa acctggtact aacacacttc taggaggaag 180 caaaaagtgc agtgtctctg tcgaggatca gaa 213 <210> 20 <211> 219 <212> DNA (213) Canis familiaris <400> 20 atctttccaa gtgtgtgacc ttcggtggct tctgccattc tagctgcaga gtccttctcg 60 tgttctgctt gccgaactct gtgttcccgt cattctgttc acatagccgc tgcctggcac 120 gggagcgcat cacttgcctt caagagcttt ggaagcggtc acttgtgctc cttgagcgtc 180 agctgttgta ggtggaccct tggcccctcc attgacctg 219 <210> 21 <211> 229 <212> DNA (213) Canis familiaris <400> 21 ttgggcatca ctgtcatgaa ctagaagaga gaaatgaata aatctatctc attattccat 60 ttagggctcc ctattgccaa cactgaagta aagtgatgct gacctgtgac cttttaattg 120 cttcccctag aatatatcag cccataaatt ttgtgaaaag gaagaaattg ccctgtagag 180 tgtctccttc agtggtgaaa tggcattcat agaggaggac tagcctgta 229 <210> 22 <211> 247 <212> DNA (213) Canis familiaris <400> 22 tctttgaact ccactttctg tactaataaa atgaagacaa taaatctatc ttgcagcatg 60 tttatgaaga ttaaatgtgc acattttcct aaagcatgga gaaactattt tctagatatg 120 ttaatgggta gttggtgcct atgtatgtga atcaggaaga gtaccatgat ctaaagattt 180 ctgaaaataa tgaagttatt gtatttgtag tcttacacac caacttctca gtctatcgtg 240 aagtcta 247 <210> 23 <211> 212 <212> DNA (213) Canis familiaris <400> 23 tggggtgggt acgttttaca aaaagaaaac aacaacaaca ccctcccccc aatcccaacc 60 ggttaaaaca agtaaaatgg cagatctcaa aaaacatttg ggaagcagtg gaccagtcgg 120 taaataccag aacaggcatt aaaataaccc aggtcaggta ccgagaggta taaattcttg 180 actcatcttc catggatctg tatttaaata ca 212 <210> 24 <211> 255 <212> DNA (213) Canis familiaris <400> 24 tcctgtgcag agggacacgt gaaggcccta ggtcctcctg ggagaaaaga tttccctcct 60 aatgatccag ggactaggat ttcattctga aatggaatgg agaatggcag gtgtggcttt 120 ctggggattg agaaccacag gccccaggag attcaggaaa ggacagtaat gccctgctgc 180 tcaggccccg gtggaggaat catatcctca gctcagcaac accacccgcc cccctacggc 240 cccgaggaaa ggcac 255 <210> 25 <211> 217 <212> DNA (213) Canis familiaris <400> 25 gcattgggaa ggtaaggtgg ggcagaggct ccaatttctt ttccatttcc tgtatattct 60 gcactgcttt cagtgtttta attgattaca tcaactgtgc tcatttccct ggacaaattg 120 gcctttacac agaagaggca attacctggg cttgttccat cccaatcagc agagcaggca 180 ggagcctctt tggggcccca caagattata ttatctg 217

Claims (7)

In the polynucleotide consisting of SEQ ID NO: 1 to 25, each SNP (single nucleotide polymorphism) position (123 for SEQ ID NO: 1, 120 for SEQ ID NO: 2, 119 for SEQ ID NO: 3, 122 for SEQ ID NO: 4) The 119th for SEQ ID NO: 5, the 122th for SEQ ID NO: 6, the 87th for SEQ ID NO: 7, the 124th for SEQ ID NO: 8, the 121st for SEQ ID NO: 9, the 119th for SEQ ID NO: 10, 120th for SEQ ID NO: 11, 190th for SEQ ID NO: 12, 122th for SEQ ID NO: 13, 120th for SEQ ID NO: 14, 145th for SEQ ID NO: 15, 131th for SEQ ID NO: 16, SEQ ID NO: 118th for 17, 181th for SEQ ID NO: 18, 120th for SEQ ID NO: 19, 120th for SEQ ID NO: 20, 108th for SEQ ID NO: 21, 147th for SEQ ID NO: 22, and 119th case, 120 for SEQ ID NO.24 And SEQ ID NO: 25 122-th) base dog hip dysplasia single nucleotide polymorphism (early diagnostic SNP consisting of one or more of the polynucleotide or a complementary polynucleotide selected from a polynucleotide consisting of at least 10 consecutive bases containing case). A microarray for early diagnosis of canine hip dysplasia, comprising the polynucleotide of the SNP of claim 1, a polypeptide encoded therein or a cDNA thereof. The microarray of claim 2, wherein the polynucleotide is immobilized on a substrate coated with an active group of amino-silane, poly-L-lysine, or an aldehyde. 4. The microarray of claim 3, wherein the substrate is a silicon wafer, glass, quartz, metal, or plastic. Kit for early diagnosis of hip dysplasia of the dog comprising the microarray of claim 2. Separating the nucleic acid sample from the subject, and
A method for early diagnosis of canine hip dysplasia comprising determining the genotype of a polymorphic site, each SNP position base of at least one polynucleotide selected from SEQ ID NOs: 1-25. The method of claim 6, wherein the subject is an individual.

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