KR20120010439A - An antibacterial and antifungal composition containing the extracts and fractions of Hosta capitata as active ingredient - Google Patents

Abstract

PURPOSE: A composition containing Hosta capitata extract or fraction thereof is provided to suppress growth of fungi and to ensure excellent antibacterial and antifungal activity. CONSTITUTION: An anti-fungal and antibacterial pharmaceutical composition for Hosta capitata extract as an active ingredient. The extract is isolated using water, low alcohol of C1-C4, or mixture thereof. An antifungal and antibacterial composition contains organic solvent fraction prepared by isolating the extract with organic solvent. The bacteria include C. albicans, S. cerevisiae, E. coli, B. subtilis, Propionibacterium acnes, Epidermophyton floccosum, Trichophyton ruburm, Staphylococcus aureus, Staphylococcus epidermis, Pityrosporum ovale, candida albicans, or Trichomonas Vaginalis.

Description

 Antibiotic and antifungal composition containing the extracts and fractions of Hosta capitata as active ingredient}

The present invention relates to a composition for antibacterial and antifungal, containing as an active ingredient walnut beetle extract or fractions thereof having antibacterial and antifungal activity against pathogenic bacteria, pathogenic fungi, floating fungi.

Antibiotics are substances that have a selective toxicity that kills microorganisms and are not toxic to humans or animals and are not inactivated by enzymes in the body. This is mainly due to the replication of DNA, transcription and decoding of genetic information, and electronic energy. It inhibits transport, biosynthesis of cell walls, and the like, through the mechanism of inhibiting the growth of microorganisms.

Antibiotics Fleming (Sir Alexandor Fleming) the first occurrence of penicillin in 1929, and Florisil (Flory), such as a penny room Solarium Cri scavenger titanium (Penicillium 1939 Chrysogenum ) has been known as a miracle medicine by first separating and purifying penicillin from cultures and saving the lives of many patients with infectious diseases. Since 1959, full-fledged research has developed benzylpenicillin, streptomycin, tetracycline, and macrolide erythromycin, which have been used as antibiotics to date. . Since penicillin (penicillin) was first developed by a synthetic method in 1965, semisynthetic penicillin, methicillin, and the like, and in 1973, cephazoline, cephazoline.

However, most antibiotics not only have several chiral centers, but also have complex structures such as macrolides or peptides, and thus, the synthesis thereof is difficult, and these synthesis methods also have low economical efficiency compared to fermentation methods. In addition, the recent strict safety review process is often found that they can not meet 100% of the safety of the human body, the safety of microbial disinfectant drugs is emerging as an urgent problem. In the case of microbial fungicides, there is a strong movement to avoid the use of synthetic fungicides and to use natural antimicrobial agents without contamination to the human body, and research and development on this are being actively conducted. The most active use of natural antimicrobial agents is the antimicrobial treatment of fiber, deodorant processing and antimicrobial processing of filters. Since fibers and fabrics are in direct contact with the skin, there is a risk that harmful processing agents may be absorbed into the human body through the skin. In the filter, the harmful processing agent contained in the air released or diffused from the processed filter in the process of circulating the air can be directly introduced into the human body through the respiratory system. As safety is emphasized, various types of safety evaluations such as transdermal toxicity, acute toxicity and mutagenicity are mandatory for antimicrobial agents. Among the air cleaning filters, the air cleaning filters for home use and the air conditioner filters for automobiles are circulating in a narrow space, and in particular, the air conditioner filters for automobiles are used only four to five months a year and are discontinued. Complete antimicrobial treatment is required because it cannot be used to dismantle and replace filters frequently. In addition, in the case of automotive filters, the stability of the antimicrobial agent may be considered more important given that the occupant is breathing in an extremely narrow space.

January bibichu (Hosta capitata ) is also known as lily and ivy, and is a lily and monocotyledonous plant that grows in limestone and is 35 to 65 cm in height. Stems grow straight, leaves are gathered from the roots, have a wide egg shape, 10 to 16 cm in length, and 5 to 7.7 cm in butterfly. It is distributed in Korea (Jeonnam Baekunsan, Jeonbuk Deokyusan, Gyeongnam Jirisan, and Gyeongbuk Gayasan) and Japan, and the root of the hibiscus is called Jaokjak-geun in Chinese medicine. It is known to be used to treat sore throat pain, toothache, stomach pain, blood shelf, crayfish, onggi, and nausea.

Therefore, the present inventors inhabit the harmful bacteria and fungi that we encounter in everyday life, for example, the causative agent of oral disease, dermatological fungi, air conditioner filter and the like to treat and prevent the infection of floating fungi that are infected through the air. As a result of continuing research to develop an antimicrobial agent having an effect, some of the extracts and their fractions of the Korean native plant, Ilwolbichu, have excellent antibacterial and antifungal activity, and can be used as an active ingredient of antibiotics because they have no side effects. The present invention was completed by confirming.

The purpose of the present invention is January shinju ( Hosta capitata ) to provide an antimicrobial and antifungal pharmaceutical composition containing the extract as an active ingredient.

It is another object of the present invention to provide an antimicrobial and antifungal pharmaceutical composition containing an organic solvent fraction prepared as an active ingredient by extracting an extract of the sun shin nabe as an organic solvent.

It is another object of the present invention to provide an antibacterial and antifungal skin external preparation containing an extract of January shinjube or fractions thereof as an active ingredient.

It is another object of the present invention to provide an antibacterial and antifungal cosmetic composition containing an extract of January shinjube or fractions thereof as an active ingredient.

It is another object of the present invention to provide an oral antimicrobial composition containing an extract of January shinju or a fraction thereof as an active ingredient.

Another object of the present invention is to provide an antimicrobial filter coating composition for antimicrobial containing an extract of Jangwi biloba or a fraction thereof as an active ingredient.

It is another object of the present invention to provide an antibacterial air cleaning article containing an extract of the monsoon nabe as an active ingredient.

In order to achieve the above object, the present invention January bibichu (Hosta capitata ) provides an antimicrobial and antifungal pharmaceutical composition containing the extract as an active ingredient.

In addition, the present invention provides an antimicrobial and antifungal pharmaceutical composition containing an organic solvent fraction prepared as an active ingredient by extracting the extracts of iljubeol as an organic solvent.

In addition, the present invention provides a topical antibacterial and antifungal skin preparation containing the extract of the monsoon nabe as an active ingredient.

In addition, the present invention provides a cosmetic composition for antibacterial and antifungal, which contains an extract of the monsoon nabe, or fractions thereof as an active ingredient.

In another aspect, the present invention provides a composition for oral antimicrobial containing iljubi nabe extract or a fraction thereof as an active ingredient.

In addition, the present invention provides a composition for antimicrobial antimicrobial filter coating containing an extract of the monsoon rain shine or fractions thereof as an active ingredient.

In addition, the present invention provides an antibacterial air-cleaning article containing an extract of the monsoon nabe as an active ingredient.

Sunshine of the present invention ( Hosta capitata ) extracts or fractions thereof inhibit the growth of bacteria and fungi that cause skin surface bacteria and fungi, food poisoning and floating fungi that inhabit filters, destroy the bacteria and fungal cells, and form mycelia of fungi. In addition, since the cell cycle was stopped at the G2 / M phase and induced apoptosis, it was confirmed that it has excellent antibacterial and antifungal activity. Can be used.

1 is a sun shining ( Hosta capitata ) is a graph showing the minimum inhibitory concentration of fungal activity of extracts or fractions thereof.
Figure 2 is a figure showing the effect of suppressing the allomorphic Candida albicans of Ilbebi beetle extract.
Figure 3 is a graph showing the effect of extracts from the monsoon rain on the fungal cell cycle.

Hereinafter, the present invention will be described in detail.

The present invention is January shinju ( Hosta capitata ) provides an antimicrobial and antifungal composition containing the extract as an active ingredient.

The fungus is Candida albicans ( C. albicans ), Saccharomyces cerevisiae ( S. cerevisiae ), E. coli , B. subtilis , Streptococcus mutans ( S. mutans ), S. aureus , Enterococcus hire , E. hirae , M. luteus and methicillin resistant staphylococcus aureus ), Propionibacterium acnes ), Epidermophyton floccosum ), Microsporum audouinii , Trichophyton ferrugineum ), Trichophyton mentagrophytes ), Trichophyton ruburm ), Staphylococcus aureus ), Staphylococcus epidermis , and Pityrosporum ovale), Candida albicans (candida albicans) and vaginal trichomonas (Trichomonas Vaginalis ) is preferably any one or more bacteria selected from the group consisting of, but is not limited thereto.

The sun beetle extract and its fractions are preferably prepared in the following steps, but is not limited thereto:

1) extracting the dried sun beetle by adding an extraction solvent;

2) filtering the extract of step 1); And

3) preparing the extract by concentrating the filtered extract of step 2) under reduced pressure; And

4) extracting the extract of step 3) with an additional organic solvent to prepare a fraction.

In the above-described method, the sun-shine can be used without limitation, such as cultivated or commercially available.

The extraction solvent is preferably water, alcohol or a mixture thereof, preferably a solvent selected from C ₁ to C ₄ lower alcohol or a mixed solvent thereof, and more preferably to extract with methanol or ethanol, but It is not limited. The amount of the extraction solvent is preferably 0.5 to 100 times the dry weight of the January shintobeol, and more preferably 5 to 10 times, but is not limited thereto. The extraction method may use a conventional extraction method such as supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction, preferably ultrasonic extraction method It can be extracted 1 to 5 times. When the extraction temperature is preferably 10 ℃ to 100 ℃ and more preferably room temperature, but is not limited thereto. The extraction time is preferably 2 hours to 24 hours, more preferably 3 hours, but is not limited thereto.

 In the above method, the reduced pressure concentration in step 3) is preferably a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably dried under reduced pressure, vacuum drying, boiling drying, spray drying, room temperature drying or lyophilization.

In the above method, the organic solvent of step 4) is preferably hexane (n-hexane), dichloromethane (dichloromethane) and ethyl acetate (ethyl acetate), more preferably ethyl acetate, but is not limited thereto. Do not.

In the above method, the fraction of step 4) is a 70% ethanol extract suspended in distilled water and mixed the same amount of hexane (n-hexane) and then put into a separatory funnel to separate and remove the hexane layer, the same amount in the remaining water layer After dichloromethane was mixed, the dichloromethane layer was separated, and then, the remaining amount of water was mixed with the same amount of ethyl acetate, and the ethyl acetate layer was separated, concentrated and freeze-dried. It is preferred to prepare fractions.

The fraction may be obtained by repeating the fractionation process from 1 to 5 times, preferably three times, from the sun beetle extract, and preferably concentrated under reduced pressure after fractionation, but not always limited thereto.

In a specific embodiment of the present invention, the sun shine was washed with water and then dried in the shade and at room temperature. The dried Sun Ljube was put in an extraction container, and extracted with an extraction solvent at 50 ~ 60 ℃ for 2 hours and filtered through a filter paper. The extract was concentrated under reduced pressure at 50-55 ° C. and lyophilized to prepare January Monchu extract.

In a specific embodiment of the present invention, the disk diffusion method was carried out to confirm the antimicrobial activity of the extract of the sun shinbeol. As a result, it was found that the extracts of Sunbi jade show high antibacterial activity, showing a large clear zone or almost no growth of bacteria.

In addition, MIC was determined to determine the minimum inhibitory concentration showing the antifungal activity of the extract of the monsoon beetle of the present invention and fractions thereof. As a result, it was confirmed that the monarch beetle extract and fractions thereof of the present invention has a high antimicrobial activity because it shows a low MIC value against harmful bacteria (see Fig. 1). In addition, the present inventors confirmed the antifungal activity by using the disk diffusion method because it is not easy to obtain MIC in the case of C. cladosporioides . As a result, it was confirmed that the sun shinbeol extract of the present invention and its fraction form a clear zone for Cladosporium cladosporioides, excellent antifungal activity.

In addition, it was confirmed that the effect on the allomorphic formation of Candida albicans of the extract of the January shinjubeol and its fractions of the present invention. As a result, it was confirmed that the January monbichu extract and fractions thereof of the present invention can prevent the Candida albicans bacteria to form allomorphs, thereby preventing the invention by Candida albicans in vivo (see FIG. 2).

In addition, in order to confirm the effect of the extract of the nasal nasal shinju of the present invention on the fungal cell cycle, after staining the DNA, the intensity of fluorescence was measured using flow cytometry. As a result, it was confirmed that the extract of the nasal nasal cavities of the present invention inhibits the division of fungal cells and causes cell death by stopping the cell cycle in G2 / M (see FIG. 3).

Therefore, January shines ( Hosta capitata ) extracts or fractions thereof inhibit the growth of bacteria and fungi that cause skin surface bacteria and fungi, food poisoning and floating fungi that inhabit filters, destroy the bacteria and fungal cells, and form mycelia of fungi. In addition, since the cell cycle was stopped at the G2 / M phase and induced apoptosis, it was confirmed that it has excellent antibacterial and antifungal activity. Can be used.

In addition, the present invention provides an antibacterial and antifungal skin external preparation containing the sun beetle extract or a fraction thereof as an active ingredient.

The fungus is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus and methicillin resistant Staphylo Caucus Arenas funny, propionic sludge tumefaciens arc Ness, epi more fur t flock koseom, My cross Forum Augusta also am Whoa, tree, second piton Ferrer Ginny Stadium, tree seconds piton menta him pie Massachusetts, a tree second piton rubeom, Staphylococcus aureus, It is preferably, but not limited to, any one or more bacteria selected from the group consisting of epidermal staphylococci, pytilosporum obale, Candida albicans and vaginal trichomonas.

Thus, the extracts of the monsoon rhizome, or fractions thereof, inhibit the growth of skin surface bacteria and fungi, the causative agents of food decay and food poisoning, and the floating fungi in the filter, and destroy the bacteria and fungal cells. Inhibiting mycelial formation, and also confirmed that it has excellent antibacterial and antifungal activity by stopping the cell cycle in the G2 / M phase and induces apoptosis. This can be useful for.

The external skin preparation is preferably an antibacterial and antifungal skin external preparation, characterized in that it is any one selected from the group consisting of solutions, suspensions, sprays, patches, pads, creams, ointments and gels. Do not.

In addition, the present invention provides a cosmetic composition for antimicrobial and antifungal, containing the monarch beetle extract or a fraction thereof as an active ingredient.

The cosmetic composition may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) A cream, a skin, a lotion, a powder, an ointment, a spray, or a cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition, in addition to the gardenia fraction of the present invention, in addition to fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic field.

In another aspect, the present invention provides a composition for oral antimicrobial containing iljubi nabe extract or a fraction thereof as an active ingredient.

The composition for oral cavity may be an oral composition for the purpose of preventing oral diseases or oral cleansing by removing bacteria and fungi in the oral cavity, characterized in that the one selected from the group consisting of toothpaste, mouthwash or mouthwash. It is preferable that the composition for oral use for an antimicrobial agent is not limited thereto.

In addition, the composition for oral cavity is an abrasive, wetting agent, foaming agent, binder, sweetening agent, pH adjusting agent, preservatives, medicinal ingredients, fragrances, brighteners, pigments, solvents, etc. commonly used in the field of oral nasal extracts or fractions thereof. May contain adjuvant.

In addition, the present invention provides a composition for antimicrobial antimicrobial filter coating containing an extract of the monsoon rain shine or fractions thereof as an active ingredient.

The antimicrobial filter coating composition may be a filter coating composition for the purpose of cleaning indoor air by removing floating bacteria and fungi, which is any one selected from the group consisting of solutions, suspensions, sprays and gels. It is preferred that the antimicrobial filter coating composition for antibacterial, characterized in that but not limited thereto.

In addition, the present invention provides an antibacterial air-cleaning article containing an extract of the monsoon nabe as an active ingredient.

The air cleaning product includes an air purifier or a filter for a central air conditioner, characterized in that the antibacterial and antifungal, characterized in that any one selected from the group consisting of a filter for a deodorant, deodorant, air purifier or air conditioner. Preferably, the antibacterial air cleaning article is not limited thereto.

Hereinafter, the present invention will be described in detail by the following Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples, Experimental Examples, and Preparation Examples.

< Example  1> Sun Moon shin extract or its Fraction  Produce

Purchasing Ilwolbechu cultivated at the Sancheong test site in Gangwon-do, and crushed to a suitable size, 10g of Sunwolbechu and 500 ml of 95% ethanol aqueous solution was added to the extraction vessel and reflux-cooled, filtered with a filter paper to obtain the extract. The extraction process was repeated three times, after which the solvent was concentrated under reduced pressure and dried to obtain 1 g of an aqueous ethanol extract.

The ethanol extract was subjected to a systematic fractionation and separated into fractions of n-hexane (34.6 mg), ethyl acetate (56.7 mg) and butanol (94.9 mg).

< Example  2> Preparation of strains for antimicrobial and antifungal experiments

Pathogenic strains of Candida albicans (Candida albicans ), Saccharomyces cerevisiae), Staphylococcus epidermidis (Staphylococcus epidermidis ) and the floating strain, Cladosporium cladosporioides , were distributed from Korea Genetic Resource Bank (KCTC) and used for the experiment.

< Experimental Example  1> Antibacterial and Antifungal Tests of Ilwolbechu Extracts by Disk Diffusion Method

In order to confirm the antimicrobial activity of the extracts and sun shinbeol of Example 1 prepared by the above method, the disk diffusion method was carried out as follows.

Overnight Candida Albi of Example 2 grown in liquid medium kanseu (C. albicans), saccharose in my process to the celebrity busy (S. cerevisiae), Staphylococcus epidermidis (S. epidermidis), Cloud FIG sports Solarium Cloud FIG Spokane Rioides ( Cladosporium cladosporioides ) were diluted to 1/10 each, and 100 μl of them were spread on agar medium. A round filter paper having a diameter of about 8 mm was soaked with 2 μl of a solution dissolved in DMSO so that the concentration of the extract or fraction prepared in Example 1 was 20 mg / ml, and then spread on agar medium. After culturing at 37 ° C. or 25 ° C. for 24 hours or 48 hours, the antimicrobial and antifungal activity was measured by measuring the part (clear zone) in which bacterial growth was inhibited.

The results are shown in Table 1. Antibiotic Mixture

Antibacterial and Antifungal Activity against the Different Fungi According to the Size of Clear Zone Strain name Antibiotic mixture ^{One )} Sun Moon Beetle Extract One S. epidermidis +++ ²⁾              + 2 S. aureus            +++              + 3 C. albicans            ++              ++ 4 S. cerevisiae            +++              +++ 5 C. cladosporioides            -              ++

The antibiotic mixture represented by 1) shows an impicillin (1 mg / ml), penicillin streptomycin (1 mg / ml) and amphotericin (250 mg / ml).

2) denotes the diameter of the suppressed zone as "-", 7 to 9 mm: "+", 9 to 10 mm: "++" and 10≤: "+++".

As shown in Table 1, in the case of Ilbeolbechu extract, it was confirmed that the antimicrobial activity is very good because it shows a wide transparent zone or almost no growth of bacteria.

< Experimental Example  2> minimum inhibitory concentration of the sample showing antifungal activity ( MIC , Minimum Inhibitory  Concentration Measurement

The minimum concentration (MIC) in which the bacteria did not grow was determined for the examples in which the antimicrobial and antifungal activity was confirmed by the disk diffusion methods of Experimental Examples 1 and 2.

The concentrations of the extracts or fractions thereof prepared in Example 1, starting at 200 μg / ml and continuing dilution to 2x concentrations, were lowered to 3.25 μg / ml and the Candida albicans and Saccharomyces cerevisiae to be used in the experiment. Incubated for 24 hours in advance to adjust the A ₆₀₀ to 0.1. The extract or fractions and strains thereof were incubated for 24 hours after the same amount of the mixture was mixed in a 96-well microplate. After 24 hours, the culture was diluted to 1/10 and dispensed into a new 96-well microplate, and the absorbance was measured at 600 nm to obtain MIC ₅₀ .

Absorbance measurements and MIC ₅₀ results are shown in FIGS. 1 and 2.

MIC ₅₀ measurement results (unit: μg / ml) Strain name Sample Name MIC ₅₀  (Μg / ml ) S. cerevisiae   Ethanol extract <3.13   Nucleic Acid Fraction 85.19   Ethyl acetate fraction > 200   Butanol fraction <3.13   Water fraction 157.36 C. albicans   Ethanol extract 24.18   Nucleic Acid Fraction > 200   Ethyl acetate fraction > 200   Butanol fraction 5.76   Water fraction > 200

As shown in Table 2, since the iljubeolcho extract or fractions thereof prepared in Example 1 has a low MIC value for all harmful bacteria used in the experiment, it was confirmed that the antibacterial activity is excellent. In addition, butanol fraction of the fractions showed the highest antimicrobial activity.

Therefore, it was confirmed that the sun shinbeol extract and its fractions can be usefully used as an antimicrobial composition.

< Experimental Example  3> Sunshine ratio using disk diffusion method Fraction  Antifungal activity

In the case of C. cladosporioides , it was not easy to obtain MIC on a liquid medium, and the disk diffusion method was used to confirm the antifungal activity of the fractions. Colonies of Cladosporium cladosporioides were diluted with physiological saline, and 100 μl of them were spread on agar medium. The concentration of the fractions prepared in Example 1 was adjusted to 20 mg / ml, soaked with a round filter paper having a diameter of about 8 mm with 2 μl, and then placed on agar medium to diffuse on agar medium. After 24 hours or 48 hours of incubation at 25 ° C, the area of the bacterial growth inhibited area (clear zone) was measured.

The results are shown in Table 3.

Antifungal Activity of the Monolithic Radix Fraction against Cladosporium Cladosporioides According to the Size of Clear Zone Sample Name Invisibility  size One   Ethanol extract ++ 2   Nucleic Acid Fraction - 3   Ethyl acetate fraction - 4   Butanol fraction ++ 5   Water fraction -

The inhibition zone diameter is shown as non-suppressive: "-", 7-9 mm: "+", 9-10 mm: "++", 10≤: "+++".

As shown in Table 3, since the sun beetle extract and butanol fraction prepared in Example 1 formed a clear zone for Cladosporium cladosporioides, it was confirmed that the antifungal activity is excellent.

Therefore, it was confirmed that the sun shinbeol extract and its fractions can be usefully used as an antimicrobial composition.

< Experimental Example  4> Sunbi aviation extract and its Fraction  effect

Candida albicans is necessary to form a dimorphic transition to induce pathogenic factors. Therefore, the following experiment was carried out to investigate the effect of the January Monbichu extract and its fractions on the allomorphic formation of Candida albicans. Candida albicans was incubated in YM medium, then diluted to a cell number of 3.5 × 10 ⁶ CFU / and the dilutions were dispensed into 24-well plates. 20% calf serum (Fetal bovine serum) was put in a similar environment in vivo, and the morphology of the fungus was observed by incubating in the incubator treated with 200 ㎍ / ml in 37 incubator at the same temperature.

As a result of performing the experiment, as shown in FIG. 2, when only the serum solution of the calf as a control was added, most Candida albicans bacteria were found to form alloforms and aggregated, whereas in the spheres treated with the extract of January shinju It was confirmed that allomorphic formation was inhibited. As a result, it was confirmed that the extract of the monarch beetle can prevent the onset by Candida albicans in vivo.

< Experimental Example  5> Influence of Ilwolbechu Extract on Fungal Cell Cycle

Candida albicans and Saccharomyces cerevisiae were incubated in YM medium so that the number of cells was 1 × 10 ⁹ cells / ml, and then treated with 10, 50, 100 μg / ml of the monthly rhizome extract for 24 hours at 24 ° C. After incubation, DNA was stained using propidium iodide, a DNA staining reagent, and the intensity of fluorescence was measured using flow cytometry.

As a result of the experiment, as shown in Figure 3, the cell cycle of Candida albicans and Saccharomyces cerevisiae G2 / M in the group treated with Jangwibeol extract, unlike the cell cycle of the control group not treated with any substance as shown in FIG. I could see that it stopped at the flag. In addition, the increase in the sub G1 phase, it was found that Jangwibichu extract induced epoptosis.

Therefore, it was confirmed that the extracts and fractions of the nasal nasal cavities of the present invention inhibit the division of fungal cells and cause cell death.

< Manufacturing example  1> Preparation of Pharmaceutical Formulations

<1-1> Powder  Produce

1 g of walnut beetle extract of the present invention

1 g lactose

The above components were mixed and packed in airtight bags to prepare powders.

<1-2> Preparation of Tablet

Sun Moon beet extract 100 mg of the present invention

Corn starch 100 mg

100 mg of milk

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 1-3 > Preparation of capsules

Sun Moon beet extract 100 mg of the present invention

Corn starch 100 mg

100 mg of milk

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

&Lt; 1-4 >

1 g of walnut beetle extract of the present invention

Lactose 1.5 g

1 g of glycerin

Xylitol 0.5 g

After mixing the above components, it was prepared to be 4 g per ring according to a conventional method.

<1-5> Preparation of granules

Ilbebibichu extract 150 mg of the present invention

Soybean Extract 50mg

Glucose 200 mg

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60 ° C. to form granules, which were then filled into fabrics.

< Manufacturing example  2> Cosmetics  Preparation of the composition

<2-1> Preparation of Cream

0.1 parts by weight of iljubeol extract, fractions thereof, or mixtures thereof of the present invention

Cetostearyl alcohol 2.8 parts by weight

Milnan 2.6 parts by weight

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

50.55 parts by weight of purified water

<2-2> Preparation of Lotion

0.1 parts by weight of iljubeol extract, fractions thereof, or mixtures thereof of the present invention

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

51.7 parts by weight of purified water

< Manufacturing example  3> Cosmetics  Preparation of Detergents in Compositions

<3-1> Preparation of soap

4.0 parts by weight of the extract of January shinjubeol, fractions thereof or mixtures thereof of the present invention

Soap base 94.25 parts

0.3 parts by weight of tocopherol acetate

Glycerin 0.2 parts by weight

0.8 parts by weight of PEG 4000 (polyethylene glycol 4000)

0.3 parts by weight of jojoba oil

Cetanol0.15 parts by weight

The components were mixed well in a mixer and then extruded in a soap making machine, cut and molded to a uniform size to prepare a soap composition. The soap base may be a sodium salt of palm oil, a natural fat or oil commonly used in the art.

<3-2> body Cleanser  Produce

4.0 parts by weight of the extract of January shinjubeol, fractions thereof or mixtures thereof of the present invention

Ammonium Lauryl Sulfate 42 parts by weight

Cocamidopropyl Betaine 12 parts by weight

0.5 parts by weight of glycol distearate

Propylene glycol 1.0 parts by weight

A proper amount of pigment

Fragrance

Proper amount of citric acid

1.0 part by weight of drain (50% concentrate)

100 parts by weight of purified water is added.

Ammonium lauryl sulfate, cocamidopropyl betaine, glycol distearate, and propylene glycol were heated to 70 ° C. and emulsified by adding heated purified water to emulsify and cooled to room temperature to prepare a body cleanser.

< Manufacturing example  4> Preparation of Oral Composition

<4-1> for oral cavity Gargle  Produce

Ilbebi beetle extract, fractions thereof, or mixtures thereof 0.1 to 1.0 g of the present invention

Xyitol 5-10 g

Ethyl alcohol 5 ~ 15 g

Sorbitol 5-15 g

Saccharin Sodium 10-100 mg

Sodium Monofluorophosphate 500 ~ 1000 mg

Sodium Lauryl Sulfate 100-200 mg

Polysorbate 20 100 ~ 1000 ㎎

Peppermint Flavor 10-100 mg

Sodium benzoate 10 ~ 100 g

A proper amount of pigment

Purified water is added to make 100 g.

According to the above-described method for preparing oral gargles according to the conventional oral composition was prepared gargles for oral compositions comprising the essential oil component of Dracosepalum poetidium, sun beetle extract or fractions thereof.

< Manufacturing example  5> For air cleaning  Manufacture of filter

5 parts by weight of acetic acid solution of the January nabechovy extract, fractions or mixtures thereof of the present invention was dissolved in 5 parts by weight: 95 parts each. The solution of the sun shin biloba extract, fractions thereof or a mixture thereof was extruded at a rate of 10 m / min into a platinum-gold alloy nozzle (0.1 mm φ × 300 holes) at room temperature. The extruded Sunil Biloba extract, fractions thereof, or mixtures thereof were solidified and solidified by neutralization in 5 wt% caustic soda.

Subsequently, the extruded Sunbi jade extract, its fractions or mixtures thereof are subjected to coagulation washing in a coagulation bath composed of water, and the flushed Sunbi jade extract, its fractions or mixture fibers thereof are maintained at a temperature of 98 ° C. Stretched in a boiling water bath consisting of water to obtain a wet fiber, 500 g of the actual weight of the wet fiber obtained a complex compound chelated saturated copper ions, the above-mentioned chelate-forming monobial extract, fractions thereof or their The fiber for the filter was prepared by cutting the mixture fiber.

Claims (17)

January bibichu (Hosta capitata ) Pharmaceutical composition for antibacterial and antifungal containing the extract as an active ingredient.
The method of claim 1, wherein the extract is the extract is water, C ₁ A pharmaceutical composition for antibacterial and antifungal, characterized in that extracted with a lower alcohol of ~ C ₄ or a mixed solvent thereof.
3. The antimicrobial and antifungal composition according to claim 2, wherein the lower alcohol is ethanol or methanol.
An antimicrobial and antifungal composition comprising an extract of an organic solvent, which is prepared by extracting the extract of the sun sol larvae as an organic solvent.
The antimicrobial and antifungal composition according to claim 4, wherein the organic solvent is hexane, ethyl acetate or butanol.
The method according to any one of claims 1 to 4, wherein the fungus is Candida albicans ( C. albicans ), S. cerevisiae ( S. cerevisiae ), E. coli , Bacillus subtilis (B. subtilis), Streptococcus mutans (S. mutans), Staphylococcus fringing-house (S. aureus), Enterococcus fin (E. hirae), the micro base Lactococcus Proteus (M. luteus) and methicillin Methicillin resistant staphylococcus (MRSA) aureus ), Propionibacterium acnes ), Epidermophyton floccosum ) Microsporum audouinii ), Trichophyton ferrugineum ), Trichophyton mentagrophytes ), Trichophyton ruburm ), Staphylococcus aureus ), Staphylococcus epidermis ), Pityrosporum ovale ), Candida albicans ( candida) albicans ) and vaginal trichomonas Vaginalis , antimicrobial and antifungal composition, characterized in that any one or more selected from the group consisting of.
An external antibacterial and antifungal skin preparation containing ilwolbibeop extract or a fraction thereof as an active ingredient.
8. The bacterium according to claim 7, wherein the bacterium is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus and Methicillin-Resistant Staphylococcus Areus, Propionibacterium Acnes, Epidermophyton Phlocosome, Microsforum Audonini, Trichophytonferargium, Trichophyton Mentagrophytes, Trichophyton Rubium Antibacterial and antifungal skin external preparation, characterized in that at least one bacteria selected from the group consisting of Staphylococcus aureus, Epidermal Staphylococcus, Pytilosporum obale, Candida albicans and Vaginal trichomonas.
An antibacterial and antifungal cosmetic composition containing an extract of the monsoon rain shine or fractions thereof as an active ingredient.
The bacterium according to claim 9, wherein the fungus is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus and Methicillin-Resistant Staphylococcus Areus, Propionibacterium Acnes, Epidermophyton Phlocosome, Microsforum Audonini, Trichophytonferargium, Trichophyton Mentagrophytes, Trichophyton Rubium Antibacterial and antifungal cosmetic composition, characterized in that any one or more bacteria selected from the group consisting of Staphylococcus aureus, Epidermal Staphylococcus, Pytilosporum obale, Candida albicans and Vaginal trichomonas.
An oral anti-oral composition containing an extract of January monsoon or a fraction thereof as an active ingredient.
The bacterium according to claim 11, wherein the fungus is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus And methicillin resistant Staphylococcus aerus, propionibacterium acnes, epidermophyton phlocosome, microsforum aurinini, trichophytonferraghenium, trichophyton mentagrophytes, trichophyton Antibacterial oral composition, characterized in that it is any one or more bacteria selected from the group consisting of Rubium, Staphylococcus aureus, Epidermal Staphylococcus, Pytilosporum Ovalle, Candida albicans and Vaginal Trichomonas.
Composition for coating antimicrobial antimicrobial filter containing ilwolbichu extract or a fraction thereof as an active ingredient.
14. The bacterium of claim 13, wherein the bacterium is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus and Methicillin-Resistant Staphylococcus Areus, Propionibacterium Acnes, Epidermophyton Phlocosome, Microsforum Audonini, Trichophytonferargium, Trichophyton Mentagrophytes, Trichophyton Rubium , Staphylococcus aureus, epidermal staphylococcus, phytirosporum obale, candida albicans and vaginal trichomonas is any one or more bacteria selected from the group consisting of antimicrobial antibacterial filter coating composition.
An antibacterial air cleaning article containing an extract of Ilwol beech as an active ingredient.
16. The antibacterial and air freshener of claim 15, wherein the air cleaning article is any one selected from the group consisting of filters for fragrances, deodorants, air cleaners, and ang air conditioners.
16. The bacterium according to claim 15, wherein the bacterium is Candida albican, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Enterococcus hyre, Micrococcus luteus And methicillin resistant Staphylococcus aerus, propionibacterium acnes, epidermophyton phlocosome, microsforum aurinini, trichophytonferraghenium, trichophyton mentagrophytes, trichophyton Antibacterial air cleaning article, characterized in that any one or more bacteria selected from the group consisting of Rubium, Staphylococcus aureus, Epidermal Staphylococcus, Pytilosporum Ovalle, Candida albicans and Vaginal Trichomonas.

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