KR20120007646A - The protein which uses the animal by-products which occurs from butchery process and amino acids extraction method - Google Patents

Abstract

PURPOSE: A method for preparing peptides using waste after slaughter is provided to enhance calcium content of vegetable by enhancing calcium absorption in crops and to save cost. CONSTITUTION: A method for preparing peptides using waste after slaughter comprises: a step of pulverizing the waste; a step of adding organic acid and protease to prepare hydrolysate; a step of mixing organic calcium to the hydrolysate to prepare a reactant; a step of performing ultrafiltration system; a step of maturing isolated reactant; and a step of maturing the reactant. The microorganism is Saccharomyces sake.

Description

The protein which uses the animal by-products which occurs from butchery process and amino acids extraction method}

The present invention relates to a peptide using a slaughter by-product waste and a method of manufacturing the same, and more particularly, to a peptide using a slaughter by-product to prepare a high-quality amino acid organic fertilizer and a functional feed from slaughter hairs such as piglet, feather and stepmother. And it relates to a manufacturing method thereof.

In general, in the agricultural field, acidification and environmental pollution of soils are caused by the use of chemical fertilizers and pesticides. It has been emerging.

In order to solve these problems, the need for alternative fertilizers is emerging, and in particular, the use of organic fertilizers, which are mainly composed of vegetable amino acids, as growth regulators of plants that are beneficial to crops and considering environmental pollution, is gradually increasing.

In recent years, organic fertilizers are fermented by mixing sawdust and food waste with servings of livestock and livestock. However, economical efficiency and efficiency have been reduced, and many technical improvements are required. In addition, low cost is required to increase the use of organic fertilizers. Development of various organic fertilizers is urgently needed.

Recently, various methods have been proposed for preparing organic fertilizers using animal proteins, that is, organic liquid fertilizers containing a large amount of amino acids that can be directly absorbed by plants by alkaline decomposition and enzymatic treatment of by-products obtained from animals at high temperatures. An example is Korean Patent Publication No. 1998-28705. However, most of these conventional methods have problems such as low processing efficiency, excessive cost in the manufacture of fertilizer, long time, and the external appearance of consumers due to severe odor caused by slaughter by-products.

On the other hand, calcium is a typical divalent cation metal, so that it is not easily absorbed by crops due to anion components or soils in the soil, and insoluble bonds with nitrogen, phosphoric acid, and trace elements frequently occur. It is also difficult to move, so it is difficult to absorb continuously.

If more than a certain amount of calcium is absorbed by vegetables during the growth of vegetables, the antagonism of not absorbing calcium works in the vegetables, and there has been a limitation in growing vegetables containing high calcium.

In addition, similar to the case of the calcium component, most crops tend not to absorb unnecessary components, so even if a fertilizer containing a large amount of a specific component has a disadvantage that the absorption into the crop does not occur well.

Therefore, the inventors of the present invention, while continuing to study organic fertilizers using animal proteins, constitute a circulatory system for the recycling of hydrolases that occupy a significant portion of the raw material costs in the hydrolysis and extraction step, to extract amino acids from slaughter by-product waste Continuous production has a cost reduction effect, using organic calcium as a calcium source for increasing the calcium content of vegetables, found a method for producing high quality functional amino acids, and completed the present invention.

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for producing peptides using slaughter by-products to produce high-quality amino acid organic fertilizers and functional feeds by allowing the production of high-quality amino acids from various slaughter by-products including pig hair, feathers, stepmother, and the like. It is to.

An object of the present invention is to provide a peptide bound to calcium using slaughter by-product waste prepared by the above method.

The present invention provides a method for producing peptides using slaughter by-products which enable the production of high-quality amino acid organic fertilizers and functional feeds from various slaughter by-products including pig hair, feather, stepmother, etc. To provide.

The method for producing a peptide of the present invention is characterized by using a hair including pig hair, feather, stepmother, etc. generated during the slaughter process.

Method for producing a peptide of the present invention comprises the steps of a) grinding slaughter by-product waste; b) preparing a hydrolyzate by adding an organic acid and a proteolytic enzyme to the ground product; c) mixing organic calcium with the prepared hydrolyzate to prepare a reactant; d) separating the reactant by filtering only a substance having a predetermined molecular weight or less using an ultrafiltration system; And e) aging the separated reactants in sequence.

The method for preparing a peptide of the present invention is characterized in that the first hydrolysis is carried out using boric acid as an organic acid during hydrolysis, and the second hydrolysis is performed using trypsin as a protease.

The method for producing a peptide of the present invention is characterized by preparing a peptide bound to calcium using organic calcium, wherein the organic calcium is characterized in that the oyster shell or starfish.

The method for producing a peptide of the present invention is characterized by filtration of only peptides having a molecular weight of 5.000 Da or less, and then aging the reactants by adding wood vinegar, atomized molasses, and microorganisms.

The method for producing a peptide of the present invention is characterized by using Saccharomyces shake ( Saccharomyces sake ) of the microorganisms during the aging of the reactants.

Hereinafter, the present invention will be described in detail.

a) a crushing process of pulverizing slaughter by-product waste;

b) a hydrolyzate manufacturing step of preparing a hydrolyzate by adding an organic acid and a proteolytic enzyme to the pulverized pulverized product;

c) a reaction step of preparing a reactant by mixing organic calcium with the prepared hydrolyzate;

d) a separation step of filtering and separating only those having a predetermined molecular weight or less using an ultra-filtering system to separate the reactants that have undergone the reaction process; And

e) a ripening step of ripening the separated reactant;

It provides a method for producing a peptide using the slaughter by-product waste produced, including.

The present invention provides a peptide coupled to calcium using the slaughtered by-product waste prepared by the method for producing a peptide. Please refer to Fig.

In the present invention, the slaughter by-product waste is a hair generated during the slaughter process, more specifically, characterized in that the use of the hair of the livestock, including pig hair, feather mother.

The hair of the livestock is physically and chemically stable because keratin is bound by peptide bonds that form β-structures as a main component and numerous hydrogen bonds and disulfide bonds in the chain. In order to efficiently decompose the hardly degradable keratin due to such structural stability, the present invention is prepared in the form of a peptide by primary hydrolysis through an organic acid, followed by second hydrolysis of the decomposition residue again using a proteolytic enzyme. It is characterized by producing value-added amino acids.

In the present invention, the organic acid in step b) is at least one selected from boric acid, lactic acid, uric acid and citric acid, preferably characterized in that boric acid, the proteolytic enzyme is trypsin or papain ( Papain), and preferably the proteolytic enzyme uses trypsin derived from an animal.

In the present invention, the step b) is stirred for 4 to 10 hours at 35 to 45 ℃ to prepare a hydrolyzate.

The boric acid of the present invention imparts a technical constitution that acts to gently adjust the pH during hydrolysis of pig hair, feather wool, etc., and to improve the quality of amino acids produced through chemical reaction with calcium in the hair.

In the present invention, the organic calcium of step c) is characterized in that 5 to 20 parts by weight with respect to 100 parts by weight of the hydrolyzate, characterized in that the organic calcium is selected from oyster shell or starfish.

In general, inorganic calcium sources such as hydrated lime are used as calcium sources of crops, but inorganic calcium sources are not only absorbed by absorption with other ingredients in the soil, but also inorganic calcium is absorbed by vegetables themselves. It is not good as a calcium source to increase the calcium content of vegetables because it does not work well.

The organic calcium used in the present invention is characterized by using waste fossil calcium and the like as an environmentally friendly organic calcium without using inorganic calcium as a source of calcium of vegetables, and its content is not only a synergistic effect due to functional mutual complement In consideration of the cost and effects of the reaction, it is characterized by using 5 to 20 parts by weight based on 100 parts by weight of the hydrolyzate.

In the present invention, the organic calcium is produced through the process of washing, calcining, grinding, etc. using an oyster shell, starfish, etc., is an alkaline organic calcium of pH 9 to 10 has a friendly nature with crops in the nature of the material In addition, the decomposition and absorption ability is far superior to mineral calcium, and has the advantage of actively growing the crop.

The organic calcium is preferably calcined at 800 to 1500 ℃ in order to remove foreign matters, water or toxic substances contained in the organic calcium. The organic calcium is ionized well enough to raise the pH to 13 to 14 after firing, thereby improving the absorption of calcium, and the content of calcium or trace elements per unit mass is increased due to water removal.

In the present invention, the reaction step of step c) is a hydrolyzate is decomposed into calcium, phosphorus and peptide, the decomposed peptide is phosphorylated by binding to phosphoric acid, the phosphorylated peptide is characterized in that the reaction with calcium It is done.

More specifically, calcium, phosphorus and peptides are extracted by the action of organic acids and proteolytic enzymes, and phosphorylated peptides are formed by the action of the extracted phosphorus and digested peptides. Is formed.

In the present invention, the separation step of step d) is characterized in that the molecular weight is less than 5.000 Da filtered, after the separation step, the unfiltered reactant is recycled to step b) is not filtered protease It is characterized in that the re-reaction.

More specifically, in the method for preparing a peptide of the present invention, after the decomposition and extraction of slaughter by-product waste, the entire process of separation and recovery of calcium-bound peptide consists of a single process system, and the reactants separated and recovered through the separation process. Silver is a calcium-binding peptide has the advantage that more than 90% is recovered from the fraction of 5000 Da or less.

In addition, the reaction product which is not finished in the separation process and cannot pass through the ultrafiltration membrane is re-introduced into the hydrolyzate manufacturing process step together with the protease (molecular weight 20000 to 30000 Da) and the reaction time until the end point of the desired decomposition. Will be extended. The hydrolase first introduced in the above process has the effect of reducing the production cost by participating in the reaction with the slaughtered by-product waste newly introduced into the hydrolyzate manufacturing process step through the recycling process.

In the present invention, the step e) is characterized in that by adding wood vinegar, atomized molasses, and microorganisms to the reactants to ripen.

The aging is added to 0.1 to 3% by weight of wood vinegar, 0.05 to 0.1% by weight of molasses, and 0.1 to 0.5% by weight of microorganisms, followed by low speed stirring for 2 to 4 hours to be aged at room temperature.

The wood vinegar is a liquid obtained by liquefying the smoke generated in the process of making charcoal by adding oak or conifers to the outside air, and the water content in commercial wood vinegar is generally 80 to 90%.

In the present invention, the wood vinegar was used as the moisture content of the wood vinegar 90%, the content is used 0.1 to 3% by weight. If the content of the wood vinegar is less than 0.1% by weight can not have the nutritional or immunological properties of the wood vinegar, more than 3% by weight does not further enhance the significant effect on the addition of wood vinegar and is not economical.

The molasses is used as an energy source of microorganisms during aging and used as an organic material (N, P, etc.), minerals (K, Ca, Mg, etc.), a component that plays a role of a vitamin supply agent, the content is 0.05 to 0.1% by weight Use If the content of molasses is less than 0.05% by weight, it is not sufficient to supply the molasses as an energy source for microorganisms or to supply nutrients such as organic matter, and when the content of the molasses exceeds 0.1% by weight, it does not further enhance the significant effect on the addition of molasses. .

In the present invention, the microorganism is Bacillus asidolactis ( Bacillus acidolactis), Bacillus subtilis sub NATO (Bacillus subtilis subsp natto ), Pseudomonas flurscens , Lactobacillus bulgaricus), Clostridium sub (Clostridium subsp ), Saccharomyces sake is characterized by using one or more microorganisms selected from the group.

More preferably the microorganism is no Saqqara MRS shake (Saccharomyces sake) in that, as the saccharide disclosed in my process Correa Taunus strain (Saccharomyces coreanus) Saito KFCC 11215, in my process serenity busy as Saccharomyces (Saccharomyces cerevisiae ) KFCC 11352 or Saccharomyces sake ) KFCC 11513 can be used.

In the present invention, the aging process using the Saccharomyces sake is to promote the physiological activity and growth of plants to increase the recovery of tissue cells weakened due to lack of sunshine, cold water, improve rooting and elongation and growth period By increasing the content of sulfur-containing amino acids such as cystine, methionine to increase, there is an effect that can increase the value of the fertilizer.

In the present invention, the aging product obtained after the aging process is a hydrated peptide having 30% solid content obtained by hydrolyzing keratin, a low-utility protein due to the crosslinked structure as a calcium-bound peptide. It is composed of up to 18 peptides, characterized by high quality functional peptides due to the effects of 18 kinds of balanced amino acid composition, various molecular lengths, organic calcium mixed in the hydrolysis process.

In the present invention, after the step e), it may further comprise a drying step of deodorizing and drying.

The drying process is performed using a spray drier, and the injection temperature is 200 ° C or higher, preferably 200 to 230 ° C, and the exhaust temperature is 150 ° C or higher, preferably 150 to 180 ° C at a relatively high temperature. In this way, excess organic acid is evaporated and removed, and the odor generated during the production of the calcium-bound peptide is halved, and a desirable direction is given to the calcium-bound peptide by the roasting effect under the spraying conditions. Is increased.

The method for producing a peptide according to the present invention has a cost reduction effect by continuously producing amino acids from slaughter by-product waste through a circulatory system for recycling a hydrolase which accounts for a substantial portion of raw material costs in the hydrolysis and extraction stages. Organic calcium can be used to prepare a functional peptide combined with calcium, which in itself has the advantage of increasing the calcium content of vegetables by increasing the calcium absorption rate in the crop.

The method for producing peptides according to the present invention prevents environmental pollution by efficiently producing high quality amino acids and proteins containing a large amount of ingredients beneficial to the human body from waste generated during slaughter of various livestock, High-quality amino acid organic fertilizers and functional feeds can be manufactured in a single process, resulting in economical efficiency.

1 is a schematic view of a process for preparing a peptide using slaughter by-product waste according to the present invention.

The present invention will be described in more detail with reference to the following examples. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

Hereinafter, the technical and scientific terms used herein will be understood by those skilled in the art without departing from the scope of the present invention. Descriptions of known functions and configurations that may be unnecessarily blurred are omitted.

[ Example ]

The hair generated during the slaughter of various livestock such as chickens, pigs, ducks, etc. was pulverized to a size of 1 to 2 mm with a grinder (water content of about 15%), and then the composition of Table 1 was compared with the weight of the pulverized product. % Organic acid and protein hydrolase were added, and 3 L of water per 1 kg of the total mixture was added and stirred at 40 ° C for 5 hours to hydrolyze.

10 parts by weight of organic calcium was mixed with respect to 100 parts by weight of the hydrolyzed hydrolyzate prepared in the composition of Table 1, and heated at 60 ° C. for 2 hours to prepare an ionized calcium aqueous solution.

The organic calcium was washed with oyster shells and starfish, fired at 1000 ° C., and ground to a size of 1 to 2 mm using a grinder to prepare alkaline organic calcium having a pH of 9 to 10.

The calcium aqueous solution prepared from the hydrolyzate of Preparation Examples 1 to 4 was passed through an ultrafiltration membrane (Membrane Filter: cutoff) having the sizes of 1,000, 3,000, 5,000, and 10,000 Da in Table 2 to separate and recover the peptide bound to calcium. In addition, the reaction which was not terminated and did not pass through the ultrafiltration membrane was reintroduced into the hydrolysis step together with the protease (molecular weight 20,000 to 30,000 Da).

After obtaining the reaction product recovered from Examples 1 to 16 of Table 2, 0.2% by weight of wood vinegar, 0.05% by weight molasses and Saccharomyces Correanus ( Saccharomyces) coreanus ) Saito KFCC 11215 0.1 wt% was added and aged at low speed for 5 hours to give a aging product.

[ Test Example  One]

With respect to the aging obtained from the reactants of Examples 1 to 16, the total production of the calcium-bound peptide contained in the obtained product was investigated.

As can be seen from the results in Table 3, the calcium-binding peptide was confirmed that the yield is increased in fractions of 5000 Da or less, in particular in the results of Examples 1 to 3, the boric acid of Preparation Example 1 At least 90% of the peptides bound to calcium could be recovered from the aged using the reactants.

The above results indicate that the production of slaughter by-product waste in the production method according to the present invention can effectively produce a large amount of calcium-binding peptide using boric acid and organic acid during hydrolysis as well as continuous production and cost reduction effects. In particular, the produced calcium-binding peptide is also confirmed that 90% or more can be obtained in fractions of 5000 Da or less.

[ Test Example  2]

In order to confirm the change in the composition of calcium according to the fertilization component for lettuce according to the use of the peptide of the present invention prepared in the above example was fertilized in the fertilization composition of Table 4 below. The basic fertilization was applied at 80 ㎏ per 100 pyeong during rotary work.The fertilizer was fertilized 5 times per 100 pyeong prior to 1 week before harvesting, and then harvested 1 week after the final fertilization to measure the calcium content of lettuce. Analyzed.

In the case of Example 17, the peptide liquid ratio of Example 1 used in Example 17 was used for the provision of the nitrogen component and the calcium component, and the conventional organic liquid ratio and the calcium preparation were used for the comparative example.

In the lettuce of Comparative Example 1 grown by a conventional cultivation method, the calcium content is 54 mg per 100 g, whereas the lettuce using the peptide liquid ratio of Example 1 according to the present invention has a calcium content of 195 mg per 100 g. It was confirmed that the very high.

The above results indicate that the peptide liquid fertilizer of Example 1 according to the present invention does not require the use of calcium preparations, and by itself increases the rate of calcium absorption in plants. It is also the result of confirming that it can be usefully used as an ingredient.

Claims (15)

a) a crushing process of pulverizing slaughter by-product waste;
b) a hydrolyzate manufacturing step of preparing a hydrolyzate by adding an organic acid and a proteolytic enzyme to the pulverized pulverized product;
c) a reaction step of preparing a reactant by mixing organic calcium with the prepared hydrolyzate;
d) a separation step of filtering and separating only those having a predetermined molecular weight or less using an ultra-filtering system to separate the reactants that have undergone the reaction process; And
e) a ripening step of ripening the separated reactant;
Method for producing a peptide using the slaughter by-product waste produced, including. The method of claim 1,
After the step e), the method of producing a peptide using slaughter by-product waste, characterized in that it further comprises a drying step of deodorizing and drying. The method of claim 1,
In step b), the organic acid is at least one selected from boric acid, lactic acid, uric acid, and citric acid, and the protease is trypsin or papain. Way. The method of claim 3, wherein
The organic acid is a method for producing a peptide using slaughter by-product waste, characterized in that boric acid. The method of claim 1,
The step b) is a method for producing a peptide using slaughter by-product waste, characterized in that the stirring for 4 to 10 hours at 35 to 45 ℃. The method of claim 1,
In the step c), the hydrolyzate is decomposed into calcium, phosphorus and peptides, and the decomposed peptide is phosphorylated by binding to phosphoric acid, and the phosphorylated peptide is slaughtered by-product waste, characterized in that a reaction occurs in combination with calcium. Method for producing a peptide using. The method of claim 1,
The organic calcium of step c) is a method for producing a peptide using slaughter by-product waste, characterized in that for mixing 5 to 20 parts by weight with respect to 100 parts by weight of hydrolyzate. The method of claim 7, wherein
The organic calcium of step c) is a method for producing a peptide using slaughter by-product waste, characterized in that selected from oyster shell or starfish. The method of claim 1,
The separation process of step d) is a method of producing a peptide using slaughter by-product waste, characterized in that only the molecular weight is filtered to less than 5.000 Da. The method of claim 9,
After the separation process, the unfiltered reactant is recycled to step b) and re-reacted with an unfiltered proteolytic enzyme. The method of claim 1,
The step e) is a method for producing a peptide using slaughter by-product waste, characterized in that by adding wood vinegar, finely divided molasses, and microorganisms to the reactants to ripen. 12. The method of claim 11,
The microorganism is Bacillus asidolactis ( Bacillus acidolactis), Bacillus subtilis sub NATO (Bacillus subtilis subsp natto ), Pseudomonas flurscens ), Lactobacillus bulgaricus), Clostridium sub (Clostridium subsp ), Saccharomyces sake ( Saccharomyces sake ) A method for producing a peptide using slaughter by-product waste, characterized in that using one or more microorganisms selected from the group. The method of claim 12,
The microorganism is a Saccharomyces sake ( Saccharomyces sake ) characterized in that the production method of peptides using slaughter by-product waste. The method according to any one of claims 1 to 13,
The slaughter by-product waste is a method for producing a peptide using slaughter by-product waste, characterized in that the hair generated during the slaughter process. A peptide bound to calcium using slaughter by-product waste prepared by the method according to any one of claims 1 to 13.

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