CN112352881A - Application of pepper processing by-product in biological feed - Google Patents

Application of pepper processing by-product in biological feed Download PDF

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Publication number
CN112352881A
CN112352881A CN202011358167.7A CN202011358167A CN112352881A CN 112352881 A CN112352881 A CN 112352881A CN 202011358167 A CN202011358167 A CN 202011358167A CN 112352881 A CN112352881 A CN 112352881A
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mixture
parts
enzyme
pepper processing
biological feed
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CN202011358167.7A
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张永清
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Luxian Yongqing Family Farm
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Luxian Yongqing Family Farm
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/149Jensenii
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Birds (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Sustainable Development (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to the technical field of utilization of food processing byproducts, and particularly discloses application of pepper processing byproducts in biological feed, wherein the pepper processing byproducts are used as an additive of the biological feed, and the additive comprises the following raw materials in parts by weight: 180 parts of pepper processing by-products, 1-5 parts of a first enzyme mixture, 3-8 parts of a probiotic mixture and 10-15 parts of a second enzyme mixture; the preparation method of the additive is also disclosed, wherein pepper processing byproducts are used as raw materials, and a carrier is obtained by treating a part of pepper processing byproducts through an enzyme mixture and a probiotic mixture, so that the prepared carrier has appropriate grease and crude fiber content, good looseness and adsorbability, strong palatability after being added into feed, and improved animal growth performance. The byproduct of pepper processing, which is used as the raw material, is comprehensively utilized, and nutrients and active ingredients in the byproduct are fully utilized, so that the aim of environmental protection is fulfilled, and the effective utilization of resources is realized.

Description

Application of pepper processing by-product in biological feed
Technical Field
The invention relates to the technical field of utilization of food processing byproducts, in particular to application of a pepper processing byproduct in biological feed.
Background
The pepper is a rutaceae plant and contains various active ingredients, the fresh pepper is not easy to store and is easy to brown and mildew at room temperature, and the fresh pepper needs to be stored at low temperature in time after being picked, so that the fresh fruit is usually treated by directly preparing the fresh fruit into pepper oil or naturally airing the fresh fruit to prepare dry pepper, and a large amount of volatile ingredients of the pepper can be lost in the natural airing process. With the continuous increase of the yield of the zanthoxylum, the method for finely and deeply processing the zanthoxylum is continuously promoted to be new, and simultaneously, a large amount of zanthoxylum processing byproducts are generated.
The pepper processing by-products contain higher content of protein, grease, polysaccharide, crude fiber and other active ingredients (such as flavone, polyphenol, alkaloid and the like), and the by-products have great recycling value without the action of high temperature and chemical reagents in the cold pressing process of pepper oil. In the prior art, pepper meal is used for preparing animal feed, namely pepper meal is used as a main raw material and is prepared by mixing with other auxiliary materials after fermentation, the methods mainly utilize protein in pepper processing byproducts, but the content of crude fiber in the byproducts is far higher than that of the protein, and the crude fiber component in the pepper processing byproducts can not be well utilized because the addition amount of the pepper processing byproducts is higher in the existing application method and the crude fiber is decomposed for improving the palatability of the feed. Therefore, in order to further improve the utilization of the pepper processing by-products, a utilization method capable of fully utilizing the proteins in the by-products is urgently needed to be found.
Disclosure of Invention
In order to solve the problems in the background art, the invention aims to provide an application of a pepper processing byproduct in biological feed.
In order to achieve the purpose, the invention adopts the technical scheme that:
an application of the by-product of the pepper processing in biological feed, wherein the by-product of the pepper processing is used as an additive of the biological feed.
Further, the additive of the biological feed comprises the following raw materials in parts by weight: 180 parts of pepper processing by-products, 1-5 parts of a first enzyme mixture, 3-8 parts of a probiotic mixture and 10-15 parts of a second enzyme mixture.
Furthermore, the pepper processing by-product is powder which is crushed after being sterilized and dried.
Further, the first enzyme mixture comprises Aspergillus niger lipase and Penicillium cyclopium lipase, the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 3:1-1:1, and the enzyme addition amount is 6000-1000U/g.
Further, the probiotic mixture comprises saccharomyces boulardii, lactobacillus jensenii.
Furthermore, the second enzyme mixture is a protease mixture comprising plant proteolytic enzyme and serine protease, and the adding ratio of the plant proteolytic enzyme to the serine protease is 1:1-1:2, and the enzyme adding amount is 12000-15000U/g.
Further, the preparation method of the biological feed additive comprises the following steps:
drying the pepper processing by-products by using mould, and crushing into powder;
uniformly mixing the first weight part of the crushed pepper processing by-product with a first enzyme mixture, a probiotic mixture and distilled water, and reacting to obtain a first intermediate;
filtering the first intermediate, drying and sterilizing the solid matter, and pulverizing into 80-90 mesh powder to obtain a first mixture;
preparing the rest of the crushed pepper processing byproducts in parts by weight into suspension, adding a second enzyme mixture for hydrolysis, and drying to obtain a second mixture; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
Further, the first weight part of the crushed pepper processing by-products is 85-100 parts.
Further, the material-liquid ratio of the first weight part of the crushed pepper processing by-product to distilled water is 1:1, the reaction temperature of the obtained first intermediate is 28 ℃, and the reaction time is 8-10 h.
Further, the temperature for adding the second enzyme mixture for hydrolysis is 40-55 ℃, the reaction time is 6-8h, and the pH is 8.0-8.5.
The biological feed additive prepared by using the pepper processing byproducts is added into the biological feed, each 1000kg of the biological feed contains 8-30kg of the additive, and different addition amounts are adjusted to meet the feeding requirements of chickens, pigs, cattle, sheep and the like.
Compared with the prior art, the invention has the beneficial effects that:
the method takes pepper processing byproducts as raw materials, the pepper processing byproducts contain high-content crude fibers and proteins, a part of the pepper processing byproducts is treated by adding aspergillus niger lipase, penicillium cyclopium lipase and probiotic mixture, so that the degradation of grease and amide substances in the pepper processing byproducts is realized, and the pepper processing byproducts can be used as carriers of biological feeds after being crushed; through mixing of the enzyme mixture and the probiotic mixture and strict control of the addition amount, the prepared carrier oil and crude fiber have appropriate content, good loose property and adsorbability, strong palatability after being added into feed, and improved animal growth performance. The residual pepper processing by-products are hydrolyzed by adding the compound protease, the pepper active peptide is obtained by strictly controlling the variety, the dosage and the reaction environment of each protease, and the palatability of the feed can be further improved and the organism growth can be promoted after the pepper active peptide is added into the biological feed. Finally, the byproduct of pepper processing, which is used as the raw material, is comprehensively utilized, and the nutrient substances and active ingredients in the byproduct are fully utilized, so that the aim of environmental protection is fulfilled, and the effective utilization of resources is realized.
Detailed Description
For further understanding of the present invention, the method and effects of the present invention will be described in further detail with reference to specific examples. It should be noted that the present embodiment is only for further illustration of the present invention and should not be construed as limiting the scope of the present invention, and that those skilled in the art can make modifications and adjustments in a non-essential way based on the above disclosure.
Example 1
An application of byproduct of fructus Zanthoxyli in biological feed, wherein the byproduct of fructus Zanthoxyli is used as additive of biological feed; the additive of the biological feed comprises the following raw materials in parts by weight: 100-150 parts of pepper processing by-products, 1-5 parts of a first enzyme mixture, 3-8 parts of a probiotic mixture and 10-15 parts of a second enzyme mixture.
Wherein the pepper processing by-product is pulverized powder after being sterilized and dried; the first enzyme mixture comprises Aspergillus niger lipase and Penicillium cyclopium lipase, the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 3:1-1:1, and the enzyme addition amount is 6000-1000U/g; the probiotic mixture comprises saccharomyces boulardii, lactobacillus jensenii; the second enzyme mixture is a protease mixture and comprises plant proteolytic enzyme and serine protease, the addition ratio of the plant proteolytic enzyme to the serine protease is 1:1-1:2, and the enzyme addition amount is 12000-15000U/g.
As used herein, Aspergillus niger lipase was purchased from Hangzhou Chuangke Biotechnology, Inc., Penicillium cyclopium lipase was purchased from Beijing Pan-sphere Biotechnology, Inc., phytoproteolytic enzyme and serine protease were purchased from Cangzhou Xiaheng enzyme Biotechnology, Inc., and Saccharomyces boulardii and Lactobacillus jensenii were purchased from Tian Jianyi (Xiamen) Biotechnology, Inc.
Example 2
The preparation method of the biological feed additive comprises the following steps:
drying the pepper processing by-products by using mould, and crushing into powder;
uniformly mixing the first weight part of the crushed pepper processing by-product with a first enzyme mixture, a probiotic mixture and distilled water, and reacting to obtain a first intermediate;
filtering the first intermediate, drying and sterilizing the solid matter, and pulverizing into 80-90 mesh powder to obtain a first mixture;
preparing the rest of the crushed pepper processing byproducts in parts by weight into suspension, adding a second enzyme mixture for hydrolysis, and drying to obtain a second mixture; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
Wherein the first weight part of the crushed pepper processing by-products is 85-100 weight parts; the feed-liquid ratio of the first weight part of the crushed pepper processing by-product to distilled water is 1:1, the reaction temperature of the first intermediate is 28 ℃, and the reaction time is 8-10 h; the temperature for adding the second enzyme mixture for hydrolysis is 40-55 ℃, the reaction time is 6-8h, and the pH is 8.0-8.5.
Example 3
The preparation method of the biological feed additive comprises the following steps:
drying the pepper processing by-products by using mould, and crushing into powder;
taking 85g of crushed pepper processing byproducts, uniformly mixing the crushed pepper processing byproducts with a mixture of aspergillus niger lipase and penicillium cyclopium lipase, a mixture of 3g of probiotics and distilled water according to the material-liquid ratio of 1:1, and reacting at 28 ℃ for 8 hours to obtain a first intermediate; wherein the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 1:1, and the enzyme addition amount is 7000U/g; the probiotic mixture is saccharomyces boulardii and lactobacillus jensenii, and the ratio is 1: 1;
filtering the first intermediate, keeping the solid matter, drying, sterilizing, and pulverizing into 80 mesh powder to obtain a first mixture;
preparing 20g of crushed pepper processing byproducts into suspension, adding plant protein hydrolase and serine protease, reacting at the temperature of 40 ℃ for 8h at the pH value of 8.0, and drying to obtain a second mixture; wherein the adding ratio of the plant proteolytic enzyme to the serine protease is 1:1, and the enzyme adding amount is 12000U/g; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
Example 4
The preparation method of the biological feed additive comprises the following steps:
drying the pepper processing by-products by using mould, and crushing into powder;
uniformly mixing 100g of crushed pepper processing byproducts with a mixture of aspergillus niger lipase and penicillium cyclopium lipase, 8g of a probiotic mixture and distilled water according to a material-liquid ratio of 1:1, and reacting at 28 ℃ for 10 hours to obtain a first intermediate; wherein the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 3:1, and the enzyme addition amount is 1000U/g; the probiotic mixture is saccharomyces boulardii and lactobacillus jensenii, and the ratio is 1: 1;
filtering the first intermediate, keeping the solid matter, drying, sterilizing, and pulverizing into 90 mesh powder to obtain a first mixture;
preparing 50g of crushed pepper processing byproducts into suspension, adding plant protein hydrolase and serine protease, reacting at 50 ℃ and pH8.5 for 6h, and drying to obtain a second mixture; wherein the ratio of the plant proteolytic enzyme to the serine protease is 1:1, and the enzyme addition amount is 15000U/g; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
Example 5
The preparation method of the biological feed additive comprises the following steps:
drying the pepper processing by-products by using mould, and crushing into powder;
uniformly mixing 90g of crushed pepper processing byproducts, a mixture of aspergillus niger lipase and penicillium cyclopium lipase, 5g of a probiotic mixture and distilled water according to a material-liquid ratio of 1:1, and reacting at 28 ℃ for 9 hours to obtain a first intermediate; wherein the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 2:1, and the enzyme addition amount is 6500U/g; the probiotic mixture is saccharomyces boulardii and lactobacillus jensenii, and the ratio is 1: 1;
filtering the first intermediate, keeping the solid matter, drying, sterilizing, and pulverizing into 85 mesh powder to obtain a first mixture;
preparing 30g of crushed pepper processing byproducts into suspension, adding plant protein hydrolase and serine protease, reacting at 45 ℃ and pH8.2 for 7h, and drying to obtain a second mixture; wherein the ratio of the plant proteolytic enzyme to the serine protease is 1:1, and the enzyme addition amount is 15000U/g; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
Comparative example 1
Compared with the example 3, the probiotic mixture is not added, and the rest of the formula and the process are the same as the example 3.
Test examples
The biological feed additives prepared in examples 3 to 5 and comparative example 1 were added to a common biological feed in a ratio of 15kg of the biological feed additive per 1000kg of the biological feed, respectively. 320 healthy 65-day-old nursery pigs with the weight of 25kg were selected, randomly divided into 4 groups of 4 replicates each, and fed with the feed described above for 20 replicates each, the test time was 100 days, the initial weight, the end weight and the feed consumption of each group of test pigs were measured and recorded, the Average Daily Gain (ADG), the Average Daily Feed Intake (ADFI) and the consumption-to-weight ratio (F/G) were calculated, and the test results are shown in table 1.
TABLE 1
Figure DEST_PATH_IMAGE002
As can be seen from the results in Table 1, the biological feed additive prepared by the example of the present invention, when added to a biological feed, enables the ending body weight of the test pigs of examples 3-5 to be significantly higher than that of comparative example 1, and the ADFI has no significant difference, but the value of F/G is also significantly lower than that of comparative example 1, that is, the biological feed additive prepared by the present invention, when added to the feed, can significantly improve the growth performance of nursery pigs.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. The application of the pepper processing by-product in the biological feed is characterized in that the pepper processing by-product is used as an additive of the biological feed.
2. The use according to claim 1, wherein the supplement to the biological feed comprises the following raw materials in parts by weight: 180 parts of pepper processing by-products, 1-5 parts of a first enzyme mixture, 3-8 parts of a probiotic mixture and 10-15 parts of a second enzyme mixture.
3. The use according to claim 2, wherein the byproduct of zanthoxylum bungeanum processing is pulverized powder after sterilization and drying.
4. The use according to claim 2, wherein the first enzyme mixture comprises Aspergillus niger lipase and Penicillium cyclopium lipase, the ratio of the Aspergillus niger lipase to the Penicillium cyclopium lipase is 3:1-1:1, and the amount of the enzyme is 6000-1000U/g.
5. The use as claimed in claim 2, wherein said probiotic mixture comprises Saccharomyces boulardii, Lactobacillus jensenii.
6. The use according to claim 2, wherein the second enzyme mixture is a protease mixture comprising a plant proteolytic enzyme and a serine protease, and the ratio of the plant proteolytic enzyme to the serine protease is 1:1-1:2, and the enzyme amount is 12000-15000U/g.
7. The use according to any of claims 1 to 6, wherein the biological feed supplement is prepared by a process comprising the steps of:
drying the pepper processing by-products by using mould, and crushing into powder;
uniformly mixing the first weight part of the crushed pepper processing by-product with a first enzyme mixture, a probiotic mixture and distilled water, and reacting to obtain a first intermediate;
filtering the first intermediate, drying and sterilizing the solid matter, and pulverizing into 80-90 mesh powder to obtain a first mixture;
preparing the rest of the crushed pepper processing byproducts in parts by weight into suspension, adding a second enzyme mixture for hydrolysis, and drying to obtain a second mixture; and
and uniformly mixing the first mixture and the second mixture to obtain the biological feed additive.
8. The use according to claim 7, wherein the first part by weight of the pulverized byproduct of Zanthoxylum bungeanum is 85-100 parts by weight.
9. The use according to claim 7, wherein the feed-to-liquid ratio of the first part by weight of the pulverized byproduct of Zanthoxylum bungeanum to distilled water is 1:1, the reaction temperature of the first intermediate is 28 ℃, and the reaction time is 8-10 h.
10. The use according to claim 7, wherein the hydrolysis with the addition of the second enzyme mixture is carried out at a temperature of 40-55 ℃, a reaction time of 6-8h and a pH of 8.0-8.5.
CN202011358167.7A 2020-11-27 2020-11-27 Application of pepper processing by-product in biological feed Pending CN112352881A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170119020A1 (en) * 2014-05-30 2017-05-04 Cargill, Incorporated Method of feeding an animal
CN109221615A (en) * 2018-09-30 2019-01-18 南宁学院 A kind of Chinese prickly ash dregs of rice pannage
CN109953187A (en) * 2017-12-26 2019-07-02 湖南泰华科技检测有限公司 A kind of polypeptide feed addictive and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170119020A1 (en) * 2014-05-30 2017-05-04 Cargill, Incorporated Method of feeding an animal
CN109953187A (en) * 2017-12-26 2019-07-02 湖南泰华科技检测有限公司 A kind of polypeptide feed addictive and preparation method thereof
CN109221615A (en) * 2018-09-30 2019-01-18 南宁学院 A kind of Chinese prickly ash dregs of rice pannage

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