KR20110115722A - Cosmetic composition for reducing skin wrinkle comprising beta-lapachone - Google Patents

Abstract

The present invention relates to a skin external preparation composition and cosmetic composition for promoting collagen biosynthesis using beta-rapacone as an active ingredient, and the composition promotes collagen synthesis, thereby further relieving skin wrinkles and maintaining firm and elasticity. Do it

Description

Cosmetic composition for anti-wrinkle comprising beta- rapacone as an active ingredient {Cosmetic Composition for Reducing Skin Wrinkle Comprising beta-Lapachone}

The present invention The present invention relates to a skin external preparation composition and a cosmetic composition for promoting collagen production, including beta-rapacone.

Skin is the largest tissue in the envelope system consisting of a number of epithelial tissues that protect the muscles and organs in the body, and plays an important role in maintaining homeostasis by protecting the body from the external environment. As a result, the skin is degraded due to external physical and chemical stimuli, stress, and nutritional deficiency, and skin aging such as elasticity reduction and wrinkle formation is promoted.

Aging of the skin can be divided into innate age and extrinsic age. The endogenous aging is aging that occurs as the age increases, and aging occurs due to a decrease in the physiological function of the body. In addition, the exogenous aging refers to an aging phenomenon generated by external ultraviolet light, free radicals, stress, and the like. Aging of the skin proceeds by these endogenous and exogenous aging factors, and as a result, wrinkles are formed on the surface of the skin, resulting in rough skin and reduced elasticity.

According to the mechanism by which wrinkles are formed, collagen and elastin in the dermis continue to be degraded and produced. If the supplementation caused by the production after decomposition is not smooth, the skin loses its elasticity and wrinkles Is generated.

Representative anti-wrinkle and skin anti-aging agents studied so far include retinoic acid, retinol, ascorbic acid, α-hydroxy acid, and the like.

Retinoic acid inhibits lipid per-oxidation and ornithine decarboxylase response. However, the side effect of reddening the skin due to the sensitive reaction and high irritation by ultraviolet light has been reported, the use is limited, and there is a problem that the effect appears only after 6 months of continuous use.

Retinol has a weaker wrinkle improvement effect than retinoic acid, but is widely used because of its relatively high safety. However, when retinol is used in high concentrations, skin irritation occurs, skin pigmentation occurs when exposed to ultraviolet rays, and there is a problem in stability because it is easily oxidized by oxygen, heat and light in the air.

Ascorbic acid is easily oxidized by the external environment, such as air, moisture, high temperature, and thus the activity is greatly reduced, so that the degree of wrinkle improvement is lowered, and there is a problem of causing discoloration and discoloration of the formulation.

In addition, α-hydroxy acid is also disclosed as an anti-wrinkle agent, but there are problems in terms of safety of the product, accompanied by side effects of skin irritation such as itching, erythema, and sensitive reaction to ultraviolet rays.

In addition, cosmetics containing sunscreens, moisturizers, antioxidants, etc. are widely used to prevent wrinkles, but the actual wrinkle improvement of these products is insignificant.

The present inventors conducted a study for applying to the cosmetic composition, including the external preparation for the skin by confirming the effect of beta-rapacone to promote collagen production while screening various compounds.

Beta-lapachol is a lapacho tree, Tabebuia Natural quinone substance obtained from avellandedae ,), which is extracted from various plants.

Anti-cancer effects of beta-rapacone [Terai, Kaoru et al. al ., Cisplatin enhances the anticancer effect of beta - lapachone by upregulating NQO1 . , Anticancer Drugs , Nov, 01; 20 (10): 901-9 (2009); United States Patent Nos. 7,074,824, 6,962,944, 6,664,288, 7,361,691, WO 2003/11224, Antitumor effect [HR Shah et. al ., Beta - lapachone inhibits proliferation and induces apoptosis in retinoblastoma cell linesBeta - lapachone in retinoblastoma cell lines , Eye 22, 454-460 (March 2008); United States Patent Publication Nos. 2009/0226395, 7,070,797], anti-inflammatory effects [Dong-Oh Moon et al ., Anti-inflammatory effects of β- lapachone in lipopolysaccharide - stimulated BV2 microglia , International Immunopharmacology , Vol 7, Issue 4, 506-514, (April 2007)], antifungal effect [Shing-Hwa Liu et al ., Inhibition of inducible nitric oxide synthase by β-lapachone in rat alveolar macrophages and aorta , Br J Pharmacol . 126 (3): 746-50. (February 1999).

However, even in the above studies, there was no study on the skin wrinkle improvement effect of beta- rapacon, the active ingredient of the present invention.

US Patent No. 7,074,824; US Patent No. 6,962,944; US Patent No. 6,664,288; US Patent No. 7,361,691; International Patent Publication WO 2003/11224; United States Patent Publication No. 2009/0226395 United States Patent No. 7,070,797

Terai, Kaoru et al., Cisplatin enhances the anticancer effect of beta-lapachone by upregulating NQO1., Anticancer Drugs, Nov, 01; 20 (10): 901-9 (2009); H R Shah et al., Beta-lapachone inhibits proliferation and induces apoptosis in retinoblastoma cell lines, Beta-lapachone in retinoblastoma cell lines, Eye 22, 454-460 (March 2008); Dong-Oh Moon et al., Anti-inflammatory effects of β-lapachone in lipopolysaccharide-stimulated BV2 microglia, International Immunopharmacology, Vol 7, Issue 4, 506-514, (April 2007); Shing-Hwa Liu et al., Inhibition of inducible nitric oxide synthase by β-lapachone in rat alveolar macrophages and aorta, Br J Pharmacol. 126 (3): 746-50. (February 1999)

The present inventors have completed the present invention by confirming that beta-rapacone promotes collagen production and shows an improvement in skin wrinkles when applied to the skin.

Therefore, an object of the present invention is to provide an external composition for promoting collagen synthesis containing beta-rapacone as an active ingredient.

In addition, another object of the present invention to provide a cosmetic composition for improving wrinkles comprising beta- rapacone as an active ingredient.

In order to achieve the above object, the present invention provides a skin external composition for promoting collagen synthesis comprising beta- rapacone represented by Formula 1 as an active ingredient:

In addition, the present invention provides a cosmetic composition for improving wrinkles comprising beta- rapacone represented by Formula 1 as an active ingredient.

The external preparation composition and the cosmetic composition according to the present invention act on the fibroblasts of the dermal layer to promote collagen production, and show an excellent effect of promoting collagen production at low concentrations compared to the conventional anti-wrinkle retinol, and thus the wrinkle improvement effect is exceptional. In addition, the formulation stability is excellent and there are few side effects on the skin.

Beta-rapacone proposed in the present invention is 3,4-dihydro-2,2-dimethyl-2H-naphtho (1,2-b) pyran-5,6-dione (3, 4-Dihydro-2,2-dimethyl-2H-naphtho (1,2-b) pyran-5,6-dione) is a compound:

[Formula 1]

The beta-Rapa cones can be purchased that is a CAS No. 1707-32- 8 of known materials that are commercially available or extracted from natural plants.

Beta extracted from natural plant-Rapa cone bignoniaceae (Bignoniaceae), verbenaceae (Verbenaceae), proteaceae (Proteaceae), leguminous (Lequminosae), sandpaper tagwa (Sapotaceae), Scrophulariaceae (Scrophulariaceae), Malvaceae (Malvaceae), etc. Can be obtained by extracting from plants.

For example, using conventional methods known in the art, that is, using a conventional solvent, it can be prepared by methods such as hot water extraction, room temperature extraction, warm extraction, ultrasonic extraction, supercritical extraction, etc. which are commonly used.

Preferably it is extracted using a solvent selected from the group consisting of water, lower glycols having 1 to 6 carbon atoms, lower alcohols having 1 to 4 carbon atoms, and mixtures thereof. Most preferably, 1,3-butylene glycol is used as the extraction solvent, and extracted with an ultrasonic extractor at room temperature for 1 to 12 hours. The time of the ultrasonic extraction may vary depending on the amount of sample to be extracted, and may undergo a process of filtering or purifying the extraction result.

In addition, commercially available beta- rapacon is used after purification after purchase. The purification method used at this time may be a method commonly used in this field. Liquid-liquid extraction using a mixture of various organic solvents, purification methods such as column chromatography and high-performance liquid chromatography (HPLC) filled with various synthetic resins, such as silica gel and activated alumina, are not limited to the above purification methods. .

Experimental Example 1 in accordance with the present invention to test the collagen synthesis effect of beta- rapacon, it is proved that the collagen biosynthetic effect of beta- rapacon is superior to the retinol used as a conventional material for promoting collagen production It was.

Through these results, the beta-rapacone proposed in the present invention can be applied as an external skin composition and a cosmetic composition, and when applied to the skin, it promotes collagen synthesis, thereby further relieving skin wrinkles, making it firm and elastic. You can get the effect of keeping it.

Beta-Rapacon may comprise 0.00001 to 10.0% by weight, preferably 0.001 to 0.1% by weight relative to the total weight of the external preparation composition. If the content of the compound is less than the above range can not expect a distinct effect, on the contrary, if it exceeds the above range, the increase of the effect is less than the increase of the amount of inclusions and may cause some cytotoxicity.

The external preparation composition is not particularly limited in its formulation, and may be, for example, an external preparation composition having a formulation of body lotion, ointment, gel, cream, patch or spray. At this time, in the external preparation composition of each formulation, other components than the above-mentioned beta- rapacon may be appropriately selected by those skilled in the art according to the formulation or purpose of use of other external preparations.

In particular, beta-rapacone according to the present invention can be applied as a cosmetic composition comprising it as an active ingredient through the collagen synthesis promoting effect.

As a result of applying the cream containing beta- rapacon to the skin in Experimental Example 2 according to the present invention showed a high wrinkle improvement effect, and measured the skin irritation in Experimental Example 3 confirmed that the compound does not cause skin irritation It was.

Beta-Rapacon may comprise 0.00001 to 10.0% by weight, preferably 0.001 to 0.1% by weight relative to the total cosmetic composition weight. If the content of beta-rapacone is less than the above range, no obvious effect can be expected. On the contrary, if the content of the beta-rapacone exceeds the above range, the increase in the effect is less than that of the increase in the amount of inclusion, and the cytotoxicity is slightly reduced.

The cosmetic composition is not particularly limited in the formulation thereof, for example, lotion, milky lotion, cream, foam, essence, serum, softening water, milky lotion, foundation, makeup base, soap, sunscreen cream and sun oil It may be prepared in a dosage form.

In addition, the compositions of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, and fungicides within the scope of not impairing the effect thereof. , Oxidative stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, antifoams, humectants, vitamins And known compounds such as insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basicizing or acidifying agents, or colorants.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to beta-rapacone as an active ingredient, and include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The compositions of the present invention may be prepared in any formulations commonly prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The beta-rapacone of the present invention was very excellent in promoting collagen and elastin biosynthesis (see Experimental Example 1), effectively improved skin wrinkles and promoted elasticity (see Experimental Example 2), almost irritation to human skin No (see Experimental Example 3), it can be seen that the stability in the formulation is excellent (see Experimental Example 4).

[Example]

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Experimental Example  One: Type  I collagen  Biosynthesis promoting effect

In order to determine the collagen synthesis effect of beta-rapacone according to the present invention, it was purchased from Sigma Aldrich and purified.

Human normal fibroblasts were inoculated into 48-well microplates containing DMEM medium containing 10% fetal bovine serum (1 × 10 ⁵ cells / well) and CO (5) concentrations of 5% (CO ₂ ) Incubated for 24 hours at 37 ℃ in ₂ incubator. At this time, normal fibroblasts of humans were used to separate the skin tissue collected after circumcision of newborn in hospital.

DMEM medium containing no serum containing beta-rapacone and retinol at appropriate concentrations was prepared, replaced with this medium, and cultured for 24 hours, followed by cell culture. Procollagen type produced in cell cultures collected using a collagen protein measurement kit from Takara Shuzo Co., Ltd. Japan and an elastin protein measurement kit from Biocolor Co., Ltd. UK. The degree of promoting collagen and elastin production was confirmed by measuring the amount of C-peptide and elastin of I, and the results are shown in Table 1 below.

Composition (concentration) Cell viability
(opponent%) Collagen Type I Content (relative%) Elastin content (relative%) No treatment 100.0 100.0 100.0 Retinol 10 ^-5 M 66.1 - - Retinol 10 ^-6 M 103.1 135.7 109.3 Retinol 10 ^-7 M 100.7 108.5 101.4 Beta-Rapacon 2 ppm 71.6 - - Beta-Rapacon 1 ppm 96.4 145.4 141.6 Beta-Rapacon 0.1 ppm 102.1 143.9 137.3 Beta-Rapacon 0.01 ppm 101.5 110.6 102.0

Referring to Table 1, it can be seen that the application of beta-rapacone according to the present invention shows an excellent collagen and elastin-producing effect equivalent to that of retinol. In particular, when the concentration of beta- rapacon is 0.1 ppm collagen and elastin production rate was confirmed to be significantly improved compared to retinol.

Experimental Example  2: wrinkle improvement effect

In order to confirm the anti-wrinkle effect of the beta-rapacone according to the present invention, a sensory test was performed after the preparation of the cream formulation.

A cream prepared with the composition of Formulation Example 3 below for 20 adult women aged 30 to 60 with fine wrinkles and coarse wrinkles was applied to both sides of the face twice a day for 8 weeks. Derma Top-blue (Breuckmann Corporation, Germany) was measured with various parameters before and after using the product using a variety of parameters, the results obtained are shown in the table below.

Cut elasticity (Courage-Khazaka electronic GmbH, Germany) was measured before and after use of the skin elasticity change was measured, the results obtained are shown in Tables 2 and 3.

In this case, the retinol-containing cream used as a comparative example was replaced with retinol 2500 IU (750 ug / g, 0.075%, KFDA content) instead of beta-rapacone 0.0001% in the composition of Formulation Example 3 below.

<3D roughness parameters>

-Spt: The highest height and lowest valley distance among the folds of the crease

-Spa: average distance between high and low bends

-Sdev: The area when the wrinkle area is flat when the analysis area is 1

Sptm: average distance between peaks and valleys (sum of the 5 highest peaks and 5 lowest valleys)

parameter Before use After 8 weeks of use Example (beta-rapacone containing cream)
Spt 0.688 ± 0.326 0.480 ± 0.145 Spa 0.036 ± 0.008 0.032 ± 0.007 Sdev 1.055 ± 0.015 1.051 ± 0.026 Sptm 0.296 ± 0.042 0.288 ± 0.037 Comparative Example (Retinol-containing Cream) Spt 0.647 ± 0.513 0.539 ± 0.171 Spa 0.036 ± 0.006 0.036 ± 0.008 Sdev 1.054 ± 0.011 1.051 ± 0.034 Sptm 0.288 ± 0.054 0.285 ± 0.027

R ₂ parameters (gross elasticity) Before use After 4 weeks After 8 weeks of use Example (beta-rapacone containing cream) 0.712 0.733 0.735 Comparative Example (Retinol-containing Cream) 0.705 0.721 0.725

Referring to Table 2 and Table 3, when the cream containing beta- rapacon according to the present invention is applied to the skin, compared to the existing retinol-containing cream, after 4-8 weeks of use, skin wrinkle improvement, and elasticity increase It can be seen that the efficacy is excellent.

Experimental Example  3: skin irritation

In order to determine the skin irritation of the beta- rapacon according to the present invention was performed as follows.

In this case, the retinol-containing cream used as a comparative example was replaced with retinol 2500IU instead of beta-rapacone 0.0001% in the composition of Formulation Example 3 below.

1) human skin patch test

Objective skin irritation was measured in 20 women in their 20s to 30s who had no previous hypersensitivity to skin irritation and had no skin disease or skin allergy symptoms.

The test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 ± 10, EPITEST, Finland) and then sealed in the forearm inner part of the subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. 1 hour and 24 hours after each removal (1 hour after removal, 24 hours = 24 hours, 48 hours including patch attachment) using a magnifying glass (8MC-150, DAZOR, United States of America) to observe the test site for erythema and edema Was observed.

At this time, the skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (Table 4), the average skin reactivity was calculated according to Equation 1 below, and the obtained results are shown in Table 5.

Evaluation criteria and score of skin reaction sign Symptom score - Non-responsive, normal skin 0 + - Faint or light erythema 0.5 + Boundary but weak erythema, edema and papules One ++ Pronounced erythema, papules and vesicles 2 +++ Severe erythema and vesicle formation 3 ++++ Cannon formation 6

24h 48h Skin reactivity ± + ++ ± + ++ Control (base cream) - - - - - - 0.00 Comparative Example (Retinol-containing Cream) 5 One - 2 One - 2.29 Example (beta rapacon containing cream) - - - - - - 0.00

Referring to Table 5, the cream containing beta- rapacone according to the present invention can be seen that the skin reactivity is significantly less than about 11 times compared to the retinol cream is significantly less skin irritation.

2) irritation test

Subjective skin irritation was measured in 20 women in their 20s to 40s who had no previous hypersensitivity to skin irritation and had no skin disease or skin allergy symptoms.

After sufficient sweating for 15 minutes using a steam generator, the cosmetic composition was strongly rubbed around both the nose and the cheeks on both sides of the face, and then subjective skin reactions appearing on the face were observed for 10 minutes. However, Psoriasis, Eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, and antihistamines, were excluded from this study.

After applying cosmetics, the score was described in units of 1 minute according to the time when the stimulus response appeared, and the average skin reactivity was calculated by the following Equation 2, and the obtained results are shown in Tables 6 and 7.

Number of positive subjects Sum of reactivity Skin reactivity Control (base cream) 5 17 0.71 Comparative Example (Retinol-containing Cream) 16 113 4.71

Number of positive subjects Sum of reactivity Skin reactivity Control (base cream) 4 19 0.79 Example (beta-rapacone containing cream) 5 13 0.54

Referring to Tables 6 and 7, it can be seen that the cream containing beta- rapacon according to the present invention has a significantly lower skin irritation by about 9 times than the conventional retinol cream.

Experimental Example  4: Cosmetics  Formulation Stability Test

In order to determine the stability of the beta- rapacon according to the present invention was performed as follows.

In this case, the retinol-containing cream used as a comparative example was replaced with retinol 2500IU instead of beta-rapacone 0.0001% in the composition of Formulation Example 3 below.

The cream containing beta-rapacone and the cream containing retinol are stored in an opaque glass container for 1-4 weeks in a constant temperature bath at 45 ° C., kept in a opaque glass container at 4 ° C. and completely After storing for 1 to 4 weeks in a shaded refrigerator and quantified by high pressure liquid chromatography (HPLC) method.

Content measurement result (% control) Week 0 1 week 2 weeks 3 weeks 4 Weeks 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ Comparative example
(Retinol-containing cream) 100 100 95.1 62.5 92.3 58.4 91.4 38.2 90.4 22.7 Example
(Beta-rapacone-containing cream) 100 100 99.9 99.8 99.9 99.6 99.5 99.5 99.5 99.5

Referring to Table 8, the cream containing beta- rapacon according to the present invention, it was shown that the formulation stability is superior to the conventional retinol cream.

Hereinafter, preferred formulation examples are provided to aid the understanding of the present invention. However, the following formulation examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the formulation examples.

Formulation example  1: Softener (Skin Lotion)

As shown in Table 9 below, the flexible longevity containing beta-rapacone was prepared according to a conventional method.

ingredient Content (% by weight) Beta-Rappacon 0.0001 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 ethanol 10.0 Octyldodec-16 0.2 Polysorbate 20 0.2 incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Balance Sum 100

Formulation example  2: convergence makeup

As shown in Table 10 below, the convergent longevity containing beta-rapacone was prepared according to a conventional method.

ingredient Content (% by weight) Beta-Rappacon 0.0001 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-Panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Balance Sum 100

Formulation example  3: nutrition cosmetics

As shown in Table 11 below, nutrient-containing longevity containing beta-rapacone was prepared according to a conventional method.

ingredient Content (% by weight) Beta-Rappacon 0.0001 Glyceryl Stearate SE 1.5 Cetearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 incense 0.01 Uniside-U 13 0.02 Green # 3 0.003 Distilled water Balance Sum 100

Formulation example  4: nutrition cream

Nutritional cream containing beta- rapacone was prepared according to a conventional method as shown in Table 12 below.

ingredient Content (% by weight) Beta-Rappacon 0.0001 Lipophilic monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Balance Sum 100

Formulation example  5: Massage  cream

A massage cream containing beta-rapacone was prepared according to a conventional method as shown in Table 13 below.

ingredient Content (% by weight) Beta-Rappacon 0.0001 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isostearate 5.0 Squalane 5.0 Mineral oil 35 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Balance Sum 100

Formulation example  6: essence

Essences containing beta- rapacon were prepared according to a conventional method as shown in Table 14 below.

ingredient Content (% by weight) Beta-Rappacon 0.0001 glycerin 10 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethylcellulose 0.1 Sodium hyaruronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodec-16 0.4 ethanol 6.0 incense 0.02 Uniside-U 13 0.01 Green # 3 0.001 Distilled water Balance Sum 100

Formulation example  7: pack

A pack containing beta-rapacone was prepared according to a conventional method as shown in Table 15 below.

ingredient Content (% by weight) Beta-Rappacon 0.0001 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-Panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Balance Sum 100

Formulation example  8: ointment

To the ointment containing beta- rapacon as shown in Table 16 was prepared according to a conventional method.

ingredient Content (% by weight) Beta-Rappacon 0.0001 Purified water Remainder Cetostearyl alcohol 3.0 Hard flow paraffin 5.0 Tocopherol acetate 2.0 Seteareth-20 3.0 Panthenol 0.5 Methyl paraoxybenzoate Quantity Paraoxybenzoic Acid Profiles Quantity

Claims (7)

Skin external composition for promoting collagen biosynthesis comprising beta-rapacone represented by Formula 1 as an active ingredient:
[Formula 1]

According to claim 1, wherein the composition is a topical composition for promoting collagen biosynthesis, characterized in that containing beta- rapacone in an amount of 0.00001 to 10.0% by weight based on the total weight of the composition. The external preparation composition for promoting collagen biosynthesis according to claim 1, wherein the external preparation composition for promoting collagen production is an ointment, gel, cream, patch or spray formulation. A cosmetic composition for improving wrinkles comprising beta-rapacone represented by Formula 1 as an active ingredient:
[Formula 1]

The cosmetic composition for improving wrinkles according to claim 4, wherein the beta-rapacone has a collagen synthesis promoting effect. According to claim 4, wherein the composition is wrinkles cosmetic composition for improving wrinkles, characterized in that it comprises beta- rapacon in an amount of 0.00001 to 10.0% by weight based on the total weight of the composition. The cosmetic composition for improving wrinkles according to claim 4, wherein the cosmetic composition for improving wrinkles is one selected from the group consisting of lotion, milky lotion, cream, foam, essence, serum, softening water, milky lotion, foundation, makeup base, soap, sunscreen cream and sun oil. Wrinkle improvement cosmetic composition, characterized in that having a formulation.