KR20110078365A - Composition of skin external application for slimming containing ginseng berry extracts - Google Patents

Abstract

PURPOSE: An external use skin composition for sliming containing ginseng berry extract is provided to promote fat decomposition and to reduce cellulite. CONSTITUTION: An external use skin composition for sliming contains 0.0001-50 weight% of ginseng berry extract as an active ingredient. The ginseng berry extract is prepared by removing seeds from ginseng berries, drying the ginseng berries by the sunlight or hot wind, and isolating by water or ethanol. The composition is manufactured in the form of skin softener, lotion, massage cream, pack, cream, gel, or ointment.

Description

Composition of skin external application for slimming containing ginseng berry extracts}

The present invention relates to a slimming skin external preparation composition containing ginseng fruit extract, and more specifically, by containing the ginseng fruit extract having a unique ingredient and composition as the active ingredient in the ground portion of ginseng as an active ingredient, subcutaneous lipolysis effect of cell and cellulite It relates to a skin external composition for slimming excellent in the removal effect of.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi ginseng and has been used in Korea, China, and Japan for more than 2,000 years, and has been used to prevent disease and extend life. Known efficacy and effects of ginseng to the central nervous system, anticarcinogenic effect, anticancer activity, immune function regulation, antidiabetic effect, liver function anti-inflammatory effect, cardiovascular disorders improvement, anti-arteriosclerosis, blood pressure control action, menopausal Disability improvement, effects on osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effect (Latest ginseng 'components and potency', Korea Ginseng and Tobacco Research Institute, 56-112, 1996).

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground parts of ginseng. Especially, ginsenoside content and composition are different depending on the parts such as ginseng root (root), ginseng leaf and ginseng fruit. (Attele AS et al, Biochem Pharmacol, 58; 1685-1693, 1999).

Recently, as consumers' interest in natural cosmetics has increased and many cosmetics using herbal ingredients have been released, ginseng is also being studied as a plant material having important skin effects of cosmetics, but most of them are extracts or ginseng roots of ginseng root. Only the side ingredients and ginseng polysaccharides utilizing the slimming effect of the components of the ginseng fruit is a situation that does not exist.

In addition, ginseng fruit is considered more precious than ginseng since ancient times, and has been selected and harvested for the purpose of obtaining seeds. Ginseng fruit is harvested only once in 4 years of ginseng cultivation period. It is difficult to produce good seedlings because seed is too small when harvested in 3 years old. Seeds are faithful and good when grown in 5 years old or older. In addition to inhibiting development, the tissue is not dense, so red ginseng quality may be greatly reduced when manufacturing red ginseng. In addition, the number and frequency of red ginseng deterioration is greatly reduced when the number of crops is collected more than two times during the cultivation period.

There are about 20 billion fat cells in the human body, and it is responsible for accumulating or releasing energy in mammals. Within these cells, there are complex regulatory principles of energy accumulation and release, which are stored as triglycerides in adipocytes when the supply is much greater than the demand for energy, and then broken down into free fatty acids and glucose when energy is depleted. It is used. Obesity is caused by excessive energy accumulation due to the imbalance of this process, and is believed to be due to the increase or increase in the size of fat cells.

Women's interest in a beautiful body is rapidly increasing not only due to the increase in obesity caused by abundant life, but also due to the improvement of social status, economic independence, and the quality of life of the woman, thus removing excessive subcutaneous fat and skin elasticity. There is also a growing interest in slimming and anti-celullite cosmetics that are effective in promoting.

Therefore, the present inventors have extensively researched to develop a safe lipolytic active ingredient while promoting lipolysis and assisting in discharging fat cells, and the ginseng fruit extract has a different composition and composition than the general ginseng and red ginseng. Due to the excellent effect of promoting the breakdown of triglycerides in fat cells when applied to the skin has been found to reduce the size of fat cells, reduce the thickness of subcutaneous fat and reduce the cellulite, to complete the present invention.

Accordingly, it is an object of the present invention to provide a skin external composition having an excellent effect of promoting the breakdown of fat cells and reducing cellulite using a natural material safe for the human body.

In order to achieve the above object, the present invention provides a skin external composition for slimming containing ginseng fruit extract as an active ingredient.

In the present invention, the external composition for skin containing a ginseng fruit extract having a different ingredient and composition from ginseng root as an active ingredient in the ground part of ginseng promotes the process of decomposing triglycerides accumulated in adipocytes into glycerol and free fatty acids and releasing them. Not only does it reduce the subcutaneous fat layer, it can also make the skin smooth and smooth by reducing the cellulite that causes bumpy skin.

The present invention relates to a slimming skin external composition for containing a ginseng fruit extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The ginseng fruit extract according to the present invention is prepared by extracting the flesh and skin of the ginseng fruit separated and separated from the seeds by sun drying or hot air drying, followed by extraction with water or ethanol.

The external skin composition according to the present invention is a skin external composition which is effective in degrading neutral fat by reducing ginseng fruit extract and reducing cellulite to make a slim body, and thus a slimming effect of decomposing subcutaneous fat when administered as a skin formulation. Is very good.

The ginseng fruit extract of the present invention may be mixed in an amount of 0.0001 to 50% by weight based on the total weight of the composition, depending on the form of the skin external preparation composition. If it is less than 0.0001% by weight, the effect is insignificant, and if it exceeds 50% by weight, the formulation stability is deteriorated, which is not preferable.

The external preparation composition for skin according to the present invention is not particularly limited in its formulation as long as it is intended to give a breakdown of fat and elasticity, beauty and suppleness of female skin. Specifically, for example, it may be a cosmetic composition having a formulation of softening lotion, nourishing lotion, nourishing lotion, massage cream, nourishing cream, pack, gel or skin adhesive type cosmetics, and also includes lotion, ointment, gel, cream, It may be a transdermal dosage form such as a patch or spray.

In addition, in the composition for external application for skin of each formulation, other components other than the ginseng fruit extract, which is an essential ingredient of the present invention, may be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other external preparation for skin. If applied simultaneously with other raw materials, synergistic effects may occur.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples and Test Examples. However, these experimental examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope and scope of the present invention.

Preparation Example 1 Preparation of Ginseng Fruit Extract

1) Ginseng fruit pretreatment: Raw ginseng fruit was harvested, seed was separated and removed, and then the ginseng fruit dried raw material was prepared by sun drying or hot air drying.

2) Preparation of ginseng fruit extract: 3 g of water was added to 1 kg of dried ginseng fruit extract to extract reflux, and then filtered and concentrated under reduced pressure at 40 to 45 ° C. to obtain 300 g of ginseng fruit extract.

Preparation Example 2 Preparation of Ginseng Root Extract

Except for using ginseng root (root) instead of ginseng fruit in Preparation Example 1 was prepared in the same manner as in Preparation Example 1.

Test Example 1 Comparison of Ingredients of Ginseng Fruit Extract

<Ginsenoside (Ginseng Saponin) Analysis of Ginseng Fruit and Ginseng Root>

Ginseng fruit and ginseng root extract were prepared in Preparation Examples 1 to 2, respectively, and these extracts were treated with ether to remove fat-soluble components, followed by extracting and concentrating ginsponin with butanol (BuOH). Side component analysis was performed and the results are shown in Table 1.

Preparation Example 1 Production Example 2 Irradiation Ponine Content
(Dry weight) 33.42% 16.70% PD / PT ratio 0.73 3.23

The ginseng fruit extract prepared in Preparation Example 1 had about twice the content of the ginseng root extract prepared in Preparation Example 2 with respect to the content of irradiated saponin. And Rc and Rd "and PT (Protopanaxatriol)-" Ginsenoside Re, Rg1 and Rg2 "ratios were 0.73 and 3.23, respectively. .

Mineral Analysis of Ginseng Fruit Extract

In order to distinguish that the ginseng fruit extract prepared in Preparation Example 1 has the characteristics of 'fruit' different from ginseng, mineral components including vitamins were analyzed, and the results are shown in Table 2 below.

ingredient content ingredient content Potassium (mg / 100g) 5865.57 Magnesium (mg / 100g) 354.38 Calcium (mg / 100g) 819.26 Zinc (mg / 100g) 178.49 Iron (mg / 100g) 59.31 Vitamin A (µg / 100g, RE) 213.11 Phosphorus (mg / 100g) 187.17 Vitamin B1 (mg / 100g) 12.29 Vitamin B2 (mg / 100g) 8.45 Vitamin B6 (mg / 100g) 10.50 Vitamin C (mg / 100g) 4.91 Vitamin E (mg / 100g, a-TE) 23.61 Vitamin K (μg / 100g) 232.12 Niacin (mg / 100g, NE) 5.76 Pantothenic Acid (mg / 100g) 5.87 Folic Acid (µg / 100g) 349.97

As described above, the ginseng fruit used in the present invention contains the ginseng saponin more than the ginseng root, and at the same time, the composition of the saponin is opposite to the ginseng root. It was confirmed that, based on this, the following experiments on the skin efficacy was carried out.

[Test Example 2] Ginseng Fruit Extract Promotes the Degradation of Triglycerides in Adipose Cells

3T3-L1 cells, a mouse fibroblast cell line, were loaded with DMEM (Dulbecos modified eagles medium, GIBCO BRL, Life Technologes) medium containing 10% fetal bovine serum (FBS). The well culture plate was attached at 1 × 10 ⁵ cells / well. After 2 days, it was replaced with fresh DMEM (containing 10% FBS) medium and incubated for 2 days. The incubated cells were then induced to differentiate with DMEM (containing 10% FBS) containing 1 μg / ml insulin, 0.5 mM IBMX and 0.25 μM dexamethasone, and again after 2 days. Incubated for 5 days by exchange with DMEM containing insulin. After 5 days, the cells were exchanged with normal medium (DMEM, containing 10% FBS) and cultured while observing until the cells changed to fat cells.

In order to evaluate the effect of ginseng fruit extract on the promotion of triglyceride breakdown in adipocytes, experiments were performed using the 3T3-L1 adipocytes that have been differentiated. 3T3-L1 adipocytes were washed twice with PBS (phosphate buffered saline), and colorless DMEM containing 0.5% bovine serum albumin (BSA) without fatty acids was added and used in each experiment. As a control, only the medium without the test substance or the comparative substance was used, and when the result was 100%, other values were expressed. In addition, 0.1% of caffeine was treated with another control group. The degree of lipolysis was determined by measuring the concentration of glucose released from adipocytes into the culture. Glucose was measured by color reaction method using GPO-trinder kit purchased from Sigma Co., Ltd. (St. Louis, MO, U.S.A.), and absorbance was measured at 540 nm using ELISA reader. The results are shown in Table 3.

division Control group Caffeine
0.1% Ginseng Fruit Extract
(Manufacture example 1) 0.01% Ginseng Root Extract
(Manufacture example 2) 0.01% Amount of free glycerol 100% 167% 232% 120%

As shown in Table 3, the group treated with ginseng fruit extract was found to significantly increase the concentration of glucose released from the adipocytes into the culture medium compared to the control group. In particular, the group treated with the ginseng fruit extract according to the present invention showed an excellent lipolysis effect than the treated group of the ginseng root extract, and also has a better lipolysis effect than when treated with caffeine used as a positive control. It can be seen.

Test Example 3 Slimming Effect of Ginseng Fruit Extract

In order to confirm the slimming effect of the ginseng fruit extract was prepared a slimming lotion according to the composition of Table 4.

ingredient Example 1 (% by weight) Comparative Example 1 (% by weight) Purified water To 100 To 100 Ginseng Fruit Extract (Manufacturing Example 1) 1.0 - Ginseng Root Extract (Manufacturing Example 2) - 1.0 Vegetable hydrogenated oil 1.50 1.50 Stearic acid 0.60 0.60 Polyglycerol-10 Pentastearic & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerol Olate & Propylene Glycol 1.50 1.50 Caprylic / Carlic Triglycerides 4.00 4.00 Meadowfoam fruit oil 3.00 3.00 Cetyl octanoate 4.00 4.00 Cyclomethicone 6.00 6.00 Methyl paraben 0.20 0.20 Profile paraben 0.10 0.10 Disodium EDTA 0.02 0.02 Trimethanolamine 0.13 0.13 glycerin 8.00 8.00 Carbomer 0.13 0.13

40 women aged 25 to 46 with BMI (body mass index, body weight (kg) / height (m) ² ) who are either obese or cellulite, morning and evening for four weeks. Twice at one thigh at home with the user's massage lotion of the formulation was used for 8 weeks before and after use was evaluated by device evaluation, evaluation by the investigator (dermatologist) and questionnaire evaluation.

Ultrasound-EuB 415 US scanner was used for the measurement of the thickness of subcutaneous fat layer using ultrasound, and the obtained value was a bilateral test using the Student t test or Wilcoxon test. (Significance level α = 0.05). The results are shown in Table 5.

Visual evaluation by the investigator was evaluated by the elasticity and the degree of cellulite. The obtained values were analyzed by the Wilcoxon test using the Wilcoxon test. The statistical significance was analyzed by using the Wilcoxon test (significance level α = 0.05). The elasticity was evaluated in nine stages from the lowest to the highest nine points, and the degree of cellulite was evaluated in five stages from zero to four points by visual evaluation. The results are shown in Table 6.

division Example 1 Comparative Example 1 % Decrease in subcutaneous fat thickness 2.8 0.2

As can be seen in Table 5, when using Example 1 to add the ginseng fruit extract compared to Comparative Example 1 showed a 2.8% decrease in thigh circumference showed a statistically significant result. At this time, the weight of the subject did not change during the test period.

division Example 1 Comparative Example 1 % Reduction in cellulite 20 3 % Increase in elasticity 15 2

As can be seen in Table 6, the cellulite reduction (20%) and skin elasticity increase (15%) was statistically significant at the site of Example 1 compared to Comparative Example 1 in the efficacy evaluation by the researcher.

Therefore, the composition containing the ginseng fruit extract of the present invention exhibited an excellent slimming effect by effectively reducing subcutaneous fat and cellulite without causing a decrease in elasticity of the skin.

Claims (4)

Slimming skin external composition containing ginseng fruit extract as an active ingredient. The composition for external application for skin according to claim 1, wherein the ginseng fruit extract is contained in an amount of 0.0001 to 50% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the composition breaks down triglycerides. The composition for external application for skin according to claim 1, wherein the composition removes cellulite.