KR101757790B1 - Composition of skin external application containing Panax ginseng var. Hwangsookjong extracts - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing ginseng extract, and more particularly, to a composition for external application for skin containing ginseng extract of Hwang Sookjong, which comprises a ginseng extract of Hwangsinjong ginseng, a variant of ginseng, The present invention relates to a composition for external application for skin having an effect of inhibiting MMP-1 and simultaneously inhibiting skin aging and improving wrinkles through antioxidative and DNA damage protection effects. In addition, the composition for external application for skin according to the present invention exhibits skin whitening effect by inhibiting melanin formation and improving pigmentation caused by ultraviolet rays, and inducing and maintaining the differentiation of dermal keratinocytes to maintain skin dryness symptoms and atopic symptoms It is also effective in controlling sebum secretion and improving acne and skin troubles through anti-inflammatory effect.

Description

{Composition of a skin external application containing Panax ginseng var. Hwangsookjong extracts}

The present invention relates to a composition for external application for skin containing ginseng extract, and more particularly, to a composition for external application for skin containing ginseng extract of Hwang Sookjong, which comprises a ginseng extract of Hwangsinjong ginseng, a variant of ginseng, The present invention relates to a composition for external application for skin having an effect of inhibiting MMP-1 and simultaneously inhibiting skin aging and improving wrinkles through antioxidative and DNA damage protection effects. In addition, the composition for external application for skin according to the present invention exhibits skin whitening effect by inhibiting melanin formation and improving pigmentation caused by ultraviolet rays, and inducing and maintaining the differentiation of dermal keratinocytes to maintain skin dryness symptoms and atopic symptoms It is also effective in controlling sebum secretion and improving acne and skin troubles through anti-inflammatory effect.

Ginseng (Panax ginseng C.A. Meyer) is a herb that belongs to the genus Ogawa and Ginseng. It has been used in Korea, China and Japan for over 2,000 years and has been used empirically to prevent disease and prolong the life span. The efficacy and effect of ginseng which has been known so far can be determined by the action of the central nervous system, the anticarcinogenic action, the anticancer activity, the immune function regulating action, the anti-diabetic action, the hyperfunction of liver function, the improvement of cardiovascular disorder, (Korean Ginseng & Tobacco Research Institute, 56-112, 1996). In addition, it has been reported that the antioxidant activity and antioxidant activity of osteoporosis are improved.

Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. Especially, ginsenoside (root), ginseng leaf and ginseng berries have not only ginsenoside content but also composition (Attele AS et al, Biochem Pharmacol, 58: 1685-1693, 1999).

The ginseng of Panax ginseng contains four varieties of japonica var. Jakeungjong, hwangsookjong, var. Hongsookjong, var. Chungkeangjong, There is an equinox (黄黄 熟 種, var. Deunghwangsookjong).

Hwang sookjong is characterized by yellow fruit at the time of fruit production, and it is firstly found in Ginseng, Jangdan County, Gyeonggi Province in 1926 and 1928, and is cultivated in some farms.

Hwang sookjong has a higher survival rate and a higher survival rate per unit area because it is more resistant to infectious diseases than its own. In addition, the quality of red ginseng is higher than that of the wild species. (Kwon, Y. et al., Korean J. Breed. 26 (4): 400-404 (1994)). The survival rate of Hwangsookjong was higher than that of other species (Lee, Sungwoo et al., Korean J. Crop Sci., 53 (4): 401-406 (2008)).

Hwang, S., and Hwang, S., Ginseng Sci., Vol. 13, no. 2, p. 153-157 (1989)).

Recently, consumers' interest in natural cosmetics has been increasing, and at the same time, many cosmetics utilizing herbal materials have been released, and ginseng has been studied as a plant material having important skin effect of cosmetics.

However, there are not many raw materials which are known as skin-safe natural materials to date, which approach and reduce the fundamental cause of sebum secretion and alleviate the inflammatory action.

Therefore, the present inventors have found that the Hwang sookjong extract, which is a variant of Korean ginseng, is superior in skin-improving effect due to a composition different from that of common ginseng ginseng as a result of efforts to find a skin-safe substance having various skin- Thereby completing the present invention.

Accordingly, an object of the present invention is to provide a composition for external application for skin for preventing skin aging or improving wrinkles, which has an antioxidative effect, a collagen production promotion effect or an MMP-1 inhibitory effect.

It is also an object of the present invention to provide a composition for external application for skin whitening which has an effect of inhibiting melanin formation and improving pigmentation.

It is another object of the present invention to provide a composition for external application for skin moisturizing or atopic symptom improvement which has skin moisturizing effect and atopic symptom improvement effect inducing and maintaining normal differentiation of epidermal keratinocytes.

It is also an object of the present invention to provide an anti-inflammatory external preparation for skin which can suppress sebum control or anti-inflammatory effect, that is, the production of an inflammatory factor, thereby improving skin troubles including acne.

In order to attain the above object, the present invention provides a composition for external application for skin, which comprises Hwang Sookjong ginseng extract as an active ingredient.

The composition for external application for skin according to the present invention contains the ginseng extract having different composition from the ginseng species as an active ingredient among the varieties of Korean ginseng, thereby suppressing skin aging, improving wrinkles, improving whitening, moisturizing and atopic symptoms, improving acne and skin troubles .

The present invention relates to a composition for external application for skin, which is prepared by mixing Hwang sookjong ginseng extract in an amount of 0.001 to 50 wt% based on the total weight of the composition according to the form of the composition for external application for skin.

The ginseng extract used in the present invention is prepared by extracting the flesh and the skin of the fruit which has been separated or removed from roots or seeds of the ginseng root of Ginseng root with sunlight or hot air, followed by extraction with water or ethanol.

The present invention provides a composition for external application for skin inhibition or wrinkle suppressing skin having antioxidative effect, collagen production promotion or MMP-1 inhibitory effect by containing Hwang sookjong ginseng extract as an active ingredient.

In addition, the present invention provides a composition for external skin application for skin whitening which has an effect of inhibiting melanin formation or improving pigmentation by containing the above-mentioned ginseng extract as an active ingredient.

The present invention also provides a composition for external application for skin moisturizing or atopic symptom improvement, which has skin moisturizing effect and atopic symptom improvement effect, which induces and maintains normal differentiation of epidermal keratinocytes by containing the above-mentioned ginseng extract as an active ingredient.

In addition, the present invention provides an anti-inflammatory skin topical composition for anti-inflammation which can improve sebum control or anti-inflammatory effect, that is, the production of an inflammatory factor, thereby improving skin troubles including acne by containing the ginseng extract as an active ingredient.

The composition for external application for skin according to the present invention may further contain a cosmetically or dermatologically acceptable medium or base conventionally used in the art. It may be any form suitable for topical application according to conventional methods in the art, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water phase, a suspension, a microemulsion, a microcapsule, Or in the form of ionic (liposomes) and non-ionic follicular dispersants, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. The composition for external application for skin according to the present invention may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The composition for external application for skin according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Any other ingredient commonly used in cosmetics, lipid vesicles or cosmetics may be added to the composition, including, but not limited to, nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, , And the adjuvants may be introduced in amounts commonly used in the cosmetics or dermatology fields.

Further, the composition for external application for skin according to the present invention is not particularly limited in its formulation, and examples thereof include softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, Foam, cleansing water, pack, powder, body lotion, body cream, body oil or body essence.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. It is to be understood that the scope of the present invention is not limited to these embodiments and test examples, and that variations, substitutions, and insertions conventionally known in the art And this is also included in the scope of the present invention.

[Example 1] Preparation of Hwang sookjong ginseng extract

1) Hwang Sook Jong Ginseng Root Pretreatment: Hwang Sook Jong raw ginseng was harvested to remove soil, and roots were dried with sunshine or hot air to produce dried ginseng roots.

2) Preparation of Hwang sookjong ginseng root extract: To 1 kg of Hwang sookjong ginseng root dried product, 3 L of water was added, and the mixture was refluxed. After filtration, the filtrate was concentrated under reduced pressure at 40~45 ℃ to obtain 300 g of the extract.

3) Hwang Sook Jong Ginseng Fruit Pretreatment: Hwang Sook Jong Fresh ginseng fruit was harvested and seeds were separated and removed. Then, the flesh and perilla of the fruit were dried by sunlight or hot air drying to prepare dry matter of ginseng root.

4) Preparation of Hwang Sookjong Ginseng Fruit Extract: Hwang Sookjong 3 l of water was added to 1 kg of dried ginseng fruit, and the mixture was refluxed and filtered. The mixture was concentrated under reduced pressure at 40-45 ° C to obtain 300 g of Hwang Sookjong ginseng fruit extract.

[Comparative Example 1] Manufacture of ginseng extract

In the same manner as in Example 1, except for using Ganoderma ginseng (Gamyeong-myeon, Jinan-gun, Jeollabuk-do) instead of Ganoderma ginseng in Example 1, root ginseng root and fruit extract were prepared.

[Test Example 1] Comparison of components of Hwang sookjong and ginseng extract

<Analysis of Ginsenosides (Ginseng Saponin) Components of Hwangsookjong and Ginseng Root and Fruits of Ginseng>

The extracts prepared in Example 1 and Comparative Example 1 were treated with ether to remove fat-soluble components, crude saponin was extracted with butanol (BuOH), concentrated, and analyzed for ginsenoside components by HPLC. The results are shown in Table 1 below.

Compare Example 1 Root Example 1 Fruit Comparative Example 1 [ Comparative Example 1 Fruit Crude saponin content
(Dry weight) 16.20 33.12% 14.70% 31.42%

From the results shown in the above Table 1, it was confirmed that the crude saponin content of the Hwang SJ type ginseng roots and the Fruit extracts prepared in Example 1 was about 1 to 1.1 times that of the root ginseng roots and the fruit extract prepared in Comparative Example 1, Respectively.

[Test Example 2] Effect of suppressing skin aging and improving wrinkles

1) Antioxidative Effect of Ginseng Root and Fruit Extract

The antioxidant effect was investigated by comparing the ability to remove reactive oxygen species (ROS) from cells by ultraviolet (UV) irradiation. As a positive control group, trolox, which is generally used to compare antioxidative effects, was used. In addition, the test group was not used as a control group.

Human HaCaT keratinocytes isolated from human epidermal tissue were added to each well of a 24 well plate in an amount of 5 x 10 ⁴ and adhered for 24 hours. After 16 hours, each test substance was treated with 10 [mu] g / ml. Two hours later, the culture medium was removed, and 100 占 퐇 of PBS was added to each well. The keratinocytes were irradiated with ultraviolet rays of 30 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15 W, Sankyo Dennki, Japan), and PBS was removed. 200 [mu] l was added. The same test material was treated again and the amount of active oxygen species increased by ultraviolet stimulation after 3 hours was quantified. The amount of ROS was determined by referring to Tan's method of measuring the fluorescence of DCF-DA (dichlorofluorescin diacetate) oxidized by ROS (Tan et al., 1998, J. Cell Biol. Vol. 141, pp1423-1432) The inhibition rate of ROS production was calculated using the following equation (1).

Test substance ROS generation inhibition rate (%) Control group 0 Trolox 40 Example 1 Root 3 Example 1 Fruit 27 Comparative Example 1 [ 4 Comparative Example 1 Fruit 37

From the results shown in the above Table 2, it can be seen that the roots and fruit extracts of the ginseng root of Example 1 according to the present invention are more effective than those of Comparative Example 1 in the human HaCaT keratinocytes monolayer culture system It was confirmed that oxygen was significantly erased. This activity was similar to that of trolox used as an activity index of antioxidants. In other words, it was confirmed that the extract of Root and Ginseng according to Example 1 of the present invention has the effect of preventing wrinkle formation, elasticity degradation and pigment deposition by significantly erasing active oxygen which is a cause of skin aging Respectively.

2) Inhibitory activity against DNA oxidative damage by single cell gel electrophoresis (Comet assay)

After human normal fibroblasts were cultured for 24 hours, the extracts of Example 1 and Comparative Example 1 and vitamin E were treated for each concentration, and 12 hours after stimulation, H ₂ O ₂ (final concentration: 10 ^-3 M) was treated The results of staining with the following electrophoresis were observed with a fluorescence microscope. At this time, the length (쨉 m) of the tail showing the result of DNA cleavage in each of 25 cells was analyzed using an image analyzer, KOMET 3.1 (Kinetic Imaging, England). The results are shown in Table 3 below .

Inhibitory Effects of Ginseng Root and Fruit Extract, Vitamin E on DNA Oxidative Damage Test substance Tail length (탆) Inhibition rate (%) H ₂ O ₂ 115.6 - H ₂ O ₂ + Example 1 Root
10 ppm 80.1 30.7 50 ppm 69.4 39.9 100 ppm 56.2 51.4 H ₂ O ₂ + Example 1 Fruit
10 ppm 76.8 33.6 50 ppm 66.2 42.7 100 ppm 52.8 54.3 H ₂ O ₂ + Comparative Example 1 Root
10 ppm 85.1 26.4 50 ppm 72.7 37.1 100 ppm 61.0 47.2 H ₂ O ₂ + Comparative Example 1 Fruit
10 ppm 80.8 30.1 50 ppm 69.8 39.6 100 ppm 57.4 50.3 H ₂ O ₂ + Vitamin E 10 ppm 70.6 38.9

From the results shown in Table 3, it was confirmed that the root-ginseng roots and the fruit extract of Example 1 by cell gel electrophoresis showed significant oxidative DNA damage-inhibiting activity, and thus, the root- And it acts as a DNA oxidative damage inhibitor that inhibits DNA single strand break by hydroxyl radical (.OH).

3) Type I procollagen assay (Type I Procollagen assay)

The human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then the medium containing the extracts of Example 1 and Comparative Example 1 was replaced with a medium containing 1 and 10 ppm, respectively. On the third day of culture, the cells were harvested and the amount of type I procollagen produced by the ELISA method was quantified. The results are shown in Table 4 below. The result was calculated by comparing the value obtained by measuring the value of the control group containing no test substance of Example 1 as 100. [

Test substance Amount of type I procollagen Control group 100 Example 1 Root
1 ppm 121 10 ppm 130 Example 1 Fruit
1 ppm 119 10 ppm 147 Comparative Example 1 [
1 ppm 123 10 ppm 132 Comparative Example 1 Fruit
1 ppm 122 10 ppm 163

From the results shown in the above Table 4, it was confirmed that the Hwang sookjong ginseng roots and the fruit extract of Example 1 significantly promoted the formation of type I procollagen in the normal human fibroblast monolayer culture system. That is, the ginseng roots and fruit extracts of Example 1 showed wrinkle-reducing effect by inhibiting the reduction of collagen production caused by aging of human skin.

4) MMP-1 expression inhibition assay

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , then irradiated with ultraviolet B at 40 mJ / cm 2, and then replaced with medium containing each test substance in the amount shown in Table 5 below. On the second day of culture, the cells were harvested and the amount of MMP-1 (matrix metalloproteinase-1) produced by ELISA was quantitated. The result was calculated by comparing the value measured with the ultraviolet control group containing no test substance as 100. RA (Retinoic acid; Sigma.) Was used as a positive control group.

Test substance MMP-1 expression level (%) Control group 100 RA 10 μM 50 Example 1 Roots 10 ppm 85 Example 1 Fruit 10 ppm 66 Comparative Example 1 Roots 10 ppm 78 Comparative Example 1 Fruit 10 ppm 61

From the results shown in the above Table 5, in the human normal fibroblast monolayer culture system, the Hwang SJ Root and Fruit Extract of Example 1 according to the present invention significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / cm 2 . Therefore, the roots and fruit extracts of the ginseng root of Example 1 according to the present invention inhibit the biosynthesis of MMP-1, a skin tissue degrading enzyme caused by internal aging or external environmental factors, thereby suppressing skin aging and improving wrinkles Respectively.

5) Effect of inhibiting biosynthesis of cyclooxygenase-2 (COX-2) by ultraviolet light

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then irradiated with ultraviolet ray A at 15 J / cm 2. Then, the roots and fruit extracts of the roots of the roots of Example 1 and the roots of the roots of the roots of ginseng and the fruit extract Were replaced with medium containing 0.1, 1 and 10 ppm, respectively. On the second day of culture, the cells were harvested and the amount of cyclooxygenase-2 produced was quantified using the Westren blot method. The results are shown in Table 6 below. The results are shown in Table 6 below. [Table 6] &lt; EMI ID = 16.1 &gt;

Test substance COX-2 biosynthesis (%) Example 1 Root 10 ppm 61 1 ppm 76 0.1 ppm 92 Example 1 Fruit 10 ppm 21 1 ppm 50 0.1 ppm 74 Comparative Example 1 [ 10 ppm 69 1 ppm 82 0.1 ppm 97 Comparative Example 1 Fruit 10 ppm 31 1 ppm 59 0.1 ppm 80 Control group 100

From the results shown in Table 6, the ginseng roots and fruit extracts of Example 1 decreased the biosynthesis of cyclooxygenase-2 by ultraviolet rays in a concentration-dependent manner, and prostaglandin E2 (PGE2), a product of COX- It was confirmed that it prevents the skin tissue degradation by

6) Inhibitory effect of UV-induced tumor necrosis factor (TNF-α) on biosynthesis

Human keratinocytes were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , then irradiated with ultraviolet B at 30 mJ / cm 2, and then replaced with media containing 0.1, 1 and 10 ppm of the extracts of Example 1 and Comparative Example 1, respectively Respectively. After 6-24 hours of culture, the cells were harvested and the amount of TNF- [alpha] produced was quantitated using ELISA (Pharmingen 555212) method. The amount of TNF- [alpha] The results are shown in Table 7 below.

Test substance TNF-α biosynthesis (%) Control group 100 Example 1 Root 10 ppm 55 1 ppm 74 0.1 ppm 87 Example 1 Fruit 10 ppm 29 1 ppm 46 0.1 ppm 72 Comparative Example 1 [ 10 ppm 69 1 ppm 81 0.1 ppm 92 Comparative Example 1 Fruit 10 ppm 36 1 ppm 58 0.1 ppm 79

From the results of Table 7, it can be seen that the roots and fruit extracts of the Ginseng root of Example 1 according to the present invention reduce the biosynthesis of TNF-α by ultraviolet rays in a concentration-dependent manner, thereby preventing skin aging caused by biosynthesis of TNF- And the like.

7) Skin wrinkle improvement effect on human skin

For the 40 subjects who had facial wrinkles of 30 to 50 years old, the nutritional creams of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 and 2 having the compositions shown in the following Table 8 were prepared by a conventional method, (Unit: wt%).

ingredient Formulation Example 1 Formulation Example 2 Comparative Formulation Example 1 Comparative Formulation Example 2 Hwang sookjong ginseng root extract (Example 1) 0.1 - - - Hwang Sookjong ginseng fruit extract (Example 1) - 0.1 - - Ginseng root extract (Comparative Example 1) - - 0.1 - Ginseng fruit extract (Comparative Example 1) - - - 0.1 Wax 10 10 10 10 Polysorbate 60 1.5 1.5 1.5 1.5 Piggy 60 hardened castor oil 2 2 2 2 Sorbitan sesquioleate 0.5 0.5 0.5 0.5 Liquid paraffin 10 10 10 10 Squalane 5 5 5 5 Caprylic / capric triglyceride
(Estasan: Uniqema (manufacturer)) 5 5 5 5 glycerin 5 5 5 5 Butylene glycol 3 3 3 3 Propylene glycol 3 3 3 3 Triethanolamine 0.2 0.2 0.2 0.2 Preservative, coloring, fragrance Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance

Formulation Example 1 or 2 was used for the face side of the subject and Comparative Formulation Example 1 or 2 was used for the right side for 3 months, respectively. The skin condition of both sides of the facial area before the use of the cream was measured, and then the same part was remeasured 3 months after using the cream to measure the change of the skin wrinkles. The skin wrinkles were measured with a Visiometer system (C + K company) by removing the wrinkles of the eye tail region with a replica in a constant temperature and humidity chamber at 24 ° C and a relative humidity of 40%. The amount of change in the skin wrinkles was calculated according to the following formula (2).

In the equation (2), "Tdi" is the measured site value at D90 and "Tdo" is the measured site value at D0.

Test substance Skin wrinkle reduction (%) Formulation Example 1 31 ± 9 Formulation Example 2 39 ± 11 Comparative Formulation Example 1 27.5 ± 7 Comparative Formulation Example 2 35.7 ± 10

In the results of Table 9 above, the skin wrinkle reduction value of 27.5 + 7% (mean + -standard deviation) was shown in the group using Comparative Formulation Example 1, and 35.7 + 10% in Comparative Formulation Example 2, The skin wrinkle reduction value was 31 ± 9% in the used group and 39 ± 11% in the case of Formulation Example 2, and the excellent wrinkle improving effect was confirmed.

8) Skin elasticity improvement effect in human body

The skin elasticity improving effects of the nutritional creams of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 and 2 prepared above were measured. Forty women aged 30 or older were divided into four groups. The nutritional creams of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 and 2 were applied to the face for 12 weeks for 2 weeks each day. Then, the skin elasticity was measured with a cutter SEM 575 , C + K Electronic Co., Germany). The results are shown in Table 10 below. The results in Table 10 indicate the viscoelasticity of the skin elasticity meter.

Test substance Skin elasticity effect Formulation Example 1 0.32 ± 0.08 Formulation Example 2 0.46 0.11 Comparative Formulation Example 1 0.29 + 0.07 Comparative Formulation Example 2 0.40 ± 0.09

In the results of Table 10 above, when the nutritional creams of Formulation Examples 1 and 2 were used, skin elasticity enhancement effect was higher than Comparative Formulation Examples 1 and 2.

[Test Example 3] Skin whitening effect

1) Inhibitory effect of melanin on melanin production in rat

In order to examine the inhibitory effect of the ginseng extract of Example 1 on melanogenesis, mouse pigment cells were used.

First, 10% fetal bovine serum (100 μl) was injected into DMEM (Dulbeccos modified Eagles media) and 100 μl of 100 μl The cells were cultured in the presence of nM 2-O-tetradecanoylphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 ⁵ cells / well (cells / well) on a 24-well plate. The extract of Example 1 was added at 10 ppm / well. At this time, 1 ppm of hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. Then, the inhibition rate of melanin production was calculated according to the following formula (3) (Dooley's method).

Test substance Melanin production inhibition rate (%) Example 1 Roots 10 ppm 25.8 Example 1 Fruit 10 ppm 32.4 Comparative Example 1 Roots 10 ppm 29.1 Comparative Example 1 Fruit 10 ppm 45.2 Hydroquinone 1 ppm 41.1

From the results shown in Table 11, the ginseng root extract and fruit extract of Example 1 according to the present invention showed a better melanin production inhibitory rate than the ginseng extract and red ginseng extract of Comparative Example 1. Thus, it was confirmed that the roots and fruit extracts of the ginseng root of Example 1 according to the present invention showed excellent whitening effect.

2) Whitening effect on human skin

The following experiment was conducted to examine the whitening effect of the ginseng root and fruit extract of Example 1 against human skin.

First, 12 healthy boys were exposed to 1.5 cm diameter opaque tape at the upper abdomen of the subject. Ultraviolet rays (UVB) of about 1.5 to 2 times of the minimum erythema dose of each subject were observed. To induce blackening of the skin.

The ginseng roots and fruit extract of Example 1, which is the test substance after the ultraviolet ray irradiation, and hydroquinone were applied to each other, and one state was observed for 10 weeks without any application. The color of skin was measured with a colorimeter CR2002 (Minolta Co., Japan) every 1 week.

Then, the difference (ΔL *) between the start time of application of the respective test materials and the completion time of the application of the test materials was calculated according to the following equation (4). On the other hand, the whitening effect is judged by comparison of DELTA L * between the sample application region and the control region. When the DELTA L * value is 2, the whitening of the deposited pigment is noticeable. .

Test substance The degree of skin color brightness (DELTA L *) Example 1 Root 1.29 ± 0.18 Example 1 Fruit 1.78 ± 0.21 Hydroquinone (positive control) 1.90 + 0.13 Vehicle (negative control) 0.50 0.16

From the results of Table 12, it was confirmed that the roots and fruit extracts of the Ginseng root of Example 1 according to the present invention had a degree of skin color brightness similar to that of hydroquinone. This is because the Hwang sookjong ginseng roots and fruit extracts improve pigmentation caused by ultraviolet rays to brighten skin color.

[Test Example 4] Skin moisturizing effect and atopic symptom improvement effect

1) induction of differentiation of human keratinocytes

(CE) produced during differentiation of keratinocyte and human skin cell line (HaCaT) were measured to investigate the promoting effect of ginseng root and fruit extract of Example 1 according to the present invention on cell differentiation.

The primary cultured human keratinocytes were placed in a culture flask and adhered to the bottom. The test substances described in the following Table 13 were added to the culture medium by concentration, and the cells were cultured for 5 days until the cells reached 70-80% Lt; / RTI &gt; The cells were harvested, washed with PBS (phosphate buffered saline), and resuspended in 10 mM Tris-HCl buffer (Tris buffer, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) -HCl, pH 7.4), sonication, boiling, and centrifugation were resuspended in 1 ml of PBS, and the absorbance at 340 nm was measured. Separately, a part of the solution after the sonication was taken to measure the protein content, and was used as a standard for evaluation of the degree of cell differentiation. The test results are shown in Table 13 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups are used as negative / positive control groups and low calcium concentration test materials are added.

Test substance The ability to differentiate in keratinocytes (%) Control group The low calcium treatment group (Ca ^{2 +} 0.03 mM) 100 The calcium treatment group (Ca ^{2 +} 1.2 mM) 210 Red ginseng extract (100 ppm) 112 Example 1 Root 1 ppm 103 10 ppm 107 100 ppm 109 Example 1 Fruit 1 ppm 111 10 ppm 116 100 ppm 121

From the results shown in the above Table 13, it can be confirmed that the extract of Root and Ginseng Root of Example 1 according to the present invention promotes differentiation in keratinocytes.

2) Expression effect of transglutaminase in human skin cell line

Insert the 5 × 10 ⁴ dogs human skin cell lines in each well of a 96-well plate culture medium was attached for 24 hours. After the adhered skin cell line was treated with the test substance, after 2 days, the medium was removed and stored in a -20 ° C refrigerator. Cells treated with the substance were treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C for 30 minutes at 4 ° C, followed by freezing-thawing twice Cells were fixed. Then, the mixture was allowed to stand at room temperature to allow the organic solvent to evaporate, and blocking (1% bovine serum albumin), transglutaminase antibody (primary antibody) and HRP anti-mouse antibody (secondary antibody) (o-phennyldiamine). Absorbance was measured at 490 nm and calibration was performed by measuring the background at 630 nm. The test results are shown in Table 14 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative / positive control groups and low calcium concentration test substances were added.

Test substance Expression amount (%) of transglutaminase Control group The low calcium treatment group (Ca ^{2 +} 0.03 mM) 100 The calcium treatment group (Ca ^{2 +} 1.2 mM) 140 Red ginseng extract (100 ppm) 131 Example 1 Root 1 ppm 106 10 ppm 119 100 ppm 127 Example 1 Fruit 1 ppm 110 10 ppm 142 100 ppm 180

From the results of Table 14, it can be seen that the ginseng root extract and fruit extract of Example 1 of the present invention are expressed more than the control group. Therefore, it was confirmed that the roots and the fruit extract of the ginseng root of Example 1 according to the present invention promote the expression of transglutaminase.

3) Increased production of epidermal lipid (total ceramide) in human skin

In order to evaluate the effect of ginseng roots and fruit extract of Example 1 on the increase of lipid synthesis in human skin, 0.5% of Carbopol ETD 2020, 0.45% of TEA, 5% of test substance and 100 of deionized water Respectively. Twelve adult male and female subjects were divided into two groups of 50ml polypropylene cornical tube diameters on the forearm, and then the control group and 20mg of the extract of Ganoderma lucidum extract of Example 1 were applied to the subject Were divided into two groups, and the lipid was extracted and analyzed by the following method on the 3rd and 11th days. The forearms were washed with tap water and then tape stripped once with Scotch 810 Magic tape and cut into 50 ml polypropylene cornic tubes as a reservoir in 1 ml cyclone After the addition of cyclohexane / ethanol (4: 1), the mixture was mixed for about 1 minute, transferred to a new tube, treated with 1 ml of cyclohexane / ethanol (1: 1) . Each sample was dissolved in 500 μl of chloroform / methanol (2: 1) and dried on silica gel TLC (Thin Layer Chromatography) plate using Automated TLC sampler-4 from CAMAG And developed with developing solvent of composition ratio as shown in Table 15 using AMD (Automated Multiple Development).

chloroform Methanol water Acetic acid Hexane Diethyl ether Petroleum ether Solvent migration distance (cm) Primary 40 10 One - - - - 6.0 Secondary 190 9 - - - - - 6.5 Third - - - 2 12 3 - 7.5 Fourth - - - - - - 100 Top

The TLC plate was developed and the size of each band was measured at a wavelength of 570 nm using a TLC scanner-II (CAMAG: densitometer). The amount of ceramide produced in each treatment group in which the amount of ceramide produced in the non-treated group was converted to 100% is shown in Table 16 below.

Test substance After 3 days treatment (%) After 11 days treatment (%) Control group Untreated group 100 100 Vehicle 103 98 Glycerin (5%) 102 94 Example 1 Roots (5%) 104 108 Example 1 Fruit (5%) 107 114

The results of Table 16 show that the growth of Ginseng Root and Fruit Extract of Example 1 was increased after 3 days of treatment compared to the negative control group (untreated group / vehicle) and increased by 2% and 6% over the positive control group (glycerin) It looked. And 11 days later, lipid production was significantly superior to that of the negative control (carrier) and the positive control (glycerin). This demonstrates the increase of ceramide by roots and fruit extracts of the ginseng root of Example 1 according to the present invention.

4) Increase skin moisture in human body

40 male and female adult male and female adults exhibiting dry skin syndrome were divided into 4 groups. The nutritional creams of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 and 2 were applied twice daily to the face for 4 weeks. (24 ° C, relative humidity: 40%) after one week, two weeks, and four weeks after application and before two weeks (six weeks after completion of application) Skin moisture was measured and the results are shown in Table 17 below. The test result is a percentage of the increment of the measured value after a certain period of treatment based on the coneometer value measured just before the start of the test. The coneometer is a skin moisture meter for measuring the amount of water present in the skin by measuring the electrical conductivity of the skin.

Test substance Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Formulation Example 1 30 34 33 15 Formulation Example 2 34 42 42 32 Comparative Formulation Example 1 28 31 32 14 Comparative Formulation Example 2 32 40 41 29

In the results shown in Table 17, the skin moisture content was further increased in the group to which Formulation Examples 1 and 2 containing the extract of Root Ginseng Root and Fruit Extract of Example 1 were applied compared to the control group to which Comparative Formulations 1 and 2 were applied. In addition, the values of skin moisture measured 2 weeks after the application of the nutritional cream of Formulation Examples 1 and 2 were stopped (total of 6 weeks) were similar to those after 1 to 2 weeks, It was confirmed that the skin moisture was continuously maintained by applying the extract of Rootstock ginseng root and fruit extract according to the present invention for a certain period of time without further application.

5) Improvement of atopic dermatitis

The effect of improving atopic symptoms was measured as follows. Forty patients between the ages of 10 and 50 who were diagnosed with atopic symptoms (itching, eruption, severe dryness) or who had atopic symptoms by visual observation were divided into 4 groups and were classified into Formulation Examples 1, 2 and Comparative Formulation Examples 1,2 nutritional creams were applied to the area showing atopic symptoms twice a day for 12 weeks. The efficacy evaluation was conducted after 12 weeks' treatment, and the degree of improvement was judged subjective by the questionnaire. In the questionnaire, symptoms of atopic dermatitis were divided into "itching", "dryness", "keratinization", "dandruff", "erythema", "swelling", "skin crack" , The degree of improvement was evaluated for each symptom, and then the average was evaluated to evaluate the improvement effect of atopic symptoms. The results are shown in Table 18 below.

Test substance Number of respondents (in) Significant improvement Improving No change worse Formulation Example 1 3 6 11 0 Formulation Example 2 6 9 5 0 Comparative Formulation Example 1 2 4 14 0 Comparative Formulation Example 2 5 9 6 0

From the results shown in Table 18, it can be seen that the group to which Formulation Examples 1 and 2 containing the extract of Root-root ginseng and the fruit extract of Example 1 according to the present invention applied had generally atopic symptoms And further improvement was confirmed.

[Test Example 5] Acne and skin troubles were alleviated

1) sebum control effect: 5α-reductase inhibitory effect

Mature SD (Sproque-Dawley) male rats (7-8 weeks old) were sacrificed with diethyl ether, the abdominal prostate was removed, the connective tissue was removed, and a buffer (0.32 M sucrose, 0.1 mM dithiothreitol, And 20 mM sodium acetate), and then suspended in a pulverizer. The suspension was centrifuged and the supernatant was taken to partially refine 5α-reductase, and the activity of the enzyme was inhibited.

A portion of the supernatant obtained above is taken in a buffer containing 0.2 M base acid and 0.2 M dibasic acid, and then reacted with a substrate (testosterone) with an isotope, ³ H, to measure the amount of product dihydrootestosterone Respectively.

The reaction solution contained 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7- ³ H] testosterone / testosterone (2.2 × 10 ⁹ mol) Mg) &lt; / RTI &gt; protein.

The roots and fruit extracts of the ginseng root of Example 1 were dissolved in 10% ethanol and added to 10 μl of 100 μg / 10% ethanol per reaction. The same volume of solvent was used as a control, and riboflavin was used as a positive control.

The reaction was started at the same time as the addition of the enzyme suspension and proceeded at 37 DEG C for 30 minutes, followed by extraction with 1 mL of ethyl acetate. 100 mu l of the ethyl acetate phase was applied onto a silica plastic sheet kieselgel 60 F254), the developing solvent system was developed with acetate-cyclohexane (1: 1).

After the plastic sheet was air-dried, the bath system was used to measure the amount of isotope. The dried plastic sheet and x-ray film were put together in a bath cassette and after one week, the testosterone and dihydrootestosterone isotope And the results for each inhibition rate are shown in Table 19 below.

Test substance T (dpm) DHT (dpm) Conversion Rate (%) Inhibition rate (%) Example 1 Root 7122 3987 38.5 25.6 Example 1 Fruit 6878 2355 25.5 39.9 Control group 7520 5536 42.4 - Riboflavin 6300 2530 28.7 32.4 (1) T: 3H radioactivity in the testosterone area
(2) DHT: 3H radioactivity in the dihydrotestosterone region
(3) Conversion rate: Radio activity in the DHT area / Total radio activity
(4) Inhibition rate: 100 x (Conversion rate of control group - Conversion rate of sample) / Conversion rate of control group

From the results shown in Table 19, it was found that the extracts of Root and Ginseng Root and Fruit Extract of Example 1 had an inhibitory effect on 5α-reductase, and the extracts of Fruit ginseng had a better inhibitory effect than the positive control riboflavin.

2) Inhibitory effect on lipogenesis

The extent of lipid synthesis in the sebaceous glands was evaluated by assessing the extent of uptake of carbon elements required for lipid synthesis and assessing the degree of lipid synthesis inhibition.

In other words, the amount of inhibition was evaluated by biopsy of hamster ear tissues having a large number of sebaceous glands, and comparing and quantifying the test substance with the control group using a radioactivity measuring device for 6 hours on a C14 labeled medium. Test substances were used by adding 0.01% test substance solution to the medium using the right ear tissue of the hamster, 0.01% physiological saline was added to the medium using the hamster left ear tissue, and the results are shown in Table 20 .

On the other hand, the inhibition rate in Table 20 is calculated according to the following equation (5), and the value is an average value for four hamsters.

Test substance Inhibition rate (%) Example 1 Root 33 Example 1 Fruit 47 The positive control (retinoic acid) 52

From the results shown in the above Table 20, it was confirmed that the root extract and ginseng root extract of Example 1 of the present invention had lipid biosynthesis inhibitory effect.

3) Effect of Cotton Reduction

In order to confirm the effect of reducing cottonseed, the roots and fruit extracts of the ginseng root of Example 1 used in the present invention were dissolved in 1,3-butylene glycol to make a 1% solution and used as test materials for rabbit clinical experiments.

In the white rabbit ear, 0.2 ml of 50% oleic acid / paraffin oil, which is a skin inducing substance, was applied to the ear and rubbed with a cotton swab. Twice daily (50% oleic / paraffinic oil) and in the afternoon (substance) and applied for one month, the total duration of the experiment. The cotton seedlings occurred from about 10 days after seeding. The seedlings of the seedlings of Ginseng root and fruit extract of Example 1 were sprayed with 0.2 ml in the afternoon. As a result, the number and size of cotton seedlings gradually decreased after 2 weeks.

Each tissue was biopsied and the keratin was peeled off, and the size of the skin was measured under a microscope. The results are shown in Table 21 below.

Test substance Average skin size measurement (0.1 mm) N = 3 Control group (untreated group) 1.78 Oleic acid (OA) 3.5 Oleic acid (OA) + Example 1 root 2.9 Oleic acid (OA) + Example 1 Fruit 2.4

From the results of Table 21, it was confirmed that the rabbit skin treated with the extract of Root and Ginseng root of Example 1 of the present invention was significantly reduced. Namely, sebum reduction was observed by the roots and fruit extracts of the ginseng root of Example 1, and as a result, skin and keratinization were reduced, and it can be estimated that cottonseed decreased.

4) Effect of suppressing sebum secretion

In order to measure the effect of oily skin mitigation, 20 women aged 20 to 45 years, 20 men with oily skin, 10 men in the forehead region, were given Formulation Examples 1 and 2 (containing Hwang sookjong ginseng roots and fruit extract) 1 and 2 were applied twice a day in the morning and evening. After 1 week, 2 weeks, 3 weeks and 4 weeks, skin sebum secretion amount was measured using a sebum smear meter (Sebumeter SM810). The experiment was performed at 20 ° C and a relative humidity of 20%. The measurement results are shown in Table 22 below. The results were expressed as the average value of 10 subjects. In the case of oily skin, the measurement value is 220 or more, and in the case of normal skin, it is between 100 and 220.

Test substance Sebum secretion amount (A.U) Before application 1 week 2 weeks 3 weeks 4 weeks Formulation Example 1 245 240 239 235 230 Formulation Example 2 245 236 232 221 216 Comparative Formulation Example 1 245 238 237 228 224 Comparative Formulation Example 2 245 236 231 218 209

From the results of Table 22, it can be seen that the nutritional cream of Formulation Examples 1 and 2 containing the root extract and ginseng root extract of Example 1 according to the present invention has an effect of suppressing excessive sebum secretion, there was.

5) Anti-inflammatory effect: Inhibitory effect of prostaglandin E2 (PGE2) production

In order to investigate the inhibitory effect of the ginseng roots and the fruit extract of the present invention on the production of prostaglandin E2, the following experiment was conducted (Reference: Jeffrey, KH, Anglea, SW, Zoe, S., and Rhia CM Intracellular Measurement Prostaglandin E2: Effect of Anti-inflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28).

First, fibroblasts isolated from human epidermal tissue were cultured in a CO ₂ incubator for 24 hours, and then cultured for 2 hours in a medium containing no fetal bovine serum (FBS). Then, The roots and fruit extracts were mixed with distilled water at concentrations of 1, 10 and 100 ㎍ / mL, respectively, and then cultured in a CO ₂ incubator at 37 ° C and 5% (v / v) for 2 hours.

Then, calcium iodate A23187 (Calcium ionophore A23187) and arachidonic acid were treated for 50 minutes as a prostaglandin E2 stimulating agent for 5 minutes, the cells were hemolyzed, and the absorbance was measured at 450 nm in an ELISA reader to quantitate prostaglandin E2 And the results are shown in Table 23 below. At this time, the inhibition rates shown in Table 23 below are shown in terms of the amount of prostaglandin E2 produced in fibroblasts treated with arachidonic acid and the skin fibroblasts not treated with the ginseng root and fruit extract of Example 1 and fibroblasts treated with the ginseng root and fruit extract of Example 1 , And the mean value was obtained by repeating 5 times.

Test substance density
(쨉 g / mL) Prostaglandin E2 (pg / 10 ⁵ cells) % Inhibition Negative control group - 25.2 ± 4.4 - Stimulus circle - 395.1 + - 9.1 - Stimulation circle + Example 1 root One 311.2 ± 6.8 21.2 10 266.7 ± 7.6 32.5 100 238.6 ± 14.3 39.6 Stimulation circle + Example 1 Fruit One 286.4 ± 6.5 27.5 10 249.2 ± 8.6 36.9 100 198.6 ± 14.3 49.7

From the results shown in the above Table 23, it can be confirmed that the ginseng root extract and fruit extract of Example 1 of the present invention can inhibit the production of prostaglandin E2 by the stimulus source.

6) Anti-inflammatory effect: inhibition of biosynthesis of cyclooxygenase-2 (COX-2) induced by LPS (lipopolysaccharide)

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then treated with LPS. Then, the medium was replaced with media containing 1, 10, and 100 ppm of the Hwang sookjong ginseng root and the fruit extract of Example 1. On the second day of culture, the cells were harvested and the amount of cyclooxygenase-2 (COX-2) produced using the Westren blot method was quantitated. The comparison with the value measured with a densitometer with the control group as 100 is shown in Table 24 below. Here, the control group was a non-treated group in which the roots of Ginseng root and the fruit extract of Example 1 were cultured without treatment.

Test substance COX-2 biosynthesis (%) Example 1 Root 100 ppm 11 10 ppm 30 1 ppm 59 Example 1 Fruit 100 ppm 14 10 ppm 36 1 ppm 70 Control group 100

From the results of Table 24, it can be seen that the roots and fruit extracts of the ginseng root of Example 1 according to the present invention inhibit the inflammatory reaction by reducing the biosynthesis of cyclooxygenase-2 induced by LPS, which is an inflammatory reaction factor, It was confirmed that the skin trouble can be alleviated.

7) Anti-inflammatory effect: Inhibition effect of LPS (Lipopolysaccharide) -induced tumor necrosis factor (TNF-α)

Human keratinocytes were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and were treated with LPS and then replaced with media containing 1, 10, and 100 ppm of Hwang sookjong ginseng roots and fruit extracts of Example 1. After culturing for 6 to 24 hours, the cells were harvested and the amount of TNF-a produced was quantitated using ELISA (Pharmingen 555212) method. Table 25 shows the comparison with the value measured with the control group as 100. [ Here, the control group was a non-treated group in which the roots of Ginseng root and the fruit extract of Example 1 were cultured without treatment.

Test substance TNF-α biosynthesis (%) Example 1 Root 100 ppm 10 10 ppm 32 1 ppm 55 Example 1 Fruit 100 ppm 13 10 ppm 40 1 ppm 68 Control group 100

From the results of Table 25, it can be seen that the roots and fruit extracts of the ginseng root of Example 1 according to the present invention reduce the biosynthesis of TNF-? By inflammation inducer LPS in a concentration-dependent manner, And contributed to improvement of trouble.

8) NO inhibition effect induced by LPS on macrophages

In order to examine the antiinflammatory effect of the root extract and the ginseng root extract of the present invention, LPS-induced NO (nitric oxide) inhibition experiment was performed on macrophages.

Macrophages (Raw 264.7 cells) were cultured in 5% CO ₂ medium supplemented with 10% serum. The cells were grown in a 96-well plate incubator at a concentration of 2 × 10 ⁵ , stimulated with LPS (1 ug / ml), treated with the ginseng roots and fruit extract of Example 1 at 37 ° C. for 1 hour, The results are shown in Table 26. The results are shown in Table 26 below. The NO production was measured by the Griess reaction method (Minghetti, L. et al., 1991, Glia 19. 152-160).

Test substance NO production (μM) Example 1 Root 100 ppm 8.9 ± 0.2 50 ppm 10.1 ± 0.3 25 ppm 13.8 ± 0.5 Example 1 Fruit 100 ppm 7.6 ± 0.2 50 ppm 9.7 ± 0.2 25 ppm 11.2 ± 0.3 LPS induction 19.1 ± 0.5 Control group 1.2 ± 0.1

From the results shown in Table 26, it was found that the extract of Root and Panax ginseng of Example 1 according to the present invention effectively inhibited NO production as an inflammatory factor (Cytokine). Therefore, it is expected that the roots and fruit extracts of the ginseng root of Example 1 according to the present invention contribute to the effect of healing wounds caused by skin troubles and suppressing wrinkle formation by controlling the inflammatory reaction.

9) Acne and skin trouble improvement effect

The participants were 20 women who had severe skin troubles including acne, and their face was soaked in soap for 30 minutes in the temperature and humidity room, and the degree of skin trouble was measured by comparing the number of acne and inflammation .

The subjects were allowed to use the nutritional creams of Formulation Examples 1, 2 and Comparative Formulation Examples 1 and 2 for 4 weeks, respectively, and then the degree of acne and skin troubles were compared and the results are shown in Table 27 below.

Evaluation items Formulation Example 1 (5 persons) Formulation Example 2 (5 persons) Comparative Formulation Example 1 (5 persons) Comparative Formulation Example 2 (5 subjects) Skin Trouble Improvement Ratio 60% 80% 20% 60%

As can be seen from the results of Table 27, when Formulation Examples 1 and 2 containing the extract of Rootstock ginseng and fruit extract of Example 1 according to the present invention were applied for 4 weeks, reduction in the number of existing acne and improvement of inflammatory accompanying trouble .

[Formulation Example 3] Softening lotion (skin lotion)

The softening longevity was prepared according to a conventional method according to the composition shown in the following Table 28 (unit: wt%).

Compounding ingredient content Purified water Balance The ginseng extract of Example 1 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservatives, coloring and flavoring Suitable amount

[Formulation Example 4] Nutritional lotion (milk lotion)

Nutrition lotion was prepared in a conventional manner according to the composition shown in the following Table 29 (unit: wt%).

Compounding ingredient content Purified water Balance The ginseng extract of Example 1 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, coloring and flavoring Suitable amount

[Formulation Example 5] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 30 below (unit: wt%).

Compounding ingredient content Purified water Balance The ginseng extract of Example 1 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, coloring and flavoring Suitable amount

[Formulation Example 6] Pack

A pack was prepared in a conventional manner according to the composition shown in Table 31 below (unit: wt%).

Compounding ingredient content Purified water Balance The ginseng extract of Example 1 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Phage-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservatives, coloring and flavoring Suitable amount

Claims (9)

A cosmetic composition for improving skin wrinkles containing an extract of ginseng as an active ingredient. A cosmetic composition for improving skin elasticity containing an extract of ginseng as an active ingredient. Cosmetic composition for moisturizing skin containing ginseng extract as an active ingredient. A cosmetic composition for improving atopic symptoms containing an extract of ginseng as an active ingredient. A cosmetic composition for controlling sebum containing an extract of ginseng as an active ingredient. (EN) Cosmetic composition for anti - inflammation containing ginseng extract as an active ingredient. 7. The cosmetic composition according to any one of claims 1 to 6, wherein the composition contains 0.001 to 50% by weight of a yellow ginseng extract based on the total weight of the composition. The cosmetic composition according to any one of claims 1 to 6, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, A powder, a body lotion, a body cream, a body oil, and a body essence. [Claim 7] The cosmetic composition according to any one of claims 1 to 6, wherein the ginseng extract is an extract of roots, or fruits, or roots and fruits of the ginseng root.