KR20110078360A - Composition containing ginseng berry extract for hair growth - Google Patents

Abstract

PURPOSE: A composition containing ginseng berry extract which relieves scalp irritation and inflammation is provided to ensure less skin irritation. CONSTITUTION: A composition for promoting hair growth contains 0.001-30 weight% of ginseng berry extract as an active ingredient. The composition is a cosmetic composition manufactured in the form of hair tonic, hair lotion, hair rinse, shampoo, and hair treatment. A method for preparing the extract comprises: a step of removing seed from ginseng berries and drying ginseng berries by the sun light or hot wind; a step of adding 3L of water to 1kg of dried ginseng berries and refluxing; and a step of filtering and compressing at 40-45 degrees Celsius.

Description

Composition for promoting hair growth containing ginseng fruit extract {Composition containing ginseng berry extract for hair growth}

The present invention relates to a composition for promoting hair growth containing a ginseng fruit extract, and more specifically, by containing a ginseng fruit extract having a unique ingredient and composition as an active ingredient in the ground portion of ginseng, it may appear during chemical procedures such as dyeing and perming. The present invention relates to a composition for promoting hair growth, which provides an effect of relieving irritation and inflammation such as itching and stinging of a scalp and promoting hair growth.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi ginseng and has been used in Korea, China, and Japan for more than 2,000 years, and has been used to prevent disease and extend life. Known efficacy and effects of ginseng to the central nervous system, anticarcinogenic effect, anticancer activity, immune function regulation, antidiabetic effect, liver function anti-inflammatory effect, cardiovascular disorders improvement, anti-arteriosclerosis, blood pressure control action, menopausal Disability improvement, effects on osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effects (Korea Ginseng & Tobacco Research Institute, 56-112, 1996).

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly in the ground and underground parts of ginseng. Especially, the composition and composition of ginsenosides are different depending on the parts such as ginseng root (root), ginseng leaf and ginseng fruit. (Attele AS et al, Biochem Pharmacol, 58; 1685-1693, 1999).

On the other hand, the hair of the human body is about 100,000 to 150,000, each hair will grow and degenerate through different cycles. Hair is a growing period (Anagen), a degenerative period (Catagen) where metabolic processes slow down while maintaining the shape of the hair, and the hair papilla is contracted and the hair follicles are contracted gradually so that the hair roots are pushed upwards and the hair follicles become smaller (Telogen) The cycle and lifespan of the hair can be changed according to various conditions such as nutrition status, medical history, heredity, constitution, hormone secretion or aging.

Hair loss is a problem that occurs through natural processes or is chemically promoted by the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss involves the lack of hair regrowth that causes partial or complete baldness.

Recently, as more and more modern people have alopecia due to environmental pollution, stress, diet, fatigue, and incorrect eating habits, more and more men or women are suffering from severe mental pain.

Therefore, the present inventors have no side effects on the scalp and have a stimulating effect on use of all the strengthening and scalp, and at the same time, as a result of studying a composition that can provide a hair loss prevention and hair growth effect, ginseng fruit extract is 5 alpha-reducing enzyme The present invention was found to be able to inhibit the activity of and provide an effect of promoting hair growth.

Accordingly, it is an object of the present invention to provide a composition for promoting hair growth with low skin irritation and excellent use.

In order to achieve the above object, the present invention provides a composition for promoting hair growth containing ginseng fruit extract as an active ingredient.

The composition of the present invention has the effect of relieving scalp irritation and inflammation by containing ginseng fruit extract as an active ingredient, inhibits the activity of 5 alpha-reducing enzymes and strengthens the hair root, thereby reducing hair loss and promoting hair growth. .

As used herein, the term "promoting hair growth" means not only promoting the production of new hair, but also ensuring that existing hair grows healthy, and prevents the phenomenon of hair falling off from the scalp and the condition of the hair becoming thin or thin. And restraint.

The composition for promoting hair growth according to the present invention contains ginseng fruit extract as an active ingredient, and the ginseng fruit extract has an effect of inhibiting the activity of 5 alpha-reductase which promotes the production and activity of dihydrotestosterone that induces hair loss. There is.

5 alpha-reductase is present in male hormone-reactive tissues such as sebaceous glands, hair follicles, prostate or epididymis, and testosterone, a male hormone that is biosynthesized in the testes, is an active male hormone, dihydrotestosterone (DHT). It is known to be a reductase involved in the conversion of NADPH as a coenzyme. Excessive secretion of dihydrotestosterone after puberty acts as a major causative agent in the production of sebum in sebocytes, causing acne and male hair loss. In particular, many hair follicle cells provide a cause for hair loss because dihydrotestosterone binds to hormone receptors better than testosterone.

Therefore, the composition for promoting hair growth according to the present invention has a 5 alpha-reductase inhibitory effect by using ginseng fruit extract as an active ingredient, thereby promoting hair growth and preventing hair loss. In addition, scalp stimulation may be alleviated, such as reduction of irritation such as itching and stinging of the scalp and scalp inflammation, and the scalp stimulation includes stimulation that may appear during chemical treatment such as staining and perming. In addition, the composition of the present invention can prevent hair thinning due to scalp damage by strengthening the hair root.

The ginseng fruit extract according to the present invention is prepared by extracting the flesh and skin of the ginseng fruit separated and separated from the seeds by sun drying or hot air drying, followed by extraction with water or ethanol.

The composition of the present invention preferably contains the ginseng fruit extract in an amount of 0.001 to 30% by weight based on the total weight of the composition. This is because when the content of the active ingredient is less than 0.001% by weight, the efficacy and effect are weak, and when it exceeds 30% by weight, there is a problem in stabilizing the formulation.

Hair growth promoting composition of the present invention is not particularly limited in formulating, but is a cosmetic composition having a formulation such as hair tonic, hair nourishing longevity, scalp treatment, hair treatment, hair rinse, hair shampoo, hair lotion, etc. Can be.

In addition, in each formulation, other ingredients in addition to the above-mentioned essential ingredients may be appropriately selected and blended by those skilled in the art without difficulty according to the kind or purpose of use of other external preparations.

Hereinafter, although an Example and a test example are given and demonstrated more concretely of this invention, this invention is not limited only to these examples.

Example 1 Preparation of Ginseng Fruit Extract

1. Ginseng Fruit Pretreatment

Raw ginseng fruit was harvested, seed was separated and removed, and then the ginseng fruit dried raw material was prepared by sun drying or hot air drying.

2. Ginseng Fruit Extract Manufacturer

3L of water was added to 1 kg of dried ginseng fruit, and the mixture was extracted under reflux, filtered and concentrated under reduced pressure at 40 to 45 ° C. to obtain 300 g of ginseng fruit extract.

Comparative Example 1 Preparation of Ginseng Root Extract

Except for using ginseng root (root) instead of ginseng fruit in Example 1 was prepared in the same manner as in Example 1.

Reference Example 1 Comparison of Ingredients of Ginseng Fruit Extract

<Analysis of Ginsenoid (Ginseng Saponin) in Ginseng Fruit and Ginseng Root>

In Example 1 and Comparative Example 1, respectively, ginseng fruit and ginseng root extract were prepared, and then, the extracts were treated with ether to remove fat-soluble components, followed by extracting and concentrating ginsponin with butanol (BuOH). Cenoside component analysis was performed and the results are shown in FIG. 1 and Table 1. FIG.

Example 1 Comparative Example 1 Irradiation Ponine Content
(Dry weight) 33.42% 16.70% PD / PT ratio 0.73 3.23

The ginseng fruit extract prepared in Example 1 had about twice the content of the ginseng root extract prepared in Comparative Example 1 in the content of irradiated phonynin, and ginsenosides were PD (Protopanaxadiol)-"ginsenoside Rb1, Rb2. And Rc and Rd "and PT (Protopanaxatriol)-" Ginsenoside Re, Rg1 and Rg2 "ratios were 0.73 and 3.23, respectively. .

Mineral Analysis of Ginseng Fruit Extract

In order to distinguish the ginseng fruit extract prepared in Example 1 has the characteristics as 'fruit' different from ginseng, mineral components including vitamins were analyzed, and the results are shown in Table 2 below.

ingredient content ingredient content Potassium (mg / 100g) 5865.57 Magnesium (mg / 100g) 354.38 Calcium (mg / 100g) 819.26 Zinc (mg / 100g) 178.49 Iron (mg / 100g) 59.31 Vitamin A (µg / 100g, RE) 213.11 Phosphorus (mg / 100g) 187.17 Vitamin B1 (mg / 100g) 12.29 Vitamin B2 (mg / 100g) 8.45 Vitamin B6 (mg / 100g)  10.50 Vitamin C (mg / 100g)  4.91 Vitamin E (mg / 100g, a-TE) 23.61 Vitamin K (μg / 100g) 232.12 Niacin (mg / 100g, NE) 5.76 Pantothenic Acid (mg / 100g) 5.87 Folic Acid (µg / 100g)  349.97

As described above, the ginseng fruit used in the present invention contains more ginseng saponin than the ginseng root, and at the same time, the composition of the saponin is opposite to that of the ginseng root. It was confirmed that, based on this was carried out the following experiments related to hair growth promotion.

[Test Example 1] 5 alpha-reductase inhibitory effect test using Sprague Dawley Prostate

7-8 weeks old male Sprague-Dawley was killed with diethylether and the abdominal prostate was removed to remove connective tissue. Buffer (0.32M sucrose, 0.1 mM dithiothreitol, 20 mM sodium acetate) was added thereto, chopped finely by a grinder and suspended. The suspension was filtered with gauze, and the filtrate was centrifuged at 1500 × g, pellets were taken, and the buffer solution was mixed and suspended at a concentration of 20-30 mg / ml.

After taking a portion of the suspension was placed in a 0.02M phosphate buffer, it is reacted with a substrate which is a testosterone isotopes ³ H attached to measure the amount of dihydrotestosterone product. At this time, the reaction solution is dithiothreitol, 20mM of phosphate buffer (pH 6.5) in 1mM, of 50μM NADPH, [1,2,6,7- ³ H] testosterone 0.1μCi / testosterone (1 × 10 ^-7 M ) And 36 μg enzyme suspension were added to prepare a total reaction solution of 565 μl.

A certain amount of the ginseng fruit extract of Example 1 and the ginseng root extract of Comparative Example 1 were dissolved in 10% dimethyl sulfoxide (DMSO) and added to 10 μl per reaction, and the same volume of solvent was used as a positive control. Linolenic acid was used.

In addition, the reaction was carried out at 37 ° C. for 30 minutes by adding the prepared enzyme suspension. Then, 600 µl of ethyl acetate was added to stop the reaction, and the product was dried through nitrogen gas. This is a drip on the total amount of ethyl acetate to the silica plastic sheet kayijel gel 60 F was dissolved in ₂₅₄ of 50㎕ (Silica plastic sheet kieselgel 60 F ₂₅₄₎ and then a developing solvent ethyl acetate: (1: 1) cyclohexane (cyclohexane) Developed using.

After drying the silica plastic sheet in the natural state, a bath (Bas, FUJIFILM, Japan) system was used to measure the amount of isotopes, and the dried plastic sheet and the exposed film were put together in a bath cassette and stored for one week. The isotope amounts of testosterone and resulting dihydrotestosterone exposed to the film were then quantified using the exposed intensity.

The experiment was used repeatedly for two times, and based on the result of the measurement, each of 5 alpha-reductase inhibition rates was calculated by the following calculation method. The experimental results are shown in Table 3 below.

Item density Hormone density Conversion rate Average conversion rate Inhibition rate Control group - Testosterone 1.9 26.36 25.27 0.00 DHT 0.68 Positive control
(Linolenic acid) 10 ppm Testosterone 1.61 12.97 - 48.67 DHT 0.24 Example 1
(Ginseng Fruit Extract) 10 ppm Testosterone 2.58 1.90 - 92.48 DHT 0.25 Comparative Example 1
(Ginseng Root Extract) 10 ppm Testosterone 1.66 9.28 - 63.27 DHT 0.17 * Density: ³ H radioactivity in testosterone and dihydrotestosterone domains
* Conversion rate: Radiation amount / total radiation amount in DHT area
* Inhibition rate: 100 × (control rate-sample conversion rate) / control rate

As can be seen from the results of Table 3, although both ginseng fruit extract and ginseng root extract has a 5 alpha-reductase inhibitory effect, the 5 alpha-reductase inhibitory effect of the ginseng fruit extract used in the present invention is significantly high. You can see that.

[Test Example 2] 5 alpha-reductase inhibitory effect test using the fuzzy rat (Fuzzy rat)

Fuzzy rat males sold by Charles & River Lab, USA, are naturally controlled by male hormones and are sebum as testosterone is converted to dehydrotestosterone as described above. ) is generated has a characteristic that varies over the surface yellowing (Skin Pharmacol . 1997; 10 (5-6): 288-297). Using this purgelet, 5 alpha-reductase inhibitory effect of ginseng fruit extract was confirmed.

When a finasteride (Merck, USA) drug known to have an activity that inhibits 5 alpha-reductase, which is involved in testosterone metabolism, was administered to the purge red, it was confirmed that the surface of the rat did not turn yellow. The ginseng fruit extract of 1 was diluted to a concentration of 1.0% in a propylene glycol: anhydrous ethanol (7: 3) solution and applied twice a day for 6 weeks. At this time, using a propylene glycol: anhydrous ethanol (7: 3) solution containing no ginseng fruit extract as a control was applied for 6 weeks in the same manner. The observation result is shown in FIG.

1, the surface color of the fuzzy rat coated with the solution containing the ginseng fruit extract did not change (A), but the surface color of the fuzzy rat coated with only the propylene glycol: anhydrous ethanol (7: 3) solution changed yellow. B) You can check

Therefore, it can be seen from the above results that the extract of the present invention white porcelain has 5 alpha-reductase inhibitory effects.

Formulation Example 1 and Comparative Formulation Example 1 Preparation of Composition

To prepare a composition of Formulation Example 1 and Comparative Formulation Example 1 to the composition of Table 4.

Ingredient (% by weight) Formulation Example 1 Comparative Formulation Example 1 Example 1
(Ginseng Fruit Extract) 0.5 - Comparative Example 1
(Ginseng Root Extract) - 0.5 ethanol 50 50 Purified water balance
(to 100) balance
(to 100)

Test Example 3 Stimulation Relief Efficacy Test

In order to evaluate the efficacy of stimulating relaxation treatment by stimulation of hair dye, MEST method using mouse was applied. The test method is as follows.

Six-week-old BALB / c mice were purchased and constructed with a 10-day quarantine and acclimatization period. The test group was configured as follows. Hair was removed from the abdomen of the rat, and 2.5% paraphenylenediamine (PPD) was applied for 3-5 days. After a week of rest, hair dye was applied to the rats' ears and wiped off after 30 minutes. After a week of rest again, the compositions of Formulation Example 1 and Comparative Formulation Example 1 were applied twice at an hourly interval. 24 hours after the application of the hair dye, the compositions of Examples and Comparative Examples were applied twice. After 24 hours, the weight of the ear was measured, and the weight of the pathologic ear was measured by H & E staining. The hair dye formulation is shown in Table 5 below, and the measurement results are shown in Table 6 below.

ingredient Content (% by weight) Ingredient 1 Purified water To 100 Sodium sulfite 0.5 Asvo Kick Acid 0.5 Polyoxyethylene uryl ether 4.0 Polyoxyethylene oleyl ether 4.0 Cetostearyl alcohol 8.0 p-phenylenediamine 0.5 Resorcinol 0.5 Monoethanolamine Quantity ammonia Quantity 2nd ingredient Purified water 0.1 Hydrogen peroxide (30%) 17.0 Phosphoric Acid 0.1

Treated group Ear weight (mg) Ear thickness (㎛) Formulation Example 1 9.76 ± 0.945 268.11 ± 26.142 Comparative Formulation Example 1 14.45 ± 0.243 384.65 ± 22.988

As can be seen in Table 6, when the pre-treatment with the composition of Formulation Example 1 according to the present invention can be confirmed that the ear weight and the thickness of the ear is significantly reduced to reduce the inflammation. Thereby, it can be judged that the composition by this invention has the effect of alleviating the inflammation by a hair dye.

Test Example 4 In vivo Itching Inhibition Test

The anti-itch effect of the composition according to the present invention was tested. Hairless mice were used to facilitate skin application of the compositions of Formulation Example 1 and Comparative Formulation Example 1. The hairless mice were all 7-week-old males, and all groups used 8 hairless mice, respectively, for comparison with the negative control group.

Treatment of the external preparation was carried out by taking 150 μl of the compositions of Formulation Example 1 and Comparative Formulation Example 1 and spreading them on the back of hairless mice. The treatment was performed twice a day for 1 day divided into the morning and afternoon, and last 30 days before the injection of itching agent in the morning on the second day.

The itch test allowed 30 minutes to acclimatize a hairless mouse into an observation clear cage 30 minutes before the itch-injecting material was injected. At the end of the acclimatization time, compound 48/80 (compound 48/80), an itch-causing substance, was dissolved in physiological saline and intradermal injection was applied to the back skin of the adapted hairless mice. . Immediately after the injection, the hairless mice were placed in the cage for observation, and video was taken for 30 minutes. The number of scratches on the site where the itch-inducing substance was injected into the hind legs was measured and displayed in numbers. Except for scratching with the forefoot or biting by mouth, only the action of scratching the injection site with the hind feet was considered as an indication of the itch behavior, and the measurement results are shown in FIG. 2 and Table 7 below.

Treated group Itching Agent Average number of scratches in 30 minutes Negative control group Saline solution 16.7 ± 27.9 Formulation Example 1 C48 / 80 122.6 ± 25.8 Comparative Formulation Example 1 C48 / 80 140.5 ± 20.3

As can be seen in Figure 2 and Table 7, when the pretreatment with the composition of Formulation Example 1 according to the invention it can be seen that the number of scratches to reduce the effect of suppressing itching.

Therefore, the results of the above test for alleviating inflammation and suppressing skin itch symptoms, it can be seen that the composition according to the present invention can improve the scalp condition.

Test Example 5 Hair Loss Prevention Effect Test

Twenty subjects with initial hair loss were tested for anti-hair loss for 12 weeks. After the compositions of Formulation Example 1 and Comparative Formulation Example 1 were used once a day for 12 weeks, after 12 weeks, the hair loss was collected and counted again when the hair was washed, and the results are shown in FIG. 3.

As can be seen in Figure 3, when treated with the composition of Formulation Example 1 according to the present invention was confirmed that the number of the falling off hair significantly reduced compared to the case of the composition of Comparative Example 1.

Test Example 6 Hair Strength Test

Experiments were conducted on the hair strengthening effect of the compositions according to the invention.

Experimental tress hair was treated with the same amount of the composition of Formulation Example 1 and Comparative Formulation Example 1 and dried at room temperature for 30 minutes, and hair with a thickness of 80-90 μm was collected from the dried experimental tress hair. The experimental tress hair was cut 10 cm, and a paper weight of a certain weight was hung on both ends, and the hook was placed on a ring 7 cm above the bottom to balance both sides, and the distance between both ends of the experimental tress hair was measured with a vernier caliper. After experimenting with 15 hairs, the average distance was calculated, and the results are shown in Table 8 below.

Treated group Hair Distance (cm) Formulation Example 1 2.5 Comparative Formulation Example 1 2.1

As can be seen in Table 8, it can be seen that when treated with the composition of Formulation Example 1 according to the present invention, the hair distance is increased, which causes the effect of causing power to the hair.

Test Example 7 Hair Growth Effect Test

The hair growth effects of the compositions according to the invention were tested.

7 week old mice (C57BL / 6) were removed from the dorsal area of hair, and the back skin was selected to be clean, and seven animals were selected for each substance group. 150 μl per coat was applied for 21 days. As a negative control group, the peony extract was 0.5% by weight and dissolved in a solvent composed of water / ethanol / 1,3-butylene glycol (5/3/2).

The weight of the newly grown hair was measured and compared with the negative control group, and the results are shown in Table 9 below.

Treated group Hair weight (mg) Negative Control 26 ± 15 Comparative Formulation Example 1 60 ± 9 Formulation Example 1 75 ± 13

As can be seen in Table 9, when the Formulation Example 1 according to the present invention was applied, the average value of hair weight was higher than that of the group in which the general solution (water / ethanol / 1,3-butylene glycol) was applied. It was confirmed that there is an excellent hair growth promoting effect by promoting the transition to the growth hair cycle by acting on the mouse of the hair cycle.

Therefore, when the hair root is strengthened, the hair is generated, hair loss is prevented, and the hair growth effect is obtained. From the test results, it can be seen that the composition according to the present invention has the effect of strengthening the hair root. In addition, it can be confirmed that there is an excellent hair growth promoting effect and excellent hair growth promoting effect.

Figure 1 shows the results of testing the 5 alpha-reductase inhibitory effect of the composition according to the invention using a Fuzzy rat (Fuzzy rat).

2 is a graph showing the anti-itch effect of the composition according to the present invention.

Figure 3 is a graph showing the hair loss prevention effect of the composition according to the present invention.

Claims (3)

A composition for promoting hair growth containing ginseng fruit extract as an active ingredient. According to claim 1, wherein the ginseng fruit extract is a composition for promoting hair growth, characterized in that contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. The composition for promoting hair growth according to claim 1, wherein the composition is for inhibiting 5 alpha-reductase enzyme.

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