KR20110064338A - Cosmetic composition containing extract of ligustrum japonicum and hemerocallis fulva for improving skin wrinkle - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Hemerocallis fulva and Ligustrum japonicum extract is provided to improve antiwrinkling effect and to suppress elastase. CONSTITUTION: A cosmetic composition for anti-wrinkling contains 0.005-50 weight% of Hemerocallis fulva and Ligustrum japonicum extract in a weight ratio of 1:10-10:1 as an active ingredient. The composition is used in the form of skin lotion, skin toner, astringent, lotion, massage cream, hand cream, or eye shadow.

Description

Cosmetic composition for improving wrinkles containing extracts of mineral trees and cones as active ingredients {COSMETIC COMPOSITION CONTAINING EXTRACT OF LIGUSTRUM JAPONICUM AND HEMEROCALLIS FULVA FOR IMPROVING SKIN WRINKLE}

The present invention relates to a cosmetic composition for improving wrinkles containing mineral extracts and extracts, specifically, the effect of promoting collagen production, inhibiting the production of MMP-1 production and elastase inhibitory activity by containing a mixture of extracts and extracts as an active ingredient It relates to a cosmetic composition for wrinkle improvement excellent in effect.

The function of cosmetics is not only clean and aesthetic, but also free radicals and free radicals caused by external factors such as moisture, pollutants and stress, ultraviolet rays, and internal factors such as deterioration of immune cells and deterioration of cell activity. It has been extended to remove or suppress the cause of skin aging such as protein, nucleic acid, and cell membrane lipid destruction by peroxides. Among them, cosmetics for wrinkle improvement and suppression have received much attention from consumers who want to maintain youth.

Wrinkles are formed by repetitive movements of muscles in a specific direction over long periods of time, depending on age, external environment, and UV radiation. Damage to skin cells caused by these factors causes various aging phenomena. Collagen and elastin, which constitute the dermal matrix, are not easily produced and degraded.

Meanwhile, elastase is an enzyme that degrades elastin, an insoluble elastic fiber protein of animal connective tissue, and hydrolyzes all proteins including proteins that support and form connective tissues such as collagen and elastin. Elastase proteases have been identified in platelets, mononuclear leukocytes, macrophages, and smooth muscle cells, in addition to multinuclear leukocytes and pancreas. Leukocyte elastase belongs to the serine proteinase. That is, the nucleophilic group where the serine residue at position 195 in the primary sequence of the enzyme attacks the a-carbonyl of the valine or alanine of the substrate. Acts as. Fibroblast elastases, on the other hand, belong to metalloproteinases that contain metal ions in their active sites. Some of the elastase activity of multinucleated leukocytes may be useful in vivo, such as complement and lymphocyte activation, but excessive elastase activity is detrimental to the skin.

The mechanism of photoaging is known to decrease collagen synthesis by UV irradiation and increase the expression of matrix metalloproteinase, which leads to increased degradation of collagen and elastin, which are the major constituents of the substrate, and thus photoaging including wrinkles. have. Elastase is also an enzyme that decomposes elastic substances during the decomposition of elastic fibers, and its activity is increased by ultraviolet irradiation, which is involved in photoaging.

Recently, many studies have been published to improve skin wrinkles using natural materials. Korean Patent Publication No. 2008-1031 discloses a cosmetic composition for improving wrinkles containing Prunella vulgaris extract, and Korean Patent Publication No. 2008-94458 discloses Astragalus, Eoseongcho, Ginkgo, Euiin, Skirt Mushroom, Yellow white, loquat, aloe, Yulpi, birch, rose, kidney bean, zelkova, jacaranda, zinnia, betel nut, cephalopod, viburnum, jade porridge, light bulb, pilgrim, weathered flower, castor, reddish brown, tiger, hodo, pumpkin, hoi A cosmetic composition for improving wrinkles comprising fermented extract of at least one selected from the group consisting of vinegar, safflower, yellow flower and persimmon leaf as an active ingredient is disclosed, and Korean Patent Publication No. 2008-101821 discloses safflower extract as an active ingredient. It is disclosed with respect to the cosmetic composition for wrinkle improvement containing.

Therefore, the present inventors selected the herbal medicines effective in improving wrinkles, and as a result, the mixed extract of the light wood and the cone having a specific compounding ratio exerts a synergistic effect, suppresses the expression of elastase, and promotes the biosynthesis of collagen to reduce wrinkles. It was confirmed that the improvement and completed the present invention.

It is an object of the present invention to provide a cosmetic composition containing a mixture of extracts of light wood and cones to improve skin wrinkles. In particular, it is an object of the present invention to provide a cosmetic composition that improves wrinkles by inhibiting the activity of elastase, inhibiting MMP-1 production and promoting biosynthesis of collagen.

According to the present invention in order to achieve the above object, there is provided a cosmetic composition for improving wrinkles containing a mixture of light wood and cone. The mixed extract is a mixture of light wood extract and cone extract in a weight ratio of 1:10 to 10: 1, preferably the light wood extract and conical extract is mixed in a weight ratio of 1: 1.

The mixture of the light wood and the cone is contained in an amount of 0.005 to 50% by weight, preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition.

The mixed extract improves wrinkles by promoting collagen production in the skin. In addition, the mixed extract has a feature of improving skin wrinkles by inhibiting MMP-1 production in the skin and inhibiting the activity of elastase.

The mixed extract is nanoencapsulated, microencapsulated or nanosomes can be applied as a raw material in the form of a solution or powder.

In addition, the cosmetic composition containing the extract of the extract and the conical extract, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, essence, It may be applied in any one formulation selected from the group consisting of a pack, a soap, a shampoo, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder and an eye shadow.

The cosmetic composition containing the extract of the light wood and cone is effective in improving skin wrinkles, in particular, the effect of inhibiting the elastase, MMP-1 production and promoting collagen biosynthesis. The cosmetic composition containing the extract of the light wood and conical extract is useful in the cosmetic composition for preventing skin aging through the wrinkle improvement effect.

The present invention relates to a cosmetic for wrinkle improvement using the collagen production promoting, MMP-1 production inhibition and elastase inhibitory effect of the extracts of the light wood and cone.

Ligustrum japonicum is an evergreen shrub belonging to the Oleaceae family and grows in the coastal lowlands of 150-1,100m above sea level in Japan, Taiwan, Jeju Island, and southern part of Korea. The object is clear. Leaves are large, leathery, broad ovate, broad oval or ovate long oval, acute or obtuse, original or basal, 3-10cm long, 2.5-4.5cm wide, with insignificant spots on the back and flat edges. It is 5-12㎜ long and red brown with round vein. Flowers bloom in July-August, flowers are white, abdominal flowers hang at the end of the branch, and the length and width are 5-12cm, respectively, and the calyx is flat or wavy at the edges, and the corolla is 5-6㎜ long, and the tube is slightly smaller than the lobes. It is long or the same and leans back and has 2 surgeries. Fruits mature in October, and the nucleus is ovate round, 7-10mm long, ripened in purple, and remains in winter. Branches have fine hairs, dense leaves, egg-shaped oval or slightly round, short-leafed, inflorescences with short hairs on the axial axis, 3-6cm long, called round leaf ore wood (var.rotundifolium BLUME). The fruit contained oleanic acid, mannitol, glucose, palmitic acid, stearic acid, oleic acid and linolenic acid. The skin contained oleanolic acid, acetyl oleanolic acid and ursolic acid, and the seeds contained 14.9% fatty oil. Fat oil contains 10.5% of palmitic acid and stearic acid and 80.5% of linoleic acid. Oleanolic acid has a mild, diuretic effect, and mannitol has a relaxing effect and contains a large amount of glucose. This is thought to be related to tonic action. Mine trees are abbreviated names and journey names are called Japanese Journeys, Journeys, Seoja Lines, Calligraphy, Dongcheongmok, Arbitration, Shooters, Seosil, and Rabsu. It is used for ornamental and medicinal purposes. It is used as herbal medicine in tonic, boil, medicinal spirit, bowel, each, and acupuncture in oriental medicine and private medicine.

Hemerocallis fulva is a perennial herb attached to Liliaceae. Elongated leaves sprout from the root and pointed ends. In summer, flower stems come out and yellow flowers resembling lilies bloom. Conical is known as a medicinal herb that stabilizes the mind and cures stress and depression. It is used as a cure for pulmonary tuberculosis, anemia, jaundice, constipation, and urinary pain. Rooted water kills Mycobacterium tuberculosis and has diuretic, anti-inflammatory and hemostatic effects on the outpost. Excellent detoxification.

In the cosmetic composition of the present invention, the light wood and conical mixed extract is composed of a weight ratio of 1:10 to 10: 1, preferably a weight ratio of 1: 1.

As the active ingredient, the light wood and conical extracts are contained in an amount of 0.005 to 50% by weight, and more preferably 0.01 to 20% by weight, based on the total weight of the composition. If it is contained below 0.005% by weight, the activity is weak, and when it exceeds 50% by weight, it is unstable in the formulation.

Mixture extract of the light wood and cone of the present invention inhibits the activity of elastase, an enzyme of elastin in the skin, and acts to suppress MMP-1 production. It also has a mechanism of action to improve skin wrinkles by promoting collagen biosynthesis.

The light wood extract and the conical extract of the present invention can be prepared as follows. The fruits, leaves, branches or cones of the hardwood are washed thoroughly with water and dried in the shade. The dried product is placed in an extraction container and extracted for a predetermined time at an appropriate temperature in an appropriate amount of the extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a solvent selected from water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, chloroform, hexane, acetonitrile, or a mixed solvent thereof may be used. Preferably, hydrothermal extraction is performed by adding ethanol. It is characterized by. The amount of the extraction solvent is 1 to 15 times, preferably 7 to 8 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The deposition temperature is maintained at about 40 to 70 ℃, more preferably at 60 ℃ is most effective in the prevention of rot, discoloration and odor. The deposition time is preferably maintained for 2 to 7 hours, preferably 3 hours.

The extract obtained by the deposition is left for 1 to 3 days, preferably 1 day, then filtered using a filter cloth and filter paper and dried with a rotary decompression evaporator to powder of extracts of coniferous fruits and leaves and branches and conical extracts. Get

The optimal mixing ratio of mixing the light wood extract and the conical extract of the present invention is that the light wood extract and the conical extract are mixed in a weight ratio of 1:10 to 10: 1, more preferably 1: 1. On the other hand, the mixed extract of the present invention may be extracted after mixing the light wood and cone, or may be mixed after obtaining each extract.

If present in the form of a solution or powder in the form of nanocapsules, microcapsules, nanosomes, etc. containing the extract and the extract of the conical extract, those skilled in the art will control the amount of the solution or powder in the composition according to the present invention within the concentration range Could be.

Cosmetics prepared with a cosmetic composition containing the extract of the light wood and conical extract as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible lotion. In addition to the cosmetics containing the extract of the light wood and conical extract of the present invention, by containing a dermatologically acceptable medium or base can be prepared in the form of adjuvants that can be applied topically or systemically applied commonly used in the dermatology field.

In addition, the cosmetic composition of the present invention, in addition to the mixture of mineral wood and conical extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, interfaces Common to active agents, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological field, such as any other ingredients used as. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

The cosmetic composition of the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo , Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder, eye shadow and the like can be applied.

[Example]

Hereinafter, the present invention will be described in detail with reference to the following examples and test examples, but the present invention is not limited to these examples.

Manufacturing example  1: Preparation of Mineral Wood Extract

After crushing the dried fruit and stem of the dry wood in the shade, 1kg of the mixture mixed at a weight ratio of 1: 1 with 10-fold 70% (V / V) ethanol aqueous solution was refluxed for 6 hours, and cooled. Filtration was carried out with a filter paper having a permeation size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower, and then completely dried using a reduced pressure dryer to obtain 29 g of light wood extract.

Manufacturing example  2: Preparation of Conical Extract

After grinding the roots of dried cones in the shade, 1kg was refluxed for 6 hours with 10-fold 70% (V / V) ethanol aqueous solution and cooled down, and then filtered through a filter paper having a permeate size of 8 μm. The filtered extract was concentrated under reduced pressure at 60 ° C. or lower and completely dried using a reduced pressure dryer to obtain 18 g of light wood extract.

The dilution solution prepared by diluting the light wood extract and the conical extract prepared in Preparation Examples 1 and 2, and the mixture of 1: 1, respectively, in dimethyl sulfoxide for each concentration was used in the experiment.

Test Example  One: Elastase  Control activity test

10 ul of a mixture of test sample and elastase enzyme solution (1,000 units / mL, Sigma, USA) mixed with 2.5 wt% agar containing 0.1 wt% Elastin-Congo red (Sigma, USA) and reacted at room temperature for 10 minutes After injection, the cells were incubated at 37 ° C. for 18 hours. After the incubation period, when the zona pellucida is formed around the Elastin-Congo red agar by the activity of the elastase, the effect of inhibiting the elastase activity can be measured by measuring its diameter (halo).

Elastase alone is added dropwise to the agar medium containing elastin, while 0.01, 0.05, 0.1 and 0.5% solution of the extract of the light wood and 0.01, 0.05, 0.1 and 0.5% solution of the conical extract and a 1: 1 weight ratio of these extracts. A sample of 0.01, 0.05, 0.1 and 0.5% solution was added dropwise together with elastase to measure the diameter of halo on the medium to determine the elastase inhibitory activity of the extract from the mineral wood. Was compared dropwise with elastase.

The test results were obtained as shown in Table 1 below.

sample
density
(%) Halo diameter (cm) Mineral wood
extract Conical
extract Mineral Tree Extract + Conical Extract Elastase Retinol
crystal 0.01 1.5 1.6 1.3 1.8 0.9 0.05 1.4 1.4 1.1 0.10 1.1 1.1 0.8 0.50 1.1 1.1 0.8

The larger the halo diameter, the greater the degradation of elastin. As can be seen in Table 1 above, the halo diameter is excellent when the extracts of the oak extract, the conical extract and the mixed extract thereof are reduced. Elastase inhibitory effect was found, it was found that there is a synergistic effect when treated with a mixture of ores and conical extract compared to the case of each extract treatment.

Test Example  2: Collagen biosynthesis promoting effect test

Fibroblasts were inoculated at a cell concentration of 5 × 10 ⁵ in IMDM medium containing 10% FBS and incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator. After 24 hours of incubation, the light wood extract and the cone extract prepared in Preparation Examples 1 and 2, and the mixture of 1: 1 mixed each of them were diluted in dimethyl sulfoxide so as to have a final concentration of 50, 100, 500, and 1000 µg / ml. The diluted solution was added to give a stress to the cells by UV irradiation for 1 hour after incubation under the same conditions for 18 hours. Thereafter, the fibroblasts were recovered using Trizol reagent (invitrogen, USA), mRNA was extracted, and cDNA was synthesized through a series of processes. The amount of gene expression was confirmed by electrophoresis by amplifying the PROCOLLAGEN TYPE I gene region from the synthesized cDNA. Gene amplification was performed using a thermal cycler (GenePro, Hangzhou bioer tech. pmol primer (F: AGC CAG CAG ATC GAG AAC AT, R: TCT TGT CCT TGG GGT TCT TG), mixed with taq polymerase and adjusted to 50 uL with distilled water 95 ° 5 min 2 cycles / 72 degrees 1 minutes 28 cycles amplification, was carried out under the conditions of reacting for 5 minutes at 72 degrees. The expression rate of the produced procollagen was calculated according to the following equation. As a control, normal fibroblasts without treatment of the sample and UV irradiation were used.

Procollagen Expression (%) = B / A × 100 (%)

A: amount of procollagen expression in the control group

B: Procollagen Expression Levels of Sample Treatment and UV Irradiated Fibroblasts

The test results were obtained as shown in Table 2 below.

Sample concentration
(Μg / ml) procollagen expression rate (%) Light wood extract Conical Extracts Mineral Tree Extract + Conical Extract 0 2.62 2.62 2.62 50 26.32 18.42 42.26 100 49.12 38.86 59.75 500 83.10 72.88 98.86 1000 117.47 104.62 142.68

As shown in the results of Table 2, the sample applied by mixing the light wood and cone extract was higher collagen biosynthesis promoting effect related to the skin wrinkles, than the case of applying the light extract and cone extract alone, the synergistic effect is better Showed.

Test Example  3: inhibitory effect of MMP-1 production

Fibroblasts (Korean Cell Line Bank, Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc. Denmark) to 1st 10 ⁶ cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. After incubating for 24 hours at dimethyl sulfoxide so that the final concentration of 50, 100, 500, 1000 ㎍ / ㎖ of the light wood extract and conical extract prepared in Preparation Examples 1 and 2, and a mixture of 1: 1, respectively The diluted solution prepared by diluting the seed was added, and then cultured in serum-free DMEM medium for 48 hours. After incubation, the supernatant of each well was collected and the amount of the newly synthesized MMP-1 (µg / ml) was measured using an MMP-1 assay kit (Amersham, USA), and the inhibition rate of MMP-1 production was determined according to the following equation. It was calculated and the results are shown in Table 3 below. At this time, TGF-β (10 mg / ml, Roche, United States) was used as a positive control of MMP-1 production inhibition rate.

MMP-1 Production Inhibition Rate (%) = (quantity of MMP-1 in 1-test group / amount of MMP-1 in control group) × 100

Sample concentration
(Μg / ml) % Inhibition of MMP-1 production Light wood extract Conical Extracts Mineral Tree Extract + Conical Extract TGF-β 50 17.2 14.8 30.5 42.2 100 20.9 26.5 60.5 65.5 500 47.6 38.8 68.5 69.8 1000 57.1 47.5 86.5 88.8

As shown in the results of Table 3, the sample applied by mixing the light and extract extracts of the light wood and cone extract alone had a higher MMP-1 production inhibition effect, and the synergistic effect of the control TGF- The effect was similar to β.

Test Example  4: cytotoxicity test

Fibroblasts were inoculated at a cell concentration of 5 × 10 ⁵ in IMDM medium containing 10% FBS and incubated for 24 hours in a 37 ° C., 5% CO ₂ incubator. After incubation, the medium was removed, and the mixture of the light wood extract and the conical extract prepared in Preparation Examples 1 and 2, and 1: 1, respectively, was added to dimethyl sulfoxide so that the final concentration was 50, 100, 500, and 1000 µg / ml. After diluting the prepared dilution solution and incubating for 24 hours, 100 μl of MTT (3- [4,5-dimethylthiazol-2yl] -2,5-diphenyltetrazolium boromide, Sigma, USA) solution was added to each well. (3 mg / ml) was further incubated for 4 hours. Then, the supernatant was removed, 150 μl of dimethyl sulfoxide (DMSO) was added, and shaking for 30 minutes to dissolve the generated formazan, and the absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the following formula and the results are shown in Table 4 below.

Cell viability (%) = absorbance of the sample addition group / absorbance of the control group × 100

Sample concentration
(Μg / ml) Cell survival rate (%) Light wood extract Conical Extracts Mineral Tree Extract + Conical Extract 0 100 100 100 50 100 100 100 100 100 100 100 500 100 100 100 1000 94.76 95.43 95.12

As shown in the results of Table 4, all of the samples applied by mixing the light extract, cone extract, and the extract of the light and cone extract was confirmed that there is no cytotoxicity at a concentration of 500 ㎍ / ㎖, the cell viability at a concentration of 1000 ㎍ / ㎖ Although this decreases, it is confirmed that there is no problem in safety.

Example  One: emulsion  Base manufacturer

The emulsion base was prepared according to the composition of Table 5 below for clinical trials on the skin wrinkle improvement effect and skin safety of the cosmetic containing the extract of the extract of the present invention and conical cone.

Comparative example  1, 2: emulsion  Base manufacturer

As shown in the following Table 5, an emulsion base containing only the light wood extract or not containing the extract of the present invention was prepared as a comparative example.

ingredient weight% Example 1 Comparative Example 1 Comparative Example 2 glycerin 3.00 3.00 3.00 Disodium ID 0.02 0.02 0.02 Polyglyceryl-3-methylglucose distearate 1.50 1.50 1.50 Cetearyl Alcohol 0.50 0.50 0.50 Caprylic / capric triglyceride 7.00 7.00 7.00 Polyacrylamide & C13-14 Isoparaffin & Laures-7 0.60 0.60 0.60 Light wood extract 1.00 1.00 - Conical Extracts 1.00 - - Incense, preservative a very small amount a very small amount a very small amount Purified water Balance Balance Balance system 100 100 100

Test Example  5: skin wrinkle improvement

Skin wrinkle improvement effect of the cosmetic composition containing the light wood and conical extract of the present invention was measured in 30 healthy women aged 35 to 50 years. First, it was divided into three groups, Example 1 in Group A, Comparative Example 1 in Group B, and the formulation of Comparative Example 2 in Group C were applied twice a day (morning and evening) on the face for 3 months. After 3 months, the degree of wrinkle improvement was evaluated by subject's questionnaire and image analysis of wrinkles.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in Table 6 below.

Image analysis of wrinkles was performed by taking a replica of the eye under the eye before the experiment was started, and replicas immediately after the experiment was completed at the same area under the eye. Was measured. The measurement results of wrinkle density by image analysis are shown in Table 7 below as a reduction rate for wrinkle density compared to before use.

sample No improvement Minor improvements Moderate improvement A significant improvement Example 1 One One 4 4 Comparative Example 1 2 3 4 One Comparative Example 2 7 3 - -

sample Wrinkle Density Reduction (%) Example 1 49 Comparative Example 1 33 Comparative Example 2 14

From Tables 6 and 7, it can be seen that the skin wrinkle improvement effect of Example 1, which contains the mixed mixture of light wood and cone, is very excellent.

Test Example  6: Skin safety test

In order to confirm the skin safety of the cosmetic composition containing the mineral extract of the present invention and the extract from the conical extract, there has never been a hypersensitivity reaction to skin irritation in the past, and it is aimed at 20 women in their 20s and 30s who do not currently have skin disease or skin allergy symptoms. Human skin patch test was performed. The test site was first wiped with 70% ethanol and then dried. The materials of Example 1 and Comparative Examples 1 and 2 were added dropwise into the Fin chamber (Finn chamber, 100x 10, EPITEST, Finland) by 15 µg, and then sealed in the forearm inner part of the test subject. The patch was patched for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. The skin response was determined according to the regulations of the Internatiolnal Contact Dermaitis Research Group (ICDRG), and the average response rate was calculated according to the following formula, and the results are shown in Table 8.

Average skin reactivity = (score × number of responses × 100 × 1/2) × [3 (maximum score) × total number of subjects (n)]

* Evaluation criteria and score of skin reaction

Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe erythema and alveoli, crust formation

Test substance 1 hour later 24 hours later Responsiveness (%)
(n = 20) ± + ++ ± + ++ Example 1 - - - - - - 0.00 Comparative Example 1 - - - - - - 0.00 Comparative Example 2 - - - - - - 0.00

As can be seen from Table 8, there was no skin irritation in the cosmetics containing the extracts of the light wood and the cone, so that skin stability was excellent.

Test Example  7: Stability Test of Formulation

The stability test for the cosmetic containing the extract of the light wood and conical extract of the present invention was carried out by the following method. In order to test the stability of the cosmetics Example 1 and Comparative Examples 1 and 2 were kept in an opaque glass container in a constant temperature chamber maintained at 45 ℃ constant 12 kinds and stored in a completely shaded refrigerator kept at 4 ℃ constant After storing for 12 weeks in an opaque glass container, discoloration and odor, and the degree of separation was measured and compared the results are shown in Table 9 below. At this time, the product discoloration, odor and separation degree was classified into six grades and evaluated.

0: No change 1: Very little separation (color change, odor)

2: Slightly separated (discolored, decolored) 3: Slightly separated (discolored, decolored)

4: Severe separation (discoloration, odor) 5: Extremely severe separation (discoloration, odor)

division Formulation Stability Example 1 Comparative Example 1 Comparative Example 2 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0 0 0 0 0 0 Deodorant 0 0 0 0 0 0 detach 0 0 0 0 0 0

As can be seen from Table 9, Examples 1 and Comparative Examples 1 and 2 were stable at almost 45 ° C. and 4 ° C. without discoloration, discoloration, or separation, and the oak extract and the conical extract were stable in the formulation. Indicates that it exists.

Cosmetic compositions containing the extract of the light wood and cone of the present invention can be prepared in the following various formulations.

Example  2: flexible lotion

In the composition shown in Table 10 below, the flexible lotion containing the extract of the light wood and the cone was prepared by a conventional method.

ingredient Content (% by weight) Mineral wood extract 0.5 Conical Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  3: nutrition lotion ( Milk Croissant )

To a nutritive lotion containing a light wood and conical extract in the composition shown in Table 11 was prepared by a conventional method.

ingredient Content (% by weight) Mineral wood extract 0.6 Conical Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 20 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  4: nutrition cream

In the composition shown in Table 12 to prepare a nutritious cream containing the extract of the bark and the conical in a conventional manner.

ingredient Content (% by weight) Mineral wood extract 1.5 Conical Extract 1.5 Lipophilic monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Preservative, scent, pigment a very small amount Purified water Balance Sum 100

Example  5: Massage  cream

Massage creams containing the extracts of the bark and the conical extracts were prepared in a conventional manner according to the composition shown in Table 13.

ingredient Content (% by weight) Mineral wood extract 0.5 Conical Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  6: pack

The pack containing the oak and cone extract with the composition shown in Table 14 below was prepared by a conventional method.

ingredient Content (% by weight) Mineral wood extract 0.5 Conical Extract 0.5 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Claims (7)

Wrinkle improvement cosmetic composition containing a mixed extract of mineral wood and conical as an active ingredient. The method of claim 1, The mixed extract is a cosmetic composition for improving wrinkles, characterized in that the extract of light wood and conical extract consists of a weight ratio of 1:10 to 10: 1. The method of claim 1, The mixed extract is anti-wrinkle cosmetic composition, characterized in that nanoencapsulated, microencapsulated or nanosomes. The method of claim 1, The composition is wrinkles cosmetic composition for improving wrinkles, characterized in that to improve wrinkles by promoting collagen production. The method of claim 1, The composition for improving wrinkles, characterized in that to improve wrinkles by inhibiting the elastase activity and MMP-1 production. 6. The method according to any one of claims 1 to 5, Wrinkle cosmetic composition for improving wrinkles, characterized in that the mixed content of the light wood and cone is contained 0.005 to 50% by weight based on the total weight of the composition. 6. The method according to any one of claims 1 to 5, The composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, pack, soap, shampoo, cleansing foam, cleansing lotion , Cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow, wrinkles cosmetic composition for improving wrinkles, characterized in that consisting of any one selected from the group consisting of.