KR20110036299A - Cosmetic composition containing malted wheat extract for anti-aging and whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition containing nuruk extract is provided to ensure antiaging and whitening and to suppress collagen decompression. CONSTITUTION: A cosmetic composition for antiaging and whitening contains 0.001-90 weight% of nuruk extract. A method for preparing the Prunusp mume extract comprises: a step of adding water or organic solvent to Prunusp mume; a step of refluxing and precipitating; a step of filtering and centrifuging; a step of separating residual and liquid; and a step of compressing the liquid.

Description

Cosmetic composition containing malted wheat extract for anti-aging and whitening}

The present invention relates to a composition containing the yeast extract as an active ingredient, and relates to a composition that exhibits an anti-aging and whitening effect such as collagen degradation by containing an extract of the yeast itself.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays reaching the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness, wrinkles, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and blemishes, freckles, and mushrooms also increase.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are constituting skin, appear, and are subjected to oxidative stress caused by free radicals and active harmful oxygen. As skin ages or the skin is exposed to ultraviolet light, cyclooxygenase-2 (Cox-2), an enzyme that produces proinflammatory cytokine, known to cause inflammation in most of the cells that make up skin, , the biosynthesis of cyclooxygenase increases, the biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, increases NO (nitric oxide) by inducible nitric oxide synthase (iNOS) Production is known to increase. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight As a result, the decomposition and degeneration of the substrate are accelerated, and the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, a lot of research is being conducted on the active ingredient that can prevent and improve the phenomenon of aging.

On the other hand, a number of factors are involved in determining the skin color of the human, including the activity of melanocytes (melanocytes) that make melanin pigment, the distribution of blood vessels, the thickness of the skin and the presence or absence of pigments, such as carotenoids, bilirubin, etc. Factors are important.

Especially, the most important factor is a black pigment called melanin which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of this melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion, stress, and environmental factors such as ultraviolet irradiation.

Melanin pigment, which is produced from melanocytes of the skin of the body, is a phenolic polymer material having a complex form of black pigment and protein. It protects skin organs below the dermis by blocking ultraviolet rays from the sun and free radicals generated in skin It plays a useful role in protecting proteins and genes in the skin.

As described above, melanin produced by stress stimulation inside and outside the skin is a stable substance that does not disappear until it is discharged to the outside through skin keratinization even if the stress disappears. However, if more melanin is produced than necessary, it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results. In addition, as the leisure population increases, the number of people who enjoy being active outside increases the demand to prevent melanin pigmentation caused by ultraviolet rays.

In response to such demands, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof, or substances having tyrosinase inhibitory activity have been used in combination with cosmetics or medicines. Its use is limited due to insufficient whitening effect, safety problems on skin, formulation and stability problems when formulated in cosmetics, and the like.

The inventors of the present invention, through repeated and various experiments, confirmed that the yeast extract can provide a safe and sufficient anti-aging and whitening effect on the skin, and completed the present invention.

Accordingly, an object of the present invention is to provide an anti-aging and whitening composition containing yeast extract.

In order to achieve the above object, the present invention provides a cosmetic composition and health food composition containing a yeast extract as an active ingredient.

The composition according to the present invention exhibits an anti-aging effect and suppresses the production and degradation of collagen, and thus can be variously used in cosmetics, health food or medicine.

The present invention relates to a composition containing the yeast extract as an active ingredient. The leaven used in the present invention generally means leaven used for making makgeolli (Takju). The method for preparing the yeast extract is not particularly limited, and may be obtained by extracting and filtering the yeast to obtain a filtrate, and then concentrating it.

The yeast extraction process of the present invention can be carried out by conventional methods known in the art. For example, water or an organic solvent may be added to Nuruk, reflux extraction is performed, and the residue and the filtrate are separated by filtration and centrifugation. The separated filtrate is concentrated under reduced pressure to obtain a Nuruk extract.

The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and preferably 80% ethanol is used. At this time, the extraction temperature is preferably 10 ~ 80 ℃, the extraction time is preferably 6 ~ 24 hours. If the extraction temperature and the extraction time is out of the extraction efficiency may be lowered or a change of components may occur.

After obtaining the extract using the solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying.

Nuruk extract used as an active ingredient in the present invention promotes the production of collagen to provide an anti-aging effect by mitigating the reduction of collagen caused by aging of the human skin. In addition, the yeast extract may provide an excellent skin whitening effect by inhibiting the production of melanin.

The composition of the present invention may be an external composition for skin, and more specifically, may be an anti-aging and whitening composition. The content of the yeast extract contained in the composition is not particularly limited. Specifically, the yeast extract of the present invention may be contained in an amount of 0.001 to 90% by weight based on the total weight of the composition, preferably 0.01 to 30% by weight, more preferably 0.1 to 10% by weight. The content range of the yeast extract is to select a preferred range that can improve the anti-aging effect and whitening effect.

When formulating the external composition for skin according to the present invention in the form of cosmetics, such as softening cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack It may be formulated in a form, the formulation is not particularly limited.

In addition, the composition according to the present invention may be a health food composition or a pharmaceutical composition.

The health food composition may be formulated in various forms such as powders, granules, tablets, capsules and drinks.

In addition, the said health food composition can be mix | blended with 1 type (s) or 2 or more types of the following additives as needed. As an additive, For example, various fruits (concentrated juice, powder juice, etc.), such as grapefruit, an apple, an orange, a lemon, a pineapple, a banana, a pear, may be used; Vitamins (remithol palmitate, riboflavin, pyridoxine, cyanocobalamine, sodium ascorbate, nicotinic acid amide, calcium pantothenate, folic acid, biotin, cholecalciferol, cholinic acid choline, tocopherol, β Water-soluble and fat-soluble vitamins such as carotene); Flavorings (lemon flavor, orange flavor, strawberry flavor, grapefruit flavor, vanilla essence, etc.); Amino acids, nucleic acids and salts thereof (glutamic acid, sodium glutamate, glycine, alanine, aspartic acid, sodium aspartate, inosinic acid, etc.); Plant fibers (polydextrose, pectin, xanthan gum, glucomannan, alginic acid, etc.); Or minerals (sodium chloride, sodium acetate, magnesium sulfate, potassium chloride, magnesium chloride, magnesium carbonate, calcium chloride, dipotassium phosphate, monosodium phosphate, glycerol calcium phosphate, ferrous citrate sodium, ammonium ferric citrate, iron citrate, Manganese sulfate, copper sulfate, sodium iodide, potassium sorbate, zinc, manganese, copper, iodine, cobalt, and the like.

The pharmaceutical composition may further contain pharmaceutical aids such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts for regulating osmotic pressure and / or buffers, and other therapeutically useful substances, and various oral agents in accordance with conventional methods. Or in parenteral dosage forms.

Formulations for oral administration include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, granules, etc. These formulations may contain diluents (e.g., lactose, dextrose, Sucrose, mannitol, sorbitol, cellulose and glycine), lubricants such as silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols. Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt Pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners. Tablets may be prepared by conventional mixing, granulating or coating methods. In addition, formulations for parenteral administration include external skin preparations, injections and the like, ointment is preferred as an external preparation for skin, may be an isotonic aqueous solution or suspension in the injection formulation.

Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dose of the drug according to the present invention depends on various factors such as the progression of symptoms, onset time, age, health condition, complications, etc. of the subject to be administered. Although based on an adult, generally 1 to 500 mg / kg of the composition, preferably 30 to 200 mg / kg, which is combined in the above-mentioned weight ratio, may be administered once or twice a day. Dosage does not limit the scope of the invention in any way.

The content of the Nuruk extract in the health food composition or pharmaceutical composition is not particularly limited, but may be contained in the range of 10 to 90% by weight, preferably 20 to 50% by weight based on the total weight of the composition. This is in consideration of the fact that the content of the powder and the functional ingredient may be 10 to 60% by weight in the manufacture of tablets and soft capsules, and the content of the powder and the functional ingredient may be 10 to 90% by weight in the manufacture of hard capsules.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Yeast Extract

5 L of an 80% ethanol aqueous solution was added to 1 kg of commercial Nuruk, extracted under reflux three times, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 50 g of yeast extract.

Reference Example 1 Comparison of Kojic Acid and Yeast Extract

Kojic acid and koji extract of Example 1 were measured by HPLC (High Performance Liquid Chromatography, Varian, USA) in order to compare kojiic acid (kojic acid, purchased through YM chemical) and the yeast extract of the present invention. Comparing the HPLC results of kojic acid and koji extract by applying the same analysis conditions, it is possible to determine the presence or absence of kojic acid component. The results are shown in FIGS. 1 and 2.

As can be seen in Figures 1 and 2, the koji extract and kojic acid has a difference in the position where the peak (peak), respectively. Through this, the yeast extract used in the present invention does not have kojic acid, and it can be confirmed that they are different materials from each other.

Test Example 1 Type I Procollagen Assay

Human normal fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then the Nuruk extract obtained in Example 1 was replaced with a medium containing 1 ppm, 10 ppm, 50 ppm. As a positive control, one containing TGF-β (Human Transforming Growth Factor-β1, Roche Co.) at a concentration of 10 ng / ml was used, and one containing no yeast extract and TGF-β was used as a negative control. Cells were harvested on the third day of culture to quantify the amount of type I procollagen produced by ELISA. The amount of procollagen of the negative control group was set to 0 and the amount of procollagen of the positive control group was set to 100, and the comparison with each measured value was calculated. The results are shown in Table 1 below.

division Type I procollagen biosynthesis (%) Negative control group 0 Yeast Extract (1ppm) 32.7 Leak Extract (10ppm) 40.8 Yeast Extract (50ppm) 41.5 TGF-β (10ng / ml) 100

As can be seen in Table 1, the Nuruk extract in the normal human fibroblast monolayer culture system promoted type I procollagen production in a concentration-dependent manner compared to the negative control. Therefore, the yeast extract of the present invention can be confirmed that by promoting collagen production to alleviate the reduction of collagen caused by aging of the human skin can provide an excellent anti-aging effect.

Test Example 2 Measurement of Melanin Inhibition Effect Using Pigment Cells

Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) in DMEM with 10% fetal placental serum, 100nM 12-O-tetradecanoyl Incubated at 37 ° C. and 5% CO ₂ in a medium to which phorbol (tetradecanoylphorbol) -13-acetate and 1 nM cholera toxin were added. The cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, the cells were incubated at a concentration of 10 ⁵ cells / well in 24-well plates, and then each test material was added for 3 consecutive days. And incubated. As a test substance, hydroquinone and the Nuruk extract of Example 1 were used at a concentration of 10 ppm. At this time, the hydroquinone was used as a positive control group. Then, the culture medium was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and the melanin production inhibition rate was calculated according to Equation 1 below. Method).

Test substance Melanin production inhibition rate (%) Untreated group 100 Yeast Extract 37.4 Hydroquinone (positive control) 41.1

As shown in Table 2, it can be confirmed that the yeast extract of the present invention shows melanin production inhibition similar to that of hydroquinone, which is a known whitening substance.

Test Example 3 Irritation Test

In order to compare the usability of kojic acid, a known whitening substance, and the yeast extract used as an active ingredient in the present invention, the degree of irritation such as stinging, itching, and eye aspiration in 15 panel sensitive to irritation such as stinging, burning, etc. Was tested.

Subjects were rubbed with kojic acid (purchased through kojic acid, YM chemical) and 0.5ml each of the yeast extract of the present invention, randomly changing the left and right, and scored between 0 and 3.0 by 0.1 point unit. The results are shown in Table 3 below.

<Evaluation Criteria>

0 to 0.4: no stimulation

0.5 to 1.0: slightly irritating

1.1 to 2.0: moderate stimulation

2.1 to 3.0: severe irritation

Kojic acid Yeast Extract Stinging 0.85 0.19 Burning 0.50 0.50 Average 0.68 0.26

As can be seen in Table 3, in the case of kojic acid, there was some degree of tingling, burning, there was a sense of irritation that can usually be felt slightly. On the other hand, the yeast extract used in the present invention has a slight irritation in hotness, but stinging is hardly felt, indicating that there is no irritation on average.

Therefore, the yeast extract used in the present invention can be confirmed that there is no irritation as a substance different from kojic acid can provide better usability.

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but merely to be described in detail.

Formulation Example 1 Preparation of Soft Capsule

Yeast extract 80mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, vegetable hardened oil 8mg, lead 4mg and lecithin 9mg were mixed and mixed according to a conventional method to prepare a soft capsule filling solution. 400 mg per capsule was filled to prepare a soft capsule. In addition, a soft capsule sheet was prepared at a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerine, and 10 parts by weight of sorbitol solution and filled with the filling solution, thereby preparing a soft capsule containing 400 mg of the composition according to the present invention.

Formulation Example 2 Preparation of Tablet

Yeast extract 80mg, vitamin E 9mg, vitamin C 9mg, galactooligosaccharide 200mg, lactose 60mg and malt sugar 140mg were mixed, granulated using a fluidized bed dryer, and then 6mg sugar ester was added. Tablets were prepared by compression of 504 mg of these compositions in a conventional manner.

Formulation Example 3 Preparation of Drink

Nuruk extract 80mg, vitamin E 9mg, vitamin C 9mg, glucose 10g, citric acid 0.6g, and 25g of liquid oligosaccharides were mixed and 300ml of purified water was added to each bottle to 200ml. After filling the bottle sterilized for 4 to 5 seconds at 130 ℃ to prepare a beverage.

Formulation Example 4 Preparation of Granules

The yeast extract 80mg, vitamin E 9mg, vitamin C 9mg, anhydrous glucose of 250mg and starch 550mg were mixed, molded into granules using a fluidized bed granulator, and then filled into sachets.

Formulation Example 5 Nutritional Cosmetics

Nutritional longevity was prepared according to the conventional composition described in Table 4 below.

ingredient Content (% by weight) Yeast Extract 5.0 Squalane 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 6 Nutrition Cream

Nutritional cream was prepared in a conventional manner according to the composition described in Table 5.

ingredient Content (% by weight) Yeast Extract 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 Cured Castor Oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 7 Massage Cream

To prepare a massage cream in a conventional manner according to the composition shown in Table 6.

ingredient Content (% by weight) Yeast Extract 5.0 Beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 PEG60 Cured Castor Oil 2.0 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

[Formulation Example 8] Pack

To prepare a pack in a conventional manner according to the composition described in Table 7.

ingredient Content (% by weight) Yeast Extract 5.0 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Quantity Purified water Balance Sum 100

Formulation Example 9 Ointment in External Skin Preparation

The ointment was prepared in a conventional manner according to the composition described in Table 8.

ingredient Content (% by weight) Yeast Extract 5.0 Purified water Balance glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Stearyl alcohol 1.0 antiseptic Quantity incense Quantity Pigment Quantity beeswax 4.1 Sum 100

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

Figure 1 shows the results of the yeast extract and kojic acid measured by HPLC

Figure 2 shows the measurement results for koji acid and koji extract by overlapping the results of FIG.

Claims (5)

Cosmetic composition containing yeast extract as an active ingredient. The cosmetic composition of claim 1, wherein the yeast extract is contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for anti-aging. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for whitening. Health food composition containing yeast extract as an active ingredient.

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