KR20110019803A - Composition of lactic acid - Google Patents

Abstract

PURPOSE: A cleanser of powdery Lactobacillus containing flavor for preventing and treating periodontal diseases is provided to suppress the growth of Streptococcus mutans and P. gingivalis. CONSTITUTION: A cleanser of powdery Lactobacillus for preventing and treating periodontal diseases contains powdery Lactobacillus to be directly administered to a lesion. The powdery Lactobacillus contains 0.1-50 weight% of flavor selected from grape flavor, strawberry flavor, orange flavor, cheery flavor, vitamin flavor, pepper mint flavor, coffee flavor, and cinnamon flavor. The powdery Lactovacillus is Lactobacillus acidophilus, Bifidobacterium longum SPM1205, Pediococcus pentosaceus, or Bacillus polyfermenticus.

Description

Lactobacillus powder cleaning agent for preventing and treating periodontal disease with added fragrance {composition of lactic acid}

The present invention relates to a lactic acid bacteria powder cleaning agent for preventing and treating periodontal disease with added flavor, and more particularly, exhibits the activity of inhibiting the growth of Streptococcus mutans bacteria causing caries and causing periodontitis. Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis (Pediococcus pentosaceus) contains any one or more lactic acid bacteria selected as an active ingredient and is formed as a powder of lactic acid bacteria to be applied directly to the affected part of the periodontal disease, the grape flavor, strawberry flavor, orange Taste, Cherry, Vitamin, Mint, Coffee, Cinnamon and Nurungji It relates to a lactic acid bacteria powder cleaning agent for the prevention and treatment of periodontal disease with added flavor, characterized in that the flavor is mixed 0.1 to 50% by weight.

Certain lactic acid bacteria produce antibacterial substances (Bacteriocin, hydrogen peroxide) that can inhibit the growth of S. mutans, which causes tooth decay, and P. gingivalis, which causes periodontitis. In addition, certain lactic acid bacteria compete for the nutrients necessary for the growth of these pathogenic bacteria, preventing them from growing well. Periodontal disease is a bacterial-related inflammatory disease of the supportive tissue of the tooth, which is a nonspecific and reversible inflammation of the gum tissue from relatively mild form of gingivitis to aggressive form of periodontitis that destroys the supportive tissue of the tooth. Belongs to that category.

Periodontitis is associated with lower gum infection of specific gram-negative bacterial aggregates, which destroys periodontal tissue. Tooth decay is one of the most common diseases of the teeth called dental caries. It is an infectious bacterial disease in which food in the mouth is rotted by acid-resistant streptococci and lactic acid bacteria and the components of teeth are destroyed by acid. In Korea, the prevalence of tooth decay is about 80%, and one person has an average of two to three caries. Cause of tooth decay has been found to work in combination, but the most important is plaque. Plaque is mixed with various bacteria, especially acid-resistant streptococci and lactic acid bacteria, among which Streptococcus mutans is known as the main caries. Such Streptococcus mutans grow by attaching to the surface of the teeth to produce acid using sugar in the food to corrode the teeth and at the same time invade tooth bone cells to cause various alveolar bone erosion and gum disease.

On the other hand, since the tooth is destroyed once it is not regenerated, the tooth decay is sealed and treated with a material replacing the tooth. Current methods to prevent tooth decay are to reduce sugar intake through brushing and proper diet, or to prevent acid production by caries by using xylitol. In addition, there is a method of forming a material that is not soluble in acid using fluorine. This method prevents tooth decay but limits the fluorine penetrating layer to penetrate deeply on the tooth surface and easily disappear. Such a method has no effect of killing caries-causing bacteria or preventing bone cell invasion.

Lactic acid bacteria are used in the production of food products such as dairy products, kimchi, brewed foods that prevent the growth of pathogens and harmful bacteria by lactic acid produced by lactic acid fermentation. In addition, it is an important bacterium that lives in the intestine of mammals and prevents abnormal fermentation by various germs, which enhances the immune function of the host and increases cholesterol in the intestine.

The possibility of preventing cardiovascular disease by inhibiting absorption has also been reported, and strains having antiviral activity against avian influenza virus among the lactic acid bacteria isolated from kimchi have also been found. Based on these results, the lactic acid bacteria found in kimchi have attracted attention as a probiotic because of their ease of ingestion and ease of application of food additives.

The present invention is Lactobacillus acidophilus (Bactobacillus acidophilus), Bifidobacterium, which is a strain that produces a natural antimicrobial substance of stable physical properties that are harmless to the human body, easy to consume, and can effectively inhibit the growth of caries-causing bacteria. An object of the present invention is to provide a method for treating periodontal disease prepared from any one or more lactic acid bacteria selected from Bifidobacterium longum SPM1205 and Pediococcus pentosaceus. In addition, the present invention is a periodontal disease prepared from any one or more lactic acid bacteria selected from Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) It is an object of the present invention to provide an antimicrobial composition capable of preventing and treating tooth decay with an antimicrobial agent used in the treatment method.

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. One or more lactic acid bacteria selected from Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus are included as active ingredients, and It is formed of lactic acid bacteria in powder form so that it can be directly applied to the affected part, and the lactic acid bacteria powder is selected from grape flavor, strawberry flavor, orange flavor, cherry flavor, vitamin flavor, peppermint flavor, coffee flavor, cinnamon flavor and nurungji flavor flavor. It relates to a lactic acid bacteria powder cleaning agent for the prevention and treatment of periodontal disease, which is mixed 0.1 to 50% by weight The.

According to the present invention, Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus are selected from any one or more lactic acid bacteria prepared from lactic acid bacteria. The prophylactic and therapeutic lactic acid complex has specific and excellent antimicrobial activity against periodontal disease, minimizes the problems and side effects caused by antibiotic treatment in the treatment of periodontal disease, and can be safely taken for a long time to prevent and treat periodontal disease. It is effective to

The treatment method of periodontal disease using lactic acid bacteria according to the present invention can be treated by implanting the chip containing the lactic acid bacteria in the affected area or by injecting the lactic acid bacteria reagent directly into the affected area, and deep periodontal sac after tooth scaling and tooth addressing It can be treated by directly inserting a mixture of lactic acid bacteria, and periodontitis treatment can be applied as an alternative to treatment due to antibiotics or treatment with non-antibiotics.Alternative therapy using lactic acid bacteria for periodontal disease can be used. Can be.

The method of treatment of periodontal disease using lactic acid bacteria according to the present invention directly acts lactic acid bacteria competitively with the causative bacteria of periodontal disease, effectively inhibit the growth of harmful bacteria, make the oral environment healthier and have the resistance effect seen in antibiotics. There is no need to worry and the lactic acid bacteria used in the treatment method of the present invention are harmless to the human body, so there is no need to worry about overdose or side effects.

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. Lactobacillus ashophilus, Bifidobacterium longum SPM1205, Pediococcus pentosaceus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascense mescicus One or more lactic acid bacteria selected from terricus, Bacillus rickeniformis, Bacillus polyfermentus, Bacillus subtilis, Cycaromyces cerevises and Clostridium butyricum can be directly applied to the affected area of periodontal disease. It is formed as a lactic acid bacteria in powder form so that the lactic acid bacteria powder, grape flavor, strawberry flavor Lactobacillus powder for the prevention and treatment of periodontal disease with added flavor, characterized in that the flavor is selected from 0.1 to 50% by weight of flavor, orange flavor, cherry flavor, vitamin flavor, peppermint flavor, coffee flavor, cinnamon flavor and Nurungji flavor flavor It relates to a cleaning agent.

In the lactic acid bacteria powder for the prevention and treatment of periodontal disease of the present invention, lactic acid bacteria are Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus). It is characterized in that any one or more lactic acid bacteria selected.

In the lactic acid bacteria powder cleaning agent for preventing and treating periodontal disease of the present invention, the lactic acid bacteria powder is characterized in that any one form selected from the one formed by the lactic acid bacteria powder is embedded in the interior of the container.

The lactic acid bacteria powder used in the present invention is a lactic acid bacterium that inhibits the growth of Streptococcus mutans that causes tooth decay and produces a culture solution that exhibits stable antibacterial activity at neutral pH. The lactic acid bacteria that can be used in the present invention can use all of the probiotic preparations currently in use, and Lactobacillus ashidophyllus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascium, Bacillus mesentericus, Bacillus liquenipo It can be selected and used from Miss, Bacillus polyfermentus, Bacillus subtilis, Cicaro myces cerevise, and Clostridium butyricum.

Lactobacillus powder used in the present invention, Lactobacillus acidophilus, Bifidobacterium longum (Bifidobacterium longum) SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) to use any one selected from Preferred, stick-like, no movement, the colony is milky white.

Lactobacillus acidophilus used in the present invention generates organic acids such as lactic acid, produces unique bacteriocins, and has an excellent inhibitory effect on pathogens, and has a function of lowering blood cholesterol in humans. It has been evaluated as the most useful strain in Lactobacillus because it is excellent in biological immune response and anti-cancer effect.

Bifidobacterium used in the present invention is a non-pathogenic Gram-positive, anaerobic bacterium that balances the intestinal microflora and improves lactose resistance, decreases serum cholesterol levels, increases vitamin synthesis, inhibits carcinogenesis, enhances immune function, and enhances cancer. It is known to have a beneficial effect on the human body, such as prevention. Bifidobacterium longum SPM1205 used in the present invention is a Bifidobacterium strain that acts to activate the intestinal function of the human body and at the same time enhance the immune function.

Pediococcus sp. Strain used in the present invention is a microbial strain that prevents microbial contamination and Pediococcus pentosaceus effectively prevents microbial contamination that may occur during the fermentation process of microorganisms. It is a lactic acid bacterium widely used as livestock probiotics, functional toilet papers, functional foods and nutrients, functional feed additives, environmental improvers, and various enzyme preparations as it can prevent secondary decay and odors and prevent pathogens. Pediococcus sp. Strain used in the present invention is preferably Pediococcus pentosaceus, Pediococcus pentosaceus (pediococcus pentosaceus) ST-01 strain desirable. The Pediococcus pentosaceus strain is named Pediococcus pentosaceus ST-01. Pediococcus pentosaceus ST-01 used in the present invention prevents contamination of microorganisms and effectively prevents secondary contamination of microorganisms including Escherichia coli (Escherichia coli). It may include.

The lactic acid bacteria used in the present invention are composed of the isolation and identification of lactic acid bacteria having antimicrobial activity, and furthermore, the antimicrobial activity test against caries causing bacteria of lactic acid bacteria according to the present invention. A spot-on-the-lawn test was performed to investigate the antimicrobial activity of lactic acid bacteria against isolated streptococcus mutans. The culture medium of Streptococcus mutans was plated in BHI agar medium, and the concentrated supernatant of the lactic acid bacteria according to the present invention was inoculated thereon. While culturing this, the formation of an inhibition zone of Streptococcus mutans is observed. It can be seen that early growth of Streptococcus mutans is inhibited by the antibacterial activity of lactic acid bacteria. As the strain used in the present invention, Streptococcus mutans standard strain (KCTC3065) was used, and lactic acid bacteria having antibacterial activity against Streptococcus mutans were used.

Lactic acid bacteria powder to be used in the present invention is a grape flavor, strawberry flavor, orange flavor, cherry flavor, vitamin flavor, peppermint flavor, coffee flavor, cinnamon flavor and Nurungji flavor flavor is mixed 0.1 to 50% by weight, 1 to It is preferable to mix 3 weight%. When the lactic acid bacteria powder used in the present invention is mixed with less than 0.1% by weight of the fragrance does not show the efficacy of the aroma occurs in the refrigeration of lactic acid bacteria, and when the fragrance is mixed by 50% by weight, the efficacy in lactic acid bacteria is reduced.

[Example 1] Confirmation of the antimicrobial activity of the concentrated culture supernatant against caries

The antimicrobial activity of Streptococcus mutans bacteria known as causative causative bacteria by the culture supernatant of lactic acid bacteria according to the present invention was examined through the following method.

(1) Target microorganism selection

To measure antimicrobial activity, Streptococcus mutans KCTC3065 strain was distributed in the Gene Bank of Korea Research Institute of Bioscience and Biotechnology. The target bacteria were inoculated in BHI liquid medium and incubated at 37 ° C.

(2) Inoculation of target bacteria and analysis of antibacterial activity

The test strain was inoculated in 5 ml BHI liquid medium and precultured at 37 ° C. for one day, and main culture was performed in 5 ml BHI liquid medium at 37 ° C. from OD ₆₀₀ to 0.2, and 100 μl of BHI solid medium was smeared. Sterile 6 mm filter paper disks were dropped onto the smeared solid medium, and the culture supernatant concentrated at 30-fold and the pH was adjusted to 7.0. . The antimicrobial activity was examined by observing the degree of inhibition ring formation around the disk.

Nisin is a fermentation product of Streptococcus lactis, a bacteriocin that is widely used as an antiseptic for food preservatives in the United States and other countries. In this example, it was used as a control to compare the antimicrobial activity by lactic acid bacteria.

Antibacterial substances produced by lactic acid bacteria have been shown to inhibit the growth of streptococcus, the causative agent of caries. The concentrated culture supernatant of lactic acid bacteria whose pH was adjusted to 7.0 was also observed to inhibit the growth of caries. Nisin has been shown to inhibit the growth of cavities, but the degree of antibacterial activity is weaker than the activity of lactic acid bacteria according to the present invention.

The antimicrobial material produced by the lactic acid bacteria according to the present invention has been confirmed that the physical properties are stable and have direct antimicrobial activity in the growth of caries and inhibit the growth of caries-inducing bacteria better than the previously used antimicrobial substance, nisin.

[Example 2] Antibacterial activity according to the growth stage of the decayed bacteria

Streptococcus mutan, which is the causative agent of tooth decay, by the lactic acid bacteria of the present invention

The effects of different stages of growth were examined in the following ways. The antimicrobial substance of lactic acid bacteria was used 30 times concentrated culture supernatant. 100 μl of Streptococcus mutans KCTC3065 main culture solution having a value of 0.2 at OD ₆₀₀ was dispensed into a 96 well plate. Concentrated culture supernatant of the lactic acid bacteria of the present invention was treated with 10 ㎕ to each well dispensed with caries. The culture supernatant of the lactic acid bacteria prepared in the above manner was adjusted to pH 7.0 with 3 M Tris buffer, and 10 µl each was added to each well in which 100 µl of the caries was dispensed. The 96 well plate containing the cavities to which a certain amount of concentrated culture supernatant was added was incubated at 37 ° C., and the turbidity of the cavities was measured at 580 nm at 1 hour intervals. As a result, the growth of caries was inhibited by lactic acid bacteria over time. All experimental groups were found to inhibit the growth of Streptococcus mutans KCTC3065. In particular, it was confirmed that the concentrated culture supernatant of lactic acid bacteria which did not adjust pH was superior to antibacterial activity than nisin, and had an effective antibiotic effect on caries by inhibiting the growth of caries from early stages.

Example 3 Preparation of Lactic Acid Bacteria Powder Cleaner for Treatment of Periodontal Disease

Lactobacillus ashophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus, any one selected from 2g lactic acid bacteria powder and 0.2g grape flavor Prepared. Absorbent lactic acid bacteria powder was dissolved in purified water and used as a cleaning agent. Periodontitis can be treated by washing with absorbent lactobacillus powder in a deep periodontal sac after tooth scaling, periodontal palpation, or root canal lubrication.

Example 7 Treatment of Periodontal Disease Using Lactic Acid Bacteria Powder Cleaner

To treat periodontal disease with lactic acid bacterium powder cleaning agent, after absorbing tooth toothing, periodontal rupture, or root root blotting, the absorbent lactic acid powder stored in FIG. 1 is directly inserted into the deep periodontal sac or the absorbent lactic acid powder stored in FIG. Periodontitis was treated by dissolving in purified water. In addition, the treatment was performed by directly inserting a mixture of lactic acid bacteria powder into the deep periodontal sac after tooth scaling, tooth addressing, or root canal lubrication.

The treatment method of periodontal disease using lactic acid bacteria powder cleaning agent according to the present invention can be treated with lactic acid bacteria in periodontal disease. The treatment method of periodontal disease using the lactic acid bacteria powder cleaning agent according to the present invention can be treated by directly inserting a mixture of lactic acid bacteria powder stored in FIG. The method of treating periodontal disease using the lactic acid bacteria powder cleaning agent according to the present invention can be applied as an alternative to the treatment of periodontitis caused by antibiotics or to the treatment with non-antibiotics. It can be used as a therapy.

Treatment method of periodontal disease using lactic acid bacteria powder according to the present invention, lactic acid bacteria directly competitive with the causative bacteria of periodontal disease, effectively inhibit the growth of harmful bacteria, make the oral environment more healthy and resistant effect seen in antibiotics There is no need to worry. Since the lactic acid bacteria used in the present invention are harmless to the human body, there is no need to worry about overdose or side effects.

1 is a perspective view showing a state in which the flavor-added lactic acid bacteria powder is embedded in the container.

DESCRIPTION OF THE REFERENCE NUMERALS

50: container 52: lid

Claims (3)

Lactobacillus ash for the prevention and treatment of periodontal disease that can inhibit the growth of S. mutans causing caries and inhibit the growth of P. gingivalis that causes periodontitis Lactobacillus acidophilus, Bifidobacterium longum SPM1205, Pediococcus pentosaceus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascium, Bacillus mecethelis Powder so that any one or more lactic acid bacteria selected from Bacillus rickenformis, Bacillus polyfermentus, Bacillus subtilis, Cyclomyces cerevises and Clostridium butyricum can be directly applied to the affected area of periodontal disease. It is formed as a lactic acid bacterium, grape flavor, strawberry flavor, orange to the lactic acid powder Flavor, cherry mathyang vitamin mathyang, peppermint mathyang, mathyang coffee, cinnamon mathyang scorched and lactic acid powder cleaning agent for prevention and treatment of a flavor, characterized in that a mixture of 0.1 to 50 wt.% Fragrance is selected from the mathyang added periodontal disease. According to claim 1, wherein the lactic acid bacteria Lactobacillus acidophilus (Lactobacillus acidophilus), Bifidobacterium longum (Bifidobacterium longum) SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) is at least one selected from Lactic acid bacteria powder cleaning agent for the prevention and treatment of periodontal disease, characterized by the addition of flavor. According to claim 1, wherein the lactic acid bacteria powder is formed in the form of a strip of absorbent lactic acid bacteria powder by hard tableting the lactic acid bacteria powder in the form of a strip; Hard tableting the lactic acid powder in the form of a chip and formed in the form of an absorbent lactic acid powder chip; Lactic acid powder is impregnated into the absorbent gelatin sponge to be formed into the absorbent lactic acid powder gelatin sponge form; Lactic acid powder is coated on the surface of the absorbent gelatin sponge to form a absorbent lactic acid powder coated gelatin sponge; Lactobacillus powder and the absorbent gelatin sponge is mixed to form a hard tableted absorbent lactic acid powder gelatin sponge strip; Lactobacillus powder for the prevention and treatment of periodontal disease with added flavor, characterized in that the lactic acid powder is formed in the form of a capsule embedded in the interior of the capsule, and the lactic acid bacteria powder is formed in the interior of the container detergent.

Images