KR20110017534A

KR20110017534A - Composition of lactic acid

Info

Publication number: KR20110017534A
Application number: KR1020090075047A
Authority: KR
Inventors: 신미정
Original assignee: 신미정
Priority date: 2009-08-14
Filing date: 2009-08-14
Publication date: 2011-02-22

Abstract

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. Lactobacillus is contained as an active ingredient, and it can be applied directly to the affected area of periodontal disease to treat periodontitis by inserting it directly into deep periodontal sac after tooth scaling, tooth addressing, or root canal lubrication. How to; A method of treating the lactic acid bacteria complex by implanting it in the affected area; And it relates to a method for treating periodontal disease using the lactic acid bacteria complex, characterized in that any one of the methods selected from the method of preparing the lactic acid bacteria complex with a lactic acid bacteria reagent and directly injected into the affected area.

Periodontal disease, prevention and treatment, lactic acid bacteria complex

Description

Treatment of periodontal disease using lactobacillus complex {composition of lactic acid}

The present invention relates to a method of treating periodontal disease using a lactic acid bacterium complex, and more particularly, P. gingivalis, which exhibits the activity of inhibiting the growth of S. mutans, which causes tooth decay, and causes periodontitis. Lactobacillus is included as an active ingredient for the prevention and treatment of periodontal disease that can inhibit the proliferation of teeth. A method of treating periodontitis by directly inserting it into a deep periodontal sac after curettage or root resection; A method of treating the lactic acid bacteria complex by implanting it in the affected area; And it relates to a method for treating periodontal disease using the lactic acid bacteria complex, characterized in that any one of the methods selected from the method of preparing the lactic acid bacteria complex with a lactic acid bacteria reagent and directly injected into the affected area.

Certain lactic acid bacteria produce antibacterial substances (Bacteriocin, hydrogen peroxide) that can inhibit the growth of S. mutans, which causes tooth decay, and P. gingivalis, which causes periodontitis. In addition, certain lactic acid bacteria compete for the nutrients necessary for the growth of these pathogenic bacteria, preventing them from growing well. Periodontal disease is a bacterial-related inflammatory disease of the supportive tissue of the tooth, an aggressive form of periodontitis that destroys the supportive tissue from a relatively mild form of gingivitis with nonspecific and reversible inflammation of the gum tissue. Far belongs to that category.

Periodontitis is associated with lower gum infection of specific gram-negative bacterial aggregates, which destroys periodontal tissue. Tooth decay is one of the most common diseases of the teeth called dental caries. It is an infectious bacterial disease in which food in the mouth is rotted by acid-resistant streptococci and lactic acid bacteria and the components of teeth are destroyed by acid. In Korea, the prevalence of tooth decay is about 80%, and one person has an average of two to three caries. Cause of tooth decay has been found to work in combination, but the most important is plaque. Plaque is mixed with various bacteria, especially acid-resistant streptococci and lactic acid bacteria, among which Streptococcus mutans is known as the main caries. Such Streptococcus mutans grow by attaching to the surface of the teeth to produce acid using sugar in the food to corrode the teeth and at the same time invade tooth bone cells to cause various alveolar bone erosion and gum disease.

On the other hand, since the tooth is destroyed once it is not regenerated, the tooth decay is sealed and treated with a material replacing the tooth. Current methods to prevent tooth decay are to reduce sugar intake through brushing and proper diet, or to prevent acid production by caries by using xylitol. In addition, there is a method of forming a material that is not soluble in acid using fluorine. This method prevents tooth decay but limits the fluorine penetrating layer, which does not penetrate deep into the tooth surface and is easily lost. Such a method has no effect of killing caries-causing bacteria or preventing bone cell invasion.

Lactic acid bacteria are used in the production of food products such as dairy products, kimchi, brewed foods that prevent the growth of pathogens and harmful bacteria by lactic acid produced by lactic acid fermentation. In addition, it is an important bacterium that lives in the intestine of mammals and prevents abnormal fermentation by various germs, which enhances the immune function of the host and increases cholesterol in the intestine.

The possibility of preventing cardiovascular disease by inhibiting absorption has also been reported, and strains having antiviral activity against avian influenza virus among the lactic acid bacteria isolated from kimchi have also been found. Based on these results, the lactic acid bacteria found in kimchi have attracted attention as a probiotic because of their ease of ingestion and ease of application of food additives.

The present invention is Lactobacillus acidophilus (Bactobacillus acidophilus), Bifidobacterium, which is a strain that produces a natural antimicrobial substance of stable physical properties that are harmless to the human body, easy to consume, and can effectively inhibit the growth of caries-causing bacteria. An object of the present invention is to provide a method for treating periodontal disease prepared from any one or more lactic acid bacteria selected from Bifidobacterium longum SPM1205 and Pediococcus pentosaceus. In addition, the present invention is a periodontal disease prepared from any one or more lactic acid bacteria selected from Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) It is an object of the present invention to provide an antimicrobial composition that can prevent and treat tooth decay with an antimicrobial agent used in the treatment method as a quasi-drug of the treatment method of periodontal disease.

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. One or more lactic acid bacteria selected from Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus are included as active ingredients, and The present invention relates to a lactic acid bacteria complex for preventing and treating periodontal disease, which is formed of a lactic acid bacteria complex in solid form or powder form so as to be directly applied to the affected area.

According to the present invention, Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus are selected from any one or more lactic acid bacteria prepared from lactic acid bacteria. The prophylactic and therapeutic lactic acid complex has specific and excellent antimicrobial activity against periodontal disease, minimizes the problems and side effects caused by antibiotic treatment in the treatment of periodontal disease, and can be safely taken for a long time to prevent and treat periodontal disease. It is effective to

The treatment method of periodontal disease using lactic acid bacteria according to the present invention can be treated by implanting the chip containing the lactic acid bacteria in the affected area or by injecting the lactic acid bacteria reagent directly into the affected area, and deep periodontal sac after tooth scaling and tooth addressing It can be treated by directly inserting a mixture of lactic acid bacteria, and periodontitis treatment can be applied as an alternative to treatment due to antibiotics or treatment with non-antibiotics.Alternative therapy using lactic acid bacteria for periodontal disease can be used. Can be.

The method of treatment of periodontal disease using lactic acid bacteria according to the present invention directly acts lactic acid bacteria competitively with the causative bacteria of periodontal disease, effectively inhibit the growth of harmful bacteria, make the oral environment healthier and have the resistance effect seen in antibiotics. There is no need to worry and the lactic acid bacteria used in the treatment method of the present invention are harmless to the human body, so there is no need to worry about overdose or side effects.

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. Lactobacillus ashophilus, Bifidobacterium longum SPM1205, Pediococcus pentosaceus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascense mescicus It contains 0.1 to 10 g of each of the lactic acid bacteria at least one selected from terricus, Bacillus rickeniformis, Bacillus polyfermentus, Bacillus subtilis, Cycaromyces cerevises and Clostridium butyricum Lactobacillus in solid or powder form for direct application to affected areas of periodontal disease A method of treating periodontitis by inserting the complex directly into a deep periodontal pocket after performing tooth scaling and addressing or root ablation; A method of treating the lactic acid bacteria complex by implanting it in the affected area; And it relates to a method for treating periodontal disease using the lactic acid bacteria complex, characterized in that any one of the methods selected from the method of preparing the lactic acid bacteria complex with a lactic acid bacteria reagent and directly injected into the affected area.

The present invention is to suppress the growth of S. mutans bacteria causing tooth decay and to prevent and treat periodontal disease that can inhibit the growth of P. gingivalis that causes periodontitis. Lactobacillus ashophilus (Lactobacillus acidophilus), Bifidobacterium longum (Bifidobacterium longum) SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) at least one selected from lactic acid bacteria contained 0.1 to 10g each active ingredient And it relates to a lactic acid bacteria complex for the prevention and treatment of periodontal disease, characterized in that formed in a solid or powdered lactic acid complex to be administered directly to the affected part of the periodontal disease.

The lactic acid bacteria complex of the present invention is formed in the form of absorbent lactic acid bacteria (strip) by hard tableting the lactic acid bacteria in the form of a strip; Hard tableting the lactic acid bacteria in the form of chips formed in the form of absorbent lactic acid bacteria (chip); Lactic acid bacteria are impregnated into the absorbent gelatin sponge to form an absorbent lactobacillus gelatin sponge; Lactic acid bacteria are coated on the surface of the absorbent gelatin sponge to form an absorbent lactobacillus coated gelatin sponge; Lactic acid bacteria and absorbent gelatin sponges are mixed to form a hard tableted absorbent lactic acid bacteria gelatin sponge strip; And lactic acid bacteria is characterized in that any one form selected from those formed in the form of a capsule embedded in the interior of the capsule.

The lactic acid bacteria used in the present invention are lactic acid bacteria that inhibit the growth of Streptococcus mutans causing tooth decay and produce a culture solution that exhibits stable antibacterial activity at neutral pH. The lactic acid bacteria that can be used in the present invention can use all of the formal probiotics currently used, Lactobacillus ashidophyllus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascium, Bacillus mesentericus, Bacillus rickenformis , Bacillus polyfermentus, Bacillus subtilis, Cycarmyces cerevisiae, and Clostridium butyricum can be used.

As the lactic acid bacteria used in the present invention, it is preferable to use any one selected from Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus. It is rod-shaped and there is no movement and colony is milky white.

Lactobacillus acidophilus used in the present invention generates organic acids such as lactic acid, produces unique bacteriocins, and has an excellent inhibitory effect on pathogens, and has a function of lowering blood cholesterol in humans. It has been evaluated as the most useful strain in Lactobacillus because it is excellent in biological immune response and anti-cancer effect.

Bifidobacterium used in the present invention is a non-pathogenic Gram-positive, anaerobic bacterium that balances the intestinal microflora and improves lactose resistance, decreases serum cholesterol levels, increases vitamin synthesis, inhibits carcinogenesis, enhances immune function, and enhances cancer. It is known to have a beneficial effect on the human body, such as prevention. Bifidobacterium longum SPM1205 used in the present invention is a Bifidobacterium strain that acts to enhance the immune function while activating the intestinal function of the human body.

Pediococcus sp. Strain used in the present invention is a microbial strain that prevents microbial contamination and Pediococcus pentosaceus effectively prevents microbial contamination that may occur during the fermentation process of microorganisms. It is a lactic acid bacterium widely used as livestock probiotics, functional toilet papers, functional foods and nutrients, functional feed additives, environmental improvers, and various enzyme preparations as it can prevent secondary decay and odors and prevent pathogens. Pediococcus sp. Strain used in the present invention is preferably Pediococcus pentosaceus, Pediococcus pentosaceus (pediococcus pentosaceus) ST-01 strain desirable. The Pediococcus pentosaceus strain is named Pediococcus pentosaceus ST-01. Pediococcus pentosaceus ST-01 used in the present invention prevents contamination of microorganisms and effectively prevents secondary contamination of microorganisms including Escherichia coli (Escherichia coli). It may include.

The lactic acid bacteria used in the present invention are composed of the isolation and identification of lactic acid bacteria having antimicrobial activity, and furthermore, the antimicrobial activity test against caries causing bacteria of lactic acid bacteria according to the present invention. A spot-on-the-lawn test was performed to investigate the antimicrobial activity of lactic acid bacteria against isolated streptococcus mutans. The culture medium of Streptococcus mutans was plated in BHI agar medium, and the concentrated supernatant of the lactic acid bacteria according to the present invention was inoculated thereon. While culturing this, the formation of an inhibition zone of Streptococcus mutans is observed. It can be seen that early growth of Streptococcus mutans is inhibited by the antibacterial activity of lactic acid bacteria. As the strain used in the present invention, Streptococcus mutans standard strain (KCTC3065) was used, and lactic acid bacteria having antibacterial activity against Streptococcus mutans were used.

[Example 1] Confirmation of the antimicrobial activity of the concentrated culture supernatant against caries

The antimicrobial activity of Streptococcus mutans bacteria known as causative causative bacteria by the culture supernatant of lactic acid bacteria according to the present invention was examined through the following method.

(1) Target microorganism selection

To measure antimicrobial activity, Streptococcus mutans KCTC3065 strain was distributed in the Gene Bank of Korea Research Institute of Bioscience and Biotechnology. The target bacteria were inoculated in BHI liquid medium and incubated at 37 ° C.

(2) Inoculation of target bacteria and analysis of antibacterial activity

The test strain was inoculated in 5 ml BHI liquid medium and precultured at 37 ° C. for one day, and main culture was performed in 5 ml BHI liquid medium at 37 ° C. from OD ₆₀₀ to 0.2, and 100 μl of BHI solid medium was smeared. Sterile 6 mm filter paper disks were dropped onto the smeared solid medium, and the culture supernatant concentrated at 30-fold and the pH was adjusted to 7.0. . The antimicrobial activity was examined by observing the degree of inhibition ring formation around the disk.

Nisin is a fermentation product of Streptococcus lactis, a bacteriocin that is widely used as an antiseptic for food preservatives in the United States and other countries. In this example, it was used as a control to compare the antimicrobial activity by lactic acid bacteria.

Antibacterial substances produced by lactic acid bacteria have been shown to inhibit the growth of streptococcus, the causative agent of caries. The concentrated culture supernatant of lactic acid bacteria whose pH was adjusted to 7.0 was also observed to inhibit the growth of caries. Nisin has been shown to inhibit the growth of cavities, but the degree of antibacterial activity is weaker than the activity of lactic acid bacteria according to the present invention.

The antimicrobial material produced by the lactic acid bacteria according to the present invention has been confirmed that the physical properties are stable and have direct antimicrobial activity in the growth of caries and inhibit the growth of caries-inducing bacteria better than the previously used antimicrobial substance, nisin.

[Example 2] Antibacterial activity according to the growth stage of the decayed bacteria

Streptococcus mutan, which is the causative agent of tooth decay, by the lactic acid bacteria of the present invention

The effects of different stages of growth were examined in the following ways. The antimicrobial substance of lactic acid bacteria was used 30 times concentrated culture supernatant. 100 μl of Streptococcus mutans KCTC3065 main culture solution having a value of 0.2 at OD ₆₀₀ was dispensed into a 96 well plate. Concentrated culture supernatant of the lactic acid bacteria of the present invention was treated with 10 ㎕ to each well dispensed with caries. The culture supernatant of the lactic acid bacteria prepared in the above manner was adjusted to pH 7.0 with 3 M Tris buffer, and 10 µl each was added to each well in which 100 µl of the caries was dispensed. The 96 well plate containing the cavities to which a certain amount of concentrated culture supernatant was added was incubated at 37 ° C., and the turbidity of the cavities was measured at 580 nm at 1 hour intervals. As a result, the growth of caries was inhibited by lactic acid bacteria over time. All experimental groups were found to inhibit the growth of Streptococcus mutans KCTC3065. In particular, it was confirmed that the concentrated culture supernatant of lactic acid bacteria which did not adjust pH was superior to antibacterial activity than nisin, and had an effective antibiotic effect on caries by inhibiting the growth of caries from early stages.

Example 3 Preparation of Lactic Acid Bacteria Strip Form Composite for Treatment of Periodontal Disease

Lactobacillus ashophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus, one of the lactic acid bacteria 2g selected from the hard and horizontally compressed into a strip form It was prepared in the form of a strip of absorbent lactic acid bacteria of 0.5cm, length 1cm, thickness 0.2cm. Periodontitis can be treated by directly inserting an absorbent lactobacillus strip into a deep periodontal pocket after tooth scaling, periodontal palpation, or root root blotting.

Example 4 Preparation of Lactic Acid Bacteria Chip Form Composite for Treatment of Periodontal Disease

Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus, 2 g of any one of the lactic acid bacteria selected hardly in the form of chips It was prepared in the form of absorbent lactic acid bacteria chip having a width of 0.5 cm, a length of 5 cm, and a thickness of 0.2 cm. After cutting the absorbent lactobacillus chip to an appropriate size, after performing tooth scaling and tooth addressing or root ablation, the periodontitis can be treated by directly inserting the cut absorbent lactobacillus chip into the deep periodontal sac.

Example 5 Preparation of Absorbent Lactic Acid Bacteria Gelatin Sponge-like Composite for Treatment of Periodontal Disease

Lactobacillus ashophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) mixed with an aqueous solution mixed with any one of 2g lactic acid bacteria as shown in Figure 10 The absorbent gelatin sponge was soaked and dried to prepare an absorbent lactobacillus gelatin sponge in the form of an absorbent lactobacillus strip having a width of 0.5 cm, a length of 1 cm, and a thickness of 0.2 cm. Periodontitis can be treated by directly inserting an absorbent lactobacillus gelatin sponge into the deep periodontal sac after tooth scaling, tooth addressing, or root canal blotting.

Example 6 Preparation of Absorbent Lactic Acid Bacteria Gelatin Sponge-like Composite for Treatment of Periodontal Disease

Lactobacillus acidophilus, Bifidobacterium longum SPM1205 and Pediococcus pentosaceus 2 g of any one of the lactic acid bacteria selected from Lactobacillus acidophilus and the absorbent gelatin sponge shown in FIG. The tablet was hardly compressed and mixed to prepare an absorbent lactobacillus gelatin sponge in the form of an absorbent lactobacillus strip having a width of 0.5 cm, a length of 1 cm, and a thickness of 0.2 cm. Periodontitis can be treated by directly inserting an absorbent lactobacillus gelatin sponge into the deep periodontal sac after tooth scaling, tooth addressing, or root canal blotting.

Example 7 Treatment of Periodontal Disease Using Lactic Acid Bacteria

To treat periodontal disease with lactic acid bacteria, periodontitis was treated by direct insertion of absorbent lactobacillus strips into deep periodontal sac after tooth scaling, periodontal rupture, or root ablation. In addition, the treatment was performed by directly inserting a mixture of lactic acid bacteria into the deep periodontal sac after tooth scaling, dental addressing, or root canal shortening. In order to treat periodontal disease with lactic acid bacteria, periodontitis was treated by direct insertion of absorbent lactobacillus gelatin sponge into deep periodontal sac after tooth scaling, periodontal rupture, or root root blotting.

Treatment of periodontal disease using lactic acid bacteria according to the present invention can be treated with lactic acid bacteria to periodontal disease. As shown in FIG. 11, the method of treating periodontal disease using lactic acid bacteria according to the present invention can treat periodontitis by directly inserting an absorptive lactobacillus strip into a deep periodontal sac after tooth scaling and periodontal rupture or root resection. The treatment method of the present invention can be treated by implanting the chip containing the lactic acid bacteria to the affected area or by injecting the lactic acid bacteria reagent directly into the affected area. The treatment method of periodontal disease using lactic acid bacteria according to the present invention can be treated by directly inserting a mixture of lactic acid bacteria into the deep periodontal sac after tooth scaling and periodontal surgery or root root squeeze. The treatment method of periodontal disease using lactic acid bacteria according to the present invention can be applied as an alternative to the treatment of periodontitis caused by antibiotics or resistance to non-antibiotic, and use as an alternative therapy for periodontal disease using lactic acid bacteria Can be.

The method of treatment of periodontal disease using lactic acid bacteria according to the present invention directly acts lactic acid bacteria competitively with the causative bacteria of periodontal disease, effectively inhibit the growth of harmful bacteria, make the oral environment healthier and have the resistance effect seen in antibiotics. There is no need to worry. Since the lactic acid bacteria used in the present invention are harmless to the human body, there is no need to worry about overdose or side effects.

The lactic acid bacteria complex of the present invention may be prepared by hardly compressing the lactic acid bacteria in the form of a strip as shown in FIGS. 1 to 3. The octagonal structure as shown in Figure 1 is a structure that is easy to pick up with forceps, and the forward-facing structure as shown in Figure 3 is conveniently formed in the periodontal pocket. The lactic acid bacterium complex of the present invention may be manufactured by forming the absorbent lactic acid bacterium chip (chip) by hard tableting the lactic acid bacteria in the form of a chip as shown in FIG. 4, and may be used by cutting to an appropriate size when used. The lactic acid bacteria complex of the present invention is produced by lactic acid bacteria impregnated in the absorbent gelatin sponge as shown in Figure 5 or formed in the form of absorbent lactic acid bacteria gelatin sponge or as shown in Figures 6 to 7 is coated on the surface of the absorbent gelatin sponge absorbent lactic acid coated gelatin sponge It can be produced in the form of. The lactic acid bacteria complex of the present invention may be prepared by mixing the lactic acid bacteria and the absorbent gelatin sponge in the form of a hardly compressed absorbent lactobacillus gelatin sponge strip, and as shown in FIG. 8, the lactic acid bacteria may be formed in the capsule form embedded in the capsule. .

Absorbent gelatin sponge (ABSORBABLE GELATIN SPONGE) used in the present invention as shown in FIG. 10 is purified specially treated gelatin (NF) -white sponge and is commercially known as CUTANPLAST ABSORBABLE GELATIN SPONGE. have. Absorbent Gelatin Sponge Absorbent hemostatic agent that can be used in various areas can instantly hemostatic bleeding or venous bleeding from capillaries. The absorbent gelatin sponge may be used in a dry state depending on the condition of the surgical site or the operator's preference, or may be wetted with isotonic saline or thrombin solution. Absorbent gelatin sponge can be cut to the desired size and used as it is or dried and soaked in physiological saline or an appropriate concentration of thrombin solution. In the absorbent gelatin sponge used in the present invention, gelatin is a water-soluble protein obtained by hydrolyzing collagen, a bioprotein, and unlike synthetic polymers, it is not toxic, biodegradable, and can inhibit antigenicity. In addition, gelatin is known to have a function of activating the phagocytosis of the body's dietary cells to increase the immune function in the body during bacterial invasion of the body.

There are several types of absorbent gelatin sponges used in the present invention as shown in FIG. 10. For example, a cross-linked gelatin sponge disclosed in US Pat. No. 2,507,244, a sponge prepared by freeze-drying a gelatin and thrombin mixture disclosed in US Pat. No. 2,558,395. , Sponge freeze-dried gelatin and polyvinylpyrrolidone mixed solution shown in US Patent No. 3,325,366, sponge prepared by mixing gelatin and starch presented in US Patent No. 2,899,362, US Patent No. 3,122,479 and US Patent No. 2,602,042 Sponges made from the disclosed cellulose derivatives, sponges made from collagen micropowders or collagen fibers disclosed in U.S. Patent Nos. 3,471,598 and 3,368,911, and commodities made of sponges by mixing collagen and algin are commercially available. have. In the absorbent gelatin sponge used in the present invention, a sponge made of only gelatin itself is very fragile and easily dissolved unless crosslinked, so that a crosslinking agent is used to impart mechanical properties to the sponge.

1 is a perspective view showing a state in which the absorbent lactobacillus strip of the octagonal structure is packaged in a wrapping paper.

Figure 2 is a perspective view showing a state in which the absorbent lactobacillus strip is packaged in a wrapping paper.

Figure 3 is a perspective view showing a state in which the absorbent lactobacillus strip of the half-moon structure is packaged in a wrapping paper.

Figure 4 is a perspective view of the absorbent lactic acid bacteria chip.

5 is a cross-sectional view showing a cross section of an absorbent gelatin sponge.

Figure 6 is a cross-sectional view showing a cross-section of the lactic acid bacteria coating layer formed on the absorbent gelatin sponge.

Figure 7 is a cross-sectional view showing a cross-section of the lactic acid bacteria coating layer on the upper and lower portions of the absorbent gelatin sponge.

Figure 8 is a perspective view showing a state in which the lactic acid bacteria are embedded in the capsule.

9 is a perspective view showing a state in which the lactic acid bacteria are embedded in the container.

10 is a commercially available absorbent gelatin sponge product photo

11 is a photograph showing a state of treating periodontitis by directly inserting an absorptive lactobacillus strip into a deep periodontal sac after tooth scaling and periodontal rupture or root resection with a lactic acid bacterium according to the present invention.

10, 10-1, 10-2: absorbent lactic acid bacteria strip

20: absorbent lactic acid bacteria chip 30: absorbent gelatin sponge

30-1, 30-2: lactic acid bacteria coating layer 40: capsule

50: container 52: lid

100: wrapping film 110: wrapping paper

Claims

Lactobacillus ash for the prevention and treatment of periodontal disease that can inhibit the growth of S. mutans causing caries and inhibit the growth of P. gingivalis that causes periodontitis Lactobacillus acidophilus, Bifidobacterium longum SPM1205, Pediococcus pentosaceus, Lactobacillus bifidus, Streptococcus faecalis, Streptococcus fascium, Bacillus mecethelis At least one lactic acid bacterium selected from Bacillus rickeniformis, Bacillus polyfermentus, Bacillus subtilis, Cyclomyces cerevisiae and Clostridium butyricum is contained as an active ingredient of 0.1 to 10 g each and is used for periodontal disease. Lactobacillus complex in solid form or powder form for application directly to the affected area Ah after the periodontal scaling and curettage or apical surgery lubricant inserted directly into the deep pockets methods for the treatment of gingivitis; A method of treating the lactic acid bacteria complex by implanting it in the affected area; And Method of treating periodontal disease using the lactic acid bacteria complex, characterized in that made of any one method selected from the method of producing the lactic acid bacteria complex with a lactic acid bacteria reagent and directly injected into the affected area.

According to claim 1, wherein the lactic acid bacteria Lactobacillus acidophilus (Lactobacillus acidophilus), Bifidobacterium longum (Bifidobacterium longum) SPM1205 and Pediococcus pentosaceus (Pediococcus pentosaceus) is at least one selected from Method of treating periodontal disease using the lactic acid bacteria complex, characterized in that.

According to claim 1, wherein the lactic acid bacteria complex is formed in the form of absorbent lactic acid bacteria (strip) strip by hard tableting the lactic acid bacteria in the form of a strip; Hard tableting the lactic acid bacteria in the form of chips formed in the form of absorbent lactic acid bacteria (chip); Lactic acid bacteria are impregnated into the absorbent gelatin sponge to form an absorbent lactobacillus gelatin sponge; Lactic acid bacteria are coated on the surface of the absorbent gelatin sponge to form an absorbent lactobacillus coated gelatin sponge; Lactic acid bacteria and absorbent gelatin sponges are mixed to form a hard tableted absorbent lactic acid bacteria gelatin sponge strip; And lactic acid bacteria is a method of treating periodontal disease using the lactic acid bacteria complex, characterized in that any one selected from the form of a capsule formed in the interior of the capsule.