KR20110000050A - Composite of cosmetics contained chondroitin phellinus linteus, ganoderma lucidum and paecilomyces japonica extract - Google Patents

Abstract

PURPOSE: A composition containing mushroom extract is provided to ensure ABTS radical removal ability, SOD(superoxide dismutase)-like activity and electron donating ability. CONSTITUTION: A cosmetic composition contains hot water extract and ethanol extract of Phellinus Linteus, Ganoderma Lucidum, and Paecilomyces Japonica. The hot water extract is obtained by: adding 10 times volume of distilled water to Phellinus Linteus, Ganoderma Lucidum, and Paecilomyces Japonica, refluxing at 85°C for three hours; separating supernatant and precipitate; and repeating the process three times. The ethanol extract is obtained by: adding 10 times volume of 70% ethanol; precipitating at room temperature for 24 hours; and repeating three times. The mixture ratio of Phellinus Linteus, Ganoderma Lucidum, and Paecilomyces Japonica is 50:10:1(w/w/w).

Description

Composition of cosmetics contained chondroitin Phellinus linteus, Ganoderma lucidum and Paecilomyces japonica extract}

The present invention relates to a cosmetic composition containing a composite extract including a situation mushroom, Cordyceps sinensis, Ganoderma lucidum.

In this study, the present study examined the applicability of functional cosmetics by checking the antioxidant activity through the evaluation of its physiological activity by using S. mushroom, Cordyceps sinensis and Ganoderma lucidum.

The human desire to maintain beauty and youth by delaying or suppressing aging of the skin is getting bigger now that the desire for basic consciousness has been solved. Also, human desire for beauty continued to develop in various forms with history. The method includes a method of inhibiting or regulating metabolism in vivo, a method of using cosmetics, and the like. The regulation of metabolic mechanisms in vivo is intended to prevent aging by controlling free radicals and cellular damage, which are closely related to the process of aging (MKYang, J. Kor . Soc . Cosm ., 9, p78-82, 2003). ).

Recently, efforts to develop various natural products and chemicals having antioxidant activity as health foods or medicines have been increasing. Various methods for measuring antioxidant activity have been developed. The DPPH free radical scavenging activity assay was first introduced in 1958 (MS Blois, Nature , 181, pp1199-1200, 1958) is a very simple way to measure antioxidant activity using the degree of change in color. Many researchers are still measuring antioxidant activity using this method. DPPH is a relatively stable compound among radicals and is colored purple in EtOH. However, when encountering a substance with antioxidant activity, the antioxidant active substance depletes the radicals of DPPH and loses its purple color, and the value decreases during UV measurement (H. Matsumura, K. Oka, Contact Dermatitis , 33 , p231, 1995). In addition, the method has an advantage of being highly related to the actual antioxidant activity, and the electron donating action is used as a measure of inhibiting lipid oxidation by donating electrons to the active radical, and also used as a measure of inhibiting aging by the active radical. (W. Aberer, KE Anderson, et al., Contact Dermatitis , 28 , p1, 1993).

Representative reactive oxygen, which is a problem in vivo, is produced from enzymes, reduction metabolism, chemicals, pollutants and photochemical reactions from the base triplet oxygen, a stable molecular state widely present in nature. Its products include superoxide, hydrogen peroxide, hydroxyl radical, singlet oxygen, and active oxygen, which kills macrophages and removes old proteins in vivo. It is an essential substance used in the body and the like, but its reactivity may show harmful effects in vivo (AE Favier, J. Cadet, et al., Birkhauser Verlag , 83 , p 118, 1995). Too much free radicals can cause fatal damage to individual parts of the body (LT Churchill, British). Medical Bulletin , 49 (3) , pp 481-193, 1993). Because of this, it is known to cause various diseases and aging such as cancer, stroke, heart disease, skin disease, digestive disease, inflammation, rheumatism, autoimmune disease (Kim Chang-jin et al., New Material Search, Free Academy, pp325-350, 1996). Ahn Bong Jeon, Jin Tae Lee, Aging and Cosmetics, Kwangmungak, 4, 2000). Antioxidants that remove or inhibit free radicals can be used as treatments for various diseases and as skin aging inhibitors.

In July 2001, the Korea Food and Drug Administration (KFDA) issued a list of functional cosmetic standards and test methods. The substances that help whitening are listed as a noticeable ingredient, and the extracts of oil-soluble licorice, and retinol and adenosine are listed as substances that help to improve wrinkles of skin (Cosmetics Policy, Food and Drug Administration, pp. 243). -250, 2003). In addition, natural products such as green tea, baekbaekpi, betel nut, golden, and wild ginseng are used as cosmetic ingredients (Kim Young-Joong, Recent Trends in Natural Products Research, Journal of Korean Cosmetic Society, 29, pp1-15, 2003; , Journal of the Korean Society of Cosmetic Scientists, 29, pp79-87, 2003)

Whitening cosmetic material containing an extract of natural phellinus (Whitening cosmetic material containing an extract of natural Phellinus Linteus)-relates to a whitening cosmetic containing natural extracts of natural phellinus mushroom, more specifically, a fine powder of natural pumice mushroom having a predetermined strength The natural mushroom mushroom powder was subjected to ultra-high frequency treatment at the same time under reduced pressure in a specific solvent, followed by sterilization, discoloration, and odor removal to efficiently obtain the extract of the mushroom mushroom. To prevent whitening or pigmentation of the skin by ultraviolet rays by preparing a whitening cosmetic compound containing the above ultraviolet absorber or ultraviolet scattering agent and peptide, licorice extract, arbutin, rice extract, vitamin C, etc. By suppressing melanin production such as skin tone, skin tone, blemishes and freckles Should improve the course, the present invention relates to a whitening cosmetic containing a mushroom extract natural conditions that have an antioxidant effect on the skin, anti-aging function by (10-0345382-0000).

A cosmetic composition containing mixing extract of Sipjangsaeng- containing natural products (pine sprouts, ganoderma lucidum, bilberry, deer antler, grow, germanium, royal jelly, red ginseng, salmon roe and lemon) related natural substance (Pinussylvestris, Ganoderma lucidum Karst., blueberry, Cervus elaphus L., Amyda sinensis, Germanum, Apismellifera L., Panax ginseng CAMeyer, salmon egg and Citrus Medica Limonum) and the method of manufacturing thereof)- The 10 natural products associated with it, namely PINUS SYLVESTRIS, GANODERMA LUCIDUM KARST., CERVUS ELAPHUS L., PANAX GINSENG CA MEYER, CITRUS MEDICA LIMONUM, ZAMARA (AMYDA SINENSIS) ), Germanium (GERMANUM), Royal Jelly (APIS MELLIFERA L.), Bilberry (VACCINIUM VITIS-IDAEA) and Salmon (ONCHORHYNCHUS PETS), Pineus SYLVESTRIS, GANODERMA LUCIDUM KARST., Crustus (CERVUS) ELAPHUS L.), red ginseng (PANAX) GINSENG CA MEYER) and lemon (CITRUS MEDICA LIMONUM) are extracted through supercritical fluid extraction techniques and mixed with the rest of natural products to cope with wrinkle relief, elasticity and moisturizing. (10-0580858-0000).

Cosmetic Composition Containing Shiitake Mushroom Extract and / or Cordyceps Sinensis Extract-A cosmetic composition, comprising: Cordyceps sinensis extract as a cell growth promoting component; And / or oligo lentinane obtained by acid treatment of lentinan extracted from shiitake mushrooms (10-0372359-0000).

As such, many natural products have been researched as cosmetic materials, and in particular, various herbal cosmetics applying herbal medicines used in oriental medicine have been researched and produced. The present invention continues to search for cosmetic ingredients with excellent stability among plant extracts such as herbal medicines, vegetables, fruits, flowers, etc., which are used as herbal medicine, used in women, diseases and degenerative diseases for a long time in Korea, China, Japan, etc. (RY Ge, CH Zhou, et al., J. Tra . Chinese . Med ., 3, pp 23-26, 1983; S. Sakamoto, H. Kudo, et al., J. Ethnophamacol ., 23, pp151- 158, 1988; D. Wang, Z. Wang, et al., J. Trad . Chine . Med ., 18, pp 7-11, 1998) antioxidants (T.Okuyama, M. Matsuda, et al., Natural) Medicines , 49, pp 261-265, 1995; T. Kosuge, H. Ishida, M. Ishii, Chem. Pharm . Bull ., 33, pp 1496-1498, 1985). Therefore, the situation mushrooms, Cordyceps sinensis, Ganoderma lucidum mushrooms, which are widely used as food materials, have not been studied.

As described above, the cosmetic composition containing the mushroom extract, Cordyceps sinensis, Ganoderma lucidum mushroom extract of the present invention in the electron donating ability, ABTS radical cation, Superoxide dismutase (SOD) -like activity, xanthine oxidase inhibitory activity and nitrite scavenging activity It has an antioxidant effect and can be usefully used as a cosmetic composition.

In order to achieve the above object, the present invention provides a cosmetic composition containing a mushroom extract, Cordyceps sinensis, Ganoderma lucidum mushroom having an antioxidant effect.

In the case of hot water extract, 10 times distilled water of Sichuan mushroom, Cordyceps sinensis and Ganoderma lucidum was added and reflux-cooled at 85 ° C. for 3 hours to separate supernatant and sediment three times, and ethanol extract was extracted 10 times of 70% ethanol. The amount was added and soaked for 24 hours at room temperature, and extracted three times in the same manner. Each extract was centrifuged, filtered and concentrated and used as a sample for this experiment after freeze-drying. Include.

As a result of measuring the antioxidant effect confirmed by the electron donating ability, ABTS radical cation, Superoxide dismutase (SOD) -like activity, xanthine oxidase inhibitory activity and nitrite scavenging activity of the extract obtained by the extraction method, it can be usefully used as an antioxidant cosmetic composition Can be.

The present invention provides an antioxidant cosmetic composition comprising a situation mushroom, Cordyceps sinensis, Ganoderma lucidum complex extract obtained in the extraction process.

The composition containing the extract of the present invention may be used in various ways, such as cosmetics and face wash for the anti-aging effect of the skin. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

The antioxidant functional cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, emulsion, cream, lotion, pack, foundation, lotion, cosmetic liquid, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

Reference Example  1. Appliances and Appliances

The equipment and apparatus used in this experiment are as follows. UV / vis spectrophotometer (Hitachi 200-10, Japan) and ELISA reader (Bio rad, Co., Japan) were used for the spectroscopic measurements.

Reference Example  2. Experimental reagent

Antioxidant experiments included DPPH (1,1-diphenyl-2-picrylhydrazyl; Sigma chemical Co., USA), ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid); Sigma chemical Co., USA, Pyrogallol (Sigma chemical Co., USA), Xanthine (Sigma chemical Co., USA), Xanthine oxidase (Sigma chemical Co., USA), Gries (Sigma chemical Co. ., USA), BHA (Butylated hydroxyanisole; Sigma chemical Co., USA), Ascorbic acid (Sigma chemical Co., USA) and the like were used.

Experimental Example 1. Determination of the antioxidant effect of the situation mushroom, Cordyceps sinensis, Ganoderma lucidum extract

1-1. Electron donating ability Confirmation test

Electron donating ability (EDA) of S. mushrooms, Cordyceps sinensis and Ganoderma lucidum extracts was performed by modifying the method of Blois (Blois MS, Nature , 26 , pp1199-1120, 1958). 1.0 mL of DPPH (α-α-diphenyl-β-picrylhydrazyl) was added thereto, left to stir for 30 minutes, and the absorbance was measured at 517 nm. The electron donating ability was calculated by Equation 1.

Electron donating ability (%) = (1-reaction zone OD ₅₁₇ / control OD ₅₁₇ ) × 100

 As shown in FIG. 1, under the same conditions, the BHA-treated group of 1,000 ㎍ / ㎖ showed 92.8% of DPPH radical scavenging activity, and the Cordyceps Sinensis extract showed the best electron donating ability among S. mushroom, Cordyceps and Ganoderma lucidum. 90.7% and ethanol extract showed 92.1% of DPPH radical scavenging activity at a concentration of μg / ml. It was confirmed that the ethanol extract showed an electron donating ability similar to the standard BHA.

1-2. ABTS Radical scavenging ability

ABTS radical scavenging experiment is one of the methods for measuring total antioxidant activity (TAA) using the colorless reduced ABTS is oxidized to form a characteristic turquoise ABTS ^{O +} (ABTS radical cation). When the blue-green ABTS ^{O +} reacts with an oxidizable substance, it is reduced to the original colorless ABTS, causing oxidation of the reacted substance, resulting in a decrease in absorbance and measurement of antioxidant capacity (Carola. H. , Carolina.A. And Eduardo.L .: Formation and Decay of the ABTS Derived Radical Cation: A comparison of Different Preparation Procedures.Facultad de Quimica Biologia, Universidad de Santiago Chile, Santiago, Chile; 2002)

Mix 88mL of 7mM ABTS and 140mM K ₂ S ₂ O ₈ and leave it in the dark for 14-16 hours, then mix it with absolute ethanol at a ratio of about 1:88 and adjust the absorbance of the control to 0.7 ± 0.002 at 734 nm. ABTS solution was used. 50 μl of sample solution and 1 mL of ABTS solution were mixed, shaken for 30 seconds, reacted for 2.5 minutes, and absorbance was measured at 734 nm to calculate ABTS radical scavenging ability according to Equation 2.

Radical scavenging activity (%) = (1- reaction OD ₇₃₄ / control OD ₇₃₄ ) × 100

As shown in Figure 2, under the same experimental conditions, 1,000㎍ / ㎖ Ascorbic acid (Vit C) showed 99.8% of ABTS activity and the most active among the situation mushrooms, Cordyceps sinensis, Ganoderma lucidum ethanol extract 98.4% and 95.1% of water extract showed ABTS activity similar to that of Ascorbic acid.

1-3. Superoxidase Dismutase ( Superoxide dismutase ; SOD ) Pseudo-activity  Measure

 SOD-like activity was measured according to the methods of Marklund S and Marklund G (1974). To 0.2 mL of each sample solution, 2.6 mL of Tris-HCl buffer solution (50 mM tris + 10 mM EDTA, pH 8.5) and 0.2 mL of 7.2 mM pyrogallol were added and reacted at 25 ° C. for 10 minutes, and then 0.1 mL of 1M HCl was added to stop the reaction. The amount of heavy oxidized pyrogallol was measured at 420 nm to show SOD-like activity according to Equation 3.

Superoxide dismutase (SOD) -like activity (%) = {(control OD ₄₂₀ -sample OD ₄₂₀ ) / control OD ₄₂₀ } × 100

As shown in Figure 3, under the same experimental conditions, 1,000㎍ / ㎖ Ascorbic acid (Vit C) showed a 99.3% SOD-like activity, the most active of the situation mushroom, Cordyceps sinensis, Ganoderma lucidum ethanol extract SOD-like activity of 102.8% in water and 92.5% in water extract showed similar activity to Ascorbic acid.

1-4. Xanthine oxidase inhibition  Active measurement

To 0.6 mL of 0.1M phosphate buffer (pH 7.5), add 0.1 mL of Sichuan mushroom, Cordyceps sinensis and Ganoderma lucidum extract and 0.2 mL of 1 mM xanthine. 0.2 mL of 0.2 Unit / mL xanthine oxidase was added and reacted at 37 ° C. for 5 minutes, and then 20% TCA was added to terminate the reaction. After centrifugation to remove the protein, the uric acid produced in the reaction solution was measured by absorbance at 292nm. Xanthine oxidase inhibitory activity was calculated using the following equation (4) which is the absorbance decrease rate of the addition and no addition of the sample solution.

Xanthine oxidase inhibition rate (%) = {(control OD ₂₉₂ -sample OD ₂₉₂ ) / control OD ₂₉₂ } × 100

As shown in Fig. 4, xanthine oxidase inhibitory activity was the most active among the situation mushrooms, Cordyceps sinensis and Ganoderma lucidum in the case of ethanol extracts of 1,000.mu.g / ml and 100% xanthine oxidase inhibition in the water extract of 1,000.mu.g. Activity was shown. Under the same conditions, 1,000 μg / ml of BHA showed an effect of 83.3%, and thus the situational mushroom extract showed higher inhibitory activity than the xanthine oxidase inhibitory activity of BHA.

1-5. nitrite Scatters  Measure

Nitrite scavenging activity was measured by Gray and Dugan (J. Grayand, Jr. LR Dugan, J. Food . Sci., 40, pp981-985, 1975). 1 mL of sample and 1 mL of 1 mM NaNO ₂ were calibrated to pH 1.2 with 0.1 N HCl to adjust the volume of the reaction solution to 10 mL. After the reaction solution was reacted at 37 ° C. for 1 hour, 1 mL of each reaction solution was taken and the reaction was stopped with 5 mL of 2% acetic acid solution, and 0.4 mL of Gries reagent was added thereto. After stirring, the mixture was left at room temperature for 15 minutes, and the absorbance was measured at 520 nm. The amount of nitrite was measured by Equation 5. The control group was measured by adding distilled water instead of the Gries reagent, and the nitrite scavenging ability was expressed as a decrease in the absorbance of the sample sphere relative to the control.

Nitrite scavenging activity (%) = {(control OD ₅₂₀ -sample OD ₅₂₀ ) / control OD ₅₂₀ } × 100

As shown in FIG. 5, 1,000 μg / ml of the positive control BHA showed 62.8% nitrite scavenging ability. Under the same conditions, the most active among the situation mushrooms, Cordyceps sinensis and Ganoderma lucidum mushrooms showed the nitrite scavenging ability of 61.3% and the ethanol extract of 70.5%. The nitrite scavenging ability was superior to that of the BHA standard.

The extracts of S. mushrooms, Cordyceps sinensis and Ganoderma lucidum mushrooms had better antioxidant activities than those of other extracts and had similar antioxidant properties to BHA.

1-Situation mushroom, Cordyceps sinensis, Ganoderma lucidum water extract and ethanol extract is a graph showing the measurement of the electron donating ability.

Figure 2-Situation mushroom, Cordyceps sinensis, Ganoderma lucidum water extract and ethanol extract is a graph showing the measurement of ABTS radical cation activity.

Figure 3-Situation mushroom, Cordyceps sinensis, Ganoderma lucidum water extract and ethanol extract Superoxide dismutase (SOD) similar activity was measured and shown the graph.

Figure 4-Situation mushroom, Cordyceps sinensis, Ganoderma lucidum water extract and ethanol extract Xanthine oxidase inhibitory activity measured.

Figure 5-Situation of mushrooms, Cordyceps sinensis, Ganoderma lucidum water extract and ethanol extract nitrite scavenging ability.

Claims (2)

Stomach mushroom, Cordyceps sinensis, Ganoderma lucidum hot water extract and ethanol extract having an antioxidant effect. Situation mushroom, Cordyceps sinensis, Ganoderma lucidum extract mixture ratio (50: 10: 1) (w / w / w) which has antioxidant effect

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