KR20100101897A - Eco-friendly agriculture matter of function showing biocontrol and biofertilizing activites - Google Patents

Eco-friendly agriculture matter of function showing biocontrol and biofertilizing activites Download PDF

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KR20100101897A
KR20100101897A KR1020090020330A KR20090020330A KR20100101897A KR 20100101897 A KR20100101897 A KR 20100101897A KR 1020090020330 A KR1020090020330 A KR 1020090020330A KR 20090020330 A KR20090020330 A KR 20090020330A KR 20100101897 A KR20100101897 A KR 20100101897A
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이중복
권기석
최충식
조민섭
전춘표
김윤회
최성연
조종일
김건우
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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Abstract

PURPOSE: An environmentally-friendly microorganism fertilizer using a novel Bacillus sp. is provided to control growth promotion of rice plants and to induce nutrition change of rice plants. CONSTITUTION: An environmentally-friendly agricultural agent contains Bacillus sp. AM-651[Bacillus sp. AM-651] culture medium(deposit number: KCTC 11305BP) which is effective in biocontrol and as a biofertilizer. Bacillus sp. AM-651 culture medium (deposit number: KCTC 11305BP) promotes growth rice plants. A Bacillus sp. AM-651(deposit number: KCTC 11305BP) converts insoluble phosphorus to soluble phosphorus.

Description

생물방제 및 생물 비료 효과를 가지는 기능성 친환경농업제제 {Eco-friendly agriculture matter of Function showing biocontrol and Biofertilizing activites}Eco-friendly agriculture matter of Function showing biocontrol and Biofertilizing activites

본 발명에서는, 생물 비료 효과와 생물방제 효과를 가지는 친환경농업제제인 첨가제를 개발하고자 하였다. In the present invention, it was intended to develop an additive which is an environmentally friendly agricultural agent having a biofertilizer effect and a biocontrol effect.

　즉, 본 발명의 주된 목적은 벼 재배에 있어 생산량 증대와 영양성분의 변화를 주는 친환경농업제제인 미생물 첨가제를 제공한다. That is, the main object of the present invention is to provide a microbial additive, which is an environmentally friendly agricultural agent that increases production and changes in nutrients in rice cultivation.

　본 발명의 또 다른 목적은 상기의 균주를 이용하는 토양에 고정된 난용성 인을 효과적으로 가용화시켜 인산질 비료의 절감에 효능을 주는 친환경농업제제인 미생물 첨가제를 제공하는데 있다. Still another object of the present invention is to provide a microbial additive which is an environmentally friendly agricultural agent that effectively solubilizes poorly soluble phosphorus immobilized on soil using the above strains, thereby reducing phosphate fertilizer.

　또한 균주의 배양액을 비료첨가제로 처리하였을 때 벼의 생육촉진제로서의 활용 가능성을 제공하는데 있다. In addition, when the culture medium of the strain is treated with a fertilizer additive to provide the possibility of utilization as a growth promoter of rice.

본 발명은 분리균주 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367)배양액을 이용하여 벼 재배에 있어 친환경적으 로 농사를 지으며 미곡 증진 효과 관한 것이다. 더욱 상세하게는 벼의 전 생육기간에 신규 미생물 균주인 바실러스 속 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367)를 이용하여 생물 비료 효과와 화학 농약을 대체할 생물농약효과를 줄 수 있는 친환경농자제에 관한 것이다.The present invention is a genus of isolated strain Bacillus AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) It is related to the rice grain enhancement effect in the environmentally friendly farming in rice cultivation using the culture solution. More specifically, the genus Bacillus, a novel microbial strain for the entire growth period of rice. AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) relates to an eco-friendly agricultural agent that can give a biological fertilizer effect and a biological pesticide effect to replace the chemical pesticide.

　 현재 친환경 농업에 관한 연구를 보면 주로 생물 농약을 이용한 활성 비교와 생물비료개발을 위한 균주 개발을 분리하여 연구를 추진해오고 있었으며, 동시적으로 생물농약과 생물비료를 동시에 효과를 줄수 있는 기능성 미생물 제제에 관한 연구는 미미한 상태이다 {참조 정 등, J. Kor . Soc . Appl . Biol . Chem.. 50. 2007; 정 등 한국생명과학회지 17, 2007; Sessitsch 등, Can . J. Microbiol . 50. 2004}Currently, research on eco-friendly agriculture has been conducted by separating the activity comparison using biopesticides and the development of strains for the development of biofertilizers, and at the same time, on functional microbial preparations that can simultaneously effect biopesticides and biofertilizers. There is little research on this. {See Jung et al . , J. Kor . Soc . Appl . Biol . Chem. . 50. 2007; Jung et al. Korean Journal of Life Science 17, 2007; Sessitsch et al., Can . J. Microbiol . 50. 2004}

　정밀화학의 발달로 화학 농약 방제 및 화학비료에 대해 여러 가지들이 사용되고 있으나, 최근 환경파괴와 농약사용 농산물에 대한 소비자들의 기피, 저항성 균주의 증가로 친환경농업제제의 개발이 불가피한 실정이다. Due to the development of fine chemistry, various kinds of chemical pesticide control and chemical fertilizers are used, but the development of eco-friendly agricultural products is inevitable due to the recent environmental destruction, the evasion of consumers and the increase of resistant strains.

따라서 본 발명은 토양내에 존재하고 있는 난용성인을 이용하여 가용성 인으로의 전환을 통해 인산질비료의 사용을 줄이며, 미생물이차대사산물을 이용하여 생장 촉진 및 쌀생산에 있어 생산율을 높이며 생물방제를 통해 친환경적으로 재배가 가능하도록 하는데 목적이 있다. Therefore, the present invention reduces the use of phosphate fertilizer by converting into soluble phosphorus using poorly soluble phosphorus present in the soil, increasing the production rate in promoting growth and rice production by using microbial secondary metabolites and eco-friendly through biocontrol. The purpose is to enable cultivation.

본 발명은 토양으로부터 분리한 바실러스 종 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367) 균주 배양액을 이용하여 토양에 고정된 난용성 인산염을 가용화하여 식물이 쉽게 사용할 수 있도록 도움을 주며, 배양액으로부터 생물 비료활성 (식물성장촉진호르몬 (IAA) 유사물질)과 생물방제활성(식물병원균 저해능)을 가지는 친환경농업제제 개발에 관한 것이다.Bacillus sp. AM-651 isolated from soil (Accession No .: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) Soluble soluble phosphate immobilized in soil using strain culture solution to help the plant to be used easily, and from the culture solution to biological fertilizer activity (plant growth hormone (IAA) analogue) The present invention relates to the development of eco-friendly agricultural preparations having biological control activity (phytopathogenic activity).

본 발명에 따른 바실러스 속 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651)배양액을 이용한다면, 쌀 농사에 있어 화학 농약 및 화학 비료의 대체할 생물농약 뿐만 아니라 미곡 증진제로의 활용이 가능할 것으로 사료된다. Bacillus genus according to the invention AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. If AM-651) is used, it may be possible to use rice as a rice enhancer as well as a biopest to replace chemical pesticides and chemical fertilizers.

본 발명은 바실러스 속 균주의 배양액을 이용하여 생물방제와 생물 비료효과를 보이는 친환경농업제제의 기초를 제공한다. 본 발명에 따른 바실러스 속 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651)배양액을 이용한다면, 쌀 농사에 있어 화학 농약 및 화학 비료의 대체할 생물농약 뿐만 아니라 미곡 증진제로의 활용이 가능할 것으로 사료된다. The present invention provides a basis for environmentally friendly agricultural preparations that exhibit biocontrol and bio fertilizer effects using cultures of the strains of the genus Bacillus. Bacillus genus according to the invention AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. If AM-651) is used, it may be possible to use rice as a rice enhancer as well as a biopest to replace chemical pesticides and chemical fertilizers.

　본 발명에서는 상기의 목적을 달성하기 위하여, 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367)를 이용한 생물비료 및 생물방제효과를 주는 친환경농업제제 미생물첨가제를 제공한다. In the present invention, in order to achieve the above object, the genus Bacillus AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) provides an eco-friendly agricultural preparation microbial additives having a biological fertilizer and a biocontrol effect.

　본 발명의 미생물첨가제는 그 배양액을 유효성분으로 함유하는 생물비료 및 생물방제효과를 주는 친환경농업제제의 기초를 제공한다. The microbial additive of the present invention provides a basis for an ecological fertilizer and an environmentally friendly agricultural formulation that provides a biocontrol effect containing the culture as an active ingredient.

　이하 실시예를 통하여 본 발명을 보다 상세하게 설명하고자 한다. Through the following examples will be described in more detail the present invention.

실시예 1 :　 Bacillus sp . AM - 651균주 배양액의 상세히 설명한다. Example 1: Bacillus sp . The AM - 651 strain culture medium will be described in detail .

Bacillus sp. AM-651를 전배양하여 멸균 배지에 (5g Glucose, 2g KH2PO4 , 1g (NH4)2SO4, 7g K2HPO4, 0.1g MgSO47H2O, 0.1g Yeast Extract, 1L 증류수) 접종하여 3일간 180rpm 30℃로 배양하여 제제화의 원재로 사용하였다. Bacillus sp. Pre-incubate AM-651 in sterile medium (5g Glucose, 2g KH 2 PO 4 , 1g (NH 4 ) 2 SO 4 , 7g K 2 HPO 4 , 0.1g MgSO 4 7H 2 O, 0.1g Yeast Extract, 1L distilled water ) Was inoculated and incubated at 180 rpm 30 ° C. for 3 days to be used as a raw material for formulation.

　

실시예Example 2 : 균주의 가용성 인산  2: soluble phosphoric acid of strain 가용화량Solubilization 측정. Measure.

　난용성 인산의 가용화 측정을 위하여 NBRIP agar Plate (0.1g Glucose, 0.001g (NH4)2SO4, 0.0025g MgSO47H2O, 0.05g MgCl26H2O, 0.002g KCl, 0.05g Ca3(PO4)2 를 멸균하여 AM-651균주를 접종하여 확인 하였으며, 배양액 중의 인산가용화측정방법은 NBRIP agar Plate에서 agar를 제외한 액상에서 균주를 접종하여 2day 간격으로 배양액을 채취하여 인산가용량을 측정하였다. 대조구는 처리구와 동일한 배지에 뷴리 균주를 사멸 후 접종하여 측정하였다. 측정방법은 배양액을 13,000rpm 에서 10분간 원심분리를 한 후 0.45um의 필터에 여과 한 후 몰리브덴산법으로 하여 측정하였다. 측정결과 배양액중 난용성 3인산 칼륨은 량은 대조구에서는 1일, 3일, 5일동안 0~1.8mg/l로 변화가 없었으며, AM-651균주는 1일, 3일, 5일 동안 85, 347, 380mg/l으로 매우 높게 인산 사용화량을 증가 시키는 효과를 보였다. NBRIP agar Plate (0.1g Glucose, 0.001g (NH 4 ) 2 SO 4 , 0.0025g MgSO 4 7H 2 O, 0.05g MgCl 2 6H2O, 0.002g KCl, 0.05g Ca 3 (PO 4 ) 2 was sterilized and inoculated with strain AM-651, and the method of measuring phosphate solubilization in the culture solution was measured by inoculating the strain in the liquid phase except for agar in the NBRIP agar plate and collecting the culture solution at intervals of 2 days. The strain was inoculated after killing strains inoculated on the same medium as the treatment.Measuring method was centrifuged at 13,000rpm for 10 minutes, filtered through 0.45um filter, and measured by molybdate method. The amount of poorly soluble potassium triphosphate did not change from 0 to 1.8 mg / l for 1, 3 and 5 days in the control, and the AM-651 strain was 85, 347 and 380 mg for 1, 3 and 5 days. / l showed a very high effect of increasing the amount of phosphoric acid used.

실시예Example 3 :  3: 바실러스Bacillus AMAM -651 균주의 Of -651 strain indoleindole -3--3- aceticacetic acidacid ( ( IAAIAA )의 생산량 측정Yield measurement

　indole-3-acetic acid (IAA)는 식물 성장 호르몬으로 식물성장을 촉진 시켜주는 물질이다. 이러한 식물생장 호르몬인 IAA의 생성능은 배양액을 13,000rpm 에서 10분간 원심분리를 한 후 0.45u의 필터에 여과 한 후 Salkowsy reagent와 반응시켜 분홍생을로 변환되는 것을 IAA 생성능으로 결정하여 흡광광도계535nm에서 측정하였다. IAA측정 시 농도는 Trytophan 미처리시 5.07ppm, 0.1% Trytophan 처리시 41.21ppm으로 증가하여 나타났으며, 배양액을 1/500~1/1,000배 처리하였을 경우 성장율이 가장 좋았다. Indole-3-acetic acid (IAA) is a plant growth hormone that stimulates plant growth. The production capacity of IAA, a plant growth hormone, was centrifuged at 13,000 rpm for 10 minutes, filtered through 0.45 u of filter, and then reacted with Salkowsy reagent to determine pink I. Measured. When IAA was measured, the concentration was increased to 5.07ppm without Trytophan and 41.21ppm with 0.1% Trytophan treatment, and the growth rate was the best when 1/500 ~ 1 / 1,000 times of culture was treated.

실시예Example 4 :  4 : 바실러스Bacillus AMAM -651 균주의 생물방제 효과 측정Determination of Biocontrol Effects of -651 Strains

벼잎무늬마름병에 대한 생물방제 효과 측정에 있어 19.98mm 우수한 항균능을 보이는 것으로 조사되어졌다. It was found that the antimicrobial activity of 19.98mm was excellent in measuring biocontrol effects on rice leaf blight.

실시예Example 5 :  5: 바실러스Bacillus 속 genus AMAM -651 배양액이 벼 생육에 미치는 영향Effect of C-651 Culture on Rice Growth

　바실러스 속 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651)의 배양액을 벼의 생육에 미치는 영향을 조사한 결과 초장 길이로 조사한 결과 1/1000으로 희석 후 관주처리하였을 때 벼의 생육이 가장 높았으며, 논처리 하였을 때도 1/1000현탁하여 처리하였으며, 미곡의 생산량과 영양성분의 변화가 보인것으로 나타났다. Bacillus genus AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. As a result of investigating the effect of the culture medium of AM-651) on rice growth, the highest growth of rice was obtained when dilution to 1/1000 and then irrigation treatment. In addition, changes in the yield and nutritional content of rice were shown.

　 대조구Control 실험구Experiment 열량calorie 353.7Kcal353.7 Kcal 356.5Kcal356.5Kcal 탄수화물carbohydrate 81.4g/100g81.4g / 100g 79.9g/100g79.9g / 100g 식이섬유Dietary Fiber 1.5g/100g1.5g / 100g 3.5g/100g3.5g / 100g 당류(유리당)Sugars (free sugar) 0.9g/100g0.9g / 100g 2.3g/100g2.3g / 100g 단백질protein 5.1g/100g5.1g / 100g 5.5g/100g5.5g / 100g 지방Fat 1.2g/100g1.2g / 100g 2.4g/100g2.4g / 100g 포화지방Saturated fat 0.4g/100g0.4g / 100g 1.1g/100g1.1g / 100g 트랜스지방trans fat 0g/100g0g / 100g 0g/100g0g / 100g 콜레스테롤cholesterol 0mg/100g0mg / 100g 0mg/100g0mg / 100g 나트륨salt 0.5mg/100g0.5mg / 100g 0.5mg/100g0.5mg / 100g

도 1은 바실러스 속 AM-651　(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367)의 고체배양에서의 인산칼슘에 대한 가용화능을 나타낸 것이다. 1 is a genus of Bacillus AM-651 (Accession Number: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) shows the solubilization capacity for calcium phosphate in solid culture.

도 2는 액체배양 후 가용화능을 몰리브덴산화법으로 측정한것이다.2 is a measurement of the solubilization capacity after the liquid culture by molybdenum oxidation method.

도 3은 AM-651 벼잎집무늬마름병 KACC40106의 생물방제활성을 측정한 것이다.Figure 3 measures the biocontrol activity of AM-651 rice leaf pattern blight KACC40106.

도 4은 Indole-3-acetic acid (IAA)의 생산량 측정을 측정한 것이다.Figure 4 measures the measurement of the production of Indole-3-acetic acid (IAA).

도 4는 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651; 출원번호 10-2008-0037367)처리 벼 모의 성장 결과이다.4 is genus Bacillus AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651; Application No. 10-2008-0037367) is the result of growing simulated rice.

도 5는 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus asp. AM-651; 출원번호 10-2008-0037367)처리시 벼의 성장 사진이다.5 is genus Bacillus AM-651 (Accession No .: KCTC 11305BP, Bacillus a sp. AM-651; Application number 10-2008-0037367) is a photograph of the growth of rice during treatment.

Claims (7)

　 벼 농사에 생물방제 및 생물비료로 효과적인 미생물 바실러스 속 AM-651배양액 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651)의 친환경농업제제 A genus of microbial Bacillus effective as biocontrol and bio fertilizer for rice farming AM-651 culture solution (Accession No .: KCTC 11305BP, Bacillus sp. AM-651 eco-friendly agricultural product 제1항에 있어서, 벼농사시 모자리에 생육을 촉진하는 미생물 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651) 배양액.The microorganism Bacillus genus according to claim 1, which promotes growth of moieties during rice farming. AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) culture. 　제1항에 있어서, 미생물 바실러스 속 AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651)배양액을 토양에 처리 하였을 경우 토양에 불용성 인을 가용성 인으로 전환하는 방법The method of claim 1, wherein the microorganism Bacillus genus AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) Method of Converting Insoluble Phosphorus into Soluble Phosphorus in Soil when Culture Solution is Treated in Soil 제2항에 있어서, 미생물 바실러스 sp. AM-651 (기탁번호 : KCTC 11305BP, Bacillus sp. AM-651) 균주 또는 배양액이 식물생장 촉진하는 미생물 비료The method of claim 2, wherein the microorganism Bacillus sp. AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) Microbial Fertilizers Promote Plant Growth by Strains or Cultures 제1항에 있어서, 벼농사시 벼의 식물 병원균을 방제하는 미생물 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651) 배양액The microorganism Bacillus genus according to claim 1, which controls plant pathogens of rice during rice farming. AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) medium 제1항에 있어서, 벼농사시 벼의 성장을 촉진하는 미생물 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651) 배양액The microorganism Bacillus genus according to claim 1, which promotes the growth of rice during rice farming. AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) medium 제1항에 있어서, 벼 재배후 쌀의 영양성분의 변화를 주는 미생물 바실러스 속 AM-651(기탁번호 : KCTC 11305BP, Bacillus sp. AM-651) 배양액The microorganism Bacillus genus according to claim 1, which changes the nutritional content of rice after rice cultivation AM-651 (Accession No .: KCTC 11305BP, Bacillus sp. AM-651) medium
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101487879B1 (en) * 2012-11-27 2015-02-03 한국생명공학연구원 Bacillus amyloliquefaciens Having an Antagonistic Effect for Ganoderma Boninense Causing Basal Srem Rot Disease in Oil Palm

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101487879B1 (en) * 2012-11-27 2015-02-03 한국생명공학연구원 Bacillus amyloliquefaciens Having an Antagonistic Effect for Ganoderma Boninense Causing Basal Srem Rot Disease in Oil Palm

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