KR20100086728A - Cosmetic composition for skin whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin whitening is provided to treat or prevent dyspigmentation and hyperpigmentation. CONSTITUTION: A cosmetic composition for skin whitening contains 0.001-10 weight% of Saururus chinensis Baill extract, 0.001-10 weight% of poly gamma glutamic acid and 0.1-80 weight% of glacial water as active ingredient. The glacial water is from Hunza of Himalaya or Vilcabamba of Ecuador.

Description

Cosmetic composition for skin whitening {Cosmetic composition for skin whitening}

The present invention relates to a cosmetic composition for skin whitening that improves or prevents skin pigment abnormalities and skin pigment overdeposition and improves dull and rough skin texture to implement a whitening effect. More specifically, the present invention relates to a cosmetic composition for skin whitening using tritical extract, polygamma glutamic acid and glacial water as an active ingredient to improve rough skin surface and brighten the skin tone as a whole.

Human skin color depends on the amount of melanin, carotene and hemoglobin, of which melanin is the most decisive factor. Melanin is synthesized in melanocytes, pigment cells present in the epidermis, and transferred to the surrounding keratinocytes to show human skin color. Overproduction of melanin forms one of women's skin problems, blemishes and freckles, and is also closely related to skin cancer.The formation of melanin is associated with physiological and environmental factors such as genetic factors, hormone secretion, stress and ultraviolet rays. Factors are known to have an effect.

An enzyme that plays a decisive role in melanin synthesis is tyrosinase. Tyrosinase causes tyrosine to be a waveguide, and the waveguide is made into dopaquinone. The next steps of these two reactions are known to be possible by non-enzymatic reactions, and tyrosinase alone is known to produce melanin. Recently, a new theory of melanin formation following the expression of signal transduction agents in skin cells has been proposed. That is, when the skin is exposed to an external environment such as ultraviolet rays and is stressed, interleukin-alpha, which causes inflammation, is expressed from keratinocytes, and this interleukin-alpha promotes keratinocyte endocelin expression. This series of processes increases the formation of melanocytes and activates melanocytes so that tyrosine is melanin formed by tyrosinase in the melanosomes.

 Most of the whitening cosmetic ingredients such as kojic acid and arbutin have been proven to inhibit tyrosinase, but they have low effect in experiments similar to the actual bio-levels. Due to its stability and insufficient whitening effect, its use is limited.

In view of the above-described inhibition of melanin production, it is difficult to fully anticipate its whitening effect, and researches to improve pigment problems such as skin color, blemish and blemishes are being actively conducted by managing and improving the melanin produced. In other words, when the keratinocytes are produced and eliminated abnormally by drying the skin and the keratinocytes are not differentiated normally, the skin surface becomes rough and the skin tone becomes dull overall. In order to solve this problem, the skin tone can be smoothly and brightly improved by creating a skin environment in which proper moisture can be supplied to the stratum corneum, and thus a whitening effect can be obtained.

The present inventors have studied to find a cosmetic composition showing an excellent whitening effect, the extract of three hundred seconds that can suppress the production of melanin by external stress, polygamma glutamic acid showing excellent skin moisturizing effect by promoting the production of hyaluronic acid of dermal fibroblasts, and When moisturizing effect of mineral was used together with glacier water with keratinizing effect, it showed excellent whitening effect.

Accordingly, an object of the present invention is to contain the extract of three hundred seconds, poly-gamma glutamic acid and glacial water as an active ingredient to inhibit the formation of skin melanin cells and to prevent or improve the skin pigmentation phenomenon such as blemishes, freckles, blemishes to clean the skin tone as a whole It is to provide a cosmetic composition for skin whitening that can obtain the effect of improving the dullness.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening containing three hundred seconds extract, poly gamma glutamic acid and glacial water as an active ingredient.

The cosmetic composition provided by the present invention reduces the keratin by moisturizing effect by the three hundred seconds extract that can inhibit melanin production by external stress, hyaluronic acid production of dermal fibroblasts, polygamma glutamic acid showing excellent skin moisturizing effect, and minerals By using effective glacier water, it is possible to reduce excessive angular mass and improve dull and rough skin tone by blemishes, blemishes, and skin hydration through melanin production. Therefore, the cosmetic composition for skin whitening according to the present invention shows an excellent whitening effect than the existing whitening cosmetics and is very useful as a whitening agent.

The present invention provides a cosmetic composition for skin whitening containing three hundred sec extract, poly gamma glutamic acid and glacial water as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The three hundred seconds (Saururus chinensis Baill) used as an active ingredient in the present invention is a perennial herb of the dicotyledonous plant, which is called three hundred seconds, which is called three hundred seconds. It is known to have a remarkable effect on the prevention and treatment of various adult diseases such as constipation, diabetes, liver disease, cancer, high blood pressure, heart disease, gynecological disease and kidney disease. Fravonoids such as quercetin, quercitzin, sisotuercitri and rucin, which are identified as active substances of three hundred seconds, clear the blood beyond ginkgo leaf prabonnes and provide a lot of blood evenly throughout each tissue of the whole body. Peripheral arterial protective action, water-soluble tannins have the ability to prevent DNA (gene) mutations have been shown to have a high carcinogenic activity. In addition, the extract may be used as a whitening cosmetic composition using the activity inhibitory action of tyrosinase, a melanin-producing enzyme (Korean Patent Application Publication No. 2002-0035656).

The three hundred sec extract according to the present invention may be prepared by an extraction method common in the art, and the method is not particularly limited. For example, 1 kg of 300 seconds is added to 5 L of water, an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, or methylene chloride) or a mixed solvent thereof, and the mixture is extracted under reflux three times and then immersed at 15 ° C. for 1 day. . Thereafter, the residue and the filtrate are separated by filter cloth filtration and centrifugation, and the extract obtained by concentrating the separated filtrate under reduced pressure is suspended in 1 L of water, and then extracted five times with 1 L of methylene chloride. The methylene chloride layer obtained therefrom was concentrated under reduced pressure to obtain 50 g of three hundred sec extracts.

Poly-gamma glutamic acid (Poly-γ-glutamic Acid, γ-PGA) used as an active ingredient in the present invention is a sticky mucus material contained in Cheonggukjang, a traditional fermented food that has been eaten for more than a thousand years. It is an anionic amino acid polymer in which the α-amino group of glutamic acid is amide-bonded. It is known that the skin moisturizes and anti-wrinkles by inhibiting hyaluronic acid deactivating enzyme activity. The polygamma glutamic acid is produced from strains of the genus Bacillus isolated from Korea's traditional soybean fermented food Cheonggukjang, Japanese traditional soybean fermented food natto or Nepalese traditional soybean fermented food kinema. In the present invention, using the salt-resistant strain Bacillus subtilis var. Chungkookjang to produce a high molecular weight poly gamma glutamic acid to prepare a poly gamma glutamic acid, having a molecular weight of 2.0 × 10 ⁴ ~ 1.0 × 10 ⁷ Da The use of polygammaglutamic acid is preferred for skin moisturizing and anti-wrinkle efficacy.

The glacial waters used as an active ingredient in the present invention have a unique structure in which colloidal silica is formed in the nano-sized water and has a low surface tension, so that the active ingredient is easily absorbed by the human body and skin. Let's do it. In addition, glacial water is a calcium, sodium, magnesium, potassium, zinc and iron that plays a role in osmotic pressure, acid and alkali equilibrium, wound healing and metabolic functions in the body, and plays an important role in oxygen transfer and cellular respiration. It is known to be rich in essential ionized minerals required by the human body. It is preferable to use glacial water produced in the Himalayan hunja, Ecuador bilcabamba, or the Abkhazia region of the Caucasus for the exfoliation effect and skin moisturizing.

The cosmetic composition for skin whitening according to the present invention is characterized in that it contains 0.001 to 10% by weight, 0.001 to 10% by weight of polygamma glutamic acid and 0.1 to 80% by weight of glacial water based on the total weight of the composition.

When the content of each of the three hundred sec extract and poly gamma glutamic acid is less than 0.001% by weight relative to the total weight of the composition, it is difficult to expect the effect of inhibiting skin moisturizing and melanin production, and when the content exceeds 10.0% by weight, the effect is no longer increased significantly. The efficiency of the raw material is reduced, and the feeling of use of the formulation is greatly deteriorated, which lowers the value as a cosmetic. In addition, when the glacial water is less than 0.1% by weight relative to the total weight of the composition, the moisturizing effect and keratin reduction effect is insignificant, and when the glacial water exceeds 80% by weight, the effect is no longer increased significantly, and thus the efficiency of the raw material is reduced. .

White Plus Renew Complex, which contains a mixture of three hundred sec extract, polygamma glutamic acid and glacial water according to the present invention, is a combination based on scientific basic data on the skin. You can actually implement it.

The cosmetic composition of the present invention is not particularly limited in its formulation, and may be, for example, a cosmetic composition having a formulation of a flexible cosmetic water, nourishing cosmetic water, massage cream, nourishing cream, pack, gel or skin adhesive type cosmetic.

Hereinafter, although an Example and a test example are given and this invention is demonstrated more concretely, the scope of the present invention is not limited to the following Example, a test example, etc.

Preparation Example 1 Preparation of Three 300 Seconds Extract

1 kg of 300 seconds was placed in 5 L of 70% ethanol, and refluxed three times, followed by immersion at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure, suspended in 1 L of water, and then extracted 5 times with 1 L of methylene chloride. The methylene chloride layer thus obtained was concentrated under reduced pressure to obtain 50 g of three hundred sec extracts.

Test Example 1 Melanin Production Inhibition Test

HaCaT and keratinocytes were laid on a 24-well plate to 1.5 × 10 ⁵ cells / well, and then left in a cell incubator for 24 hours. Then, after diluting the 300 seconds extract obtained in Preparation Example 1 to a concentration of 1ppm and put the medium containing and incubated for 1 day, the addition of pollution and tobacco fumes of the pollutant and left in the cell culture for 2 more days Afterwards, the medium was harvested.

Melan-a cells were plated on a 24-well plate to be 3 × 10 ⁴ cells / well, left in the cell incubator for cells to adhere to the plate for 24 hours, and then the keratinocyte medium and RPMI1640 (w / serum and 200 nM PMA) were added at a ratio of 3: 7. In this way, the experimental samples were treated once every three days for six days. The cells are then dissolved in a cell lysis buffer, and the supernatant is separated by centrifugation to quantify the protein. Melanin is measured by absorbance at 405 nm by adding 1N NaOH. It is shown in Table 1 below in terms of the amount of melanin.

Badge condition Melanin
(Melanin amount / protein amount) No treatment 1.0 250ppm Tobacco Treatment 1.74 200 ppm of car fumes 1.48 250 ppm of tobacco after 1ppm treatment of 300 sec extract 0.37 200 ppm of soot after treatment with 1ppm of 300 seconds extract 0.61

As can be seen from Table 1, it was confirmed that the extract of the three hundred seconds, the active ingredient of the present invention can effectively reduce the amount of melanin increased by external stress, such as tobacco or automobile smoke. Therefore, the cosmetic composition for skin whitening of the present invention was able to obtain excellent whitening effects by relieving melanin production and relieving blemishes and blemishes by improving skin pigment overdeposition and improving skin tone.

Test Example 2 Hyaluronic Acid Production Promoting Test

Dermal fibroblasts were placed on a 12-well plate to 1 × 10 ⁵ cells / well, and then washed twice after incubation for 24 hours, and treated with polygamma glutamic acid (Bio Leaders) for each concentration. After 48 hours, the cultures were collected and treated at 2 concentrations in a dot blot. Antibody to HABP conjugated with HRP (Horse radish peroxidase) after 1 hour treatment of hyaluronic acid binding protein (HABP) that specifically binds hyaluronic acid to a concentration of 1: 100 , HCJ-242) was treated at a concentration of 1: 500 for 1 hour. After washing twice with TBST buffer, it was detected and quantified by the LAS3000 Chemiluminescence machine, and the results are shown in Table 2 below.

Badge condition Hyaluronic Acid Production Rate
(% Of untreated) No treatment (0ppm) 100% Polygamma Glutamic Acid (50ppm) 168.75% Polygamma Glutamic Acid (100ppm) 191.63%

As can be seen in Table 2, the hyaluronic acid production rate of the dermal fibroblasts was increased as the concentration of the polygamma glutamic acid as the active ingredient of the present invention increases. Therefore, the cosmetic composition for skin whitening of the present invention may contain polygamma glutamic acid to increase hyaluronic acid production and provide excellent skin moisturizing effect, and the skin tone may be smoothly and brightly improved through maintaining the skin moisturizing to show an excellent whitening effect. .

Example 1 and Comparative Examples 1 to 7

In order to measure the whitening effect and angular mass change on the human skin, Examples 1 and Comparative Examples 1 to 7 were prepared by the composition of Table 3 (unit: wt%).

Using the three hundred seconds extract obtained in Preparation Example 1 and the polygamma glutamic acid of Bio Leaders Inc., was collected from Ultarpic of height 7388m and subjected to a process such as filtering Himalayan glacier water from Interlis.

Ingredient Name Example
One Comparative example One 2 3 4 5 6 7 1. Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 2. Glycerin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3. Butylene Glycol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 4. Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 5. Three hundred sec extract 1.0 - 1.0 - - 1.0 1.0 - 6. Polygamma glutamic acid 0.5 - - 0.5 - 0.5 - 0.5 7. Himalayan Glacial Water 1.0 - - - 1.0 - 1.0 1.0 8. Caprylic Capric Triglycerides 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 9. Hydrogenated Polydecene 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 10. Cetearylglucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 11.Sorbitan Stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 12. Cetearyl Alcohol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 13. Preservative Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 14. Incense Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 15. Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1

<The manufacturing method of Example 1 and Comparative Examples 1-7>

1) The components 1-7 were mixed uniformly, melt | dissolved at 70 degreeC, and it was set as the water part.

2) The components 8-13 were melt | dissolved at 70 degreeC, and it was set as the oil part.

3) The oil part of 2) was added to the water part of 1) and stirred with a homomixer (Tokushu Kika, Japan) to emulsify first.

4) Components 14 and 15 were added to 3) to increase.

5) After removing the bubbles, the mixture was cooled at room temperature.

Test Example 3 Whitening Effect Test on Human Skin

After attaching an opaque tape with six holes of 1.5cm in diameter to the upper forearm of 20 subjects, about 1.5 ~ 2 times the UVB of each subject's minimum erythema (Minimal Erythema Dose). The blackening of the skin was induced by irradiating and the test materials were applied and two months later, the contrast of the skin was measured using a color difference meter. Each test substance was applied to the skin external preparation of Example 1 and Comparative Examples 1 to 7 twice daily in the morning and evening.

The determination of the effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L, a, and b colorimeters are used to display the color. In the present invention, the "L" value representing the light and dark of the skin was obtained and analyzed (the unburned Korean skin color generally shows a value of 50 to 70). L * value was calibrated with a standard white board, and the measurement measured the pigmentation part evenly by repeating a measurement 5 times or more in one site | part. The difference in skin color (ΔL *) at the start and completion of the application was calculated according to Equation 1 shown in Table 4 below. The whitening effect is determined by comparing ΔL * between the sample application site and the control site. When ΔL * value is about 2, the whitening effect of the deposited pigment is clear, and when about 1.5 or more, the whitening effect may be determined. . When the test substance is applied, the L * value is gradually increased, and the comparison between the test substances is calculated according to the following equation (1) by calculating the difference in skin color (ΔL *) at the start and completion of the application. The results are shown in Table 4.

△ L * = L * value 50 days after application-L * value at the start of application

Whitening effect test on human skin Test substance Whitening Effect (△ L *) Example 1 3.65 Comparative Example 1 0.02 Comparative Example 2 2.37 Comparative Example 3 1.38 Comparative Example 4 0.85 Comparative Example 5 2.86 Comparative Example 6 2.69 Comparative Example 7 1.67

As can be seen in Table 4, Comparative Examples 2 to 7 containing one or two selected from the three hundred sec extract, polygamma glutamic acid and Himalayan glacial water of the present invention shows a higher whitening effect than Comparative Example 1 does not contain all Example 1, containing three hundred seconds extract, poly gamma glutamic acid and Himalayan glacial water together showed the best skin whitening effect. Therefore, it was found that the cosmetic composition containing the tritical herb extract, polygamma glutamic acid and Himalayan glacial water according to the present invention as an active ingredient has an excellent skin whitening effect.

Test Example 4 Angular mass reduction effect

In order to determine the angular mass reduction effect of the whitening cosmetic composition according to the present invention, the amount of skin exfoliation was measured using Example 1 and Comparative Examples 1 to 7.

50 adult males and females in their 20s and 30s with no skin disease were asked to apply the compositions of Example 1 and Comparative Examples 1 to 7 to the inside of Habak, and then after 24 hours, using Charm view (Moritex, Japan) The amount of keratin reduction was measured. The initial skin angular mass and angular mass after 24 hours were measured by using the Charm view at constant temperature and humidity conditions (24 ° C., 40% humidity) before the start of application, and the amount of skin dead skin loss was calculated. Indicated.

Skin angular mass change according to whitening cosmetic composition (%) Test substance Angular mass before application Angular mass after 24 hours Skin dead skin cells Example 1 20.2 8.9 11.1 Comparative Example 1 20.4 19.9 0.5 Comparative Example 2 19.7 16.6 3.1 Comparative Example 3 19.9 16.1 3.8 Comparative Example 4 20.2 14.3 5.9 Comparative Example 5 19.9 16.1 3.8 Comparative Example 6 20.1 11.4 8.7 Comparative Example 7 20.2 13.1 7.1

As can be seen in Table 5, Example 1 containing all of the three hundred sec extract, polygamma glutamic acid and Himalayan glacial water of the present invention contains one or two selected from three hundred sec extract, poly gamma glutamic acid and Himalayan glacial water or do not contain all It was confirmed that the skin exfoliation effect is significantly superior to those of Comparative Examples 1 to 7. Therefore, the composition of the present invention was able to improve the rough skin surface by reducing the skin angular mass and make the skin tone look bright overall.

In addition, Comparative Example 4 and Comparative Examples 6 to 7 containing the Himalayan glacier water, which is an active ingredient of the present invention, show a higher amount of skin exfoliation than the composition containing no Himalayan glacier water. It was confirmed that it can be reduced more effectively.

Claims (5)

Cosmetic composition for skin whitening containing three extracts (Saururus chinensis Baill), poly-gamma glutamic acid (Poly-γ-glutamic Acid) and glacial water as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the tritical extract is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The cosmetic composition for skin whitening according to claim 1, wherein the polygamma glutamic acid is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The cosmetic composition for skin whitening according to claim 1, wherein the glacial water is contained in an amount of 0.1 to 80% by weight based on the total weight of the composition. The cosmetic composition for skin whitening according to claim 1, wherein the glacial water is produced in Hunza in Himalayas, Vilcabamba in Ecuador, or Abkhazia in Caucasus.