KR20100040165A - Stock feed composition containing blend of 5-aminolevulinic acid and fermented kitosan as active ingredient - Google Patents

Abstract

PURPOSE: A stock feed composition containing a blend of 5-aminolevulinic acid and fermented chitosan is provided to offer good growth rate and immunity by developing an ALA- fermented chitosan blend as a substitute of antibiotics. CONSTITUTION: A stock feed composition contains 5-aminolevulinic acid and a compounding agent of a fermented chitosan blend as active components. The coupling agent of 0.1~0.2 weight% is added in fodder. The basic feed includes corn 45.23 parts by weight, soybean meal 26.07 parts by weight, whey 16 parts by weight, fish meal 5.00 parts by weight, beef tallow 3.00 parts by weight, calcium phosphate 1.16 parts by weight, molasse 2.5 parts by weight, salt 0.25 parts by weight, vitamin premix 0.12 parts by weight mineral premix 0.10 parts by weight and antioxidant 0.05 parts by weight.

Description

Stock feed composition containing blend of 5-aminolevulinic acid and fermented kitosan as active ingredient}

The present invention relates to an animal feed composition for enhancing immunity and replacing antibiotics, and more particularly, to provide an animal feed containing a combination of 5-aminolevulinic acid (ALA) and a chitosan fermentation as an active ingredient, thereby replacing animal antibiotics. It relates to the contents that can provide a feed having an immune boosting effect and a growth promoting effect.

Along with economic development, the animal life industry has also changed to mass breeding in a narrow space, resulting in various diseases due to stress and immunodeficiency of animals. Antibiotics are abused to prevent or cure them in advance, resulting in a number of side effects such as antibiotic resistance problems and the retention of antibiotics in livestock products.

The EU has already banned the addition of antibiotics to animal feed for the purpose of promoting growth, and the use of antibiotics for the treatment of diseases has also been a strict procedure. The development of drugs that can replace antibiotics for the survival of the animal industry and national health is urgently needed.

To date, a variety of materials have been developed to improve the immunity of animals and to replace antibiotics, but there are many limitations to apply to animals due to uncertainty or low production efficiency.

5-aminolevulinic acid (ALA) is a precursor of heme synthesis in vivo, and has been studied in various fields such as cancer therapy, herbicides, plant growth regulators, and precursors for P450 production. It has been made a lot, and recently has the antibiotic replacement and immune boosting effect of the animal. However, it is not yet clear about the contents which proved a remarkable effect as in the present invention when feeding a feed supplemented with 5-aminolevulinic acid (ALA) and chitosan fermented products to livestock.

The present invention has been made by the above-mentioned demands, and the present invention provides the same growth rate and immunity as when a large amount of antibiotics is formulated by combining ALA-chitosan fermented product mixture with a conventionally prepared feed used in the livestock industry. It is intended to provide a livestock feed composition for the production of safe meat products while producing all livestock, while eliminating all / some of the use of antibiotics that harm the human body ingesting livestock and their meat products.

In order to solve the above problems, the present invention provides a livestock feed composition for immunity enhancement and antibiotic replacement containing a combination of 5-aminolevulinic acid (ALA) and chitosan fermentation as an active ingredient.

The present invention also provides a livestock feed comprising the livestock feed composition.

The present invention can provide a livestock feed composition that enables the production of safe livestock by developing a mixture of ALA-chitosan fermented product which is an antibiotic substitute and a substance for improving animal immunity.

Livestock feed according to the present invention is to mix the ALA-chitosan fermented product mixture in a predetermined proportion to the animal feed according to the growth state of the livestock, it is possible to exclude all of the antibiotics blended into the livestock feed.

The ALA-chitosan fermentation mixture applied in the present invention is a combination of 5-aminolevulinic acid (ALA) and chitosan fermentation. 5-aminolevulinic acid (ALA) is a precursor of heme synthesis in vivo, and has been used as a cancer treatment agent, herbicide, plant growth regulator, precursor for P450 production, and chitosan fermentation products are chitosan and chitosan. Oligosaccharides are fermented with Bacillus MJ-1 strain (KCTC 18116P, Korean Patent Application No. 2006-0076392) to enhance the immunity of the livestock and improve the livestock productivity.

In addition, domesticated cattle are at risk of many diseases, and vaccines are often administered to prevent them, which can cause economic losses for livestock farmers because of the slow growth of these vaccines. On the other hand, since the addition of the ALA-chitosan fermented product mixture as in the present invention has an effect of increasing the immunity can reduce the frequency of disease can improve the productivity of the livestock. In other words, the ALA-chitosan fermentation mixture itself can increase the immunity of livestock and prevent the disease of livestock even if it restricts the use of artificial antibiotics and anti-inflammatory agents in small amounts. This will not contribute to the production of high quality livestock and thus will contribute greatly to the production of superior livestock and high quality pork.

Hereinafter, the content of the present invention will be described in more detail.

The present invention provides a feed composition for improving immunity and antibiotic replacement containing a combination of 5-aminolevulinic acid (ALA) and chitosan fermentation as an active ingredient.

The mixture according to one embodiment of the present invention is to be added to the basic feed in 0.1 to 0.2%, preferably to 0.2%.

The basic feed according to an embodiment of the present invention is characterized in that the main ingredient consists of corn, soybean meal, whey, fish meal, molasses, salt, vitamin premix and mineral premix. Vitamin premixes may consist of vitamin A, vitamin D, vitamin E, riboprabin and niacin, and mineral premixes may consist of manganese, iron zinc, calcium, copper, cobalt and selenium.

The basic feed according to an embodiment of the present invention is 45.23 parts by weight of corn, 26.07 parts by weight of soybean meal, 16 parts by weight of whey, 5.00 parts by weight of fishmeal, 3.00 parts by weight, 0.52 parts by weight of limestone, phosphoric acid based on 100 parts by weight of basic feed It is characterized in that the weaning pig feed consisting of the composition of 1.16 parts by weight of calcium, 2.5 parts by weight of molasses, 0.25 parts by weight of salt, 0.12 parts by weight of vitamin premix, 0.10 parts by weight of mineral premix and 0.05 parts by weight of antioxidant. Ethoxyquin may be used as the antioxidant, but is not limited thereto.

The present invention also provides a livestock feed comprising the livestock feed composition.

Hereinafter, the contents of the present invention will be described in more detail with reference to Examples. However, these examples are only presented to understand the content of the present invention, but the scope of the present invention is not limited to these embodiments.

<Example 1. ALA added to feed alone to feed livestock>

In the case of ALA, it has a positive effect on digestibility as shown in Table 1 below, and the possibility of anti-inflammatory action in inducing an inflammatory response has been published in a foreign paper (Livestock science, 114 (2008) 108-116).

The addition of ALA in weaned pigs was significantly increased in DM (building) and N (nitrogen, protein) digestibility at 35 days after the test (p <0.01).

Induction of LPS (Acute Inflammatory Response) with or without ALA in weaned pigs was shown to reduce the blood Cortisol (stress hormone) concentration at 2 hours of inoculation (p <0.01), and at 12 hours Tendency was shown (p = 0.07). The IGF-I concentration was higher in the ALA-added group at 2 hours (p <0.05). In addition, TNF-α (tumor necrosis factor) was significantly lower at 2 hours after addition of ALA (p <0.05) and at 4 hours (p = 0.09) (Table 2). This makes it possible to see that ALA raises the immune function of weaning piglets.

<Example 2. Chitosan fermented product added to feed alone when fed to livestock>

In addition, chitosan fermented products have been reported to have a positive effect on growth and digestion rate as described below (Jin et al., 2008;

As shown in Table 3, the effect of the addition of chitosan fermentation in the weaning pig feed on growth was increased by 15-28 days after the test and as the level of chitosan fermentation increased before the test (0-28 days). ) And ADFI (daily feed intake) tended to increase (p <0.10).

As shown in Table 4, the effect of the addition of chitosan fermented product to weaning pig feed on nutrient digestibility was not added when 5 g / kg of chitosan fermented product was added at the end of the test (28 days) in DM (building) and N (nitrogen, protein) digestibility. The digestibility was significantly higher (p <0.05). In addition, the digestibility increased with increasing chitosan fermentation level in dry matter digestibility (p <0.05), and the digestibility increased with increasing level of chitosan fermentation in nitrogen digestion rate (p <0.10).

<Example 3. Efficacy measuring method of ALA-chitosan fermented product mixture>

120 heads of three-way hybrids (Duroc × Yorkshire × Landrace) were disclosed. The weight of the test was 7.10 kg. The specification test was conducted in the experimental research building of Dankook University. As shown in Table 5, 0.1% of ALA-chitosan fermented product mixture was added to corn and soybean meal-based basic feeds (antibiotic spheres), antibiotics containing 40 ppm of avilamycin and 100 ppm of oxytetracycline, and non-antibiotic feed. The treatments were treated with four mouths containing 0.2% ALA-chitosan fermented product mixture in the mouth and the antibiotic-free diets, and the cells were randomly placed in six repeats and five heads per treatment.

Corn and wheat are common sources of energy, and soybean meal without soybeans, whey and by-products left after cheese processing are dried and ground fish meal is a protein source. Uji is a source of energy supplemented with oil extracted from livestock fats. Calcium phosphate and limestone are the sources of calcium and phosphorus that make up bone. Vitamin premixes may consist of vitamin A, vitamin D, vitamin E, riboprabin and niacin, and mineral premixes may consist of manganese, iron zinc, calcium, copper, cobalt and selenium.

In addition, the basic feed contains 3,500 kV metabolic energy / kg, 21.52% crude protein, 1.33% lysine, 0.75% calcium (Ca), and 0.64% phosphorus (P) based on the NRC (1998) specification. It was. The test feed was made free of vegetarian food in the form of powder feed, and the water was freely eaten by using an automatic water dispenser.

Table 5. Feed Mixing Ratio

After 42 days of feeding the test feed, the daily weight gain (ADG), daily feed intake (ADFI), feed efficiency (G / F), general ingredient digestibility, and blood immunoassay were measured. It was carried out by conventional methods in the art.

The body weight and feed intake were measured at the beginning of the test, week 1, 3, and 6 (end) to calculate the daily gain, feed intake, and feed efficiency based on the difference in body weight and feed intake. Dry matter and nitrogen digestibility were measured over three and six weeks (end). Digestibility was measured 7 days before digestion was measured by adding 0.2% of Cr ₂ O ₃ feed fluorinated substances in the feed and dry matter and nitrogen digestibility was measured. Serum and plasma were collected from the jugular vein at the beginning and end of the test, and red blood cells (RBC), white blood cells (WBC), lymphocytes (Lymphocyte), hemoglobin (Hb), iron (Iron), iron binding capacity (TIBC) Was analyzed.

Example 4 Influence of ALA-chitosan Fermentation Mixture on Growth Capacity

The effect of the addition of ALA-chitosan fermented product mixture on weaning pig feed is shown in Table 6. The daily gain for 0-7 days was significantly increased in the ANT treatment compared to the control (p <0.05). For 8-21 days, the daily gain increased significantly in the ANT and AF2 treatments compared to the control (p <0.05), and the control in the daily intake was significantly lower than the other treatments (p <0.05). The daily gain was 22-42 days in ANT and AF2 treatments (p <0.05), and AF1 treatments were significantly higher than control (p <0.05). In addition, ANT treatment was significantly higher in the feed efficiency than the control (p <0.05). The ANT and AF2 treatments were significantly higher than the control group (p <0.05) and the ANT treatments were higher than the control group (p <0.05).

In the treatment of existing chitosan fermented products, the addition of 2.5g / kg (0.25%) increased the daily gain by 3% and the daily feed intake by 4.2%, and the addition of 5g / kg increased 6.6% and 7.2%, respectively. On the other hand, the ALA-chitosan fermented product mixture showed a 6.7% and 6.8% improvement in daily gain and feed efficiency with 0.1% addition and 11.3% and 13.1% improvement with 0.2% addition.

Table 6. Impact on growth capacity

<Example 5. General ingredient digestibility of ALA-chitosan fermented product mixture>

The effect of the addition of ALA-chitosan fermented product mixture on weaning piglet diets is shown in Table 7. At week 1, the ANT and AF2 treatments showed the highest digestibility (P <0.05), and the AF1 treatments were significantly higher than the control (P <0.05). In the nitrogen digestibility (N), the control showed the lowest results (P <0.05), and the AF1 treatment was significantly lower than the ANT treatment (P <0.05). At 3 weeks of building digestibility, the control was significantly lower than the other treatments (P <0.05), and the ANT and AF2 treatments were significantly higher than the control (P <0.05). At the end of the treatment, ANT and AF2 treatments were higher than control (P <0.05), and nitrogen control rate was the lowest (P <0.05).

In addition, 5 mg / kg of total ALA treatment resulted in 1.3% and 0.6% improvement in dry matter and nitrogen digestibility, 2.3% and 1.4% in 10mg / kg, and 6.5% and 5.6% in 15mg / kg. . In addition, the addition of 2.5g / kg (0.25%) improved 3.5% and 3.7% for chitosan fermentation, and 6.6% and 8.6% for 5g / kg (0.5%).

In the case of ALA-chitosan fermentation mixture, 0.1% addition of 1% at the beginning of the test showed 14.5% and 23.3% increase in dry matter and nitrogen digestibility, and 0.2% addition showed 18.0% and 28.2% improvement in digestibility.

Table 7. Effect on building and nitrogen digestibility

Example 4 Effect of ALA-chitosan Fermentation Combination on Blood Immune Indicators

The effect of the addition of ALA-chitosan fermented product mixture in weaned pig diet on the immunomarker in blood is shown in Table 8. RBC, WBC, Hb, Iron, and TIBC showed no difference among treatments (P> 0.05). However, the control group showed significantly higher results in lymphocyte content than the AF1 and AF2 treatments with ALA-chitosan fermentation mixture (P <0.05).

In the case of ALA-chitosan fermentation mixture, lymphocytes showed 72.3% level when 0.1% was added at the end of the test, and 76.6% level when 0.2% was added, and the effect of boosting immunity could be confirmed by adding ALA chitosan fermentation mixture. there was.

Table 8. Effect on Blood Immune Indicators

Claims (6)

Immunity enhancement and antibiotic replacement animal feed composition comprising a combination of 5-aminolevulinic acid (ALA) and chitosan fermentation as an active ingredient. The livestock feed composition according to claim 1, wherein the mixture is added in an amount of 0.1 to 0.2% by weight to the basic feed. The livestock feed composition according to claim 2, wherein the mixture is added in an amount of 0.2% by weight to the basic feed. The livestock feed composition of claim 2 or 3, wherein the basic feed comprises corn, soybean meal, whey, fish meal, molasses, salt, vitamin premix and mineral premix. According to claim 4, wherein the basic feed is 45.23 parts by weight of corn, 26.07 parts by weight of soybean meal, 16 parts by weight of whey, 5.00 parts by weight of fish meal, 3.00 parts by weight of whey, limestone 0.52 parts by weight, calcium phosphate 1.16 Livestock feed composition, characterized in that the feed composition consisting of parts by weight, 2.5 parts by weight molasses, 0.25 parts by weight of salt, 0.12 parts by weight of vitamin premix, 0.10 parts by weight of mineral premix and 0.05 parts by weight of antioxidant. Livestock feed comprising the livestock feed composition of any one of claims 1 to 5.