KR20200102648A - 5-Adding livestock feed containing the efficacy of a mixture of aminorevulcanic acid (ALA) and kittoic acid ferments - Google Patents

Abstract

The present invention relates to a livestock feed composition containing a mixture of 5-aminolevulinic acid (ALA) and chitosan fermentation product as an active component. More specifically, the present invention relates to: a livestock feed composition for improving immunity and replacing antibiotics, which contains a mixture of ALA and chitosan fermentation product as an active component; and livestock feed containing the livestock feed composition.

Description

5-Adding livestock feed containing the efficacy of a mixture of aminorevulcanic acid (ALA) and kittoic acid ferments}

The present invention relates to a livestock feed composition for enhancing immunity and replacing antibiotics, and more particularly, by providing livestock feed containing a mixture of 5-aminolevulinic acid (ALA) and fermented chitosan as an active ingredient, replacing antibiotics in animals, It relates to the content that can provide a feed that has the effect of increasing immunity and promoting growth.

With the economic development, the animal production industry has also changed to a form of mass rearing in a narrow space, and various diseases have occurred due to the occurrence of stress and immune deficiency in animals. Antibiotics are abused in order to prevent or treat them in advance, resulting in various side effects such as antibiotic resistance problems and residual antibiotics in livestock products.

It has reached a level that threatens health.

The European Union (EU) has already banned the addition of antibiotics to animal feed for the purpose of promoting growth, and the use of antibiotics for disease treatment is also subject to strict procedures, and the Korean government is also pursuing policies in the same direction. For the survival of the animal industry and the health of the public, there is an urgent need to develop a substitute for antibiotics.

Until now, various materials having the effect of improving the immunity of animals and replacing antibiotics have been developed, but there are many limitations to be applied to animals because the efficacy is not certain or the production efficiency is low.

5-aminolevulinic acid (ALA) is a precursor of heme synthesis in vivo, and has been studied in various fields such as cancer treatment, herbicide, plant growth regulator, and precursor for P450 production. It has been made a lot, and recently, has the effect of replacing antibiotics and enhancing immunity in animals. However, when the feed to which the 5-aminolevulinic acid (ALA) and chitosan fermented product are added together is fed to livestock, the contents that proved the remarkable effect as in the present invention have not been revealed yet.

The present invention was conceived by the above demands, and the present invention provides an excellent growth rate and immunity as when a large amount of antibiotics are blended by blending an ALA-chitosan fermented product mixture into a conventional feed used in the livestock industry. While producing livestock, it is intended to provide a livestock feed composition for the production of safe meat products by excluding all/part of the use of antibiotics that harm livestock and the human body ingesting the meat products.

In order to solve the above problem, the present invention contains a mixture of 5-aminolevulinic acid (ALA) and chitosan fermented product as an active ingredient.

Provides a livestock feed composition for improving immunity and replacing antibiotics.

The present invention also provides a livestock feed comprising the livestock feed composition.

The present invention can provide a livestock feed composition that enables safe livestock production by developing an ALA-chitosan fermented product mixture, which is a substance for improving livestock immunity and an antibiotic substitute substance.

No drawing. Replaced by table.

The livestock feed according to the present invention is to be able to exclude all of the antibiotics blended in livestock feed by blending the ALA-chitosan fermented product mixture into the livestock feed at a certain ratio according to the growth state of the livestock.

The ALA-chitosan fermented product mixture applied in the present invention is a mixture of 5-aminolevulinic acid (ALA) and chitosan fermented product. 5-Aminolevulinic acid (ALA) is a precursor of heme synthesis in vivo, and has functions as cancer treatment agents, herbicides, plant growth regulators, and precursors for P450 production. Chitosan fermented products are chitosan and chitosan. Oligosaccharides Bacillus MJ-1 strain

(KCTC 18116P, Korean Patent Application No. 2006-0076392) fermented to improve livestock productivity by enhancing the immunity of livestock.

In addition, livestock reared are at risk of many diseases, and vaccines are often administered to prevent this, which causes economic loss for livestock farmers because such vaccines cause a decrease in growth. As in the invention, the addition of the fermented ALA-chitosan mixture has the effect of enhancing immunity.

It is possible to improve the productivity of livestock by reducing the frequency of disease. In other words, the ALA-chitosan fermented product mixture itself increases the immunity of livestock and prevents livestock diseases even if the use of artificial antibiotics and anti-inflammatory drugs is limited to a small amount, as well as the concentration of harmful substances in the human body that consumes the livestock. It does not cause the production of high-quality livestock and, accordingly, can greatly contribute to the production of high-quality pork.

Hereinafter, the content of the present invention will be described in more detail.

The present invention provides a livestock feed composition for enhancing immunity and replacing antibiotics, comprising a mixture of 5-aminolevulinic acid (ALA) and chitosan fermented product as an active ingredient.

The mixture according to an embodiment of the present invention is added to the basic feed in an amount of 01 to 02%, but is preferably added in an amount of 02%.

The basic feed according to an embodiment of the present invention is characterized in that the main ingredients are corn, soybean meal, whey, fish meal, molasses, salt, vitamin premix and mineral premix. The vitamin premix may be composed of vitamin A, vitamin D, vitamin E, riboflavin, and niacin, and the mineral premix may be composed of manganese, iron zinc, calcium, copper, cobalt and selenium.

The basic feed according to an embodiment of the present invention is 4523 parts by weight of corn, 2607 parts by weight of soybean meal, 16 parts by weight of whey, 500 parts by weight of fish meal, 300 parts by weight of tallow, 052 parts by weight of limestone, phosphoric acid based on 100 parts by weight of the basic feed. Calcium 116 parts by weight, molasses 25 parts by weight, salt 025 parts by weight, vitamin premix 012 parts by weight, mineral premix 010 parts by weight, and antioxidant 005 parts by weight

It characterized in that it is a weaning pig feed consisting of the composition of. Ethoxyquin may be used as the antioxidant, but is not limited thereto.

The present invention also provides a livestock feed comprising the livestock feed composition.

Hereinafter, the content of the present invention will be described in more detail through examples. However, these examples are only presented to understand the content of the present invention, and the scope of the present invention is not limited to these examples.

<Example 1 When ALA alone was added to feed and fed to livestock>

In the case of ALA, as shown in Table 1 below, it has a positive effect on digestibility, and the possibility of anti-inflammatory effect when inducing an inflammatory response has been announced through foreign papers (Livestock science, 114(2008) 108-11).

When ALA was added to the feed for weaning piglets, as the level of ALA increased on the 35th day after the test, the digestibility of DM (dry) and N (nitrogen, protein) significantly increased (p<001).

The effect of reducing the concentration of Cortisol (stress hormone) in the blood at 2 hours of inoculation was shown when inoculating LPS (a substance expressing acute inflammatory reaction) according to the presence or absence of ALA in the feed of weaning piglets (p<001). Showed a tendency

(p=007) The concentration of IGF-I (growth-promoting hormone) was also high in the case of ALA-added at 2 hours (p<005) In addition, in TNF-α (tumor necrosis factor), the ALA-added group was significantly higher at 2 hours Was low (p<005), and showed a low trend at 4 hours.

(p = 009) (Table 2) This makes it possible to know that ALA increases the immune function of weaning piglets.

<Example 2 When chitosan fermented product alone was added to feed and fed to livestock>

In addition, in the case of chitosan fermented products, it is reported that it acts positively on growth and digestibility as follows.

Looking at the effect of the addition of chitosan fermented product in the feed for weaning piglets on growth, as shown in 3, until 15-28 days after the test and the period before the test (0-28 days), ADG (per day Weight gain) and ADFI (daily feed intake) tended to increase (p<010).

As shown in Table 4, the effect of the addition of chitosan fermented product in the feed for weaning piglets on nutrient digestibility was observed at the end of the test (28 days) when 5g/kg of chitosan fermented product was added at the digestibility of DM (building) and N (nitrogen, protein). In addition, the digestibility increased as the addition level of chitosan fermented product increased in dry matter digestibility (p<005), and the digestion rate increased as the addition level of chitosan fermented product increased in nitrogen digestibility. Tended to increase

<Example 3 Method for measuring the efficacy of ALA-chitosan fermented product mixture>

120 piglets of three-way hybrid (Duroc×Yorkshire×Landrace) weaning were disclosed, and the weight at the start of the test was 710kg.

The specification test was conducted in the experimental research building of Dankook University. As shown in Table 5, the test design was based on corn and soybean meal-based feed (antibiotic-free), antibiotics with 40 ppm avilamycin and 100 ppm of oxytetracycline, and 01% of ALA-chitosan ferment mixture added to the antibiotic-free feed. The bulbs were treated with 4 bulbs containing 02% of the fermented ALA-chitosan mixture added to the diet without antibiotics and were completely randomly arranged for 6 repetitions per treatment and 5 heads per repetition.

Corn and wheat in general feed are energy sources, and soybean meal minus oil from soybeans, fish meal crushed after drying the fish meal, which is a by-product remaining after processing cheese, is a protein source. Tallow is a raw material that supplements energy with oil extracted from livestock fat, and calcium phosphate and limestone are raw materials that supply calcium and phosphorus that make up bones. The vitamin premix may be composed of vitamin A, vitamin D, vitamin E, riboflavin, and niacin, and the mineral premix may be composed of manganese, iron zinc, calcium, copper, cobalt and selenium.

In addition, the basic feed, based on the specification standard of NRC (1998) 3,500 ㎉ metabolic energy / kg, 2152% crude protein, 133% lysine (lysine), 075% calcium (Ca), 064% phosphorus (P) To contain. Test feed is free vegetarian in the form of powdered feed

The water was freely eaten using an automatic water supply.

Table 5 Feed Mixing Ratio

After feeding the test feed for 42 days, the daily gain (ADG), daily feed intake (ADFI), feed efficiency (G/F), general ingredient digestibility, and blood immunity indicators were measured for each treatment group.The measurement method is as follows. It was carried out in a conventional manner in the art.

Weight and feed intake were measured at the start of the test, week 1, week 3, and week 6 (at the end), and the daily weight gain, daily feed intake, and feed efficiency were calculated based on the difference in weight and feed intake. Dry matter and nitrogen digestibility were measured over three and six weeks (end). From 7 days before the digestibility measurement, after feeding 02% of Cr2O3, a fluorinated substance in feed, food was collected and the digestibility of dry matter and nitrogen was measured. At the start and end of the test, serum and plasma are collected from the jugular vein to determine red blood cells (RBC), white blood cells (WBC), lymphocytes (Lymphocytes), hemoglobin (Hb), iron (Iron), and iron binding capacity (TIBC). analysis

I did.

<Example 4 Effect of ALA-chitosan fermented product mixture on growth capacity>

The effect of the addition of ALA-chitosan fermented product mixture in the weaning pig feed on the growth capacity is shown in Table 6. For 0-7 days, the daily gain was significantly increased in ANT treatment compared to the control (p<005) During 8-21 days, the daily gain was significantly increased in ANT and AF2 treatment compared to the control (p<005) 005), in terms of daily feed intake, the control group was significantly lower than that of the other treatments (p<005). The daily gains for 22-42 days were the highest in the ANT and AF2 treatments (p<005), and the AF1 treatment was more significant than the control. In addition, in terms of feed efficiency, ANT treatment was significantly higher than that of control (p<005) In terms of daily weight gain during the total feeding period (0-42 days), ANT and AF2 treatments were more significant than control. Was high (p<005), and in feed efficiency, ANT treatment was higher than that of control (p<005).

[0042] When treating the existing chitosan fermented product, when 25g/kg (025%) was added during the entire test period, it showed an increase of 3% in daily weight gain, and an increase in published patent 10-2010-004016542% in daily feed intake, and 5g/kg added In the case of ALA-chitosan fermentation mixture, 67% and 68% increase in daily weight gain and feed efficiency when 01% is added, and 113% and 131% are improved when 02% is added. Showed effect.

Table 6 Effect on growth ability

[0044]

<Example 5 Digestibility of general ingredients of ALA-chitosan fermented product mixture>

The effect of the addition of ALA-chitosan fermented product mixture in the weaning pig feed on the digestibility of general ingredients is shown in Table 7. At week 1 dry matter digestibility (DM), the ANT and AF2 treatments showed the highest digestibility (P<005), and the AF1 treatment was significantly higher than the control (P<005). In the nitrogen digestibility (N), the control was the lowest. (P<005), and AF1 treatment was significantly lower than ANT treatment (P<005). In the dry matter digestibility at week 3, the control was significantly lower than other treatments (P<005), and ANT in nitrogen digestibility. And AF2 treatments were significantly higher than the control (P<005). At the end, the dry matter digestibility of ANT and AF2 treatments were higher than that of the control (P<005), and the nitrogen digestibility of the control was the lowest (P<005).

[0047] Existing ALA treatment, 13%, 06% improvement in dry matter and nitrogen digestibility when 5mg/kg is added during the entire test period, 23% when 10mg/kg is added, 14% is improved, 65%, 56% when 15mg/kg is added Showed improvement. In addition, when chitosan fermentation was treated, when 25g/kg (025%) was added, it improved 35% and 37%, and when 5g/kg (05%) was added, it improved 66% and 86%.

[0048] In the case of the ALA-chitosan fermented product mixture, when 01% was added to the first week of the test, it showed an increase in dry matter and nitrogen digestibility of 145% and 233%, and when 02% was added, the digestibility was improved by 180% and 282%. Showed.

<Example 5 Digestibility of general ingredients of ALA-chitosan fermented product mixture>

The effect of the addition of ALA-chitosan fermented product mixture in the weaning pig feed on the digestibility of general ingredients is shown in Table 7. At week 1 dry matter digestibility (DM), the ANT and AF2 treatments showed the highest digestibility (P<005), and the AF1 treatment was significantly higher than the control (P<005). In the nitrogen digestibility (N), the control was the lowest. (P<005), and AF1 treatment was significantly lower than ANT treatment (P<005). In the dry matter digestibility at week 3, the control was significantly lower than other treatments (P<005), and ANT in nitrogen digestibility. And AF2 treatments were significantly higher than the control (P<005). At the end, the dry matter digestibility of ANT and AF2 treatments were higher than that of the control (P<005), and the nitrogen digestibility of the control was the lowest (P<005).

[0047] Existing ALA treatment, 13%, 06% improvement in dry matter and nitrogen digestibility when 5mg/kg is added during the entire test period, 23% when 10mg/kg is added, 14% is improved, 65%, 56% when 15mg/kg is added Showed improvement. In addition, when chitosan fermentation was treated, when 25g/kg (025%) was added, it improved 35% and 37%, and when 5g/kg (05%) was added, it improved 66% and 86%.

[0048] In the case of the ALA-chitosan fermented product mixture, when 01% was added to the first week of the test, it showed an increase in dry matter and nitrogen digestibility of 145% and 233%, and when 02% was added, the digestibility was improved by 180% and 282%. Showed.

Replaced by table.

Claims (1)

5-Aminolevulinic acid (ALA) and chitosan fermented animal feed composition for improving immunity and replacing antibiotics, characterized in that it contains a mixture as an active ingredient.

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