KR20100000025A - Composition for skin whitening - Google Patents

Abstract

PURPOSE: A composition for skin whitening, which contains pig placental extract is provided to suppress melanogenesis and ensure high skin whitening effect with high safety. CONSTITUTION: A composition for skin whitening contains pig placental extract isolated by ultrasonic wave. The placental extract is: an extract obtained by mixing water, ethanol, or their mixture solvent to pig placental powder and irradiating ultrasonic wave; a powder extract which is removed from placental ground material; an extract of supernatant after centrifuging placental ground material; a powder extract obtained by centrifuging placental ground material and removing solvent from the supernatant; an extract of supernatant obtained by filtering placental ground material and centrifuging; and a powder resultant obtained by filtering placental ground material, removing extract, centrifuging, and removing solvent from supernatant. The composition is a cosmetic composition or pharmaceutical composition.

Description

Composition for Skin Whitening

The present invention relates to a skin lightening composition. Specifically, the present invention relates to a whitening composition using the pig placenta extract.

Many people want to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also affected by environmental or physiological conditions such as sun ultraviolet rays, skin and stress.

Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction.

Until now, the development of the whitening agent has been mainly to find a substance that reduces the amount of melanin by inhibiting the activity of tyrosinase, the basic and most important enzyme in melanin biosynthesis. Whitening agents developed in this way include Kojisan (Korean Patent 147412, 309398), Sichuan Mushroom Extract (Korean Patent 206538), Mulberry Eggplant Extract (Korean Patent 253842), Black Bean Extract (Korean Patent 281011), Yulpi Extract (Korean Patent 253843) Although, white bran extract (466379), glutathione, vitamin A, vitamin C, etc., but does not obtain a whitening effect satisfactory to the consumer, there is a limit to the amount of use due to the occurrence of side effects to increase the effect. In addition, in the case of plant extracts have a disadvantage that shows a relatively high effect only at high concentrations, the scope of application is very limited. Therefore, it is still required from the user's point of view to develop a whitening agent that is excellent in whitening efficacy and is safe for use.

An object of the present invention to provide a composition for skin whitening using pig placenta extract.

Specific aspects or other objects of the present invention will be presented below.

The inventors of the present invention, as confirmed in the following examples and experimental examples, prepared a placenta extract by ultrasound, and through clinical trials confirmed that the extract has a whitening effect and wrinkle improvement effect.

The present invention is provided based on these experimental results.

Therefore, in one aspect, the present invention can be understood as a skin whitening composition comprising the pig placenta extract by ultrasound as an active ingredient, and in another aspect, a composition for improving wrinkles comprising the pig placenta extract by ultrasound as an active ingredient You can figure it out.

The following terms have the meanings defined below.

<1> skin whitening

As used herein, "skin whitening" can be understood as the resulting phenomenon as the synthesis of melanin is inhibited. As disclosed in the following Examples and Experimental Examples, the present inventors have found that the pig placenta extract, an active ingredient of the composition of the present invention, has melanin synthesis inhibitory activity in an in vitro experiment (experiment using B16 melanoma cell line). I confirmed it.

As a result of the inhibition of the synthesis of melanin, specifically, the synthesis of the melanin means that the symptoms resulting from the increase of melanin are alleviated or improved.

It is known that melanin causes skin aging when it is generated more than necessary, and in particular, hyperpigmentation such as blemishes and freckles. Therefore, the symptoms resulting from the increase of melanin include blemishes, freckles, skin aging and the like.

<2> skin wrinkles

Skin wrinkles are generally caused by a decrease in collagen or elastin, which maintains skin elasticity by UV rays, various harmful substances, and active oxygen (Ingrid Emerit et al. Free radical). and aging, 1992; Arther K. Balin et al. Aging and the skin, 1998).

As used herein, the meaning of skin wrinkles refers to all types of skin wrinkles regardless of what causes (eg, ultraviolet rays, natural aging, free radicals, various harmful substances that cause free radicals in the body, etc.).

<3> placenta

The placenta is generally formed from tissues derived from the fetus and the mother. The placenta is composed of the decidual membrane derived from the uterine mucosa of the mother and the chorion and amniotic membrane derived from the fertilized egg, and refers to the organ where the material exchange between the fetus and the mother occurs.

Porcine placenta as an extraction subject in the present invention will usually be washed and then lyophilized.

<3> Ultrasonic Pig Placenta Extract

It means that the placenta is crushed by adding water, ethanol or a mixed solvent thereof to the freeze-dried and powdered pork placenta powder, and radiating ultrasonic waves. Preferably, the placenta is crushed and then lyophilized in the crushed product. Refers to an extract in powder form from which the solvent is removed. More preferably, the placenta is crushed, and the crushed material is centrifuged to take a supernatant, and the powdered extract from which the solvent is removed from the supernatant by lyophilization or the like, or the pulverized placenta and filtered to remove the extraction residue. After that, the filtrate is centrifuged and refers to an extract in the form of a powder in which the solvent is removed by a method such as lyophilization. Preferably what is obtained by the process of the following Example and its process conditions.

<4> improvement

Improvement herein is meant to include alleviation of symptoms, prevention and / or treatment.

<5> active ingredients

An active ingredient refers to an ingredient which shows activity alone or in itself, together with an inactive adjuvant (carrier).

Meanwhile, the composition of the present invention (skin whitening composition or composition for improving skin wrinkles; the same as below) may be any amount as long as the placenta extract, which is an active ingredient thereof, can exhibit skin whitening or wrinkle improvement effect depending on the use, formulation, and compounding purpose. (Effective amount), a conventional effective amount will be determined within the range of 0.001% to 15% by weight based on the total weight of the composition. The term "effective amount" refers to the amount of the active ingredient that can induce skin whitening, skin wrinkle improvement (prevention of skin wrinkles, treatment or alleviation of such symptoms) in a person who is subject to the application (prescription). Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The composition of the present invention may be regarded as a pharmaceutical composition in specific embodiments.

The pharmaceutical composition of the present invention includes oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (sterile), in addition to pharmaceutically acceptable carrier placental extracts, as an active ingredient. Aqueous or oily suspensions for injection), topical formulations (solutions, creams, ointments, gels, lotions, patches) and the like. Preferably when prepared in a topical formulation.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) does not have more toxicity (sufficiently low toxicity) to which the subject of application (prescription) is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.) malt, gelatin, talc, solids Lubricants (e.g. stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (e.g. peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g. propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g. TWEENS), wetting agents (e.g. Sodium lauryl sulfate), colorants, flavoring agents, tableting agents, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions and the like. Such a carrier may be used by selecting one or more appropriate ones according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethylcellulose And suspending agents and dispersing agents such as sodium alginate and polyvinylpyrrolidone. Suitable excipients when prepared as injections include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, preferably when administered topically.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

The composition of this invention can be grasped | ascertained as a cosmetic composition in a specific aspect.

Cosmetic compositions of the present invention can be prepared in a variety of forms, including emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (injury and water-based), anhydrous products (oils and glycols) , Gels, masks, packs, powders and the like formulation.

The cosmetic composition of the present invention may include an acceptable carrier in the cosmetic preparation in addition to the pig placenta extract.

As used herein, "acceptable carrier in cosmetic formulation" means a known or used cosmetic or composition that can be included in cosmetic formulations or a compound or composition that will be developed in the future, such that toxicity, instability or irritation is greater than the body can adapt to in contact with skin. Say that there is no.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, preservatives, stabilizers, sunscreens, colorants, fragrances and the like. Such alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, preservatives, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances and the like are already known in the art. Those skilled in the art can select and use appropriate materials / compositions as appropriate.

The carrier may comprise from about 1% to about 99.99% by weight, preferably from about 40% to about 99.99% by weight of the composition based on the total weight of the cosmetic composition of the present invention.

However, since the ratio will vary depending on the formulation as described above in which the cosmetic composition of the present invention is prepared and its specific application site (face, neck, etc.) or its preferred application amount, the ratio is in any aspect to the present invention. It should not be understood as limiting the scope of.

In addition, the composition of this invention can be grasped | ascertained as a soap composition in a specific aspect.

When the composition of the present invention is identified as a soap composition, the soap composition of the present invention may be prepared by including a pig placenta extract which is an active ingredient on a soap base, and prepared as an additive including a skin moisturizer, an emulsifier, a hard water softener, and the like. Can be.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, waxes, fatty alcohols, hydrocarbons, natural Plant extracts and the like can be used, and tetrasodium idieti and the like can be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally added in an amount of 99.999 wt% to 50 wt% based on the total weight of the soap composition. The additive may be added in an amount of 1% to 20% by weight.

As described above, according to the present invention, a skin whitening composition using a pig placenta and a composition for improving skin wrinkles are provided.

Hereinafter will be described with reference to Examples and Experimental Examples of the present invention. However, the scope of the present invention is not limited by these examples and experimental examples.

EXAMPLES Preparation of Porcine Placenta Extract

Washing solution was added to 100 g of lyophilized pig placenta and washed 4 to 5 times. After washing, 400 ml of a hydration solution (50 mM Tris-Cl (pH7.5) containing 1 mM EDTA, 1 mM 2-mercaptoethanol) was added, and the placenta was crushed using an ultrasonic cell grinder in an ice bath for 5 minutes (10 seconds on). , Repeat 10 seconds off). The filtrate was collected by filtration with four layers of guaze, and the filtrate was centrifuged at 700 rpm for 5 minutes to recover the supernatant first. Then, the recovered supernatant was added to Cetriprep YM-50 (50,000 NMWL) and 3,000 rpm, 90 Centrifugation in minutes gave 130 ml.

Preparation Example Preparation of Cream Using Porcine Placenta Extract

Using the extract of the above Example to prepare a cosmetic composition with the ingredients and contents of Table 1 below.

TABLE 1

Ingredients and Contents of Cosmetic Compositions

ingredient Content (% by weight) glycerin 3.00 1,3-butylene glycol 4.00 Sepiplus 400 ((polyacrylate-13 / polyisobutene / polysorbate) 0.40 EDTA-2Na (disodium EDTA) 0.01 Simulsol 165 (PEG-100 Stearate / glyceryl stearate) 1.50 Montanov 202 (arachidyl alcohol / beheny alcohol / arachidyl glucoside) 2.50 Montanox 60 (polysorbate 60) 0.30 Stearic acid 0.50 Nafol 1822B; behenyl alcohol 2.00 Nexbase 2004FG (hydrogenated polydecene) 3.00 Migliol (Miglyol 8810; butylene glycol dicaprylate / dicaprate) 6.00 DC200 (100cs) (dimethicone) 0.50 Example extract 2.00 Symdiol 68T; 1,2-hexanediol / caprylyl glycol / tropolone 0.50 Medicine 0.20 Dipotassium glycyrrhizate (DPG) 0.10 Purified water Remaining amount

Experimental Example Whitening Effect and Wrinkle Removal Effect

Experimental Example 1 Whitening Effect Experiment

<Experimental Example 1-1> In vitro effect of inhibiting melanin production in vitr o

The inhibitory effect of melanin production was tested using B16 melanoma cell line. B16 melanoma cell lines were cultured at 37 ° C. and 5% CO ₂ with DMEM (Dulbecco's modified Eagle's media) in a medium containing 10% fetal bovine serum. After incubating the cells at a concentration of 10 ⁵ cells / well in a 24-well plate and confirming the cell attachment, the extracts of the above examples were added to the cell culture solution at 0.1% and incubated for 3 days.

After removing the culture solution, washed with PBS and dissolved the cells with 1N sodium hydroxide to measure the absorbance at 400nm, the melanin production inhibition rate was calculated according to the following formula and the results are shown in <Table 1>.

Melanin production inhibition rate (%) = 100- (absorbance of test substance / absorbance of control X 100)

TABLE 2

Substance (treatment concentration) Melanin production inhibition rate (%) Extract 0.1% 18.2 Control 0

Table 2 shows that the extracts of the examples show the effect of inhibiting melanin production of pigment cells.

Experimental Example 1-2 Clinical Study on the Whitening Effect

Clinical evaluation was performed by Ellid Co., Ltd. (Seongnam, Gyeonggi-do). As a test subject, 14 women aged 36 to 52 who were found to be suitable for the test were selected, and an appropriate amount of the cosmetic composition of the manufacturing example was applied twice a day (morning and evening) for 8 weeks and applied to the face.

Assessment is based on the Chromameter CR-400 (Muizzuddin N. et al., Use of a chromameter in assessing the efficacy of anti-irritants and tanning accelerators, Journal of the society of cosmetic chemists, 1990), which is widely used for skin color measurement in cosmetic research. 41: 369-378; Weethrall IL, et al., Skin color measurements in terms of CIELAB color space value, Journal of Investigative Dermatology, 1992; 99: 468-473). .

Chromameter CR-400 measured the L * value (the higher the L * value, the brighter the skin) as a parameter for skin brightness.The subject's subjective questionnaire evaluation evaluated the skin color of the test site by the 4-point grading method. It was evaluated whether the area of the pigmentation site was reduced and whether the skin texture at the test site was soft.

The statistical significance in the instrumental evaluation was confirmed by a paired t-test with a hypothetical mean difference of 5% (p <0.05).

The subjective questionnaire evaluation of the Chromameter CR-400 and the subjects was performed at the beginning, after 4 weeks, and after 8 weeks, and the L * value (mean value) measured using the Chromameter CR-400 and the degree of change (△ L * ₁ = 4 weeks) L * value after use-L * value before use, ΔL * ₂ = L * value after 8 weeks-L * value before use) in <Fig. 1> and <Fig. 2>, respectively. It is shown in Table 2.

Referring to <FIG. 1>, <FIG. 2> and <Table 2>, it can be seen that the skin whitening effect was generally used when the cosmetic composition including the pig placenta extract was used.

In particular, as a result of analyzing the change of L * value through the mechanical evaluation according to the usage period, after 4 weeks and after 8 weeks, both of them showed a statistically significant whitening effect (△ L * ₁ : 0.51 ㅁ 0.89). , ΔL * ₂ : 1.11 W1.02, p <0.05)

TABLE 3

Subject's Subjective Survey Evaluation Results

Experimental Example 2 Clinical Trial on Wrinkle Improvement Effect

Similarly, clinical evaluation was conducted by Ellid Co., Ltd. (Seongnam, Gyeonggi-do). As a test subject, 15 women with 33 ~ 46 years old eyes with wrinkles were selected for the test, and after applying the cosmetic composition of the preparation example twice a day (morning, evening) for 8 weeks, the appropriate amount was applied to the area of eye wrinkles. It was.

The evaluation was performed first (before use), after 4 weeks and after 8 weeks through replica analysis and image analysis using Visiometer (Skin-Visiometer SV 600, Courage & Khazaka, Germany).

The evaluation parameters by Visiometer are as follows (the smaller the value of R1 ~ R5 below, the better the skin wrinkles).

R1: Skin roughness (distance between the highest wrinkle point and the lowest wrinkle point)

R2: Maximum roughness (automatically divides wrinkles into 5 pieces to obtain R1 and then the highest R1 value among the divided wrinkles)

R3: Average roughness (average of R1 values obtained by dividing into five)

R4: Smoothness depth

R5: Arithmetic average roughness

For statistical significance, R1, R2, R3, R4, and R5, measured by Visiometer, were identified as paired t-test with a hypothetical mean difference of 5% (p <0.05).

 In Table 3 below, each of the R1, R2, R3, R4, and R5 values is represented as an average value of each subject, and each of ΔR1, ΔR2, ΔR3, ΔR4, and ΔR5 values is shown in FIG. 3 (4 weeks). Post-value before use) and FIG. 4 (value after 8 weeks—value before use).

TABLE 4

Image analysis results of the simulated version (mean value ㅁ standard deviation)

division R1 R2 R3 R4 R5 Before use 0.560 0.437 0.359 ㅁ 0.097 0.285 0.071 ㅁ 0.016 4 weeks later 0.508 0.388 ㅁ 0.062 0.254 W 0.041 0.186 0.063 ㅁ 0.018 8 weeks later 0.479 0.359 ㅁ 0.075 0.239 0.171 ㅁ 0.059 0.065 ㅁ 0.029

The results of <Table 3> and <FIG. 3> and <FIG. 4> are statistically compared with before and after use in the parameters R1, R2, and R3 after 4 weeks of use when using the cosmetic composition of the above-described preparation containing the pig placenta extract. Shows significant differences.

In addition, when the cosmetic composition containing the pig placenta extract was used during the experiment period, no skin abnormality reaction was observed, indicating that the cosmetic composition of the preparation example containing the pig placenta extract was recognized as safe.

1 is a graph showing the degree of change in L * value (mean value) over 4 weeks (ΔL * 1 = L * value after 4 weeks-L * value before use) measured using a Chromameter CR-400.

2 is a graph showing the ΔL * value (average value) (ΔL * ₂ = L * value after 8 weeks − L * value before use) over 8 weeks measured using a Chromameter CR-400.

3 is a graph showing the degree of change (value after 4 weeks before use) of R1, R2, R3, R4 and R5 values (mean value) over 4 weeks measured using a Visiometer.

4 is a graph showing the degree of change (value after 8 weeks-value before use) of R1, R2, R3, R4, and R5 values (average value) over 8 weeks measured using a Visiometer.

Claims (10)

Skin whitening composition comprising a pig placenta extract by ultrasound. The method of claim 1, Porcine placenta extract by the ultrasonic wave is a composition for skin whitening, characterized in that one of the extract of (a) to (f) (a) an extract obtained by crushing the placenta by adding water, ethanol or a mixed solvent thereof to lyophilized and powdered porcine placenta powder and radiating ultrasonic waves, (b) water, ethanol or a mixed solvent of lyophilized and powdered swine placenta powder, and the extract of the powder state in which the solvent is removed from the crushed product after crushing the placenta by radiating ultrasonic waves, (c) extract of the supernatant obtained by adding water, ethanol or a mixed solvent thereof to the lyophilized and powdered pig placenta powder, and radiating the placenta by radiating ultrasonic waves to centrifuge the crushed material to discard the precipitate; (d) powdered extract of the supernatant obtained by adding water, ethanol or a mixed solvent thereof to the lyophilized and powdered porcine placenta powder and radiating the placenta by radiating ultrasonic waves, and then centrifuging the crushed matter, (e) Water, ethanol or a mixed solvent thereof was added to the lyophilized and powdered pig placenta powder, and the ultrasonic place was crushed to break up the placenta, filtered to remove the extraction residue, and the filtrate was centrifuged to discard the precipitate. Extract of supernatant, and (f) Water, ethanol or a mixed solvent thereof was added to the lyophilized and powdered porcine placenta powder, the ultrasonic place was radiated to break the placenta, filtered to remove the extraction residue, and the filtrate was centrifuged to take a supernatant. Powdered product from which the solvent was removed from the supernatant. The method of claim 1, The composition is a composition for skin whitening, characterized in that the cosmetic composition. The method of claim 1, The composition is a composition for skin whitening, characterized in that the soap composition. The method of claim 1, The composition is a composition for skin whitening, characterized in that the pharmaceutical composition. Composition for improving skin wrinkles comprising a pig placenta extract by ultrasound. The method of claim 6, Pig placenta extract by the ultrasonic wave is a composition for improving skin wrinkles, characterized in that one of the extract of (a) to (d). (a) an extract obtained by crushing the placenta by adding water, ethanol or a mixed solvent thereof to lyophilized and powdered porcine placenta powder and radiating ultrasonic waves, (b) water, ethanol or a mixed solvent of lyophilized and powdered swine placenta powder, and the extract of the powder state in which the solvent is removed from the crushed product after crushing the placenta by radiating ultrasonic waves, (c) extract of the supernatant obtained by adding water, ethanol or a mixed solvent thereof to the lyophilized and powdered pig placenta powder, and radiating the placenta by radiating ultrasonic waves to centrifuge the crushed material to discard the precipitate; (d) powdered extract of the supernatant obtained by adding water, ethanol or a mixed solvent thereof to the lyophilized and powdered porcine placenta powder and radiating the placenta by radiating ultrasonic waves, and then centrifuging the crushed matter, (e) To the freeze-dried and powdered pig placenta powder, water, ethanol or a mixed solvent thereof was added and ultrasonic wave was crushed to break up the placenta, filtered to remove the extraction residue, and the filtrate was centrifuged to discard the precipitate. Extract of the obtained supernatant, and (f) Water, ethanol or a mixed solvent thereof was added to the lyophilized and powdered porcine placenta powder, the ultrasonic place was radiated to break the placenta, filtered to remove the extraction residue, and the filtrate was centrifuged to take a supernatant. Powdered product from which the solvent was removed from the supernatant. The method of claim 6, The composition is a composition for improving skin wrinkles, characterized in that the cosmetic composition. The method of claim 6, The composition is a composition for improving skin wrinkles, characterized in that the soap composition. The method of claim 6, The composition for improving skin wrinkles, characterized in that the pharmaceutical composition.

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