KR20090088492A - Development of sequence-based microsatellite markers in brassica rapa and construction of genetic map - Google Patents

Development of sequence-based microsatellite markers in brassica rapa and construction of genetic map Download PDF

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KR20090088492A
KR20090088492A KR1020080013786A KR20080013786A KR20090088492A KR 20090088492 A KR20090088492 A KR 20090088492A KR 1020080013786 A KR1020080013786 A KR 1020080013786A KR 20080013786 A KR20080013786 A KR 20080013786A KR 20090088492 A KR20090088492 A KR 20090088492A
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dna
brassica rapa
brassica
rapa
sequence
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진민아
김정선
박범석
권수진
김진아
김호일
문정환
이수인
임명호
양태진
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대한민국(관리부서:농촌진흥청)
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Abstract

A microsatellite marker which is improved from a genetic sequence of Brassica campestris ssp. is provided to use in maker assisted selection (MAS) and shorten the period of breeding crops. A primer pair of microsatellite marker which is improved from a sequence of Brassica campestris ssp. comprises two sequences among the sequence numbers 1-512. A genetic map of F3- pooled population is prepared by performing analysis of nucleic acid fingerprinting.

Description

배추 염기서열 정보로부터 개발된 미세부수체 분자 표지와 이를 이용해 작성된 유전자 지도{Development of sequence-based microsatellite markers in Brassica rapa and construction of genetic map}Development of sequence-based microsatellite markers in Brassica rapa and construction of genetic map

본 발명은 배추 염기서열 정보로부터 유전체 전반에 고르게 분포하는 미세부수체(microsatellite) 분자 표지를 개발하고 이를 이용하여 유전자지도를 작성한 것이다.The present invention develops a microsatellite molecular label that is evenly distributed throughout the genome from cabbage sequencing information and prepares a genetic map using the same.

분자 표지방법은 분자 육종시스템에서 유용 형질 탐색, 생물의 종 판별, 품종 분류·동정 및 집단 개체군의 유연관계 분석 등의 목적으로 널리 이용되고 있다. 전통 작물 육종은 유용 유전자원을 도입하기 위해 대량의 유전자원을 스크린해야 하고 형질을 정확하게 분석하기 위해 성숙기까지 작물을 길러야 하는 등 오랜 시간과 노력이 요구되는 어려움이 있다. 분자 표지를 이용한 작물 육종은 환경 변이에 영향을 받지 않고 어린 시기에 형질을 탐색할 수 있기 때문에 대량의 자원을 정확하고 빠르게 분석할 수 있는 장점이 있다. 또한, 분자 표지를 이용하여 작성된 유전자지도는 유용 농업형질과 관련된 유전자 및 양적 유전자좌의 위치를 탐색하는데 이용된다. 배추에서 개발된 분자표지는 배추뿐 아니라 양배추, 유채, 갓, 무 등 의 배추과 작물에도 이용될 수 있다.Molecular labeling methods are widely used in molecular breeding systems for the purpose of searching for useful traits, identifying species of organisms, classifying and identifying varieties, and analyzing the soft relationships of populations. Traditional crop breeding has a long time and effort that requires screening a large number of genetic resources to introduce useful genetic resources, and growing crops until maturity to accurately analyze traits. Crop breeding using molecular labels has the advantage of being able to analyze large quantities of resources accurately and quickly because they can search for traits at an early age without being affected by environmental variations. In addition, genetic maps created using molecular labels are used to explore the location of genes and quantitative loci associated with useful agricultural traits. Molecular labels developed from cabbage can be used not only for cabbage, but also for cabbage, crops such as cabbage, rapeseed, mustard and radish.

최근 몇십 년 동안 유전 변이를 탐색하여 분자표지로 활용하기 위한 다양한 방법이 개발되고 있다. 가장 먼저, 염색체 내 제한효소 인식부위의 변이에 의해 발생하는 염기서열 길이 차이를 이용한 RFLPs(restrictin Fragment Length Polymorphi는) 방법이 개발되었으나, 이 방법은 방사성 동위 원소를 사용해야 하는 번거로움이 있다. 이후 PCR 기법으로 분석이 가능한 RAPD(random amplified polymorphic DNA)와 AFLP(amplified fragment length polymorphism) 방법이 개발되어 한꺼번에 많은 다형성을 검출할 수 있지만 재현성이 낮은 단점이 있다.In recent decades, a variety of methods have been developed to explore genetic variations and use them as molecular markers. First of all, RFLPs (restrictin Fragment Length Polymorphi) have been developed using the sequence length difference caused by mutation of restriction sites in the chromosome. However, this method has the trouble of using radioisotopes. Since amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) methods have been developed, which can be analyzed by PCR, many polymorphisms can be detected at the same time.

SSR(simple sequence repeat), 즉 미세부수체 분자 표지는 염색체 내 단순 염기 서열의 반복 횟수 차이로 인한 변이에 기초하기 때문에 종마다 변이가 다양하고 염색체 내 고르게 분포하여 유용 형질 관련 분자표지로 개발될 가능성이 높다. 또한 재현성이 높아 AFLP나 RAPD의 단점을 극복할 수 있으며 PCR을 이용하여 간단하게 분석할 수 있다는 장점이 있다. 최근까지 배추과 내 유채, 양배추, 배추 등의 작물에서 개발된 미세부수체 표지 인자들은 작은 라이브러리 제작을 통해 이루어졌으며, 이는 클론의 중복성과 키메라 현상으로 인해 대량으로 표지 인자를 개발하는데 어려움이 있었다. 본 연구에서는 배추 유전체 해독연구에서 생산된 거대단편의 염기서열 정보를 이용하여 별도의 라이브러리를 제작하지 않고 유전체 내의 표지 인자를 직접 개발하는 방법을 이용하였다. 각각의 미세부수체 표지 인자들이 염기서열이 완전 해독된 애기장대와 상동성 비교를 통해 선발되었기 때문에 표지 인자 개발을 통해 이들의 위치가 밝혀지면 배추 유전체 완전 해독을 위한 종자 거대 단 편으로 제공됨과 동시에 애기장대와 배추의 비교연구를 위한 정보를 제공할 수 있다. 애기장대와 배추는 진화적으로 매우 가까워 유전자 수준에서 유사도가 89%로 높기 때문에 이들의 비교 유전연구를 통하여 이미 다양한 연구가 진행된 애기장대의 유전자 기능을 배추에 적용할 수 있다.Simple sequence repeat (SSR), or microsatellite molecule markers, is based on variations due to differences in the number of repeats of simple sequences within the chromosome. This is high. In addition, the high reproducibility can overcome the shortcomings of AFLP and RAPD, and has the advantage of simple analysis using PCR. Until recently, microsatellite markers developed in crops such as Chinese cabbage, rapeseed, cabbage, and Chinese cabbage were made through the production of small libraries, which made it difficult to develop markers in large quantities due to clonal redundancy and chimera. In this study, we used the method of directly developing the labeling factor in the genome without making a separate library by using the nucleotide sequence information of the large fragments produced in the cabbage genome deciphering study. Since each of the microsatellite markers was selected by homologous comparison with the fully deciphered Arabidopsis larvae, once they were identified through the development of the markers, they were provided as seed giant fragments for complete detoxification of the cabbage genome. Provide information for comparative studies of Arabidopsis and cabbage. Arabidopsis and cabbage are evolutionarily close and have 89% similarity at the gene level, so they can apply the gene function of Arabidopsis to cabbage, which has already been studied through comparative genetic studies.

2008년 현재 우리나라의 십자화과 채소 작물은 배추와 무 등 대표적인 작물을 중심으로 약 330억원 규모의 종자시장을 형성하고 있으며, 연간 약 1조 340억 원 규모의 십자화과 채소가 생산되고 있어 전체 채소 생산량의 약 14%를 차지하고 있다. 십자화과 채소 육종 연구는 전통적으로 병해충 저항성, 내동성, 내서성 등 생산량 증대를 목표로 하며 최근 소비자의 기호에 맞추어 유용 성분 및 항암 약리성분을 함유한 고품질 품종 개발 등 그 연구 수요 분야가 다양해지고 있다. 국내에서는 늘어나는 수요에 비해 그 기술수준은 전통적인 방법에서 벗어나지 못하고 있지만 일본, 캐나다, 영국 등에서는 고유한 분자 육종 시스템을 구축하여 지적재산권을 확보해 나가고 있다. 최근 국내에서도 배추 유전체 해독 사업이 진행되면서 대량의 유전정보가 분석되었고 이를 통해 배추뿐만 아니라 십자화과 채소 육종 분야에서 분자 육종 시스템을 위한 인프라가 구축되어 국가 경쟁력 제고에 기여할 수 있게 되었다.As of 2008, Korea's cruciferous vegetable crops form a seed market of about 33 billion won, mainly representative crops such as Chinese cabbage and radish. 14%. Cruciferous vegetable breeding research has traditionally aimed at increasing production, such as pest resistance, tolerance, and resistance, and has recently diversified its research demand fields such as developing high-quality varieties containing useful ingredients and anti-cancer pharmacological ingredients according to consumers' preferences. Compared to the growing demand in Korea, the technology level has not escaped from the traditional method, but Japan, Canada, and the United Kingdom have established their own molecular breeding system to secure intellectual property rights. Recently, a large amount of genetic information was analyzed in the domestic cabbage genome detoxification project, and through this, infrastructure for molecular breeding system was established not only for cabbage but also for cruciferous and vegetable breeding, thus contributing to national competitiveness.

본 발명은 통해 배추 유전체 정보를 기반으로 개발된 대량의 미세부수체 분자 표지를 공급하고 이를 활용하여 작성된 유전자지도를 통해 작물 육종, 품종 판별, 유용 유전자 탐색 등에 기여하고자 한다.The present invention is to supply a large number of microsatellite molecule markers developed based on the cabbage genome information through, and to contribute to crop breeding, breed identification, useful gene search through genetic map created using this.

본 발명은 배추 유전체 염기서열로부터 개발된 서열번호 1 내지 514의 미세 부수체 분자표지의 프라이머 쌍을 제공한다.The present invention provides primer pairs of the microacoustic molecular labels of SEQ ID NOs: 1 to 514 developed from Chinese cabbage genome sequences.

또한 본 발명은 상기 미세 부수체 분자표지를 이용하여 장원 F3-풀드 집단에 대해 작성된 유전자지도를 제공한다.The present invention also provides a genetic map prepared for the mandrel F3-pulled population using the microacoustic molecular label.

본 발명은 배추 유전체 해독 연구를 통해 생산된 거대단편인자(BAC 클론)의 염기서열 정보를 기반으로 미세부수체를 예측하고 이들의 반복 횟수 차이에 의한 다형성을 이용하여 분자표지로 개발한 것이다.The present invention was developed as a molecular marker using polymorphism by predicting the microsatellite based on the nucleotide sequence information of the macrofragment factor (BAC clone) produced through the Chinese cabbage genome deciphering research.

상이 목적을 위해 배추(Brassica rapa) 유전체 서열정보로부터 인실리코(in silico) 상에서 미세부수체를 탐색하고 일부에 대해 프라이머를 설계하여 유전지도 작성 집단의 양친에 대해 다양성을 확인하는 과정을 포함한다.Chinese cabbage (Brassica for different purposes rapa ) to search for microsatellites on in silico from genomic sequence information and design primers for some to identify diversity for the parents of the gene mapping population.

또한 상기 다양성이 확인되는 분자 표지를 이용하여 장원 F3p 집단에 대해 작성한 유전자 지도를 포함한다.Also included is a genetic map created for the manor F3p population using the molecular label for which the diversity is identified.

본 발명의 미세 부수체 분자표지와 유전자 지도 역시 배추 유전체 해독 연구를 통해 생산된 정보를 기반으로 개발되었으며 이는 배추 분자 육종 시스템(Maker assisted selection: MAS)에 활용되어 작물 육종 연한을 획기적으로 단축시키고 유용한 유전자 및 형질을 탐색하는데 기여할 수 있다. 최근 종자 산업법의 일환으로 품종 보호를 통한 지적재산권이 인정되면서 분자 표지 시스템의 필요성이 대두되고 잇는데, 본 발명을 통해 개발된 분자표지를 이용할 경우 배추 품종을 구분하기 위한 지표로 활용될 수 있다.The microacoustic molecular markers and genetic maps of the present invention were also developed based on information produced through cabbage genome detoxification studies, which have been utilized in the maker assisted selection (MAS) to dramatically shorten crop breeding age and provide useful information. Contribute to the search for genes and traits. Recently, as part of the seed industry law, the intellectual property rights through the protection of varieties are recognized, the necessity of a molecular labeling system is emerging. When the molecular label developed through the present invention is used, it can be used as an index for distinguishing cabbage varieties.

이하 실시예에서 본 발명에 대해 상세히 설명한다.Hereinafter, the present invention will be described in detail.

[실시예 1] 미세부수체 분석 및 프라이머 설계Example 1 Microacoustic Analysis and Primer Design

배추 염기서열에 기반하는 분자표지 개발을 위해 배추 거대단편인자의 파지(phage) Ⅰ 혹은 파지(phage) Ⅱ 단계의 컨디그의 염기서열 내에 포함되어 있는 미세부수체를 추출해야 한다. 이를 위해 평균 100kb의 거대 단편 인자 600여개의 염기서열을 에스에스알아이티(SSRIT; http://www.gramene.org/db/searches /ssrtool), 에스피유티엔아이케이(SPUTNIK; http://www.abajian.co/sputnik), 리피트마스커(RepeatMasker; http://www.repeatmasker.org/cgi-bin/WEBRepeatMasker), 웹트롤(Webtroll; http://wsmartins.net/webtroll/troll.html), 텐덤 리피트 파인더(Tandem Repeat Finder; http://tandem.bu.edu /trf/trf.html). 아르고스(ARGOS; http://genebank.rga.go.kr/argos)의 프로그램을 통해 반복염기서열을 분석하였다. 프로그램 별 중복성이 높고 신뢰도가 큰 미세부수체들을 선별하고 이 중에서 반복의 길이가 긴 미세부수체를 중심으로 배추 거대단편인자 별 평균 3개 혹은 4개의 프라이머가 설계되었다. 에스에스알아이티(SSRIT), 에스피유티엔아이케(SPUTNIK), 리피트마스커(RepeatMasker), 텐덤 리피트 파인더(Tandem Repeat Finder)의 프로그램은 반복염기서열 탐색만 가능하기 때문에 선정된 미세부수체에 대해 프라이머3(Primer3; http://frodo.wi.mit.edu/)라는 프라이머 설계 전용 프로그램을 이용하여 프라이머를 설계하는 2단계의 작업을 수행하였다. 웹트롤(Webtroll)이나 아르고스(ARGOS)의 경우 반복염기서열 탐색과 프라이머 설계가 자동으로 연결되어 있어 한번에 분석이 가능하다.In order to develop molecular markers based on cabbage nucleotide sequences, it is necessary to extract the microsatellites contained in the phage I or phage Ⅱ sequence of the Chinese cabbage macrofragment factor. To this end, an average of 600 nucleotide sequences of 100 kb fragment fragments were used for SSRIT (http://www.gramene.org/db/searches / ssrtool) and SPUTNIK (http: // www). .abajian.co / sputnik), RepeatMasker; http://www.repeatmasker.org/cgi-bin/WEBRepeatMasker, Webtroll; http://wsmartins.net/webtroll/troll.html Tandem Repeat Finder (http://tandem.bu.edu /trf/trf.html). The repeat base sequence was analyzed through the program of Argos (ARGOS; http://genebank.rga.go.kr/argos). In each program, the high redundancy and high reliability of the microsatellites were selected. Among them, the average of three or four primers per cabbage macrofragment factor was designed around the microsatellites with a long repetition length. The programs of SSRIT, SPUTNIK, RepeatMasker, and Tandem Repeat Finder can only search for repeat base sequences. Using primer design-only program (Primer3; http://frodo.wi.mit.edu/), two steps of primer design were performed. In the case of Webtroll or ARGOS, repetitive sequence search and primer design are automatically linked for analysis at once.

[실시예 2] 프라이머의 다형성 분석Example 2 Polymorphism Analysis of Primer

설계된 프라이머는 배추 품종인 장원의 양친에 대해 다형성이 검토되었다. PCR 반응을 위한 조성은 총 29㎕로 하여 장원 양친 및 분리집단으로부터 분리한 게놈 DNA(25ng), dNTPs(각 200mM), 타크 폴리머라아제(Taq polymerase; 1 unit, Biostream 사), 10배 완충액 및 설계된 프라이머 한 쌍(각 10pmol)으로 구성되었고 PCR 반응은 써모 사이클러(Thermo cycler) 기기를 이용하여 94℃에서 5분간 열처리하고 95℃에서 30초 변성단계, 57~61℃에서 30초 프라이머 연결단계, 72℃에서 1분 DNA 합성단계로 총 35회 반복하여 PCR 증폭을 실시하였으며 상기의 PCR 반응의 결과물은 2.5% 아가로스 겔이나 5% 폴리아크릴아마이드 겔에서 전기영동하여 증폭산물과 양친간 다형성을 확인하였다. 상기 방법으로 확인된 분자표지 256쌍의 정보를 표 1에 정리하였다.The designed primers were examined for polymorphism against the parents of the cabbage variety Jangwon. The composition for the PCR reaction was 29 μl in total, and genomic DNA (25 ng), dNTPs (200 mM each), Taq polymerase (1 unit, Biostream), 10-fold buffer and It was composed of a pair of primers designed (10 pmol each) and PCR reaction was heat treated at 94 ℃ for 5 minutes using a thermo cycler device, 30 seconds denaturation step at 95 ℃, 30 seconds primer connection step at 57 ~ 61 ℃ PCR amplification was repeated a total of 35 times at 72 ° C. for 1 minute DNA synthesis step. The result of the PCR reaction was electrophoresed on 2.5% agarose gel or 5% polyacrylamide gel to increase polymorphism between amplified product and parent. Confirmed. The information of 256 pairs of molecular labels identified by the above method is summarized in Table 1.

[표 1] 배추 장원 양친에서 다형성을 보이는 256쌍 미세부수체 분자 표지의 유전정보[Table 1] Genetic information of 256 pairs of microsatellite molecules with polymorphism in Chinese cabbage

Figure 112008011365390-PAT00001
Figure 112008011365390-PAT00001

Figure 112008011365390-PAT00002
Figure 112008011365390-PAT00002

Figure 112008011365390-PAT00003
Figure 112008011365390-PAT00003

Figure 112008011365390-PAT00004
Figure 112008011365390-PAT00004

Figure 112008011365390-PAT00005
Figure 112008011365390-PAT00005

Figure 112008011365390-PAT00006
Figure 112008011365390-PAT00006

Figure 112008011365390-PAT00007
Figure 112008011365390-PAT00007

Figure 112008011365390-PAT00008
Figure 112008011365390-PAT00008

Figure 112008011365390-PAT00009
Figure 112008011365390-PAT00010
Figure 112008011365390-PAT00009
Figure 112008011365390-PAT00010

Figure 112008011365390-PAT00011
Figure 112008011365390-PAT00011

[실시예 3] 11개 미세 부수체 프라이머를 이용한 배추 계통의 핵산 지문 분석Example 3 Nucleic Acid Fingerprint Analysis of Cabbage Lines Using 11 Microacoustic Primers

도 1은 KBrH091P11, KBrH033J07, KBrH087J23, KBrH055N18, KBrH099I08, KBrH066J16, KBrS022B15의 7개 배추 거대단편 인자에서 1개 내지 2개 설계된 총 11개의 프라이머를 4종류의 배추(장원 양친 각 1개, 지부, 권심)를 대상으로 PCR 증폭을 하고, 5% 폴리아크릴아마이드 젤에서 PCR 증폭산물의 다형성을 확인한 결과를 나타낸 그림이다. 11개 중에서 H099I08-115를 제외하고 10개의 프라이머가 예상 증폭 크기대로 증폭되었다. 장원 양친(1과 2)에서 H091P11-32, H091P11-82, H033J07-108, S002B15-30, H099I08-107, H099I08-130 5개의 프라이머가 다형성을 보였고 지부와 권심(3과 4)에서는 H091P11-82, S002B15-30, H099I08-107, H099I08-130의 4개 프라이머가 다형성을 보였다. 장원 양친과 지부/권심 계통에서 다형성을 찾을 수 없는 프라이머( H033J07-18, H087J23-46, H066J16-54, H099I08-115)도 11개 중에 4개 존재한다. 1 shows four types of Chinese cabbage (one with each of the two calves, one with each of the two rounds) of 11 primers designed from one to two of the Chinese cabbage giant fragments of KBrH091P11, KBrH033J07, KBrH087J23, KBrH055N18, KBrH099I08, KBrH066J16, and KBrS022B15. PCR amplification was carried out in the 5% polyacrylamide gel PCR amplification products confirmed the results of the results. Ten of the 11 primers were amplified to the expected amplification size except H099I08-115. H091P11-32, H091P11-82, H033J07-108, S002B15-30, H099I08-107, H099I08-130 in the parents' parents (1 and 2) showed polymorphism and H091P11-82 in the branches and windings (3 and 4). Four primers, S002B15-30, H099I08-107 and H099I08-130, showed polymorphism. Four out of 11 primers (H033J07-18, H087J23-46, H066J16-54, H099I08-115) that do not find polymorphisms in the manor parent and branch / central lineages are also present.

[실시예 4] 분자 표지를 이용한 배추 유전 집단 분석과 유전자 지도 작성Example 4 Analysis of Chinese Cabbage Genetic Population and Genetic Mapping Using Molecular Labels

도 2는 양친 핵산지문 분석 결과 장원 양친에서 다형성을 보이는 KS10320 분자 표지를 이용하여 장원 F3-pooled 집단 94 계통에 대해 PCR하고 그 산물을 5% 폴리아크릴아마이드 젤에서 전기영동을 실시한 결과이다. 도 3은 KS11360 분자 표지를 이용하여 장원 F3-풀드(pooled) 집단 94 계통에 대해 PCR하고 그 산물을 1% 아가로스 젤에서 전기영동을 실시한 결과이다. KS10320는 305bp, KS11360는 196bp의 예상 크기대로 증폭되었으며 각 분리집단의 1/4은 모본, 1/4는 부본, 1/2은 양친 모두의 대립인자를 가진 것을 확인할 수 있다. FIG. 2 shows the results of PCR using the KS10320 molecular label showing polymorphisms in the parental parentage analysis of the parent nucleic acid fingerprints, and PCR of the 94 family of the F3-pooled population 94. The product was subjected to electrophoresis on a 5% polyacrylamide gel. FIG. 3 shows the results of PCR of the manor F3-pooled population 94 strains using KS11360 molecular labeling and electrophoresis of the product on 1% agarose gel. KS10320 was amplified to 305bp and KS11360 to 196bp, and one-quarter of each isolate had a parent, one-quarter with a copy, and half with alleles of both parents.

표 1에서 나열된 256쌍의 미세 부수체(microsatellte) 분자 표지들에 대해서 모두 장원 F3-풀드(pooled) 집단에 대한 핵산지문 분석이 이루어졌다. 분리집단에서 모본의 대립인자를 가진 경우를 A, 부본의 것을 B, 둘 다 가진 것을 H라고 핵산지물 분석 결과를 해독한 다음 조인맵(JoniMap) 프로그램을 통해 이들의 연관분석을 수행하였다. 연관 분석은 LOD 3.0 수준에서 이루어졌으며 그 결과 10개의 연관군이 생성되었고 그 크기는 1078 cM이다. 연관군 각각의 크기와 마커의 개수는 표 2와 같으며 연관군의 모식도는 도 4와 같다.Nucleic acid fingerprint analyzes were performed for all of the manor F3-pooled populations for the 256 pairs of microsatellte molecular markers listed in Table 1. In the isolated group, the nucleic acid markers were decoded as A, the B of the copy, and the H of the copy, and the association analysis was performed through the JoinMap program. Association analysis was performed at the LOD 3.0 level, resulting in 10 association groups and 1078 cM in size. The size of each associated group and the number of markers are shown in Table 2, and the schematic diagram of the associated group is shown in FIG. 4.

[표 2] 연관군 별 미세부수체 분자 표지의 개수와 길이[Table 2] Number and length of microsatellite molecule markers by related group

연관군Associative group 1One 22 33 44 55 66 77 88 99 1010 전체all 분자표지 수(개)Molecular Labels () 2323 2727 4444 1010 2121 2727 1717 2121 5151 1515 256256 길이(cM)Length (cM) 105.1105.1 88.888.8 135.5135.5 59.459.4 152.0152.0 132.2132.2 101.2101.2 78.978.9 169.9169.9 55.155.1 1078.01078.0

도 1은 미세 부수체(Microsatellte) 프라이머의 배추 4개 계통에 대한 다형성 탐색한 그림이다.1 is a diagram illustrating the polymorphism of four cabbage strains of a microsatellte primer.

도 2는 5% 폴리아크릴아마이드 겔에서 KS10320 분자 표지의 장원 F3p 집단에 대한 핵산지문을 분석한 것이다.Figure 2 shows the analysis of nucleic acid fingerprints for the F3p population of the KS10320 molecular label in 5% polyacrylamide gels.

도 3은 1% 아가로스 겔에서 KS11360 분자 표지의 장원 F3p 집단에 대한 핵산지문을 분석한 것이다.Figure 3 shows the analysis of nucleic acid fingerprints for the ancestor F3p population of the KS11360 molecular label in a 1% agarose gel.

도 4는 256쌍 미세부수체(microsatellte) 분자 표지로 작성된 장원 F3-풀드(pooled) 집단의 유전자지도이다.FIG. 4 is a genetic map of the manor F3-pooled population prepared with 256 pairs of microsatellte molecular labels.

<110> Korea <120> Development of sequence-based microsatellite markers in Brassica rapa and construction of genetic map <160> 512 <170> KopatentIn 1.71 <210> 1 <211> 20 <212> DNA <213> Brassica rapa <400> 1 ttctttggag atggtgtggt 20 <210> 2 <211> 20 <212> DNA <213> Brassica rapa <400> 2 caatcttgtg gtgagggaag 20 <210> 3 <211> 20 <212> DNA <213> Brassica rapa <400> 3 tctccatctc tcttccatcg 20 <210> 4 <211> 20 <212> DNA <213> Brassica rapa <400> 4 gtcctgcctg gtttgaataa 20 <210> 5 <211> 18 <212> DNA <213> Brassica rapa <400> 5 gttttaaatg ccgcgttg 18 <210> 6 <211> 19 <212> DNA <213> Brassica rapa <400> 6 gggatcaaga gatgtggga 19 <210> 7 <211> 21 <212> DNA <213> Brassica rapa <400> 7 tgatgaaaaa tgcatcacaa c 21 <210> 8 <211> 20 <212> DNA <213> Brassica rapa <400> 8 ttcatgtgga agcatttgag 20 <210> 9 <211> 20 <212> DNA <213> Brassica rapa <400> 9 cgggtcgtat tctctaacca 20 <210> 10 <211> 20 <212> DNA <213> Brassica rapa <400> 10 actcgccttc tccaactttt 20 <210> 11 <211> 20 <212> DNA <213> Brassica rapa <400> 11 gacagtggtt gaaaaagcca 20 <210> 12 <211> 20 <212> DNA <213> Brassica rapa <400> 12 cattcagtga ggtttcacca 20 <210> 13 <211> 20 <212> DNA <213> Brassica rapa <400> 13 gtcaacaacc tccgcgatag 20 <210> 14 <211> 20 <212> DNA <213> Brassica rapa <400> 14 agatcactca ccttcccggt 20 <210> 15 <211> 21 <212> DNA <213> Brassica rapa <400> 15 gaccttcggt tcagggtatg g 21 <210> 16 <211> 21 <212> DNA <213> Brassica rapa <400> 16 ctgaacggtc aatttgtttg g 21 <210> 17 <211> 20 <212> DNA <213> Brassica rapa <400> 17 tcaagcgcaa caaacattgg 20 <210> 18 <211> 24 <212> DNA <213> Brassica rapa <400> 18 tggtgttagg gtttaaggtt gtgg 24 <210> 19 <211> 20 <212> DNA <213> Brassica rapa <400> 19 caaaaagagc gttacctcca 20 <210> 20 <211> 20 <212> DNA <213> Brassica rapa <400> 20 gatgaagctc tgaagaccga 20 <210> 21 <211> 20 <212> DNA <213> Brassica rapa <400> 21 acctaccaac cccaaaaagg 20 <210> 22 <211> 20 <212> DNA <213> Brassica rapa <400> 22 gaagctttcg atgcggttta 20 <210> 23 <211> 21 <212> DNA <213> Brassica rapa <400> 23 ttaccgtgga tattggatga a 21 <210> 24 <211> 20 <212> DNA <213> Brassica rapa <400> 24 gccaggctaa gtgatgattc 20 <210> 25 <211> 22 <212> DNA <213> Brassica rapa <400> 25 ggatatctga tttgacccgt at 22 <210> 26 <211> 22 <212> DNA <213> Brassica rapa <400> 26 aaccgaaaac tccagataat ca 22 <210> 27 <211> 20 <212> DNA <213> Brassica rapa <400> 27 tgtgcttgcg ttttaaagga 20 <210> 28 <211> 20 <212> DNA <213> Brassica rapa <400> 28 gcaaaaccca caggtcagat 20 <210> 29 <211> 22 <212> DNA <213> Brassica rapa <400> 29 agaccagtcg gatctagagg at 22 <210> 30 <211> 22 <212> DNA <213> Brassica rapa <400> 30 caaccacatg agattggttt ag 22 <210> 31 <211> 22 <212> DNA <213> Brassica rapa <400> 31 ttctccccat cctctcatct ta 22 <210> 32 <211> 22 <212> DNA <213> Brassica rapa <400> 32 acccacaacc aacaaaatct tc 22 <210> 33 <211> 20 <212> DNA <213> Brassica rapa <400> 33 cggatgagag gatttacacg 20 <210> 34 <211> 20 <212> DNA <213> Brassica rapa <400> 34 cctcctggag cttatccatt 20 <210> 35 <211> 26 <212> DNA <213> Brassica rapa <400> 35 tcttcaagtc aaaatactca cattca 26 <210> 36 <211> 27 <212> DNA <213> Brassica rapa <400> 36 aaacgacaaa tacatatgac agtttta 27 <210> 37 <211> 20 <212> DNA <213> Brassica rapa <400> 37 ctatctcaga ccagcggaaa 20 <210> 38 <211> 20 <212> DNA <213> Brassica rapa <400> 38 ttgcataatc gtggaacgta 20 <210> 39 <211> 20 <212> DNA <213> Brassica rapa <400> 39 gagtgggatt ctcatcatgg 20 <210> 40 <211> 20 <212> DNA <213> Brassica rapa <400> 40 taccgtcttg gctcttgagt 20 <210> 41 <211> 25 <212> DNA <213> Brassica rapa <400> 41 tctttattca atttgctcac attcc 25 <210> 42 <211> 20 <212> DNA <213> Brassica rapa <400> 42 ttcgtggttt cgcggattat 20 <210> 43 <211> 20 <212> DNA <213> Brassica rapa <400> 43 tatcaagtgg ggacgaatgg 20 <210> 44 <211> 20 <212> DNA <213> Brassica rapa <400> 44 aaggaagaag ggtggaatcg 20 <210> 45 <211> 20 <212> DNA <213> Brassica rapa <400> 45 catcaacgtg gcatagtctg 20 <210> 46 <211> 20 <212> DNA <213> Brassica rapa <400> 46 caaatttctg tgttgcgttg 20 <210> 47 <211> 24 <212> DNA <213> Brassica rapa <400> 47 gcgccaatta taaatttgat tttc 24 <210> 48 <211> 20 <212> DNA <213> Brassica rapa <400> 48 tcctcctgaa cctggtcttg 20 <210> 49 <211> 23 <212> DNA <213> Brassica rapa <400> 49 ttgtgcacca atctattaca aaa 23 <210> 50 <211> 23 <212> DNA <213> Brassica rapa <400> 50 tgaagctgtt cttacaaaac aaa 23 <210> 51 <211> 22 <212> DNA <213> Brassica rapa <400> 51 tatagcctta gtggtagcga gg 22 <210> 52 <211> 22 <212> DNA <213> Brassica rapa <400> 52 ttttacccca agaatgtttg tc 22 <210> 53 <211> 20 <212> DNA <213> Brassica rapa <400> 53 aggggagatg gatctggtgt 20 <210> 54 <211> 20 <212> DNA <213> Brassica rapa <400> 54 tgagactcgc taggtttgcc 20 <210> 55 <211> 18 <212> DNA <213> Brassica rapa <400> 55 ggatcctccc atagctcg 18 <210> 56 <211> 18 <212> DNA <213> Brassica rapa <400> 56 cagtcgttgc gggataga 18 <210> 57 <211> 20 <212> DNA <213> Brassica rapa <400> 57 cgatttcgaa gtagccttca 20 <210> 58 <211> 20 <212> DNA <213> Brassica rapa <400> 58 tcaagaagca aatgaaaggg 20 <210> 59 <211> 18 <212> DNA <213> Brassica rapa <400> 59 tacgattgtg tgcatggc 18 <210> 60 <211> 20 <212> DNA <213> Brassica rapa <400> 60 aaccatctag accaccacga 20 <210> 61 <211> 19 <212> DNA <213> Brassica rapa <400> 61 tcacattatt ttgtcgccg 19 <210> 62 <211> 20 <212> DNA <213> Brassica rapa <400> 62 tcacaacatt tgaccgtttg 20 <210> 63 <211> 20 <212> DNA <213> Brassica rapa <400> 63 caccatgtga caaaacgaaa 20 <210> 64 <211> 21 <212> DNA <213> Brassica rapa <400> 64 ggacaaggat aatgatctcg g 21 <210> 65 <211> 20 <212> DNA <213> Brassica rapa <400> 65 tgatatcgaa aacaaatgcg 20 <210> 66 <211> 21 <212> DNA <213> Brassica rapa <400> 66 tgtctgcaac atcagtttca a 21 <210> 67 <211> 20 <212> DNA <213> Brassica rapa <400> 67 tgtttgaatc gtccattgtg 20 <210> 68 <211> 22 <212> DNA <213> Brassica rapa <400> 68 tggacgtaca tgcctactaa ca 22 <210> 69 <211> 20 <212> DNA <213> Brassica rapa <400> 69 ttgtcctctt ttcgaacctg 20 <210> 70 <211> 21 <212> DNA <213> Brassica rapa <400> 70 ttcctcgacc aattagtttc a 21 <210> 71 <211> 27 <212> DNA <213> Brassica rapa <400> 71 gctaaaggtt tagtccaaat aggattc 27 <210> 72 <211> 20 <212> DNA <213> Brassica rapa <400> 72 gcaaaatgat gccccataaa 20 <210> 73 <211> 20 <212> DNA <213> Brassica rapa <400> 73 tcgtttaccg gtcgtacgtt 20 <210> 74 <211> 20 <212> DNA <213> Brassica rapa <400> 74 gcctgagaga ccgtgagaga 20 <210> 75 <211> 23 <212> DNA <213> Brassica rapa <400> 75 agcacagtca aaactcagaa tga 23 <210> 76 <211> 20 <212> DNA <213> Brassica rapa <400> 76 aggcatgcgc tctctatctc 20 <210> 77 <211> 22 <212> DNA <213> Brassica rapa <400> 77 aaaagttggt cattcttctg ga 22 <210> 78 <211> 22 <212> DNA <213> Brassica rapa <400> 78 gtttatggtt acgggtggat aa 22 <210> 79 <211> 20 <212> DNA <213> Brassica rapa <400> 79 gacgacagct tcgaaagaga 20 <210> 80 <211> 20 <212> DNA <213> Brassica rapa <400> 80 tgagcattgt tttggtgatg 20 <210> 81 <211> 22 <212> DNA <213> Brassica rapa <400> 81 cgtatccatg gccttgaatt tt 22 <210> 82 <211> 20 <212> DNA <213> Brassica rapa <400> 82 ggcgagaacc ttgatgatcc 20 <210> 83 <211> 20 <212> DNA <213> Brassica rapa <400> 83 taatgccaca tcagcagaaa 20 <210> 84 <211> 20 <212> DNA <213> Brassica rapa <400> 84 acagcggtga aatgaactgt 20 <210> 85 <211> 18 <212> DNA <213> Brassica rapa <400> 85 gccgctggac acctaaat 18 <210> 86 <211> 19 <212> DNA <213> Brassica rapa <400> 86 catggtacgt cggttttga 19 <210> 87 <211> 21 <212> DNA <213> Brassica rapa <400> 87 cagtgaagtt caaccgcagt a 21 <210> 88 <211> 25 <212> DNA <213> Brassica rapa <400> 88 catgagtgaa cataaaacag tgaaa 25 <210> 89 <211> 22 <212> DNA <213> Brassica rapa <400> 89 catcactttt tcaggtggat tt 22 <210> 90 <211> 22 <212> DNA <213> Brassica rapa <400> 90 ctttctctct acctggtgat gg 22 <210> 91 <211> 19 <212> DNA <213> Brassica rapa <400> 91 tcctcgtgct tgtgaacag 19 <210> 92 <211> 19 <212> DNA <213> Brassica rapa <400> 92 tctgtttgtt ccgcagatg 19 <210> 93 <211> 22 <212> DNA <213> Brassica rapa <400> 93 tgttaccaca ggaaccgttc aa 22 <210> 94 <211> 20 <212> DNA <213> Brassica rapa <400> 94 cttgggcaca ccatcatctg 20 <210> 95 <211> 20 <212> DNA <213> Brassica rapa <400> 95 gctcatcctt ctcatgcagt 20 <210> 96 <211> 22 <212> DNA <213> Brassica rapa <400> 96 tggtattcgt acgttaaaca cg 22 <210> 97 <211> 20 <212> DNA <213> Brassica rapa <400> 97 ccctttccgt aaatccactt 20 <210> 98 <211> 20 <212> DNA <213> Brassica rapa <400> 98 tttgcggttg aaactcattt 20 <210> 99 <211> 20 <212> DNA <213> Brassica rapa <400> 99 atgtcatcaa gaccatcccg 20 <210> 100 <211> 20 <212> DNA <213> Brassica rapa <400> 100 gcatggcttg gtttcagaac 20 <210> 101 <211> 24 <212> DNA <213> Brassica rapa <400> 101 tggcattgac agagctagta agag 24 <210> 102 <211> 26 <212> DNA <213> Brassica rapa <400> 102 tttatttagt ttcataccct cgcagt 26 <210> 103 <211> 20 <212> DNA <213> Brassica rapa <400> 103 ccatgactcc attgtcgatt 20 <210> 104 <211> 20 <212> DNA <213> Brassica rapa <400> 104 cctgatcttc atgctggttc 20 <210> 105 <211> 20 <212> DNA <213> Brassica rapa <400> 105 ccccacccct taagtgctat 20 <210> 106 <211> 20 <212> DNA <213> Brassica rapa <400> 106 agagaggaag aaggagcgga 20 <210> 107 <211> 20 <212> DNA <213> Brassica rapa <400> 107 acaaaatttc tggccaatca 20 <210> 108 <211> 20 <212> DNA <213> Brassica rapa <400> 108 acggtggtgg ttagatgaga 20 <210> 109 <211> 20 <212> DNA <213> Brassica rapa <400> 109 tcgaggtggt tacaatccaa 20 <210> 110 <211> 20 <212> DNA <213> Brassica rapa <400> 110 caatgcggat ctacctctca 20 <210> 111 <211> 24 <212> DNA <213> Brassica rapa <400> 111 tcaagcatgt ccttaaaact ctga 24 <210> 112 <211> 20 <212> DNA <213> Brassica rapa <400> 112 gcgttcacgt ttcccatatc 20 <210> 113 <211> 22 <212> DNA <213> Brassica rapa <400> 113 gagaagaatt ttcccactat gc 22 <210> 114 <211> 27 <212> DNA <213> Brassica rapa <400> 114 gattttattg acaatgtata tttgctg 27 <210> 115 <211> 21 <212> DNA <213> Brassica rapa <400> 115 tgaaccctcg aaactgaaag a 21 <210> 116 <211> 20 <212> DNA <213> Brassica rapa <400> 116 ggacgttaac gaaggcaaaa 20 <210> 117 <211> 24 <212> DNA <213> Brassica rapa <400> 117 tttttgggtt tcttctcaaa atgc 24 <210> 118 <211> 20 <212> DNA <213> Brassica rapa <400> 118 actccagcga atttggcttt 20 <210> 119 <211> 20 <212> DNA <213> Brassica rapa <400> 119 tttgacatcg tgcaatgcta 20 <210> 120 <211> 20 <212> DNA <213> Brassica rapa <400> 120 ttgggctggt cctgaagata 20 <210> 121 <211> 22 <212> DNA <213> Brassica rapa <400> 121 actctttccc tctgcttcaa at 22 <210> 122 <211> 22 <212> DNA <213> Brassica rapa <400> 122 ttcaaaacga gagaggttag ga 22 <210> 123 <211> 22 <212> DNA <213> Brassica rapa <400> 123 ttcttgacca aaagaatcat gg 22 <210> 124 <211> 20 <212> DNA <213> Brassica rapa <400> 124 ctaacacggg gaaaagcaga 20 <210> 125 <211> 21 <212> DNA <213> Brassica rapa <400> 125 ccaaatgcag cagcgattaa a 21 <210> 126 <211> 19 <212> DNA <213> Brassica rapa <400> 126 cgggggttgc agaaagata 19 <210> 127 <211> 22 <212> DNA <213> Brassica rapa <400> 127 tcaacattgc catctaaaaa gg 22 <210> 128 <211> 22 <212> DNA <213> Brassica rapa <400> 128 tagactcatc aggttggctt ga 22 <210> 129 <211> 20 <212> DNA <213> Brassica rapa <400> 129 ttttcaactc ccacatgcac 20 <210> 130 <211> 21 <212> DNA <213> Brassica rapa <400> 130 tgggtatgtg ccaaattgtt t 21 <210> 131 <211> 21 <212> DNA <213> Brassica rapa <400> 131 ttccggtcac ttctagcttc a 21 <210> 132 <211> 24 <212> DNA <213> Brassica rapa <400> 132 ttttggtggt tagtatgtcg ctat 24 <210> 133 <211> 18 <212> DNA <213> Brassica rapa <400> 133 tatcaggaaa aggccacg 18 <210> 134 <211> 18 <212> DNA <213> Brassica rapa <400> 134 tcatcagcct tctctgcc 18 <210> 135 <211> 22 <212> DNA <213> Brassica rapa <400> 135 agctgctaaa gaggttgttg ac 22 <210> 136 <211> 22 <212> DNA <213> Brassica rapa <400> 136 aacatcagcc tgtcaagaga at 22 <210> 137 <211> 22 <212> DNA <213> Brassica rapa <400> 137 atgagaccgt gatgagaaat ga 22 <210> 138 <211> 22 <212> DNA <213> Brassica rapa <400> 138 gtgccacatc aacaagtagc at 22 <210> 139 <211> 24 <212> DNA <213> Brassica rapa <400> 139 tcgaaccgaa gtaaataacg gact 24 <210> 140 <211> 20 <212> DNA <213> Brassica rapa <400> 140 tttcgcccac tgacgctatt 20 <210> 141 <211> 26 <212> DNA <213> Brassica rapa <400> 141 tcgatgatta gtttagttat ttcacg 26 <210> 142 <211> 22 <212> DNA <213> Brassica rapa <400> 142 cctcaaacca aggaagattt ca 22 <210> 143 <211> 23 <212> DNA <213> Brassica rapa <400> 143 ttgtttcttg aacatctcgt cca 23 <210> 144 <211> 26 <212> DNA <213> Brassica rapa <400> 144 tcaagtctac ctcttgatga cgaaaa 26 <210> 145 <211> 20 <212> DNA <213> Brassica rapa <400> 145 acttcaaaac ccgaaaagcc 20 <210> 146 <211> 20 <212> DNA <213> Brassica rapa <400> 146 gatctcagag gggagggaga 20 <210> 147 <211> 20 <212> DNA <213> Brassica rapa <400> 147 tcgctctcac accccttctc 20 <210> 148 <211> 20 <212> DNA <213> Brassica rapa <400> 148 gtggcaaatt cggacagacc 20 <210> 149 <211> 22 <212> DNA <213> Brassica rapa <400> 149 ggtcaggtgc tactcagact cc 22 <210> 150 <211> 21 <212> DNA <213> Brassica rapa <400> 150 ttgaagagga tccaccaaaa g 21 <210> 151 <211> 20 <212> DNA <213> Brassica rapa <400> 151 gggtttgatg gttattcgag 20 <210> 152 <211> 20 <212> DNA <213> Brassica rapa <400> 152 ttcacctatg cgatgctttt 20 <210> 153 <211> 20 <212> DNA <213> Brassica rapa <400> 153 ttcaagcgta gcctagcaca 20 <210> 154 <211> 20 <212> DNA <213> Brassica rapa <400> 154 acgtgcaact gagtttgtgg 20 <210> 155 <211> 20 <212> DNA <213> Brassica rapa <400> 155 ctaaatgcgg cctttttaca 20 <210> 156 <211> 20 <212> DNA <213> Brassica rapa <400> 156 tagccttctt gttcagccac 20 <210> 157 <211> 20 <212> DNA <213> Brassica rapa <400> 157 acgccagtag gcctatcaaa 20 <210> 158 <211> 20 <212> DNA <213> Brassica rapa <400> 158 aagccaacaa tgccagaatc 20 <210> 159 <211> 22 <212> DNA <213> Brassica rapa <400> 159 cccaaaacat ccaaactcct ca 22 <210> 160 <211> 24 <212> DNA <213> Brassica rapa <400> 160 ccccaaagag atacagaaca aagc 24 <210> 161 <211> 20 <212> DNA <213> Brassica rapa <400> 161 tggaacggga aaagacataa 20 <210> 162 <211> 20 <212> DNA <213> Brassica rapa <400> 162 tgcaaacatc atcattccat 20 <210> 163 <211> 20 <212> DNA <213> Brassica rapa <400> 163 aaaaacccca tgttttctca 20 <210> 164 <211> 20 <212> DNA <213> Brassica rapa <400> 164 gaagttgaag ggaaagaggg 20 <210> 165 <211> 18 <212> DNA <213> Brassica rapa <400> 165 atggatcatc ccgcttct 18 <210> 166 <211> 18 <212> DNA <213> Brassica rapa <400> 166 cagggaggga tcaagagg 18 <210> 167 <211> 20 <212> DNA <213> Brassica rapa <400> 167 gaaagatgag agcgctgtgt 20 <210> 168 <211> 20 <212> DNA <213> Brassica rapa <400> 168 atgacaacga ggagcgtaag 20 <210> 169 <211> 22 <212> DNA <213> Brassica rapa <400> 169 caacaaacgc atttctctta ca 22 <210> 170 <211> 20 <212> DNA <213> Brassica rapa <400> 170 ctcctcgaga ccgtagacct 20 <210> 171 <211> 20 <212> DNA <213> Brassica rapa <400> 171 gtgagtgcga cacgtatgaa 20 <210> 172 <211> 20 <212> DNA <213> Brassica rapa <400> 172 tctttgtgct tctcctggtc 20 <210> 173 <211> 20 <212> DNA <213> Brassica rapa <400> 173 aaaatctcgc ttctgcgctt 20 <210> 174 <211> 20 <212> DNA <213> Brassica rapa <400> 174 tccctcaccc tgtgcaatag 20 <210> 175 <211> 20 <212> DNA <213> Brassica rapa <400> 175 gccacgtcaa tcctacaaag 20 <210> 176 <211> 22 <212> DNA <213> Brassica rapa <400> 176 ggggatactt agatggtcag aa 22 <210> 177 <211> 20 <212> DNA <213> Brassica rapa <400> 177 ctgaaccgaa ttggctaaaa 20 <210> 178 <211> 20 <212> DNA <213> Brassica rapa <400> 178 taaacagggg aactgagctg 20 <210> 179 <211> 20 <212> DNA <213> Brassica rapa <400> 179 gaggccaatt cttcaacaga 20 <210> 180 <211> 20 <212> DNA <213> Brassica rapa <400> 180 tgcttcattc cggtattcat 20 <210> 181 <211> 22 <212> DNA <213> Brassica rapa <400> 181 tcatagcata cagtgcattt tt 22 <210> 182 <211> 21 <212> DNA <213> Brassica rapa <400> 182 tggtttcaag ttagaccgat g 21 <210> 183 <211> 20 <212> DNA <213> Brassica rapa <400> 183 gttgctttta tgctgccgac 20 <210> 184 <211> 20 <212> DNA <213> Brassica rapa <400> 184 ggatttgtca aacgcgagac 20 <210> 185 <211> 20 <212> DNA <213> Brassica rapa <400> 185 tgcaaatcca agaaacaaaa 20 <210> 186 <211> 20 <212> DNA <213> Brassica rapa <400> 186 aagaggtgca agttccactg 20 <210> 187 <211> 20 <212> DNA <213> Brassica rapa <400> 187 attccggttt atctttcggc 20 <210> 188 <211> 20 <212> DNA <213> Brassica rapa <400> 188 ttgcgaaagt tcaaatgcct 20 <210> 189 <211> 22 <212> DNA <213> Brassica rapa <400> 189 gatgaaagta actggttgca ca 22 <210> 190 <211> 21 <212> DNA <213> Brassica rapa <400> 190 cggaaatcag gtcaatgtat g 21 <210> 191 <211> 22 <212> DNA <213> Brassica rapa <400> 191 tgaacctgtt tcatccaaga ga 22 <210> 192 <211> 22 <212> DNA <213> Brassica rapa <400> 192 tctggcatga tgtagttgct tt 22 <210> 193 <211> 20 <212> DNA <213> Brassica rapa <400> 193 cggagataac cggaatggaa 20 <210> 194 <211> 21 <212> DNA <213> Brassica rapa <400> 194 ggatgctctg agacacccaa a 21 <210> 195 <211> 24 <212> DNA <213> Brassica rapa <400> 195 tgaaaatcaa cacgaacaca caga 24 <210> 196 <211> 20 <212> DNA <213> Brassica rapa <400> 196 ctcgtggggg aatgagtgag 20 <210> 197 <211> 24 <212> DNA <213> Brassica rapa <400> 197 cgacggatct gaagagagta gagg 24 <210> 198 <211> 22 <212> DNA <213> Brassica rapa <400> 198 cgaaacagag gaaacatcac cc 22 <210> 199 <211> 22 <212> DNA <213> Brassica rapa <400> 199 gaagaagacg aagttgagct gt 22 <210> 200 <211> 22 <212> DNA <213> Brassica rapa <400> 200 gcccatattg atacttaacg ga 22 <210> 201 <211> 21 <212> DNA <213> Brassica rapa <400> 201 aagcttgagc ttccagcctt c 21 <210> 202 <211> 20 <212> DNA <213> Brassica rapa <400> 202 atcagtgccg gccttgaata 20 <210> 203 <211> 24 <212> DNA <213> Brassica rapa <400> 203 ttgaaataca tgatacccca acca 24 <210> 204 <211> 20 <212> DNA <213> Brassica rapa <400> 204 ccgttttcag ggcacagttt 20 <210> 205 <211> 18 <212> DNA <213> Brassica rapa <400> 205 agtggtcccc acgagaat 18 <210> 206 <211> 18 <212> DNA <213> Brassica rapa <400> 206 tcgagaaggt cggtgaga 18 <210> 207 <211> 20 <212> DNA <213> Brassica rapa <400> 207 tcatcagctg cacgaaagta 20 <210> 208 <211> 20 <212> DNA <213> Brassica rapa <400> 208 atgaagaatg gtgcatggat 20 <210> 209 <211> 22 <212> DNA <213> Brassica rapa <400> 209 tcggttaaat cgagtaacga tg 22 <210> 210 <211> 23 <212> DNA <213> Brassica rapa <400> 210 tttcaggacc tagacgttac caa 23 <210> 211 <211> 22 <212> DNA <213> Brassica rapa <400> 211 tcattggtga agaacccaca aa 22 <210> 212 <211> 24 <212> DNA <213> Brassica rapa <400> 212 gcgaccataa aaagagagtg agaa 24 <210> 213 <211> 21 <212> DNA <213> Brassica rapa <400> 213 gtccatctcc tacctgctcc a 21 <210> 214 <211> 22 <212> DNA <213> Brassica rapa <400> 214 gttttgagcc gaataatggt tg 22 <210> 215 <211> 22 <212> DNA <213> Brassica rapa <400> 215 cgatttggac aatgactagt gg 22 <210> 216 <211> 20 <212> DNA <213> Brassica rapa <400> 216 aacgccatgg aaacagaaac 20 <210> 217 <211> 20 <212> DNA <213> Brassica rapa <400> 217 tccaaatcca actcttccca 20 <210> 218 <211> 20 <212> DNA <213> Brassica rapa <400> 218 ccccatttgg aagatgctaa 20 <210> 219 <211> 20 <212> DNA <213> Brassica rapa <400> 219 gcagcaaaac ctctcccttt 20 <210> 220 <211> 20 <212> DNA <213> Brassica rapa <400> 220 ccttcagagg tgcgtttgac 20 <210> 221 <211> 20 <212> DNA <213> Brassica rapa <400> 221 aaacgtggaa gcacattttt 20 <210> 222 <211> 20 <212> DNA <213> Brassica rapa <400> 222 agtcacgacg tgtttcatgt 20 <210> 223 <211> 20 <212> DNA <213> Brassica rapa <400> 223 aatttttaga aggaggcgga 20 <210> 224 <211> 20 <212> DNA <213> Brassica rapa <400> 224 caccatcaca acaacaagga 20 <210> 225 <211> 20 <212> DNA <213> Brassica rapa <400> 225 aaatctccca acatggttca 20 <210> 226 <211> 22 <212> DNA <213> Brassica rapa <400> 226 agatgccatt actcctaact tg 22 <210> 227 <211> 20 <212> DNA <213> Brassica rapa <400> 227 cagacggttg catgaagata 20 <210> 228 <211> 22 <212> DNA <213> Brassica rapa <400> 228 agatgtgggt aattgcagtt tt 22 <210> 229 <211> 25 <212> DNA <213> Brassica rapa <400> 229 cccaaatacg aaaacaaagt ttgac 25 <210> 230 <211> 20 <212> DNA <213> Brassica rapa <400> 230 aggatctcat ccgctttcca 20 <210> 231 <211> 25 <212> DNA <213> Brassica rapa <400> 231 aaaaagaaat ggatattgtg tgaaa 25 <210> 232 <211> 20 <212> DNA <213> Brassica rapa <400> 232 cctggatcaa gaccacgaag 20 <210> 233 <211> 22 <212> DNA <213> Brassica rapa <400> 233 tgcttttaac caaactcaaa cg 22 <210> 234 <211> 20 <212> DNA <213> Brassica rapa <400> 234 tgcagagagg caagtttcaa 20 <210> 235 <211> 24 <212> DNA <213> Brassica rapa <400> 235 tgcatgatgt tcatgtcttg taaa 24 <210> 236 <211> 25 <212> DNA <213> Brassica rapa <400> 236 tccttctgta aaccggttgt aattt 25 <210> 237 <211> 20 <212> DNA <213> Brassica rapa <400> 237 tcgtttgacc aaccgtacaa 20 <210> 238 <211> 20 <212> DNA <213> Brassica rapa <400> 238 cttgccagcg ttgatacaga 20 <210> 239 <211> 25 <212> DNA <213> Brassica rapa <400> 239 tgttcatgtt catcagccct ctcaa 25 <210> 240 <211> 25 <212> DNA <213> Brassica rapa <400> 240 tcttttctgc cctcacctac aatca 25 <210> 241 <211> 20 <212> DNA <213> Brassica rapa <400> 241 tcgggattgc tatcaacagt 20 <210> 242 <211> 20 <212> DNA <213> Brassica rapa <400> 242 agctctcaag cgaaattcca 20 <210> 243 <211> 20 <212> DNA <213> Brassica rapa <400> 243 ctcctggaaa gaacaccaca 20 <210> 244 <211> 20 <212> DNA <213> Brassica rapa <400> 244 aaagcacttg agccagcttc 20 <210> 245 <211> 18 <212> DNA <213> Brassica rapa <400> 245 gggctcttcc aacaggtc 18 <210> 246 <211> 18 <212> DNA <213> Brassica rapa <400> 246 tgtgagatcg ccgagagt 18 <210> 247 <211> 27 <212> DNA <213> Brassica rapa <400> 247 aaccttcatt tcatatacat acacaca 27 <210> 248 <211> 24 <212> DNA <213> Brassica rapa <400> 248 ttcaatcatt tttattggtc atca 24 <210> 249 <211> 19 <212> DNA <213> Brassica rapa <400> 249 gcgcaaaacg catatctta 19 <210> 250 <211> 23 <212> DNA <213> Brassica rapa <400> 250 tcttttgatc ctttgtttct tca 23 <210> 251 <211> 23 <212> DNA <213> Brassica rapa <400> 251 ttgaaatgcg acttttatat gac 23 <210> 252 <211> 20 <212> DNA <213> Brassica rapa <400> 252 ccggttcaaa atatgacaca 20 <210> 253 <211> 17 <212> DNA <213> Brassica rapa <400> 253 cgcagccttt tgcttct 17 <210> 254 <211> 18 <212> DNA <213> Brassica rapa <400> 254 ttgctctcct gcagcttg 18 <210> 255 <211> 18 <212> DNA <213> Brassica rapa <400> 255 cgataccaac gcaccact 18 <210> 256 <211> 19 <212> DNA <213> Brassica rapa <400> 256 caacctccac atttttcca 19 <210> 257 <211> 24 <212> DNA <213> Brassica rapa <400> 257 tgctttaatc aatccaaatt cgtt 24 <210> 258 <211> 26 <212> DNA <213> Brassica rapa <400> 258 ttgtaccgag ttaagctgaa aaattc 26 <210> 259 <211> 22 <212> DNA <213> Brassica rapa <400> 259 gagatcacga aagctgtaga gg 22 <210> 260 <211> 22 <212> DNA <213> Brassica rapa <400> 260 cgaacttgaa agccagtaat tc 22 <210> 261 <211> 20 <212> DNA <213> Brassica rapa <400> 261 gaggaattaa cggcgtcttg 20 <210> 262 <211> 20 <212> DNA <213> Brassica rapa <400> 262 cagtcgccac tacctggttt 20 <210> 263 <211> 22 <212> DNA <213> Brassica rapa <400> 263 ctgttgaaaa cacagcacaa gt 22 <210> 264 <211> 22 <212> DNA <213> Brassica rapa <400> 264 ggtcgatctg ccatgtatta ct 22 <210> 265 <211> 27 <212> DNA <213> Brassica rapa <400> 265 tgtgttaaac tatctccgaa ctagaaa 27 <210> 266 <211> 27 <212> DNA <213> Brassica rapa <400> 266 tttctctagg atattagaaa aggaaaa 27 <210> 267 <211> 20 <212> DNA <213> Brassica rapa <400> 267 acttggagct cttggtatcg 20 <210> 268 <211> 19 <212> DNA <213> Brassica rapa <400> 268 aaacccttaa acctcgtcg 19 <210> 269 <211> 23 <212> DNA <213> Brassica rapa <400> 269 tcctaattga agccttggtc ata 23 <210> 270 <211> 23 <212> DNA <213> Brassica rapa <400> 270 cacttggcag aagaagagaa aaa 23 <210> 271 <211> 20 <212> DNA <213> Brassica rapa <400> 271 gattggcctg agacagaaga 20 <210> 272 <211> 20 <212> DNA <213> Brassica rapa <400> 272 ccagagcgcg aactctacta 20 <210> 273 <211> 20 <212> DNA <213> Brassica rapa <400> 273 catgtcaaac caacgaaaca 20 <210> 274 <211> 20 <212> DNA <213> Brassica rapa <400> 274 gatgaacttc gtgatttggg 20 <210> 275 <211> 20 <212> DNA <213> Brassica rapa <400> 275 gatacacaag gttcaacgcc 20 <210> 276 <211> 20 <212> DNA <213> Brassica rapa <400> 276 ttccttagct tgtgactggc 20 <210> 277 <211> 20 <212> DNA <213> Brassica rapa <400> 277 atcagaaccg aatccgacca 20 <210> 278 <211> 23 <212> DNA <213> Brassica rapa <400> 278 caatggttgc aatgttattt gga 23 <210> 279 <211> 27 <212> DNA <213> Brassica rapa <400> 279 cgagtttttg tgtgtacgta tagtaat 27 <210> 280 <211> 20 <212> DNA <213> Brassica rapa <400> 280 ccaaagtgcg taaaggaagg 20 <210> 281 <211> 26 <212> DNA <213> Brassica rapa <400> 281 ttatcaacat attttcaatc attcca 26 <210> 282 <211> 26 <212> DNA <213> Brassica rapa <400> 282 gctttggact atgcttctaa agtacg 26 <210> 283 <211> 23 <212> DNA <213> Brassica rapa <400> 283 tgtaaagttg tgcaaggatt gtg 23 <210> 284 <211> 24 <212> DNA <213> Brassica rapa <400> 284 tgggtttgtg aaaatatggt gaaa 24 <210> 285 <211> 20 <212> DNA <213> Brassica rapa <400> 285 ggagacgttg tcttcacctg 20 <210> 286 <211> 20 <212> DNA <213> Brassica rapa <400> 286 gttggggaga accagcttat 20 <210> 287 <211> 21 <212> DNA <213> Brassica rapa <400> 287 tttgatcgtc tttttcgttt g 21 <210> 288 <211> 20 <212> DNA <213> Brassica rapa <400> 288 gagcgggtag gttactggtt 20 <210> 289 <211> 21 <212> DNA <213> Brassica rapa <400> 289 gatgtgaggg taagtttgtg g 21 <210> 290 <211> 22 <212> DNA <213> Brassica rapa <400> 290 caatgaatgt gatgttatcc ga 22 <210> 291 <211> 20 <212> DNA <213> Brassica rapa <400> 291 gcatgatttt tgacaggctt 20 <210> 292 <211> 20 <212> DNA <213> Brassica rapa <400> 292 tgtcacgcgt cattcataga 20 <210> 293 <211> 20 <212> DNA <213> Brassica rapa <400> 293 ttcattctcc ttttgcgttc 20 <210> 294 <211> 20 <212> DNA <213> Brassica rapa <400> 294 cgcgtttcac ttcttcttgt 20 <210> 295 <211> 20 <212> DNA <213> Brassica rapa <400> 295 accattgaca gaagctggag 20 <210> 296 <211> 20 <212> DNA <213> Brassica rapa <400> 296 ttgcatcctt ttgtcacgta 20 <210> 297 <211> 23 <212> DNA <213> Brassica rapa <400> 297 cgagattttc gtataacgat tca 23 <210> 298 <211> 22 <212> DNA <213> Brassica rapa <400> 298 aaatcagact cactcgacca tt 22 <210> 299 <211> 22 <212> DNA <213> Brassica rapa <400> 299 aaagggagtt gaagtaagag gc 22 <210> 300 <211> 22 <212> DNA <213> Brassica rapa <400> 300 ctggacaagt gttctagtgc aa 22 <210> 301 <211> 26 <212> DNA <213> Brassica rapa <400> 301 gagagaagaa gaagaaggaa gcagaa 26 <210> 302 <211> 19 <212> DNA <213> Brassica rapa <400> 302 gatctcgcgt gtggcaact 19 <210> 303 <211> 22 <212> DNA <213> Brassica rapa <400> 303 gctaagaaag gagacaacat gg 22 <210> 304 <211> 22 <212> DNA <213> Brassica rapa <400> 304 gaaccttctg gttgaatgtg tt 22 <210> 305 <211> 27 <212> DNA <213> Brassica rapa <400> 305 gaaacaaatt atttaaaaat cagacca 27 <210> 306 <211> 23 <212> DNA <213> Brassica rapa <400> 306 tggaacaatc cgtaaaacta tgc 23 <210> 307 <211> 20 <212> DNA <213> Brassica rapa <400> 307 ataagggcat cgcctcaaca 20 <210> 308 <211> 20 <212> DNA <213> Brassica rapa <400> 308 tgcacgcatc cacataaaca 20 <210> 309 <211> 21 <212> DNA <213> Brassica rapa <400> 309 ggaccgactt tagcaagtcc a 21 <210> 310 <211> 27 <212> DNA <213> Brassica rapa <400> 310 gggtagctta gaagatcatc tctttgg 27 <210> 311 <211> 20 <212> DNA <213> Brassica rapa <400> 311 aggccaccac gtgattctaa 20 <210> 312 <211> 20 <212> DNA <213> Brassica rapa <400> 312 cacatatgtc cccttttcgc 20 <210> 313 <211> 20 <212> DNA <213> Brassica rapa <400> 313 tcttgcgaat gttccggttt 20 <210> 314 <211> 26 <212> DNA <213> Brassica rapa <400> 314 ccaaaattaa gagaataaat gattgg 26 <210> 315 <211> 20 <212> DNA <213> Brassica rapa <400> 315 tgatgtcgaa gaggctggat 20 <210> 316 <211> 20 <212> DNA <213> Brassica rapa <400> 316 gactcaaacc ctgtggacga 20 <210> 317 <211> 22 <212> DNA <213> Brassica rapa <400> 317 gagaaatcgc cacatatagc tc 22 <210> 318 <211> 22 <212> DNA <213> Brassica rapa <400> 318 gatttcaaaa tgcaagaagg tc 22 <210> 319 <211> 20 <212> DNA <213> Brassica rapa <400> 319 ctgcgcgtca gtcactacaa 20 <210> 320 <211> 20 <212> DNA <213> Brassica rapa <400> 320 agtctaccca aatgcggaca 20 <210> 321 <211> 20 <212> DNA <213> Brassica rapa <400> 321 ccaaggatga tggaggcata 20 <210> 322 <211> 20 <212> DNA <213> Brassica rapa <400> 322 tgactttcca tttccgtcgt 20 <210> 323 <211> 19 <212> DNA <213> Brassica rapa <400> 323 cgaacgaaca gaaacaagc 19 <210> 324 <211> 18 <212> DNA <213> Brassica rapa <400> 324 aacgaggcaa gggctaaa 18 <210> 325 <211> 19 <212> DNA <213> Brassica rapa <400> 325 ctggaccctg ccacacaag 19 <210> 326 <211> 22 <212> DNA <213> Brassica rapa <400> 326 gagtaggagt ttccgacgag ga 22 <210> 327 <211> 21 <212> DNA <213> Brassica rapa <400> 327 aactggaaac tccttggcac t 21 <210> 328 <211> 20 <212> DNA <213> Brassica rapa <400> 328 gtccaacttg atgcatttcg 20 <210> 329 <211> 22 <212> DNA <213> Brassica rapa <400> 329 aattcgtctt gcgtcggtaa aa 22 <210> 330 <211> 22 <212> DNA <213> Brassica rapa <400> 330 gcctagagat aaacgtggac cg 22 <210> 331 <211> 22 <212> DNA <213> Brassica rapa <400> 331 cagagcaatc gaagagaaga at 22 <210> 332 <211> 22 <212> DNA <213> Brassica rapa <400> 332 ttaggtgggt ctcaaatcaa at 22 <210> 333 <211> 20 <212> DNA <213> Brassica rapa <400> 333 cctatgtctc agccgtccat 20 <210> 334 <211> 20 <212> DNA <213> Brassica rapa <400> 334 taccaccgcc attaccattt 20 <210> 335 <211> 22 <212> DNA <213> Brassica rapa <400> 335 aaagattggg ttgtgagatt tg 22 <210> 336 <211> 22 <212> DNA <213> Brassica rapa <400> 336 atgttcgatt gcttctgaaa ct 22 <210> 337 <211> 19 <212> DNA <213> Brassica rapa <400> 337 ggaacgagtt gaacaccaa 19 <210> 338 <211> 18 <212> DNA <213> Brassica rapa <400> 338 ccgtcaaacc aatctcca 18 <210> 339 <211> 20 <212> DNA <213> Brassica rapa <400> 339 gccatgtggc cttctaaaaa 20 <210> 340 <211> 20 <212> DNA <213> Brassica rapa <400> 340 cgattcctgg tcatttggat 20 <210> 341 <211> 22 <212> DNA <213> Brassica rapa <400> 341 tcggttagag atctggagaa ga 22 <210> 342 <211> 22 <212> DNA <213> Brassica rapa <400> 342 aaaaatcaaa gtggcagcta aa 22 <210> 343 <211> 20 <212> DNA <213> Brassica rapa <400> 343 acgtggcatt cattaaacgg 20 <210> 344 <211> 22 <212> DNA <213> Brassica rapa <400> 344 gaaagagaga tccttcagcc aa 22 <210> 345 <211> 25 <212> DNA <213> Brassica rapa <400> 345 ttggatcaat caaactaaac cctga 25 <210> 346 <211> 20 <212> DNA <213> Brassica rapa <400> 346 ccaaaatgcc aacaaaagca 20 <210> 347 <211> 21 <212> DNA <213> Brassica rapa <400> 347 gggatattga agacccgcaa a 21 <210> 348 <211> 20 <212> DNA <213> Brassica rapa <400> 348 tctcccggtg gcttaaagaa 20 <210> 349 <211> 22 <212> DNA <213> Brassica rapa <400> 349 ttaatcatct gatgtcctcg aa 22 <210> 350 <211> 22 <212> DNA <213> Brassica rapa <400> 350 aacaacatca gttatcatgc ca 22 <210> 351 <211> 20 <212> DNA <213> Brassica rapa <400> 351 agttggattc tcgttgggaa 20 <210> 352 <211> 21 <212> DNA <213> Brassica rapa <400> 352 tgagaggtcc accttttgtc a 21 <210> 353 <211> 20 <212> DNA <213> Brassica rapa <400> 353 cgccctaaag tccggtagat 20 <210> 354 <211> 20 <212> DNA <213> Brassica rapa <400> 354 caaagcaagc gtaagaggga 20 <210> 355 <211> 20 <212> DNA <213> Brassica rapa <400> 355 tgggtaaaca aatggaggtg 20 <210> 356 <211> 20 <212> DNA <213> Brassica rapa <400> 356 ctcatctttc ccctccaaac 20 <210> 357 <211> 22 <212> DNA <213> Brassica rapa <400> 357 acgtaataat gttggttctc cg 22 <210> 358 <211> 22 <212> DNA <213> Brassica rapa <400> 358 tgattctcct tctccactgt ct 22 <210> 359 <211> 21 <212> DNA <213> Brassica rapa <400> 359 caagcaatca gagctttttg a 21 <210> 360 <211> 20 <212> DNA <213> Brassica rapa <400> 360 cagtctgtta tggtgtgcca 20 <210> 361 <211> 22 <212> DNA <213> Brassica rapa <400> 361 caaactctcc ttccacatgt ca 22 <210> 362 <211> 22 <212> DNA <213> Brassica rapa <400> 362 ttcttttaag taacctagtt ag 22 <210> 363 <211> 20 <212> DNA <213> Brassica rapa <400> 363 agaaagggaa atcaggaccg 20 <210> 364 <211> 20 <212> DNA <213> Brassica rapa <400> 364 caggctttac attccggttg 20 <210> 365 <211> 20 <212> DNA <213> Brassica rapa <400> 365 ccaacaacaa ccatggtgaa 20 <210> 366 <211> 20 <212> DNA <213> Brassica rapa <400> 366 caaactggag caaagcatca 20 <210> 367 <211> 20 <212> DNA <213> Brassica rapa <400> 367 ccactaccaa gccattgtga 20 <210> 368 <211> 20 <212> DNA <213> Brassica rapa <400> 368 gagaccacgc aagatcaggt 20 <210> 369 <211> 26 <212> DNA <213> Brassica rapa <400> 369 aattagcgcg ataacataaa taaaaa 26 <210> 370 <211> 27 <212> DNA <213> Brassica rapa <400> 370 aaattgctta actattaaaa ctgcaaa 27 <210> 371 <211> 20 <212> DNA <213> Brassica rapa <400> 371 cgagatgagc ttcgaacaat 20 <210> 372 <211> 20 <212> DNA <213> Brassica rapa <400> 372 aaaaagcgag ttgggaaaag 20 <210> 373 <211> 20 <212> DNA <213> Brassica rapa <400> 373 agccgttgca gatcatctta 20 <210> 374 <211> 25 <212> DNA <213> Brassica rapa <400> 374 tcgattaacc taagatgaag acagg 25 <210> 375 <211> 20 <212> DNA <213> Brassica rapa <400> 375 tgtcgccatt ttactgttcg 20 <210> 376 <211> 20 <212> DNA <213> Brassica rapa <400> 376 ggaaagaagg ttgtggcaaa 20 <210> 377 <211> 20 <212> DNA <213> Brassica rapa <400> 377 gttttgtggc cttcgagttt 20 <210> 378 <211> 26 <212> DNA <213> Brassica rapa <400> 378 tttggagaca tacaaatcgt taagag 26 <210> 379 <211> 20 <212> DNA <213> Brassica rapa <400> 379 aaactaggta aggaacccgc 20 <210> 380 <211> 20 <212> DNA <213> Brassica rapa <400> 380 aaacgaacaa ttgcaagtcc 20 <210> 381 <211> 20 <212> DNA <213> Brassica rapa <400> 381 gttgctgggc ttgcagttat 20 <210> 382 <211> 20 <212> DNA <213> Brassica rapa <400> 382 gagcgtacca gcaacctctc 20 <210> 383 <211> 22 <212> DNA <213> Brassica rapa <400> 383 ccaagaaatt ggtcatgatt tt 22 <210> 384 <211> 22 <212> DNA <213> Brassica rapa <400> 384 aatgcgtttt gtgtagcact tt 22 <210> 385 <211> 23 <212> DNA <213> Brassica rapa <400> 385 tttttggagc acacatctaa caa 23 <210> 386 <211> 25 <212> DNA <213> Brassica rapa <400> 386 tgggaaatat tcctaatctc agtca 25 <210> 387 <211> 22 <212> DNA <213> Brassica rapa <400> 387 gaggaagtac ggcaagaaac ca 22 <210> 388 <211> 22 <212> DNA <213> Brassica rapa <400> 388 aggacacatg tccacatgaa aa 22 <210> 389 <211> 20 <212> DNA <213> Brassica rapa <400> 389 tttcggtttg gtttggtttc 20 <210> 390 <211> 27 <212> DNA <213> Brassica rapa <400> 390 caaactagtt ggattcacaa ttataca 27 <210> 391 <211> 18 <212> DNA <213> Brassica rapa <400> 391 tggagaaggg gtctccat 18 <210> 392 <211> 21 <212> DNA <213> Brassica rapa <400> 392 ttgttcctaa caaacggaaa a 21 <210> 393 <211> 20 <212> DNA <213> Brassica rapa <400> 393 gtcgtcaggc aaaggtggtt 20 <210> 394 <211> 20 <212> DNA <213> Brassica rapa <400> 394 ggcggcagaa agaaagaaag 20 <210> 395 <211> 24 <212> DNA <213> Brassica rapa <400> 395 acacctactt gtttccatcc aaat 24 <210> 396 <211> 20 <212> DNA <213> Brassica rapa <400> 396 cgggtatttg cgttgtttcc 20 <210> 397 <211> 22 <212> DNA <213> Brassica rapa <400> 397 agtcggagga aacgcgaaat ta 22 <210> 398 <211> 27 <212> DNA <213> Brassica rapa <400> 398 cgaaataaag acagacagag acatcca 27 <210> 399 <211> 20 <212> DNA <213> Brassica rapa <400> 399 tcgcgaggaa tagaacgaat 20 <210> 400 <211> 21 <212> DNA <213> Brassica rapa <400> 400 ggaaccttct cgatggtttt t 21 <210> 401 <211> 25 <212> DNA <213> Brassica rapa <400> 401 ttcatccact ttgttcaata caaga 25 <210> 402 <211> 23 <212> DNA <213> Brassica rapa <400> 402 ccgtcaaaaa cgaagtaaat cac 23 <210> 403 <211> 20 <212> DNA <213> Brassica rapa <400> 403 tgtgtcgctc gtctacgtct 20 <210> 404 <211> 20 <212> DNA <213> Brassica rapa <400> 404 accatcgact tcgtggaaac 20 <210> 405 <211> 20 <212> DNA <213> Brassica rapa <400> 405 gccgactcct agtgaggaaa 20 <210> 406 <211> 20 <212> DNA <213> Brassica rapa <400> 406 tgtgttttgg gctcaaaggt 20 <210> 407 <211> 22 <212> DNA <213> Brassica rapa <400> 407 ctgctccttc acgttttcat ca 22 <210> 408 <211> 22 <212> DNA <213> Brassica rapa <400> 408 acggacagca acaacaacaa ga 22 <210> 409 <211> 20 <212> DNA <213> Brassica rapa <400> 409 ggagcttgtt caatcctcca 20 <210> 410 <211> 20 <212> DNA <213> Brassica rapa <400> 410 tgtacctgcg gcttctttct 20 <210> 411 <211> 23 <212> DNA <213> Brassica rapa <400> 411 gcctgcacta aaatagctgc aaa 23 <210> 412 <211> 20 <212> DNA <213> Brassica rapa <400> 412 gaatggaagg cgtcgatcat 20 <210> 413 <211> 20 <212> DNA <213> Brassica rapa <400> 413 ttttggagat tagtgtgccc 20 <210> 414 <211> 20 <212> DNA <213> Brassica rapa <400> 414 agtaaggcag agcagtgtgc 20 <210> 415 <211> 18 <212> DNA <213> Brassica rapa <400> 415 tccacgcaaa accaaaac 18 <210> 416 <211> 18 <212> DNA <213> Brassica rapa <400> 416 tcatcccctc cgttcttt 18 <210> 417 <211> 20 <212> DNA <213> Brassica rapa <400> 417 gggccaagtt acatggaaaa 20 <210> 418 <211> 20 <212> DNA <213> Brassica rapa <400> 418 gaaggaggat gagagccgtt 20 <210> 419 <211> 20 <212> DNA <213> Brassica rapa <400> 419 caatcgtttt acatgagccc 20 <210> 420 <211> 20 <212> DNA <213> Brassica rapa <400> 420 ccaagtgctt agtggttggt 20 <210> 421 <211> 26 <212> DNA <213> Brassica rapa <400> 421 tctttcttga aaagatttgg ttccat 26 <210> 422 <211> 21 <212> DNA <213> Brassica rapa <400> 422 tgggcattta gctacgacct t 21 <210> 423 <211> 20 <212> DNA <213> Brassica rapa <400> 423 tggatgttgt ttggttgatg 20 <210> 424 <211> 20 <212> DNA <213> Brassica rapa <400> 424 tgtccttttc tcaattccca 20 <210> 425 <211> 22 <212> DNA <213> Brassica rapa <400> 425 tgccaatctc agatcttttt ca 22 <210> 426 <211> 20 <212> DNA <213> Brassica rapa <400> 426 tctctcctat agccgccatc 20 <210> 427 <211> 22 <212> DNA <213> Brassica rapa <400> 427 gccacaacaa agtattgtct ca 22 <210> 428 <211> 23 <212> DNA <213> Brassica rapa <400> 428 aacatctatc ttactggaac gga 23 <210> 429 <211> 20 <212> DNA <213> Brassica rapa <400> 429 cggtgaccag taagctgttt 20 <210> 430 <211> 20 <212> DNA <213> Brassica rapa <400> 430 tgtctgtttc cacgtcaatg 20 <210> 431 <211> 21 <212> DNA <213> Brassica rapa <400> 431 ttcgattgtg gttcttattg g 21 <210> 432 <211> 23 <212> DNA <213> Brassica rapa <400> 432 ccatatttat cttagttctc gcc 23 <210> 433 <211> 21 <212> DNA <213> Brassica rapa <400> 433 aatgagtaat gtcccacacg a 21 <210> 434 <211> 21 <212> DNA <213> Brassica rapa <400> 434 tgaaattgcg gattctttag c 21 <210> 435 <211> 23 <212> DNA <213> Brassica rapa <400> 435 tcatctaaaa gatcaataag cga 23 <210> 436 <211> 20 <212> DNA <213> Brassica rapa <400> 436 agtcggatca tcctcggtat 20 <210> 437 <211> 22 <212> DNA <213> Brassica rapa <400> 437 ctctcgtctc ggaggatcta aa 22 <210> 438 <211> 22 <212> DNA <213> Brassica rapa <400> 438 gtgagagtgg ttgctgagtg ag 22 <210> 439 <211> 20 <212> DNA <213> Brassica rapa <400> 439 tgggtaattt gggaatttga 20 <210> 440 <211> 20 <212> DNA <213> Brassica rapa <400> 440 attgtacgtt gacgatggct 20 <210> 441 <211> 22 <212> DNA <213> Brassica rapa <400> 441 acctctgact gaaaatgaag tt 22 <210> 442 <211> 21 <212> DNA <213> Brassica rapa <400> 442 tgcatgtact ccctatgttc a 21 <210> 443 <211> 23 <212> DNA <213> Brassica rapa <400> 443 aacacttgat tttagaaact gga 23 <210> 444 <211> 22 <212> DNA <213> Brassica rapa <400> 444 ttgtagaact tgtgtgattg gg 22 <210> 445 <211> 20 <212> DNA <213> Brassica rapa <400> 445 tgccaacaaa tcaaggatgc 20 <210> 446 <211> 24 <212> DNA <213> Brassica rapa <400> 446 ccgaagttca cttgttattc caac 24 <210> 447 <211> 20 <212> DNA <213> Brassica rapa <400> 447 agaaagcatg cgtgagaaac 20 <210> 448 <211> 20 <212> DNA <213> Brassica rapa <400> 448 aagttgtccg gagttcacaa 20 <210> 449 <211> 20 <212> DNA <213> Brassica rapa <400> 449 tccaagatgg tgtgtgtgag 20 <210> 450 <211> 20 <212> DNA <213> Brassica rapa <400> 450 cgcaactatt gacgctcttt 20 <210> 451 <211> 20 <212> DNA <213> Brassica rapa <400> 451 gtggaggcaa aatttggtaa 20 <210> 452 <211> 20 <212> DNA <213> Brassica rapa <400> 452 gcaattgggt gttcagtttc 20 <210> 453 <211> 20 <212> DNA <213> Brassica rapa <400> 453 tcttctattg gcaatctggt 20 <210> 454 <211> 20 <212> DNA <213> Brassica rapa <400> 454 ttgttagggc tcgttgagac 20 <210> 455 <211> 22 <212> DNA <213> Brassica rapa <400> 455 ttcatacgag catctgcata ac 22 <210> 456 <211> 22 <212> DNA <213> Brassica rapa <400> 456 ttaaaccact tgaaccgaaa ct 22 <210> 457 <211> 20 <212> DNA <213> Brassica rapa <400> 457 ctaaggccaa gaaagggaag 20 <210> 458 <211> 20 <212> DNA <213> Brassica rapa <400> 458 tgcttatgcc cagataaagc 20 <210> 459 <211> 23 <212> DNA <213> Brassica rapa <400> 459 ccggtatgaa tattccattg cta 23 <210> 460 <211> 22 <212> DNA <213> Brassica rapa <400> 460 tgaaatcttc ctcccaaaga aa 22 <210> 461 <211> 23 <212> DNA <213> Brassica rapa <400> 461 ggttgacgtc tcattgtgtt ctt 23 <210> 462 <211> 23 <212> DNA <213> Brassica rapa <400> 462 tagctttgct cacttttcac tcc 23 <210> 463 <211> 23 <212> DNA <213> Brassica rapa <400> 463 acaattgttc taggcgttca aaa 23 <210> 464 <211> 23 <212> DNA <213> Brassica rapa <400> 464 atcagtatcc tggttctggt tca 23 <210> 465 <211> 20 <212> DNA <213> Brassica rapa <400> 465 gtatccttgg tgggcctagt 20 <210> 466 <211> 20 <212> DNA <213> Brassica rapa <400> 466 tgaccatctc aatgactcca 20 <210> 467 <211> 23 <212> DNA <213> Brassica rapa <400> 467 acacaaaggc aaaagagtag ctg 23 <210> 468 <211> 23 <212> DNA <213> Brassica rapa <400> 468 gaacctaatg aaatgcacaa agc 23 <210> 469 <211> 20 <212> DNA <213> Brassica rapa <400> 469 tcaccgccat aatttgatcc 20 <210> 470 <211> 21 <212> DNA <213> Brassica rapa <400> 470 ccctctcaac aaggtatgca a 21 <210> 471 <211> 20 <212> DNA <213> Brassica rapa <400> 471 gggtatttcc ggtttaggaa 20 <210> 472 <211> 20 <212> DNA <213> Brassica rapa <400> 472 ccctcttcct ctcaacacct 20 <210> 473 <211> 20 <212> DNA <213> Brassica rapa <400> 473 ttcccaagct tgctggtact 20 <210> 474 <211> 20 <212> DNA <213> Brassica rapa <400> 474 gagatttccc tcgcttgatg 20 <210> 475 <211> 20 <212> DNA <213> Brassica rapa <400> 475 cacgtcgggt taaattcttg 20 <210> 476 <211> 20 <212> DNA <213> Brassica rapa <400> 476 tgtttagggg ctcctcattt 20 <210> 477 <211> 20 <212> DNA <213> Brassica rapa <400> 477 cgggcagact gtttctgtat 20 <210> 478 <211> 20 <212> DNA <213> Brassica rapa <400> 478 ttcctccttt gaaccaatca 20 <210> 479 <211> 20 <212> DNA <213> Brassica rapa <400> 479 acttctccgg ctggatactc 20 <210> 480 <211> 20 <212> DNA <213> Brassica rapa <400> 480 ccgtttaaac ttgcgtttgt 20 <210> 481 <211> 23 <212> DNA <213> Brassica rapa <400> 481 cagtgtgatg ttgaaagaga atg 23 <210> 482 <211> 22 <212> DNA <213> Brassica rapa <400> 482 tcaaatctag agaatcccct ct 22 <210> 483 <211> 20 <212> DNA <213> Brassica rapa <400> 483 ccgtgaccgc ttcttacgat 20 <210> 484 <211> 20 <212> DNA <213> Brassica rapa <400> 484 caggcctttt aaaccgcaac 20 <210> 485 <211> 23 <212> DNA <213> Brassica rapa <400> 485 tgcctttata agagttagac cga 23 <210> 486 <211> 20 <212> DNA <213> Brassica rapa <400> 486 aacttttcca atgtgtgctt 20 <210> 487 <211> 19 <212> DNA <213> Brassica rapa <400> 487 ttcacacaag gtttgtgcc 19 <210> 488 <211> 20 <212> DNA <213> Brassica rapa <400> 488 cgtaaaggca tcaaggaaaa 20 <210> 489 <211> 19 <212> DNA <213> Brassica rapa <400> 489 gaatttctga cttggcgct 19 <210> 490 <211> 23 <212> DNA <213> Brassica rapa <400> 490 aaaacaaggt tgaatactac gca 23 <210> 491 <211> 18 <212> DNA <213> Brassica rapa <400> 491 ggacagccga catagctg 18 <210> 492 <211> 21 <212> DNA <213> Brassica rapa <400> 492 ctgtttatcg gaccattgtt g 21 <210> 493 <211> 20 <212> DNA <213> Brassica rapa <400> 493 ttagtggcga tcctgagaag 20 <210> 494 <211> 20 <212> DNA <213> Brassica rapa <400> 494 atcatcgaca tcatcagcct 20 <210> 495 <211> 22 <212> DNA <213> Brassica rapa <400> 495 gctggttcca gctaatggtt ac 22 <210> 496 <211> 23 <212> DNA <213> Brassica rapa <400> 496 tgaatttatg agattcggat tgg 23 <210> 497 <211> 23 <212> DNA <213> Brassica rapa <400> 497 cacaaataaa ccgttaaaca tca 23 <210> 498 <211> 20 <212> DNA <213> Brassica rapa <400> 498 gagctgaaaa ggatatgcca 20 <210> 499 <211> 21 <212> DNA <213> Brassica rapa <400> 499 aacagtttct acgcatcgtg t 21 <210> 500 <211> 22 <212> DNA <213> Brassica rapa <400> 500 gcgttttcga ttttaaattt tg 22 <210> 501 <211> 20 <212> DNA <213> Brassica rapa <400> 501 tgtcgccatt ttactgttcg 20 <210> 502 <211> 20 <212> DNA <213> Brassica rapa <400> 502 attgcaatct tcctccaacg 20 <210> 503 <211> 20 <212> DNA <213> Brassica rapa <400> 503 gtggattttt gcattcttgg 20 <210> 504 <211> 20 <212> DNA <213> Brassica rapa <400> 504 gaaaaaggaa aaattgggga 20 <210> 505 <211> 22 <212> DNA <213> Brassica rapa <400> 505 gagaagaatt ttcccactat gc 22 <210> 506 <211> 27 <212> DNA <213> Brassica rapa <400> 506 gattttattg acaatgtata tttgctg 27 <210> 507 <211> 20 <212> DNA <213> Brassica rapa <400> 507 atcagtgccg gccttgaata 20 <210> 508 <211> 21 <212> DNA <213> Brassica rapa <400> 508 aagcttgagc ttccagcctt c 21 <210> 509 <211> 23 <212> DNA <213> Brassica rapa <400> 509 ttgaataatg accccaaata tca 23 <210> 510 <211> 26 <212> DNA <213> Brassica rapa <400> 510 tcaataggta ttaaccaatt ctaccg 26 <210> 511 <211> 21 <212> DNA <213> Brassica rapa <400> 511 agggagactg gtagagagga a 21 <210> 512 <211> 20 <212> DNA <213> Brassica rapa <400> 512 cgttcaggat ttttagggct 20 <110> Korea <120> Development of sequence-based microsatellite markers in Brassica          rapa and construction of genetic map <160> 512 <170> KopatentIn 1.71 <210> 1 <211> 20 <212> DNA <213> Brassica rapa <400> 1 ttctttggag atggtgtggt 20 <210> 2 <211> 20 <212> DNA <213> Brassica rapa <400> 2 caatcttgtg gtgagggaag 20 <210> 3 <211> 20 <212> DNA <213> Brassica rapa <400> 3 tctccatctc tcttccatcg 20 <210> 4 <211> 20 <212> DNA <213> Brassica rapa <400> 4 gtcctgcctg gtttgaataa 20 <210> 5 <211> 18 <212> DNA <213> Brassica rapa <400> 5 gttttaaatg ccgcgttg 18 <210> 6 <211> 19 <212> DNA <213> Brassica rapa <400> 6 gggatcaaga gatgtggga 19 <210> 7 <211> 21 <212> DNA <213> Brassica rapa <400> 7 tgatgaaaaa tgcatcacaa c 21 <210> 8 <211> 20 <212> DNA <213> Brassica rapa <400> 8 ttcatgtgga agcatttgag 20 <210> 9 <211> 20 <212> DNA <213> Brassica rapa <400> 9 cgggtcgtat tctctaacca 20 <210> 10 <211> 20 <212> DNA <213> Brassica rapa <400> 10 actcgccttc tccaactttt 20 <210> 11 <211> 20 <212> DNA <213> Brassica rapa <400> 11 gacagtggtt gaaaaagcca 20 <210> 12 <211> 20 <212> DNA <213> Brassica rapa <400> 12 cattcagtga ggtttcacca 20 <210> 13 <211> 20 <212> DNA <213> Brassica rapa <400> 13 gtcaacaacc tccgcgatag 20 <210> 14 <211> 20 <212> DNA <213> Brassica rapa <400> 14 agatcactca ccttcccggt 20 <210> 15 <211> 21 <212> DNA <213> Brassica rapa <400> 15 gaccttcggt tcagggtatg g 21 <210> 16 <211> 21 <212> DNA <213> Brassica rapa <400> 16 ctgaacggtc aatttgtttg g 21 <210> 17 <211> 20 <212> DNA <213> Brassica rapa <400> 17 tcaagcgcaa caaacattgg 20 <210> 18 <211> 24 <212> DNA <213> Brassica rapa <400> 18 tggtgttagg gtttaaggtt gtgg 24 <210> 19 <211> 20 <212> DNA <213> Brassica rapa <400> 19 caaaaagagc gttacctcca 20 <210> 20 <211> 20 <212> DNA <213> Brassica rapa <400> 20 gatgaagctc tgaagaccga 20 <210> 21 <211> 20 <212> DNA <213> Brassica rapa <400> 21 acctaccaac cccaaaaagg 20 <210> 22 <211> 20 <212> DNA <213> Brassica rapa <400> 22 gaagctttcg atgcggttta 20 <210> 23 <211> 21 <212> DNA <213> Brassica rapa <400> 23 ttaccgtgga tattggatga a 21 <210> 24 <211> 20 <212> DNA <213> Brassica rapa <400> 24 gccaggctaa gtgatgattc 20 <210> 25 <211> 22 <212> DNA <213> Brassica rapa <400> 25 ggatatctga tttgacccgt at 22 <210> 26 <211> 22 <212> DNA <213> Brassica rapa <400> 26 aaccgaaaac tccagataat ca 22 <210> 27 <211> 20 <212> DNA <213> Brassica rapa <400> 27 tgtgcttgcg ttttaaagga 20 <210> 28 <211> 20 <212> DNA <213> Brassica rapa <400> 28 gcaaaaccca caggtcagat 20 <210> 29 <211> 22 <212> DNA <213> Brassica rapa <400> 29 agaccagtcg gatctagagg at 22 <210> 30 <211> 22 <212> DNA <213> Brassica rapa <400> 30 caaccacatg agattggttt ag 22 <210> 31 <211> 22 <212> DNA <213> Brassica rapa <400> 31 ttctccccat cctctcatct ta 22 <210> 32 <211> 22 <212> DNA <213> Brassica rapa <400> 32 acccacaacc aacaaaatct tc 22 <210> 33 <211> 20 <212> DNA <213> Brassica rapa <400> 33 cggatgagag gatttacacg 20 <210> 34 <211> 20 <212> DNA <213> Brassica rapa <400> 34 cctcctggag cttatccatt 20 <210> 35 <211> 26 <212> DNA <213> Brassica rapa <400> 35 tcttcaagtc aaaatactca cattca 26 <210> 36 <211> 27 <212> DNA <213> Brassica rapa <400> 36 aaacgacaaa tacatatgac agtttta 27 <210> 37 <211> 20 <212> DNA <213> Brassica rapa <400> 37 ctatctcaga ccagcggaaa 20 <210> 38 <211> 20 <212> DNA <213> Brassica rapa <400> 38 ttgcataatc gtggaacgta 20 <210> 39 <211> 20 <212> DNA <213> Brassica rapa <400> 39 gagtgggatt ctcatcatgg 20 <210> 40 <211> 20 <212> DNA <213> Brassica rapa <400> 40 taccgtcttg gctcttgagt 20 <210> 41 <211> 25 <212> DNA <213> Brassica rapa <400> 41 tctttattca atttgctcac attcc 25 <210> 42 <211> 20 <212> DNA <213> Brassica rapa <400> 42 ttcgtggttt cgcggattat 20 <210> 43 <211> 20 <212> DNA <213> Brassica rapa <400> 43 tatcaagtgg ggacgaatgg 20 <210> 44 <211> 20 <212> DNA <213> Brassica rapa <400> 44 aaggaagaag ggtggaatcg 20 <210> 45 <211> 20 <212> DNA <213> Brassica rapa <400> 45 catcaacgtg gcatagtctg 20 <210> 46 <211> 20 <212> DNA <213> Brassica rapa <400> 46 caaatttctg tgttgcgttg 20 <210> 47 <211> 24 <212> DNA <213> Brassica rapa <400> 47 gcgccaatta taaatttgat tttc 24 <210> 48 <211> 20 <212> DNA <213> Brassica rapa <400> 48 tcctcctgaa cctggtcttg 20 <210> 49 <211> 23 <212> DNA <213> Brassica rapa <400> 49 ttgtgcacca atctattaca aaa 23 <210> 50 <211> 23 <212> DNA <213> Brassica rapa <400> 50 tgaagctgtt cttacaaaac aaa 23 <210> 51 <211> 22 <212> DNA <213> Brassica rapa <400> 51 tatagcctta gtggtagcga gg 22 <210> 52 <211> 22 <212> DNA <213> Brassica rapa <400> 52 ttttacccca agaatgtttg tc 22 <210> 53 <211> 20 <212> DNA <213> Brassica rapa <400> 53 aggggagatg gatctggtgt 20 <210> 54 <211> 20 <212> DNA <213> Brassica rapa <400> 54 tgagactcgc taggtttgcc 20 <210> 55 <211> 18 <212> DNA <213> Brassica rapa <400> 55 ggatcctccc atagctcg 18 <210> 56 <211> 18 <212> DNA <213> Brassica rapa <400> 56 cagtcgttgc gggataga 18 <210> 57 <211> 20 <212> DNA <213> Brassica rapa <400> 57 cgatttcgaa gtagccttca 20 <210> 58 <211> 20 <212> DNA <213> Brassica rapa <400> 58 tcaagaagca aatgaaaggg 20 <210> 59 <211> 18 <212> DNA <213> Brassica rapa <400> 59 tacgattgtg tgcatggc 18 <210> 60 <211> 20 <212> DNA <213> Brassica rapa <400> 60 aaccatctag accaccacga 20 <210> 61 <211> 19 <212> DNA <213> Brassica rapa <400> 61 tcacattatt ttgtcgccg 19 <210> 62 <211> 20 <212> DNA <213> Brassica rapa <400> 62 tcacaacatt tgaccgtttg 20 <210> 63 <211> 20 <212> DNA <213> Brassica rapa <400> 63 caccatgtga caaaacgaaa 20 <210> 64 <211> 21 <212> DNA <213> Brassica rapa <400> 64 ggacaaggat aatgatctcg g 21 <210> 65 <211> 20 <212> DNA <213> Brassica rapa <400> 65 tgatatcgaa aacaaatgcg 20 <210> 66 <211> 21 <212> DNA <213> Brassica rapa <400> 66 tgtctgcaac atcagtttca a 21 <210> 67 <211> 20 <212> DNA <213> Brassica rapa <400> 67 tgtttgaatc gtccattgtg 20 <210> 68 <211> 22 <212> DNA <213> Brassica rapa <400> 68 tggacgtaca tgcctactaa ca 22 <210> 69 <211> 20 <212> DNA <213> Brassica rapa <400> 69 ttgtcctctt ttcgaacctg 20 <210> 70 <211> 21 <212> DNA <213> Brassica rapa <400> 70 ttcctcgacc aattagtttc a 21 <210> 71 <211> 27 <212> DNA <213> Brassica rapa <400> 71 gctaaaggtt tagtccaaat aggattc 27 <210> 72 <211> 20 <212> DNA <213> Brassica rapa <400> 72 gcaaaatgat gccccataaa 20 <210> 73 <211> 20 <212> DNA <213> Brassica rapa <400> 73 tcgtttaccg gtcgtacgtt 20 <210> 74 <211> 20 <212> DNA <213> Brassica rapa <400> 74 gcctgagaga ccgtgagaga 20 <210> 75 <211> 23 <212> DNA <213> Brassica rapa <400> 75 agcacagtca aaactcagaa tga 23 <210> 76 <211> 20 <212> DNA <213> Brassica rapa <400> 76 aggcatgcgc tctctatctc 20 <210> 77 <211> 22 <212> DNA <213> Brassica rapa <400> 77 aaaagttggt cattcttctg ga 22 <210> 78 <211> 22 <212> DNA <213> Brassica rapa <400> 78 gtttatggtt acgggtggat aa 22 <210> 79 <211> 20 <212> DNA <213> Brassica rapa <400> 79 gacgacagct tcgaaagaga 20 <210> 80 <211> 20 <212> DNA <213> Brassica rapa <400> 80 tgagcattgt tttggtgatg 20 <210> 81 <211> 22 <212> DNA <213> Brassica rapa <400> 81 cgtatccatg gccttgaatt tt 22 <210> 82 <211> 20 <212> DNA <213> Brassica rapa <400> 82 ggcgagaacc ttgatgatcc 20 <210> 83 <211> 20 <212> DNA <213> Brassica rapa <400> 83 taatgccaca tcagcagaaa 20 <210> 84 <211> 20 <212> DNA <213> Brassica rapa <400> 84 acagcggtga aatgaactgt 20 <210> 85 <211> 18 <212> DNA <213> Brassica rapa <400> 85 gccgctggac acctaaat 18 <210> 86 <211> 19 <212> DNA <213> Brassica rapa <400> 86 catggtacgt cggttttga 19 <210> 87 <211> 21 <212> DNA <213> Brassica rapa <400> 87 cagtgaagtt caaccgcagt a 21 <210> 88 <211> 25 <212> DNA <213> Brassica rapa <400> 88 catgagtgaa cataaaacag tgaaa 25 <210> 89 <211> 22 <212> DNA <213> Brassica rapa <400> 89 catcactttt tcaggtggat tt 22 <210> 90 <211> 22 <212> DNA <213> Brassica rapa <400> 90 ctttctctct acctggtgat gg 22 <210> 91 <211> 19 <212> DNA <213> Brassica rapa <400> 91 tcctcgtgct tgtgaacag 19 <210> 92 <211> 19 <212> DNA <213> Brassica rapa <400> 92 tctgtttgtt ccgcagatg 19 <210> 93 <211> 22 <212> DNA <213> Brassica rapa <400> 93 tgttaccaca ggaaccgttc aa 22 <210> 94 <211> 20 <212> DNA <213> Brassica rapa <400> 94 cttgggcaca ccatcatctg 20 <210> 95 <211> 20 <212> DNA <213> Brassica rapa <400> 95 gctcatcctt ctcatgcagt 20 <210> 96 <211> 22 <212> DNA <213> Brassica rapa <400> 96 tggtattcgt acgttaaaca cg 22 <210> 97 <211> 20 <212> DNA <213> Brassica rapa <400> 97 ccctttccgt aaatccactt 20 <210> 98 <211> 20 <212> DNA <213> Brassica rapa <400> 98 tttgcggttg aaactcattt 20 <210> 99 <211> 20 <212> DNA <213> Brassica rapa <400> 99 atgtcatcaa gaccatcccg 20 <210> 100 <211> 20 <212> DNA <213> Brassica rapa <400> 100 gcatggcttg gtttcagaac 20 <210> 101 <211> 24 <212> DNA <213> Brassica rapa <400> 101 tggcattgac agagctagta agag 24 <210> 102 <211> 26 <212> DNA <213> Brassica rapa <400> 102 tttatttagt ttcataccct cgcagt 26 <210> 103 <211> 20 <212> DNA <213> Brassica rapa <400> 103 ccatgactcc attgtcgatt 20 <210> 104 <211> 20 <212> DNA <213> Brassica rapa <400> 104 cctgatcttc atgctggttc 20 <210> 105 <211> 20 <212> DNA <213> Brassica rapa <400> 105 ccccacccct taagtgctat 20 <210> 106 <211> 20 <212> DNA <213> Brassica rapa <400> 106 agagaggaag aaggagcgga 20 <210> 107 <211> 20 <212> DNA <213> Brassica rapa <400> 107 acaaaatttc tggccaatca 20 <210> 108 <211> 20 <212> DNA <213> Brassica rapa <400> 108 acggtggtgg ttagatgaga 20 <210> 109 <211> 20 <212> DNA <213> Brassica rapa <400> 109 tcgaggtggt tacaatccaa 20 <210> 110 <211> 20 <212> DNA <213> Brassica rapa <400> 110 caatgcggat ctacctctca 20 <210> 111 <211> 24 <212> DNA <213> Brassica rapa <400> 111 tcaagcatgt ccttaaaact ctga 24 <210> 112 <211> 20 <212> DNA <213> Brassica rapa <400> 112 gcgttcacgt ttcccatatc 20 <210> 113 <211> 22 <212> DNA <213> Brassica rapa <400> 113 gagaagaatt ttcccactat gc 22 <210> 114 <211> 27 <212> DNA <213> Brassica rapa <400> 114 gattttattg acaatgtata tttgctg 27 <210> 115 <211> 21 <212> DNA <213> Brassica rapa <400> 115 tgaaccctcg aaactgaaag a 21 <210> 116 <211> 20 <212> DNA <213> Brassica rapa <400> 116 ggacgttaac gaaggcaaaa 20 <210> 117 <211> 24 <212> DNA <213> Brassica rapa <400> 117 tttttgggtt tcttctcaaa atgc 24 <210> 118 <211> 20 <212> DNA <213> Brassica rapa <400> 118 actccagcga atttggcttt 20 <210> 119 <211> 20 <212> DNA <213> Brassica rapa <400> 119 tttgacatcg tgcaatgcta 20 <210> 120 <211> 20 <212> DNA <213> Brassica rapa <400> 120 ttgggctggt cctgaagata 20 <210> 121 <211> 22 <212> DNA <213> Brassica rapa <400> 121 actctttccc tctgcttcaa at 22 <210> 122 <211> 22 <212> DNA <213> Brassica rapa <400> 122 ttcaaaacga gagaggttag ga 22 <210> 123 <211> 22 <212> DNA <213> Brassica rapa <400> 123 ttcttgacca aaagaatcat gg 22 <210> 124 <211> 20 <212> DNA <213> Brassica rapa <400> 124 ctaacacggg gaaaagcaga 20 <210> 125 <211> 21 <212> DNA <213> Brassica rapa <400> 125 ccaaatgcag cagcgattaa a 21 <210> 126 <211> 19 <212> DNA <213> Brassica rapa <400> 126 cgggggttgc agaaagata 19 <210> 127 <211> 22 <212> DNA <213> Brassica rapa <400> 127 tcaacattgc catctaaaaa gg 22 <210> 128 <211> 22 <212> DNA <213> Brassica rapa <400> 128 tagactcatc aggttggctt ga 22 <210> 129 <211> 20 <212> DNA <213> Brassica rapa <400> 129 ttttcaactc ccacatgcac 20 <210> 130 <211> 21 <212> DNA <213> Brassica rapa <400> 130 tgggtatgtg ccaaattgtt t 21 <210> 131 <211> 21 <212> DNA <213> Brassica rapa <400> 131 ttccggtcac ttctagcttc a 21 <210> 132 <211> 24 <212> DNA <213> Brassica rapa <400> 132 ttttggtggt tagtatgtcg ctat 24 <210> 133 <211> 18 <212> DNA <213> Brassica rapa <133> 133 tatcaggaaa aggccacg 18 <210> 134 <211> 18 <212> DNA <213> Brassica rapa <400> 134 tcatcagcct tctctgcc 18 <210> 135 <211> 22 <212> DNA <213> Brassica rapa <400> 135 agctgctaaa gaggttgttg ac 22 <210> 136 <211> 22 <212> DNA <213> Brassica rapa <400> 136 aacatcagcc tgtcaagaga at 22 <210> 137 <211> 22 <212> DNA <213> Brassica rapa <400> 137 atgagaccgt gatgagaaat ga 22 <210> 138 <211> 22 <212> DNA <213> Brassica rapa <400> 138 gtgccacatc aacaagtagc at 22 <139> <211> 24 <212> DNA <213> Brassica rapa <400> 139 tcgaaccgaa gtaaataacg gact 24 <210> 140 <211> 20 <212> DNA <213> Brassica rapa <400> 140 tttcgcccac tgacgctatt 20 <210> 141 <211> 26 <212> DNA <213> Brassica rapa <400> 141 tcgatgatta gtttagttat ttcacg 26 <210> 142 <211> 22 <212> DNA <213> Brassica rapa <400> 142 cctcaaacca aggaagattt ca 22 <210> 143 <211> 23 <212> DNA <213> Brassica rapa <400> 143 ttgtttcttg aacatctcgt cca 23 <210> 144 <211> 26 <212> DNA <213> Brassica rapa <400> 144 tcaagtctac ctcttgatga cgaaaa 26 <210> 145 <211> 20 <212> DNA <213> Brassica rapa <400> 145 acttcaaaac ccgaaaagcc 20 <210> 146 <211> 20 <212> DNA <213> Brassica rapa <400> 146 gatctcagag gggagggaga 20 <210> 147 <211> 20 <212> DNA <213> Brassica rapa <400> 147 tcgctctcac accccttctc 20 <210> 148 <211> 20 <212> DNA <213> Brassica rapa <400> 148 gtggcaaatt cggacagacc 20 <210> 149 <211> 22 <212> DNA <213> Brassica rapa <400> 149 ggtcaggtgc tactcagact cc 22 <210> 150 <211> 21 <212> DNA <213> Brassica rapa <400> 150 ttgaagagga tccaccaaaa g 21 <210> 151 <211> 20 <212> DNA <213> Brassica rapa <400> 151 gggtttgatg gttattcgag 20 <210> 152 <211> 20 <212> DNA <213> Brassica rapa <400> 152 ttcacctatg cgatgctttt 20 <210> 153 <211> 20 <212> DNA <213> Brassica rapa <400> 153 ttcaagcgta gcctagcaca 20 <210> 154 <211> 20 <212> DNA <213> Brassica rapa <400> 154 acgtgcaact gagtttgtgg 20 <210> 155 <211> 20 <212> DNA <213> Brassica rapa <400> 155 ctaaatgcgg cctttttaca 20 <210> 156 <211> 20 <212> DNA <213> Brassica rapa <400> 156 tagccttctt gttcagccac 20 <210> 157 <211> 20 <212> DNA <213> Brassica rapa <400> 157 acgccagtag gcctatcaaa 20 <210> 158 <211> 20 <212> DNA <213> Brassica rapa <400> 158 aagccaacaa tgccagaatc 20 <210> 159 <211> 22 <212> DNA <213> Brassica rapa <400> 159 cccaaaacat ccaaactcct ca 22 <210> 160 <211> 24 <212> DNA <213> Brassica rapa <400> 160 ccccaaagag atacagaaca aagc 24 <210> 161 <211> 20 <212> DNA <213> Brassica rapa <400> 161 tggaacggga aaagacataa 20 <210> 162 <211> 20 <212> DNA <213> Brassica rapa <400> 162 tgcaaacatc atcattccat 20 <210> 163 <211> 20 <212> DNA <213> Brassica rapa <400> 163 aaaaacccca tgttttctca 20 <210> 164 <211> 20 <212> DNA <213> Brassica rapa <400> 164 gaagttgaag ggaaagaggg 20 <210> 165 <211> 18 <212> DNA <213> Brassica rapa <400> 165 atggatcatc ccgcttct 18 <210> 166 <211> 18 <212> DNA <213> Brassica rapa <400> 166 cagggaggga tcaagagg 18 <210> 167 <211> 20 <212> DNA <213> Brassica rapa <400> 167 gaaagatgag agcgctgtgt 20 <210> 168 <211> 20 <212> DNA <213> Brassica rapa <400> 168 atgacaacga ggagcgtaag 20 <210> 169 <211> 22 <212> DNA <213> Brassica rapa <400> 169 caacaaacgc atttctctta ca 22 <210> 170 <211> 20 <212> DNA <213> Brassica rapa <400> 170 ctcctcgaga ccgtagacct 20 <210> 171 <211> 20 <212> DNA <213> Brassica rapa <400> 171 gtgagtgcga cacgtatgaa 20 <210> 172 <211> 20 <212> DNA <213> Brassica rapa <400> 172 tctttgtgct tctcctggtc 20 <210> 173 <211> 20 <212> DNA <213> Brassica rapa <400> 173 aaaatctcgc ttctgcgctt 20 <210> 174 <211> 20 <212> DNA <213> Brassica rapa <400> 174 tccctcaccc tgtgcaatag 20 <175> 175 <211> 20 <212> DNA <213> Brassica rapa <400> 175 gccacgtcaa tcctacaaag 20 <210> 176 <211> 22 <212> DNA <213> Brassica rapa <400> 176 ggggatactt agatggtcag aa 22 <210> 177 <211> 20 <212> DNA <213> Brassica rapa <400> 177 ctgaaccgaa ttggctaaaa 20 <210> 178 <211> 20 <212> DNA <213> Brassica rapa <400> 178 taaacagggg aactgagctg 20 <210> 179 <211> 20 <212> DNA <213> Brassica rapa <400> 179 gaggccaatt cttcaacaga 20 <210> 180 <211> 20 <212> DNA <213> Brassica rapa <400> 180 tgcttcattc cggtattcat 20 <210> 181 <211> 22 <212> DNA <213> Brassica rapa <400> 181 tcatagcata cagtgcattt tt 22 <210> 182 <211> 21 <212> DNA <213> Brassica rapa <400> 182 tggtttcaag ttagaccgat g 21 <210> 183 <211> 20 <212> DNA <213> Brassica rapa <400> 183 gttgctttta tgctgccgac 20 <210> 184 <211> 20 <212> DNA <213> Brassica rapa <400> 184 ggatttgtca aacgcgagac 20 <210> 185 <211> 20 <212> DNA <213> Brassica rapa <400> 185 tgcaaatcca agaaacaaaa 20 <210> 186 <211> 20 <212> DNA <213> Brassica rapa <400> 186 aagaggtgca agttccactg 20 <210> 187 <211> 20 <212> DNA <213> Brassica rapa <400> 187 attccggttt atctttcggc 20 <210> 188 <211> 20 <212> DNA <213> Brassica rapa <400> 188 ttgcgaaagt tcaaatgcct 20 <210> 189 <211> 22 <212> DNA <213> Brassica rapa <400> 189 gatgaaagta actggttgca ca 22 <210> 190 <211> 21 <212> DNA <213> Brassica rapa <400> 190 cggaaatcag gtcaatgtat g 21 <210> 191 <211> 22 <212> DNA <213> Brassica rapa <400> 191 tgaacctgtt tcatccaaga ga 22 <210> 192 <211> 22 <212> DNA <213> Brassica rapa <400> 192 tctggcatga tgtagttgct tt 22 <210> 193 <211> 20 <212> DNA <213> Brassica rapa <400> 193 cggagataac cggaatggaa 20 <210> 194 <211> 21 <212> DNA <213> Brassica rapa <400> 194 ggatgctctg agacacccaa a 21 <210> 195 <211> 24 <212> DNA <213> Brassica rapa <400> 195 tgaaaatcaa cacgaacaca caga 24 <210> 196 <211> 20 <212> DNA <213> Brassica rapa <400> 196 ctcgtggggg aatgagtgag 20 <210> 197 <211> 24 <212> DNA <213> Brassica rapa <400> 197 cgacggatct gaagagagta gagg 24 <210> 198 <211> 22 <212> DNA <213> Brassica rapa <400> 198 cgaaacagag gaaacatcac cc 22 <210> 199 <211> 22 <212> DNA <213> Brassica rapa <400> 199 gaagaagacg aagttgagct gt 22 <210> 200 <211> 22 <212> DNA <213> Brassica rapa <400> 200 gcccatattg atacttaacg ga 22 <210> 201 <211> 21 <212> DNA <213> Brassica rapa <400> 201 aagcttgagc ttccagcctt c 21 <210> 202 <211> 20 <212> DNA <213> Brassica rapa <400> 202 atcagtgccg gccttgaata 20 <210> 203 <211> 24 <212> DNA <213> Brassica rapa <400> 203 ttgaaataca tgatacccca acca 24 <210> 204 <211> 20 <212> DNA <213> Brassica rapa <400> 204 ccgttttcag ggcacagttt 20 <210> 205 <211> 18 <212> DNA <213> Brassica rapa <400> 205 agtggtcccc acgagaat 18 <206> 206 <211> 18 <212> DNA <213> Brassica rapa <400> 206 tcgagaaggt cggtgaga 18 <210> 207 <211> 20 <212> DNA <213> Brassica rapa <400> 207 tcatcagctg cacgaaagta 20 <210> 208 <211> 20 <212> DNA <213> Brassica rapa <400> 208 atgaagaatg gtgcatggat 20 <210> 209 <211> 22 <212> DNA <213> Brassica rapa <400> 209 tcggttaaat cgagtaacga tg 22 <210> 210 <211> 23 <212> DNA <213> Brassica rapa <400> 210 tttcaggacc tagacgttac caa 23 <210> 211 <211> 22 <212> DNA <213> Brassica rapa <400> 211 tcattggtga agaacccaca aa 22 <210> 212 <211> 24 <212> DNA <213> Brassica rapa <400> 212 gcgaccataa aaagagagtg agaa 24 <210> 213 <211> 21 <212> DNA <213> Brassica rapa <400> 213 gtccatctcc tacctgctcc a 21 <210> 214 <211> 22 <212> DNA <213> Brassica rapa <400> 214 gttttgagcc gaataatggt tg 22 <210> 215 <211> 22 <212> DNA <213> Brassica rapa <400> 215 cgatttggac aatgactagt gg 22 <210> 216 <211> 20 <212> DNA <213> Brassica rapa <400> 216 aacgccatgg aaacagaaac 20 <210> 217 <211> 20 <212> DNA <213> Brassica rapa <400> 217 tccaaatcca actcttccca 20 <210> 218 <211> 20 <212> DNA <213> Brassica rapa <400> 218 ccccatttgg aagatgctaa 20 <210> 219 <211> 20 <212> DNA <213> Brassica rapa <400> 219 gcagcaaaac ctctcccttt 20 <210> 220 <211> 20 <212> DNA <213> Brassica rapa <400> 220 ccttcagagg tgcgtttgac 20 <210> 221 <211> 20 <212> DNA <213> Brassica rapa <400> 221 aaacgtggaa gcacattttt 20 <210> 222 <211> 20 <212> DNA <213> Brassica rapa <400> 222 agtcacgacg tgtttcatgt 20 <210> 223 <211> 20 <212> DNA <213> Brassica rapa <400> 223 aatttttaga aggaggcgga 20 <210> 224 <211> 20 <212> DNA <213> Brassica rapa <400> 224 caccatcaca acaacaagga 20 <210> 225 <211> 20 <212> DNA <213> Brassica rapa <400> 225 aaatctccca acatggttca 20 <210> 226 <211> 22 <212> DNA <213> Brassica rapa <400> 226 agatgccatt actcctaact tg 22 <210> 227 <211> 20 <212> DNA <213> Brassica rapa <400> 227 cagacggttg catgaagata 20 <210> 228 <211> 22 <212> DNA <213> Brassica rapa <400> 228 agatgtgggt aattgcagtt tt 22 <210> 229 <211> 25 <212> DNA <213> Brassica rapa <400> 229 cccaaatacg aaaacaaagt ttgac 25 <210> 230 <211> 20 <212> DNA <213> Brassica rapa <400> 230 aggatctcat ccgctttcca 20 <210> 231 <211> 25 <212> DNA <213> Brassica rapa <400> 231 aaaaagaaat ggatattgtg tgaaa 25 <210> 232 <211> 20 <212> DNA <213> Brassica rapa <400> 232 cctggatcaa gaccacgaag 20 <210> 233 <211> 22 <212> DNA <213> Brassica rapa <400> 233 tgcttttaac caaactcaaa cg 22 <210> 234 <211> 20 <212> DNA <213> Brassica rapa <400> 234 tgcagagagg caagtttcaa 20 <210> 235 <211> 24 <212> DNA <213> Brassica rapa <400> 235 tgcatgatgt tcatgtcttg taaa 24 <210> 236 <211> 25 <212> DNA <213> Brassica rapa <400> 236 tccttctgta aaccggttgt aattt 25 <210> 237 <211> 20 <212> DNA <213> Brassica rapa <400> 237 tcgtttgacc aaccgtacaa 20 <210> 238 <211> 20 <212> DNA <213> Brassica rapa <400> 238 cttgccagcg ttgatacaga 20 <210> 239 <211> 25 <212> DNA <213> Brassica rapa <400> 239 tgttcatgtt catcagccct ctcaa 25 <210> 240 <211> 25 <212> DNA <213> Brassica rapa <400> 240 tcttttctgc cctcacctac aatca 25 <210> 241 <211> 20 <212> DNA <213> Brassica rapa <400> 241 tcgggattgc tatcaacagt 20 <210> 242 <211> 20 <212> DNA <213> Brassica rapa <400> 242 agctctcaag cgaaattcca 20 <210> 243 <211> 20 <212> DNA <213> Brassica rapa <400> 243 ctcctggaaa gaacaccaca 20 <210> 244 <211> 20 <212> DNA <213> Brassica rapa <400> 244 aaagcacttg agccagcttc 20 <210> 245 <211> 18 <212> DNA <213> Brassica rapa <400> 245 gggctcttcc aacaggtc 18 <210> 246 <211> 18 <212> DNA <213> Brassica rapa <400> 246 tgtgagatcg ccgagagt 18 <210> 247 <211> 27 <212> DNA <213> Brassica rapa <400> 247 aaccttcatt tcatatacat acacaca 27 <210> 248 <211> 24 <212> DNA <213> Brassica rapa <400> 248 ttcaatcatt tttattggtc atca 24 <210> 249 <211> 19 <212> DNA <213> Brassica rapa <400> 249 gcgcaaaacg catatctta 19 <210> 250 <211> 23 <212> DNA <213> Brassica rapa <400> 250 tcttttgatc ctttgtttct tca 23 <210> 251 <211> 23 <212> DNA <213> Brassica rapa <400> 251 ttgaaatgcg acttttatat gac 23 <210> 252 <211> 20 <212> DNA <213> Brassica rapa <400> 252 ccggttcaaa atatgacaca 20 <210> 253 <211> 17 <212> DNA <213> Brassica rapa <400> 253 cgcagccttt tgcttct 17 <210> 254 <211> 18 <212> DNA <213> Brassica rapa <400> 254 ttgctctcct gcagcttg 18 <210> 255 <211> 18 <212> DNA <213> Brassica rapa <400> 255 cgataccaac gcaccact 18 <210> 256 <211> 19 <212> DNA <213> Brassica rapa <400> 256 caacctccac atttttcca 19 <210> 257 <211> 24 <212> DNA <213> Brassica rapa <400> 257 tgctttaatc aatccaaatt cgtt 24 <210> 258 <211> 26 <212> DNA <213> Brassica rapa <400> 258 ttgtaccgag ttaagctgaa aaattc 26 <210> 259 <211> 22 <212> DNA <213> Brassica rapa <400> 259 gagatcacga aagctgtaga gg 22 <210> 260 <211> 22 <212> DNA <213> Brassica rapa <400> 260 cgaacttgaa agccagtaat tc 22 <210> 261 <211> 20 <212> DNA <213> Brassica rapa <400> 261 gaggaattaa cggcgtcttg 20 <210> 262 <211> 20 <212> DNA <213> Brassica rapa <400> 262 cagtcgccac tacctggttt 20 <210> 263 <211> 22 <212> DNA <213> Brassica rapa <400> 263 ctgttgaaaa cacagcacaa gt 22 <210> 264 <211> 22 <212> DNA <213> Brassica rapa <400> 264 ggtcgatctg ccatgtatta ct 22 <210> 265 <211> 27 <212> DNA <213> Brassica rapa <400> 265 tgtgttaaac tatctccgaa ctagaaa 27 <210> 266 <211> 27 <212> DNA <213> Brassica rapa <400> 266 tttctctagg atattagaaa aggaaaa 27 <210> 267 <211> 20 <212> DNA <213> Brassica rapa <400> 267 acttggagct cttggtatcg 20 <210> 268 <211> 19 <212> DNA <213> Brassica rapa <400> 268 aaacccttaa acctcgtcg 19 <210> 269 <211> 23 <212> DNA <213> Brassica rapa <400> 269 tcctaattga agccttggtc ata 23 <210> 270 <211> 23 <212> DNA <213> Brassica rapa <400> 270 cacttggcag aagaagagaa aaa 23 <210> 271 <211> 20 <212> DNA <213> Brassica rapa <400> 271 gattggcctg agacagaaga 20 <210> 272 <211> 20 <212> DNA <213> Brassica rapa <400> 272 ccagagcgcg aactctacta 20 <210> 273 <211> 20 <212> DNA <213> Brassica rapa <400> 273 catgtcaaac caacgaaaca 20 <210> 274 <211> 20 <212> DNA <213> Brassica rapa <400> 274 gatgaacttc gtgatttggg 20 <210> 275 <211> 20 <212> DNA <213> Brassica rapa <400> 275 gatacacaag gttcaacgcc 20 <210> 276 <211> 20 <212> DNA <213> Brassica rapa <400> 276 ttccttagct tgtgactggc 20 <210> 277 <211> 20 <212> DNA <213> Brassica rapa <400> 277 atcagaaccg aatccgacca 20 <210> 278 <211> 23 <212> DNA <213> Brassica rapa <400> 278 caatggttgc aatgttattt gga 23 <210> 279 <211> 27 <212> DNA <213> Brassica rapa <400> 279 cgagtttttg tgtgtacgta tagtaat 27 <210> 280 <211> 20 <212> DNA <213> Brassica rapa <400> 280 ccaaagtgcg taaaggaagg 20 <210> 281 <211> 26 <212> DNA <213> Brassica rapa <400> 281 ttatcaacat attttcaatc attcca 26 <210> 282 <211> 26 <212> DNA <213> Brassica rapa <400> 282 gctttggact atgcttctaa agtacg 26 <210> 283 <211> 23 <212> DNA <213> Brassica rapa <400> 283 tgtaaagttg tgcaaggatt gtg 23 <210> 284 <211> 24 <212> DNA <213> Brassica rapa <400> 284 tgggtttgtg aaaatatggt gaaa 24 <210> 285 <211> 20 <212> DNA <213> Brassica rapa <400> 285 ggagacgttg tcttcacctg 20 <210> 286 <211> 20 <212> DNA <213> Brassica rapa <400> 286 gttggggaga accagcttat 20 <210> 287 <211> 21 <212> DNA <213> Brassica rapa <400> 287 tttgatcgtc tttttcgttt g 21 <210> 288 <211> 20 <212> DNA <213> Brassica rapa <400> 288 gagcgggtag gttactggtt 20 <210> 289 <211> 21 <212> DNA <213> Brassica rapa <400> 289 gatgtgaggg taagtttgtg g 21 <210> 290 <211> 22 <212> DNA <213> Brassica rapa <400> 290 caatgaatgt gatgttatcc ga 22 <210> 291 <211> 20 <212> DNA <213> Brassica rapa <400> 291 gcatgatttt tgacaggctt 20 <210> 292 <211> 20 <212> DNA <213> Brassica rapa <400> 292 tgtcacgcgt cattcataga 20 <210> 293 <211> 20 <212> DNA <213> Brassica rapa <400> 293 ttcattctcc ttttgcgttc 20 <210> 294 <211> 20 <212> DNA <213> Brassica rapa <400> 294 cgcgtttcac ttcttcttgt 20 <210> 295 <211> 20 <212> DNA <213> Brassica rapa <400> 295 accattgaca gaagctggag 20 <210> 296 <211> 20 <212> DNA <213> Brassica rapa <400> 296 ttgcatcctt ttgtcacgta 20 <210> 297 <211> 23 <212> DNA <213> Brassica rapa <400> 297 cgagattttc gtataacgat tca 23 <210> 298 <211> 22 <212> DNA <213> Brassica rapa <400> 298 aaatcagact cactcgacca tt 22 <210> 299 <211> 22 <212> DNA <213> Brassica rapa <400> 299 aaagggagtt gaagtaagag gc 22 <210> 300 <211> 22 <212> DNA <213> Brassica rapa <400> 300 ctggacaagt gttctagtgc aa 22 <210> 301 <211> 26 <212> DNA <213> Brassica rapa <400> 301 gagagaagaa gaagaaggaa gcagaa 26 <210> 302 <211> 19 <212> DNA <213> Brassica rapa <400> 302 gatctcgcgt gtggcaact 19 <210> 303 <211> 22 <212> DNA <213> Brassica rapa <400> 303 gctaagaaag gagacaacat gg 22 <210> 304 <211> 22 <212> DNA <213> Brassica rapa <400> 304 gaaccttctg gttgaatgtg tt 22 <210> 305 <211> 27 <212> DNA <213> Brassica rapa <400> 305 gaaacaaatt atttaaaaat cagacca 27 <210> 306 <211> 23 <212> DNA <213> Brassica rapa <400> 306 tggaacaatc cgtaaaacta tgc 23 <210> 307 <211> 20 <212> DNA <213> Brassica rapa <400> 307 ataagggcat cgcctcaaca 20 <210> 308 <211> 20 <212> DNA <213> Brassica rapa <400> 308 tgcacgcatc cacataaaca 20 <210> 309 <211> 21 <212> DNA <213> Brassica rapa <400> 309 ggaccgactt tagcaagtcc a 21 <210> 310 <211> 27 <212> DNA <213> Brassica rapa <400> 310 gggtagctta gaagatcatc tctttgg 27 <210> 311 <211> 20 <212> DNA <213> Brassica rapa <400> 311 aggccaccac gtgattctaa 20 <210> 312 <211> 20 <212> DNA <213> Brassica rapa <400> 312 cacatatgtc cccttttcgc 20 <210> 313 <211> 20 <212> DNA <213> Brassica rapa <400> 313 tcttgcgaat gttccggttt 20 <210> 314 <211> 26 <212> DNA <213> Brassica rapa <400> 314 ccaaaattaa gagaataaat gattgg 26 <210> 315 <211> 20 <212> DNA <213> Brassica rapa <400> 315 tgatgtcgaa gaggctggat 20 <210> 316 <211> 20 <212> DNA <213> Brassica rapa <400> 316 gactcaaacc ctgtggacga 20 <210> 317 <211> 22 <212> DNA <213> Brassica rapa <400> 317 gagaaatcgc cacatatagc tc 22 <210> 318 <211> 22 <212> DNA <213> Brassica rapa <400> 318 gatttcaaaa tgcaagaagg tc 22 <210> 319 <211> 20 <212> DNA <213> Brassica rapa <400> 319 ctgcgcgtca gtcactacaa 20 <210> 320 <211> 20 <212> DNA <213> Brassica rapa <400> 320 agtctaccca aatgcggaca 20 <210> 321 <211> 20 <212> DNA <213> Brassica rapa <400> 321 ccaaggatga tggaggcata 20 <210> 322 <211> 20 <212> DNA <213> Brassica rapa <400> 322 tgactttcca tttccgtcgt 20 <210> 323 <211> 19 <212> DNA <213> Brassica rapa <400> 323 cgaacgaaca gaaacaagc 19 <210> 324 <211> 18 <212> DNA <213> Brassica rapa <400> 324 aacgaggcaa gggctaaa 18 <210> 325 <211> 19 <212> DNA <213> Brassica rapa <400> 325 ctggaccctg ccacacaag 19 <210> 326 <211> 22 <212> DNA <213> Brassica rapa <400> 326 gagtaggagt ttccgacgag ga 22 <210> 327 <211> 21 <212> DNA <213> Brassica rapa <400> 327 aactggaaac tccttggcac t 21 <210> 328 <211> 20 <212> DNA <213> Brassica rapa <400> 328 gtccaacttg atgcatttcg 20 <210> 329 <211> 22 <212> DNA <213> Brassica rapa <400> 329 aattcgtctt gcgtcggtaa aa 22 <210> 330 <211> 22 <212> DNA <213> Brassica rapa <400> 330 gcctagagat aaacgtggac cg 22 <210> 331 <211> 22 <212> DNA <213> Brassica rapa <400> 331 cagagcaatc gaagagaaga at 22 <210> 332 <211> 22 <212> DNA <213> Brassica rapa <400> 332 ttaggtgggt ctcaaatcaa at 22 <210> 333 <211> 20 <212> DNA <213> Brassica rapa <400> 333 cctatgtctc agccgtccat 20 <210> 334 <211> 20 <212> DNA <213> Brassica rapa <400> 334 taccaccgcc attaccattt 20 <210> 335 <211> 22 <212> DNA <213> Brassica rapa <400> 335 aaagattggg ttgtgagatt tg 22 <210> 336 <211> 22 <212> DNA <213> Brassica rapa <400> 336 atgttcgatt gcttctgaaa ct 22 <210> 337 <211> 19 <212> DNA <213> Brassica rapa <400> 337 ggaacgagtt gaacaccaa 19 <210> 338 <211> 18 <212> DNA <213> Brassica rapa <400> 338 ccgtcaaacc aatctcca 18 <210> 339 <211> 20 <212> DNA <213> Brassica rapa <400> 339 gccatgtggc cttctaaaaa 20 <210> 340 <211> 20 <212> DNA <213> Brassica rapa <400> 340 cgattcctgg tcatttggat 20 <210> 341 <211> 22 <212> DNA <213> Brassica rapa <400> 341 tcggttagag atctggagaa ga 22 <210> 342 <211> 22 <212> DNA <213> Brassica rapa <400> 342 aaaaatcaaa gtggcagcta aa 22 <210> 343 <211> 20 <212> DNA <213> Brassica rapa <400> 343 acgtggcatt cattaaacgg 20 <210> 344 <211> 22 <212> DNA <213> Brassica rapa <400> 344 gaaagagaga tccttcagcc aa 22 <210> 345 <211> 25 <212> DNA <213> Brassica rapa <400> 345 ttggatcaat caaactaaac cctga 25 <210> 346 <211> 20 <212> DNA <213> Brassica rapa <400> 346 ccaaaatgcc aacaaaagca 20 <210> 347 <211> 21 <212> DNA <213> Brassica rapa <400> 347 gggatattga agacccgcaa a 21 <210> 348 <211> 20 <212> DNA <213> Brassica rapa <400> 348 tctcccggtg gcttaaagaa 20 <210> 349 <211> 22 <212> DNA <213> Brassica rapa <400> 349 ttaatcatct gatgtcctcg aa 22 <210> 350 <211> 22 <212> DNA <213> Brassica rapa <400> 350 aacaacatca gttatcatgc ca 22 <210> 351 <211> 20 <212> DNA <213> Brassica rapa <400> 351 agttggattc tcgttgggaa 20 <210> 352 <211> 21 <212> DNA <213> Brassica rapa <352> 352 tgagaggtcc accttttgtc a 21 <210> 353 <211> 20 <212> DNA <213> Brassica rapa <400> 353 cgccctaaag tccggtagat 20 <210> 354 <211> 20 <212> DNA <213> Brassica rapa <400> 354 caaagcaagc gtaagaggga 20 <210> 355 <211> 20 <212> DNA <213> Brassica rapa <400> 355 tgggtaaaca aatggaggtg 20 <210> 356 <211> 20 <212> DNA <213> Brassica rapa <400> 356 ctcatctttc ccctccaaac 20 <210> 357 <211> 22 <212> DNA <213> Brassica rapa <400> 357 acgtaataat gttggttctc cg 22 <210> 358 <211> 22 <212> DNA <213> Brassica rapa <400> 358 tgattctcct tctccactgt ct 22 <210> 359 <211> 21 <212> DNA <213> Brassica rapa <400> 359 caagcaatca gagctttttg a 21 <210> 360 <211> 20 <212> DNA <213> Brassica rapa <400> 360 cagtctgtta tggtgtgcca 20 <210> 361 <211> 22 <212> DNA <213> Brassica rapa <400> 361 caaactctcc ttccacatgt ca 22 <210> 362 <211> 22 <212> DNA <213> Brassica rapa <400> 362 ttcttttaag taacctagtt ag 22 <210> 363 <211> 20 <212> DNA <213> Brassica rapa <400> 363 agaaagggaa atcaggaccg 20 <210> 364 <211> 20 <212> DNA <213> Brassica rapa <400> 364 caggctttac attccggttg 20 <210> 365 <211> 20 <212> DNA <213> Brassica rapa <400> 365 ccaacaacaa ccatggtgaa 20 <210> 366 <211> 20 <212> DNA <213> Brassica rapa <400> 366 caaactggag caaagcatca 20 <210> 367 <211> 20 <212> DNA <213> Brassica rapa <400> 367 ccactaccaa gccattgtga 20 <210> 368 <211> 20 <212> DNA <213> Brassica rapa <400> 368 gagaccacgc aagatcaggt 20 <210> 369 <211> 26 <212> DNA <213> Brassica rapa <400> 369 aattagcgcg ataacataaa taaaaa 26 <210> 370 <211> 27 <212> DNA <213> Brassica rapa <400> 370 aaattgctta actattaaaa ctgcaaa 27 <210> 371 <211> 20 <212> DNA <213> Brassica rapa <400> 371 cgagatgagc ttcgaacaat 20 <210> 372 <211> 20 <212> DNA <213> Brassica rapa <400> 372 aaaaagcgag ttgggaaaag 20 <210> 373 <211> 20 <212> DNA <213> Brassica rapa <400> 373 agccgttgca gatcatctta 20 <210> 374 <211> 25 <212> DNA <213> Brassica rapa <400> 374 tcgattaacc taagatgaag acagg 25 <210> 375 <211> 20 <212> DNA <213> Brassica rapa <400> 375 tgtcgccatt ttactgttcg 20 <210> 376 <211> 20 <212> DNA <213> Brassica rapa <400> 376 ggaaagaagg ttgtggcaaa 20 <210> 377 <211> 20 <212> DNA <213> Brassica rapa <400> 377 gttttgtggc cttcgagttt 20 <210> 378 <211> 26 <212> DNA <213> Brassica rapa <400> 378 tttggagaca tacaaatcgt taagag 26 <210> 379 <211> 20 <212> DNA <213> Brassica rapa <400> 379 aaactaggta aggaacccgc 20 <210> 380 <211> 20 <212> DNA <213> Brassica rapa <400> 380 aaacgaacaa ttgcaagtcc 20 <210> 381 <211> 20 <212> DNA <213> Brassica rapa <400> 381 gttgctgggc ttgcagttat 20 <210> 382 <211> 20 <212> DNA <213> Brassica rapa <400> 382 gagcgtacca gcaacctctc 20 <210> 383 <211> 22 <212> DNA <213> Brassica rapa <400> 383 ccaagaaatt ggtcatgatt tt 22 <210> 384 <211> 22 <212> DNA <213> Brassica rapa <400> 384 aatgcgtttt gtgtagcact tt 22 <210> 385 <211> 23 <212> DNA <213> Brassica rapa <400> 385 tttttggagc acacatctaa caa 23 <210> 386 <211> 25 <212> DNA <213> Brassica rapa <400> 386 tgggaaatat tcctaatctc agtca 25 <210> 387 <211> 22 <212> DNA <213> Brassica rapa <400> 387 gaggaagtac ggcaagaaac ca 22 <210> 388 <211> 22 <212> DNA <213> Brassica rapa <400> 388 aggacacatg tccacatgaa aa 22 <210> 389 <211> 20 <212> DNA <213> Brassica rapa <400> 389 tttcggtttg gtttggtttc 20 <210> 390 <211> 27 <212> DNA <213> Brassica rapa <400> 390 caaactagtt ggattcacaa ttataca 27 <210> 391 <211> 18 <212> DNA <213> Brassica rapa <400> 391 tggagaaggg gtctccat 18 <210> 392 <211> 21 <212> DNA <213> Brassica rapa <400> 392 ttgttcctaa caaacggaaa a 21 <210> 393 <211> 20 <212> DNA <213> Brassica rapa <400> 393 gtcgtcaggc aaaggtggtt 20 <210> 394 <211> 20 <212> DNA <213> Brassica rapa <400> 394 ggcggcagaa agaaagaaag 20 <210> 395 <211> 24 <212> DNA <213> Brassica rapa <400> 395 acacctactt gtttccatcc aaat 24 <210> 396 <211> 20 <212> DNA <213> Brassica rapa <400> 396 cgggtatttg cgttgtttcc 20 <210> 397 <211> 22 <212> DNA <213> Brassica rapa <400> 397 agtcggagga aacgcgaaat ta 22 <210> 398 <211> 27 <212> DNA <213> Brassica rapa <400> 398 cgaaataaag acagacagag acatcca 27 <210> 399 <211> 20 <212> DNA <213> Brassica rapa <400> 399 tcgcgaggaa tagaacgaat 20 <210> 400 <211> 21 <212> DNA <213> Brassica rapa <400> 400 ggaaccttct cgatggtttt t 21 <210> 401 <211> 25 <212> DNA <213> Brassica rapa <400> 401 ttcatccact ttgttcaata caaga 25 <210> 402 <211> 23 <212> DNA <213> Brassica rapa <400> 402 ccgtcaaaaa cgaagtaaat cac 23 <210> 403 <211> 20 <212> DNA <213> Brassica rapa <400> 403 tgtgtcgctc gtctacgtct 20 <210> 404 <211> 20 <212> DNA <213> Brassica rapa <400> 404 accatcgact tcgtggaaac 20 <210> 405 <211> 20 <212> DNA <213> Brassica rapa <400> 405 gccgactcct agtgaggaaa 20 <210> 406 <211> 20 <212> DNA <213> Brassica rapa <400> 406 tgtgttttgg gctcaaaggt 20 <210> 407 <211> 22 <212> DNA <213> Brassica rapa <400> 407 ctgctccttc acgttttcat ca 22 <210> 408 <211> 22 <212> DNA <213> Brassica rapa <400> 408 acggacagca acaacaacaa ga 22 <210> 409 <211> 20 <212> DNA <213> Brassica rapa <400> 409 ggagcttgtt caatcctcca 20 <210> 410 <211> 20 <212> DNA <213> Brassica rapa <400> 410 tgtacctgcg gcttctttct 20 <210> 411 <211> 23 <212> DNA <213> Brassica rapa <400> 411 gcctgcacta aaatagctgc aaa 23 <210> 412 <211> 20 <212> DNA <213> Brassica rapa <400> 412 gaatggaagg cgtcgatcat 20 <210> 413 <211> 20 <212> DNA <213> Brassica rapa <400> 413 ttttggagat tagtgtgccc 20 <210> 414 <211> 20 <212> DNA <213> Brassica rapa <400> 414 agtaaggcag agcagtgtgc 20 <210> 415 <211> 18 <212> DNA <213> Brassica rapa <400> 415 tccacgcaaa accaaaac 18 <210> 416 <211> 18 <212> DNA <213> Brassica rapa <400> 416 tcatcccctc cgttcttt 18 <210> 417 <211> 20 <212> DNA <213> Brassica rapa <400> 417 gggccaagtt acatggaaaa 20 <210> 418 <211> 20 <212> DNA <213> Brassica rapa <400> 418 gaaggaggat gagagccgtt 20 <210> 419 <211> 20 <212> DNA <213> Brassica rapa <400> 419 caatcgtttt acatgagccc 20 <210> 420 <211> 20 <212> DNA <213> Brassica rapa <400> 420 ccaagtgctt agtggttggt 20 <210> 421 <211> 26 <212> DNA <213> Brassica rapa <400> 421 tctttcttga aaagatttgg ttccat 26 <210> 422 <211> 21 <212> DNA <213> Brassica rapa <400> 422 tgggcattta gctacgacct t 21 <210> 423 <211> 20 <212> DNA <213> Brassica rapa <400> 423 tggatgttgt ttggttgatg 20 <210> 424 <211> 20 <212> DNA <213> Brassica rapa <400> 424 tgtccttttc tcaattccca 20 <210> 425 <211> 22 <212> DNA <213> Brassica rapa <400> 425 tgccaatctc agatcttttt ca 22 <210> 426 <211> 20 <212> DNA <213> Brassica rapa <400> 426 tctctcctat agccgccatc 20 <210> 427 <211> 22 <212> DNA <213> Brassica rapa <400> 427 gccacaacaa agtattgtct ca 22 <210> 428 <211> 23 <212> DNA <213> Brassica rapa <400> 428 aacatctatc ttactggaac gga 23 <210> 429 <211> 20 <212> DNA <213> Brassica rapa <400> 429 cggtgaccag taagctgttt 20 <210> 430 <211> 20 <212> DNA <213> Brassica rapa <400> 430 tgtctgtttc cacgtcaatg 20 <210> 431 <211> 21 <212> DNA <213> Brassica rapa <400> 431 ttcgattgtg gttcttattg g 21 <210> 432 <211> 23 <212> DNA <213> Brassica rapa <400> 432 ccatatttat cttagttctc gcc 23 <210> 433 <211> 21 <212> DNA <213> Brassica rapa <400> 433 aatgagtaat gtcccacacg a 21 <210> 434 <211> 21 <212> DNA <213> Brassica rapa <400> 434 tgaaattgcg gattctttag c 21 <210> 435 <211> 23 <212> DNA <213> Brassica rapa <400> 435 tcatctaaaa gatcaataag cga 23 <210> 436 <211> 20 <212> DNA <213> Brassica rapa <400> 436 agtcggatca tcctcggtat 20 <210> 437 <211> 22 <212> DNA <213> Brassica rapa <400> 437 ctctcgtctc ggaggatcta aa 22 <210> 438 <211> 22 <212> DNA <213> Brassica rapa <400> 438 gtgagagtgg ttgctgagtg ag 22 <210> 439 <211> 20 <212> DNA <213> Brassica rapa <400> 439 tgggtaattt gggaatttga 20 <210> 440 <211> 20 <212> DNA <213> Brassica rapa <400> 440 attgtacgtt gacgatggct 20 <210> 441 <211> 22 <212> DNA <213> Brassica rapa <400> 441 acctctgact gaaaatgaag tt 22 <210> 442 <211> 21 <212> DNA <213> Brassica rapa <400> 442 tgcatgtact ccctatgttc a 21 <210> 443 <211> 23 <212> DNA <213> Brassica rapa <400> 443 aacacttgat tttagaaact gga 23 <210> 444 <211> 22 <212> DNA <213> Brassica rapa <400> 444 ttgtagaact tgtgtgattg gg 22 <210> 445 <211> 20 <212> DNA <213> Brassica rapa <400> 445 tgccaacaaa tcaaggatgc 20 <210> 446 <211> 24 <212> DNA <213> Brassica rapa <400> 446 ccgaagttca cttgttattc caac 24 <210> 447 <211> 20 <212> DNA <213> Brassica rapa <400> 447 agaaagcatg cgtgagaaac 20 <210> 448 <211> 20 <212> DNA <213> Brassica rapa <400> 448 aagttgtccg gagttcacaa 20 <210> 449 <211> 20 <212> DNA <213> Brassica rapa <400> 449 tccaagatgg tgtgtgtgag 20 <210> 450 <211> 20 <212> DNA <213> Brassica rapa <400> 450 cgcaactatt gacgctcttt 20 <210> 451 <211> 20 <212> DNA <213> Brassica rapa <400> 451 gtggaggcaa aatttggtaa 20 <210> 452 <211> 20 <212> DNA <213> Brassica rapa <400> 452 gcaattgggt gttcagtttc 20 <210> 453 <211> 20 <212> DNA <213> Brassica rapa <400> 453 tcttctattg gcaatctggt 20 <210> 454 <211> 20 <212> DNA <213> Brassica rapa <400> 454 ttgttagggc tcgttgagac 20 <210> 455 <211> 22 <212> DNA <213> Brassica rapa <400> 455 ttcatacgag catctgcata ac 22 <210> 456 <211> 22 <212> DNA <213> Brassica rapa <400> 456 ttaaaccact tgaaccgaaa ct 22 <210> 457 <211> 20 <212> DNA <213> Brassica rapa <400> 457 ctaaggccaa gaaagggaag 20 <210> 458 <211> 20 <212> DNA <213> Brassica rapa <400> 458 tgcttatgcc cagataaagc 20 <210> 459 <211> 23 <212> DNA <213> Brassica rapa <400> 459 ccggtatgaa tattccattg cta 23 <210> 460 <211> 22 <212> DNA <213> Brassica rapa <400> 460 tgaaatcttc ctcccaaaga aa 22 <210> 461 <211> 23 <212> DNA <213> Brassica rapa <400> 461 ggttgacgtc tcattgtgtt ctt 23 <210> 462 <211> 23 <212> DNA <213> Brassica rapa <400> 462 tagctttgct cacttttcac tcc 23 <210> 463 <211> 23 <212> DNA <213> Brassica rapa <400> 463 acaattgttc taggcgttca aaa 23 <210> 464 <211> 23 <212> DNA <213> Brassica rapa <400> 464 atcagtatcc tggttctggt tca 23 <210> 465 <211> 20 <212> DNA <213> Brassica rapa <400> 465 gtatccttgg tgggcctagt 20 <210> 466 <211> 20 <212> DNA <213> Brassica rapa <400> 466 tgaccatctc aatgactcca 20 <210> 467 <211> 23 <212> DNA <213> Brassica rapa <400> 467 acacaaaggc aaaagagtag ctg 23 <210> 468 <211> 23 <212> DNA <213> Brassica rapa <400> 468 gaacctaatg aaatgcacaa agc 23 <210> 469 <211> 20 <212> DNA <213> Brassica rapa <400> 469 tcaccgccat aatttgatcc 20 <210> 470 <211> 21 <212> DNA <213> Brassica rapa <400> 470 ccctctcaac aaggtatgca a 21 <210> 471 <211> 20 <212> DNA <213> Brassica rapa <400> 471 gggtatttcc ggtttaggaa 20 <210> 472 <211> 20 <212> DNA <213> Brassica rapa <400> 472 ccctcttcct ctcaacacct 20 <210> 473 <211> 20 <212> DNA <213> Brassica rapa <400> 473 ttcccaagct tgctggtact 20 <210> 474 <211> 20 <212> DNA <213> Brassica rapa <400> 474 gagatttccc tcgcttgatg 20 <210> 475 <211> 20 <212> DNA <213> Brassica rapa <400> 475 cacgtcgggt taaattcttg 20 <210> 476 <211> 20 <212> DNA <213> Brassica rapa <400> 476 tgtttagggg ctcctcattt 20 <210> 477 <211> 20 <212> DNA <213> Brassica rapa <400> 477 cgggcagact gtttctgtat 20 <210> 478 <211> 20 <212> DNA <213> Brassica rapa <400> 478 ttcctccttt gaaccaatca 20 <210> 479 <211> 20 <212> DNA <213> Brassica rapa <400> 479 acttctccgg ctggatactc 20 <210> 480 <211> 20 <212> DNA <213> Brassica rapa <400> 480 ccgtttaaac ttgcgtttgt 20 <210> 481 <211> 23 <212> DNA <213> Brassica rapa <400> 481 cagtgtgatg ttgaaagaga atg 23 <210> 482 <211> 22 <212> DNA <213> Brassica rapa <400> 482 tcaaatctag agaatcccct ct 22 <210> 483 <211> 20 <212> DNA <213> Brassica rapa <400> 483 ccgtgaccgc ttcttacgat 20 <210> 484 <211> 20 <212> DNA <213> Brassica rapa <400> 484 caggcctttt aaaccgcaac 20 <210> 485 <211> 23 <212> DNA <213> Brassica rapa <400> 485 tgcctttata agagttagac cga 23 <210> 486 <211> 20 <212> DNA <213> Brassica rapa <400> 486 aacttttcca atgtgtgctt 20 <210> 487 <211> 19 <212> DNA <213> Brassica rapa <400> 487 ttcacacaag gtttgtgcc 19 <210> 488 <211> 20 <212> DNA <213> Brassica rapa <400> 488 cgtaaaggca tcaaggaaaa 20 <210> 489 <211> 19 <212> DNA <213> Brassica rapa <400> 489 gaatttctga cttggcgct 19 <210> 490 <211> 23 <212> DNA <213> Brassica rapa <400> 490 aaaacaaggt tgaatactac gca 23 <210> 491 <211> 18 <212> DNA <213> Brassica rapa <400> 491 ggacagccga catagctg 18 <210> 492 <211> 21 <212> DNA <213> Brassica rapa <400> 492 ctgtttatcg gaccattgtt g 21 <210> 493 <211> 20 <212> DNA <213> Brassica rapa <400> 493 ttagtggcga tcctgagaag 20 <210> 494 <211> 20 <212> DNA <213> Brassica rapa <400> 494 atcatcgaca tcatcagcct 20 <210> 495 <211> 22 <212> DNA <213> Brassica rapa <400> 495 gctggttcca gctaatggtt ac 22 <210> 496 <211> 23 <212> DNA <213> Brassica rapa <400> 496 tgaatttatg agattcggat tgg 23 <210> 497 <211> 23 <212> DNA <213> Brassica rapa <400> 497 cacaaataaa ccgttaaaca tca 23 <210> 498 <211> 20 <212> DNA <213> Brassica rapa <400> 498 gagctgaaaa ggatatgcca 20 <210> 499 <211> 21 <212> DNA <213> Brassica rapa <400> 499 aacagtttct acgcatcgtg t 21 <210> 500 <211> 22 <212> DNA <213> Brassica rapa <400> 500 gcgttttcga ttttaaattt tg 22 <210> 501 <211> 20 <212> DNA <213> Brassica rapa <400> 501 tgtcgccatt ttactgttcg 20 <210> 502 <211> 20 <212> DNA <213> Brassica rapa <400> 502 attgcaatct tcctccaacg 20 <210> 503 <211> 20 <212> DNA <213> Brassica rapa <400> 503 gtggattttt gcattcttgg 20 <210> 504 <211> 20 <212> DNA <213> Brassica rapa <400> 504 gaaaaaggaa aaattgggga 20 <210> 505 <211> 22 <212> DNA <213> Brassica rapa <400> 505 gagaagaatt ttcccactat gc 22 <210> 506 <211> 27 <212> DNA <213> Brassica rapa <400> 506 gattttattg acaatgtata tttgctg 27 <210> 507 <211> 20 <212> DNA <213> Brassica rapa <400> 507 atcagtgccg gccttgaata 20 <210> 508 <211> 21 <212> DNA <213> Brassica rapa <400> 508 aagcttgagc ttccagcctt c 21 <210> 509 <211> 23 <212> DNA <213> Brassica rapa <400> 509 ttgaataatg accccaaata tca 23 <210> 510 <211> 26 <212> DNA <213> Brassica rapa <400> 510 tcaataggta ttaaccaatt ctaccg 26 <210> 511 <211> 21 <212> DNA <213> Brassica rapa <400> 511 agggagactg gtagagagga a 21 <210> 512 <211> 20 <212> DNA <213> Brassica rapa <400> 512 cgttcaggat ttttagggct 20  

Claims (2)

배추 유전체 염기서열로부터 개발된 서열번호 1 내지 512의 미세 부수체 분자표지의 프라이머 쌍.Primer pair of microacoustic molecular labels of SEQ ID NOs: 1 to 512 developed from Chinese cabbage genome sequences. 제1항의 미세 부수체 분자표지를 이용하여 장원 F3-풀드 집단에 대해 작성된 도 4의 유전자지도.The genetic map of FIG. 4 prepared for the manor F3-pulled population using the microacoustic molecular label of claim 1.
KR1020080013786A 2008-02-15 2008-02-15 Development of sequence-based microsatellite markers in brassica rapa and construction of genetic map KR20090088492A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101111563B1 (en) * 2009-09-24 2012-06-18 충남대학교산학협력단 Chinese cabbage BAC clone-derived SSR markers and construction of reference genetic map in Brassica rapa
CN102703445A (en) * 2012-05-31 2012-10-03 山东省农业科学院蔬菜研究所 Specific molecular markers of eIF4E.a mutation site of Chinese cabbage and application of Specific molecular markers
CN110512022A (en) * 2019-09-02 2019-11-29 中国农业科学院蔬菜花卉研究所 Identify the molecular labeling and method of Vegetables in Brassica interspecific hybrid and progeny material A06 and C05 chromosome separation situation
CN112442549A (en) * 2020-12-21 2021-03-05 浙江大学 Polymorphic SSR primer and method for constructing turnip primary core germplasm
CN113862392A (en) * 2021-11-15 2021-12-31 西北农林科技大学 SSR molecular marker primer linked with Chinese cabbage yellow cotyledon gene Bryc and application thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101111563B1 (en) * 2009-09-24 2012-06-18 충남대학교산학협력단 Chinese cabbage BAC clone-derived SSR markers and construction of reference genetic map in Brassica rapa
CN102703445A (en) * 2012-05-31 2012-10-03 山东省农业科学院蔬菜研究所 Specific molecular markers of eIF4E.a mutation site of Chinese cabbage and application of Specific molecular markers
CN102703445B (en) * 2012-05-31 2013-07-24 山东省农业科学院蔬菜研究所 Specific molecular markers of eIF4E.a mutation site of Chinese cabbage and application of Specific molecular markers
CN110512022A (en) * 2019-09-02 2019-11-29 中国农业科学院蔬菜花卉研究所 Identify the molecular labeling and method of Vegetables in Brassica interspecific hybrid and progeny material A06 and C05 chromosome separation situation
CN112442549A (en) * 2020-12-21 2021-03-05 浙江大学 Polymorphic SSR primer and method for constructing turnip primary core germplasm
CN113862392A (en) * 2021-11-15 2021-12-31 西北农林科技大学 SSR molecular marker primer linked with Chinese cabbage yellow cotyledon gene Bryc and application thereof

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