KR101432279B1 - SSR markers and Genetic linkage map using Intraspecific population of Capsicum annuum - Google Patents

Abstract

The present invention provides a DNA marker for mapping a capsicum anium gene, and a primer set capable of amplifying the DNA marker, and a primer set capable of amplifying the DNA marker, developed using an intraspecies population of Capsicum annuum plants. The set is used for the creation of a high-density genetic association map of Capsicum annium, which can be very useful for molecular breeding.

Description

SSR markers and Genetic linkage map using Intraspecific population of Capsicum annuum}

The present invention is an SSR marker for generating a capsicum annium gene association map developed using an intraspecific population of a capsicum annuum plant, and a gene association map of a capsicum annium prepared by performing the SSR analysis. It is about.

Red pepper is a crop belonging to the family Solanacea, and grows as a perennial in the tropics and as a perennial in the temperate regions. As the main agricultural product after rice in Korea, the per capita consumption of pepper is the highest in the world (Ministry of Agriculture and Forestry). Peppers used as cultivars are five species, including C. annuum , C. baccatum , C. chinense , C. frutescens , and C. pubescens , including wild species, and more than 25 species have been reported to date. After spreading to Africa, it has been widely cultivated in tropical regions including Africa and South America, subtropical regions such as Indonesia and Thailand, and temperate monsoon regions such as Korea and China (Andrews, 1990).

Recently, pepper breeders are maximizing the efficiency of traditional breeding methods by performing both traditional and molecular breeding methods. The useful traits of red pepper are divided into qualitative traits dominated by one dominant or recessive gene and quantitative traits dominated by several genes. Studies have shown that the quality of red pepper is spicy, orange and red of the fruit, resistance to bacterial spot disease, Potato virus Y (PVY) and tobacco mosaic virus (TMV) resistance, and male infertility. The traits are known for the shape or weight of the family, resistance to late blight and anthrax, and resistance to the Cucumber mosaic virus (CMV) (Boukema, 1980; Carnata et al ., 1996; John et al ., 1988; Carnata et al ., 2002; Huh et al ., 2001; Lefebvre et al ., 1995; Tai et al ., 1999).

Qualitative traits are clearly separated from posterity according to Mendel's Independent Genetic Law, so they can be used for breeding relatively easily compared to quantitative traits. However, in the case of quantitative traits, it is difficult to cultivate varieties with target traits easily because of the environmental factors involved in the interaction and expression of genes such as additive effect or epistatic effect. In this regard, if the breeder has a marker for a useful gene, the breeder will be able to efficiently cultivate excellent varieties, which will be made possible by utilizing molecular labels.

The initial molecular labeling method was very difficult to analyze, but currently, most of the label development using PCR method that can be easily used for breeding has increased the weight of the molecular label in pepper breeding.

According to these demands, the present inventors developed SSR markers and capsicum annuum high-density gene association maps using interbreeding of capsicum annuum species, particularly suitable for use in pepper breeding, The present invention was completed by verification through comparison with conventional SNU3 and TMDH maps.

An object of the present invention is to provide a capsicum anium gene-related mapping SSR marker and primers for amplification thereof, and their use, developed from various sequencing information of a capsicum annuum plant that is a cultivar of red pepper. .

Another object of the present invention is to provide a genetic association map of capsicum anium using the population of capsicum anium species and the SSR marker.

Another object of the present invention is to provide a comparative map of the SNU3 and TMDH maps with the genetically related maps of the capsicum annium of the present invention.

In order to solve the above problems, the present invention provides a SSR (Sinple Sequence Repeat) marker for gene association mapping of Capsicum annuum .

In particular, the SSR marker is described in Table 3, and having a locus on a gene-related map of Capsicum annuum of FIGS. 1A to 3D, 79 markers derived from BAC clones shown in FIGS. 1A to 3D, It is preferable to include 187 markers derived from the NCBI sequence data base, 140 markers derived from the cDNA library, and 9 markers associated with traits.
More specifically, the present invention amplifies genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. Average between the adjacent loci of F2 population Capsicum anium plants obtained by self-pollination of F1 produced by crossing between the capsicum annuum CM334 and Capsicum annuum ECW123R, which is an intraspecies group selected from the generated DNAs. High-density gene-related mapping DNA with a distance of 5.45 cM and a total length of 3,001 cM (where n is one of the integers from 1 to 549, 5, 6, 19, 26, 39, 40, 49, 50, 61 , 71, 121, 124, 135, 151, 161, 172, 175, 220, 222, 241, 252, 262, 268, 277, 279, 293, 304, 306, 315, 320, 329, 353, 358, 383 , 391, 404, 405, 436, 444, 447, 511, 534 and 539 are excluded).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. The gene association group 1 of the F2 population capsicum anium plant obtained by self-pollination of F1 produced by a cross between a capsicum annuum CM334 and a capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs Marker DNA for high-density gene association mapping with an average distance between adjacent loci of 5.45 cM (where n is one of the integers from 1 to 81, 5, 6, 19, 26, 39, 40, 49, 50, 61 And 71 are excluded).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by auto-pollination of a cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, which is an intraspecies group selected from among the DNAs of the DNA group Provides high-density gene association mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of the integers of 82 to 140, excluding 121, 124, and 135).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of the cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, which is a species selected from among the DNAs of the gene group of the gene association group 3 of the capsicum anium plant Provides high-density gene-related mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of 141 to 218 integers, 151, 161, 172, and 175 are excluded).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of the cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, an intraspecies group selected from among DNAs of the gene group of the gene association group 4 of the capsicum anium plant Provides high-density gene association mapping marker DNA having an average distance between adjacent loci of 5.45 cM (where n is one of 219 to 237 integers, excluding 220 and 222).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of a cross between a capsicum annuum CM334 and a capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs, are genetically related to the group 5 of the capsicum annium plant Provides high-density gene association mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of 238 to 269 integers, excluding 241, 252, 262, and 268).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by auto-pollination of a cross between a capsicum annuum CM334 and a capsicum annuum ECW123R, which is an intraspecies group selected from among the DNAs of the gene, of the gene association group 6 of the capsicum anium plant Provides high-density gene association mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of 270 to 305 integers, excluding 277, 279, 293, and 304).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of a cross between a capsicum annuum CM334 and a capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs, of the genetic association group 7 of the capsicum anium plant Provides high-density gene association mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of the integers from 306 to 345, 306, 315, 320, and 329 are excluded).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by auto-pollination of a cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs, of the genetic association group 8 of the capsicum anium plant Provides high-density gene association mapping DNA with an average distance between adjacent loci of 5.45 cM (where n is one of 346 to 392 integers, excluding 353, 358, 383, and 391).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by auto-pollination of a cross between a capsicum annuum CM334 and a capsicum annuum ECW123R, which is a species selected from among the DNAs of the gene group of gene association group 9 of the capsicum anium plant Provides high-density gene association mapping marker DNA having an average distance between adjacent loci of 5.45 cM (where n is one of 393 to 444 integers, 404, 405, 436, and 444 are excluded).
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination between a cross between capsicum annuum CM334 and capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs, are genetically related to group 10 of capsicum anium plants A marker DNA for mapping a high-density gene having an average distance between adjacent loci of 5.45 cM (where n is one of 445 to 471, 447 is excluded) is provided.
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of the cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, which is an intraspecies group selected from among DNAs, of the gene association group 11 of the capsicum anium plant A marker DNA for mapping a high-density gene with an average distance between adjacent loci of 5.45 cM (where n is one of the integers of 472 to 509) is provided.
The present invention is further generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1. F2 group obtained by self-pollination of the cross between the capsicum annuum CM334 and the capsicum annuum ECW123R, which is an intraspecies group selected from among the DNAs of the DNA group Provides high-density gene association mapping marker DNA having an average distance between adjacent loci of 5.45 cM (where n is one of 510 to 549 integers, 511, 534, and 539 are excluded).

The gene association map can be used as a population for gene association mapping using the F2 group obtained by self-pollinating F1 generated by a cross between the species group Capsicum annuum CM334 and Capsicum annuum ECW123R. .

In addition, the present invention provides a primer set for amplifying the SSR markers, including 43 SSR primers derived from SNU3 and TMDH maps and 93 web search SSR primers for genetic mapping of the capsicum annuum . do. Preferably it is the primer set described in Table 3.
More specifically, the present invention is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsi, an intraspecies population. Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) crossing the F1 self-pollination a F2 population kaepsi Com annyum locus average distance of 5.45 cM between adjacent plants obtained by generation in between ECW123R and General 3,001 cM of high density gene Primer set for amplification of marker DNA for association mapping (where n is one of the integers from 1 to 549, 5, 6, 19, 26, 39, 40, 49, 50, 61, 71, 121, 124 , 135, 151, 161, 172, 175, 220, 222, 241, 252, 262, 268, 277, 279, 293, 304, 306, 315, 320, 329, 353, 358, 383, 391, 404, 405 , 436, 444, 447, 511, 534 and 539 are excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene linkage group 1 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by a cross between an annium ( Capsicum annuum ) CM334 and a Capsicum annuum ECW123R Primer set for amplification of marker DNA for association mapping (where n is one of 1 to 81, 5, 6, 19, 26, 39, 40, 49, 50, 61 and 71 are excluded) Provides
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High density gene with an average distance between adjacent loci of gene linkage group 2 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of the integers of 82 to 140, and 121, 124, and 135 are excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene linkage group 3 of the F2 population Capsicum anium plant obtained by auto-pollination of F1 generated by cross-hybridization between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of 141 to 218, 151, 161, 172, and 175 are excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene association group 4 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by a cross between an annium ( Capsicum annuum ) CM334 and a Capsicum annuum ECW123R. A primer set for amplification of the marker DNA for mapping is provided (where n is one of 219 to 237, 220 and 222 are excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High density gene with an average distance between adjacent loci of gene linkage group 5 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of 238 to 269, excluding 241, 252, 262, and 268).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene linkage group 6 of the F2 population Capsicum anium plant obtained by auto-pollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of the integers from 270 to 305, excluding 277, 279, 293, and 304).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene association group 7 of the F2 population Capsicum anium plant obtained by auto-pollination of F1 generated by cross-hybridization between Ansium ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of 306 to 345, 306, 315, 320, and 329 are excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of gene linkage group 8 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of marker DNA for association mapping (where n is one of 346 to 392, 353, 358, 383, and 391 are excluded) is provided.
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High density gene with an average distance between adjacent loci of gene linkage group 9 of the F2 population Capsicum anium plant obtained by self-pollination of F1 generated by a cross between an anium ( Capsicum annuum ) CM334 and a Capsicum annuum ECW123R Provides a primer set for amplification of the marker DNA for mapping, wherein n is one of 393 to 444, 404, 405, 436 and 444 are excluded.
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High density gene with a mean distance between adjacent loci of gene linkage group 10 of F2 population Capsicum anium plants obtained by autopollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of the integers from 445 to 471, and 447 is excluded).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of the gene-related group 11 of the F2 population Capsicum anium plant obtained by auto-pollination of F1 generated by a cross between an annium ( Capsicum annuum ) CM334 and a Capsicum annuum ECW123R A primer set for amplification of the marker DNA for mapping is provided (where n is one of the integers of 472 to 509).
The present invention furthermore, is selected from among primer sets comprising a forward primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of a nucleic acid sequence represented by SEQ ID NO: 2n, a capsicum, an intraspecies population. High-density gene with an average distance between adjacent loci of the gene-related group 12 of the F2 population Capsicum anium plant obtained by auto-pollination of F1 generated by crossing between Ansi ( Capsicum annuum ) CM334 and Capsicum annuum ECW123R Provides a primer set for amplification of marker DNA for association mapping (where n is one of 510 to 549, 511, 534, and 539 are excluded).

The primer set for amplifying the SSR marker is preferably 100 to 300 bp in size, 57 to 63°C in Tm, and 20 to 80% in GC ratio.

In addition, the present invention is the pepper (Capsicum) registered in the database, the method comprising selecting a BAC clone repeating the SSR motif 1 ~ 6 bp from the nucleotide sequence information of the BAC clone; And designing and constructing a set of SSR primers for mapping 1 to 3 genes per BAC clone of claim 3 from the flanking base sequence of the SSR motif of the selected BAC clone. A method for selecting a set of SSR primers for production is provided.

In particular, in this case, it is preferable that the number of SSR repeat motifs is repeated 10 times or more for 1 bp base and 5 or more times for 2 to 6 bp base.

In addition, the present invention provides a high-density gene-related map of capsicum anium of FIGS. 1A to 3D, which is prepared by performing SSR analysis on capsicum annuum as a group for generating gene-related maps.

In one embodiment, the embodiment of the present invention was prepared using a F2 population obtained by self-pollination of F1 generated by a cross between a species population of Capsicum annuum CM334 and Capsicum annuum ECW123R. . At this time, the F2 population was obtained and analyzed.

In particular, the high-density gene association map of capsicum annium of the present invention has an average distance between adjacent loci of 5.45 cM, and is characterized by a total length of 3,001 cM, and 43 SSR primers derived from SNU3 and TMDH maps assigned to association groups 1-12, Includes 79 markers from BAC clone, 187 markers from NCBI sequence database, 140 markers from cDNA library, 93 web search SSR primers, and 9 markers associated with traits.

delete

As such, the present invention is a capsicum anium ( Capsicum) annuum ) X Provides SSR markers developed from various sequencing information for Capsicum annuum and genetic association maps of peppers prepared using them and uses thereof.

The SSR marker for gene association mapping and gene association map of the present invention can be utilized in maker assisted selection (MAS) to shorten the breeding age of Capsicum annuum and contribute to the search for useful genes and traits, It can be used as a basic data for the study of species differentiation and genome duplication of the branch family. In particular, the present invention is more advantageous for breeding because it uses an interspecific population of capsicum anium.

Figure 1a is a capsicum annuum ( Capsicum annuum ) CM334 and Capsicum annuum ( Capsicum annuum ) ECW123R generated by cross-pollination of F1 obtained by self-pollination of the present invention was created using a population of F2 capsicum annium gene association group 1 The high-density gene association map is FnP(AA)_1.
Figure 1b is kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) of the second gene associated group of kaepsi Com annyum of the present invention prepared by using a F2 population obtained by the water self-cost F1 generated by mating between ECW123R The high-density gene association map is FnP(AA)_2.
In Figure 1c kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) gene of kaepsi Com annyum of the present invention created by the water self the F1 generation by mating using the F2 population obtained between ECW123R linkage group 3 The high-density gene association map is FnP(AA)_3.
Of Figure 2a kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) gene associated with the group of kaepsi Com annyum of the present invention prepared by using a F2 population obtained by the water self-cost F1 generated by mating between ECW123R 4 The high-density gene association map is FnP(AA)_4.
Figure 2b is a capsicum annuum ( Capsicum annuum ) CM334 and capsicum annuum ( Capsicum annuum) ECW123R generated by cross-pollination of F1 obtained by self-pollination of the present invention was created using a population of F2 capsicum annium gene association group 5 The high-density gene association map is FnP(AA)_5.
Figure 2c is a capsicum annuum ( Capsicum annuum ) CM334 and capsicum annuum ( Capsicum annuum) ECW123R generated by cross-pollination of F1 obtained by self-pollination of the present invention was created using a population of F2 capsicum annium gene association group 6 The high-density gene association map is FnP(AA)_6.
Of Figure 2d is kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) gene of kaepsi Com annyum of the present invention self-cost F1 generated by mating created using the F2 population obtained by moisture between ECW123R associated group 7 The high-density gene association map is FnP(AA)_7.
Figure 2e is kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) Self-cost F1 generated by mating moisture of a gene associated with the group of F2 kaepsi Com annyum of the present invention prepared by using a group of 8 obtained between ECW123R The high-density gene association map is FnP(AA)_8.
Figure 3a is a capsicum annuum ( Capsicum annuum ) CM334 and Capsicum annuum ( Capsicum annuum) ECW123R generated by cross-pollination of F1 obtained by self-pollination of the present invention created using the population of F2 capsicum annu gene association group 9 The high-density gene association map is FnP(AA)_9.
Figure 3b is a capsicum annuum ( Capsicum annuum ) CM334 and Capsicum annuum ( Capsicum annuum) ECW123R generated by cross-pollination of the F1 obtained by self-pollination of the population of the present invention, the capsicum annium gene association group 10 of The high-density gene association map is FnP(AA)_10.
Figure 3c is a capsicum annuum ( Capsicum annuum ) CM334 and Capsicum annuum ( Capsicum annuum) ECW123R generated by cross-pollination of F1 obtained by self-pollination of the present invention was created using the population of F2 capsicum annium gene association group 11 The high-density gene association map is FnP(AA)_11.
Of Figure 3d kaepsi Com annyum (Capsicum annuum) CM334 and kaepsi Com annyum (Capsicum annuum) crossing the F1 self-pollination by the genes of kaepsi Com annyum of the present invention prepared by using a F2 population obtained linkage groups generated in between ECW123R 12 The high-density gene association map is FnP(AA)_12.

Definitions of terms used in the present invention are as follows.

'Polymorphism' refers to a case in which a gene mutation occurs at a frequency of at least 1% or more in a population. Protein polymorphism, the variation of one nucleotide to the protein product of alleles is called single nucleotide polymorphism (SNP).

'Marker' refers to the nucleotide sequence used as a reference point when identifying a locus or associated locus or a coding product (eg, protein) thereof. Markers can be derived from genomic nucleotide sequences or from expressed nucleotide sequences (eg, from RNA, nRNA, mRNA, cDNA, etc.), or from encoded polypeptides. The term includes nucleic acid sequences complementary to or flanking a marker sequence, such as a nucleic acid used as a probe or primer set capable of amplifying a marker sequence.

In particular, the present invention refers to a nucleotide sequence used as a reference point when identifying genetically unspecified associated loci. The position of a molecular marker on a gene-related map is called a genetic locus.

'Nucleotide' refers to a nucleic acid such as deoxyribonucleic acid (DNA), and, if appropriate, ribonucleic acid (RNA). The term also equally includes analogs of either RNA or DNA prepared from nucleotide analogs (eg, peptide nucleic acids), and single (sense or antisense) and double helix polynucleotides.

The'linkage map' is a genetic map showing the relative position of a gene, and shows their relative chromosomal position according to the genetic property pattern of DNA markers (different DNA sequence/SNP, SSR different from the gene). The distance between markers on the map indicates how often they are inherited together. Genetic association maps are constructed by observing how frequently two markers are inherited together in a family tree. The association map is completed through a mapping operation in which a group showing random polymorphism between parents is converted into a recombination value of each other in a randomly nurtured experimental group, and their order is determined to establish a linkage group between markers ( Jones et al ., 1997)

'Marker-assisted selection (MAS)' is a method that can be applied to crop breeding with molecular markers that are co-located or closely related to loci that dominate the target trait. In the case of using MAS, it is possible to shorten the time required to breed a new cultivar because the plant with the target trait is not grown until expression or the phenotype is tested, and the genotype of the target trait can be determined initially (Asins, 2002). ).

A'comparative map' is a map of a conserved chromosomal segment obtained by comparing the chromosomal location of homologous genes in different species.

Hereinafter, the present invention will be described in detail.

The present invention relates to a genetic association map of a red pepper belonging to a branch (Solanacea), that is, a SSR marker developed from various sequencing information derived from Capsicum annuum , a species of a plant of the genus Capsicum , and a pepper created using the same It is about.

The present invention relates to a high-density gene association map of these peppers, especially Capsicum annuum . They are used in MAS and MAB to find pepper breeding and useful genes and traits.

Gene-related maps can not only greatly contribute to the improvement of breeding efficiency by MAS, but are also used as a useful material for comparative genetics of other genes using cloning and co-labeling based on molecular maps (Grube et al ., 2000; Pierre et al ., 2000; Prince et al ., 1993). Recently, it is used for background selection of marker-assisted breeding (MAB). It is effectively used to remove chromosomes from donors after mating. The more dense a gene-related map is, the more individuals can be selected with minimal donor chromosomes.

The genome of pepper is estimated to be 2,702 to 3,420 Mbp in size (Arumuganathan and Earle, 1991), and the genetic association map of pepper is isozyme marker (Tanksley, 1984) and tomato-derived marker from C. annuum X C. chinense combination ( Prince et al ., 1993; Tanksley et al ., 1988), and since then, it is a genetic association map that contains various types of markers such as RFLP, AFLP, RAPD, and SSR markers, which are produced by using pepper as a material. Is being made. In Korea, SNU2 (Lee et a l., 2004), which has 46 SSR and 287 RFLP labels, and 180 SSR and 63 RFLP labels, SNU3 (Yi et al ., 2006), are integrated. An associated map was prepared.

The high-density gene association map of red pepper plays a decisive role in increasing the accuracy of quantitative trait loci (QTL) analysis, thereby increasing the opportunity to perform useful gene separation and related marker searches in large quantities (Djian-Caporalino et al. ., 2001). In addition, the more accurately the location of QTL with respect to the target trait or the more molecular markers present in the same or adjacent position to QTL, the efficiency of red pepper breeding through MAS is improved. The limited number of available markers, if limited only in the developed population, is directly related to the improvement of breeding efficiency by MAS. Marker-assisted selection (MAS) is a method that can be applied to crop breeding with molecular markers that are co-located or closely related to the locus that controls the target trait.

Therefore, the present invention can also be usefully used for this purpose.

In one aspect, the present invention relates to a SSR (Sinple Sequence Repeat) marker for genetic mapping of Capsicum annuum plants.

“Marker” refers to the sequence used as a reference point when identifying genetically unspecified associated loci. The term also applies to nucleic acid sequences complementary to a marker sequence, such as a nucleic acid used as a primer pair capable of amplifying a marker sequence. The location of a molecular marker on a genetic association map is called a genetic locus.

Preferably, the genetic linkage map SSR marker for creating pepper BAC clones derived from the 79 markers with genomic DNA BAC-end sequencing as described in the Fig. 1a to display the associated group right of Figure 3d with the Capsicum) ( Blue), 187 markers from the NCBI sequence data base (black), 140 markers from the cDNA library (red), and 9 markers associated with the trait (pink).

In the present invention, the SSR (Sinple Sequence Repeat) marker is for gene mapping of plants of the genus Capsicum annuum .

In particular, as a group for mapping genetic associations, genetic association maps of peppers created using SSR developed from various sequencing information for intra-species populations, namely, Capsicum annuum x Capsicum annuum To write. In one embodiment, the genetic map is created for the group F2 population obtained by self pollination of the F1 generation to the mating between kaepsi Com annyum (Capsicum annuum) and CM334 kaepsi Com annyum (Capsicum annuum) ECW123R.

In addition, the present invention includes primers for amplifying SSR markers for genetic mapping of capsicum anium plants.

"Primer" refers to a single-stranded oligonucleotide sequence complementary to the nucleic acid strand to be copied, and may serve as a starting point for the synthesis of primer extension products. The length and sequence of the primer should allow the synthesis of the extension product to begin. The specific length and sequence of the primer will depend on the desired DNA or RNA target complexity, as well as the conditions of primer use, such as temperature and ionic strength. As used herein, oligonucleotides used as primers may also include nucleotide analogs, such as phosphorothioate, alkylphosphorothioate or peptide nucleic acid, or May include intercalating agents

Primer used in the present invention, the length of the amplified DNA is 100-300 bp, the melting point (melting temperature, Tm) value between the forward and reverse primers is 3 ℃ or less and the overall Tm value is within 57 ℃ to 63 ℃ It is preferred. In addition, the GC content is preferably 20% to 80%. As a preferred example, there are SNU3, 43 SSR primers derived from TMDH map, 93 web search SSR primers (brown), and the like shown in Table 3.

On the other hand, SSR markers and primers used in the present invention can be found by the following method.

First, for example, SSR markers are found through BAC-end sequencing. In one example, FP11E and FP11H BAC library sequenced 18,432 clones to obtain 15,609 BAC-end sequences, from which primers containing 5,106 SSRs were primer3 software (http://frodo.wi.mit.edu It can be synthesized using /primer3/) and used for polymorphism analysis. And, more than 120,000 Capsicum nucleotide sequences can be secured using NCBI site (http://www.ncbi.nlm.nih.gov/) and used for SSR primer synthesis.

The present invention also comprises the steps of isolating genomic DNA from pepper; Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set according to the present invention; And detecting the amplification product, identifying a SSR marker for mapping a pepper gene association.

The method of the present invention includes isolating genomic DNA from Chinese cabbage. The method of separating genomic DNA from the sample may use methods known in the art, for example, a CTAB method, or a wizard prep kit. Using the isolated genomic DNA as a template, an amplification reaction can be performed using the primer to amplify the target sequence. Methods for amplifying target nucleic acids include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification system, There are any other suitable methods for amplification via strand displacement amplification or Qβ replication enzymes or amplification of nucleic acid molecules known in the art. Among them, PCR is a method of amplifying a target nucleic acid from a primer set that specifically binds to the target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used.

Therefore, the present invention is the primer set; And a kit for identifying SSR markers for capsicum annium gene association mapping, including reagents for performing an amplification reaction.

In the kit of the present invention, the primer set is as described above, and the reagent for performing the amplification reaction may include heat-resistant DNA polymerase, dNTPs, buffer, and the like. In addition, the kit of the present invention may further include a user guide describing optimal conditions for performing the reaction. The handbook is a printout that explains how to use the kit, for example, how to make PCR buffers, and the reaction conditions presented. The guide includes brochures in the form of brochures or flyers, labels attached to the kit, and descriptions on the surface of the package containing the kit. In addition, the handbook includes information that is disclosed or provided through electronic media, such as the Internet.

The present invention also includes selecting a BAC clone comprising an SSR motif of ≥18 bp from the sequence information of the pepper BAC clone registered on the database; And designing and producing a set of SSR primers for mapping 1 to 3 genes per BAC clone from the flanking base sequence of the SSR motif of the selected BAC clone. A method for selecting a set of SSR primers is provided.

In particular, in the present invention, the SSR repeat motif is preferably from one base repeat (mono-) to six base repeats (hexa-), and the number of repeat motifs is one base repeat. It is preferable to make it more than 5 times that 10 or more times and 2 to 6 bases are repeated.

The present invention is a genetic linkage map of kaepsi Com annyum of Figures 1a - created by carrying out (Simple Sequence Repeat) SSR analysis on the kaepsi Com annyum (Capsicum annuum) in a group for mapping genetic linkage in another aspect 3d It is about.

In the gene-related map according to an embodiment of the present invention, the group for generating the gene-related map is a group generated by crossing a species group, Capsicum annuum x Capsicum annuum . In one embodiment, the genetic map is created for the group F2 population obtained by self pollination of the F1 generation to the mating between kaepsi Com annyum (Capsicum annuum) and CM334 kaepsi Com annyum (Capsicum annuum) ECW123R. The CM334 is resistant to the most damaging plague ( Phytophthora capsici ) of pepper , and ECW123R is a species that exhibits morbidity and is a very easy combination for the QTL analysis of late blight. "Resistant" may mean that the plant exhibits substantially no phenotypic changes as a result of substance administration, pathogen infection, or exposure to stress, and preferably means substantially no phenotypic changes upon pathogen infection. .

In addition, the gene-related map is characterized in that the average distance between adjacent loci is 5.45 cM and the total length is 3,001 cM.

In addition, the gene-related maps are SNU3 assigned to 12 related groups 1 to 12, 43 SSR primers derived from TMDH map, 79 markers derived from BAC clone, 187 markers derived from NCBI sequence data base, 140 markers derived from cDNA library, Includes 93 web search SSR primers and 9 markers associated with traits.

As described above, the high-density gene association map of the capsicum annuum of the present invention can be utilized in MAS and MAB to shorten the breeding time of red pepper and contribute to the search for useful genes and traits, as well as the speciation and genome of branch and plant It can be used as basic data for duplicate research.

delete

delete

Moreover, the genetic association maps of the existing peppers were obtained by crossing different species, so a molecular marker with high polymorphism was obtained. Have Therefore, the high-density gene association map of the capsicum annuum of the present invention is more suitable for use in breeding.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in practice to test the present invention, preferred materials and methods are described herein.

Example  1: Genome DNA  extraction

To extract gDNA of each individual from 12 different strains of pepper and F2 population, the following was performed.

First, 50 mg of leaves of each Capsicum annuum individual was CTAB buffer [2% (w/v) hexadecyltrimethylammonium bromide, 1.4 M sodium chloride, 30 mM EDTA, 100 mM Tris-HCl pH 8.0, 1% ( w/v) PVP, 0.2% (v/v) mercaptoethanol] 750 µl, each crushed, and incubated at 65°C for 1 hour.

Next, 750 µl of a chloroform:isoamyl alcohol (24:1) solution was added, mixed well, and centrifuged at 13,000 rpm for 10 minutes to obtain 500 µl of the supernatant. The DNA was precipitated by adding 1/10 the amount of sodium acetate and 2 times the amount of 100% ethanol. Next, after washing and drying with 70% ethanol, DNA was isolated by dissolving in RNase-added TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA).

Example  2 : SSR Marker  Development

(One) SSR Marker  Development

First, SSR was found through BAC-end sequencing, and the FP11E and FP11H　BAC libraries were used to sequence the SSR to sequence 18,432 clones to obtain 15,609 BAC-end sequences. From this, primers containing 5,106 SSRs were synthesized using Primer3 software (http://frodo.wi.mit.edu/primer3/) and used for polymorphism analysis. The contents of the BAC-end sequencing data are shown in Table 1 (sequence statistics) and Table 2 (sequence similarity search results).

Total clones 18,432 Total number of sequences 15,609 Total number of bases (base count, bp) 10,380 kb Average length (bp) 665 Genome coverage 0.37X

Database Number of hits (%) Mitochondria + Chloroplast 35 (0.2%) Transposon + Retrotransposon 928 (5.9%) NCBI protein database Protein 14,102 (90.3%) Unknown 544 (3.5%) Total number of BAC ends 15,609 (100%)

Then, more than 120,000 Capsicum nucleotide sequences were obtained using the NCBI site (http://www.ncbi.nlm.nih.gov/) and used for SSR primer synthesis.

As a condition used in the primer design, the length of the amplified DNA should be 100-300 bp, the melting temperature (Tm) value between the forward and reverse primers was 3°C or less, and the overall Tm value was within 57°C to 63°C. In addition, GC contents were set to 20% to 80% so that as many primers as possible were synthesized.

The SSR repeat motif was limited from repeating 1 base (mono-) to repeating 6 bases (hexa-), and the number of repeating motifs was 10 or more, and 2 repeating 1 base The repeat of 6 bases in was made 5 or more times. These SSR motif search and primer design are SSR finder 1.2b (http://jicbio.bbsrc.ac.uk/cereals/webstart/ssrfinder/ssrfinderlaunch.html) and SSRLocator l (http://minerva.ufpel.edu.br /~lmaia.faem/ssr_install_guide.html, Luciano et al ., 2008) was used. The primers thus synthesized were first subjected to polymorphism verification for 12 representative strains.

The primers used are listed in Table 3 below.

In particular, primers having the nucleic acid sequences of SEQ ID NOs: 1 to 1098 include primers represented by FnP groups produced by the present inventors, and those included in known SNU3 and TMDH maps, but these are high-density genetic associations of the present invention. It was included in the map and has its own new location.

(2) DNA amplification and PAGE analysis using SSR primers

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

delete

PCR amplification has a final volume of 25 μl, 30-50 ng genomic DNA template, 10 pmole SSR primer, 0.5 unit Taq polymerase (Solgent, Korea), 10X PCR buffer and sterile distilled water were used.

The amplification was performed in a general PCR machine, and the conditions were 94°C 4 minutes in the denaturation step, 94°C 45 seconds in the amplification step, 55°C 45 seconds, and 72°C 45 seconds, and 35 cycles were repeated. Finally, the reaction was performed at 72°C for 10 minutes, and all reactions were terminated at 8°C.

In general, SSR is amplified DNA fragments are less than 100-300 bp in length, and polymorphisms are small, ranging from 1 to several tens of bp, so polymorphism cannot be found in general agarose gel analysis. 1989) electrophoresis and DNA staining was observed using a rapid silver staining method (Sanguinetti et al., 1994).

Example  3: Genetic mapping

The combination of red pepper used in the present invention was Capsicum annuum CM334 X Capsicum annuum ECW123R. Among them, CM334 was resistant to the most damaging plague ( Phytophthora capsici ) of pepper , and ECW123R was a species that showed morbidity, and a very easy combination was selected for QTL analysis of late blight.

In particular, most of the pepper gene related maps use a population with favorable polymorphism using an interspecific population of Capsicum, whereas in the present invention, a C. annuum intraspecific population corresponding to a hybrid used by most breeders is used. To create a gene-related map.

All of these mated F1s were heterotypes, and 190 individuals from the F2 group obtained by self-pollination were used for genetic mapping. The obtained F2 group appears as a 1:2:1 genotype according to Mendel's Independent Genetic Law, and the molecular markers are collected to calculate each recombination value to create a genetic association map.

Mapdisto 1.7b (http://mapdisto.free.fr) was used for the mapping program for gene related maps. First, find groups are executed first, and LOD value is 6 and r max/d max value is 9~15 to group them. And followed the Kosambi mapping function (Kosambi, 1944).

The population parameter type was'F2' for the population type and '2' for the data format. As the recombination frequency, centimorgans (cM) was used as the unit for calculating the distance of the gene related map, and this also followed the Kosambi mapping function (Kosambi, 1943). The gene-related maps drawn in Mapdisto 1.7b were moved back to the MapChart program (Voorrips, 2002) and plotted, and comparative maps with other gene-related maps were prepared. In addition to the SSR molecular markers, screening was performed using previously known primers related to traits, and these were also listed in the gene association map (Table 4).

Marker name protocol Restriction enzymes Reference Size (bp) Group PH5-SCAR CAPS Dra I Pungency 1200 2 FPtr055 (SCAR) SCAR lipid transfer protein gene 194 3 PSY4 CAPS Spe I organ pigmentation loci 219 3 M06-CAPS CAPS Cla I Phytophthora capsici 453 5 FPACS75 CAPS Dde I ACS075 7 MS3 (AluI) CAPS Alu I genetic male sterility 412 8 BS2 (SCAR) SCAR Bacterial spot 550 9 FPSSR9 SCAR Phytophthora capsici 268 9 FPTr053 (PvuII) CAPS Pvu II Phytophthora capsici 900 9

And, a high-density gene association map of C. annuum of the present invention is shown in FIGS. 1A to 3D.

1A to 3D, markers are located on the right side of the related group and cumulative recombination distances (cM) are displayed on the left side. The SSR marker developed with the BAC sequence was displayed in blue, the web search primer was displayed in brown, and the SSR marker using cDNA library was displayed in red. SSR markers derived from SNU3 map and TMDH map are shown in green, and molecular markers related to traits are shown in pink.

Example  4 : SNU3 , TMDH map Comparison map with comparative map )

In order to verify the gene-related map prepared in Example 3, the present invention was intended to verify the present invention by preparing a SNU3 map (Yi et al ., 2006) and a comparative map, which are the most used. At this time, there was not enough marker with homology (synteny) to directly compare with SNU3 map, so it was compared with FnP(AC) map.

The sequencing was performed on gDNAs of C. annuum and C. chinense to find differences in sequencing and to determine whether the parts were cleaved with restriction enzymes, and primers cleaved with restriction enzymes were analyzed in sequencing. PCR amplification and treatment with restriction enzymes according to the polymorphism were observed (using STS marker). At this time, when polymorphism appeared, the genotypes that were analyzed and analyzed for 190 individuals in the F2 population were entered and registered in the genetic map. Then, sequencing was performed in the same way as CAPS marker development, and SNPs were identified and primers were designed based on the portion where the SNP was uniquely present (using SNP markers).

delete

Using the primers synthesized in this way, the gDNA of C. annuum and C. chinense was analyzed using the HRM (Hign resolution melting) method. At this time, Roche's LightCycler 480 was used as a device used, and LightCycler Analysis Software Applications (LC480 software Version 1.5) was used as an analysis program.

In this way, an FnP(AC) map was obtained.

As described above, homology of all linkage groups LG) was confirmed as a result of confirming the homology of the SNU3 map and the FnP(AC) map, but due to the nature of the SNU3 map, there is no LG No. 8, so the TMDH map (Minamiyama et al ., 2006) was used. It confirmed the homology of LG No. 8.

delete

delete

delete

<110> Fnp, Co. <120> SSR markers and Genetic linkage map using Intraspecific population of Capsicum annuum <130> PD13-1076 <140> 10-2012-0009961 <141> 2012-01-31 <160> 1098 <170> KopatentIn 2.0 <210> 1 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0996 Forward <400> 1 cacccttctc tgctttgtca g 21 <210> 2 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0996 Reverse <400> 2 tggggtaata tttgtccgtc a 21 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0214 Forward <400> 3 ttgcaagagt agatcgttga cc 22 <210> 4 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0214 Reverse <400> 4 tgggtctggt tgttgttctt c 21 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0890 Forward <400> 5 taagagcaag gaggctctgc 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0890 Reverse <400> 6 gacatgatcc aacccaatcc 20 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1072 Forward <400> 7 tctaataaag ctagttcttc agcg 24 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1072 Reverse <400> 8 ttgacaaatt cttccgaggg 20 <210> 9 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE137 Forward <400> 9 gaggtggtag ccgtggttat gg 22 <210> 10 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE137 Reverse <400> 10 tgcagtttgt ggcaaagtgt cc 22 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS405 Forward <400> 11 ttcttgggtc ccacactttc 20 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS405 Reverse <400> 12 aggttgaaag gagggcaata 20 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0612 Forward <400> 13 ggccattgaa gctgcttatc 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0612 Reverse <400> 14 taccccactc cactctccac 20 <210> 15 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4489 Forward <400> 15 gcagtgagaa cggtgaatct c 21 <210> 16 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4489 Reverse <400> 16 caattggtca tcgattgtgt g 21 <210> 17 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1329 Forward <400> 17 ccacacgttc attccctcta a 21 <210> 18 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1329 Reverse <400> 18 ctgcgagtct gcagaagaaa g 21 <210> 19 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2712 Forward <400> 19 atgagtgacc actccttggt g 21 <210> 20 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2712 Reverse <400> 20 gcagagaaaa ataaaggaaa actaaca 27 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1560 Forward <400> 21 ggtcctttca tgctgggtaa 20 <210> 22 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1560 Reverse <400> 22 agaactttcc tgcccatcct 20 <210> 23 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5062 Forward <400> 23 gggcatgtct gattttcttg a 21 <210> 24 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5062 Reverse <400> 24 tcctaggcag cacaaacctt 20 <210> 25 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0750 Forward <400> 25 aacaaaacgc gctaaaatgg 20 <210> 26 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0750 Reverse <400> 26 acaagtcatg ggagaatggc 20 <210> 27 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1601 Forward <400> 27 ggttatggtt tggccatttc t 21 <210> 28 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1601 Reverse <400> 28 atcaaatttc tggctttgcc t 21 <210> 29 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1478 Forward <400> 29 gcatcaacaa caacgcctaa t 21 <210> 30 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1478 Reverse <400> 30 tcagaagcaa aagggtcaga a 21 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1475 Forward <400> 31 aaagagggga gttatggcgt 20 <210> 32 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1475 Reverse <400> 32 ggtcagaagc aaaagggtca 20 <210> 33 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0023 Forward <400> 33 atggaagatg ccattgaagc 20 <210> 34 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0023 Reverse <400> 34 accgaccacc tctacctcct 20 <210> 35 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1440 Forward <400> 35 ttccaattct ccctctcagc 20 <210> 36 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1440 Reverse <400> 36 gatggggatt ggaattaggg 20 <210> 37 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE063 Forward <400> 37 ccaaatcatc atcaatgcga aga 23 <210> 38 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE063 Reverse <400> 38 cggagtagat ttggcggaga aa 22 <210> 39 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1390 Forward <400> 39 acacacgaca ggcaaaatca 20 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1390 Reverse <400> 40 aagggcgagg gttagagaag 20 <210> 41 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1198 Forward <400> 41 ggaccaaatc atcatcaatg c 21 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1198 Reverse <400> 42 agcaatccca agaaaaagct c 21 <210> 43 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0286 Forward <400> 43 aggagggatg ggtgctaact 20 <210> 44 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0286 Reverse <400> 44 ttgcaaatct tttgtcaatt ttt 23 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0204 Forward <400> 45 aagaaatggc acaagcgagt 20 <210> 46 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0204 Reverse <400> 46 tccaacatag ccactagccc 20 <210> 47 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1306 Forward <400> 47 gctcctcatt agtagccccc 20 <210> 48 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1306 Reverse <400> 48 tggacttgga caaccaatca 20 <210> 49 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2867 Forward <400> 49 tggacttgga caaccaatca t 21 <210> 50 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2867 Reverse <400> 50 cccctttatt tttcaaccca a 21 <210> 51 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE004 Forward <400> 51 tgggaagaga aattgtgaaa gca 23 <210> 52 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE004 Reverse <400> 52 caatgccaac aatggcatcc ta 22 <210> 53 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3273 Forward <400> 53 atcacctttt gctcaccctt c 21 <210> 54 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3273 Reverse <400> 54 tacatttttg gaggacgcaa c 21 <210> 55 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0184 Forward <400> 55 cacaccgggt ttgtgtgtaa 20 <210> 56 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0184 Reverse <400> 56 aagaaaaggg ggcaaactgt 20 <210> 57 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1716 Forward <400> 57 taccaagcaa aacccaaagg 20 <210> 58 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1716 Reverse <400> 58 ccaacttggt ttgaaaatga gg 22 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0154 Forward <400> 59 ttaagtttcc ttgcccatcg 20 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0154 Reverse <400> 60 aacgtcgttt gggtgattgt 20 <210> 61 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0431 Forward <400> 61 cccatcgatt ctcctcgata 20 <210> 62 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0431 Reverse <400> 62 tctctggttg ttgagcgttg 20 <210> 63 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0273 Forward <400> 63 agagtgaatt tcgcctggaa 20 <210> 64 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0273 Reverse <400> 64 gtggggttac cgagaacaga 20 <210> 65 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0483 Forward <400> 65 cgatcaccaa aattctctct ct 22 <210> 66 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0483 Reverse <400> 66 tattgggaat tgtggaggga 20 <210> 67 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0449 Forward <400> 67 tcctttgctg ttgtcgatct c 21 <210> 68 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0449 Reverse <400> 68 actcaccgga ggttcaaatg 20 <210> 69 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1589 Forward <400> 69 gggttcatcg atttatattg tacg 24 <210> 70 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1589 Reverse <400> 70 ataatgggaa ttgtggaggg a 21 <210> 71 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1731 Forward <400> 71 caacctcctt ccttcccttc 20 <210> 72 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1731 Reverse <400> 72 aaatttgaat gtggcgggta 20 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0400 Forward <400> 73 catttttccc atttcccctt 20 <210> 74 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0400 Reverse <400> 74 ccaagattcc agcttttcca 20 <210> 75 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0022 Forward <400> 75 ggagaattgg tgttacatga agg 23 <210> 76 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0022 Reverse <400> 76 aattgttgac catccccaaa 20 <210> 77 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE027 Forward <400> 77 tggagaattg gtgttacatg aagg 24 <210> 78 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE027 Reverse <400> 78 ttcggaccct tctccatcac tt 22 <210> 79 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-313 Forward <400> 79 cagcctgctt ggctagaact 20 <210> 80 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-313 Reverse <400> 80 tcgtcatgca tggctaatct 20 <210> 81 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0943 Forward <400> 81 cccctatctc tttgctgctt 20 <210> 82 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0943 Reverse <400> 82 tcttccctga cgtatttgcc 20 <210> 83 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0018 Forward <400> 83 ggaggttccc actctcacaa 20 <210> 84 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0018 Reverse <400> 84 gctcttgaca caaccccaat 20 <210> 85 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3461 Forward <400> 85 ttttccgatt ctctaatcag gttc 24 <210> 86 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3461 Reverse <400> 86 tatccgtttt gaagccatca c 21 <210> 87 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0514 Forward <400> 87 ctagtacgag gcaggggagg 20 <210> 88 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0514 Reverse <400> 88 ccagatcccg cttttgacta 20 <210> 89 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1677 Forward <400> 89 cgtgtgatat acaggcggat t 21 <210> 90 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1677 Reverse <400> 90 gtactcgcac caacctagtg g 21 <210> 91 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0794 Forward <400> 91 actcttcatg gagaagggca 20 <210> 92 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0794 Reverse <400> 92 agggaagaga gagggcactt 20 <210> 93 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0357 Forward <400> 93 aaattggaat tgaaaggggg 20 <210> 94 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0357 Reverse <400> 94 tgttggagcc atgtcagaag 20 <210> 95 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5219 Forward <400> 95 ctcggaagaa gccaaatgtc 20 <210> 96 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5219 Reverse <400> 96 tgtttgcgaa caacaccatt 20 <210> 97 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS612 Forward <400> 97 tccaccatga atcgaagaca 20 <210> 98 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS612 Reverse <400> 98 agtcgcatcc tgtccaaagt 20 <210> 99 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE034 Forward <400> 99 aaaccgcttg gtaataaagg tgct 24 <210> 100 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE034 Reverse <400> 100 ccactccagt tttgatcatc tcca 24 <210> 101 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0452 Forward <400> 101 ccgcttggta ataaaggtgc 20 <210> 102 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0452 Reverse <400> 102 ccagttttga tcatctccaa ca 22 <210> 103 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0054 Forward <400> 103 tctgggaatt ttggaactgc 20 <210> 104 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0054 Reverse <400> 104 aaattctggc tccgattgtg 20 <210> 105 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0386 Forward <400> 105 catgggtgag ggtacatggt 20 <210> 106 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0386 Reverse <400> 106 agagggaagg gttatttgcc 20 <210> 107 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1595 Forward <400> 107 ggggagaaac gaaattaggt g 21 <210> 108 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1595 Reverse <400> 108 agagggaagg gttatttgcc t 21 <210> 109 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1167 Forward <400> 109 tttggtcatt gttcaccttc a 21 <210> 110 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1167 Reverse <400> 110 agatggaata cgcggagatg 20 <210> 111 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0137 Forward <400> 111 ctcagcaaat tgttccagca 20 <210> 112 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0137 Reverse <400> 112 tcggtcattt ctatccgacc 20 <210> 113 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3304 Forward <400> 113 cccatattac cctcatttct cc 22 <210> 114 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3304 Reverse <400> 114 agccaccatc gttatgacaa g 21 <210> 115 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0799 Forward <400> 115 tctttgtccc tgctgctgta 20 <210> 116 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0799 Reverse <400> 116 accccaaaac acaaccaaaa 20 <210> 117 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0944 Forward <400> 117 ccccatatta ccctcatttc tc 22 <210> 118 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0944 Reverse <400> 118 agtagcatca gaagccggtg 20 <210> 119 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1267 Forward <400> 119 cttgtcataa cgatggtggc t 21 <210> 120 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1267 Reverse <400> 120 aactcacccc aaaacacaac c 21 <210> 121 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE145 Forward <400> 121 tgagggtatt ttcgtcattt cac 23 <210> 122 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE145 Reverse <400> 122 gaaagcggaa aacattaaga gtca 24 <210> 123 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3934 Forward <400> 123 cggacccttc tccatcactt a 21 <210> 124 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3934 Reverse <400> 124 tgggaattgg tgttacatga ag 22 <210> 125 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5243 Forward <400> 125 agggtcctat taccccgaac t 21 <210> 126 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5243 Reverse <400> 126 aaatatcccc tcgaacaatg g 21 <210> 127 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0973 Forward <400> 127 ctctccaatc cttcccttcc 20 <210> 128 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0973 Reverse <400> 128 gtgttgctgg tgctgacatt 20 <210> 129 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0778 Forward <400> 129 tgtcgacgaa caacaagagg 20 <210> 130 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0778 Reverse <400> 130 atttgcatcg gaaaatcagg 20 <210> 131 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5571 Forward <400> 131 aattgcagat gtgaaggaag c 21 <210> 132 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5571 Reverse <400> 132 gcaaagactt ttaatgaagg acaaa 25 <210> 133 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2693 Forward <400> 133 acatcaacct ctccaccaaa a 21 <210> 134 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2693 Reverse <400> 134 tcgacgagat cctgtgtaag g 21 <210> 135 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1604 Forward <400> 135 ccaccaaaat cccatttcac 20 <210> 136 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1604 Reverse <400> 136 tgttgggaat tggtttgaag 20 <210> 137 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0410 Forward <400> 137 ccaccaaaat cccatttcac 20 <210> 138 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0410 Reverse <400> 138 cgtcgacgag atcctgtgta 20 <210> 139 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1222 Forward <400> 139 atcccatttc acaatcacag g 21 <210> 140 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1222 Reverse <400> 140 gtgatggttg atgtgtgttg g 21 <210> 141 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE067 Forward <400> 141 tccaccaaaa tcccatttca ca 22 <210> 142 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE067 Reverse <400> 142 acattgccca caccaatcac tg 22 <210> 143 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1430 Forward <400> 143 ttaccttcga ggttcccaga 20 <210> 144 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1430 Reverse <400> 144 tcggacttgg atttgagctt 20 <210> 145 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5247 Forward <400> 145 caaaaactgg acaaagtttg ga 22 <210> 146 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5247 Reverse <400> 146 ttgctaggtt ttcttttctt gtg 23 <210> 147 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1557 Forward <400> 147 tgtctggcca gcaaagtcta t 21 <210> 148 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1557 Reverse <400> 148 ttatttttgg tgtgcccatg t 21 <210> 149 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4039 Forward <400> 149 agctccatca caatcatcca c 21 <210> 150 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4039 Reverse <400> 150 ctatcaccac aaagcctgca t 21 <210> 151 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5371 Forward <400> 151 aagaagaagc agaagttccc g 21 <210> 152 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5371 Reverse <400> 152 ttggttcctc acctgaatca c 21 <210> 153 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0798 Forward <400> 153 tctttcgaaa ttgtgggctt 20 <210> 154 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0798 Reverse <400> 154 tccctccaca actgatttcg 20 <210> 155 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1640 Forward <400> 155 aaaaaggtga gttcctcaaa gtg 23 <210> 156 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1640 Reverse <400> 156 ccacaactga tttcgcatac c 21 <210> 157 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1002 Forward <400> 157 ttgcaagagt agatcgttga cc 22 <210> 158 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1002 Reverse <400> 158 atgatctttg acgacgaggg 20 <210> 159 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB4998 Forward <400> 159 cgtcctgaat gtgatgaacg 20 <210> 160 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB4998 Reverse <400> 160 caatccctgc tccaatgttt 20 <210> 161 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1057 Forward <400> 161 tgaacagatt ccgatgtcca 20 <210> 162 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1057 Reverse <400> 162 ctgccacaac ttgttcctca 20 <210> 163 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0434 Forward <400> 163 ccctttgcgg agtattgaga 20 <210> 164 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0434 Reverse <400> 164 aagcggcaaa acagagaaaa 20 <210> 165 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4569 Forward <400> 165 agcagcaatt gagctattgg a 21 <210> 166 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4569 Reverse <400> 166 caggttcagc agcaataaag c 21 <210> 167 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1168 Forward <400> 167 ccactctccc aacaactcaa a 21 <210> 168 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1168 Reverse <400> 168 ggagtgccct tctgctaaag t 21 <210> 169 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2366 Forward <400> 169 ttttggattg acctttttcc c 21 <210> 170 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2366 Reverse <400> 170 tgccaatagc caaatttctc a 21 <210> 171 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1180 Forward <400> 171 cctgattgcc cctctttgta 20 <210> 172 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1180 Reverse <400> 172 aacaaactgc tgtcattttc ca 22 <210> 173 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4982 Forward <400> 173 ggattttctt ggtgagattc g 21 <210> 174 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4982 Reverse <400> 174 ccaagaaata aaacccccaa a 21 <210> 175 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2744 Forward <400> 175 cacccgttga gaggtatctg a 21 <210> 176 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2744 Reverse <400> 176 ggagaggaag gctatgaagg a 21 <210> 177 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1687 Forward <400> 177 cccaaagcaa ttgtccctaa 20 <210> 178 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1687 Reverse <400> 178 tgagaaagag gaaaacatca caa 23 <210> 179 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1551 Forward <400> 179 ggaacaggag aaaaacggct 20 <210> 180 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1551 Reverse <400> 180 gcagcgcctt gagtaaaatc 20 <210> 181 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1490 Forward <400> 181 tgggagctcc ttcatgctat 20 <210> 182 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1490 Reverse <400> 182 aagctcctgt ggtttgttgc 20 <210> 183 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0091 Forward <400> 183 gatgacgacg gggaagataa 20 <210> 184 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0091 Reverse <400> 184 agtggaaacg attcccgtag 20 <210> 185 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0502 Forward <400> 185 cgtcggagag aggagagaga 20 <210> 186 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0502 Reverse <400> 186 acaattgggg atgcagaaag 20 <210> 187 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1131 Forward <400> 187 ctctcctcca tggttgtcgt 20 <210> 188 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1131 Reverse <400> 188 tcttcaagtt gccagtcgtg 20 <210> 189 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2076 Forward <400> 189 cttgagtttc caggctctcc t 21 <210> 190 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2076 Reverse <400> 190 tcttccggcc ctttagtttt a 21 <210> 191 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3038 Forward <400> 191 aacaacagtt caatcaaccg c 21 <210> 192 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3038 Reverse <400> 192 taccaatgca tctgagtgct g 21 <210> 193 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4367 Forward <400> 193 ttgcacataa actactaata aacttgc 27 <210> 194 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4367 Reverse <400> 194 ccgaaaattg tgagtcgtga t 21 <210> 195 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1180 Forward <400> 195 cctgattgcc cctctttgta 20 <210> 196 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1180 Reverse <400> 196 aacaaactgc tgtcattttc ca 22 <210> 197 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0117 Forward <400> 197 cccaacaact caaatggctt 20 <210> 198 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0117 Reverse <400> 198 taagtggagg tgcccttctg 20 <210> 199 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2213 Forward <400> 199 gatctagcct atctggacgc c 21 <210> 200 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2213 Reverse <400> 200 tttaaccaaa aaggcaaatc tagt 24 <210> 201 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0902 Forward <400> 201 atctggacgc caaagctaga 20 <210> 202 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0902 Reverse <400> 202 ctttttaacc aaaaaggcaa atc 23 <210> 203 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1148 Forward <400> 203 tattctgggt tggatgggaa 20 <210> 204 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1148 Reverse <400> 204 tcaaatcgca caaaattgga 20 <210> 205 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1303 Forward <400> 205 ggcgaaggag atgaaagaga 20 <210> 206 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1303 Reverse <400> 206 aagaacgtgt gtgagttttc ca 22 <210> 207 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0426 Forward <400> 207 ccaaagggtc atcaaaatcc t 21 <210> 208 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0426 Reverse <400> 208 tcacgttcac gttaaactcc c 21 <210> 209 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2879 Forward <400> 209 ataatatcgg gcctgttgtc c 21 <210> 210 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2879 Reverse <400> 210 cacgggcttg tcaataacag t 21 <210> 211 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2942 Forward <400> 211 cacgggcttg tcaataacag t 21 <210> 212 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2942 Reverse <400> 212 gtccaagcta ttgaggaagg g 21 <210> 213 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PH5-SCAR Forward <400> 213 ctttcccata tagcccactt gc 22 <210> 214 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PH5-SCAR Reverse <400> 214 cgaaccaacg ttggcaactc c 21 <210> 215 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0178 Forward <400> 215 gaagctatga gggatgctgg 20 <210> 216 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0178 Reverse <400> 216 aacaatggct tacagggctc 20 <210> 217 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1142 Forward <400> 217 tacagtgaaa tcaatggcgg 20 <210> 218 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1142 Reverse <400> 218 ccttttccca aacccaaaat 20 <210> 219 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1143 Forward <400> 219 cgggtcgtag tcaagaacaa g 21 <210> 220 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1143 Reverse <400> 220 atggacgcat agttcgcttc 20 <210> 221 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0317 Forward <400> 221 atgggcacaa atagccaaac 20 <210> 222 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0317 Reverse <400> 222 tggttgatga tggcgataaa 20 <210> 223 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0319 Forward <400> 223 atgtcgctcg caatttcact 20 <210> 224 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0319 Reverse <400> 224 cgtagggagg agcgatagag 20 <210> 225 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4921 Forward <400> 225 accaagaagg ttcccatcac t 21 <210> 226 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4921 Reverse <400> 226 ttgctttgtt ggtctctgct t 21 <210> 227 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4958 Forward <400> 227 ttgctttgtt ggtctctgct t 21 <210> 228 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4958 Reverse <400> 228 accaagaagg ttcccatcac t 21 <210> 229 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0574 Forward <400> 229 ggcgatttcc aattcatcac 20 <210> 230 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0574 Reverse <400> 230 cttaaaggat ccaacgctcg 20 <210> 231 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1483 Forward <400> 231 ttttcttccc ctgcctttct 20 <210> 232 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1483 Reverse <400> 232 aaaaatcttc aagcggcaaa 20 <210> 233 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2489 Forward <400> 233 ccaagtatct tgaatcaatc aacc 24 <210> 234 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2489 Reverse <400> 234 agaatacatt gccgacgatt g 21 <210> 235 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1314 Forward <400> 235 tcaccgtctt ccgtcagatt 20 <210> 236 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1314 Reverse <400> 236 caacggcgag taccttgaat 20 <210> 237 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3507 Forward <400> 237 ccatttcact tatgcccttc a 21 <210> 238 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3507 Reverse <400> 238 ggcttaactc cctggaacaa c 21 <210> 239 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1171 Forward <400> 239 gtgaatctat tttcctccgg c 21 <210> 240 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1171 Reverse <400> 240 cacatcggga tgacaaactc t 21 <210> 241 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE142 Forward <400> 241 agattccggc agtccggtta ct 22 <210> 242 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE142 Reverse <400> 242 tccggtaagc tgccttgatc tc 22 <210> 243 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0512 Forward <400> 243 atctattttc ctccggcgac 20 <210> 244 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0512 Reverse <400> 244 cggtaagctg ccttgatctc 20 <210> 245 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1147 Forward <400> 245 ccttctttgg acacgcaga 19 <210> 246 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1147 Reverse <400> 246 ggtcgacgct gtttttaagc 20 <210> 247 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE123 Forward <400> 247 cggtagctgt cgtgttcttg ag 22 <210> 248 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE123 Reverse <400> 248 tcaatacagg aggcaaacat gg 22 <210> 249 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0821 Forward <400> 249 tgcaatagca aaatacaagt gaaaa 25 <210> 250 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0821 Reverse <400> 250 ccgtattgtg ccatctcctt 20 <210> 251 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0181 Forward <400> 251 caaggctcac acagcattca 20 <210> 252 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0181 Reverse <400> 252 taaatctccc atggctcctg 20 <210> 253 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0011 Forward <400> 253 tccaaaggca atttctggac 20 <210> 254 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0011 Reverse <400> 254 ctttggcagt gtatcagcga 20 <210> 255 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1202 Forward <400> 255 ctccgtttcc gccttaaaat 20 <210> 256 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1202 Reverse <400> 256 gggaaagatg ggccataaat 20 <210> 257 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4456 Forward <400> 257 tacaaagcca gaattggcat c 21 <210> 258 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4456 Reverse <400> 258 agggcaatta cctttgccta a 21 <210> 259 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3607 Forward <400> 259 caggttcgtt ttagggcttt t 21 <210> 260 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3607 Reverse <400> 260 tggtatgatc gcatcctcct 20 <210> 261 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0017 Forward <400> 261 acccttctct ttctttttcc 20 <210> 262 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0017 Reverse <400> 262 atctgcactg ctgtgagcaa 20 <210> 263 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3872 Forward <400> 263 aattggggga gtcgacttag a 21 <210> 264 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3872 Reverse <400> 264 ggagcagtac tcctttttcc g 21 <210> 265 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0955 Forward <400> 265 gacacgaaaa gccgaaagag 20 <210> 266 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0955 Reverse <400> 266 tttggctcga gctttcactt 20 <210> 267 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1214 Forward <400> 267 aatctcattt caccatcacc g 21 <210> 268 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1214 Reverse <400> 268 aaaatcccat cacgaaattc a 21 <210> 269 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE090 Forward <400> 269 caccggccgt ttaaactttc ac 22 <210> 270 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE090 Reverse <400> 270 gctggagttt ccaaaggaca aa 22 <210> 271 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1150 Forward <400> 271 gtaacgcccc aaaagatcaa 20 <210> 272 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1150 Reverse <400> 272 gtgcccatga cccatatttc 20 <210> 273 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0707 Forward <400> 273 gtcagggacc aaggttcaga 20 <210> 274 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0707 Reverse <400> 274 gaatggtatt gcagcaagca 20 <210> 275 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1010 Forward <400> 275 tcatggaagg aaggatgacc 20 <210> 276 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1010 Reverse <400> 276 gaccagtgaa cggttggtct 20 <210> 277 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1702 Forward <400> 277 cctgcttctc caattcctct c 21 <210> 278 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1702 Reverse <400> 278 ttgtcgaccc tgcgtacata 20 <210> 279 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1013 Forward <400> 279 cagaggaagc tcctcaaggt t 21 <210> 280 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1013 Reverse <400> 280 gaatggtatt gcagcaagca t 21 <210> 281 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0426 Forward <400> 281 gggatgccag gtaatagggt 20 <210> 282 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0426 Reverse <400> 282 attcctcgga ttttgcattg 20 <210> 283 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0935 Forward <400> 283 aatgcaaagt ggatcttcgg 20 <210> 284 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0935 Reverse <400> 284 catccattta ccaaaaacca aaa 23 <210> 285 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0487 Forward <400> 285 cgcctccgac ttgaactaag 20 <210> 286 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0487 Reverse <400> 286 tcagccaaac aaaacaaata tga 23 <210> 287 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1029 Forward <400> 287 accactgcct ctctactgcg 20 <210> 288 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1029 Reverse <400> 288 tgtttcttcg ttcgcttcct 20 <210> 289 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0307 Forward <400> 289 gaaggagagt tcacaaggca ac 22 <210> 290 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0307 Reverse <400> 290 agaagcggaa gagcttgaat c 21 <210> 291 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1235 Forward <400> 291 gcttataccc ctggcaggtt 20 <210> 292 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1235 Reverse <400> 292 aaaaatggca ccaacaatcg 20 <210> 293 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0274 Forward <400> 293 ctggtggtag ttggttggct 20 <210> 294 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0274 Reverse <400> 294 ttgaaattca tcggcacaaa 20 <210> 295 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2497 Forward <400> 295 gagtatgcaa atactggggc a 21 <210> 296 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2497 Reverse <400> 296 ccaacaatcg gtaattgaag c 21 <210> 297 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0749 Forward <400> 297 cctaactaag agtgcggggg 20 <210> 298 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0749 Reverse <400> 298 cgacagccat actcacgcta 20 <210> 299 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2363 Forward <400> 299 cagtccaact gcctctgtct c 21 <210> 300 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2363 Reverse <400> 300 ttccaaatgc tttcctgaga a 21 <210> 301 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE010 Forward <400> 301 ctgtttgcca atcaccatca gg 22 <210> 302 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE010 Reverse <400> 302 gctattttcc ggcgtgtgag ag 22 <210> 303 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4586 Forward <400> 303 aactgtttgc caatcaccat c 21 <210> 304 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4586 Reverse <400> 304 gatatgcaag gtcagttccc a 21 <210> 305 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3124 Forward <400> 305 tgctgctact cccaattcta a 21 <210> 306 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3124 Reverse <400> 306 gaaatgcaaa gtggatcttc g 21 <210> 307 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4407 Forward <400> 307 gcgcaataat gaaggaagtt g 21 <210> 308 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4407 Reverse <400> 308 ctctcactgt tagtcgccgt c 21 <210> 309 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1711 Forward <400> 309 gctaaaaagc aaagtgcatc g 21 <210> 310 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1711 Reverse <400> 310 gtttgtttct ccgaatcctc c 21 <210> 311 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3219 Forward <400> 311 gaaacaagga acaaaaggga aa 22 <210> 312 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3219 Reverse <400> 312 aacggagacc caaactcaaa g 21 <210> 313 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1271 Forward <400> 313 gccttctttt tcatctttcc c 21 <210> 314 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1271 Reverse <400> 314 ctggcaaccc aagtcttagc 20 <210> 315 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0019 Forward <400> 315 caaagtgcat cgactttcca 20 <210> 316 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0019 Reverse <400> 316 gctctgtcat ctcctgctcc 20 <210> 317 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0380 Forward <400> 317 catcaacaaa accacattcc c 21 <210> 318 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0380 Reverse <400> 318 ctggcaaccc aagtcttagc 20 <210> 319 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0519 Forward <400> 319 tatgcctaca gcgacaacca 20 <210> 320 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0519 Reverse <400> 320 ccctcaagaa ttccctccat 20 <210> 321 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-101 Forward <400> 321 tcagcaatta acatgccaaa a 21 <210> 322 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-101 Reverse <400> 322 tggattggga gaagatcgac 20 <210> 323 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0501 Forward <400> 323 cgtatttcct cttaacccta gcaa 24 <210> 324 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0501 Reverse <400> 324 caatacagat cacgtgtggt aga 23 <210> 325 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1644 Forward <400> 325 gttggagatt tgttaaagtc aagga 25 <210> 326 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1644 Reverse <400> 326 acagatcacg tgtggtagat gt 22 <210> 327 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1266 Forward <400> 327 acacgccaag aaaatcatct c 21 <210> 328 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1266 Reverse <400> 328 tgagccattg ctgaagaaaa t 21 <210> 329 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6508 Forward <400> 329 cttctgaatc tcgtggcaaa g 21 <210> 330 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6508 Reverse <400> 330 agtcactgct actccctccg t 21 <210> 331 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0327 Forward <400> 331 gtgacagctt tgcttgcttg 20 <210> 332 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0327 Reverse <400> 332 tacaagtctt gccaacagcg 20 <210> 333 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1562 Forward <400> 333 tttacattgt cgtacccaaa aa 22 <210> 334 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1562 Reverse <400> 334 gttgcgcctc gagtttctaa 20 <210> 335 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0553 Forward <400> 335 tcagcatacg tgaaagtcgg 20 <210> 336 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0553 Reverse <400> 336 ctctcgtcct catcctcgtc 20 <210> 337 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0478 Forward <400> 337 cgaatgctgg atatgggatt 20 <210> 338 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0478 Reverse <400> 338 gagggcacaa atgcaaagat 20 <210> 339 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1342 Forward <400> 339 tcttggtggc acaagtgaag 20 <210> 340 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1342 Reverse <400> 340 tcagcttacg ttcacctccc 20 <210> 341 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4514 Forward <400> 341 aaaaacggat gaacagtaaa gtca 24 <210> 342 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4514 Reverse <400> 342 gagagaggag agagatcggg a 21 <210> 343 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> HpmsE008 Forward <400> 343 ccccttaact tttaattcta gatctgc 27 <210> 344 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE008 Reverse <400> 344 tcgttgttcc tccatcacct ca 22 <210> 345 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0010 Forward <400> 345 ttaaaaccca gtgcctgtcc 20 <210> 346 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0010 Reverse <400> 346 agtctcgacc ttccacctcc 20 <210> 347 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1370 Forward <400> 347 ctatttgctg cagccctagc 20 <210> 348 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1370 Reverse <400> 348 ccagctgaag tagtcctcgg 20 <210> 349 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE053 Forward <400> 349 ttcaaagaat ccagagactt caca 24 <210> 350 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE053 Reverse <400> 350 ttcatgcaat tccaaagtct cca 23 <210> 351 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0220 Forward <400> 351 gtagcgtttg ttcccgttgt 20 <210> 352 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0220 Reverse <400> 352 aaatggagca tcgtggactc 20 <210> 353 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1300 Forward <400> 353 ggcagtcctt caagaagtcg 20 <210> 354 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1300 Reverse <400> 354 tggaccttga tggtgacgta 20 <210> 355 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0915 Forward <400> 355 tatgcactcc acagtccacg 20 <210> 356 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0915 Reverse <400> 356 aaaaccggaa aacgatttga 20 <210> 357 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0916 Forward <400> 357 cctttttgat tgcgctgata 20 <210> 358 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0916 Reverse <400> 358 caaagagcac gccagaaaat 20 <210> 359 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1785 Forward <400> 359 tttgcaatgt ctttgttgcc 20 <210> 360 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1785 Reverse <400> 360 agaatgcaac tcttcaactt ttt 23 <210> 361 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5464 Forward <400> 361 gacagttttg atttttgccc a 21 <210> 362 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5464 Reverse <400> 362 tcctcttgtc ttcgtctcca a 21 <210> 363 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> PSY4 Forward <400> 363 ccaaattgaa tagtgatcct ggt 23 <210> 364 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PSY4 Reverse <400> 364 tcaaaggtac ttgcgcattt t 21 <210> 365 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4395 Forward <400> 365 gatttcttcc aaaaactcaa caa 23 <210> 366 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4395 Reverse <400> 366 ttgggtgatt ttggtcatca t 21 <210> 367 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4490 Forward <400> 367 gggatgtaaa agccaatgag c 21 <210> 368 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4490 Reverse <400> 368 tggaggagga atgtgaaact g 21 <210> 369 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1414 Forward <400> 369 tgtctcctac cccctttcaa 20 <210> 370 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1414 Reverse <400> 370 gggatctgct tttgttggtg 20 <210> 371 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0334 Forward <400> 371 tccatcttct tccagccttc 20 <210> 372 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0334 Reverse <400> 372 tcgggctaga aaaaggaaaa 20 <210> 373 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5560 Forward <400> 373 tttttcagca taatcttttg gat 23 <210> 374 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5560 Reverse <400> 374 tggtcttcct catgctcatt t 21 <210> 375 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0017 Forward <400> 375 ccttaaccct gatctgcatc ttga 24 <210> 376 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0017 Reverse <400> 376 ggccatcaaa atagaaaaat gcaa 24 <210> 377 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0548 Forward <400> 377 taccaagtat cccccttccc 20 <210> 378 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0548 Reverse <400> 378 aagtccttcc acattgtgcc 20 <210> 379 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1353 Forward <400> 379 tcgtctttca cttgtctttt gttc 24 <210> 380 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1353 Reverse <400> 380 cccgtccttt cgagaagagt 20 <210> 381 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0522 Forward <400> 381 ctccttcaag gtccagatcg 20 <210> 382 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0522 Reverse <400> 382 tgcgtctttt tgggctttta 20 <210> 383 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0877 Forward <400> 383 ttaccggtta ctacaggccg 20 <210> 384 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0877 Reverse <400> 384 caagcttaaa attaaaaagc aagga 25 <210> 385 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3341 Forward <400> 385 tcattatcac catcatccat tg 22 <210> 386 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3341 Reverse <400> 386 ggtgatcatt tcagctggtg 20 <210> 387 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1042 Forward <400> 387 atgtgaccct tctctcccaa 20 <210> 388 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1042 Reverse <400> 388 agcagcgaaa aatccagaaa 20 <210> 389 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2523 Forward <400> 389 ttgaagtacc tccaaaatgc c 21 <210> 390 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2523 Reverse <400> 390 tgcgagatcc agtaagcttg t 21 <210> 391 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPTr055(SCAR) Forward <400> 391 tttcttggag ttaggtcatc agc 23 <210> 392 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPTr055(SCAR) Reverse <400> 392 tgagtaattt ttggggacga 20 <210> 393 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5421 Forward <400> 393 gtccgcaaaa attactcatg c 21 <210> 394 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5421 Reverse <400> 394 ggtcagattt tgaaatgggg t 21 <210> 395 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1350 Forward <400> 395 cacaaagaaa caaaagaggg g 21 <210> 396 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1350 Reverse <400> 396 aggggatgca taaagcaact t 21 <210> 397 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0880 Forward <400> 397 ttattaaggc cttttgcgtg a 21 <210> 398 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0880 Reverse <400> 398 aattttcaac caaccccaca 20 <210> 399 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0406 Forward <400> 399 ccccttccaa aaactaactc c 21 <210> 400 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0406 Reverse <400> 400 gagtggggat ttgggtaaag a 21 <210> 401 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3830 Forward <400> 401 ttttccgttt caacatcttc g 21 <210> 402 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3830 Reverse <400> 402 aggaagaagc aggtgaggaa g 21 <210> 403 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1114 Forward <400> 403 ggcatcagca tgtttcctct 20 <210> 404 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1114 Reverse <400> 404 tctgctgatt ctgtcatggg 20 <210> 405 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1696 Forward <400> 405 atcatcttca ttggcttccg 20 <210> 406 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1696 Reverse <400> 406 caaccccact ttcttctcca 20 <210> 407 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2977 Forward <400> 407 tctccaaatt ttgttgatcc g 21 <210> 408 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2977 Reverse <400> 408 gaacggacag caataagcaa g 21 <210> 409 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5446 Forward <400> 409 ttgaagctag aaaccctttc ac 22 <210> 410 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5446 Reverse <400> 410 cccagatgat gaagacgaaa g 21 <210> 411 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0731 Forward <400> 411 tacgtggcat ctgcaaaatc 20 <210> 412 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0731 Reverse <400> 412 tgaaaagcta acccctgcat 20 <210> 413 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1173 Forward <400> 413 gaacattcaa aggacatata tggaa 25 <210> 414 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1173 Reverse <400> 414 tccaaaacaa ggaatgcaaa 20 <210> 415 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0001 Forward <400> 415 gggtggttgt gcttgaagat 20 <210> 416 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0001 Reverse <400> 416 cggttccaca ataatggtaa a 21 <210> 417 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0586 Forward <400> 417 gcaaatgagg agaattgaac c 21 <210> 418 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0586 Reverse <400> 418 caccctaaat caaaccacaa gc 22 <210> 419 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4340 Forward <400> 419 cagatcagaa gcaaaggcaa g 21 <210> 420 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4340 Reverse <400> 420 ttcaaaaatg aaatcttcaa gaaa 24 <210> 421 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1567 Forward <400> 421 aagctgaatg gtgggagaga 20 <210> 422 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1567 Reverse <400> 422 cagatcacgc ctgaaatcct 20 <210> 423 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5826 Forward <400> 423 aggtgatggt caacaacttc g 21 <210> 424 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5826 Reverse <400> 424 tagttcacgg ggtacttgtg c 21 <210> 425 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0356 Forward <400> 425 cacaaatccc ctctttgcat 20 <210> 426 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0356 Reverse <400> 426 gggggtggat tcttgaaaa 19 <210> 427 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3606 Forward <400> 427 ctgggacttg caatcctaaa a 21 <210> 428 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3606 Reverse <400> 428 ctttgatgca ttttgaggga a 21 <210> 429 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2627 Forward <400> 429 ctgctgctaa gaaagcgaag a 21 <210> 430 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2627 Reverse <400> 430 aacagtgaaa gagcacaagt ca 22 <210> 431 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1076 Forward <400> 431 catccaagct cgacttcaca 20 <210> 432 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1076 Reverse <400> 432 cccccttcca ttgaagtacc 20 <210> 433 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4627 Forward <400> 433 cgaattgaga ggggattctt c 21 <210> 434 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4627 Reverse <400> 434 acagttgaga cgccgaattt a 21 <210> 435 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4118 Forward <400> 435 caatccccat gttgcttatt g 21 <210> 436 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4118 Reverse <400> 436 gtacgagaat ttccagcggt t 21 <210> 437 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3903 Forward <400> 437 acgagatcgt ctgcatcaaa g 21 <210> 438 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3903 Reverse <400> 438 aattcaggga tatttttaac cttttt 26 <210> 439 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-885 Forward <400> 439 aacgaaaaac aaacccaatc a 21 <210> 440 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> CAMS-885 Reverse <400> 440 ttgaaattgc tgaaactctg aa 22 <210> 441 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6678 Forward <400> 441 tatccttgag cacaaagtgc c 21 <210> 442 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6678 Reverse <400> 442 tgggacttgg aattcttact ttg 23 <210> 443 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> CAMS-237 Forward <400> 443 ccttcccttg aaattgatat tttac 25 <210> 444 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-237 Reverse <400> 444 tcccctatca ctcacatctg c 21 <210> 445 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5601 Forward <400> 445 ttcacttttc taaggatatt ccca 24 <210> 446 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5601 Reverse <400> 446 cattgggttt tgcatcattt t 21 <210> 447 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2625 Forward <400> 447 ccccctttct ctttcttctg a 21 <210> 448 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2625 Reverse <400> 448 atcgccaagg acgataactt t 21 <210> 449 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2723 Forward <400> 449 atgggttttc attttcttgg g 21 <210> 450 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2723 Reverse <400> 450 tcactcacat aaccctccca g 21 <210> 451 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0818 Forward <400> 451 atggcagcag ctatggattt 20 <210> 452 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0818 Reverse <400> 452 atttccgtgg aagtggaaca 20 <210> 453 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1434 Forward <400> 453 ttcaaagcca ggtgttttca 20 <210> 454 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1434 Reverse <400> 454 caaccccttt ttctcctaat ca 22 <210> 455 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0612 Forward <400> 455 gccaggtgtt ttcagaatcg 20 <210> 456 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0612 Reverse <400> 456 gctgctgcca ttacctaaaa ag 22 <210> 457 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1532 Forward <400> 457 atggaagcac ttgaaccatt t 21 <210> 458 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1532 Reverse <400> 458 gatttgggaa ttcatttccg t 21 <210> 459 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0328 Forward <400> 459 atcgggtcaa ccatctctca 20 <210> 460 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0328 Reverse <400> 460 cagaagaatc gccacagaca 20 <210> 461 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0936 Forward <400> 461 ggaggagtta attcatcggt ttc 23 <210> 462 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0936 Reverse <400> 462 gagtggtgtg ctctttgaag c 21 <210> 463 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1648 Forward <400> 463 cagggaacct tcgagaaaaa 20 <210> 464 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1648 Reverse <400> 464 gtgcctggtg ttgtatgtgc 20 <210> 465 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4976 Forward <400> 465 tgataatgga taggtgggtc c 21 <210> 466 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4976 Reverse <400> 466 accatctctc cctctctccc t 21 <210> 467 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3284 Forward <400> 467 catcacatcc ctttgattgc t 21 <210> 468 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3284 Reverse <400> 468 caacaagcca catcaagtcc t 21 <210> 469 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4711 Forward <400> 469 gagaaagctg gcaaatgtct g 21 <210> 470 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4711 Reverse <400> 470 ggcaaatcaa tagtgacctt aagaa 25 <210> 471 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0708 Forward <400> 471 ccaggtggag gacagaagaa 20 <210> 472 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0708 Reverse <400> 472 tttgagtgat accctggcaa 20 <210> 473 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0710 Forward <400> 473 cggaagacac ttcagttcca g 21 <210> 474 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0710 Reverse <400> 474 atcccttctt ctccttcccc 20 <210> 475 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1759 Forward <400> 475 cattttcgaa tcccacgagt 20 <210> 476 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1759 Reverse <400> 476 tcaaaagcac ctgttcacaa a 21 <210> 477 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0760 Forward <400> 477 tcccacgagt ctttttgagg 20 <210> 478 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0760 Reverse <400> 478 aaagggaatt atggggttgg 20 <210> 479 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1585 Forward <400> 479 ttgcaaaaag atgctttgct t 21 <210> 480 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1585 Reverse <400> 480 ttgggacatt gcaaaagttt c 21 <210> 481 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE015 Forward <400> 481 ttgtgagggt ttgacactgg ga 22 <210> 482 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE015 Reverse <400> 482 ccgagctcga tgaggatgaa ct 22 <210> 483 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3363 Forward <400> 483 ggatacacag tgacgccatt t 21 <210> 484 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3363 Reverse <400> 484 aaaacaaaga ccaacccgaa c 21 <210> 485 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1014 Forward <400> 485 atccaaacac caatggaagc 20 <210> 486 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1014 Reverse <400> 486 ggtggctgaa ttgcttcttc 20 <210> 487 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1015 Forward <400> 487 cggtgaaggt gatgatgatg 20 <210> 488 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1015 Reverse <400> 488 ggtggctgaa ttgcttcttc 20 <210> 489 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1494 Forward <400> 489 aattcgccgt catcgacc 18 <210> 490 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1494 Reverse <400> 490 aacggaacac gaaggaactg 20 <210> 491 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3521 Forward <400> 491 aggtgaggag gagtggtcat t 21 <210> 492 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3521 Reverse <400> 492 tttgagccac agataattgc c 21 <210> 493 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5701 Forward <400> 493 aagtcctcca gagaaacagc c 21 <210> 494 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5701 Reverse <400> 494 attgtctcca ttgtttgcac c 21 <210> 495 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1166 Forward <400> 495 tccaaaggga gacattgagc 20 <210> 496 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1166 Reverse <400> 496 tcagcgaaat caagatggtg 20 <210> 497 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1728 Forward <400> 497 ggttgcctcc atgttgagta a 21 <210> 498 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1728 Reverse <400> 498 gaaacgtaac cgggtcaaaa 20 <210> 499 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1179 Forward <400> 499 gacccgatcc ccaatctaac 20 <210> 500 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1179 Reverse <400> 500 cgtttctctt gcagggattc 20 <210> 501 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0404 Forward <400> 501 cgatctcatt tgccctgatt 20 <210> 502 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0404 Reverse <400> 502 tgaagaaacg taaccgggtc 20 <210> 503 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS319 Forward <400> 503 tcaccttcca cagcatcaag 20 <210> 504 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS319 Reverse <400> 504 caaacgcaaa caccaatcag 20 <210> 505 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M05SSR2-CAPS Forward <400> 505 gtgcaacaga accgatacca 20 <210> 506 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> M05SSR2-CAPS Reverse <400> 506 agcgacgaag ccttagattg 20 <210> 507 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0226 Forward <400> 507 tactgccacg tcagtttcca 20 <210> 508 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0226 Reverse <400> 508 ggtgagagga gacgaaatga g 21 <210> 509 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2764 Forward <400> 509 agaaagcata attgggttgg g 21 <210> 510 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2764 Reverse <400> 510 actgtgattg tggctggaaa g 21 <210> 511 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1924 Forward <400> 511 tgttttgatg gatggttgga t 21 <210> 512 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1924 Reverse <400> 512 tgaaaacctc atcagaaatc ctt 23 <210> 513 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0401 Forward <400> 513 aaatcttatc gtcaaacttt gtcac 25 <210> 514 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0401 Reverse <400> 514 cccatttcag gcaacattaa a 21 <210> 515 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2819 Forward <400> 515 ggtctgtgca ttttggtcct a 21 <210> 516 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2819 Reverse <400> 516 agctgactcg gacatcactg t 21 <210> 517 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1237 Forward <400> 517 tctggtctca ggttgctcct 20 <210> 518 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1237 Reverse <400> 518 accccattta gctgcctctt 20 <210> 519 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1598 Forward <400> 519 tggtcaatgg ttggataatg a 21 <210> 520 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1598 Reverse <400> 520 cagacacgtc catgaacaca 20 <210> 521 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0787 Forward <400> 521 tctctctcta catctctccg ttga 24 <210> 522 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0787 Reverse <400> 522 actcccatac ggtgtcgttc 20 <210> 523 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE116 Forward <400> 523 catctctccg ttgaatctat ttcc 24 <210> 524 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE116 Reverse <400> 524 acggtcatcc attagaaccg ta 22 <210> 525 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1635 Forward <400> 525 gcgtttcact cgaacaacac t 21 <210> 526 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1635 Reverse <400> 526 aacggtggga tgaatggtta 20 <210> 527 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0100 Forward <400> 527 gcgtttcact cgaacaacac 20 <210> 528 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0100 Reverse <400> 528 tgcagcttgt caatagccac 20 <210> 529 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3029 Forward <400> 529 accctacaag ccttaatccc a 21 <210> 530 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3029 Reverse <400> 530 aagtcaaatg gcatgttttg c 21 <210> 531 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0974 Forward <400> 531 tttatcctcg ccggaactct 20 <210> 532 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0974 Reverse <400> 532 gacggtcttt cgtgttggtt 20 <210> 533 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0289 Forward <400> 533 agggccatct ctccatcatt 20 <210> 534 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0289 Reverse <400> 534 cgagggactt ccttgtagca 20 <210> 535 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-020 Forward <400> 535 cagcagtaac agaggcaggt c 21 <210> 536 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> CAMS-020 Reverse <400> 536 cacaagtgag tttattcata tcacca 26 <210> 537 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1371 Forward <400> 537 actcctcccc tttatgtcac 20 <210> 538 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1371 Reverse <400> 538 tggtggattt ttcggtatcc 20 <210> 539 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3505 Forward <400> 539 gcaatcaaca aggagagacc a 21 <210> 540 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3505 Reverse <400> 540 aacatgctgt ttctgaaggg a 21 <210> 541 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4382 Forward <400> 541 tgatataagc tttcttcctc ttcc 24 <210> 542 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4382 Reverse <400> 542 tgcaattaaa caaaacaaat tttca 25 <210> 543 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3803 Forward <400> 543 ttggatacaa acacatgaac ca 22 <210> 544 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3803 Reverse <400> 544 ccaacgacca attctctaag at 22 <210> 545 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1713 Forward <400> 545 gctcggagga tatcgaaaca 20 <210> 546 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1713 Reverse <400> 546 ttccttcctc attgtcgaat c 21 <210> 547 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5239 Forward <400> 547 cattgtcctt aaagatattt catcg 25 <210> 548 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5239 Reverse <400> 548 cttggactgc ttcaccaaaa g 21 <210> 549 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2217 Forward <400> 549 cagcatcatt cttgccttca 20 <210> 550 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2217 Reverse <400> 550 ggcattgtgg gtacaaaggt 20 <210> 551 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2837 Forward <400> 551 tttccacacg cgactcttt 19 <210> 552 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2837 Reverse <400> 552 tttttaatat aggccaagaa atatgc 26 <210> 553 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-361 Forward <400> 553 ttggtgtggt taggggagag 20 <210> 554 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-361 Reverse <400> 554 ggcgttcgaa cttgtgaaat 20 <210> 555 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0166 Forward <400> 555 tcaacccacc ttcatcaaca 20 <210> 556 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0166 Reverse <400> 556 cgagtaggga agagcattgg 20 <210> 557 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE072 Forward <400> 557 gctcatcaac ccaccttcat ca 22 <210> 558 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE072 Reverse <400> 558 atgcgttgtc cgagtaggga ag 22 <210> 559 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0433 Forward <400> 559 ccccaaccac tccttgacta 20 <210> 560 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0433 Reverse <400> 560 caacgagatt aacatggcga 20 <210> 561 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6406 Forward <400> 561 ggccatcgga gaaagatttt a 21 <210> 562 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6406 Reverse <400> 562 ggatgttact ggtggtggat g 21 <210> 563 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2720 Forward <400> 563 ctccaaatgt ttcctcgatg t 21 <210> 564 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2720 Reverse <400> 564 accgtcaggc gtaagaaatt a 21 <210> 565 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1223 Forward <400> 565 ggacacacag agcagcttga 20 <210> 566 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1223 Reverse <400> 566 gacctcattg gtagcccaga 20 <210> 567 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4429 Forward <400> 567 acaggacaca cagagcagct t 21 <210> 568 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4429 Reverse <400> 568 acttggattt agcacggacc t 21 <210> 569 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0053 Forward <400> 569 catgaatgcg gtgtggttta 20 <210> 570 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0053 Reverse <400> 570 ggaatcttta atggaatgaa ggaa 24 <210> 571 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3034 Forward <400> 571 cttcacaagc tgtgggatga t 21 <210> 572 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3034 Reverse <400> 572 ttcccagatc gaacagacat c 21 <210> 573 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1552 Forward <400> 573 caagctcctc tcctcagtgc 20 <210> 574 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1552 Reverse <400> 574 gtaatcggtc tgggtttgga 20 <210> 575 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5206 Forward <400> 575 ctgttggtgc agattccaga t 21 <210> 576 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5206 Reverse <400> 576 gtaacataag ccttctggcc c 21 <210> 577 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0733 Forward <400> 577 tagcctgttg tgaggtgtgg 20 <210> 578 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0733 Reverse <400> 578 acacgaatgc tgttgctgtc 20 <210> 579 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1396 Forward <400> 579 atggcgtcct catcttcatc 20 <210> 580 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1396 Reverse <400> 580 atgaagggat cgtaaacccc 20 <210> 581 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0171 Forward <400> 581 tttttccctc actaaggggc 20 <210> 582 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0171 Reverse <400> 582 gaatcagggc aaggattgaa 20 <210> 583 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0593 Forward <400> 583 gcacggctct atatctctct atctt 25 <210> 584 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0593 Reverse <400> 584 tcaacatctc accgaagctg 20 <210> 585 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> HpmsE002 Forward <400> 585 gcacggctct atatctctct atctt 25 <210> 586 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE002 Reverse <400> 586 ggaacgaaat cgatcaacat ct 22 <210> 587 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1491 Forward <400> 587 tgcgaaaccg tacgtaacaa 20 <210> 588 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1491 Reverse <400> 588 atgggaagtg tcttcatgcc 20 <210> 589 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4583 Forward <400> 589 acgtaacaac catccaagca g 21 <210> 590 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4583 Reverse <400> 590 attggaagat ggagaaggga a 21 <210> 591 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3292 Forward <400> 591 cgcgactgga tctgttaagt c 21 <210> 592 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3292 Reverse <400> 592 ggccaccata acttcatctt tt 22 <210> 593 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1127 Forward <400> 593 atctttgcct cttcttttgc c 21 <210> 594 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1127 Reverse <400> 594 cccacaacct caaatgtgaa t 21 <210> 595 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2177 Forward <400> 595 ctttttcttc ccctttccct t 21 <210> 596 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2177 Reverse <400> 596 ggatcaacaa ggagctgtga a 21 <210> 597 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1257 Forward <400> 597 gtgaatcagc ccacgaattt 20 <210> 598 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1257 Reverse <400> 598 gagacaccgg tccaatgagt 20 <210> 599 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5722 Forward <400> 599 attctcttcc accgcctttt 20 <210> 600 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5722 Reverse <400> 600 gaagcatctg agcaagaccc 20 <210> 601 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1195 Forward <400> 601 ccgccttttc ttccctatct a 21 <210> 602 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1195 Reverse <400> 602 cgttaaagca ccatttccgt a 21 <210> 603 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1188 Forward <400> 603 gaggaaaaag gggtgatggt 20 <210> 604 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1188 Reverse <400> 604 ggacgatgag gagcttgaaa 20 <210> 605 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0060 Forward <400> 605 ttcggcaacc ctagaagaga 20 <210> 606 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0060 Reverse <400> 606 cacagctttt atgcgttgga 20 <210> 607 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE014 Forward <400> 607 ctttggaaca tttctttggg gg 22 <210> 608 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE014 Reverse <400> 608 gcggacgtag cagtaggttt gg 22 <210> 609 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0360 Forward <400> 609 cacagctttt atgcgttgga 20 <210> 610 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0360 Reverse <400> 610 ttcggcaacc ctagaagaga 20 <210> 611 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE052 Forward <400> 611 tctccaggct tttcctaacc aaga 24 <210> 612 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE052 Reverse <400> 612 tcaactggcc tcagtaaatt ctgc 24 <210> 613 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0045 Forward <400> 613 aagtagttga atctcgccgc 20 <210> 614 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0045 Reverse <400> 614 aggagcagat ggagatggtg 20 <210> 615 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0009 Forward <400> 615 gcaagaaatt gtcgttatgc aa 22 <210> 616 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0009 Reverse <400> 616 cccagtaaaa cttaaccgca ct 22 <210> 617 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1453 Forward <400> 617 ttctttttac tcaatttggt ttatgc 26 <210> 618 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1453 Reverse <400> 618 gacaaggttt cttggcctga 20 <210> 619 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2907 Forward <400> 619 atcaacggat ctggaggatc t 21 <210> 620 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2907 Reverse <400> 620 cacgttcatt tcgtgatgat g 21 <210> 621 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0064 Forward <400> 621 ttctgctgaa gatgctgcac 20 <210> 622 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0064 Reverse <400> 622 ccactgaagg actcaacggt 20 <210> 623 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0200 Forward <400> 623 actggagctt ctcctcctcc 20 <210> 624 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0200 Reverse <400> 624 gaggaaccaa caaaagctgc 20 <210> 625 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6392 Forward <400> 625 cccaaaagcc cttatcagtg t 21 <210> 626 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6392 Reverse <400> 626 tcgtttagct atggcgaaga a 21 <210> 627 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0817 Forward <400> 627 ggcactttga ctgttgcaga 20 <210> 628 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0817 Reverse <400> 628 tctcaatctt ggccagtcct 20 <210> 629 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE095 Forward <400> 629 tgcttaaacc cacttgcgtc ttg 23 <210> 630 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE095 Reverse <400> 630 tctgcacagc acaagacatt cg 22 <210> 631 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3536 Forward <400> 631 aatgccgtaa aatcgtcttc t 21 <210> 632 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3536 Reverse <400> 632 agattttgct ttgccctttt c 21 <210> 633 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1438 Forward <400> 633 gggggagatg ataacggatt 20 <210> 634 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1438 Reverse <400> 634 caaaattcca tcccctaccc 20 <210> 635 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3517 Forward <400> 635 cggagaagat ggagggagat a 21 <210> 636 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3517 Reverse <400> 636 gaaggaacag ctaccaaacc c 21 <210> 637 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2580 Forward <400> 637 tgctaacaac aatcaccacc a 21 <210> 638 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2580 Reverse <400> 638 ttaacccgat cacaaacttc g 21 <210> 639 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> CAMS-806 Forward <400> 639 tgtcacaagt gtcaaggtag gag 23 <210> 640 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-806 Reverse <400> 640 ccccaaaaat tttccctcat 20 <210> 641 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5357 Forward <400> 641 ttctgaagaa tcagattttg gat 23 <210> 642 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5357 Reverse <400> 642 tcattcatgt gacaatgttg ga 22 <210> 643 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5522 Forward <400> 643 aatgcaagca cagacacaca c 21 <210> 644 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5522 Reverse <400> 644 tggagacatt ctggttcatc c 21 <210> 645 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1464 Forward <400> 645 cacattgcca tgaaaatcca 20 <210> 646 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1464 Reverse <400> 646 gagcacttga aaacacaagc a 21 <210> 647 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6035 Forward <400> 647 tctttattgg taggataatc cacttg 26 <210> 648 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6035 Reverse <400> 648 acattcaagt ccagccatca g 21 <210> 649 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5872 Forward <400> 649 ttgtgtggtt agtggggtga t 21 <210> 650 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5872 Reverse <400> 650 acttggtggg aattcactgg 20 <210> 651 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0865 Forward <400> 651 tgtggtatct cgtgccttga 20 <210> 652 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0865 Reverse <400> 652 tttgaaggat ttgacacgca 20 <210> 653 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6264 Forward <400> 653 acatgctaag ccttgttccc t 21 <210> 654 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6264 Reverse <400> 654 attcgatggc ttaggcgtta t 21 <210> 655 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5377 Forward <400> 655 tattggaaac cgcactattg g 21 <210> 656 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5377 Reverse <400> 656 aatctcaccc tctggtttgg t 21 <210> 657 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPACS75 Forward <400> 657 taacaaacat atggtgagca tgaa 24 <210> 658 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPACS75 Reverse <400> 658 gtaggcggct agaggttagg t 21 <210> 659 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3743 Forward <400> 659 gggtggtgag attgaggaaa t 21 <210> 660 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3743 Reverse <400> 660 tccacctaag acgaggaact g 21 <210> 661 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3220 Forward <400> 661 tgcgaaaata cagtgggtag g 21 <210> 662 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3220 Reverse <400> 662 ccatatcagt tttcgccatg t 21 <210> 663 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5288 Forward <400> 663 ttgatccacg tgctaacttt tg 22 <210> 664 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5288 Reverse <400> 664 ccaatataca tacgcgttgg g 21 <210> 665 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1222 Forward <400> 665 gccattctcc ctctctttca 20 <210> 666 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1222 Reverse <400> 666 ttgaagggtc acaaacagaa tc 22 <210> 667 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1565 Forward <400> 667 gcccaacgtg tctatcgaat 20 <210> 668 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1565 Reverse <400> 668 gttttcgagt ttttggctcg 20 <210> 669 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3250 Forward <400> 669 tcctccaaaa acacacacag a 21 <210> 670 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3250 Reverse <400> 670 gaaagactgg tggtgggtgt a 21 <210> 671 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0880 Forward <400> 671 atacccccac cactagtctc c 21 <210> 672 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0880 Reverse <400> 672 gaaacaggga aaacatgcag a 21 <210> 673 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0448 Forward <400> 673 ccgaaaacac accaagatca 20 <210> 674 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0448 Reverse <400> 674 acagggaaaa catgcagacc 20 <210> 675 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2227 Forward <400> 675 aattatcaca acccatcatt tctc 24 <210> 676 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2227 Reverse <400> 676 ctggaccaaa atggaagaac a 21 <210> 677 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0517 Forward <400> 677 taatagaatg ggtgaggccg 20 <210> 678 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0517 Reverse <400> 678 cgaccatgtg tggttgattt 20 <210> 679 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3901 Forward <400> 679 ccacaacatc aaaaatggga c 21 <210> 680 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3901 Reverse <400> 680 agccccaggt tcattacatt c 21 <210> 681 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6053 Forward <400> 681 gcgtttgtag tccaacggtt a 21 <210> 682 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6053 Reverse <400> 682 gggatagata tgaccgggaa a 21 <210> 683 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0759 Forward <400> 683 agggacaata tgccaaaact t 21 <210> 684 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0759 Reverse <400> 684 gaatggcgtc ctcttactgc 20 <210> 685 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0136 Forward <400> 685 gtgaaccacg agcggattat 20 <210> 686 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0136 Reverse <400> 686 ctgcaacagt tgatcatcag aa 22 <210> 687 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2866 Forward <400> 687 ggcgtcctct tactgcctaa t 21 <210> 688 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2866 Reverse <400> 688 agggacaata tgccaaaact t 21 <210> 689 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4515 Forward <400> 689 aaagctctcc tccattgctt c 21 <210> 690 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4515 Reverse <400> 690 tggttgtgct gcattattca a 21 <210> 691 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2643 Forward <400> 691 cgtacgaaag ttccccaatc t 21 <210> 692 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2643 Reverse <400> 692 atttcaattg cttgtggatg g 21 <210> 693 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5085 Forward <400> 693 ttggctcgat tttcatttca c 21 <210> 694 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5085 Reverse <400> 694 tccttgaagt ggctaaggga t 21 <210> 695 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3524 Forward <400> 695 ttcctcttcc tttgcaacta gc 22 <210> 696 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3524 Reverse <400> 696 cagacgggag ccataaacat a 21 <210> 697 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0174 Forward <400> 697 cagccataag ctcccaagag 20 <210> 698 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0174 Reverse <400> 698 caccttctac tcccccaaca 20 <210> 699 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1942 Forward <400> 699 tagcagcctg gcttatgaga a 21 <210> 700 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1942 Reverse <400> 700 aataagcagt tacattaata ttggca 26 <210> 701 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2163 Forward <400> 701 ttgccaaggg taataagcag tt 22 <210> 702 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2163 Reverse <400> 702 gcctgggtta tgagaaggaa g 21 <210> 703 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0280 Forward <400> 703 agcagcctgg cttatgagaa 20 <210> 704 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0280 Reverse <400> 704 ttgccaaggg taataagcag tt 22 <210> 705 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-301 Forward <400> 705 ctgtccatgc ttgtgatgct 20 <210> 706 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CAMS-301 Reverse <400> 706 tgatttgtgc ctcgtttgag 20 <210> 707 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0783 Forward <400> 707 ttcgtcatcc atttttctaa caac 24 <210> 708 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0783 Reverse <400> 708 ttacatcctt tgccactgga c 21 <210> 709 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3409 Forward <400> 709 gcccaaccat attcacataa aaa 23 <210> 710 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3409 Reverse <400> 710 tgaggaataa gtcggaaggg t 21 <210> 711 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2077 Forward <400> 711 ggggattcac ttgtccctaa a 21 <210> 712 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2077 Reverse <400> 712 gggacgtaca aacaaatagc g 21 <210> 713 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0167 Forward <400> 713 gtgagtgcat gcaacacttg 20 <210> 714 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0167 Reverse <400> 714 ttttaaggca tctgttgggc 20 <210> 715 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CAMS-826 Forward <400> 715 cttgatctca agaaccagct acaa 24 <210> 716 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> CAMS-826 Reverse <400> 716 tgtacattga agacacggaa gaa 23 <210> 717 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4833 Forward <400> 717 aaccaaacct tgcaagctac a 21 <210> 718 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4833 Reverse <400> 718 ctggttgaac gagagtcttg g 21 <210> 719 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4805 Forward <400> 719 tctgcaattg ttctttgcct t 21 <210> 720 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4805 Reverse <400> 720 tatcatggtt ttgcagcctt c 21 <210> 721 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0265 Forward <400> 721 acatgatcaa tcagcagcca 20 <210> 722 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0265 Reverse <400> 722 tgcactcacc ctcttcacag 20 <210> 723 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5460 Forward <400> 723 ctttcaaccg ttgccataaa t 21 <210> 724 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5460 Reverse <400> 724 gagaaagaaa gggggtcttg a 21 <210> 725 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6682 Forward <400> 725 tgcactttcc aaatttgtgg t 21 <210> 726 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6682 Reverse <400> 726 aatccgaaaa agccgactaa a 21 <210> 727 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1543 Forward <400> 727 ctcctgttac ccccatttca 20 <210> 728 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1543 Reverse <400> 728 ggtgtaaagc caactccagc 20 <210> 729 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0585 Forward <400> 729 attggtttgg gagacacagc 20 <210> 730 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0585 Reverse <400> 730 aagccgaagg tgagcataga 20 <210> 731 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1104 Forward <400> 731 aagccgaagg tgagcataga t 21 <210> 732 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1104 Reverse <400> 732 gaggacgagg aagtggagag t 21 <210> 733 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1191 Forward <400> 733 gaggacgagg aagtggagag t 21 <210> 734 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1191 Reverse <400> 734 aagccgaagg tgagcataga t 21 <210> 735 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5056 Forward <400> 735 taccatccgt tgctcgagat 20 <210> 736 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5056 Reverse <400> 736 tgttgtcgac atgaatttct ca 22 <210> 737 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0288 Forward <400> 737 ccactcccag tggttgaaat 20 <210> 738 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0288 Reverse <400> 738 tttctgctca cgtttgcatt 20 <210> 739 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2531 Forward <400> 739 ccggtgttta taacgagcaa a 21 <210> 740 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2531 Reverse <400> 740 tgtgagggga aatcaatttt gt 22 <210> 741 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0371 Forward <400> 741 cagcaacgac aacctcatca 20 <210> 742 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0371 Reverse <400> 742 gcaaccaaaa gatgaattac cc 22 <210> 743 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1717 Forward <400> 743 acacatggtc atgcccctac 20 <210> 744 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1717 Reverse <400> 744 gccaaattaa tgacaaagca a 21 <210> 745 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0753 Forward <400> 745 gccttggtca aggtagtggt 20 <210> 746 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0753 Reverse <400> 746 gcaacaatcg tcctacatcc a 21 <210> 747 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1639 Forward <400> 747 tattgttagc gggggtgaac 20 <210> 748 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1639 Reverse <400> 748 ggttccatat agcccgtgtg 20 <210> 749 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1676 Forward <400> 749 ctgtgcatag cgggaactta g 21 <210> 750 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1676 Reverse <400> 750 atgatcaaat gtccatctcg g 21 <210> 751 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4783 Forward <400> 751 agaggcaaaa ggtttcgaga g 21 <210> 752 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4783 Reverse <400> 752 ctcaggcttg tgtttgctct t 21 <210> 753 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> MS3(AluI) Forward <400> 753 ccaccctaag atggttctag gata 24 <210> 754 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> MS3(AluI) Reverse <400> 754 gtttccatag agtagttgtg catg 24 <210> 755 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1454 Forward <400> 755 gaaagggttc tcgaggaaat g 21 <210> 756 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1454 Reverse <400> 756 atcaatccca aaaccatgtg a 21 <210> 757 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1620 Forward <400> 757 gatttgattc tgcgtccaaa a 21 <210> 758 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1620 Reverse <400> 758 tgaagggata tcaaaccagc a 21 <210> 759 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6343 Forward <400> 759 ttggggaaaa tgttggaatt t 21 <210> 760 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6343 Reverse <400> 760 caacctttct acctcccaag g 21 <210> 761 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1064 Forward <400> 761 gaagcttcca tttcagaggc t 21 <210> 762 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1064 Reverse <400> 762 caacttccga caacggatct a 21 <210> 763 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0538 Forward <400> 763 gtcatcagct gcaaagacca 20 <210> 764 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0538 Reverse <400> 764 acaacgtcga cctccaaaac 20 <210> 765 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE019 Forward <400> 765 aagtcatcag ctgcaaagac ca 22 <210> 766 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE019 Reverse <400> 766 ttcaacatgc atccagcttc tt 22 <210> 767 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2029 Forward <400> 767 caagtataat aaacaataat cccgtct 27 <210> 768 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2029 Reverse <400> 768 ttcagctcag gaagcaacat t 21 <210> 769 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0330 Forward <400> 769 ttgaatcgtt gaagcccatt 20 <210> 770 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0330 Reverse <400> 770 atctgaagct gggctccttt 20 <210> 771 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1609 Forward <400> 771 tctatctcac caaaacccac g 21 <210> 772 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1609 Reverse <400> 772 ctatctgaag ctgggctcct t 21 <210> 773 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0753 Forward <400> 773 atggtggact tcggaacaag 20 <210> 774 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0753 Reverse <400> 774 accgtctgaa gggctattcc 20 <210> 775 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5632 Forward <400> 775 tgggatacaa ctaaaacgca ctt 23 <210> 776 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5632 Reverse <400> 776 caagccagaa aattgaaatg g 21 <210> 777 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2460 Forward <400> 777 ttggaagtct tgttccgact 20 <210> 778 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2460 Reverse <400> 778 ttggagccta catccaaacc 20 <210> 779 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1454 Forward <400> 779 ttgattttgg aggcgaattt 20 <210> 780 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1454 Reverse <400> 780 ctctcctctg tttcgcgagt 20 <210> 781 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-864 Forward <400> 781 ctgttgtgga agaagaggac a 21 <210> 782 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> CAMS-864 Reverse <400> 782 gcttcttttt caacctcctc ct 22 <210> 783 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4865 Forward <400> 783 ccaaaagcaa gtggtcttat ca 22 <210> 784 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4865 Reverse <400> 784 acaccgcaaa ttacgaaaat g 21 <210> 785 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0325 Forward <400> 785 ggtaatggtt gtgtggggat 20 <210> 786 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0325 Reverse <400> 786 tcgtctcaaa ttacccatct ca 22 <210> 787 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4580 Forward <400> 787 cgatatagag gtgcggttga a 21 <210> 788 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4580 Reverse <400> 788 cctctgcaat tgaagttcac aa 22 <210> 789 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0939 Forward <400> 789 cttccaagtc cacccagaaa 20 <210> 790 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0939 Reverse <400> 790 tgggtggtta caaatcatct ca 22 <210> 791 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2548 Forward <400> 791 ccgtattttg ttacggcttc a 21 <210> 792 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2548 Reverse <400> 792 cttgaatttg atttgcctgg a 21 <210> 793 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0224 Forward <400> 793 gggctgaaaa atccatgaaa 20 <210> 794 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0224 Reverse <400> 794 tccgagaatt taaactacaa gatga 25 <210> 795 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2939 Forward <400> 795 gcaagccaca ctagccatct 20 <210> 796 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2939 Reverse <400> 796 cgtgagaata attgggtttt ga 22 <210> 797 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0159 Forward <400> 797 ttgcaaagta gtggtgctgc 20 <210> 798 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0159 Reverse <400> 798 agagaaatct cgggaaaccc 20 <210> 799 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5350 Forward <400> 799 cttggcacga actttgactt c 21 <210> 800 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5350 Reverse <400> 800 tagaaatgat ggcacttcgg t 21 <210> 801 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0836 Forward <400> 801 atttgtccca attcttcagg c 21 <210> 802 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0836 Reverse <400> 802 gcatcccgtt atctcaaatc a 21 <210> 803 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6257 Forward <400> 803 aaaacttcaa ctggaggcca t 21 <210> 804 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6257 Reverse <400> 804 ggacactttg ttgtttatcc ttcc 24 <210> 805 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2877 Forward <400> 805 ggcttgccat ttacattgtt g 21 <210> 806 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2877 Reverse <400> 806 ttccaagcaa caaagcacac 20 <210> 807 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CAMS-679 Forward <400> 807 tttgcatgtt ttacccattc c 21 <210> 808 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> CAMS-679 Reverse <400> 808 atgtgaaaca cataggtagc actga 25 <210> 809 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> HpmsE025 Forward <400> 809 tgagcatccc gttatctcaa atca 24 <210> 810 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE025 Reverse <400> 810 cccaattctt caggcaatct cc 22 <210> 811 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0031 Forward <400> 811 agcctggctc tagattgctt 20 <210> 812 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0031 Reverse <400> 812 ccatgatatc tctgtcccac c 21 <210> 813 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5645 Forward <400> 813 catgtactcc ccatgacacc t 21 <210> 814 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5645 Reverse <400> 814 acaattgcaa aaatgagacc g 21 <210> 815 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1018 Forward <400> 815 ggtggttaaa tctggggaaa a 21 <210> 816 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1018 Reverse <400> 816 acagcctctc tacttcctcg g 21 <210> 817 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3014 Forward <400> 817 ttgggggcta tcctgtactc 20 <210> 818 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3014 Reverse <400> 818 tgttaacctt ccagggttcg 20 <210> 819 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5323 Forward <400> 819 atcaagcctc cacaacaaca a 21 <210> 820 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5323 Reverse <400> 820 ctgttggagg tattggagct g 21 <210> 821 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0145 Forward <400> 821 tcgaacgttc ttgccaattt 20 <210> 822 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0145 Reverse <400> 822 tcagaaaccc taacatcttc acc 23 <210> 823 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0619 Forward <400> 823 gccctgaatc gtcctatttg 20 <210> 824 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0619 Reverse <400> 824 ggagtatagg gaaataatgc taggg 25 <210> 825 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2455 Forward <400> 825 tgtggagatg aataaatcgg c 21 <210> 826 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2455 Reverse <400> 826 cacccattag aaatagaaag aaatca 26 <210> 827 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1366 Forward <400> 827 gcgctatatg tcacaatccg t 21 <210> 828 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1366 Reverse <400> 828 aaatgaggaa aagataggga gga 23 <210> 829 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4714 Forward <400> 829 gccattttct aggccaaact c 21 <210> 830 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4714 Reverse <400> 830 gcgctatatg tcacaatccg t 21 <210> 831 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2632 Forward <400> 831 tttacggcta cgactacggt g 21 <210> 832 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2632 Reverse <400> 832 ggaggtctca cttttcggat t 21 <210> 833 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0433 Forward <400> 833 atctctgttg tgttgttgaa 20 <210> 834 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0433 Reverse <400> 834 gattgcgcta acccatgaat 20 <210> 835 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3023 Forward <400> 835 ttgctgtaag aaagtgcagc at 22 <210> 836 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3023 Reverse <400> 836 aacatcgcga ggatattgat g 21 <210> 837 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> BS2(SCAR) Forward <400> 837 gtcgacagat gtctagttct cg 22 <210> 838 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> BS2(SCAR) Reverse <400> 838 ggtacatcga aattcccata gctgaac 27 <210> 839 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0014 Forward <400> 839 aagtgttagt cggggaggtt g 21 <210> 840 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0014 Reverse <400> 840 attatgtgca gccaagattg tg 22 <210> 841 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2360 Forward <400> 841 ttgagacatt ctagtattgt gaaaaca 27 <210> 842 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2360 Reverse <400> 842 ttaaacctgc tttttgaggc a 21 <210> 843 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3420 Forward <400> 843 aagcgctttt taacgctttt c 21 <210> 844 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3420 Reverse <400> 844 ccctccattg ttagcttctt g 21 <210> 845 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5491 Forward <400> 845 tcctgaagcg ctttttaacg 20 <210> 846 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5491 Reverse <400> 846 cgggtgctgt aacatttctg 20 <210> 847 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5588 Forward <400> 847 gcaactctgt gaaatgggct 20 <210> 848 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5588 Reverse <400> 848 gagctggatc caacagcttc 20 <210> 849 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2330 Forward <400> 849 tttacttgga ttcaactctt ttatga 26 <210> 850 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2330 Reverse <400> 850 tatcatcaca ttccgcagca 20 <210> 851 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1152 Forward <400> 851 caaaggttgg ttccgatgtt 20 <210> 852 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1152 Reverse <400> 852 ttgaaagcat ttgacagggt aa 22 <210> 853 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0756 Forward <400> 853 tgcttctaag aaaccccaca a 21 <210> 854 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0756 Reverse <400> 854 aaaggggttg ggattgagat 20 <210> 855 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0895 Forward <400> 855 acgtgctggt ccttcttctg 20 <210> 856 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0895 Reverse <400> 856 taacgacaga agttgccggt 20 <210> 857 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0894 Forward <400> 857 attggagttt ggcaaaaacg 20 <210> 858 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0894 Reverse <400> 858 gccggtcgct atttcattta 20 <210> 859 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0892 Forward <400> 859 tgttgcagga agagaagggt 20 <210> 860 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0892 Reverse <400> 860 ttttctcagc ttggcatgaa 20 <210> 861 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1596 Forward <400> 861 ccaaacccca attacccttt 20 <210> 862 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1596 Reverse <400> 862 tgcatcaatg cttgttgttg t 21 <210> 863 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5163 Forward <400> 863 aatgagaagc cattgtggtt g 21 <210> 864 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5163 Reverse <400> 864 gagcagaagt cgatggagat g 21 <210> 865 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4095 Forward <400> 865 aaaaacgtgc tggtccttct t 21 <210> 866 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4095 Reverse <400> 866 gtttaacgac agaagttgcc g 21 <210> 867 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3475 Forward <400> 867 cagaagaagc agcaagagca t 21 <210> 868 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3475 Reverse <400> 868 tttctcagct tggcatgaag t 21 <210> 869 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPTr053(PvuII) Forward <400> 869 ccaaccctat tgaacgtctt 20 <210> 870 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPTr053(PvuII) Reverse <400> 870 ctgattcttg atgcctcttg 20 <210> 871 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPSSR9 Forward <400> 871 caagcaccta caaatgcaaa at 22 <210> 872 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPSSR9 Reverse <400> 872 ccggatgaga aaacttgcta ct 22 <210> 873 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0933 Forward <400> 873 gggttcaata aagctgtggg 20 <210> 874 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0933 Reverse <400> 874 tcgatgaaaa gtccttcaga aa 22 <210> 875 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0003 Forward <400> 875 ttgtcagtta gtaatggatc aagc 24 <210> 876 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0003 Reverse <400> 876 ccaaaagtca ttaccccaat aa 22 <210> 877 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1025 Forward <400> 877 ggtttcaatt ttctgctcag g 21 <210> 878 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1025 Reverse <400> 878 agaaaacgac caagaaacgg t 21 <210> 879 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0997 Forward <400> 879 tgtcacaatc cgtcgtgaat 20 <210> 880 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0997 Reverse <400> 880 tgccattttc taggccaaac 20 <210> 881 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0494 Forward <400> 881 cggcaaagtc gtacttggat 20 <210> 882 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0494 Reverse <400> 882 cagagcagga tgatttgcag 20 <210> 883 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6208 Forward <400> 883 aaacgaagca acatagcctg a 21 <210> 884 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6208 Reverse <400> 884 gcaaggatgg gttagttttc c 21 <210> 885 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3699 Forward <400> 885 tcgtggttac actggtgcat 20 <210> 886 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3699 Reverse <400> 886 accgggggtc tatcagaaac 20 <210> 887 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Hpms2~24 Forward <400> 887 tcgtattggc ttgtgattta ccg 23 <210> 888 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Hpms2?24 Reverse <400> 888 ttgaatcgaa tacccgcagg ag 22 <210> 889 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5383 Forward <400> 889 cacaaagggg tcaaaacact c 21 <210> 890 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5383 Reverse <400> 890 gagaaggatg acaatgggtg a 21 <210> 891 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6111 Forward <400> 891 tgtatcagga ggtaccgttg g 21 <210> 892 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6111 Reverse <400> 892 acgagagaga gagggtaggg a 21 <210> 893 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE096 Forward <400> 893 cgggtcaaac aaaaaccgaa gt 22 <210> 894 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE096 Reverse <400> 894 gcttgtggtt gagctcgctc tt 22 <210> 895 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1266 Forward <400> 895 cacgtacgac agattgatgg a 21 <210> 896 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1266 Reverse <400> 896 gacgacggaa actcttccac 20 <210> 897 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0583 Forward <400> 897 gatggagcga ttgatttggt 20 <210> 898 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0583 Reverse <400> 898 cgttcgaaaa tagagacttc caa 23 <210> 899 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2026 Forward <400> 899 gcagaatcat aagacaaaaa ggaaa 25 <210> 900 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2026 Reverse <400> 900 tctggtgctt tggttgctac t 21 <210> 901 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2459 Forward <400> 901 ggcaaagatt cgttctcttc a 21 <210> 902 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2459 Reverse <400> 902 atcccacaat ccaatcacaa a 21 <210> 903 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2978 Forward <400> 903 cattctgagt acagccaaca ca 22 <210> 904 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2978 Reverse <400> 904 gctctgtttt cctgcctttc t 21 <210> 905 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0378 Forward <400> 905 cataggccat gcatttcaga 20 <210> 906 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0378 Reverse <400> 906 attggaagca atgttgggat 20 <210> 907 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0377 Forward <400> 907 cagtctttca agaactagag agagaaa 27 <210> 908 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0377 Reverse <400> 908 ggagcaaaca cagcagaaca 20 <210> 909 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4481 Forward <400> 909 gcagtctttc aagaactaga gagagaa 27 <210> 910 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4481 Reverse <400> 910 aaagggcaac aagaatggag t 21 <210> 911 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3620 Forward <400> 911 tgaagttgaa aatgggctct g 21 <210> 912 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3620 Reverse <400> 912 tgcttttgct gctttcattt t 21 <210> 913 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3175 Forward <400> 913 ttcaaaagct cgctacacga t 21 <210> 914 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3175 Reverse <400> 914 gagcttggtt ggatcgaatt t 21 <210> 915 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0369 Forward <400> 915 cactcacgag aacgccatta 20 <210> 916 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0369 Reverse <400> 916 cggaaaactc gaagctcaac 20 <210> 917 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrBN1334 Forward <400> 917 tccaacatcg aagaagaaac aa 22 <210> 918 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrBN1334 Reverse <400> 918 aaccagaccc ctaaatttgg taa 23 <210> 919 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0772 Forward <400> 919 tctgcacata tcggagcaag 20 <210> 920 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0772 Reverse <400> 920 cccggtattt ttactatgtt tgc 23 <210> 921 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2814 Forward <400> 921 ggcctctgta taacaattca acg 23 <210> 922 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2814 Reverse <400> 922 tgcttttaac ttgcctgagg a 21 <210> 923 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0092 Forward <400> 923 aatcaaatgc catccaaagc 20 <210> 924 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0092 Reverse <400> 924 ttaccctccc tagaccccac 20 <210> 925 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0472 Forward <400> 925 tattccctat gggtgccgta 20 <210> 926 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0472 Reverse <400> 926 ttctcaaatt tattgcgttt agg 23 <210> 927 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0079 Forward <400> 927 tgggtccaat gcagtatcac tctt 24 <210> 928 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0079 Reverse <400> 928 cccttctttt tcttcaaatt ctatgtca 28 <210> 929 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5673 Forward <400> 929 gggacggaca aagaagaaaa c 21 <210> 930 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5673 Reverse <400> 930 atcaacataa gcaccaaggc a 21 <210> 931 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2092 Forward <400> 931 catgcaaatt tttcaccaaa 20 <210> 932 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB2092 Reverse <400> 932 ttttgagttc tttcccaatt cc 22 <210> 933 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0531 Forward <400> 933 tttcttgaac tttcctcctt ttt 23 <210> 934 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0531 Reverse <400> 934 aacccaacca tctctaccac c 21 <210> 935 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1601 Forward <400> 935 gtagctccat cgccagtttc 20 <210> 936 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1601 Reverse <400> 936 tccatcgtta aaacatccca a 21 <210> 937 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1479 Forward <400> 937 tttgggagag agaaagtgaa gaa 23 <210> 938 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1479 Reverse <400> 938 caattgcctt ctggagatcg 20 <210> 939 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3158 Forward <400> 939 ctctccgttt tccatccaaa t 21 <210> 940 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3158 Reverse <400> 940 aggttcatac cagtctccgc t 21 <210> 941 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0264 Forward <400> 941 caaacgaccc ttcagggata 20 <210> 942 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0264 Reverse <400> 942 caagaaagtg tgccccaaat 20 <210> 943 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0407 Forward <400> 943 catcaccttc catacaaatt tcc 23 <210> 944 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0407 Reverse <400> 944 cttggtgcac ctccatacct 20 <210> 945 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2075 Forward <400> 945 acgaggagaa aatcagcctc a 21 <210> 946 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2075 Reverse <400> 946 tttcgacctc acttcaccag t 21 <210> 947 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1672 Forward <400> 947 tttgttctct tcttcttctt tgtga 25 <210> 948 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1672 Reverse <400> 948 ccatgctgct gctgatctaa 20 <210> 949 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4151 Forward <400> 949 ctctcctcca ttttcaactg c 21 <210> 950 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4151 Reverse <400> 950 aaaaaggggt tgattgagct a 21 <210> 951 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0368 Forward <400> 951 attgcattaa aaccagcacc a 21 <210> 952 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0368 Reverse <400> 952 aacacacata acgtgtacac tcaca 25 <210> 953 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4115 Forward <400> 953 cgtaaaggag aagtaggaac aaca 24 <210> 954 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4115 Reverse <400> 954 gcaatttcag catcacaaag a 21 <210> 955 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5240 Forward <400> 955 tgcattcttc caatttccaa c 21 <210> 956 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5240 Reverse <400> 956 tgatacaacg agcagaggag c 21 <210> 957 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5408 Forward <400> 957 caaaatttgg ctcgttagca a 21 <210> 958 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5408 Reverse <400> 958 taagcatttt tgggggactt t 21 <210> 959 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1275 Forward <400> 959 tggaaaatgg tggtccatct a 21 <210> 960 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1275 Reverse <400> 960 tatgtaccaa atccccttcc c 21 <210> 961 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4373 Forward <400> 961 aagtcaagtc ccttttgcca t 21 <210> 962 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4373 Reverse <400> 962 acgagactcc aacgtgctct 20 <210> 963 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0615 Forward <400> 963 gccatttgaa agctggtcat 20 <210> 964 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0615 Reverse <400> 964 ccaaatccaa aagagaattg aaa 23 <210> 965 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> FPssrB4447 Forward <400> 965 tgaagtgttt gatattgttt ttgaa 25 <210> 966 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB4447 Reverse <400> 966 tcatgcattt aaaccattca ca 22 <210> 967 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0514 Forward <400> 967 tatgcggcct tttgattcat 20 <210> 968 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0514 Reverse <400> 968 ttccagtccc ttcaaacacc 20 <210> 969 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0883 Forward <400> 969 tgggacaaag tactgattct cg 22 <210> 970 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB0883 Reverse <400> 970 aatctcccga cgactcttca 20 <210> 971 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5607 Forward <400> 971 ggtggctatt taggggtgtg t 21 <210> 972 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5607 Reverse <400> 972 catccaagtt tggtgtgctt t 21 <210> 973 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1851 Forward <400> 973 tcaccaatgt gaccaaatcc 20 <210> 974 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1851 Reverse <400> 974 gttccgataa ccgaaagctg 20 <210> 975 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6036 Forward <400> 975 ttcaccccaa aggtagcatt a 21 <210> 976 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6036 Reverse <400> 976 ccataaggac ataaaaaccc ca 22 <210> 977 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5785 Forward <400> 977 tgcagtttga cctggaaaag t 21 <210> 978 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5785 Reverse <400> 978 tgtgctgtct tagccaaaag aa 22 <210> 979 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1054 Forward <400> 979 ggactgtcag tgggataagc a 21 <210> 980 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1054 Reverse <400> 980 tcccatggtt ttcttcagga 20 <210> 981 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1352 Forward <400> 981 tgaatttttg agccttatcc aa 22 <210> 982 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1352 Reverse <400> 982 tcaaactctt tttcttcact ttcc 24 <210> 983 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3355 Forward <400> 983 tttcggacca aagaatagtg aa 22 <210> 984 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3355 Reverse <400> 984 tcgattcaag agtcagcacc t 21 <210> 985 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4726 Forward <400> 985 gccttcatca acaaatttcc a 21 <210> 986 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4726 Reverse <400> 986 ctgagggctt ggattaggaa g 21 <210> 987 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4513 Forward <400> 987 atatgcggcc ttttgattca t 21 <210> 988 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4513 Reverse <400> 988 gacctggctc aagtgaacaa c 21 <210> 989 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3560 Forward <400> 989 cgcacacagc tctttgagac 20 <210> 990 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3560 Reverse <400> 990 ggcataaagt tgaggagacc a 21 <210> 991 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1036 Forward <400> 991 ttggcttata gtggccattt g 21 <210> 992 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1036 Reverse <400> 992 ggatcggaga agagatgaag g 21 <210> 993 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5782 Forward <400> 993 tcaagaaccc ccaagtaggt c 21 <210> 994 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5782 Reverse <400> 994 ccagagccca ctatgtgaga a 21 <210> 995 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0546 Forward <400> 995 aattcatcgt taatccatag tca 23 <210> 996 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0546 Reverse <400> 996 accccagcta gctaatgcaa 20 <210> 997 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1302 Forward <400> 997 tcaaaagtcc ttggttgttt ca 22 <210> 998 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB1302 Reverse <400> 998 caaacaagaa cagcgagcaa 20 <210> 999 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5372 Forward <400> 999 aacgcaccag acttttgaag a 21 <210> 1000 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5372 Reverse <400> 1000 aaggaagatg atgaggccaa t 21 <210> 1001 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2083 Forward <400> 1001 attctgtaca ctgcgccaaa g 21 <210> 1002 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2083 Reverse <400> 1002 atgctgcaat ctgcaatatc c 21 <210> 1003 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4020 Forward <400> 1003 taaatagacg aaaaggcgca a 21 <210> 1004 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4020 Reverse <400> 1004 tggcacttgt tgccataact a 21 <210> 1005 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3412 Forward <400> 1005 acagagtttg gcacttgttg c 21 <210> 1006 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3412 Reverse <400> 1006 tcggagtcaa gaaggagaac a 21 <210> 1007 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3345 Forward <400> 1007 gcgggctgga cagatattag 20 <210> 1008 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3345 Reverse <400> 1008 tcccgagctt aggagttgtg 20 <210> 1009 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2896 Forward <400> 1009 ctgtactgtc acaccccgac t 21 <210> 1010 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2896 Reverse <400> 1010 gtgccaaaat aaccagaacg a 21 <210> 1011 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0968 Forward <400> 1011 agcatcatca aagatgccaa g 21 <210> 1012 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0968 Reverse <400> 1012 cttgaagagg cttaattgcc a 21 <210> 1013 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1473 Forward <400> 1013 gcctagaggc cccaaataaa 20 <210> 1014 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1473 Reverse <400> 1014 aaacccttgg gcttctcatt 20 <210> 1015 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5715 Forward <400> 1015 tcacccaatg tctccactga 20 <210> 1016 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrN5715 Reverse <400> 1016 cccacaggaa gagagatgga 20 <210> 1017 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2534 Forward <400> 1017 ccaacacctt gattctttac ca 22 <210> 1018 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2534 Reverse <400> 1018 tcaatgttcc aactcagagc c 21 <210> 1019 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0774 Forward <400> 1019 tgcggtgtgc taaatagtgc 20 <210> 1020 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0774 Reverse <400> 1020 gctgttgcta ctcgcaatga 20 <210> 1021 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE075 Forward <400> 1021 gcggctcagc agaaagagag ag 22 <210> 1022 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE075 Reverse <400> 1022 tgccacagct ggagaacgta aa 22 <210> 1023 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1452 Forward <400> 1023 ctagataatc atggcggctc a 21 <210> 1024 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1452 Reverse <400> 1024 tggagaacgt aaagctacgg a 21 <210> 1025 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1436 Forward <400> 1025 ttcacctggt ggaggtagat aga 23 <210> 1026 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1436 Reverse <400> 1026 tcggtgtttg gatgttgcta 20 <210> 1027 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2782 Forward <400> 1027 cacacacgca catctctcat t 21 <210> 1028 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2782 Reverse <400> 1028 aatgcgacca acaagtaatc g 21 <210> 1029 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1653 Forward <400> 1029 acaacttgtt ccaccatcag g 21 <210> 1030 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1653 Reverse <400> 1030 cgtagggagg agcgatagag a 21 <210> 1031 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1651 Forward <400> 1031 tgttgaggaa aattgcaaag g 21 <210> 1032 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1651 Reverse <400> 1032 tttataaatt tgttttgggg gc 22 <210> 1033 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0326 Forward <400> 1033 attgcaaagg aatgggatca 20 <210> 1034 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0326 Reverse <400> 1034 tcttttataa atttgttttg gggg 24 <210> 1035 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1143 Forward <400> 1035 catggtttct gctgacgtgt 20 <210> 1036 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1143 Reverse <400> 1036 tccaagaaat accacaccca 20 <210> 1037 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3298 Forward <400> 1037 cacaacacaa aacccccata c 21 <210> 1038 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN3298 Reverse <400> 1038 aaactgttcg gattgttgac g 21 <210> 1039 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5339 Forward <400> 1039 acaaaggggg tttcaaatgt t 21 <210> 1040 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrB5339 Reverse <400> 1040 cgatgtgaaa ttgaattagt caga 24 <210> 1041 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2564 Forward <400> 1041 tttcttcttc tgggttcacc a 21 <210> 1042 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2564 Reverse <400> 1042 cctctctcgc tgtctctctc a 21 <210> 1043 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0300 Forward <400> 1043 ccttttacgc caaattccaa 20 <210> 1044 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0300 Reverse <400> 1044 aattaagctg gccacacagc 20 <210> 1045 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1054 Forward <400> 1045 attttgctcc tgggttttcc 20 <210> 1046 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1054 Reverse <400> 1046 cctcgtggag gtagagagac a 21 <210> 1047 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0251 Forward <400> 1047 aatattccga atctgcctca g 21 <210> 1048 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN0251 Reverse <400> 1048 cgctcagtaa tttcagcttc g 21 <210> 1049 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1531 Forward <400> 1049 atctttcatc cctttgtggc 20 <210> 1050 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1531 Reverse <400> 1050 ttcgcctctg tttcgattct 20 <210> 1051 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2784 Forward <400> 1051 ttttctcatc tttcatccct ttg 23 <210> 1052 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN2784 Reverse <400> 1052 ttcttcaggt gtggctgagt t 21 <210> 1053 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1974 Forward <400> 1053 aacctcatct gcacccttct t 21 <210> 1054 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1974 Reverse <400> 1054 agattttcat cagaactgcg g 21 <210> 1055 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0609 Forward <400> 1055 atggagatcg caacctcatc 20 <210> 1056 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0609 Reverse <400> 1056 gcggcaagaa gatgaaagtc 20 <210> 1057 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0720 Forward <400> 1057 gttgttgggt ggtacttggg 20 <210> 1058 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0720 Reverse <400> 1058 ggaagatctc aaatgggtcg 20 <210> 1059 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1514 Forward <400> 1059 atggcggttc tttttatcgt t 21 <210> 1060 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1514 Reverse <400> 1060 aggaagatct caaatgggtc g 21 <210> 1061 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1464 Forward <400> 1061 ttgtcgaaaa ttaggcgttc tt 22 <210> 1062 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1464 Reverse <400> 1062 tgggaaatgg ctaattcagg 20 <210> 1063 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4641 Forward <400> 1063 gcatctctgc tctttccgc 19 <210> 1064 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4641 Reverse <400> 1064 atcgaagaag aaagggggat t 21 <210> 1065 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0600 Forward <400> 1065 gccctcctca accctaaaaa 20 <210> 1066 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0600 Reverse <400> 1066 tgcgccatat tggttgaata 20 <210> 1067 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HpmsE108 Forward <400> 1067 ggccgcgtca accctaaaaa 20 <210> 1068 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE108 Reverse <400> 1068 tgggagggaa aagtaagcga aa 22 <210> 1069 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6151 Forward <400> 1069 aagaatgcgc cttcaatacc t 21 <210> 1070 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrB6151 Reverse <400> 1070 agaggaaaca acccttttcc a 21 <210> 1071 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3017 Forward <400> 1071 tccttcttcg tagggcatca 20 <210> 1072 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrB3017 Reverse <400> 1072 cctcctcgag cttgtcattt 20 <210> 1073 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1430 Forward <400> 1073 gccagcaaaa ctccaaagaa 20 <210> 1074 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC1430 Reverse <400> 1074 ccagcagcac caagagtaca 20 <210> 1075 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0886 Forward <400> 1075 ttcccaacaa aatcaaattg c 21 <210> 1076 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0886 Reverse <400> 1076 ttttgaaggg ttttggcaac 20 <210> 1077 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> HpmsE128 Forward <400> 1077 tggatcccaa aagactcaga aca 23 <210> 1078 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> HpmsE128 Reverse <400> 1078 tatttccctc agtcgaggtc gt 22 <210> 1079 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0246 Forward <400> 1079 ctcccaccag agtccttttg 20 <210> 1080 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0246 Reverse <400> 1080 cagaccggct tcgaaataaa 20 <210> 1081 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0647 Forward <400> 1081 gcttttctta aagttcttag tttccaa 27 <210> 1082 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0647 Reverse <400> 1082 tggtcatgtc gtttgcattt 20 <210> 1083 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0972 Forward <400> 1083 ccccctaatt cgacccttt 19 <210> 1084 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrC0972 Reverse <400> 1084 ttttggggtt ttgcaatcat 20 <210> 1085 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1250 Forward <400> 1085 ggcctctgtg ttttcctctc t 21 <210> 1086 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN1250 Reverse <400> 1086 ctcatttctt gcaaaacgag c 21 <210> 1087 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0538 Forward <400> 1087 ctggcctctg tgttttcctc 20 <210> 1088 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0538 Reverse <400> 1088 ccgtacagct gaatccccta 20 <210> 1089 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1854 Forward <400> 1089 tctggcctct gtgttttcct 20 <210> 1090 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1854 Reverse <400> 1090 ctgacttgca tctccatagc c 21 <210> 1091 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0050 Forward <400> 1091 cactgaaaat ttgcttttct ttga 24 <210> 1092 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW0050 Reverse <400> 1092 tcttgaccag cagtatccca 20 <210> 1093 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1621 Forward <400> 1093 tccaattgat gaacaagcat tc 22 <210> 1094 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1621 Reverse <400> 1094 tttgcaaatt tgaaggacag g 21 <210> 1095 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1296 Forward <400> 1095 tacaaaagga agccgaggtc 20 <210> 1096 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPssrW1296 Reverse <400> 1096 tctgtagccg gtggctctac 20 <210> 1097 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4798 Forward <400> 1097 caagagccat tactcttccc c 21 <210> 1098 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> FPssrN4798 Reverse <400> 1098 catcgtagcg cctttctaca c 21

Claims (14)

It is selected from DNA generated by amplifying genomic DNA of Capsicum annum with a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n. The average distance between adjacent loci of gene association group 1 of the F2 population capsicum anium plant obtained by self-pollination of F1 produced by crossing between the intraspecies population Capsicum annuum CM334 and capsicum annuum ECW123R High-density gene-associated mapping DNA of 5.45 cM (where n is one of the integers from 1 to 81, 5, 6, 19, 26, 39, 40, 49, 50, 61, and 71 are excluded) . delete A primer set for amplifying the marker DNA of claim 1, selected from among primer sets comprising a forward primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n-1 and a reverse primer composed of the nucleic acid sequence represented by SEQ ID NO: 2n ( At this time, the n is an integer from 1 to 81, 5, 6, 19, 26, 39, 40, 49, 50, 61 and 71 are excluded). delete delete delete delete delete delete delete delete delete delete delete

Images