KR20090032260A - Method for manufacturing feed for a deer - Google Patents

Abstract

Deer feed using probiotics is provided to enable deer to improve immunity by delivering effects of the probiotics, thereby producing high quality Cervi Parvum Cornu. A production method for deer feed comprises the 35-45 parts of corn kernel, the 10-20 parts of perilla meal, the 78-80 parts of bean-curd dregs, the 10-20 parts of milo bran, the 10-20 parts of Sorghum, the 15-25 parts of ginseng dregs, the 25-35 parts of wormwood, the 8-12 parts of probiotics and the 2.5-3.5 parts of salt on a basis of the 30 parts of barley bran by weight. All the ingredients are aged at a temperature of 20~30°C for 20-40 days. The 11-13 kg of aged feed is supplied to deer a day. The probiotics contains Lactobacillus casei, Aspergillus Oryzae, Saccharomyces cerevisiae, Bacillus Subtilis and Streptomyces griseus.

Description

Method of manufacturing deer feed and deer feed produced by the same {Method for manufacturing feed for a deer}

The present invention relates to a method for preparing a deer feed and a deer feed produced by the same, more specifically, when preparing a feed provided to the deer, barley bran, corn grains, maize, bean curd, sorghum bran, soybean bran, ginseng The present invention relates to a method of preparing a deer feed prepared by adding cheese lactic acid bacilli, yeast mold, baker's yeast, Bacillus subtilis and actinomycetes obtained by culturing with gourd and salt, and a deer feed produced by the same.

The deer are distributed worldwide and are classified into about 16 genera, 46 species, and 150 to 200 subspecies. The size of the deer varies greatly, and there are also large deer that weigh about 1.5kg to over 800kg.

These deer have horns, and their growing horns are called antlers. Deer antlers are freshly grown non-osteophilized young horns of deer belonging to the family Deer and processed and dried. The deer's horns drop hard old horns and grow new horns in the spring. It is preferable to cut and harvest in about 90 days.

The antler contains all the nutrients required by the human body, such as proteins, lipids, amino acids, gliacosaminoglycans, growth hormones, and growth factors, and also contains trace elements and minerals. It has been recognized as a medicine of medicine in countries such as Japan and Japan.

Deer antler that has such effect depends on the medicinal antler's medicinal effect, and in order to obtain antler antler, it is necessary to develop a deer feed without antibiotics and provide it to the deer steadily. It is necessary.

An object of the present invention is to produce a feed provided to the deer, by directly culturing the probiotic having a variety of beneficial effects and then added to the feed, the deer antler intake of the feed is superior to the deer deer antler It is to provide a method for producing a deer feed and a deer feed produced thereby to have efficacy.

Method of producing a deer feed of the present invention for achieving the above object is based on 30 parts by weight of barley bran, here corn grains 35 to 45 parts by weight, 10 to 20 parts by weight of bakja, bean curds 70 to 80 parts by weight, sorghum bran 10 to 20 parts by weight, 10 to 20 parts by weight of soybean bran, 15 to 25 parts by weight of ginseng foil, 25 to 35 parts by weight of mugwort, 8 to 12 parts by weight of probiotics and 2.5 to 3.5 parts by weight of salt, It is characterized by aging for 20 to 40 days at a temperature.

Here, the probiotic includes Lactobacillus casei, yeast fungus (Aspergillus Oryzae), Saccaromyces cerevisiae, Bacillus Subtilis and Streptomyces griseus.

In addition, the present invention provides a deer feed prepared by the above method.

The deer feed according to the present invention is prepared by mixing the probiotic cultured directly with other materials, the efficacy of the probiotic is delivered to the deer helps the deer to produce antler with excellent efficacy, deer feed according to the present invention Does not contain antibiotics at all, strengthening deer's immunity has the effect of preventing disease easily.

Hereinafter, the present invention will be described in more detail.

Ingredients constituting the deer feed according to the present invention comprises plant nutrients such as barley bran, corn grains, maize, soybean meal, sorghum, soybean bran, ginseng gourd, wormwood and salt and probiotics. In addition, the ratio of the material is added based on 30 parts by weight of barley bran, here 35 to 45 parts by weight of corn grains, 10 to 20 parts by weight of bakja, bean curds 70 to 80 parts by weight, sorghum bran 10 to 20 parts by weight, 10-20 parts by weight of soybean bran, 15-25 parts by weight of ginseng, 25-35 parts by weight of mugwort, 8-12 parts by weight of probiotics and 2.5-3.5 parts by weight of salt.

At this time, the bean curd is preferably made of 85 to 95% by weight of the total amount of bean curd, the mugwort is preferably used after being harvested, dried and pulverized in powder form. In particular, after the wormwood is prepared by adding to the deer feed, when the feed is ingested by the deer, the efficacy of the wormwood is delivered to the horn of the deer can be obtained antler with the efficacy of the wormwood.

In addition, the probiotic is made of Lactobacillus casei, yeast fungus (Aspergillus Oryzae), Saccaromyces cerevisiae, Bacillus Subtilis and Streptomyces griseus, each of the above probiotics Efficacy and characteristics are as follows.

First, the cheese lactic acid bacillus prevents diarrhea, inhibits the production of ammonia and intestinal harmful bacteria and rot bacteria, prevents bacterial enteritis, and improves feed efficiency and weight ratio.

The yeast fungus improves the productivity of livestock, produces fibrinolytic enzymes to help the digestion of fibrin, promotes digestive absorption of feed nutrients, and removes high molecular and low molecular odors.

The baker's yeast improves the productivity of livestock, removes low molecular odors, induces decomposition of starch, and produces monosaccharides such as glucose.

The Bacillus subtilis increases intestinal beneficial bacteria, produces proteolytic enzymes, removes polymers, low molecular odors, and produces useful substances.

The actinomycetes produce digestive enzymes, effectively remove low molecular weight fatty acids that cause malodors, and have an antibiotic action that suppresses the growth of harmful bacteria.

Referring to the method of culturing each of the probiotics having the above characteristics and efficacy as follows.

< Cheese  Lactic acid bacilli ( Lactobacillus casei ) Culture>

1) Flatbed (Solid) stage

Put 200ml of distilled water and 12.4g of MRS Agar medium into the Erlenmeyer flask and stir for 15 minutes. After autoclaving at 121 ° C. for 15 minutes in an autoclave, it is immersed in 53 ° C. water and cooled down to 55 ° C. temperature. In a sterile chamber, 5 ml of the medium is dispensed in a petri dish to make a plate medium. The plate medium is inoculated with platinum of Lactobacillus casei with platinum and sealed with parafilm, followed by incubation for 24 hours in an incubator at 36 ° C.

2) spawning medium (liquid medium)

200 ml of distilled water and 11 g of MRS broth medium were added to an Erlenmeyer flask and stirred for 15 minutes. Make 10 pieces of 10 ml each in a falcon tube and autoclave at 121 ° C. for 15 minutes in an autoclave and cool. The liquid medium is inoculated with a colony of Lactobacillus casei cultured in the plate medium and incubated in a shaking incubator at 32 ° C. for 24 hours.

3) Enrichment medium (liquid medium) step

1 L of distilled water and 55 g of MRS broth medium were added to the flask and dissolved, followed by high temperature sterilization at 121 ° C. for 15 minutes in an autoclave. The medium is inoculated with Lactobacillus casei bacteria cultured in a liquid medium as a previous step and incubated in a fermenter at 32 ° C. for 24 hours.

<Yeast mold ( Aspergillus Oryzae ) Culture>

1) Flatbed (Solid) stage

Put 200ml of distilled water and 7.8g of PD Agar medium into the Erlenmeyer flask and stir for 15 minutes. After autoclaving at 121 ° C. for 15 minutes in an autoclave, it is immersed in 53 ° C. water and cooled down to 55 ° C. temperature. In a sterile chamber, 5 ml of the medium is dispensed in a petri dish to make a plate medium. The plate medium was inoculated with platinum of Aspergillus Oryzae bacteria with platinum, sealed with a para film, and then incubated in an incubator at 36 ° C. for 24 hours.

2) spawning medium (liquid medium)

Put 200ml of distilled water and 4.8g of PD Broth medium into the Erlenmeyer flask and stir for 15 minutes. Make 10 pieces of 10 ml each in a falcon tube and autoclave at 121 ° C. for 15 minutes in an autoclave and cool. The liquid medium was inoculated with a colony of Aspergillus Oryzae cultured in the plate medium and incubated in a shaking incubator at 32 ° C. for 48 hours.

3) Enrichment medium (liquid medium) step

1 L of distilled water and 24 g of PD Broth medium were dissolved in a flask, and then sterilized at a high temperature of 121 ° C. for 15 minutes in an autoclave. The medium is inoculated with Aspergillus Oryzae cultured in a liquid medium as a previous step and incubated in a fermenter at 32 ° C. for 24 hours.

<Bread yeast ( Saccaromyces cerevisiae ) Culture>

1) Flatbed (Solid) stage

Put 200ml of distilled water and 7.8g of PD Agar medium into the Erlenmeyer flask and stir for 15 minutes. After autoclaving at 121 ° C. for 15 minutes in an autoclave, it is immersed in 53 ° C. water and cooled down to 55 ° C. temperature. In a sterile chamber, 5 ml of the medium is dispensed in a petri dish to make a plate medium. The plate medium was inoculated with platinum of colony of Saccaromyces cerevisiae with platinum, sealed with parafilm, and incubated in an incubator at 36 ° C. for 24 hours.

2) spawning medium (liquid medium)

Put 200ml of distilled water and 4.8g of PD Broth medium into the Erlenmeyer flask and stir for 15 minutes. Make 10 pieces of 10 ml each in a falcon tube and autoclave at 121 ° C. for 15 minutes in an autoclave and cool. The liquid medium is inoculated with a colony of Saccaromyces cerevisiae cultured in the plate medium and incubated for 24 hours in a shaking incubator at 32 ° C.

3) Enrichment medium (liquid medium) step

1 L of distilled water and 24 g of PD Broth medium were dissolved in a flask, and then sterilized at a high temperature of 121 ° C. for 15 minutes in an autoclave. The medium is inoculated with Saccaromyces cerevisiae cultured in a liquid medium as a previous step and incubated in a shaking incubator at 32 ° C. for 24 hours.

<Bacillus subtilis ( Bacillus Subtilis ) Culture>

1) Flatbed (Solid) stage

Put 200ml of distilled water and 8g of LB Agar medium into the Erlenmeyer flask and stir for 15 minutes. After autoclaving at 121 ° C. for 15 minutes in an autoclave, it is immersed in 53 ° C. water and cooled down to 55 ° C. temperature. In a sterile chamber, 5 ml of the medium is dispensed in a petri dish to make a plate medium. The plate medium was inoculated with Bacillus subtilis bacteria colored platinum (colony) and sealed with a para film, and then incubated in an incubator at 36 ° C. for 24 hours.

2) spawning medium (liquid medium)

Put 200ml of distilled water and 5g of LB broth medium into the Erlenmeyer flask and stir for 15 minutes. Make 10 pieces of 10 ml each in a falcon tube and autoclave at 121 ° C. for 15 minutes in an autoclave and cool. The liquid medium is inoculated with a colony of Bacillus subtilis bacteria cultured in the plate medium and incubated in a shaking incubator at 32 ° C. for 24 hours.

3) Enrichment medium (liquid medium) step

1 L of distilled water and 25 g of LB broth medium were dissolved in a flask, and then sterilized at a high temperature of 121 ° C. for 15 minutes in an autoclave. The medium is inoculated with Bacillus subtilis bacteria cultured in a liquid medium as a previous step and incubated in a shaking incubator at 32 ° C. for 24 hours.

Actinomycetes Streptomyces griseus ) Culture>

1) Flatbed (Solid) stage

Put 200ml of distilled water and 29.2g of Bennett's Agar medium into the Erlenmeyer flask and stir for 15 minutes. After autoclaving at 121 ° C. for 15 minutes in an autoclave, it is immersed in 53 ° C. water and cooled down to 55 ° C. temperature. In a sterile chamber, 5 ml of the medium is dispensed in a petri dish to make a plate medium. The plate medium was inoculated with platinum of Streptomyces griseus bacteria with platinum and sealed with parafilm, and then incubated in an incubator at 36 ° C. for 24 hours.

At this time, the Bennett's agar medium is composed of glucose 10g, yeast extract 1g, peptone 2g, beef extract 1g, agar 15.2g, may replace the Bennett's agar medium 8g LB agar medium.

2) spawning medium (liquid medium)

Put 200ml of distilled water and 14g of Bennett's Broth medium into the Erlenmeyer flask and stir for 15 minutes. Make 10 pieces of 10 ml each in a falcon tube and autoclave at 121 ° C. for 15 minutes in an autoclave and cool. The liquid medium is inoculated with colony of Streptomyces griseus cultured in the plate medium and incubated in a shaking incubator at 32 ° C. for 24 hours.

At this time, the Bennett's broth medium consists of 10 g of glucose, 1 g of yeast extract, 2 g of peptone, and 1 g of beef extract, and 5 g of LB broth medium may be used as a substitute for Bennett's broth medium.

3) Enrichment medium (liquid medium) step

1 liter of distilled water and 70 g of Bennett's Broth medium were added to the flask and dissolved, followed by high temperature sterilization at 121 ° C. for 15 minutes in an autoclave. The medium is inoculated with Streptomyces griseus bacteria cultured in a liquid medium as a previous step and incubated in a shaking incubator at 32 ° C. for 24 hours.

At this time, the Bennett's broth medium consists of 50 g of glucose, 5 g of yeast extract, 10 g of peptone, 5 g of beef extract, and 25 g of LB broth medium may be used as a substitute for the Bennett's broth medium.

Each live cell cultured in this way is added in the same proportion and mixed with other materials in the preparation of the deer feed of the present invention.

The mixed material is aged for 20 to 40 days at a temperature of 20 ~ 30 ℃ is supplied to the deer, the amount of feed per day is preferably 11 ~ 13kg to a deer.

Claims (5)

Based on 30 parts by weight of barley bran, corn grains 35 to 45 parts by weight, 10 to 20 parts by weight of maize gourd, 70 to 80 parts by weight of bean curd, 10 to 20 parts by weight of sorghum bran, 10 to 20 parts by weight of soybean bran, ginseng foil Deer feed, characterized in that 15 to 25 parts by weight, mugwort 25 to 35 parts by weight, probiotics 8 to 12 parts by weight and salt 2.5 to 3.5 parts by weight, and aged for 20 to 40 days at a temperature of 20 to 30 ℃ Manufacturing method. The method of claim 1, Method for producing a deer feed, characterized in that the soybeans contain 85 to 95% by weight of water. The method of claim 1, The wormwood is a method of producing a deer feed, characterized in that the powder form prepared by grinding after drying. The method of claim 1, The probiotic is a method of producing a deer feed characterized in that it comprises cheese lactobacillus (Lactobacillus casei), yeast fungus (Aspergillus Oryzae), bread yeast (Saccaromyces cerevisiae), Bacillus subtilis and Streptomyces griseus. Deer feed, characterized in that produced by the method according to any one of claims 1 to 4.