KR20090002680A - Anti-wrinkle cosmetic composition - Google Patents

Abstract

A cosmetic composition for improving wrinkle, and a method for preparing the cosmetic material are provided to enhance the effect promoting the synthesis of collagen. A cosmetic composition for improving wrinkle contains the fermentation extract which is obtained by fermenting the mushroom fungi of at least one selected from Angelica gigas, Angelica dahurica, insam (ginseng), Cervus nippon, aeonia lactiflora and Glycyrrhiza glabra with yeast, lactobacillus and bifidus, and extracting it, as an active ingredient.

Description

Anti-wrinkle cosmetic composition

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly, to a cosmetic composition for improving wrinkles having excellent collagen synthesis promoting effect, safe for skin, and an excellent effect for improving skin wrinkles and wound healing and sustaining the effects thereof. .

Collagen, a major component of the extracellular matrix, is present in extracellular epilepsy as a major substrate protein produced by skin fibroblasts. It is also an important protein, accounting for about 30% of the total weight of biological proteins, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bones and skin (dermis). Most other sieve structures exist as fibrous inclusions.

Collagen is a relatively weak immunogen, partly due to the shielding of latent antigenic determinants by the collagen's helix, which also makes collagen resistant to proteolysis. The main functions of collagen are known as mechanical firmness of skin, reluctance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation (in organic growth or wound healing) (Van der Rest et al. , Ann NY Acad Sci, 1990).

This collagen is reduced by age and photoaging by UV irradiation, which is known to be closely associated with wrinkle formation of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and promotes the synthesis of collagen in the damaged epithelium, allowing the wound to recover quickly and without scars.

Conventionally, in order to take advantage of collagen's skin moisturizing effect and wound healing effect, products containing collagen in an external cosmetic composition such as cosmetics or ointment have been released. However, these products are collagen, which is a polymer material, applied to the skin surface. Because it is difficult to absorb the percutaneous effect of moisturizing or wound healing, it could not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, interest in collagen synthesis promoting substances has been increased, and known collagen synthesis promoting substances are vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. 35 al, Adv. Enzymol., 41, p.425, 1974), protein from animal placenta (Japanese Patent Laid-Open No. 8-231370), betulinic acid (Japanese Patent Laid-Open No. 8-208424), Chlorella extract (Japanese Patent Application Laid-open No. Hei 9-40526, Japanese Patent Application Laid-open No. Hei 10-36283, fibroblast proliferation promoting action) and the like.

However, these materials have limited usage due to safety problems such as irritation and redness during skin application, or the effects are insignificant, and thus the effect of improving skin function or wound healing could not be expected. Therefore, there is an urgent need for the development of a new skin external preparation composition that is safer and more effective in vivo than existing external skin preparation compositions.

On the other hand, fermentation is a process of decomposing organic matter using enzymes of microorganisms. Fermentation and decay are carried out by a similar process, but when decomposition produces a substance that is useful in our lives, it is called fermentation. Therefore, fermenting the herbal medicine using microorganisms reduces the toxicity of the herbal medicine, lowers the molecular weight, facilitates digestion, or facilitates transdermal absorption when applied to the skin, thereby improving bioavailability.

In addition, herbal medicines may exhibit pharmacological effects by themselves, but the pharmacological effects may be enhanced through fermentation, or harmful ingredients may be made by the metabolism of microorganisms, thereby exerting new pharmacological effects that were not found in herbal medicines before fermentation.

In order to solve the problems of the prior art as described above, an object of the present invention is to provide a cosmetic composition for wrinkle improvement excellent in the skin wrinkles improvement effect and wound healing effect and excellent durability of the collagen synthesis safe and excellent effect It is done.

Another object of the present invention is to further enhance the pharmacological effect of each medicine through the fermentation to provide a cosmetic composition for improving wrinkles improved collagen synthesis further.

In order to achieve the above object, the present invention

Wrinkles comprising fermented extract extracted by fermenting mushroom fungus fermented with at least one kind selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice as yeast, lactic acid bacteria or bifidus as an active ingredient It provides a cosmetic composition for improvement.

Hereinafter, the cosmetic composition for improving wrinkles of the present invention will be described in detail.

The cosmetic composition for improving wrinkles of the present invention is effective fermentation extract extracted by fermenting mushroom fungus fermented with a yeast, lactic acid bacteria or bifidus bacteria selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice. It is characterized by including as a component. The fermentation extract is a step of extracting mushroom fungi fermenting mushrooms fermenting the medicinal herbs selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice; b) fermenting the mushroom fermented product of step a) with yeast, lactic acid bacteria or bifidus bacteria; And c) separating the fermentation extract from the fermentation in step b).

Angelica gigas is a perennial root belonging to the genus Umbelliferae and is used for physical weakness, headache, constipation, and the like. The angelica dahurica is a biennial herb belonging to the family Umbelliferae, and is used for headaches, dizziness, and guanwasa. The ginseng is a perennial root of perilla belonging to the genus Ogapi (Araliaceae) is used for blood circulation, cold hands, bronchitis and the like. The antler (Cervus nippon) is dried by harvesting the camouflage of the plum or aquatic animal belonging to the family Deeraceae (Cervidae), protect the liver and gods and strengthen bones and muscles. The red peony (Paeonia lactiflora) is a perennial herb belonging to Ranunculus (Ranunculaceae) is used to promote blood circulation, pain relief, jingyeong. The licorice (Glycyrrhiza glabra) is a perennial herb belonging to the legume (Legumisosae), dried in the shade and used as medicinal or tea, and used for neuralgia, cough, arteriosclerosis, and difficulty urination.

The medicinal herb can be prepared as an extract according to a conventional method. For example, the extract can be extracted with an organic solvent such as distilled water, anhydrous C1-C4 or hydrous lower alcohol, concentrated under reduced pressure, and dried to obtain a primary extract. have. Specifically, one or more medicinal herbs selected from the group consisting of Angelica, White Paper, Ginseng, Deer Antler, Red Peony and Licorice, which are commercially available as herbal medicines, are pulverized finely, and 5 to 20 volumes of water or carbon number is used for the dry weight of the ground product. Organic extracts such as 1-4 anhydrous or hydrous lower alcohols are heated at 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser to extract the extracts of the medicinal herbs. After filtration with a filter cloth, the residue is further extracted one or more times in the same manner, the extracts are combined and concentrated under reduced pressure, lyophilized or spray-dried to obtain a dry extract.

The medicinal herbs selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice obtained as above are first fermented through mushrooms. The fungus is a mushroom of medicinal mushrooms, such as chaga (Inonnotus-obliquus), Cordyceps sinensis, Paecilomyces japonica , Trichoma matsutake, phellinus linteus, Ganoderma lucidum, etc. Can be used.

Preferably, the mushroom bacteria fermentation is inoculated at 1 to 10% by weight of the mushroom bacteria in each of the medicinal herbs or slices, powdered ground powder selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice. And, after fermentation for 60 days at 18 to 25 ℃, humidity 60 to 70% for 60 days to extract it can be obtained mushroom fermentation.

Extraction of the mushroom fungi fermentation is 50 to 100 ℃ in an extractor equipped with a reflux cooler with an organic solvent, such as 5 to 20 volumes of water or an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms based on the dry weight of the fermented product. Extract the extracts of the medicines by heating for ˜5 hours. Then, the filtrate was filtered with a filter cloth, and the residue was further extracted one or more times in the same manner, the extracts were combined under reduced pressure, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dry extract.

The mushroom bacteria fermentation is then subjected to sterilization for smooth fermentation by yeast, lactic acid bacteria or bifidus bacteria. The mushroom bacteria fermentation may be added to each yeast, lactic acid bacteria or bifidus bacteria and cultured to extract the respective fermentation extracts. Preferably, the mushroom fermentation product is preferably added in an amount of 1 to 20% by weight to a medium for culturing yeast, lactic acid bacteria or bifidus bacteria.

The yeast may be used Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii and the like.

Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis and the like can be used.

The bifidus bacteria may be Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis and the like.

Preferably, when using yeast as a microorganism, the yeast is inoculated in a basic medium (YM medium) each containing mushroom fermented product of a medicinal herb selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice. (Preferred concentration (about 10 ^{5-10 7} cells / mL), and then incubated for 30 to 120 hours while maintaining the conditions of pH ^5.5 to 6.5, temperature 25 to 35 ℃, aeration amount 0.05 to 0.5 VVM. In addition, the fermentation broth may be centrifuged to remove polymer precipitates and yeast cells, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective medicinal fermentation extracts.

When lactic acid bacteria are used as microorganisms, lactic acid bacteria are inoculated in a basic medium (MRS medium) containing fermented mushroom fungi of at least one kind selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice (preferred concentration). (About 10 ^{5-10 7} cells / mL), and incubate for 24 to 120 hours while maintaining the conditions of pH 6.5-7.5, temperature 35-38 ° C., and aeration rate 1 VVM. By removing the polymer precipitate and lactic acid bacteria, and concentrated under reduced pressure to 60 ℃ in a distillation apparatus equipped with a cooling capacitor and then lyophilized to obtain the respective herbal fermentation extract.

When using bifidus as a microorganism, inoculate bifidus into basic medium (BL medium) containing fermented mushroom fungi of at least one kind selected from the group consisting of Angelica, white paper, ginseng, antler, red pea and licorice. After the desired concentration (about 10 ^{5-10 7} cells / mL), the culture is incubated for 24 to 120 hours while maintaining the pH 6.5-7.5, temperature 35-38 ° C., anaerobic culture conditions, and then the fermentation broth is centrifuged. The polymer precipitate and the bifidus cells were removed, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then lyophilized to obtain respective medicinal herb fermentation extracts.

In addition, it is preferable that the total amount of the fermentation extract of the medicinal herb in the cosmetic composition for wrinkle improvement of the present invention is included in 0.0001 to 20% by weight, more preferably 0.01 to 10% by weight. If the content is less than 0.0001% by weight, no obvious effect can be expected.

The formulation of the cosmetic composition for improving wrinkles of the present invention as described above is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the skin external ointment may be prepared to contain 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention, the flexible cosmetics fermentation of the present invention In addition to the extract, it may be prepared to contain 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.). In addition, nutrient cosmetics and nourishing cream is 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the medicinal fermentation extract of the present invention It can be prepared to contain%, essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the medicinal fermentation extract of the present invention. Massage cream is prepared by containing 30 to 70% by weight of oil such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the medicinal fermentation extract of the present invention, the pack is polyvinyl alcohol 5-20 in addition to the medicinal fermentation extract of the present invention Wash off containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in addition to the medicinal fermentation extract of the present invention in a peel-off pack or a general emulsion cosmetic containing ) Pack.

In addition, the anti-wrinkle cosmetic composition of the present invention, in addition to the components described above in each formulation, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, or fragrances, etc. It can mix and use suitably as needed.

The cosmetic composition for wrinkle improvement of the present invention has a significantly superior wrinkle improvement and durability of wrinkle improvement compared to the case of using the yeast fermentation extract or lactic acid bacterium fermentation extract of the conventional medicine extract, and is harmless to the human body.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Example 1

Commercially available Angelica, White Paper, Ginseng, Deer Antler, Red Peony, Licorice and 200 g of each pulverized powder were put in purified water or 80% methanol 3 L, and extracted by boiling in a reflux extractor with a cooling capacitor for 3 hours. Then, the filtrate was filtered through a 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then freeze-dried to dry each dry weight as shown in Table 1 below. Got. Table 1 shows the dry weight of the extract per 100 g of medicinal herbs according to the extraction solvent.

Medicine 80% methanol Purified water Donkey 8.5 g 7.7 g blank 8.4 g 7.7 g Ginseng 8.2 g 7.6 g velvet 8.4 g 7.6 g Peony 8.1 g 7.5 g licorice 8.3 g 7.9 g

Mushroom fermented product was solid fermented by adding 5% by weight of the mushroom bacteria to Angelica, white paper, ginseng, antler, red peony and licorice obtained by using 80% methanol.

Specifically, 200 g of Angelica, White Paper, Ginseng, Deer Antler, Red Peony, Licorice or Licorice were inoculated into 5% by weight of Paecilomyces japonica under constant humidity, and fermented and dried at 18-25 ° C. and 60-70% humidity for 60 days. Thereafter, 100 g of each pulverized product obtained by pulverization was put into 3 L of 80% methanol, and the mixture was boiled and extracted for 3 hours in a reflux extractor equipped with a cooling capacitor. Then, the filtrate was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and lyophilized to obtain a final mushroom bacterial fermentation product. The dry weight of the medicinal mushroom mushroom fermented dry matter is shown in Table 2 below.

Medicine Dry Weight of Mushroom Fermentation (g) Donkey 7.45 blank 7.63 Ginseng 7.83 velvet 7.85 Peony 7.62 licorice 7.52

The fermented fermented mushrooms were fermented by adding 5% by weight to YM medium, a basic medium for yeast Saccharomyces cerevisiae.

Specifically, Saccharomyces cerevisiae was inoculated in a yeast basal medium (YM medium) containing 5% by weight of mushroom fermentation at a concentration of about 10 ^{5-10 7} cells / mL, pH 5.5-6.5, temperature 25-35 C was incubated for 48 hours while maintaining the conditions of 0.05-0.5 VVM of airflow. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast cells, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal mushroom mushroom-yeast fermented dry matter. The dry weight of the medicinal mushroom bacteria-yeast fermented dry matter is shown in Table 3 below.

Medicine Dry Weight of Yeast Fermentation (g) Donkey 5.89 blank 5.71 Ginseng 5.81 velvet 5.83 Peony 5.78 licorice 5.71

Example 2

Fermented mushroom fungus of Example 1 was added to MRS medium, which is a basic medium for Lactobacillus acidophilus, by 5 wt%.

Specifically, after inoculating Lactobacillus acidophilus to the lactic acid bacteria basic medium (MRS medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The mixture was incubated for 48 hours while maintaining the temperature at a temperature of 35 to 38 ° C and aeration rate 1 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-lactic acid fermentation dried product. The dry weight of the medicinal mushroom-lactic acid bacteria fermented dry matter is shown in Table 4 below.

Medicine Dry Weight of Lactic Acid Bacteria Fermentation (g) Donkey 5.79 blank 5.71 Ginseng 5.61 velvet 5.78 Peony 5.75 licorice 5.70

Example 3

The fermented mushroom of Example 1 was added to the B medium medium Bifidobacterium longum Bifidobacterium longum in 5% by weight of fermentation.

Specifically, Bifidobacterium longum was inoculated in a lactic acid bacteria basic medium (BL medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The culture was carried out for 48 hours while maintaining the anaerobic conditions at a temperature of 35 to 38 ° C. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the bifidus bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-bifidus fermented dried product. . The dry weight of the medicinal mushroom bacteria-bifidus fermented dry matter is shown in Table 5 below.

Medicine Dry weight of bifidus fermentation (g) Donkey 5.82 blank 5.72 Ginseng 5.73 velvet 5.75 Peony 5.78 licorice 5.73

Example 4-11

The mushrooms- yeast fermentation extracts, mushrooms-lactic acid bacteria fermentation extracts of mushrooms, fungi, white paper, ginseng, antler, red liquor or licorice prepared in Examples 1 to 3 were mixed as shown in Table 6 below. (Unit is weight ratio)

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11    Donkey Fungi-Yeast 100 10 Mushrooms-Lactobacillus 100 10 Mushrooms-Bifidobacteria 100 20    blank Fungi-Yeast 20 10 Mushrooms-Lactobacillus 100 30 Mushrooms-Bifidobacteria    Ginseng Fungi-Yeast Mushrooms-Lactobacillus 20 10 Mushrooms-Bifidobacteria 100 20 10     velvet Fungi-Yeast Mushrooms-Lactobacillus 100 10 Mushrooms-Bifidobacteria 20   Peony Fungi-Yeast 100 10 10 Mushrooms-Lactobacillus Mushrooms-Bifidobacteria 10    licorice Fungi-Yeast 20 20 Mushrooms-Lactobacillus 100 10 Mushrooms-Bifidobacteria 20 10

Experimental Example  1. Collagen synthesis promoting effect

Examples 1 to 11 were added to the culture solution of human-derived fibroblasts to test collagen synthesis effects at the cellular level. The synthesis of collagen was quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).

The fermented medicinal herb extracts of Examples 1 to 11 were added to each culture medium of human fibroblast cells so as to have a final concentration of 0.05%, and then cultured for 1 day. The degree of collagen synthesis was measured at 450 nm using a spectrophotometer. At this time, the collagen synthesis degree in the same manner with respect to the sample added with the culture medium (control group), fermented yeast extract, Baeng Lactobacillus fermentation extract, vitamin C added to the final concentration of 0.005% to compare the effect Was measured.

Collagen production was measured by UV absorbance, the collagen production increase rate was calculated as the ratio of the collagen production relative to the control group and the results are summarized in Table 7 below.

sample % Increase Control group (no addition) - YM medium (final concentration 0.005%) 107.51 MRS medium (final concentration 0.005%) 108.68 BL medium (final concentration 0.005%) 109.39 Fermented yeast extract 129.14 White Lactic Acid Bacteria Fermented Extract 128.52 Example 1 153.23 Example 2 152.25 Example 3 154.17 Example 4 155.32 Example 5 153.87 Example 6 164.93 Example 7 152.54 Example 8 165.93 Example 9 154.54 Example 10 153.74 Example 11 165.43 Vitamin C (final concentration 0.0005%) 136.67

As shown in Table 7, Examples 1 to 11 of the present invention significantly increased the amount of collagen production in human-derived fibroblasts, compared to the base medium, tangyo yeast fermentation extract, Baek Lactobacillus fermentation extract, not purified at all Considering the state, it can be seen that there is a very strong collagen synthesis promoting ability in fibroblasts compared with vitamin C, a known collagen synthesis promoter.

Experimental Example 2. Collagen synthesis promoting effect according to the concentration

For the human-derived fibroblasts cultured in the same manner as in Experimental Example 1 by adjusting the concentration of Example 6 to 0.5%, 0.05%, 0.005%, 0.0005% to investigate the collagen synthesis promoting ability according to each concentration The results are shown in Table 8 below.

sample Application concentration (%) Inhibition Rate (%) Control group (no addition) - - Component of Example 6 Final concentration (0.0005%) 144.35 Final concentration (0.005%) 164.93 Final concentration (0.05%) 178.87 Final concentration (0.5%) 165.94 Vitamin c Final concentration (0.001%) 142.00

As shown in Table 8, Example 6 of the present invention was confirmed to have a very strong collagen synthesis promoting ability against cultured human-derived fibroblasts. In particular, although the ingredient of Example 6 is not a single substance but a mixture, it has the same or more collagen synthesis promoting ability at the same concentration as vitamin C, which is a single substance. Since it has a strong collagen synthesis promoting ability, it can be expected to be very useful as an antiwrinkle agent or wound healing agent.

Example 12 and Comparative Example 1

Using Example 1 to prepare a skin external ointment according to the prescription of Table 9. (Unit is weight%)

division Example 12 Comparative Example 1 Component of Example 1 10 - Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Quantity Quantity vaseline To 100 To 100

Example 13 and Comparative Example 2

Using Example 3, the cream was prepared according to the formulation shown in Table 10 below.

division Example 13 Comparative Example 2 Component of Example 3 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 14 and Comparative Example 3

Using Example 6 to prepare a flexible cosmetic water according to the formula shown in Table 11 (unit is weight%).

division Example 14 Comparative Example 3 Component of Example 6 0.2 - ethanol 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 15 and Comparative Example 4

Using Example 8, the nutritional longevity was prepared according to the formulation shown in Table 12 below. (Unit is wt%.)

division Example 15 Comparative Example 4 Ingredients of Example 8 0.1 - Waselin 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 16 and Comparative Example 5

Using Example 10, the pack was prepared according to the prescription shown in Table 13 below. (Unit is by weight.)

division Example 16 Comparative Example 5 Ingredients of Example 10 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 17 and Comparative Example 6

Using Example 11, the essence was prepared according to the formulation shown in Table 14 below.

division Example 17 Comparative Example 6 Ingredients of Example 11 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Quantity Quantity Purified water To 100 To 100

Experimental Example 3. Wrinkle improvement effect

Using the cream of Example 13 and Comparative Example 2 was carried out a panel test for the skin wrinkle improvement effect.

First, 30 healthy women from 35 to 50 years old were divided into two groups of 15 people, group 1 applied the cream of Comparative Example 2, and group 2 applied the cream of Example 13 to the facial part once a day for 3 months. It was made. After 3 months, the degree of improvement of skin wrinkles was evaluated through a subject survey. The skin wrinkle improvement and elasticity of the test subjects were determined in three stages of no improvement, slight improvement, and significant improvement compared to before use, and the results are shown in Table 15 below.

division No improvement Minor improvements A significant improvement Example 13 One 4 10 Comparative Example 2 8 6 One

As shown in Table 15, the cream of Example 13 according to the present invention was found to have excellent skin wrinkle improvement and elasticity enhancing effect.

The present invention described above is not limited to the above-described embodiments, and various substitutions, modifications, and changes can be made without departing from the technical spirit of the present invention. It is obvious to those who have it.

The cosmetic composition for improving wrinkles according to the present invention is safer on the skin and further enhances the pharmacological effect of each medicinal agent through fermentation, effectively promoting collagen synthesis, and having an excellent skin wrinkle improving effect and wound healing effect and sustainability of the effect.

Claims (11)

Wrinkles comprising fermented extract extracted by fermenting mushroom fungus fermented with at least one kind selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice as yeast, lactic acid bacteria or bifidus as an active ingredient Improvement cosmetic composition. The method of claim 1, Wrinkle improvement cosmetic composition, characterized in that the total amount of the fermented extract content is 0.0001 to 20% by weight. The method of claim 1, The fungi are wrinkles characterized in that at least one selected from the group consisting of chaga (Inonnotus-obliquus), mushroom (phellinus linteus), Cordyceps sinensis (Cordyceps sinensis, Paecilomyces japonica ), and Ganoderma lucidum Improvement cosmetic composition. The method of claim 1, The yeast is wrinkles cosmetic composition for improving wrinkles, characterized in that at least one selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids and Saccharomyces boulardii . The method of claim 1, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis cosmetics selected from the group consisting of a cosmetic composition selected from the group consisting of dogs . The method of claim 1, Bifidobacterium bacteria Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis is selected from the group consisting of cosmetic composition for improving wrinkles. The method of claim 1, The anti-wrinkle cosmetic composition is a cosmetic composition for improving wrinkles, characterized in that the external cosmetic ointment, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, or oil gel formulation. The method of claim 1, The cosmetic composition for wrinkle improvement further comprises an additive selected from the group consisting of oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, and fragrances. . a) extracting mushroom fungi by fermenting fungi selected from the group consisting of Angelica, white paper, ginseng, antler, red peony and licorice; b) fermenting the mushroom fermented product of step a) with yeast, lactic acid bacteria or bifidus bacteria; And c) separating the fermentation extract from the fermentation of step b) Wrinkle improvement cosmetic raw material manufacturing method comprising a. The method of claim 9, The method of producing a cosmetic raw material for improving wrinkles, characterized in that the fermentation mushroom mushroom step a) is a solid fermentation. The method of claim 9, Yeast, lactic acid bacteria or bifidus bacteria fermentation of step b) is a method for producing a cosmetic raw material for wrinkle improvement, characterized in that the liquid fermentation.