KR20080006691A - Herb extract for removing harmful elements accumulated in a human body and manufacturing method of the same - Google Patents

Abstract

A method for preparing a herb extract is provided to obtain the extract which is able to prevent various diseases induced by smoking by removing harmful ingredients including nicotine generated by smoking from the human body and is easily taken by being added to functional foods. A method for preparing a herb extract for removing harmful ingredients accumulated into human body comprises the steps of: (a) respectively drying Taraxacum platycarpum, Calendula arvensis, Solanum nigrum, green tea leaves, Houttuynia cordata, Lonicera japonica, Glycyrrhiza uralensis, Panax notoginseng, and Portulaca oleracea Linne to form each dried material; (b) extracting the each dried material with a solvent such as water, lower alcohol, ester, ketone, aliphatic hydrocarbon and chloroform to obtain each extract solution; (c) filtering the each extract solution to remove solid materials therefrom; (d) evaporating the solvent contained in the extract solution at a temperature of 40-60 deg.C under the pressure of 0.005-0.1 to obtain each extract; and (e) mixing the each extract in a predetermined ratio to prepare a mixture extract. A herb extract for removing harmful ingredients accumulated into human body comprises 5-40 wt.% of Taraxacum platycarpum, 0.5-10 wt.% of Calendula arvensis, 5-20 wt.% of Solanum nigrum, 5-20 wt.% of green tea leaves, 5-40 wt.% of Plantago asiatica, 5-40 wt.% of Houttuynia cordata, 5-30 wt.% of Lonicera japonica, 5-20 wt.% of Glycyrrhiza uralensis, 0.5-15 wt.% of Panax notoginseng, and 5-20 wt.% of Portulaca oleracea Linne.

Description

Herb extract for removing harmful elements accumulated in a human body and manufacturing method of the same}

The present invention relates to herbal extracts for the removal of harmful components accumulated in the human body and a method for manufacturing the same, more specifically, natural herbal medicines pogongyoung, marigold, black yam, green tea leaf, chajeoncho, three hundred seconds, honeysuckle, licorice, samchil, purslane It is manufactured by mixing at least 9 selected materials to prevent the harmful effects of cigarette smoke and to induce the harmful toxic substances in the human body caused by the harmful effects of dioxin and chemical poisons by taking various drugs such as the inhalation of pollution and the intake of plants exposed to pesticides. It relates to an herbal extract excellent in the effect of removing and a preparation method thereof.

There are many substances and elements that harm people's health, but the most damaging is tobacco. Tobacco is the largest preventable cause of death in our time. The research on the relationship between smoking and diseases has been intensively studied since the mid-1900s, and there is a continuous evidence of increasing incidences of various diseases including lung cancer caused by smoking and other health problems. In fact, about 4,000 kinds of components were found in cigarette smoke as early as present, and it is estimated that there are more than 100,000 kinds of components in mainstream smoke.

About 4000 of these components account for more than 95% of the total mainstream smoke, and thousands of other components are present in extremely small amounts. Tobacco smoke is caused by the incomplete combustion of tobacco, and if completely burned, only water and carbon dioxide are produced. Incomplete combustion is caused by incomplete combustibility of some tobacco leaf components, insufficient oxygen supply and temperature difference when the cigarette burns, and also occurs through the process of distillation, sublimation, pyrolysis, thermal synthesis, and volatility when tobacco is burned.

Tobacco smoke is an aerosol, a mixture of liquid or fine particles in a gas that passes through a Cambridge glass fiber filter (which can filter 99% or more of particles larger than 0.1 µm in diameter). A substance is called a gas or vapor component and consists of nitrogen (59%), oxygen (13.4%), carbon dioxide (13.5%), carbon monoxide (3.2%), and is toxic to bronchial cilia. Volatile components having low molecular weights such as aldehydes, ketones, alcohols and esters, etc. The material filtered by the Cambridge glass fiber filter is called Particulates phasecomponents, which accounts for about 8% of tobacco smoke, and there are at least 38 known carcinogens, including carcinogens, and carcinogens, and nicotine. And organic chemicals.

According to a recent study, cigarettes are expected to remain the biggest risk factor for human health in the 21st century, and to grow in size.

Nevertheless, it is not easy for a tobacco lover to quit smoking habits, and there is no easy means to eliminate various diseases caused by smoking while continuing to smoke.

Also, one of the many substances and factors that seriously damage people's health in our lives as much as cigarettes is dioxin.

Dioxins, once produced in nature, do not degrade well and remain stable. Accumulated in soil or sediment and introduced into organisms, it can exist for decades or even hundreds of years. Dioxins are not very soluble in water, so dioxins that enter the organism are not excreted with urine. Dioxins, however, are soluble in fat. So it accumulates well in the adipose tissue of organisms. Fish, crayfish, flying birds, mammals, and people consume dioxin by drinking water, breathing, or eating food. However, since man occupies the highest place in the food chain, the dioxin eaten by all animals is finally accumulated in the human body. Dioxin, once produced, rarely disappears and accumulates in our bodies, causing long-term health problems.

Therefore, the present invention is to solve the above-mentioned conventional problems, as a result of examining the combination of various herbal medicines, such as pogongyoung, marigold, camellia, green tea leaf, chajeoncho, buckwheat, honeysuckle, licorice, samchil, purslane, It is an object of the present invention to provide an extract that removes toxic components in the human body by finding a combination that is effective against toxic components and containing an extract having such a combination in a food.

In addition, the present invention is made by adding a solvent to each of the natural herbal medicines pogongyoung, marigold, black yam, green tea leaf, tea vinegar, weak wheat, honeysuckle, licorice, samchil, purslane and so on to make the extract, and filtered the extract to remove the solid material After each of the extracts obtained by evaporation of the solvent components are mixed in a certain ratio, it removes various toxic substances such as nicotine, tar, dioxin accumulated in the human body by inhalation or pollution of tobacco smoke, or accumulates such toxic substances in the human body. Another object of the present invention is to provide a method for preparing an herbal extract that can be prevented.

Herb extract manufacturing method for removing harmful components accumulated in the human body according to the present invention for achieving the above object is dried by drying pogongyoung, marigold, black yam, green tea leaf, chajeoncho, weak wheat, honeysuckle, licorice, samchil and purslane The first step of forming a, and the second step of forming an extraction solution in which the components of the dried product is extracted in the solvent by heating the solvent in each of the first step of drying, and the solid solution by filtering the extraction solution of the second step Mixed extract by mixing the third step of removing the material, the fourth step of obtaining each extract by evaporating the solvent component contained in the filtered extraction solution of the third step and the extract of the fourth step in a predetermined ratio It includes a fifth step of providing.

According to another aspect of the present invention, the herbal extract manufacturing method for removing harmful components accumulated in the human body is dried by drying pogongyoung, marigold, black yam, green tea leaf, chajeoncho, weak wheat, honeysuckle, licorice, samchil and purslane respectively. The first step, and the second step of mixing the dry matter of the first step in a predetermined ratio to provide a mixed dry matter, the water-soluble solvent is added to the mixed dry matter of the second step and heated to extract the components of the dry matter to the solvent A third step of forming a mixed extract solution, a fourth step of filtering the mixed extract solution of the third step to remove solids, and a solvent component contained in the mixed extract solution filtered in the fourth step And a fifth step of providing a mixed extract.

In addition, the herbal extracts for removing harmful components accumulated in the human body according to the present invention are pogongyoung 5 ~ 40% by weight, marigold 0.5 ~ 10% by weight, 5 ~ 20% by weight of yam, green tea leaves 5 ~ 20% by weight, chajeoncho 5 ~ 40 It includes 5% by weight, about 5-40% by weight, about 5-30% by weight of honeysuckle, 5-20% by weight licorice, 0.5-15% by weight, and 5-20% by weight of purslane.

Hereinafter will be described in detail a preferred embodiment of the herbal extract for removing harmful components due to smoking and a method for producing the same according to the present invention.

These examples are intended to illustrate the invention and the scope of the present invention is not limited only to these examples.

Examples of herbal extracts for removing harmful components accumulated in the human body according to the present invention are pogongyoung 5 ~ 40% by weight, marigold 0.5 ~ 10% by weight, 5 ~ 20% by weight, 5 ~ 20% by weight of green tea leaves, 5 ~ Chajeoncho 40% by weight, 5-40% by weight of 300 seconds, 5-30% by weight of honeysuckle, 5-20% by weight of licorice, 0.5-15% by weight and 5-20% by weight of purslane.

Ingredients used in the herbal extract according to the present embodiment and its working effects are as follows, and will be described in detail in the order of weak hair wheat, honeysuckle, licorice, marigold, yam, pogongyoung, green tea leaf, chajeoncho, samchil, purslane .

Houttuynia cordata Thunb (Polypara cordata Bueck) is also known as Chinese herb, succulent, triticale, effervescent, and ten, and the stem and leaves of the plant are used.

The main components are methylnonylkenone (CH ₃ (CH ₂ ) ₈ COCH ₃ ; oxidation product of decanoyl acetaldehyde) myrcene (C ₁₀ H ₁₆ ), laurin aldehyde (C ₁₁ H ₂₃ CHO), caprinaldehyde (C ₉ H ₁₉ CHO), carplic acid, and many other components, and leaves contain 2.7% of quercitrin and minerals released in water. Cuercitrin has a urinary action (100,000-fold concentration) and strong heart action, and antibacterial action against E. coli, typhoid bacteria, paratyphoid bacteria, erythropoies, gonococci, staphylococcus bacteria, filamentous fungi, and capillary strengthening. Decanoyl acetaldehyde has antimicrobial activity against non-pathogenic fungi, as well as ringworm and athlete's foot fungi, and fungi to staphylococcus aureus, gonococcus and anti-acid bacteria. Antimicrobial activity against staphylococcus is 1: 40,000 and is stronger than sulfa.

As an application, outpost is an inflammatory drug and diuretic poisoning drug. It is used for gonorrhea, urethritis, cystitis, uteritis, pneumonia, bronchitis, bite, athlete's foot treatment, antidepressant, swelling, windworm, sinusitis In the liver prescription, juice is made, but it is attached when athlete's foot, athlete's foot, hemorrhoids, snake venom and lacquer rise. The leaves and stems are urinary medicines for swelling and each other. The roots are used for wind poison and swelling.

Honeysuckle (Lonicera japonica Thunb) is another name, also known as gold and silver mistletoe, using the stem and leaves of the plant.

Flowers include luteolin (C ₁₅ H ₁₀ O ₆ ; 5,7,3,4-tetrahydroxyflavones) and inositol (C ₆ H ₁₂ O ₆ ), and the flowers are due to inositol and lead-like substances (seryl) Alcohols, sterols, arachnic acid, myristic acid, ellidinic acid, linoleic acid, linoleic acid). The leaves and stems contain 5-8% tannins, and the leaves contain loganine, about 19% nitrogen-containing substances, and roniserine, a luteolin-7-rhamnoglucoside (C ₂₇ H ₃₀ O ₁₅ 2H ₂ O). From the leaves of the same genus plant (L. morronii), the iridoid compounds roniseiroid and sweroside were isolated.

As a function, flower decoction has the effect of urinating and increasing blood sugar, and has a strong bacteriostatic effect on erythrocytes, typhoid bacteria, staphylococci, and pneumococci. Peptic gastric ulcer has a protective effect, sympathetic nerve excitement, smooth muscle paralysis action.

It is used in the treatment of motion in the early stages of wind, medicine, bleeding medicine, urine medicine, and sperm medicine. It is used for swollen, swollen, poisonous and swollen diseases. Wash. Burnt like char is used as a medicine for blood. Private prescription is used to treat cancer. It is good for stomach cancer if you eat it like water for a long time. In particular, endometritis, lymphatic tuberculosis fistula, or ophthalmitis are washed or tingled.

Licorice (Glycyrrhiza glabra L.) is another name, also known as chrysanthemum, straight licorice, or licorice, using plant roots. Roots and root stems are carved out in the autumn, trim the stems, rinse in water and dry in the sun. The rhizome and root glycyrazine content is higher in autumn than in summer. The glycyrazine content of biennial licorice is 3.8% in April, 3.7% in May, 3.3% in July, 3.8% in August, 5% in September and 6% in October. The content of glycyrrhizin along the roots is 6.91% for the root of the biennial licorice and 8.04% for the thin root. Therefore, it is reasonable to use not only the root and root stem as medicine, but also the root. Also, the skin (13.06%) is higher than the neck and water (10.42%). When peeling, only the corked skin should be peeled off, so that the skin is not damaged. Roots and stems contain 5-14% glycyrrhizin (potassium or calcium salt of glycyrrhizin), many 23%, and glabraic acid (glyciretin; C ₃₀ H ₄₆ O ₅ ).

Glycilisic acid (C ₄₂ H ₆₂ O ₁₆ ) is triterpenoids saponin, melting point 205 ℃, decomposition point 220 ℃, [d] _D ²⁰ + 58.5 ℃, well soluble in alcohol and hot water, not soluble in ether. After cooling in hot water, it hardens like grass. Decomposition of water gives tasteless glycyrrhetinic acid (C ₃₀ H ₄₆ O ₄ ) and two molecules of glucuronic acid (C ₆ H ₁₀ O ₇ ). Glycyretinic acid is a β-amirin-based triterpenoid, which has two kinds of α and β substances, which are easily changed. But its esters do not change from one another. α-glyciretinic acid is a dilute alcohol with plate crystals, melting point 283 ° C, [d] _D ²⁰ + 140 ° C (ethanol) and solved with ethanol, dioxane and chloroform. β-Glycyretinic acid is needle crystal (ethanol), melting point 296 ~ 300 ℃, [d] _D ²⁰ + 86 ℃ (alcohol), soluble in ethanol, pyridine, acetic acid and not soluble in petroleum ether.

In addition, flavono glycosides, liqueurin C ₂₁ H ₂₂ O ₉ , neoriquiritin, and lamnoricuritin (these non-sugar parts are all liqueurinine) and the corresponding galcon glycosides called isoricuitin and neo Isoricuiritin, isoram norikuiritin, and liqueur seed (the non-sugar part of these are all Isorikuuritingenin). Rhamnoricuritin is also called liqueuritoside.

More than 27 flavonoid analogs were identified by chromatograph in licorice roots.

Β-sitosterol, formmononetin, ricolycidine, lycoricone, and ricore-oligonane were identified in the roots of curved licorice. In addition, dry leaves and stems when flowering can be used as house animal feed with 14-22% protein, 2-5% oil, 36-62% nitrogen-free extract, and 5-20% ash.

Flavonoid content in licorice changes with the season of growth. The roots are the highest in the fall, and the leaves and stems are highest when the berries begin to run. The content of liqueur seeds in autumn is about 0.5%. Licuraside accounts for about 10% of the total flavonoids. There are saponaretin, glyphoside and bitexin on the stem and leaves. Saponaretin is about 50% of the total flavonoids and 3% in the outpost when fruit is opened.

Glyceramarin (bitter component of glycyridin) with a bitter taste, 2,4,4-trihydroxygalcon and its glycoside deoxystiggmasterol (melting point 135-136 ° C), β-sitosterol, essential oil (0.03%), Ascorbic acid 11-30mg%, Asparagine (1-4%), Fructose (2.4-6.5%), Glucose (2-4%) Mannitol, Starch (14-30%), Gum (1.5-4%), Yellow cattle Aspartic acid, oil, resin, tannin, protein, and amino acids include aspartic acid, proline, alanine, threonine, serine, glycine, valine, isoleucine and γ-aminobutyric acid. The most bitter substance is 8%. Among them, 2.5 ~ 3% of the water is released.

Glycirizine, which is known as an active ingredient of licorice root, has a sweet taste of about 40-50 times that of candy, and its sweetness can be felt even in a diluted solution of 100,000 times. The water solution foams when shaken, but is weaker than normal saponins and has no or hemolytic action (potassium salt is 1: 400). The glycyrrhetinic acid produced by the hydrolysis of glycyrrhizin is not sweet and hemolytic. Thus, the expectorant action of licorice is presumed to be due to glycyrrhetinic acid. Glysirizine also relieves the respiratory system.

Licorice root extract and glycyrrhizin are non-toxic and relieve poisoning by catcherchloral, strycnin, yohimbine, cocaine, morphine, codeine, atropine and luminal. Releases bacterial and viral toxins such as tetanus toxin, diphtheria toxin and snake venom. It antagonizes with acetylcholine, histamine, and allergens, and has an adrenaline-like effect.

The poisonous action of glycyrrhizin is related to glucuronic acid, a product of water degradation. Glucuronic acid is a component widely found in animal and plant systems and is combined with toxic substances in the liver to form glucuronide and excreted by urine. Therefore, glucuronic acid is used for drug addiction, alcoholism, food poisoning, jaundice, cirrhosis of the liver, chronic liver disorder, rheumatism, joint pain and neuropathy. The detoxification of glycyrrhizin is not thought to be caused solely by glucuronic acid, but is also related to the action of glycyrazine similar to that of corticosteroids. Glycirizine has the same effect as the corticosteroid desoxycorticosteroid. Glycyretinic acid reduces the excretion of sodium, chlorine and urine and increases the excretion of potassium in animal experiments.

That is, the ratio of Na / K is lowered. As such, it negatively affects potassium metabolism but positively affects sodium metabolism. It also acts to reduce the ascorbic acid content of the adrenal cortex, which sets conditions to enhance the effects of corticosteroids on water and salt metabolism. This action is also present in licorice extract and glycyrrhizin. The same thing happens when people eat.

Licorice root extract glycyrrhizin is anti-inflammatory. In other words, it has a positive effect on the treatment of experimentally induced formalin arthritis in animals. It inhibits the activity of serum transaminase and increases the activity of adenosine triphosphatase in the liver. The anti-inflammatory action of glycyrrhizin similar to desoxycorticosterone is believed to be due to the degradation product glycyrrhetinic acid. There is also a view that the corticosteroid-like action of glycyrizine inhibits the reduction of the inactivating Δ ⁴ -3 ketone moiety in vivo.

Licorice root extract has antispasmodic effect similar to papaverine on gastrointestinal smooth muscle in animal experiments. This action is due to the flavonoid component. Licorice flavonoids, which have not been used until now, was developed with known antifungal and anti-inflammatory effects. Ricuraside, a flavonoid component, has three times as strong as papaverine and has anti-inflammatory and anti-ulcer activity. And antagonists of acetylcholine and histamine.

Licorice root extract has been known to have a therapeutic effect on peptic ulcer disease in the 1950s. Thus, many experiments have been carried out to find anti-ulcer substances in licorice roots. In the 1960's, flavonoids were obtained and the components were found to work hard. In addition, recent experiments have shown that not only pneumoniae but also anti-inflammatory and anti-ulcerative action.

Licorice has a strong surface activity, so if you eat it with other copper medicines, it helps loosen the ingredients in water to help absorption. It also reduces the toxicity of the medicinal herb and changes the taste. Licorice root has a slight inhibitory effect against Staphylococcus aureus, Escherichia coli, and Lycobacterium.

When glycyrrhizin is used with anti-cancer drug thiopoxamide, anti-cancer activity reaches 85-95%.

Applications include liquorice sputum cutting, palliative action, anti-inflammatory and anti-inflammatory action, and antihistamine action.

Therefore, it is used as a phlegm acupuncture in the upper gastrointestinal disease. It can also be used as a poison or medicine. Nowadays, it is widely used for gastroduodenal ulcers, Edison disease, bronchial asthma, jaundice and hepatitis, eczema and other skin diseases. Glycyrrhizin is used as a supplement to increase the activity of anticancer drugs.

In motion therapy, the roots are often used for the purpose of mitigating and harmonizing the actions of other medicines in the prescription. In other words, when used with a hot medicine to relieve heat, and when used with a cold to relieve the cold.

Like palliative medicine or pain 멎 jinjak, sputum pill, cough 멎 yipo, poison pool medicine stomach cramps, stomach pain, sore throat, stomach ulcers, duodenal ulcers and enter into a poisonous prescription.

Calendula officinalis L., also called calendula, is another name that uses petals that are dried in the shade.

The main ingredients are carotene, lycopene (melting point 175 ℃), violaxanthin (C ₄₀ H ₅₆ O ₄ ; melting point 207 ~ 208 ℃), rubyxanthin (melting point 160 ℃), neoricopin A, citraxanthin ( Flavonoids such as melting point 163 ~ 164 ℃) and clavochrome (melting point 184 ~ 185 ℃). The content of carotenoids is about 3%. Flowers contain 0.02% essential oils, 3.4% resins (1.5% of which are nitrogenous mucus), 0.64% albumin, 6.84% organic acids calculated from malic acid, small amounts of salicylic acid and alkaloids. Above ground, calenden (C ₂₃ H ₃₈ O ₇ ), saponin (when decomposes into oleanolic acid and glucuronic acid), arnidiol, paradiol, tannin (6-7%), unsaturated triterpenediol There is this. Seeds include alkaloids, oils (primarily glycerides of lauric acid and palmitic acid), and seryl alcohol and phytosterol in the unsaponified portion of oil.

As a function, flower decoction, alcohol extract, and juice have a calming effect on the central nervous system in animal experiments and lower reflex excitability. It also lowers blood pressure, hardens heart contractions, and slows down the heart. There is also bactericidal action.

The application is to make plaster and apply it to burns, ulcers, dentitions, sharp peaks, and sharp peaks. Also, rainforest medicine and tinctures are affected by ophthalmitis, alveolar pylori and uterine fistula, cervical erosion, and trichomoniasis vaginitis (2% tincture). Rainforest medicine and tincture are used for gastric duodenal ulcers, hepatitis, streptococcitis, and saccharitis as inflammatory drugs and fever-fighting drugs. It is also used for high blood pressure and heart disease. The combination of pollen and nicotinic acid is used as a symptomatic medicine for inoperable stomach cancer. If you use this medicine, symptoms of intoxication, indigestion, belching, nausea will be less, and the taste will be better.

Solanum nigrum L. is also known as lats, yonggyu and kamjong. The fruit is round and ripened in black. When the outpost (Yonggyu) is in bloom, the stem is dried in the shade and used.

The main ingredients in the outpost are steroid alkaloids, small amounts of atropine and nicotine. Steroidal alkaloids include solanine (C ₄₅ H ₇₃ O ₁₅ N; solanidine-β-l-rhamnoside-d-galactosid-d-glucoside), solasodine, solanigrin and the like. Outposts also include flavonoids, rutin, asparagine, solangustin, cytosterol 7-10% tannins, saponins (diosgenin, tigogenin), and organic acids. Coconut has similar ingredients to the outpost. Ripe fruits include glycoalkaloids, saponin and anthocyanin pigments, β-carotene (318-503 mg% as carotene), and 1,630 mg% ascorbic acid. Other sources include solasonin (0.2%) and solamargin (0.25%). The leaves contain between 24 and 184 mg of ascorbic acid and 50 mg of carotene. The roots also contain alkaloids and saponins. As a function of the outpost decoction has typhoid bacteria, staphylococcus aureus, Pseudomonas aeruginosa, erythropoiesis, E. coli, fungi. It also has anti-inflammatory and hypoglycemic effects. The alkaloid component promotes histamine degradation in lung and bronchial tissues, and therefore coughs have phlegm. It also lowers blood pressure. Very small amounts of atropine make the pupils larger, which may result in better vision, even temporarily, in patients with severe Batu Boi who suffer from mild paralysis of eye control.

The application is used to restore fatigue from motion therapy to tonic, fever and urine. Leaves and stems are attached to various wounds and cuts, and fruit brightens the eyes. In civilian use, it is used as a tonic and urinary medicine for sinseokjung and bite. Starch juice is used for colds as a sedative, cough and sweating medicine. Flowers are sputum, leaves are astringents, blood drops and diarrhea. Leaf and Fruit Weinum is an antiseptic and inflammatory drug used for hemorrhoids, ulcers, wounds, swelling, ringworm, eczema, navel, soles, and strokes. It is also used for head pain, rheumatism, gout, and tumors.

Dandelion (Taraxacum platycarpum H. dahist) is also known as pogongyoung, and it is used in the sun when the flowers are rooted and dried in the sun.

The main component is lactosphyrin (which is decomposed into lactocin and p-oxyphenylacetic acid), taraxasin, γ-amirin, taraxerol C30H50O, caffeic acid, β-cystoterol, stigmasterol, p- Kumaric acid, serotonic acid, tannins, and choline. Arnidiol C30H50O2 (melting point 257 ° C), paridiol C30H50O2 (melting point 236 ° C), triterpene alcohol, and delacsarsterol C30H50O (melting point 220 ~ 221 ° C), pseudotaraxa There is sterol C30H50O (melting point 217 ~ 219 ℃), the yellow of the flower is mainly C40H56O3. The outpost contains the flavonoids cosmosine, luteolin-7-glucoside, 6-10 mg% carotene, 6-62 mg% ascorbic acid and vitamin B1 B2 D on the leaves.

As a function of the outpost has an Idam activity. In addition, the secretion of gastric juice is faster and urinary action has a therapeutic effect on the portal bite.

Its application is hepatobiliary disease medicine, physiological digestive medicine, rice flavoring medicine, and is widely used in the nutritional diet of patients with recovery period.

In motion therapy, it is a placebo, urinary medicine, or blood pill, indigestion, gastritis, stomach ache, lactation, stiffness and squeezing of the urine, which is used to stop colds, sore throat, and eye disease.

As a folk medicine, it is used for cough, pulmonary tuberculosis and urinary medicine, nephropathy, and inflammatory medicine for colitis and gastric ulcer.

Thea sinensis L is a evergreen evergreen tree. The leaves are thick and long oval. White flowers bloom in autumn Fruits are flat round shape with three corners. Jeolla-do, there are some that grow in Gyeongsang-do, but they are often planted.

Tea leaves: The processing method is divided into green tea, black tea, flower tea (hwacha).

Green tea is picked up and steamed in medium or hot air to inactivate oxidase and then dried. When the shoots sprout in spring, they block the sunlight to prevent tannins, and after picking the cultivated shoots, they are steamed in the middle and dried. Black tea is picked and dried in half, then crushed and stacked overnight. At this time, the tannin is oxidized by the action of the enzyme to have a red color. This is dried by hot wind. Flower tea is a mixture of leaves and flowers, piled up, absorbed with floral fragrance, and dried.

The main components of the leaves are 1-6% of purine bases such as caffeine (C ₈ H ₁₀ O ₂ N ₄ ), xanthine (C ₅ H ₄ O ₂ N ₄ ) and theophylline. It also has 9-15% tea tree tannins. Among them, soluble tannin is about 3% and insoluble tannin is 9.9%. Tea tree tannin (C ₂₂ H ₁₈ O ₁₀ ) is a molar ester of teacatechol, ie, 3-galloyl-l-epi-gallocatechol. In addition, the steroid theasterol (C ₂₉ H ₅₀ O), a-spinasterol and triterpenoids β-amirin (C ₃₀ H ₄₈ O) Palthreubin (C ₃₀ H ₅₀ O) and carotene vitamin C, Quercetin, camphorol and the amino acids arginine and theanine. The sweetness of tea is mainly due to theanine. Green tea contains about 0.006% of essential oils, the main components of which are α-β-hexenol (CH _{3 -} CH _{2 -} CH = CH _- CH _{2 -} CH ₂ OH) (50-60%), α-β-hexenol (CH _{3 - (CH 2) 2 -CH} = CH - CH 2 O) (5%), iso-butyl aldehyde, isobutyl aldehyde valeryl, methyl ethyl acetic second aldehyde, valeric acid, salicylic acid, benzyl alcohol, linalool And so on. In addition to caffeine, alkaloids include theophylline and theobromine, and include xanthine, adenine, hypoxanthine, paraxanthine, methylxanthine and isatin. It also contains lecithin, nucleotide adenine, nucleotide cytosine, nucleorotate and coumarin (umbeliferone-7-glucoside) containing iron and manganese, vitamins B ₁ , B ₂ , B ₃ , B ₅ , K ( 156 ~ 233mg%). And essential oils, flavonol glycosides (quercetin and camphorol derivatives). Seeds contain 22% caffeine oil (18% in the seed) theasaponin (C ₅₇ H ₉₀ O ₂₆ ). Theasaponin is hydrolyzed to theaasapogenol (C ₃₀ H ₅₀ O ₆ ), glucuronic acid (C ₆ H ₁₀ O ₇ ), galactose, arabinose, xylose, and angelic acid. Seeds contain 33% starch and 8.5% protein. Stems, roots, and seeds contain steroid saponins, especially in seeds (9-10%).

The application has been used as a medicine for a long time. It has been used to excite the nervous system and strengthen mental and physical activity. Feeding children with low levels of prothrombin in their blood increases their content. Tea Leaves: Used as a monthly charge for caffeine and tea tree cadkin. These drugs are used for hemorrhagic predisposition, retinal bleeding, photopathy, and high blood pressure when the weakness of the blood vessels and the permeability are destroyed. Caffeine is used for tension-induced weakness in general, low blood pressure, acute infectious diseases, and mental and physical fatigue when heart activity is weak. It is also used as a poison medicine for alcoholism and opioid poisoning. Theophylline improves the blood circulation of the heart and is used as a urine medicine for blood circulation disorders that result from dysfunction of the heart and kidneys. Theobromine is used in the preparation of drugs called diuretin and theophylline, which are used for cardiovascular disorders, hypertensive cardiac edema, and opiphyline, which are used for bronchial asthma and angina.

Green tea leaves are immediately dried without picking young leaves. The fragrance is weak but physiologically more active. Since green tea has antimicrobial activity, it is used as a decoction for proper treatment. In other words, add 2L of water to 100g dry green tea leaves, soak for 20-30 minutes and boil for an hour. Filter it with a double layer of cloth and take 1 to 2 spoons a day 20-30 minutes before eating. 5-10 days for Venus Ely, 16-20 days for Chronic. It is also used during colitis, proctitis, and indigestion. Dark tea is also used to prevent sunburn in advance. Before leaving the sun, wash and rub exposed areas with dark tea. Relieves pain and heat even when burned by sun. There are many ways to drink tea. You can drink dark tea with milk and drink it with hot water or candy. Drinking tea in the morning is effective because it contains a lot of ingredients necessary for human activities because it positively affects daily life.

Plantago asiatica L. (P.major L. var.asiatica) has different names, such as long janggu and baejanggi, and cuts the bottom of the leaves when they bloom.

The main components of the leaves are auribin glycoside aucvin (C ₁₅ H ₂₄ O ₉ ), catalpol and flavonoids flantaginine (scutellalein-7-glucoside), homoplanetaginine (hispitolin-7 glucoside There is). Planttagin (5,6,4-trimethylscooterlane-7-glucoside). There are also apigenin, luteolin, nefetin, and 6-oxyluteolin glycosides. The leaves also contain palmitic acid esters of ursolic acid, gentriacontane (C ₂₃ H ₄₈ ), β-sitosterol, stigmasterol and β-sitosterol. There are also enzymes (emulsin and inverotene), carotene and ascorbic acid. Seeds contain acubin and mucus. Partial hydrolysis of the mucus resulted in the disaccharide I [O-β-D-xyclopyranosyl- (1 → 2) -D-xyclopyranose], II [O-β-D-xylopyranosyl- (1). → 4) -D-xyclopyranose] and III [(O-β-D-glucopyranosyl-uronic acid- (1 → 5) -L-arabinopyranose]]. Nolanic acid, succinic acid, choline, adenine, vitamins A and B _1. The outpost contains polysaccharides that are dissolved in water, and polysaccharides contain up to 28% ash and only about half are removed by electrodialysis. Time-refined ash yields 0.4-0.5% Polysaccharides consist of 80-82% pectic acid and 5-6% galactoaraban 4-5% galactan.

When hydrolyzed, pectinic acid produces 78-83% of galacturonic acid, galactose, arabinose, rhamnose, small amounts of glucose, xylose and three other sugars. That is, the polysaccharide is composed of all nine sugars and minerals. Mineral-free polysaccharides contain 66-68% anhydrous galacturonic acid. Hydrolysis of this leads to galacturonic acid. According to other data, the relative content of polysaccharides of rhamnose, arabinose, galactose, xylose, glucose and mannose is 6: 6: 3.5: 2.5: 1: 1. That is pectin mainly made of galacturonic acid. In comparison with P.major, which grows in Europe from the standpoint of chemical classification, the plantain has one more iridoid glycoside and 6-oxyluteolin glycoside. The content of polysaccharides varies between 8 and 19% depending on the growing season. It is the highest when the leaves start to grow gradually in spring and the flower beds begin to emerge. When the fruit is ripe and the flower stalk dries, its content is lowest. Considering the productivity of raw materials, it is recommended to collect them in June, from the time the buds sprout to the flowers.

In effect, outposts and planttagins act on the center of the hob in animal experiments, slowing and deepening the hob. It also increases the secretion of mucous membranes, increasing the secretion of bronchial mucosa and digestive juices. Seed mucus has a relaxing and heat-lowering effect. The antitussive expectoration of plantain is different from saponin with hemolytic action and irritation to mucous membranes. It is good for children's cough medicine because it has no hemolysis and no side effects. Plantain also has a peeing action. In addition to increasing water excretion, it also promotes excretion of urea, sodium chloride and uric acid. Polysaccharides are anti-inflammatory and epithelial. Outpost extracts have anti-inflammatory and epithelial activity in small wounds in animal experiments. Polysaccharides isolated from outposts are less toxic. Polysaccharides in rats 1 to 3g / kg, dogs to 2 ~ 5g / kg for 14 days, there is no poison. Polysaccharides inhibit gastric ulcer formation in rats by butadione. Feeding dogs also increases the secretion of gastric juice by 2.5 times and increases the free acidity and total acidity of gastric juice by 20-30%. However, there is no effect on the protein lytic activity of gastric juice. Polysaccharides slow the movement of matter three to four times through the stomach. Mycelial action of polysaccharides on isolated intestines of rats is also shown in 1: 10,000 solutions.

In addition, polysaccharides have antifungal, anti-inflammatory and epithelialization effects, making them a good treatment for ulcers. Splitting minerals in polysaccharides does not have this effect. Therefore, it is considered that the polysaccharide combined with the inorganic material is a biologically active component. Polysaccharides have been shown to be therapeutic in clinical trials. If a stomach duodenal ulcer patient eats 0.5-1 g at a time three times a day, pain disappears after 5-7 days, and ulcer symptoms disappear after 15-20 days, and the acidity of gastric juice is normal. The action of polysaccharides is to lower the elevated acidity and increase the lower acidity to normalize the secretion of gastric juice. In particular, patients with normal or low acidity of gastric juice have good therapeutic effect.

Applications include polysaccharides or juices derived from outposts used for duodenal ulcers and chronic gastritis with or without acidity in gastric juice. Outpost decoction is also effective for nephropathy.

In copper therapy, outposts are used for gastrointestinal and other arteriosclerosis and diabetes. Mr. Moon drinks from eye diseases and nervous system diseases.

Gynura segetum Merr. (G.pinnatifida DC.) Digging roots in autumn, rinsing with water, cutting and drying in the sun.

Its main ingredient is alkaloid cesionine C18H25O5N3, tannins in the root. Pinnathiphytin, formerly known as Tossamchil's pique, is senesionine. Senesionine hydrolyzes with alcoholic potassium hydroxide to form retronesin C8H13O2N and transcenesionine acid C10H16O5 (integerminic acid). Retronecine and senesionine lactone C10H14O4 (platinine acid).

Root decoction and fluid extract have a clear bloody effect in animal experiments. Senesionine reduces blood bleeding time and has a blood clotting effect. Therefore, the physiologically active component is considered to be senesionine.

It is used for blood bleeding, nosebleeds, uterine bleeding and other internal bleeding.

In folk medicine, the leaves are used as poison poison for insect poisoning, snake venom, and bee venom.

Purslane (Portulaca oleracea L.) is a perennial grass, 15-30 cm high. Oval fat leaves stick together. Pour in the summer, slightly simmer in hot water and dry in the sun. Machihyeon, the leaves are green, the stems are red, the flowers are yellow, the roots are white, and the seeds are black.

The main components are dopamine C ₈ H ₁₁ O ₂ N, and a small amount of dopamine C ₉ H ₁₁ O ₄ N in the outpost, almost equal to l-noradrenaline C ₈ H ₁₁ O ₃ N 0.25%. The activity of noradrenaline is the same as for synthetic products. There are also potassium salts (nitrates, hydrochlorides, sulfates), calculated as k ₂ O, 1% for raw, 10-17% for dry), large amounts of organic acids (apple, lemon, and cinnamic acid), amino acids (glutamic acid, aspartic acid). , Alanine), simplicity glycosides, anthraquinone glycosides, and alkaloid reactions.

Fresh outposts contain 94-96% moisture and 4.5-6.6% dry matter. Dry matter is crude protein 14 ~ 24%, crude fat 3.7 ~ 5.8%, crude ash 25 ~ 27%, monosaccharide 2 ~ 3%, candy 4.4 ~ 6%, hydrolyzable polysaccharide 9 ~ 14%, starch 0.2 ~ 2.5%, Vitamin B ₁ and C, carotene 9.4 ~ 17mg%, nicotinic acid 25 ~ 50mg%, tocopherol 2 ~ 6mg%, trace elements zinc, copper, manganese, nickel, iron, and the like. In addition, saponin, tannins are contained.

Outpost alcohol extract has a bactericidal action against Escherichia coli, erythrocytes, typhoid bacteria. Clinical studies have shown a clear therapeutic effect on trichomoniasis and bacterial dysentery, but not on avemic dysentery.

Shrink the uterus of the rat and the rabbit. Diuretic action caused by potassium salt.

In the treatment of copper, detoxification, drainage, and diuretic effect, dysentery, enteritis, bacterial strains are eaten, swelling, alone, scabies, eczema, etc. are eaten or washed. It is also used to solve bugs and snake venom and is effective in various diseases and jijang cancer.

A first embodiment of the method for preparing herbal extract for removing harmful components accumulated in the human body according to the present invention will be described in more detail below.

The first step is to dry the pogongyoung, marigold, black yam, green tea leaf, weak wheat, honeysuckle, licorice, chajeoncho, samchil, purslane respectively to form a dry matter, drying of the materials listed above is carried out in the shade without sunlight. . When dried in the sun, the active ingredients are destroyed or evaporated, thereby reducing the efficacy or reducing the amount of extract. Drying is preferably performed until the moisture on the surface of the material is almost evaporated. For example, in the case of blades of grass, drying is performed until the tip is slightly curled. Since the amount of the solvent added to the dried material varies depending on the weight of the dried material and the drying amount is appropriate, the amount of the solvent can be reduced, so it is preferable to appropriately perform the drying step of each dried product.

In the second step, a solvent is added to each of the dried products of the first step, and the components of the dried product are extracted into the solvent to form an extraction solution. The amount of solvent added to each dried material is mixed about 1.5 to 2.5 L of solvent per 100 g of dry matter.

The solvent of the second step is at least one selected from the group consisting of water, lower alcohols, esters, ketones, aliphatic hydrocarbons and chloroform, preferably water, ethanol, methanol, isopropyl alcohol, n-butanol It consists of at least one selected from lower alcohols, esters containing ethyl acetate, ketones containing cyclo-butanol, cyclo-pentane, n-hexane, chloroform and acetone. The greater the polarity of the solvent of the second step, the more extraction solution can be obtained. In addition, there is a difference in the amount of the extraction solution according to the production site or the collection time of each dry matter of the first step.

In the method of heating each dried material selected from the above-listed solvents, heating is performed for about 1-5 hours under atmospheric pressure using a mantle provided with a reflux condenser. By lowering the atmospheric pressure below atmospheric pressure, the heating time can be shortened. Therefore, if necessary, the air pressure can be lowered to 0.1 atm or less, which greatly shortens the heating time. What is necessary is just to adjust heating temperature suitably in consideration of the boiling point of the solvent used. In general, heating at atmospheric pressure for 1-5 hours dissolves the extract components from the dry matter and may reduce or extend the heating time as necessary.

The third step is to remove the solid material by filtering the extraction solution of the second step. The extraction solution through the second step is present in a liquid state in which the dry matter and the solvent are mixed, and the dried product in the residue state in the extract solution is filtered by a filter to obtain a filtered extract solution in which the dry ingredients are dissolved.

The fourth step is to obtain each extract by evaporating the solvent component contained in the filtered extraction solution of the third step is a step of heating to a temperature of 40 to 60 ℃ at 0.005 to 0.1 atm. If the evaporation temperature is increased to 60 ° C or higher, there may be a chemical change in the extraction solution or the components of the extraction solution may come out when depressurizing, and when the temperature is 40 ° C or lower, excessive time is required for evaporation. do.

The fifth step is to provide a mixed extract by mixing the extract separated from the extraction solution of the fourth step in a predetermined ratio.

In other words, each extract obtained from the fourth step is poongyoung 5-40% by weight, marigold 0.5-10% by weight, 5-20% by weight in black, 5-20% by weight of green tea leaves, 5-40% by weight of chajeoncho, 5 ~ weak wheat 40% by weight, 5-30% by weight of honeysuckle, licorice 5-20% by weight, 0.5-15% by weight of samchil, 5-20% by weight of purslane is preferably mixed.

Next, the mixed extract produced in the fifth step is prepared as a functional food by containing 5 to 70% by weight of the mixed extract in a conventional food as an additive object.

When the amount of the mixed extract contained in the food is less than 5%, the removal effect of the harmful component is insufficient, and when the amount of the mixed extract is added 5% or more, the removal effect of the harmful component is remarkable. It is preferable to make it contain%.

In addition, as the functional food to which the mixed extract can be added, there are confectionery such as yogurt, candy, cookies, jelly, chewing gum, soft drinks, etc., and can also be applied in the form of tobacco additives. Moreover, it can also add as a raw material mix | blended with medicine, herbal medicine, etc.

In addition, the mixed extract prepared in the fifth step may be formulated in the form of powder, granules, tablets, capsules and the like by mixing with excipients lactose, corn starch, cellulose of a microcrystalline structure, magnesium stearate and the like.

The second embodiment of the method for producing a herbal extract for removing harmful components accumulated in the human body of the present invention is dried pogongyoung, marigold, black yam, green tea leaf, buckwheat, honeysuckle, licorice, chajeoncho, samchil, purslane to form a dry matter The first step, the second step of mixing the dry matter of the first step in a predetermined ratio to provide a mixed dry matter, and the water-soluble solvent is added to the mixed dry matter of the second step and heated to extract the components of the dry matter to the solvent A third step of forming a mixed extract solution, a fourth step of filtering the mixed extract solution of the third step to remove solids, and a solvent component contained in the mixed extract solution filtered in the fourth step The fifth step is to provide a mixed extract.

This embodiment is the same as in the first embodiment except that the dried solution of each material is pre-mixed in a predetermined ratio to form an extraction solution, followed by filtration and evaporation, and thus a detailed description thereof will be omitted. .

The mixing ratio of the dry matter of the second step of the present embodiment is 5-40% by weight poongyoung, 0.5-10% by marigold, 5-20% by weight in black, 5-20% by weight of green tea leaves, 5-40% by weight of chajeoncho, weak hair 5 It is preferable to form each mixed dry matter in the range of the ratio of -40 weight%, 5-30 weight% of honeysuckle, 5-20 weight% of licorice, 0.5-15 weight% of samchil, and 5-20 weight% of purslane.

Experiments were performed to compare the yields of herbal extracts according to various solvents used in the preparation of herbal extracts, that is, herbal extracts according to the present invention.

① Experiment Method

The experiment was performed by adding 2 liters of solvent per 100 g of the dried substance and extract, and heating the dried substance and extract and solvent at atmospheric pressure in a mantle provided with a reflux condenser for 2 hours, and removing the residue on top of the heated mixture. The liquid residue in the lower part was filtered and each extract obtained from the dried product and a mixed extract obtained from the extract were obtained as a product.

The respective extracts and mixed extracts are evaporated under a condition of 0.008 atm (5 mmHg) and 50 ° C. to finally obtain dry extracts and mixed extracts of each dry extract.

② Experiment Result

The amount of the dry extract and the dry extract in the mixed form obtained by each type of solvent was high in the case of the preparation by adding water, ethanol and methanol as a solvent. It is effective to use water and ethanol as solvents.

Table 1 shows the yield values obtained for each type of solvent.

TABLE 1

Type of solvent Yield (%) Type of solvent Yield (%) water 10 Acetone 3 ethanol 8 chloroform 4 Methanol 8 cyclo-butanol 4 Isopropyl Alcohol 6 cyclo-pentane 2 n-butanol 6 n-nucleic acid 2 Ethyl acetate 7

Experiments performed to explain the effect of the herbal extract prepared by applying water and ethanol confirmed from the results of Table 1 and the results are described in detail below.

end. Test of nicotine concentration in blood

1. Experimental conditions

① Test Animal

ICR Mouse of Wister strain male rats (6 weeks old)

② Test environment

5 animals each in the wire mesh breeding room of the animal room set at room temperature 23 ± 2 ° C, humidity 55 ± 10%, ventilation number 13 times / hour, lighting time 12L / 12D hours / day (8 am on, 8 pm off) Accepted. The feed was made free of solid feed with water.

③ Method of administration

Adapted to the test environment for 2 weeks, animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / mari solid feed and orally administered to the body as free food with water.

④ Dosing time

Administration was performed at 2 pm and the period was 7 days. At the end of dosing and at the end of the test, 1 ml of blood was drawn from the heart as a sample for nicotine measurement.

⑤ Observation method

Dosing days were observed immediately after administration and in the evening and once a day thereafter. Observation was carefully conducted from outside the breeder.

2. Experimental Results

① nicotine concentration in blood

Blood was adjusted to pH 9 with dilute ammonia water. Next, the resultant was maintained on an outer column (EXTRELUT COLUMN; manufactured by Merck Co., Ltd.) for 15 minutes, eluted with 15 ml of ethyl acetate, the eluted acetate was distilled off under reduced pressure, and 100 µl of ethyl acetate was added to the resulting residue. The sample was analyzed.

The analysis was performed using gas chromatography (US 5890 SERIESП, FID) with a column filled with 2% Thamon 1000 + 1% KOH chromosorb WAM (8 / 100Mesh). A glass column 2 m long and 3 mm in diameter was used.

The column temperature was 100 ° C., the carrier gas used nitrogen (N ₂ ), and the flow rate was 60 ml / min. Table 2 shows the result of administering the herbal extract obtained from the example for 7 days, and the nicotine concentration was 30 mg / kg / 10ml and was intraperitoneally administered.

TABLE 2

Paralysis symptoms Activity start time Activity end time Death time Nicotine concentration in blood (㎍ / ㎖) G1 One 27 ' 2'10 ' 5'50 ' recovery 0.024 2 36 ' 2'44 ' 6'10 ' recovery 0.027 3 43 ' 3'25 ' 6'51 ' recovery 0.015 4 43 ' 3'50 ' 6'56 ' recovery 0.021 5 33 ' 3'52 ' 6'35 ' recovery 0.017 G2 One 22 ' 4'42 ' 6'45 ' 31'18 ' 1.037 2 26 ' 4'46 ' 6'35 ' recovery 0.014 3 20 ' 5'40 ' 7'25 ' recovery 0.012 4 24 ' 5'47 ' 7'20 ' recovery 0.013 5 23 ' 4'46 ' 6'47 ' recovery 0.019 G3 One 23 ' 35 ' 56 ' 1'33 ' 12.178 2 33 ' 45 ' 1'15 ' 1'35 ' 12.183 3 29 ' 31 ' 1'20 ' 1'50 ' 12.987 4 25 ' 54 ' 1'35 ' 1'57 ' 12.969 5 22 ' 51 ' 1'50 ' 2'10 ' 11.719 G4 One 33 ' 2'50 ' 4'20 ' 6'15 ' 4.019 2 25 ' 2'40 ' 4'50 ' 7'18 ' 3.679 3 24 ' 2'45 ' 4'47 ' 6'45 ' 4.095 4 21 ' 2'37 ' 5'10 ' 6'25 ' 4.074 5 33 ' 2'35 ' 4'15 ' 6'54 ' 4.117

Reference-G1: Nicotine administration after pretreatment of the herbal extract obtained from the Example.

G2: After pretreatment of the herbal extracts obtained from the examples, administration of the herbal extracts obtained from the examples after nicotine administration.

G3: control nicotine administration.

G4: Administration of the herbal extract obtained from the Example after administration of the control nicotine.

The test population was tested five dogs in each group

② autopsy

No visual abnormalities were found in each individual when recovered from the autopsy at the end of dosing.

I. Measurement experiment of urine nicotine concentration

The urine nicotine concentration was measured in the same manner as the test animal, test environment, administration method, administration time, observation method, and analysis method of Experiment 1, and the results of the measurement test of nicotine concentration in urine are described below.

Test result of nicotine concentration in urine was adjusted to pH 9 by adding dilute ammonia water to urine, maintained for 1 minute in an EXTERELUT COLUMN (manufactured by Merck), eluted with 15 ml of ethyl acetate, and then eluting acetate was Distilled off. 100 µl of ethyl acetate was added to the resulting residue to dissolve and used as an analytical sample. Table 3 shows the results after administration of the herbal extract prepared according to this example for 7 days, and was administered nicotine concentration 30mg / kg / 10ml intraperitoneally.

TABLE 3

Paralysis symptoms Activity start time Activity end time Death time Nicotine Concentration in Urine (µg / mL) G1 One 22 ' 2'20 ' 5'30 ' recovery 5.524 2 34 ' 2'34 ' 6'30 ' recovery 5.527 3 43 ' 3'45 ' 6'31 ' recovery 5.515 4 46 ' 3'40 ' 5'36 ' recovery 5.531 5 30 ' 3'32 ' 6'15 ' recovery 5.517 G2 One 23 ' 4'32 ' 6'35 ' recovery 5.537 2 23 ' 4'36 ' 6'55 ' recovery 5.514 3 34 ' 5'50 7'55 ' recovery 5.512 4 21 ' 5'17 ' 7'55 ' recovery 5.513 5 20 ' 4'26 ' 6'37 ' recovery 5.519 G3 One 20 ' 30 ' 57 ' 1'43 ' 0.021 2 33 ' 55 ' 1'35 ' 1'55 0.031 3 23 ' 41 ' 1'50 ' 2'30 ' 0.037 4 24 ' 54 ' 1'35 ' 1'57 ' 0.021 5 25 ' 55 ' 1'55 ' 2'30 ' 0.029 G4 One 31 ' 2'30 ' 4'10 ' 6'45 ' 0.219 2 23 ' 2'30 4'45 ' 7'25 ' 0.279 3 23 ' 2'55 ' 4'37 ' 6'15 ' 0.215 4 23 ' 2'47 ' 5'50 ' 6'55 ' 0.214 5 30 ' 2'55 ' 4'45 ' 6'14 ' 0.217

All. Carbon monoxide concentration test in blood

1. Experimental conditions

① Test Animal

ISR mouse of Wister strain male rat (6 weeks old)

② Test environment

5 animals each in the wire mesh breeding room of the animal room set at room temperature 23 ± 2 ° C, humidity 55 ± 10%, ventilation number 13 times / hour, lighting time 12L / 12D hours / day (8 am on, 8 pm off) Received. Feed was freely fed solid feed with water.

③ Method of administration

Adapted to the test environment for 2 weeks, animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / mari solid feed and orally administered to the body freely with water.

④ How to expose carbon monoxide (C0)

On the day after the end of dosing, each animal is placed in a chamber for exposure (Sugiyamage), and O ₂ A mixed gas of 21% and 100 ppm of CO was introduced into the pump and sucked for 5 minutes. The concentration in the chamber was examined with a carbon monoxide meter (COM-4, manufactured by Komei Rikagaku Kogyo, Japan).

⑤ Measurement of carbon monoxide-hemoglobin (CO-Hb) concentration in blood

Immediately after the end of the exposure, blood was drawn from the heart, 0.5 ml of blood was placed in a 5 ml reaction vial, and a drop of octyl alcohol and 0.25 ml saturated aqueous potassium ferricyanate solution were added. The reaction solution was capped and shaken for 5 minutes. It was used as a sample for gas phase analysis.

For analysis, use gas chromatography (HP 5890 SERIESП, TCD, USA) and the column is molecular sieve.

A glass column 2 m in length and 3 mm in diameter filled with 5 A (3/60 Mesh) was used. The column temperature was 60 ° C., the carrier gas used hydrogen (H ₂ ), and the flow rate was 50 ml / min.

2. Experimental Results

① the concentration of carbon monoxide in the blood

The results of measuring the concentration of carbon monoxide (CO) in the blood after the test, see Table 4, Table 4 is the result of administration of the herbal extract prepared according to the present embodiment for 7 days.

 TABLE 4

  CO-Hb concentration in blood (%)   G1 One 49.2 2 48.9 3 47.1 4 48.8 5 49.0   G2 One 49.3 2 48.8 3 49.6 4 48.9 5 49.2   G3 One 69.5 2 70.5 3 69.3 4 69.0 5 68.9   G4 One 59.5 2 59.0 3 59.6 4 59.1 5 59.5

② autopsy

At the end of the autopsy, no gross abnormalities were found in each individual in each group.

la. Test for Tar

1. Experimental conditions

① Test Animal

ISR mouse of Wister strain male rat (6 weeks old)

② Test environment

5 animals each in the wire mesh breeding room of the animal room set at room temperature 23 ± 2 ° C, humidity 55 ± 10%, ventilation number 13 times / hour, lighting time 12L / 12D hours / day (8 am on, 8 pm off) Received. Feed was freely fed solid feed with water.

③ Method of administration

Adapted to the test environment for 2 weeks and animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / marion solid feed and orally administered to the body as free food with water. Tar was administered into the stomach using a Stomach tube.

④ Observation Method

Dosing days were observed immediately after administration and in the evening and morning and evening from the following day. Observation was carefully conducted from outside the breeder.

2. Experimental Results

The results of the measurement of tar after the experiment are shown in Table 5, and Table 5 shows the results after administration of the herbal extract prepared according to the present example. The concentration of Tar was administered intraperitoneally at 30 mg / kg / 10 ml.

TABLE 5

time 1 day 2 days 3 days 4 days 5 days 6 days 7 days   G1 One survival survival survival survival survival survival survival 2 survival survival survival survival survival survival survival 3 survival survival survival survival survival survival Dead 4 survival survival survival survival survival survival survival 5 survival survival survival survival survival survival survival   G2 One survival survival survival survival survival Dead 2 survival survival Dead Dead Dead 3 survival survival survival survival survival survival survival 4 survival survival survival Dead Dead 5 survival survival survival survival survival Dead

References: G1: The herbal extract obtained from the example is mixed with a general feed, followed by pretreatment of tar.

G2: Tar administration while the control normal feed.

The test population was tested five dogs in each group

hemp. Antioxidant Test

1. Test Method

Antioxidant activity was tested by the method of Kiharu, adding 2mg of linoleic acid to 1ml of distilled water, 10mg of Tween-20 to 1ml of distilled water, and 0.2M aerated for 1 hour. 1.0 ml of potassium phosphate (pH 7.4) was added to the crude herb extract obtained from Example to prepare a mixture. The mixture was reacted at 37 ° C. for 24 hours, taking 0.1 ml of the reaction solution at 3 hour intervals, adding 9.7 ml of 75% ethanol, 0.1 ml of 30% Ammonium thiocyanate and 0.02 M 3.5% of ferrous ammonium sulfate and 0.1 ml of hydrochloric acid were added and the absorbance was measured at 500 nm after 3 minutes.

2. Results

The antioxidant power measurement results of the herbal extracts obtained from the examples are shown in Table 6 and Table 7. As a control, the synthetic antioxidant BHT (dibutyl hydroxy toluen) and the natural antioxidant alpha-tocopherol (α-Tocopherol) Was used.

Table 7 shows the results of measuring the antioxidant power, Table 6 shows the results of the average value of the antioxidant power measured from Table 7. As shown in Table 6, the herbal extracts obtained from the examples showed higher antioxidant power than alpha-tocopherol and were slightly lower than BHT. In addition, the antioxidant power was measured using the herbal extract obtained in Example, and it was found that the control group had a high difference.

TABLE 6

0 hr 3 hrs 6 hrs 9 hrs 12 hrs 21 hrs 23 hrs 30 hrs Control 0.042 0.086 0.169 0.234 0.296 0.502 0.557 0.779 Herbal Extract 0.043 0.059 0.056 0.061 0.062 0.064 0.064 0.060 Tocopherol 0.042 0.094 0.127 0.137 0.144 0.277 0.346 0.546 BHT 0.041 0.044 0.043 0.045 0.043 0.046 0.046 0.045

TABLE 7

0 hr 3 hrs 6 hrs 9 hrs 12 hrs 21 hrs 23 hrs 30 hrs  Control 0.041 0.085 0.167 0.229 0.291 0.506 0.555 0.800 0.042 0.085 0.167 0.234 0.290 0.494 0.556 0.787 0.042 0.087 0.172 0.238 0.308 0.506 0.559 0.751 Average 0.042 0.086 0.169 0.234 0.296 0.502 0.557 0.779  Herbal Extract 0.043 0.058 0.056 0.059 0.062 0.065 0.062 0.060 0.041 0.059 0.057 0.062 0.062 0.063 0.063 0.059 0.045 0.061 0.056 0.062 0.061 0.063 0.063 0.059 Average 0.043 0.059 0.056 0.061 0.062 0.064 0.064 0.060  Tocopherol 0.041 0.092 0.129 0.139 0.145 0.276 0.352 0.537 0.044 0.099 0.126 0.137 0.144 0.278 0.343 0.552 0.041 0.092 0.127 0.135 0.144 0.276 0.344 0.548 Average 0.042 0.094 0.127 0.137 0.144 0.277 0.346 0.546  BHT (BHT) 0.041 0.045 0.042 0.047 0.043 0.045 0.045 0.045 0.041 0.043 0.043 0.045 0.044 0.047 0.046 0.045 0.040 0.045 0.043 0.045 0.043 0.045 0.048 0.046 Average 0.041 0.044 0.043 0.045 0.043 0.046 0.046 0.045

bar. Plasma Assay

Plasma was measured by total cholesterol measurement, blood fat measurement, lipid peroxidation content measurement. Three-week-old SD-rat was divided into groups of normal, nicotine and nicotine plus herbal extracts (n = 7) and sacrificed at 7 weeks of age.

Normal (n = 6): Free expression.

Nicotine (n = 8): Nicotine 4 mg / kg BW oral administration + 200 mg / kg BW physiological saline intraperitoneal administration.

Nicotine + herbal extract (n = 7): Nicotine 4 mg / kg BW oral administration + 200 mg / kg BW The herbal extract obtained from the Example is intraperitoneally administered.

1. Plasma Analysis

Blood was collected from the arteries of the sacrificed animals and treated with EDTA to prevent coagulation. Collected blood was centrifuged at 3,000 rpm for 10 minutes to separate serum and stored at -70 ℃ until analysis.

1) Total Cholesterol: Total absorbance was measured by measuring the absorbance at 505 nm of UV-VIS Spectrophotometer using blood total cholesterol measurement kit (Wako, Japan).

2) Triglyceride: Triglyceride in blood was measured using a blood triglyceride kit (Wako, Japan) and quantified by measuring absorbance at 410 nm of UV-VIS Spectrophotometer. The experimental results are shown in Table 8.

TABLE 8

Plasma (mg / dl) Normal Nicotine Nicotine + Herbal Extract Triglyceride 37.2 ± 6.0 a 77.1 ± 3.8 b 46.3 ± 2.7 a Total cholesterol 89.8 ± 3.2 c 68.0 ± 1.9 b 43.7 ± 3.0 a glucose 151.5 ± 2.4 a 174.4 ± 2.7 b 197.3 ± 3.5 c

2. Determination of Lipid Peroxidation Content (TBARS)

After administering nicotine and herbal extracts obtained from Examples for 4 weeks, the liver was extracted by anesthesia with diethyl ether. The extracted liver was stored at -70 ° C until analysis. 1 g of liver was homogenized with 100 mM trichloric acid (Tris-HCl), pH 7.4 buffer, and TBA color reagent was added to quantitatively analyze the TBARS content at 535 nm. The results are shown in Table 9. TBARS is Thiobarbituric Acid-Reactive Substance.

Lipid peroxidation was measured by measuring the lipid peroxidation product (MDA-MalonDiAldehyde) reacting with TBA reagent.

TABLE 9

TBARS (nmol / mg) Normal Nicotine Nicotine + Herbal Extract Liver 1.0 ± 0.0 b 0.91 ± 0.0 a 0.8 ± 0.1 a Brain 2.2 ± 0.1 2.0 ± 0.1 2.1 ± 0.1 Lung 1.5 ± 0.1 1.5 ± 0.1 1.5 ± 0.1 Plasma 0.3 ± 0.0 0.3 ± 0.0 0.3 ± 0.1

four. Test for increased immunity

1. Test Method

Spleens extracted from 3 week old ISR mice were washed with physiological saline to separate splenocytes from complete RPMI 1640 media. The isolated splenocytes were dispensed at 96 x wells at 2 x 105 cells / ml and incubated with stimulators such as Con A and LPS, respectively, to extract different concentrations of herbal medicines. After incubation for ₂ days at 37 ℃, 5% CO ₂ incubator (CO ₂ incubator) was treated with 20μ / well Almamar (alarmar blue) was measured for absorbance by a micro plate reader (micro plate reder).

* Herbal extract concentrations obtained from the examples: 20, 50, 100, 200 ug / ml

* All experiments were performed in a clean bench, and all samples including media were filtered (22 μm) and treated to cells.

Micro plate reder: Measurements were measured at 570 nm and reference 600 nm.

Alamar Blue assay: Alamar blue dye is a cytotoxicity assay using non-toxic metabolism by cell activity to turn blue. When the OD measured by a micro plate reder is low, the cell proliferation is evaluated to be reduced.

2. Results

After stimulating the control A (Control A) was added to the Alama blue (alamar blue) after 2 days of incubation, the results of the injection of the herbal extract obtained from the Example in the state of 32 hours has been shown in Table 10.

 TABLE 10

Average Standard error Normal -0.05 0.01 Control 0.09 0.01 Herbal Extract 200 0.14 0.01 Herbal Extract 100 0.13 0.01 Herbal Extract 50 0.11 0.01 Herbal Extract 25 0.08 0.01

Ah. Tests for Smoking-Related Biological Activities

Four weeks old asial mice (ICR mice) were used in the experiment after one week of adaptation. The animal breeding room was set to a room temperature of 23 ± 2 ° C., a humidity of 55 ± 10%, a ventilation number of 13 times / hour, and an illumination time of 12L / 12D hours / day (lighting up at 8 in the morning and turning off at 8 in the night). Feed and water were taken freely during the experiment.

Samples of the herbal extracts obtained from the examples were administered orally twice a day at 10 o'clock and 17 o'clock, and the daily dose of the sample measured the body weight of rats every week to determine the daily intake of human and average metabolic weight of human and rat ( The weight was calculated from the ratio of 3/4), and the average human weight was calculated as 65 kg.

1. Nicotine excretion test

The urine of mice was bred in metabolism cages. At 14 o'clock, nicotine dissolved at a concentration of 1 mg / ml in sterile distilled water for injection was administered at a dose of 10 mg per kg of body weight. The urine was collected at 14 o'clock the next day and nicotine concentration was measured by gas chromatography.

The pH was adjusted to 9 by adding dilute ammonia water to the urine. The resultant was maintained for 15 minutes on an Extreme column (manufactured by Merck), eluted with 15 ml of ethyl acetate, and eluted with distillation under reduced pressure. 100 µl of ethyl acetate was added to the residue to dissolve the sample.

2m in diameter packed with Gas chromatography (Hewlett Packard 5890, FID) and 2% Tharmon 1000 + 1% Potassium Hydroxide (KOH) Chromosorb WAM (8 / 100Mesh) A 3 mm glass column was used, the column temperature was 100 ° C., the carrier gas was nitrogen (N ₂ ), and the flow rate was performed at 60 ml / min.

2. Tar Detoxification Function Test

Tar dissolved at a concentration of 3 mg / ml in sterile distilled water for injection was intraperitoneally administered 30 mg per kg of body weight and the survival rate after 7 days was examined.

3. Plasma Total Oxidation Test

After oral administration of the herbal extracts obtained from the examples for 3 weeks, mice were anesthetized with ether and blood was collected from the heart to separate plasma from test tubes containing anticoagulants. Plasma total oxidative power was measured using a kit for measuring total antioxidant status of Randox (U.K.).

4. Statistical Processing

The measurements were analyzed by student's t-test and expressed in M ± SEM.

5. Results and Discussion

① Nicotine excretion test results

If you stop smoking, the nicotine accumulated in your body begins to decrease. During this period, it is very difficult to tolerate the body's desire for nicotine, which is addicted to nicotine. It is very important to get rid of nicotine accumulated in your body quickly in order to succeed in quitting smoking. It is known to be common to all addictive symptoms.

Table 11 examines the effect of ingestion of herbal extracts obtained from Examples on urinary excretion of nicotine in ICR mice.

 TABLE 11

Control Herbal Extract Urinary nicotine excretion (ug / day) excreted in the urine 0.217 ± 0.029 3.758 ± 0.523 ^*

( ¹ Values are mean ± SEM), n = 6.

* P <0.05 for the comparison with control group.

The urine nicotine excretion was increased by 17.3 times in the intake group of the herbal extract obtained from the example compared to the control group.

② Tar detox function test result

Table 12 examines the effects of ingestion of herbal extracts obtained from the examples on the toxicity of tar in ICR mice.

TABLE 12

Control Herbal Extract Survival ratio 1/10 7/10

¹ n = 10.

In the control group, one of ten tar-addicted rats survived, whereas in the administration group to which the herbal extracts obtained from the Example were administered, seven survived, and the herbal extracts obtained from the Example were treated by tar poisoning. Significantly reduced mortality. The above results show that the herbal extracts obtained from the examples effectively excrete nicotine and reduce the toxicity of tar. Therefore, herbal extracts obtained from the examples are supposed to act to enhance metabolism and physiological activity related to the removal of other toxic substances such as nicotine and tar accumulated in the body due to smoking.

Oxygen is an essential element for organisms doing organic hops, but active oxygen generated during incomplete combustion during energy metabolism destroys and destroys homeostasis of cells by denaturing and destroying macromolecules in cells.

Active oxygen is generated by factors such as tobacco and soot, and damages biological components such as proteins, DNA, enzymes, and T-cells to cause various diseases. Especially, unsaturated fatty acids, which are components of biological membranes, It is known to attack and produce lipid peroxide, causing aging and adult disease.

③ Plasma total oxidation test result

Table 13 shows the results of measuring total antioxidant status by separating plasma from the animal administered with the herbal extract obtained from the example. Table 13 compares the total oxidative powers of plasma in rats treated with functional foods for three weeks.

The total oxidative power of the group administered with the herbal extract obtained from the Example increased by 38.8% compared to the control group and there was a significant difference. (P <0.05) The antioxidant power of plasma may be considered as a representative value of the antioxidant power in the body, and the plasma Increasing the total oxidative power of means that the antioxidant power in the body was increased by administration of the herbal extract obtained from the example.

Green tea containing antioxidants of polyphenols such as catechin has also been reported to increase plasma antioxidant activity. These results show that the herbal extracts obtained from the examples can be used as additives to increase the biological antioxidant power and to protect the biomedical extracts from diseases caused by oxidative damage caused by factors such as smoking. .

TABLE 13

Relative Trolox ² concentration (n mol / ml plasma) Control 1.21 ± 0.05 Herbal extract obtained from the example 1.68 ± 0.08 *

( ¹ Values are mean ± SEM), n = 8.

( ² Water-soluble vitamin E analogue)

* P <0.05 for the comparison with control group.

I. Experiments on Dioxin Degradation in Blood

Experimental Method

☞ Subject: 4 applicants who have worked in incinerator in Hwaseong-gun, Gyeonggi-do for more than 10 years

☞ Dosage and administration period: 1 day (400mg × 3 tablets) 1.2g is administered after 4 weeks

☞ Method: US EPA methods & Canada IOS methods

☞ Analyzer: HRGC / HRMS (Jeol, JMS-700T)

☞ Analysis period: Jan. 22, 2003 ~ Feb. 7, 2003

1. Increase or decrease of dioxin concentration

(1) Total concentration (n = 4)

 (Mean; 548.4 pg / g lipid, range; 234.9 to 671.5 pg / g lipid)

→ All concentrations of dioxins ranging from 4-chloride to 8-chloride are reduced in all three patients with pre-dose data (42.0% decrease on average)

(2) TEQ concentration (n = 4)

 (Mean; 52.1 pg I-TEQ / g lipid, range; 7.5 to 74 pg I-TEQ / g lipid)

¡Æ when evaluating the concentrations of 17 toxic dioxins, all three data with pre-dose data are reduced, similar to the total concentration.

Table 14 Concentration of Dioxin in Blood Before and After

Sample Name Dioxin Concentration bl-0025 bl-0026 bl-0028 Before taking After taking % Reduction Before taking After taking % Reduction Before taking After taking % Reduction total PCDD 438.7 228.0 48.0 336.7 203.0 39.7 225.8 157.4 30.3 total PCDF 745.7 443.6 40.5 606.1 436.0 28.0 249.3 77.5 68.9 total PCD / Fs 1184.4 671.5 43.3 942.9 639.0 32.2 475.1 234.9 50.6 TEQ PCDD / Fs 68.7 61.7 10.2 78.7 65.0 17.3 13.0 7.4 42.7

* Dioxin concentration unit: pg / g lipid, TEQ concentration unit: pg I-TEQ / g lipid

* bl-0027 sample was excluded from the comparison table because there is no data before taking it.

2. Comparison of total concentration by congener (see Table 1)

(1) dioxins

-In case of HpCDD and OCDD, all three are reduced.

Low chloride dioxin was not detected as before.

(2) furans

-All three had reduced or no detectable high chloride furans (HxCDF, HpCDF, OCDF).

However, all three TCDFs that were not detected before are detected in trace amounts.

3. Conclusion

 Total concentrations, including non-toxic dioxins, indicate a significant decrease in blood levels (average 42.0%), and a decrease of 23.4% in the congener-specific concentrations of the 17 toxic dioxins (TEQ). . In general, high chloride was identified.

 This test requires a systematic and long-term monitoring for a more accurate evaluation because the results of experiments comparing the concentration of dioxins in the blood before and after 1 month of taking the nutrient may affect the diet.

According to the above-described herbal extract for removing harmful components accumulated in the human body according to the present invention and a method for manufacturing the same, as shown from the above experimental results, when the herbal extract of the present invention is administered to the human body and nicotine is administered at the same time It has the effect of lowering the amount of nicotine in the blood and the action of releasing nicotine in the urine. In addition, exposure to carbon monoxide has the effect of lowering the concentration of CO-hemoglobin in the blood. In addition, high antioxidant power and total cholesterol, blood fat, lipid peroxidation effect is shown to increase the body's immunity and has the advantage to increase the immune system against disease.

Herbal extract of the present invention, including nicotine in the blood can be expected to prevent the various effects that can occur due to smoking by removing harmful components in the human body that may occur due to smoking.

In addition, the herbal extract of the present invention can be easily taken in addition to the functional food, it provides an advantage that can be prepared and supplied in various forms of preparation.

Claims (8)

A first step of drying the dandelion, marigold, black yam, green tea leaf, weak wheat, honeysuckle, licorice, chajeoncho, samchil and purslane respectively to form a dry matter; A second step of forming an extract solution in which the solvent is added to each dried product of the first step and heated to extract the components of the dried product into the solvent; A third step of removing the solid material by filtering the extraction solution of the second step; A fourth step of obtaining each extract by evaporating the solvent component contained in the filtered extract solution of the third step; And The fifth step of providing a mixed extract by mixing the extract of the fourth step in a predetermined ratio; Herb extract manufacturing method for removing harmful components accumulated in the human body comprising a. Pogongyoung, marigold, black yam, green tea leaf, weak wheat, honeysuckle, licorice, chajeoncho, samchil and purslane each of the first step of drying; A second step of mixing the dry matter of the first step at a predetermined ratio to provide a mixed dry matter; A third step of adding a water-soluble solvent to the mixed dry matter of the second step to form a mixed extractive solution in which the dry ingredient is extracted into the solvent; A fourth step of removing the solid material by filtering the mixed extraction solution of the third step; And a fifth step of providing a mixed extract by evaporating a solvent component contained in the mixed extract solution filtered in the fourth step. The method according to claim 1 or 2, The mixing ratio of each extract and each dried product is poongyoung 5 ~ 40% by weight, marigold 0.5 ~ 10% by weight, 5 ~ 20% by weight in black, green tea leaves 5 ~ 20% by weight, chajeoncho 5 ~ 40% by weight, weak wheat 5 ~ 40 Weight%, honeysuckle 5-30% by weight, licorice 5-20% by weight, samchil 0.5-15% by weight, purslane 5-20% by weight, the method for producing a herb extract for the accumulation of harmful components in the human body. The method according to claim 1 or 2, Wherein the solvent is water, lower alcohols, esters, ketones, aliphatic hydrocarbons, chloroform one or more selected from the group consisting of chloroform accumulation method for extracting herbal components in the human body. The method according to claim 1 or 2, Evaporating the solvent component is a method for producing a herb extract for removing harmful components accumulated in the human body, characterized in that it comprises a step of heating to a temperature of 40 to 60 ℃ at 0.005 to 0.1 atm. The method according to claim 1 or 2, Mixing the herbal extract in a ratio of 5 to 70% by weight relative to the conventional food ingredients to provide a functional food, characterized in that it further comprises the step of providing a herbal food for the removal of harmful components accumulated in the human body. The method according to claim 1 or 2, Mixing the herbal extract with an excipient comprising lactose, corn starch, cellulose, magnesium stearate to remove the harmful components accumulated in the human body, characterized in that it further comprises the step of preparing in the form of powder, granules, tablets or capsules. Herbal extract preparation method. Pogongyoung 5-40% by weight, marigold 0.5-10% by weight, 5-20% by weight in yam, green tea leaves 5-20% by weight, Chajeoncho 5-40% by weight, weak wheat 5-40% by weight, honeysuckle 5-30% by weight Herb extract for removing harmful components accumulated in the human body, characterized in that 5 to 20% by weight, licorice 5 to 15% by weight, and 5 to 20% by weight purslane.