KR20070107715A - 변형된 아실트랜스퍼라제 폴리펩티드 및 관련폴리뉴클레오티드 - Google Patents

변형된 아실트랜스퍼라제 폴리펩티드 및 관련폴리뉴클레오티드 Download PDF

Info

Publication number
KR20070107715A
KR20070107715A KR1020077019278A KR20077019278A KR20070107715A KR 20070107715 A KR20070107715 A KR 20070107715A KR 1020077019278 A KR1020077019278 A KR 1020077019278A KR 20077019278 A KR20077019278 A KR 20077019278A KR 20070107715 A KR20070107715 A KR 20070107715A
Authority
KR
South Korea
Prior art keywords
gly
ala
glu
leu
arg
Prior art date
Application number
KR1020077019278A
Other languages
English (en)
Korean (ko)
Inventor
로엘로프 아리 랜스 보벤베르그
앵크 크레베르
안토니 콕스
라 칭 샤를렌 토
매튜 토빈
스테판 제이 젠느
용 홍 첸
Original Assignee
디에스엠 아이피 어셋츠 비.브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 디에스엠 아이피 어셋츠 비.브이. filed Critical 디에스엠 아이피 어셋츠 비.브이.
Publication of KR20070107715A publication Critical patent/KR20070107715A/ko

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01164Isopenicillin-N N-acyltransferase (2.3.1.164)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020077019278A 2005-02-24 2006-02-24 변형된 아실트랜스퍼라제 폴리펩티드 및 관련폴리뉴클레오티드 KR20070107715A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05101423 2005-02-24
EP05101423.1 2005-02-24

Publications (1)

Publication Number Publication Date
KR20070107715A true KR20070107715A (ko) 2007-11-07

Family

ID=34938811

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020077019278A KR20070107715A (ko) 2005-02-24 2006-02-24 변형된 아실트랜스퍼라제 폴리펩티드 및 관련폴리뉴클레오티드

Country Status (5)

Country Link
EP (1) EP1851309A2 (fr)
KR (1) KR20070107715A (fr)
CN (1) CN101142312A (fr)
BR (1) BRPI0609248A2 (fr)
WO (1) WO2006089946A2 (fr)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE105860T1 (de) * 1988-04-08 1994-06-15 Biochemie Gmbh Gen und genprodukt zur herstellung von beta- lactamverbindungen.
FI104984B (fi) * 1988-08-11 2000-05-15 Dsm Nv Menetelmä biosynteettisten tai säätelygeenien identifioimiseksi ja käyttämiseksi sekundääristen metaboliittien tuoton parantamiseksi

Also Published As

Publication number Publication date
BRPI0609248A2 (pt) 2010-03-09
WO2006089946A2 (fr) 2006-08-31
WO2006089946A3 (fr) 2007-06-21
EP1851309A2 (fr) 2007-11-07
CN101142312A (zh) 2008-03-12

Similar Documents

Publication Publication Date Title
CN1331751A (zh) 改进的头孢菌素的体内生产方法
SK28894A3 (en) Bioprocess for preparing 7-adca
AT408660B (de) Nukleinsäure-molekül, das für cephalosporin-acetylesterase kodiert
AU5248698A (en) Mutant penicillin g acylases
KR101985911B1 (ko) Achromobacter sp. CCM 4824 유래 페니실린 G 아실라제 변이체 및 이의 이용
EP1799817B1 (fr) Expandases mutantes
US6905849B1 (en) Process for preparing clavam derivatives by using polypeptides having β-lactam synthetase activity
RU2388826C2 (ru) РЕКОМБИНАНТНАЯ ДНК, КОДИРУЮЩАЯ ФУНКЦИОНАЛЬНО АКТИВНЫЙ ГИБРИДНЫЙ БЕЛОК G17ACA-АЦИЛАЗЫ С ХИТИН-СВЯЗЫВАЮЩИМ ДОМЕНОМ (BrdG17ACA-cbd), РЕКОМБИНАНТНАЯ ПЛАЗМИДА pSVH0108, ОБЕСПЕЧИВАЮЩАЯ ЕГО СИНТЕЗ В КЛЕТКАХ Escherichia coli, И РЕКОМБИНАНТНЫЙ ШТАММ Escherichia coli BL21(DE3)/pSVH0108-ПРОДУЦЕНТ BrdG17ACA-cbd
KR20070107715A (ko) 변형된 아실트랜스퍼라제 폴리펩티드 및 관련폴리뉴클레오티드
US5328839A (en) Oxido reductase enzyme system obtained from P. chrysogenum
CN104364376B (zh) 突变的头孢菌素羟化酶及其在脱乙酰基头孢菌烷酸合成中的应用
EP1383886B1 (fr) D-hydantoinase d'ochrobactrum anthropi
DK175769B1 (da) DNA-sekvens i renset og isoleret form, der koder for enzymet penicillinacetyltransferase, protein, der koder for DNA-sekvensen, vektor, ...........
WO2008107782A2 (fr) Hydroxylase modifiée et ses applications
WO2007023369A1 (fr) Expandase-hydroxylase modifiee et applications correspondantes
AU2002254519A1 (en) D-hydantoinase from ochrobactrum anthropi
EP1675946A1 (fr) Methode de production de penicilline
KR20080028924A (ko) 변이주 익스팬다제 및 베타-락탐 화합물의 생성에 있어서이들의 용도
WO2009013611A2 (fr) Estérase modifiée et ses applications
RU2300566C2 (ru) РЕКОМБИНАНТНАЯ ПЛАЗМИДА pSVH0106, ОБЕСПЕЧИВАЮЩАЯ СИНТЕЗ Gl7ACA-АЦИЛАЗЫ В КЛЕТКАХ Escherichia coli, И РЕКОМБИНАНТНЫЙ ШТАММ Escherichia coli BL21(DE3)/pSVH0106-ПРОДУЦЕНТ Gl7ACA-АЦИЛАЗЫ
EP0444758A1 (fr) ProcédÀ© pour la modification d'ACV-synthètase et utilisation de l'enzyme modifié pour la production d'antibiotiques bêta-lactames
US20030143677A1 (en) Activated rec-D-hydantoinases
Kovacevic et al. Genetic Manipulation of the ß-lactam Antibiotic Biosynthetic Pathway Paul L. Skatrud, JoAnn Hoskins, John S. Wood, Matthew B. Tobin, James R. Miller
JPH05192162A (ja) Dnaおよびその用途
JPH06292586A (ja) シスタチオニンγ−リアーゼ遺伝子

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid