KR20070107715A - Altered acyltransferase polypeptides and related polynucleotides - Google Patents

Abstract

The present invention relates to novel acyltransferase polypeptides, as well as the polynucleotides that encode them, that are particularly useful in the production of beta-lactam intermediates and antibiotic compounds having adipoyl side chains. The present invention is also directed to related vectors, host cells, and methods for making and using the novel acyltransferase polypeptides.

Description

ALTERED ACYLTRANSFERASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES}

The present invention relates generally to novel acyltransferase polypeptides useful for the preparation of beta-lactam intermediates and antibiotic compounds.

The most important classes of beta-lactam antibiotics are penicillin (phenam) and cephalosporins (seph-3-em). Penicillin is produced only by fibrous fungi ( Penicillium chrysogenum or Aspergillus nidulans ), while cephalosporins are produced by fibrous fungi (Acremonium chrysogenum ). In addition, it is produced by bacteria (eg, Streptomyces clavuligerus ). All beta-lactam antibiotics share common structural features of the 4-membered beta-lactam ring. Natural penam and cef-3-M are synthesized from the same three amino acid precursors: L-alpha-aminoadipic acid (L-alpha-AAA), L-cysteine and L-valine. The first two steps of the biosynthetic pathway of penicillin and cephalosporin are identical. In a first step, an amino acid precursor is condensed to tripeptide delta-L-alpha-aminoadifil-L-cysteinyl-D-valine (ACV). Essential reaction cycles (eg, recognition, activation and production of peptide bonds) are facilitated by the multifunctional enzyme ACV synthetase (ACVS). In the second step, the resulting linear tripeptide is cyclized by oxidative ring closure to produce a bicyclic ring structure, ie a 4-membered beta-lactam ring fused to a 5-membered thiazolidine ring characteristic of all penams. . Cyclization is promoted by the enzyme isopenicillin N synthase (IPNS). The resulting compound represents the first bioactive intermediate and is called isopenicillin N (IPN) (see generally EP 0 422 790 (Miller et al.)).

After IPN synthesis, the biosynthetic pathways leading to the production of penicillin and cephalosporin are divided. In some fungi, such as penicillium chrysogenum and Aspergillus nidulans , the hydrophilic L-alpha-AAA side chain of IPN may be substituted with a hydrophobic acyl group in the third and final exchange steps. The side chains can be derived intracellularly (eg, hexonic acid or octenoic acid) or supplied externally. The only direct fermented penicillins of industrial importance are penicillin V and penicillin G produced by addition of exogenous side chain precursor phenoxyacetic acid or phenylacetic acid, respectively. Exchange is facilitated by acyl coenzyme A (CoA): isophenicillin N acyltransferase (AT), which is encoded by the penDE gene.

In contrast, cef-3-M isomerized by the L-alpha-AAA side chain of IPN to D enantiomers by IPN epimerase to produce penicillin N, a precursor of antibiotics containing the cef-3-M nucleus. Are manufactured. For example, in fungal acremonium chrysogenum , the alpha- aminoadipic acid side chain of IPN isomerizes to produce penicillin N, and then the penicillin 5-membered thiazolidine ring is deacetoxycephalosporin synthetase. It is "extended" by the DAOCS expandase activity to produce deacetoxycephalosporin C (DAOC) containing a 6-membered dihydrothiazine ring that is characteristic of cephalosporins.

Most of the so-called natural beta-lactams (e.g., penicillin F, isopenicillin N, cephalosporin C, etc.) are antibiotics because they are unstable, difficult to purify from fermentation broth and have only limited antibiotic effects and / or are produced in low yield Has limited utility as a substance. Substitution of the side chains of these beta-lactams with other side chains results in semisynthetic penicillins and cephalosporins such as amoxicillin, ampicillin and cephalexin, which are more stable, easier to separate and have higher antibiotic activity.

The wide variety of side chains found in commercially meaningful beta-lactam compounds has become increasingly important in achieving more economical and effective methods of preparing key intermediates for the synthesis of various beta-lactam compounds.

Cephalosporin Intermediates 7-ADCA is an important intermediate for the synthesis of many semisynthetic cephalosporins. The intermediate is currently produced by chemical derivatization of penicillin G or by bioprocess as described in EP 0532341.

In EP 0532341 it is shown that adiphyl -7-ADCA is produced by a penicillium chrysogenum strain modified to express an expandase and provides a side chain precursor adipic acid. The adifil side chain is then removed with an appropriate enzyme to produce 7-ADCA. Thus, the AT enzyme can accept side chains other than phenyl- or phenoxyacetic acid, but can improve the AT enzyme's ability to accept side chains other than phenyl- or phenoxyacetic acid or modify its substrate specificity. It is unpredictable.

Summary of the Invention

The present invention provides a polypeptide having acyltransferase activity under modified substrate specificity compared to native penicillium chrysogenum acyltransferase. In particular, the present invention provides acyltransferase polypeptides having an improved ability to produce adipoyl-6-APA from alpha-aminoadipoyl-6-APA compared to native penicillium chrysogenum acyltransferase. More particularly, the improved capacity is at least 1.5 times, preferably at least 2 times, more preferably at least 5 times, even more preferably at least 10 times, most preferably at least natural penicillium chrysogenum acyltransferase activity. More than 30 times.

In another aspect, the invention relates to an isolated polypeptide having acyltransferase activity, selected from the group consisting of:

(a) a polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38;

(b) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42;

(c) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52;

(d) has an amino acid sequence of 96% or more identical to SEQ ID NO: 54, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, and position 277 A polypeptide having one or more residues selected from the group consisting of Gly, Glu at position 291 and Gly at position 299; And

(e) A polypeptide encoded by a polynucleotide that hybridizes under stringent conditions on a substantially full length nucleotide sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 42 and SEQ ID NO: 52.

In another aspect, the present invention relates to a polynucleotide encoding an acyltransferase polypeptide.

In another aspect, the present invention relates to expression vectors, host cells, and methods useful for producing beta-lactam compounds using the acyltransferase polypeptides of the present invention.

In a first aspect, the present invention provides novel polypeptides having acyltransferase ("AT") activity, selected from the group consisting of the following polypeptides.

In another aspect, the invention relates to an isolated polypeptide having acyltransferase activity, selected from the group consisting of:

(a) a polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38;

(b) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42;

(c) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52;

(d) has an amino acid sequence of 96% or more identical to SEQ ID NO: 54, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, and position 277 A polypeptide having one or more residues selected from the group consisting of Gly, Glu at position 291 and Gly at position 299; And

(e) A polypeptide encoded by a polynucleotide that hybridizes under stringent conditions on a substantially full length nucleotide sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 42 and SEQ ID NO: 52.

Acyltransferase polypeptides of the invention are useful for the production of beta-lactam compounds, such as, for example, penicillin antibiotic compounds with adipoyl side chains. As used herein, the terms “acyltransferase polypeptide”, “AT polypeptide”, “acyltransferase enzyme” and “AT enzyme” are used interchangeably to refer to polypeptides exhibiting acyltransferase activity herein. do. The term “acyltransferase activity” is used herein to detect at least 6-aminophenylanic acid (“6-APA”) and adipoyl-coenzyme A (“Ad-CoA”) using the assay described in Example 3. It is used to indicate the ability of an enzyme to promote a reaction that converts a possible amount of adipoyl-6-aminophenicylic acid ("Ad-6-APA"). Ad-6-APA is a useful intermediate that can be converted into penicillin antibiotics with adipoyl side chains or the 6-APA nucleus. Alternatively, ad-6-APA is a useful intermediate that can be extended to adipoyl-7-ADCA, for example as described in EP 0 532 341. AT enzymes can also promote hydrolysis of IPN, where alpha-AAA side chains are separated to produce 6-APA. The terms "acyltransferase polynucleotide" and "AT polynucleotide" are used interchangeably herein to refer to a polynucleotide encoding an acyltransferase polypeptide. It should be noted that reference to adifoil should not be considered limiting, but only in the sense of characterizing one or more catalytic capacities of an enzyme.

As used herein, the term “isolated” refers to a polynucleotide or polypeptide that is substantially free of other natural cell materials and culture media since it is substantially free of other materials commonly found together in nature. For example, an “isolated polynucleotide” has few sequences adjacent to the polynucleotide at its native genomic location.

The terms "polynucleotide", "nucleic acid" and "nucleic acid molecule" are used interchangeably herein to refer to DNA, RNA or synthetic analogs thereof. As used herein, the terms "polypeptide" and "protein" are used interchangeably herein to refer to a polymer of amino acids.

The terms "percent identity" and "% identity" are used herein to count the same number of identical pairs during alignment, divide the same number of pairs by the length of the reference sequence, and use a basic cluster W parameter below to determine a slow / correct pair. Interchangeably to refer to the percent amino acid sequence identity obtained by Cluster W analysis (version W 1.8 available from the European Bioinformatics Institute, Cambridge, UK) to achieve alignment. Used: gap opening penalty: 10; Interval extension penalty: 0.10; Protein weighting matrix: Gonnet series; DNA weighting matrix: IUB; Toggle Slow / Fast Pair Sort = Slow or Full Sort.

The invention also provides an acyltransferase polypeptide having an amino acid sequence that is at least 97% identical to SEQ ID NO: 38, typically at least 98% identical to SEQ ID NO: 38, and in some embodiments at least 99% identical to SEQ ID NO: 38. to provide. In another aspect, the present invention provides an acyltransferase polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 52. The present invention includes an AT polypeptide having an amino acid sequence of at least 97% identical to SEQ ID NO: 52 and an amino acid sequence of at least 98% and at least 99% identical to SEQ ID NO: 52.

In another aspect, the invention is at least 96% identical to wild-type acyltransferase (SEQ ID NO: 54) from Penicillium chrysogenum , wherein Arg at position 97, Val at position 221, 251 An acyltransfer having an amino acid sequence also having one or more residues selected from the group consisting of Pro at position 254, Glu at position 254, Ile at position 259, Gly at position 277, Glu at position 291 and Gly at position 299 It provides a lase polypeptide. In certain embodiments, the amino acid sequence of an AT polypeptide may comprise two or more residues selected from the group, and sometimes four or more residues, often up to about six residues (eg, Pro at position 251, Glu at position 254, Ile at position 259, Gly at position 277, Glu at position 291, and Gly at position 299. The amino acid position referred to herein is the position corresponding to SEQ ID NO: 54 in the optimal alignment of the AT polypeptide sequence having SEQ ID NO: 54.

The term "optimally ordered" refers to an alignment generated using the Cluster W algorithm ( Nucleic Acid Research , 22 (22): 4673-4680, 1994). For amino acid sequences that are optimally aligned with a reference sequence, the amino acid residues "correspond to" positions in the reference sequence that residues pair with in the alignment.

In another aspect of the invention, the acyltransferase polypeptide of the invention has a higher acyltransferase activity as determined by the assay described in Example 3 than the wild type penicillium chrysogenum acyltransferase (SEQ ID NO: 54). Is provided. Certain acyltransferase polypeptides of the invention exhibit an acyltransferase activity that is at least about 1.6-fold greater than wild type penicillium chrysogenum acyltransferase (SEQ ID NO: 54). In certain embodiments, the acyltransferase polypeptides of the invention are at least about 2 times, sometimes at least about 5 times, in some embodiments at least about 10 or 20 times at least about 30 or 50 times than wild-type penicillium chrysogenum acyltransferase Up to higher acyltransferase activity.

In another aspect, the invention provides an acyltransferase encoded by a polynucleotide that hybridizes under stringent conditions on substantially full length nucleotides, encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 52 Provide a polypeptide.

As used herein, the term “stringent hybridization and / or wash conditions” in connection with nucleic acid hybridization experiments such as Southern and Northern hybridizations is sequence dependent and differs under different environmental parameters. Extensive guidance on hybridization of nucleic acids is given in Tijessen, "Laboratory Techniques in Biochemistry and Molecular Biology-Hybridization with Nucleic Acid Probes", Part I, Chapter 2 (Elsevier, New York), 1993.

In the present invention, “very stringent” hybridization and / or wash conditions are generally selected to be about 5 ° C. or less below the thermal melting point (T _m ) for a particular sequence at defined ionic strength and pH (as mentioned below). Very stringent conditions may also be referred to in comparative terms). T _m is the temperature (under defined ionic strength and pH) at which 50% of the test sequence hybridizes to a fully matched probe. Very stringent conditions are chosen to be equal to the T _m for the particular probe.

Stringent and very stringent hybridization and wash conditions can be readily determined experimentally for any nucleic acid. For example, in determining very stringent hybridization and washing conditions, the hybridization and washing conditions are gradually increased until a selected set of criteria is met (eg, during hybridization or washing, the temperature is increased and , By decreasing salt concentration, increasing detergent concentration and / or increasing concentration of organic solvent such as formamide).

An example of stringent hybridization conditions for hybridization of complementary nucleic acids with more than 100 complementary residues on a filter in a Southern or Northern blot uses 50% formamide with 1 mg of heparin at 42 ° C. and hybridization overnight To do. An example of stringent washing conditions is a 0.2 × SSC wash for 15 minutes at 65 ° C. (Sambrook et al., “Molecular Cloning-A Laboratory Manual”, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, for a description of SSC buffers). , 1989). Often very stringent washes are preceded by less stringent washes to remove background probe signals. An example of a less stringent wash is a 2 × SSC wash at 40 ° C. for 15 minutes.

Certain acyltransferase polypeptides of the invention include SEQ ID NOs: 2 (H1), 4 (H2), 6 (H3), 8 (H4), 10 (H5), 12 (H6), 14 (H7), 16 (H14). ), 18 (H25), 20 (H26), 22 (H27), 24 (H28), 26 (H29), 28 (H30), 30 (H31), 32 (H32), 34 (H33), 36 (H34 A polypeptide having an amino acid sequence corresponding to 38 (H35), 40 (H36), 42 (H37), 44 (H38), 46 (H40), 48 (H41), 50 (H42) and 52 (H43). Include. Acyltransferase activity against the polypeptide is described in Example 3. The invention also provides an acyltransferase polypeptide that is a variant of the polypeptide having substitutions, deletions and / or insertions of 1 to 6 amino acid residues.

As used herein, the term “variant” refers to a sequence having a high percentage of identity with respect to the reference sequence. Variant acyltransferase polypeptides of the invention may be natural or non-natural products. Variant acyltransferase polypeptides may have substitutions, deletions and / or insertions of one to six amino acid residues. Such substitutions, deletions and / or insertions may occur at one or more sites in the polypeptide and may occur at one or more sites within the polypeptide as well as at the N-terminus and / or C-terminus of the polypeptide. Variant polypeptides included in the present invention have acyltransferase activity.

Conservative variants include basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (glycine, alanine, serine , Threonine, proline, cysteine and methionine).

Variants of the acyltransferase polypeptides of the invention can also be produced using mutagenesis and directed evolution methods known to those of ordinary skill in the art. Libraries prepared by the above methods can be searched for variants with acyltransferase activity as measured using the assay described in Example 3.

In another aspect, the invention relates to a fragment of an AT polypeptide of the invention that exhibits AT activity in the assay described in Example 3. Fragments can be readily prepared using commercially available exonucleases and endonucleases according to known methods. As used herein, the term “fragment” refers to a polypeptide having a deletion of 1 to 15 amino acid residues from one or both of the carboxy and / or amino termini. In certain embodiments, the deletions are 1 to 10 amino acid residues, in some cases 1 to 5 amino acid residues. AT fragments of the invention have an AT activity that is at least 1.6 times greater than wild-type penicillium chrysogenum AT activity using the assay described in Example 3. In some aspects , the AT fragment of the invention has at least two times, typically up to about 30 times, AT activity of the wild-type penicillium chrysogenum AT activity in the assay of Example 3.

In a second aspect, the invention provides a polynucleotide encoding the acyltransferase polypeptide of the invention. In one embodiment, the invention provides an isolated polynucleotide encoding a polypeptide having acyltransferase activity, selected from the group consisting of:

(a) a polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38;

(b) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42;

(c) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52;

(d) has an amino acid sequence of 96% or more identical to SEQ ID NO: 54, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, and position 277 A polypeptide having one or more residues selected from the group consisting of Gly, Glu at position 291 and Gly at position 299; And

(e) A polypeptide encoded by a polynucleotide that hybridizes under stringent conditions on a substantially full length nucleotide sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 42 and SEQ ID NO: 52.

Certain acyltransferase polynucleotides of the invention include SEQ ID NOs: 1 (H1), 3 (H2), 5 (H3), 7 (H4), 9 (H5), 11 (H6), 13 (H7), 15 ( H14), 17 (H25), 19 (H26), 21 (H27), 23 (H28), 25 (H29), 27 (H30), 29 (H31), 31 (H32), 33 (H33), 35 ( With polynucleotide sequences corresponding to H34), 37 (H35), 39 (H36), 41 (H37), 43 (H38), 45 (H40), 47 (H41), 50 (H41) and 51 (H43) Polynucleotides. SEQ ID NOs: 13 and 15 are polynucleotide variants encoding the same acyltransferase polypeptide (ie, SEQ ID NOs: 14 and 16 are identical). One of ordinary skill in the art will readily recognize that due to the degeneracy of the genetic code, there are many nucleotide sequences encoding the acyltransferase polypeptides of the invention and can be readily determined using codon tables.

One of ordinary skill in the art will modify each codon in the nucleic acid (except ATG, which is usually the only codon for methionine, and TGG, which is typically the only codon for tryptophan) to encode a functionally identical polypeptide by standard techniques. It will be appreciated. Thus, each latent variation of nucleic acid encoding a polypeptide is inherent in any described sequence. Acyltransferase polynucleotides can be codons optimized for expression in a particular host organism by modifying the polynucleotide to conform to the optimal codon usage of the desired host organism. Those skilled in the art will appreciate that tables and other references that provide selection information for a wide variety of organisms are readily available.

Polynucleotides of the present invention can be prepared using methods known in the art. See, eg, Carruthers, et al., Cold Spring Harbor Symp. Quant. Biol ., 47 : 411-418, 1982; and Adams, et al., J. Am. Chem. Soc ., 105 : 661, 1983. Typically, oligonucleotides of up to about 100 bases are synthesized individually and then conjugated to each other enzymatically or chemically to produce the desired sequence.

In a third aspect, the present invention provides an expression cassette comprising an acyltransferase polynucleotide of the present invention operatively linked to a regulatory sequence, such as a promoter. Expression cassettes of the present invention provide for gene delivery of acyltransferase polynucleotides and expression and production of acyltransferase polypeptides they encode. The regulatory sequences of the construct act to induce transcription and translation of the acyltransferase polynucleotides.

As used herein, the term “structure” refers herein to single- or double-stranded nucleic acid molecules consisting of DNA, RNA, synthetic analogs thereof, or mixtures of two or more thereof. In addition to the expression cassette, the nucleic acid construct may or may not contain other nucleic acid fragments that can provide other functions useful for gene delivery and / or expression, such as selective marker genes.

Nucleic acid constructs can be derived from many different sources, including bacteria, fungi and plants. Suitable fungal sources include Aspergillus and Penicillium (eg, Penicillium chrysogenum ). The nucleic acid construct may be in the form of a vector suitable for engineering, transformation and / or expression of the acyltransferase polynucleotides of the invention. For example, nucleic acid constructs may be selected and / or designed according to the characteristics of the host organism in which they are to be introduced. Thus, the constructs can be replicated independently, ie the constructs can typically be present in extrachromosomal presence, for example in plasmids. In another aspect, the nucleic acid construct can be a molecule that is integrated into the genome when introduced into a host cell.

Suitable regulatory sequences used in the practice of the present invention may be any polynucleotide that exhibits the appropriate activity in the host cell of interest, and may be natural or synthetic in origin. Suitable promoters for use in fibrous fungal host cells include, for example, the Aspergillus nidulan trpC promoter [Yelton, MM, et al., Proc. Natl. Acad. Sci. USA , 81 : 1470-1474, 1984; Mullaney, EJ, et al., Mol. Gen. Genet ., 199 : 37-45, 1985], Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter [Punt, PJ et al., Gene , 56 (1): 117-124 , 1987; Punt, PJ et al., Gene , 69 (1): 49-57, 1988]. Aspergillus nidulans ADH3 promoter (Mcknight et al., EMBO J. , 4 : 2093-2099, 1985), Aspergillus awamori glucoamylase gene promoter [Nunberg, JH, et al., Mol. Cell Biol ., 4 (11): 2306-2315, 1984], and A. oryzae transcription extension factor gene promoter [Kitamoto, N., et al., Appl. Microbiol. Biotechnol ., 50 (1): 85-92, 1998]. Other suitable promoters include those of the penicillin biosynthetic pathway itself, such as the penicillium chrysogenum pcbC (IPNS) promoter [Barredo, JL, et al., Mol. Gen. Genet ., 216 (1): 91-98, 1989], or penicillium chrysogenum penDE (AT) promoter [Diez, B., et al., Mol. Gen. Genet ., 218 (3): 572-576, 1989). If one or more polypeptides are to be prepared, it may be advantageous to use the same or similar promoters to control the simultaneous production of the polypeptides. For example, when it is desired to transform host cells with multiple polynucleotides encoding enzymes used in the beta-lactam antibiotic biosynthetic pathway, it is desirable to simultaneously produce intermediates in the pathway to improve production efficiency. can do.

Nucleic acid constructs of the invention may also comprise polynucleotides encoding selectable markers, eg, markers that facilitate the selection of cells containing nucleic acid constructs. Those skilled in the art will recognize that many selectable markers are suitable for use in the expression vectors of the present invention. Suitable selectivity markers include kanamycin resistance gene, chloramphenicol resistance gene, and other antibiotic resistance- conferring genes. Preferred selectable markers for use in fibrous fungi are acetamides [Corrick, CM, et al., Gene , 53 (1): 63-71, 1987], hygromycin resistance [Kaster, KR, et al. , Nucleic Acids Res ., 11 (19): 6895-6911, 1983; Punt, PJ, et al., Gene , 56 (1): 11724, 1987], pleomycin resistance [Drocourt, D., et al., Nucleic Acids Res ., 18 (13): 4009, 1990], benomil Resistance [Orbach, MJ, et al., Mol. Cell. Biol ., 6 (7): 2452-2461, 1986]. Other genes useful as selectivity markers include factors for nutritional complementarity, such as PyrG [Cantoral, JM, et al., Nucleic Acids Res ., 16 (16): 8177, 1988], ArgB [Upshall, A. , et al., Mol. Gen. Genet ., 204 (2): 349-354, 1986], nia D [Johnstone, IL, et al., Gene , 90 (2): 181-192, 1990] and TrpC [Penalva, MA, Nucleic Acids Res ., 15 (4): 1874, 1987]. In penicillium species, nucleic acid constructs containing ampicillin resistance genes are less useful when beta-lactam production is the response because they encode beta-lactamases that will degrade beta-lactam when the gene is expressed. . Instead, markers such as pleomycin resistance gene (phlR) can be used in penicillium species if beta-lactam production is the response.

Nucleic acid constructs of the invention may also contain a target signal or secretory signal sequence to induce the expression of an acyltransferase polypeptide at a desired position in a host cell or in fermentation medium. The target signal and / or secretory signal sequence is linked to an acyltransferase polynucleotide such that both are in the same reading frame. The signal sequence may be natural to the host cell or may have synthetic or foreign origin. If the host organism is a fibrous fungus, the signal peptide may be derived from fungal enzymes, such as amylase of the genus Aspergillus . Procedures used to produce constructs with the desired properties, including manipulation of various nucleotide fragments, alignment of reading frames, and the like, are known to those skilled in the art (eg, Sambrook et al. , "Molecular Cloning: A Laboratory Manual", 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989).

The host cell into which the construct is introduced can be any cell having the desired properties. For example, it may be a cell capable of producing a large amount of the acyltransferase polypeptide of the present invention. The host cell may also be a host cell capable of producing a beta-lactam compound, ie a host cell containing an organ that produces a beta-lactam compound. In some embodiments, the host cell is a bacterial, yeast or fibrous fungus. Fibrous fungi can be, for example, the genus Aspergillus or penicillium chrysogenum . The use of the Aspergillus genus for the expression of proteins is described, for example, in EP 272 277, EP 238 023 and EP 184 438. The use of genetically modified penicillium chrysogenum strains for the production of adifil -7-ADCA is described, for example, in EP 532 341.

The transformed host cell containing the construct can be cultured in a suitable nutrient medium under conditions that allow expression of the polypeptide and then the polypeptide can be recovered. Recovery can be accomplished, for example, by separating host cells from the medium by centrifugation or filtration. If the polypeptide is produced intracellularly, the cells are recovered from the fermentation medium by centrifugation or filtration and the cells are homogenized to release the polypeptide. The protein component is then precipitated from the medium or supernatant using a salt (eg ammonium sulfate) and subjected to various chromatography and / or electrophoretic procedures (eg ion exchange chromatography, gel filtration chromatography, isoelectric point ( isoelectric focusing), electrophoresis, affinity chromatography, and the like). Recovery techniques often depend on the nature of the particular polypeptide. The polypeptide can then be further used.

Alternatively or in addition, transformed host cells containing the constructs according to the invention are cultured in a suitable nutrient medium under conditions that allow expression of the beta-lactam compound in question.

In a fourth aspect, the present invention provides a method of culturing a transformed host cell containing a construct according to the invention under conditions favorable for the production of an N-acylated beta-lactam compound; Optionally, deacylating the resulting N-acylated beta-lactam compound to produce the corresponding N-deacylated compound; Provided is a method for preparing a beta-lactam compound, comprising recovering the N-acylated or N-deacylated beta-lactam compound. The skilled person will immediately recognize that the properties of the resulting beta-lactam compound will depend on the transformed host used. If the transformed host containing the construct according to the invention is a genetically modified penicillium chrysogenum strain modified to express an expandase , the N-deacylated beta-lactam compound is 7-ADCA. Alternatively, if the transformed host cell containing the construct according to the invention is an acremonium chrysogenum that naturally produces an expandase , the N-deacylated beta-lactam compound is 7-ACA.

The N-acyl side chain precursor to be used in the process carried out under conditions favorable for the production of N-acylated beta-lactam compounds is adipic acid or salts thereof. Alternatively, other side chain precursors can be used, for example those mentioned in International Application WO 95/04148, WO 95/04149, WO 98/48034 or WO 98/48035.

The arrangement of the vectors discussed above and the techniques required to produce transformed host cells are familiar to those skilled in the art, and all such aspects are within the scope of the present invention. In preparing expression cassettes, various polynucleotide elements can be engineered to be in the appropriate reading frame in the proper orientation. For this reason, adapters or linkers can be used to link the polynucleotide elements. In addition, various known procedures can be used for introducing convenient restriction enzyme sites, removing unnecessary nucleotides, removing restriction enzyme sites, and the like.

The polynucleotide sequences of the present invention can be used to identify and / or isolate corresponding sequences from other organisms, eg, other fungi. Sequences can be identified based on their degree of sequence identity to the sequences of the invention using methods known in the art, such as PCR, hybrids, etc., and sequences isolated based on their sequence identity to the sequences shown herein. Is included in the present invention. Methods suitable for such identification are known in the art and are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Plainview, New York), 1989. Described. For example, fragments of the nucleotide sequences disclosed herein can be used as probes. The probe can be specifically hybridized to the corresponding nucleotide sequence and messenger RNA and can be used, for example, to distinguish single base polymorphisms from different candidate sequences in SSCP analysis. In order to achieve specific hybridization under various conditions, the probe comprises different sequences between candidate sequences. The probe can be used to amplify the corresponding acyltransferase sequence by PCR from the selected organism; The technique can be used as a diagnostic assay to separate additional sequences from an organism or to determine the level of expression or presence of coding sequences in an organism.

The following examples are by way of example and not by way of limitation.

1 is a schematic of a 6343 bp vector (pET-penDE Pc) containing the T7 promoter, T7 terminator and kanamycin resistance gene.

2A-2E show an arrangement that provides the amino acid sequence of the acyltransferase enzymes disclosed herein.

Example 1

Cloning and Expression of Acyltransferase Genes

Acyl-Coenzyme A: Isopenicillin N Acyltransferase (acyltransferase) has been identified in several penicillin-producing organisms: penicillium chrysogenum ATCC9480 [Tobin et al., J. Bacteriol ., 172 (10) : 5908, 1990], Aspergillus nidulans ATCC38163 [Tobin et al., J. Bacteriol ., 172 (10): 5908, 1990], Penicillium nalgiovense ATCC10472 [Laich et al. , Appl. And Env. Microbiol ., 65 (3): 1236] and penicillium griseofulvum ATCC10120 [Laich et al., Appl. And Env. Microbiol ., 68 (3): 1211, 2002]. The genes can be cloned by culturing the organism, extracting total RNA, purifying mRNA and building a cDNA library using reverse transcriptase. Standard procedures for DNA technology are described, for example, in Sambrook, J., Fritsch, EF and Maniatis, T., Molecular Cloning: A Laboratory Manual , 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY , 1989). this. Standard conditions for the growth and manipulation of E. coli strains are described in Miller, J., Experiments in Molecular Genetics , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1972.

Genes can be isolated by designing and synthesizing PCR primers annealed at the 5 'and 3' ends of the acyltransferase gene, amplifying a specific gene from the cDNA library and cloning the expression vector. Alternatively, this example describes a method by which acyltransferase genes with known sequences can be synthesized starting from short oligonucleotides. Assembly of such synthetic genes is well described [Stemmer WP et al., Gene , 164 : 49, 1995; Jayaraman K. et al., Nucleic Acids Res ., 17 (11): 4403, 1989; Holowachuk EW et al., PCR Methods Appl . 4 (5): 299, 1995; Prodromou C et al., Protein Eng ., 5 (8): 827, 1992].

For example, penicillium Cri soge to exceed synthesizing acyltransferase (penDE) from ATCC9480, oligonucleotide duplication to encoding both strands of the penDE gene collectively nucleotides, and restriction site for commercial restriction endonuclease that sold under the Synthesize 5 'and 3' terminal sequences encoding NSA (US-QIAGEN Inc., Valencia, Calif.):

Oligonucleotides are mixed with each other at a uniform concentration of 250 μM. The mixture was 10 mM Tris -HCl (pH 9.0) /2.2 mM MgCl 2/50 mM KCl / 0.1% Triton X-100 [1 x cloned Pfu buffer (stripe Teijin (Stratagene), La Jolla, California, USA)] / Taq DNA polymerase (Promega, Madison, WI) /0.02 U of Pfu DNA polymerase (Strate) of deoxynucleotide (stratizine, La Jolla, Calif.) / 1 U each 0.2 mM Dilute 100-fold in 20 μl PCR mixture containing Gin, La Jolla, CA. The gene was thermocycler (MJ Research PTC-150 minicycler (MJ Research, Ph.D.), which was programmed for 55 cycles of 30 seconds at 94 ° C, 30 seconds at 52 ° C, and 30 seconds at 72 ° C. Corporate, Leno, Nevada, USA]. The resulting mixture gene assembly 10 mM Tris -HCl (pH 9.0) /2.2 mM MgCl 2/50 mM KCl / 0.1% Triton X-100 [1 x cloned Pfu buffer (stripe Teijin, La Jolla, Calif.); Taq DNA polymerase (Promega, Madison, WI) / 5 U each of deoxynucleotides (Stratein, La Jolla, Calif.) / 0.2 U of Pfu DNA polymerase (Stratizin, Dilute 40-fold in 100 μl of PCR mixture containing two external primers at La Jolla, California, USA / 1 μM concentration. The two outer primers may be identical to two oligonucleotides representing the 5 'end of the positive and negative strands. In this example, the outer primers annealed at the 5'-end of the positive strand are Nde I recognition sites ( The second half of ATG overlaps the initiation codon of the acyltransferase gene) and the outer primer annealed at the 5'-end of the negative strand adds the Not I recognition site. The gene is a thermocycler fitted with a 23 cycle program of 30 seconds at 94 ° C, 30 seconds at 50 ° C, and 60 seconds at 72 ° C [MJ Research PTC-150 Minicycler (MJ Research, Incorporated, Nevada, USA). Leno). Final genes were purified using QIAquick PCR Purification Kit (USA-Kiagen Incorporated, Valencia, Calif.), And Nde I and Not I (New England Biolabs, Massachusetts, USA). Cut into Beverly, and the resulting 1.0 kb fragment is purified using Kiaquick gel extraction kit (USA-Kiagen Inc., Valencia, Calif.). Synthetic penDE gene from Penicillium chrysogenum ATCC9480 can be cloned into an appropriate expression vector. All other acyltransferase genes described herein can be constructed using similar procedures by introducing appropriate changes in the oligonucleotide sequence (reflecting changes in sequence for the penDE gene from Penicillium chrysogenum ATCC9480).

Plasmid pET24B has been described in Studier (Moffatt, BA, et al., J. Mol. Biol ., 189 : 113, 1986 and by Novagen (Novagen Incorporated, Madison, WI) . One of the vectors of the pET series further developed to facilitate cloning, detection and purification of recombinant proteins in E. coli . These vectors typically have the colysine El replicon of pBR322 and confer resistance to ampicillin or kanamycin. There are two general classes of vectors in the pET series: transcription and translation vectors. Translation vectors containing highly effective ribosomal binding sites from phage T7 are designed to express genes that do not have their own ribosomal binding sites. Alternatively, a transcription vector for expressing a target gene having its own prokaryotic ribosomal binding site is designed. How expression of active acyltransferase enzymes can be performed by the use of plasmid pET24B (translation vector) to clone an acyltransferase gene from penicillium chrysogenum ATCC9480. Multiple Cloning Site (MCS) of pET24B includes both Nde I and Not I recognition sequences to enable directional cloning of the synthesized acyltransferase gene. pET24B was cleaved with Nde I and Not I (New England Biolabs, Beverly, Mass.) and purified using Kiaquick Gel Extraction Kit (USA-Kiagen Inc., Valencia, Calif.) to obtain appropriate expression vectors. Create The complementary viscous ends of the two fragments (pET24B vector and acyltransferase insert) are conjugated using a T4 DNA ligase kit (New England Biolabs, Beverly, Mass.). Once the acyltransferase fragments were conjugated into the pET24B plasmid to produce a new plasmid called pET-penDE Pc (FIG. 1), the recombinant plasmid was extracted . Replication and expression of genes can be confirmed by transforming E. coli BL21 cells (Novagen Inc., Madison, Wisconsin) and then selecting cells based on the expression of the antibiotic resistance gene contained on the plasmid. For pET24B, kanamycin is used as a selection marker for cells containing an intact and actable plasmid, which may or may not have an acyltransferase gene. A few clones are picked out and grown in liquid culture and DNA is prepared using the QIAprep Spin Miniprep Kit (USA-Kiagen Inc., Valencia, CA, USA). DNA was sequenced on an ABI 3700 DNA analyzer (Applied Biosystems, Inc., Foster City, Calif.) Using pET sequencing primer (Novagen Inc., Madison, Wisconsin), and the correct acyltransferase sequence. One clone with is selected. The properties of acyltransferase activity are described in Example 2.

Example 2

Expression of Acyl Transferase

Plasmid pET-penDE Pc was chemically antibody-reactive. E. coli BL21 (DE3) cells (Novagen Inc., Madison, WI) were transformed, 1% glucose (Sigma, St. Louis, MO) and chloramphenicol (30 μg / ml) (Sigma, The plate is plated on LB agar medium (EM Science, Gibbstown, NJ) containing St. Louis, Missouri. Individual colonies formed on the plates are picked out and used to inoculate 2 × YT medium (EM Science, Gibbstown, NJ) containing 0.5% glucose and chloramphenicol. Incorporation of glucose in the medium is important for reducing early gene expression, which can be harmful to cell growth. Cultures are grown overnight in a shake incubator at 37 ° C. A small amount of production culture is diluted to a cell density of about 0.05 A ₆₀₀ in fresh LB medium containing 0.5% glucose and chloramphenicol and the culture is grown to an initial log phase (approximate cell density of 0.5 A ₆₀₀ or higher). 0.2 mM isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma, St. Louis, MO) was added to induce the expression of the acyltransferase gene, and the cultures were shaken at 28 ° C. for 16 hours. Incubate further. Cells are harvested by centrifugation at 4000 rpm at 4 ° C. and washed three or more times with 5 mM morpholine (Sigma, St. Louis, MO) buffer (pH 7.5) to remove any undesirable salt residues. Washed cells were treated with 100 μl of lysis buffer (2 mg / ml polymyxin B sulfate (PMBS) (Amersham Biosciences, Newark, NJ)), 10 U / μl of lysozyme (Epi Center Epicentre, Madison, Wisconsin, 1 U / μl of RNase (Epicenter, Madison, Wisconsin) and 10 U / μl DNase (Epicenter, Madison, Wisconsin, USA) and resuspend at room temperature.

Example 3

Properties of Acyltransferase Activity

This assay is used to assess acyltransferase specific activity of converting 6-APA and Ad-CoA substrates into Ad-6-APA products. 1.5 mM 6-APA, 50 mM DTT and 0.6 mM Ad-CoA were dissolved in 5 mM morpholine buffer (pH 7.5) respectively to separate separate stock solutions of 1.5 mM 6-APA, 50 mM DTT and 0.6 mM Ad-CoA. Manufacture newly. 6-APA and DTT are commercially available from Sigma, St. Louis, Missouri. Adipoyl-coenzyme A is obtained as follows: Adipoyl chloride (0.14 ml; 6.95 mmol) is coenzyme in acetone (60 ml) + water (0.6 ml) + 0.2 M KHCO ₃ (for pH rise) under nitrogen atmosphere. A sodium salt (400 mg, 0.48 mmol) is added to a well stirred, ice-cold suspension. The pH of the reaction is brought to about 7.7 and stirred for about 60 minutes. Acetone is then partially removed under reduced pressure, and the product is dissolved in cold water and freeze-dried. Finally, the product is subjected to ultrafiltration. The yield is 0.9 g (analysis 70%). The water used is treated with a nitrogen gas stream to exclude the oxidation of coenzyme A.

Mix 8 ml of 1.5 mM 6-APA, 4 ml of 0.6 mM Ad-CoA and 4 ml of 50 mM DTT to 0.75 mM 6-APA, 0.15 mM Ad-CoA, 12.5 mM DTT, 5 mM morpholine buffer (pH Create the master solution of 7.5). After lysis of the cells of Example 2 for 30 minutes at room temperature, 300 μl of 5 mM morpholine buffer (pH 7.5) is added to the solution. To initiate the acyltransferase reaction, 155 μl of 5 mM morpholine buffer is transferred to the wells of tubes or 96-well plates. 20 μl of acyltransferase enzyme diluted 1: 4 from 30 μl fresh master solution (0.75 mM 6-APA, 0.15 mM Ad-CoA, 12.5 mM DTT, 5 mM morpholine buffer, pH 7.5) and total lysate Add. Pipette the enzyme and master solution 2-3 times to mix lightly. The reaction is allowed to proceed for 3 minutes at room temperature. Transfer 60 μl of the reaction mixture to a fresh microplate or vessel containing 60 μl of ice cooled methanol and mix well to stop the reaction. Optimally, the sample is filtered through, for example, Whatman custom filter plates (1 μm pore size) (Watman Inc., Clipton, NJ) and the filtrate is Nunc V -Collected on bottom polystyrene microplates (Genk, Rochester, NY). The plate is sealed with normal aluminum foil.

Ad-6-APA products can be detected using mass spectroscopy. Electrospray ionization mass spectroscopy (ESI / MS) analysis can be performed on a Quattro Ultima triple-four mass spectrometer (Micromass, Manchester, UK) with an electrospray ion source. Typically, the sample is dissolved in methanol / water (1: 1) as described in Example 2. Adipoyl-6-APA will produce strong deprotonation molecules ([M-H]-) in an anionic manner. The capillary voltage is 3.0 kV, the source temperature is 120 ° C and the desolvation temperature is 250 ° C. Adipoyl-6-APA is monitored by mass change from 343.0 to 265.0 under cone energy of 15 V and collision energy of 15 eV. Ad-6-APA can be quantified by comparing the sample peak area to a standard curve. The standard curve is estimated by injecting Ad-6-APA samples of known concentration and measuring the peak area.

For flow injection analysis (FIA), 0.5 ml / min for a 1100 Series (Agilent, San Jose, CA) LC system equipped with an HTP pal (CTC analysis, Zvingen, Switzerland) autosampler It is used to transfer the mobile phase at a flow rate of. Inject 5 μl of sample. The mobile phase is isocratic elution in 70% methanol and 30% water.

SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48 Acyltransferase polypeptides corresponding to sequences 50, 50, and 52 all have greater acyltransferase activity (generated Ad-6-APA) than wild-type acyltransferase (SEQ ID NO: 54) from penicillium chrysogenum in this assay. Corresponding to the amount of the product).

The results are shown in Table 1.

All publications and patent applications mentioned in the specification are indicative of those skilled in the art to which this invention pertains. All publications and patent applications are incorporated herein by reference in the same manner as when each individual publication or patent application was specifically and individually incorporated by reference. Subtitles in the specification record are included only for ease of review of the record and are not intended to limit the content of the record in any way.

While the invention has been described in some detail by way of illustration and example for clarity of understanding, it is apparent that certain changes and modifications may be made within the scope of the appended claims.

                                SEQUENCE LISTING <110> DSM IP Assets B.V. <120> Altered Acyltransferase Polypeptides and Related Polynucleotides    <130> 24315WO <140> <141> <160> 110 FastSEQ for Windows Version 4.0 <210> 1 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H1 <221> CDS (222) (1) ... (1074) <400> 1 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gat gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 2 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H1 <400> 2 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 3 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H2 <221> CDS (222) (1) ... (1074) <400> 3 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc cag gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gta ttt ggt ttg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Val Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt cag cac ggc aaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255   aaa gag ctc gat ccc tta ccg gac tca tgg aat cgc cac cag cgt atg 816 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc gac ggg ttc gac ggc acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gcc gac gaa gac aat tat ccc ttt agc atc tgc cgc gct tac 912 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 4 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H2 <400> 4 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Val Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 5 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H3 <221> CDS (222) (1) ... (1074) <400> 5 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc cag gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttt ggt ttg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt cag cac ggc aaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255   aaa gag ctc gat ccc tta ccg gac tca tgg aat cgc cac cag cgt atg 816 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc gac ggg ttc gac ggc acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gcc gac gaa gac aat tat ccc ttt agc atc tgc cgc gct tac 912 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 6 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H3 <400> 6 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 7 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H4 <221> CDS (222) (1) ... (1074) <400> 7 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac caa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Gln  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc acc gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc gac ggg ttc gac ggc acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 8 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H4 <400> 8 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Gln  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 9 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H5 <221> CDS (222) (1) ... (1074) <400> 9 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ttg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ctc ata acc gag gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Leu Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 10 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H5 <400> 10 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Leu Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 11 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H6 <221> CDS (222) (1) ... (1074) <400> 11 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gag cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttc gca tac ggc ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggt tgc acc acc gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tcc gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttt ggt ttg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 12 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H6 <400> 12 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 13 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H7 <221> CDS (222) (1) ... (1074) <400> 13 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gcg aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa cta cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggc ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac ccc 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 14 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H7 <400> 14 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 15 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H14 <221> CDS (222) (1) ... (1074) <400> 15 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gag cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttc gca tac ggc ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc caa ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gat gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 16 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H14 <400> 16 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 17 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H25 <221> CDS (222) (1) ... (1074) <400> 17 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttc gca tac ggc ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggt tgc acc acc gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cgg 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Arg         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac ccc 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 18 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase 25 <400> 18 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Arg         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 19 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H26 <221> CDS (222) (1) ... (1074) <400> 19 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aac 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc gac ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac ccc 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 20 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H26 <400> 20 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 21 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H27 <221> CDS (222) (1) ... (1074) <400> 21 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gac ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tcc gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 22 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H27 <400> 22 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 23 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H28 <221> CDS (222) (1) ... (1074) <400> 23 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gac ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ctc ata acc gag gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Leu Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc cag gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 24 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H28 <400> 24 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Leu Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 25 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H29 <221> CDS (222) (1) ... (1074) <400> 25 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 26 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H29 <400> 26 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 27 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H30 <221> CDS (222) (1) ... (1074) <400> 27 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat ttc aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Phe Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt agc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 28 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H30 <400> 28 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Phe Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 29 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H31 <221> CDS (222) (1) ... (1074) <400> 29 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gag cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttc gca tac ggc ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tcc gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc cag gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aac ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gat gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 30 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H31 <400> 30 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 31 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H32 <221> CDS (222) (1) ... (1074) <400> 31 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttc gca tac ggc ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc caa ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggg aag gtc gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gat gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 32 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H32 <400> 32 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 33 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H33 <221> CDS (222) (1) ... (1074) <400> 33 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 34 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H33 <400> 34 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 35 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H34 <221> CDS (222) (1) ... (1074) <400> 35 atg ctt cac atc ctc tgt caa ggc act ccc ttc gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggc ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tat gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 36 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H34 <400> 36 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 37 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H35 <221> CDS (222) (1) ... (1074) <400> 37 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc tcc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Ser Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aat ttc aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Phe Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat gcg cac gag gcc tat ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Tyr Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ctg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tca tgg agt cgc cac ggg cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Ser Arg His Gly Arg Met             260 265 270   gag cac ctc ctc tcc ggg ttc gac ggc acc aaa gag gca ttt gcc aag 864 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Glu Ala Phe Ala Lys         275 280 285   ttg tgg gag gac gaa gac aat tat ccc ttt agc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 38 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H35 <400> 38 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Ser Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Phe Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Tyr Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Ser Arg His Gly Arg Met             260 265 270 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Glu Ala Phe Ala Lys         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 39 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H36 <221> CDS (222) (1) ... (1074) <400> 39 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gag aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   atg ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cat ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat att gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa aac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asn Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 40 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H36 <400> 40 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asn Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 41 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H37 <221> CDS (222) (1) ... (1074) <400> 41 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt act gcg acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Thr Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggc gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat ctt gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc gag gcc tat gaa aag atc gtg tcg caa ggc gga atg gcc 624 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205   gcc acc gct ttt atc atg gtg ggc aat gcg cac gag gca ttc ggt ctg 672 Ala Thr Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc tcc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg acc ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 42 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H37 <400> 42 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Thr Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205 Ala Thr Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 43 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H38 <221> CDS (222) (1) ... (1074) <400> 43 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att tgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gtc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc tgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Cys Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gaa gct gga atc atc ggc aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctc ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gaa ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag acg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Thr Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 44 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H38 <400> 44 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Cys Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Thr Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 45 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H40 <221> CDS (222) (1) ... (1074) <400> 45 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   atg ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cat ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat att gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 46 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H40 <400> 46 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 47 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H41 <221> CDS (222) (1) ... (1047) <400> 47 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggc gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat ctt gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc gag gcc tat gaa aag atc gtg tcg caa ggc gga atg gcc 624 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat gcg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc tcc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg acc ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag aggtctgcgc 1057 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu             340 345   tcaacgccag gctttga 1074 <210> 48 <211> 349 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H41 <400> 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu             340 345 <210> 49 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H42 <221> CDS (222) (1) ... (1074) <400> 49 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att tgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gtc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   atg ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggg gtc gcc gtc aac tac aac gcc ctt cac ctt cat ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat att gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc caa gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg ttg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc ggc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aat gcc aag ctt tga 1074 Leu Asn Ala Lys Leu *         355   <210> 50 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H42 <400> 50 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Ile Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Leu Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Gly Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Lys Leu         355 <210> 51 <211> 1074 <212> DNA <213> Artificial Sequence <220> <223> H43 <221> CDS (222) (1) ... (1074) <400> 51 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aga 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg      50 55 60   tac tac gag gag att tgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   agg gca gcc cgt gat ggc tgc acc act gtc tat tgt caa ctt cca aat 336 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggc gtc gcc gtc aac tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga ctt cct tcg cat ctt gcc ctc cgc atg gcg ctc gaa agc act 576 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct cct tcc gag gcc tat gaa aag atc gtg tcg caa ggc gga atg gcc 624 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat gcg cac gag gca ttc ggt ctg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220   gaa ttt tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt ccc cac ggc gaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255   aaa gag atc gat ccc tta ccg gac tcg tgg aat cgc cac cag cgt atg 816 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag cac ctc ctc tcc ggg ttc gac ggt acc aaa cag gca ttt gcc cag 864 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gag gac gaa gac aat tat ccc ttt ggc atc tgc cgc gct tac 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300   gag gta ggc aag agc aga ggc gcg acc ctg ttc aat atc atc tac gac 960 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgt ttt gac gag gag gat gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 52 <211> 357 <212> PRT <213> Artificial Sequence <220> <223> Acyltransferase H43 <400> 52 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Arg Ala Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Pro His Gly Glu Asn Glu                 245 250 255 Lys Glu Ile Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Phe Gly Ile Cys Arg Ala Tyr     290 295 300 Glu Val Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 53 <211> 1074 <212> DNA <213> Penicillium chrysogenum <220> <221> CDS (222) (1) ... (1074) <400> 53 atg ctt cac atc ctc tgt caa ggc act ccc ttt gaa atc ggc tac gaa 48 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15   cat ggc tct gct gcc aaa gcc gtg ata gcc aga agc att gac ttc gcc 96 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala              20 25 30   gtc gat ctc atc cga ggg aaa acg aag aag acg gac gaa gag ctt aaa 144 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys          35 40 45   cag gta ctc tcg caa ctg ggg cgc gtg atc gag gaa aga tgg ccc aaa 192 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys      50 55 60   tac tac gag gag att cgc ggt att gca aag ggc gct gaa cgc gat gtc 240 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val  65 70 75 80   tcc gag att gtc atg ctt aat acc cgc acg gaa ttt gca tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   aag gca gcc cgt gat ggc tgc acc act gcc tat tgt caa ctt cca aat 336 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110   gga gcc ctc cag ggc caa aac tgg gat ttc ttt tct gcc acc aaa gag 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125   aac ctg atc cgg tta acg atc cgt cag gcc gga ctt ccc acc atc aaa 432 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140   ttc ata acc gag gct gga atc atc ggg aag gtt gga ttt aac agt gcg 480 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggc gtc gcc gtc aat tac aac gcc ctt cac ctt cag ggt ctt cga ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175   acc gga gtt cct tcg cat att gcc ctc cgc ata gcg ctc gaa agc act 576 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190   tct cct tcc cag gcc tat gac cgg atc gtg gag caa ggc gga atg gcc 624 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205   gcc agc gct ttt atc atg gtg ggc aat ggg cac gag gca ttt ggt ttg 672 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220   gaa ttc tcc ccc acc agc atc cga aag cag gtg ctc gac gcg aat ggt 720 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240   agg atg gtg cac acc aac cac tgc ttg ctt cag cac ggc aaa aat gag 768 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255   aaa gag ctc gat ccc tta ccg gac tca tgg aat cgc cac cag cgt atg 816 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270   gag ttc ctc ctc gac ggg ttc gac ggc acc aaa cag gca ttt gcc cag 864 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285   ctc tgg gcc gac gaa gac aat tat ccc ttt agc atc tgc cgc gct tac 912 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300   gag gag ggc aag agc aga ggc gcg act ctg ttc aat atc atc tac gac 960 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320   cat gcc cgt aga gag gca acg gtg cgg ctt ggc cgg ccg acc aac cct 1008 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335   gat gag atg ttt gtc atg cgg ttt gac gag gag gac gag agg tct gcg 1056 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350   ctc aac gcc agg ctt tga 1074 Leu Asn Ala Arg Leu *         355   <210> 54 <211> 357 <212> PRT <213> Penicillium chrysogenum acyltransferase <400> 54 Met Leu His Ile Leu Cys Gln Gly Thr Pro Phe Glu Ile Gly Tyr Glu  1 5 10 15 His Gly Ser Ala Ala Lys Ala Val Ile Ala Arg Ser Ile Asp Phe Ala             20 25 30 Val Asp Leu Ile Arg Gly Lys Thr Lys Lys Thr Asp Glu Glu Leu Lys         35 40 45 Gln Val Leu Ser Gln Leu Gly Arg Val Ile Glu Glu Arg Trp Pro Lys     50 55 60 Tyr Tyr Glu Glu Ile Arg Gly Ile Ala Lys Gly Ala Glu Arg Asp Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Lys Ala Ala Arg Asp Gly Cys Thr Thr Ala Tyr Cys Gln Leu Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Ser Ala Thr Lys Glu         115 120 125 Asn Leu Ile Arg Leu Thr Ile Arg Gln Ala Gly Leu Pro Thr Ile Lys     130 135 140 Phe Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu Gln Gly Leu Arg Pro                 165 170 175 Thr Gly Val Pro Ser His Ile Ala Leu Arg Ile Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Gln Ala Tyr Asp Arg Ile Val Glu Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Gly His Glu Ala Phe Gly Leu     210 215 220 Glu Phe Ser Pro Thr Ser Ile Arg Lys Gln Val Leu Asp Ala Asn Gly 225 230 235 240 Arg Met Val His Thr Asn His Cys Leu Leu Gln His Gly Lys Asn Glu                 245 250 255 Lys Glu Leu Asp Pro Leu Pro Asp Ser Trp Asn Arg His Gln Arg Met             260 265 270 Glu Phe Leu Leu Asp Gly Phe Asp Gly Thr Lys Gln Ala Phe Ala Gln         275 280 285 Leu Trp Ala Asp Glu Asp Asn Tyr Pro Phe Ser Ile Cys Arg Ala Tyr     290 295 300 Glu Glu Gly Lys Ser Arg Gly Ala Thr Leu Phe Asn Ile Ile Tyr Asp 305 310 315 320 His Ala Arg Arg Glu Ala Thr Val Arg Leu Gly Arg Pro Thr Asn Pro                 325 330 335 Asp Glu Met Phe Val Met Arg Phe Asp Glu Glu Asp Glu Arg Ser Ala             340 345 350 Leu Asn Ala Arg Leu         355 <210> 55 <211> 1074 <212> DNA <213> Aspergillus nidulans <220> <221> CDS (222) (1) ... (1074) <400> 55 atg ctt cac gta act tgc caa ggt acc ccc tcc gaa atc ggc tat cac 48 Met Leu His Val Thr Cys Gln Gly Thr Pro Ser Glu Ile Gly Tyr His  1 5 10 15   cat ggc tct gct gcc aaa ggc gag att gcg aaa gcc att gac ttc gca 96 His Gly Ser Ala Ala Lys Gly Glu Ile Ala Lys Ala Ile Asp Phe Ala              20 25 30   act ggc ctc att cat ggc aaa aca aaa aag aca cag gcg gag ctt gaa 144 Thr Gly Leu Ile His Gly Lys Thr Lys Lys Thr Gln Ala Glu Leu Glu          35 40 45   cag ctc ctc agg gag ttg gag cag gtg atg aaa cag cgc tgg ccg aga 192 Gln Leu Leu Arg Glu Leu Glu Gln Val Met Lys Gln Arg Trp Pro Arg      50 55 60   tac tat gag gaa atc tgc gga atc gca aag ggt gcg gaa cgc gaa gta 240 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Glu Val  65 70 75 80   tcg gag att gtc atg ctc aac act cgt acg gaa ttc gcg tac ggg ctc 288 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                  85 90 95   gta gaa gcc cgg gac ggg tgc acc act gtt tac tgc aaa acc ccc aat 336 Val Glu Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Lys Thr Pro Asn             100 105 110   gga gcg cta cag ggc cag aac tgg gac ttc ttc acc gca acc aaa gaa 384 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Thr Ala Thr Lys Glu         115 120 125   aac ttg atc cag tta aca att tgt cag ccg ggt cta ccc act atc aaa 432 Asn Leu Ile Gln Leu Thr Ile Cys Gln Pro Gly Leu Pro Thr Ile Lys     130 135 140   atg att aca gaa gct ggt atc att ggc aaa gtg ggt ttc aac agt gct 480 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160   ggt gtc gct gtc aat tac aat gca cta cac cta cat ggc ctc cgt ccc 528 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175   act ggc ctc ccc tcg cat ctc gcg ctg cgc atg gcc ctc gaa agt aca 576 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190   tct ccg tct gag gcg tat gaa aaa atc gtc tcg caa ggg ggc atg gcg 624 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205   gct agc gcg ttc atc atg gtg ggc aac gca cac gag gcc tac ggg cta 672 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Tyr Gly Leu     210 215 220   gag ttc tcg ccc atc agc ttg tgc aag caa gtt gct gac acc aat ggg 720 Glu Phe Ser Pro Ile Ser Leu Cys Lys Gln Val Ala Asp Thr Asn Gly 225 230 235 240   cgg ata gtg cat acg aac cac tgc ctc ctc aac cat ggg cca tcg gcg 768 Arg Ile Val His Thr Asn His Cys Leu Leu Asn His Gly Pro Ser Ala                 245 250 255   caa gag ctt aat ccc ctg ccg gac tcg tgg agc cgc cac ggg cgg atg 816 Gln Glu Leu Asn Pro Leu Pro Asp Ser Trp Ser Arg His Gly Arg Met             260 265 270   gaa cat ctc ctc tct ggt ttt gac ggc acg aag gag gca ttt gcg aag 864 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Glu Ala Phe Ala Lys         275 280 285   ttg tgg gag gac gaa gac aac tac cct ctc tcg atc tgc cgg gca tat 912 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Leu Ser Ile Cys Arg Ala Tyr     290 295 300   aag gaa ggg aaa agt aga ggc tcc act ctt ttc aac atc gtc ttc gat 960 Lys Glu Gly Lys Ser Arg Gly Ser Thr Leu Phe Asn Ile Val Phe Asp 305 310 315 320   cat gtg ggc cgg aag gca aca gtg cgg ctg ggc cgg ccc aat aac cct 1008 His Val Gly Arg Lys Ala Thr Val Arg Leu Gly Arg Pro Asn Asn Pro                 325 330 335   gat gag acc ttt gtc atg acc ttt agc aat ctg gat acc aag tcc gcg 1056 Asp Glu Thr Phe Val Met Thr Phe Ser Asn Leu Asp Thr Lys Ser Ala             340 345 350   atc caa gcc aac att tga 1074 Ile Gln Ala Asn Ile *         355   <210> 56 <211> 357 <212> PRT <213> Aspergillus nidulans acyltransferase <400> 56 Met Leu His Val Thr Cys Gln Gly Thr Pro Ser Glu Ile Gly Tyr His  1 5 10 15 His Gly Ser Ala Ala Lys Gly Glu Ile Ala Lys Ala Ile Asp Phe Ala             20 25 30 Thr Gly Leu Ile His Gly Lys Thr Lys Lys Thr Gln Ala Glu Leu Glu         35 40 45 Gln Leu Leu Arg Glu Leu Glu Gln Val Met Lys Gln Arg Trp Pro Arg     50 55 60 Tyr Tyr Glu Glu Ile Cys Gly Ile Ala Lys Gly Ala Glu Arg Glu Val 65 70 75 80 Ser Glu Ile Val Met Leu Asn Thr Arg Thr Glu Phe Ala Tyr Gly Leu                 85 90 95 Val Glu Ala Arg Asp Gly Cys Thr Thr Val Tyr Cys Lys Thr Pro Asn             100 105 110 Gly Ala Leu Gln Gly Gln Asn Trp Asp Phe Phe Thr Ala Thr Lys Glu         115 120 125 Asn Leu Ile Gln Leu Thr Ile Cys Gln Pro Gly Leu Pro Thr Ile Lys     130 135 140 Met Ile Thr Glu Ala Gly Ile Ile Gly Lys Val Gly Phe Asn Ser Ala 145 150 155 160 Gly Val Ala Val Asn Tyr Asn Ala Leu His Leu His Gly Leu Arg Pro                 165 170 175 Thr Gly Leu Pro Ser His Leu Ala Leu Arg Met Ala Leu Glu Ser Thr             180 185 190 Ser Pro Ser Glu Ala Tyr Glu Lys Ile Val Ser Gln Gly Gly Met Ala         195 200 205 Ala Ser Ala Phe Ile Met Val Gly Asn Ala His Glu Ala Tyr Gly Leu     210 215 220 Glu Phe Ser Pro Ile Ser Leu Cys Lys Gln Val Ala Asp Thr Asn Gly 225 230 235 240 Arg Ile Val His Thr Asn His Cys Leu Leu Asn His Gly Pro Ser Ala                 245 250 255 Gln Glu Leu Asn Pro Leu Pro Asp Ser Trp Ser Arg His Gly Arg Met             260 265 270 Glu His Leu Leu Ser Gly Phe Asp Gly Thr Lys Glu Ala Phe Ala Lys         275 280 285 Leu Trp Glu Asp Glu Asp Asn Tyr Pro Leu Ser Ile Cys Arg Ala Tyr     290 295 300 Lys Glu Gly Lys Ser Arg Gly Ser Thr Leu Phe Asn Ile Val Phe Asp 305 310 315 320 His Val Gly Arg Lys Ala Thr Val Arg Leu Gly Arg Pro Asn Asn Pro                 325 330 335 Asp Glu Thr Phe Val Met Thr Phe Ser Asn Leu Asp Thr Lys Ser Ala             340 345 350 Ile Gln Ala Asn Ile         355 <210> 57 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 57 tctagacata tgcttcacat cctctgtcaa ggcactccc 39 <210> 58 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 58 tttgaaatcg gctacgaaca tggctctgct gccaaagcc 39 <210> 59 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 59 gtgatagcca gaagcattga cttcgccgtc gatctcatcc ga 42 <210> 60 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 60 gggaaaacga agaagacgga cgaagagctt aaacaggta 39 <210> 61 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 61 ctctcgcaac tggggcgcgt gatcgaggaa agatggccc 39 <210> 62 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 62 aaatactacg aggagattcg cggtattgca aagggcgctg aa 42 <210> 63 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 63 cgcgatgtct ccgagattgt catgcttaat acccgcacg 39 <210> 64 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 64 gaatttgcat acgggctcaa ggcagcccgt gatggctgc 39 <210> 65 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 65 accactgcct attgtcaact tccaaatgga gccctccagg gc 42 <210> 66 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 66 caaaactggg atttcttttc tgccaccaaa gagaacctg 39 <210> 67 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 67 atccggttaa cgatccgtca ggccggactt cccaccatc 39 <210> 68 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 68 aaattcataa ccgaggctgg aatcatcggg aaggttggat tt 42 <210> 69 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 69 aacagtgcgg gcgtcgccgt caattacaac gcccttcac 39 <210> 70 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 70 cttcagggtc ttcgacccac cggagttcct tcgcatatt 39 <210> 71 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 71 gccctccgca tagcgctcga aagcacttct ccttcccagg cc 42 <210> 72 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 72 tatgaccgga tcgtggagca aggcggaatg gccgccagc 39 <210> 73 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 73 gcttttatca tggtgggcaa tgggcacgag gcatttggt 39 <210> 74 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 74 ttggaattct cccccaccag catccgaaag caggtgctcg ac 42 <210> 75 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 75 gcgaatggta ggatggtgca caccaaccac tgcttgctt 39 <210> 76 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 76 cagcacggca aaaatgagaa agagctcgat cccttaccg 39 <210> 77 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 77 gactcatgga atcgccacca gcgtatggag ttcctcctcg ac 42 <210> 78 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 78 gggttcgacg gcaccaaaca ggcatttgcc cagctctgg 39 <210> 79 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 79 gccgacgaag acaattatcc ctttagcatc tgccgcgct 39 <210> 80 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 80 tacgaggagg gcaagagcag aggcgcgact ctgttcaata tc 42 <210> 81 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 81 atctacgacc atgcccgtag agaggcaacg gtgcggctt 39 <210> 82 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 82 ggccggccga ccaaccctga tgagatgttt gtcatgcgg 39 <210> 83 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 83 tttgacgagg aggacgagag gtctgcgctc aacgccaggc tt 42 <210> 84 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 84 atgttcgtag ccgatttcaa agggagtgcc ttgacagagg at 42 <210> 85 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 85 gtcaatgctt ctggctatca cggctttggc agcagagcc 39 <210> 86 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 86 cgtcttcttc gttttccctc ggatgagatc gacggcgaa 39 <210> 87 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 87 cacgcgcccc agttgcgaga gtacctgttt aagctcttcg tc 42 <210> 88 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 88 gcgaatctcc tcgtagtatt tgggccatct ttcctcgat 39 <210> 89 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 89 aatctcggag acatcgcgtt cagcgccctt tgcaatacc 39 <210> 90 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 90 cttgagcccg tatgcaaatt ccgtgcgggt attaagcatg ac 42 <210> 91 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 91 aagttgacaa taggcagtgg tgcagccatc acgggctgc 39 <210> 92 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 92 aaagaaatcc cagttttggc cctggagggc tccatttgg 39 <210> 93 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 93 ctgacggatc gttaaccgga tcaggttctc tttggtggca ga 42 <210> 94 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 94 tccagcctcg gttatgaatt tgatggtggg aagtccggc 39 <210> 95 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 95 ggcgacgccc gcactgttaa atccaacctt cccgatgat 39 <210> 96 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 96 ggtgggtcga agaccctgaa ggtgaagggc gttgtaattg ac 42 <210> 97 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 97 ttcgagcgct atgcggaggg caatatgcga aggaactcc 39 <210> 98 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 98 ctccacgatc cggtcatagg cctgggaagg agaagtgct 39 <210> 99 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 99 attgcccacc atgataaaag cgctggcggc cattccgcct tg 42 <210> 100 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 100 gctggtgggg gagaattcca aaccaaatgc ctcgtgccc 39 <210> 101 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 101 caccatccta ccattcgcgt cgagcacctg ctttcggat 39 <210> 102 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 102 tttctcattt ttgccgtgct gaagcaagca gtggttggtg tg 42 <210> 103 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 103 ctggtggcga ttccatgagt ccggtaaggg atcgagctc 39 <210> 104 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 104 tttggtgccg tcgaacccgt cgaggaggaa ctccatacg 39 <210> 105 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 105 gggataattg tcttcgtcgg cccagagctg ggcaaatgcc tg 42 <210> 106 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 106 tctgctcttg ccctcctcgt aagcgcggca gatgctaaa 39 <210> 107 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 107 acgggcatgg tcgtagatga tattgaacag agtcgcgcc 39 <210> 108 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 108 atcagggttg gtcggccggc caagccgcac cgttgcctct ct 42 <210> 109 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 109 cctctcgtcc tcctcgtcaa accgcatgac aaacatctc 39 <210> 110 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> Synthetic oligonucleotide <400> 110 cgtcatgaag agccttcaaa gcctggcgtt gagcgcaga 39  

Claims (21)

An isolated polypeptide having acyltransferase activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38; (b) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42; (c) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52; (d) has an amino acid sequence of 96% or more identical to SEQ ID NO: 54, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, and position 277 A polypeptide having one or more residues selected from the group consisting of Gly, Glu at position 291 and Gly at position 299; And (e) A polypeptide encoded by a polynucleotide that hybridizes under stringent conditions on a substantially full length nucleotide sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 42 and SEQ ID NO: 52. The method of claim 1, An isolated polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38. The method of claim 1, An isolated polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42. The method of claim 1, An isolated polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52. The method of claim 1, SEQ ID NO: 54 having an amino acid sequence of 96% or more, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, Gly at position 277, and An isolated polypeptide having one or more residues selected from the group consisting of Glu at position 291 and Gly at position 299. The method of claim 5, An isolated polypeptide comprising Arg at position 97 of the amino acid sequence. The method of claim 5, An isolated polypeptide wherein the amino acid sequence comprises Val at position 221. The method of claim 5, The amino acid sequence is Arg at position 97, Val at position 221, Val at position 251, Pro at position 251, Glu at position 254, Ile at position 259, Gly at position 277, Glu at position 291 and G99 at position 299. An isolated polypeptide comprising two or more residues selected from the group consisting of Gly. The method of claim 8, The amino acid sequence is Arg at position 97, Val at position 221, Val at position 251, Pro at position 251, Glu at position 254, Ile at position 259, Gly at position 277, Glu at position 291 and G99 at position 299. An isolated polypeptide comprising four or more residues selected from the group consisting of Gly. The method of claim 5, The amino acid sequence comprises at least one residue selected from the group consisting of Pro in position 251, Glu in position 254, Ile in position 259, Gly in position 277, Glu in position 291 and Gly in position 299 Isolated polypeptide. The method of claim 1, An isolated polypeptide having at least 1.5-fold acyltransferase activity of a wild-type Penicillium chrysogenum acyltransferase corresponding to SEQ ID NO: 54. The method of claim 8, An isolated polypeptide having at least two-fold acyltransferase activity of wild-type penicillium chrysogenum acyltransferase corresponding to SEQ ID NO: 54. The method of claim 8, Wild-type penicillium corresponding to SEQ ID NO: 54 An isolated polypeptide having at least 5-fold acyltransferase activity of chrysogenum acyltransferase. An isolated polypeptide comprising a fragment of an acyltransferase polypeptide selected from the group consisting of: (a) a polypeptide having an amino acid sequence that is at least 96% identical to SEQ ID NO: 38; (b) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 42; (c) a polypeptide having an amino acid sequence that is at least 95% identical to SEQ ID NO: 52; (d) has an amino acid sequence of 96% or more identical to SEQ ID NO: 54, Arg at position 97, Val at position 221, Pro at position 251, Glu at position 254, Ile at position 259, and position 277 A polypeptide having one or more residues selected from the group consisting of Gly, Glu at position 291 and Gly at position 299; And (e) A polypeptide encoded by a polynucleotide that hybridizes under stringent conditions on a substantially full length nucleotide sequence that encodes an amino acid sequence selected from the group consisting of SEQ ID NO: 38 and SEQ ID NO: 42 and SEQ ID NO: 52. A polynucleotide encoding the polypeptide of claim 1. An expression cassette comprising the polynucleotide of claim 15 operably linked to a promoter. A host cell transformed to express the polynucleotide of claim 15. Culturing a host cell containing a nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide under conditions favorable for the production of the polypeptide; Optionally, recovering the polypeptide A method for producing the acyltransferase polypeptide of claim 1 comprising a. Culturing a host cell capable of producing a beta-lactam compound, wherein the host cell contains a nucleic acid construct comprising a nucleic acid sequence encoding the acyltransferase polypeptide of claim 1 under conditions favorable for the production of the beta-lactam compound; Recovering the beta-lactam compound Comprising, the beta-lactam compound. The method of claim 20, N-deacylating the beta-lactam compound and recovering the N-deacylated beta-lactam compound. The method of claim 20, And said beta-lactam compound is adipoyl-7-ADCA.

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