CN101142312A - Altered isopenicillin-n acyltransferase polypeptides and related polynucleotides - Google Patents

Altered isopenicillin-n acyltransferase polypeptides and related polynucleotides Download PDF

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CN101142312A
CN101142312A CNA2006800061244A CN200680006124A CN101142312A CN 101142312 A CN101142312 A CN 101142312A CN A2006800061244 A CNA2006800061244 A CN A2006800061244A CN 200680006124 A CN200680006124 A CN 200680006124A CN 101142312 A CN101142312 A CN 101142312A
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鲁洛夫·阿利·兰斯·伯韦比格
安克·克里伯
安东尼·考克斯
查尔琳·图拉琴
马修·图斌
斯蒂芬·J·詹尼
永红·陈
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Abstract

The present invention relates to novel acyltransferase polypeptides, as well as the polynucleotides that encode them, that are particularly useful in the production of beta-lactam intermediates and antibiotic compounds having adipoyl side chains. The present invention is also directed to related vectors, host cells, and methods for making and using the novel acyltransferase polypeptides.

Description

The isopenicillin-n acyltransferase polypeptides and the related polynucleotides that change
Invention field
The present invention relates generally to can be used for making the novel acyl transferring enzyme polypeptide of beta-lactam intermediate and Antibiotique composition.
Background technology
Of paramount importance classification is penicillin (penams) and cynnematin (ceph-3-ems) in the beta-lactam antibiotics.Penicillin is only made (Penicillium chrysogenum or Aspergillus nidulans) by filamentous fungus, and cynnematin is made by filamentous fungus (Acremoniumchrysogenum) and bacterium (for example Streptomyces clavuligerus).All beta-lactams are shared the common structure feature of quaternary beta-lactam nucleus.Naturally occurring penam is synthetic from three identical seed amino acid precursors with cynnematin: L-α-An Jijiersuan (L-alpha-AAA), L-halfcystine and L-Xie Ansuan.The first two steps of penicillin and cynnematin biosynthetic pathway are identical.In the first step, be tripeptides δ-L-alpha-amino group adipyl-L-cysteamine acyl group-D-Xie Ansuan (ACV) with the amino acid precursor condensation.Necessary reaction cycle (for example discern, activation and peptide bond form) is by multifunctional enzyme ACV synthetic enzyme (ACVS) catalysis.In second step, the linear tripeptides by the closed cyclisation of oxidation ring produces causes the formation of twin nuclei, and promptly quaternary beta-lactam nucleus and five yuan of thiazolidine rings (it is the feature of all penam) merge.Cyclisation is by isopenicillin N synthase (IPNS) catalysis.The compounds represented that produces first biological activity intermediate, it is called as isopenicillin N (IPN).The general description consulted for example EP 0 422 790 (Miller et al.).
After IPN is synthetic, produce the biosynthetic pathway generation difference of penicillin and cynnematin.In some fungies (as in P.chrysogenum and A.nidulans), wetting ability L-α-AAA side chain of IPN can be replaced in the 3rd step and last exchange step by hydrophobic acyl.This side chain can be (for example saccharinic acid or the octylenic acid) in source in the cell or be provided by external source.Penicillin with only direct fermentation of industrial significance is penicillin v and penicillin G, and they are respectively by adding the manufacturings of external source side chain precursor phenylium or toluylic acid.This changes by acetyl-CoA (CoA): by isopenicillin N acyltransferase (AT) catalysis of penDE genes encoding.
On the contrary, cynnematin is made by following method: with the IPN epimerase L-α-AAA side chain isomerism of IPN is turned to the D enantiomorph, produce vitamin N (it is the microbiotic precursor that comprises the cynnematin core).For example in fungi A.crhrysogenum, the α-An Jijiersuan side chain of IPN is produced penicillin N by isomerization, five of penicillin yuan of thiazolidine rings obtain desacetoxy cephalosporin (DAOC) by desacetoxy cynnematin synthetic enzyme (DAOCS) ring enlargement enzyme activity " expansion " afterwards, and it comprises the distinctive hexa-atomic Dihydrotriazine ring of cynnematin.
Many so-called natural beta-lactams (for example penicillin-f, isopenicillin N, cephalosporin etc.) have limitation as antibiotic purposes, because their instabilities, be difficult to from fermented liquid purifying, limited microbiotic effect and/or yield poorly only.Replace the side chain of these beta-lactams make to form semisynthetic penicillin and cynnematin with other side chain, for example amoxycilline Trihydrate bp, penbritin and Cephalexin Monohydrate Micro/Compacted, they are stable more, more easily separated and have a higher antibiotic activity.
Huge diversity with side chain of finding in the 'beta '-lactam compounds of commercial significance make to obtain to be used for synthetic multiple 'beta '-lactam compounds key intermediate the preparation method is even more important more economically and effectively.
Cephalosporin intermediate 7-ADCA is the important intermediate that is used for synthetic many semi-synthetic cynnematins.It is by chemically derived vitamin(e) G or by making as EP 0532341 described bioprocess at present.
In EP 0532341, show: by Penicillium chrysogenum bacterial strain being modified expressing ring enlargement enzyme, and replenish, form adipyl-7-ADCA with the side chain precursor hexanodioic acid.Remove the adipyl side chain with suitable enzyme subsequently, form 7-ADCA.Although the AT endonuclease capable is accepted other side chain except phenyl or phenylium, can the ability that unpredictable AT enzyme is accepted other side chain except that phenyl or phenylium be promoted, or can its substrate specificity be modified.
Legend
Fig. 1 is the synoptic diagram of the carrier (pET-penDE Pc) of 6343bp, and described carrier contains T7 promotor, T7 terminator and kalamycin resistance gene.
Fig. 2 has provided the comparison of acyltransferase aminoacid sequence disclosed herein to be showed.
Summary of the invention
The invention provides the polypeptide with acyltransferase activity, it compares the substrate specificity with change with natural Penicilliumchrysogenum acyltransferase.Particularly, the invention provides compare with natural P.chrysogenum acyltransferase have a raising form the ability of adipyl-6-APA from alpha-amino group adipyl-6-APA.More specifically, the ability of described raising is at least 1.5 times of natural P.chrysogenum acyltransferase ability, preferably at least 2 times, and more preferably at least 5 times, more preferably at least 10 times, most preferably at least 30 times.
The present invention relates to the isolated polypeptide with acyltransferase activity on the other hand, and wherein isolated polypeptide is selected from:
(a) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:38 at least 96%;
(b) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:42 at least 95%;
(c) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:52 at least 95%;
(d) has the polypeptide that the aminoacid sequence identical with SEQ ID NO:54 at least 96% and having is selected from least one residue of the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes; With
(e) by the polypeptide of following polynucleotide encoding, described polynucleotide can be hybridized the described nucleotide sequence coded aminoacid sequence that is selected from SEQ ID NO:38 and SEQ ID NO:42 and SEQ ID NO:52 with the whole substantially length of following nucleotide sequence under stringent condition.
On the other hand, the present invention relates to the to encode polynucleotide of isopenicillin-n acyltransferase polypeptides.
In other embodiments, the present invention relates to expression vector, host cell and method, they can be used for using isopenicillin-n acyltransferase polypeptides of the present invention to make 'beta '-lactam compounds.
Detailed Description Of The Invention
First aspect present invention provides novel acyltransferase (" AT ") the active polypeptide of having, and it is selected from:
The present invention relates to the isolated polypeptide with acyltransferase activity on the other hand, and wherein isolated polypeptide is selected from:
(a) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:38 at least 96%;
(b) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:42 at least 95%;
(c) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:52 at least 95%;
(d) has the polypeptide that the aminoacid sequence identical with SEQ ID NO:54 at least 96% and having is selected from least one residue of the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes; With
(e) by the polypeptide of following polynucleotide encoding, described polynucleotide can be hybridized the described nucleotide sequence coded aminoacid sequence that is selected from SEQ ID NO:38 and SEQ ID NO:42 and SEQ ID NO:52 with the whole substantially length of following nucleotide sequence under stringent condition.
Isopenicillin-n acyltransferase polypeptides of the present invention can be used for making 'beta '-lactam compounds, for example has the penicillin Antibiotique composition of adipyl side chain.Term used herein " isopenicillin-n acyltransferase polypeptides ", " AT polypeptide ", " acyltransferase " and " AT enzyme " commutative in this article use, they refer to show the polypeptide of acyltransferase activity.Term used herein " acyltransferase activity " refer to enzyme at least catalysis 6-amino-penicillanic acid (" 6-APA ") and adipyl-coenzyme A (" Ad-CoA ") to the ability of quantity with the conversion of the detectable adipyl-6-amino-penicillanic acids of embodiment 3 described assay methods (" Ad-6-APA ").Ad-6-APA can be converted into the penicillin with adipyl side chain or the useful as intermediates of 6-APA core.Perhaps as described in the EP 0532341 for example, ad-6-APA is the useful intermediates that can expand as adipyl-7-ADCA.The AT enzyme can also catalysis IPN hydrolysis, by this with α-AAA side chain excision, thereby form 6-APA.Term " acyltransferase polynucleotide " and " AT polynucleotide " are in the commutative use of this paper, the polynucleotide of the isopenicillin-n acyltransferase polypeptides that refers to encode.It is to be understood that mentioning adipyl should not be considered to restriction, and only be the means that characterize at least a catalytic capability of enzyme.
This paper uses term " isolating " to be meant that polynucleotide or polypeptide are not contained in therewith found usually other material of occurring in nature in fact, thereby and does not contain naturally occurring other cell material and substratum in fact.For example " isolated polypeptide " mainly do not contain the sequence of polynucleotide at its natural gene group position flank.
Term " polynucleotide ", " nucleic acid " and " nucleic acid molecule " are used interchangeably in this article, are meant its analogue of DNA, RNA or synthetic.This paper uses term " polypeptide " and " protein " to be used interchangeably, and is meant amino acid whose polymer.
Term " identity per-cent " and " % identity " are used interchangeably at this paper, be meant with Clustal W analysis and (can derive from European bioinformatics Institute, Cambridge, W 1.8 versions of UK) the amino acid sequence identity per-cent that obtains, the quantity that wherein contrasts the centering identical match is technology in addition, and with the quantity of this identical match length divided by reference sequences, and use following acquiescence Clustal W parameter finish slowly/pairing comparison accurately obtains, described parameter is: the open point penalty of breach: 10; Breach expansion point penalty: 0.10; Protein weight matrix: Gonnet series; DNA weight matrix: IUB; Toggle is pairing comparison=SLOW or FULL comparison slowly/fast.
The present invention also provides following isopenicillin-n acyltransferase polypeptides; it is identical with SEQ ID NO:38 at least 97% that described isopenicillin-n acyltransferase polypeptides has; typically identical aminoacid sequence with SEQ ID NO:38 at least 98%; and the aminoacid sequence identical in some embodiments, with SEQ ID NO:38 at least 99%.In another embodiment, the invention provides following acyltransferase, described acyltransferase has the aminoacid sequence identical with SEQ ID NO:52 at least 96%.The present invention includes the AT polypeptide, it is identical with SEQ ID NO:52 at least 97% that described AT polypeptide has, and the aminoacid sequence identical with SEQ ID NO:52 at least 98% and at least 99%.
In another embodiment; the invention provides following isopenicillin-n acyltransferase polypeptides; described isopenicillin-n acyltransferase polypeptides have with from the wild-type acyltransferase (SEQ ID NO:54) of P.chrysogenum; at least 96% identical aminoacid sequence; and have the residue that at least one is selected from following group in its sequence, described group by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th glycine constitute.In certain embodiments, the AT amino acid sequence of polypeptide has at least two residues that are selected from this group, and have at least four residues that are selected from this group sometimes, usually about six residues of as many as (for example the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th glycine).Amino acid position described herein is: based on the best comparison of AT peptide sequence and SEQ IDNO:54, corresponding to the position of SEQ ID NO:54.
Term " best comparison " is meant and uses Clustal W algorithm (Nucleic Acid Research, 22 (22): the comparison that 4673-4680 (1994) creates.With regard to regard to the aminoacid sequence of the best comparison of reference sequences, amino-acid residue " corresponding to " in comparison with this residue paired reference sequences in the position.
In another embodiment of the present invention; isopenicillin-n acyltransferase polypeptides of the present invention is provided; when measuring in embodiment 3 described mensuration, described isopenicillin-n acyltransferase polypeptides has than the high acyltransferase activity of wild-type P.chrysogenum acyltransferase (SEQ ID NO:54).It is acyltransferase activity at least 1.6 times of P.chrysogenum wild-type acyltransferases (SEQID NO:54) that some isopenicillin-n acyltransferase polypeptides of the present invention demonstrates.In special embodiment; isopenicillin-n acyltransferase polypeptides of the present invention demonstrates the about 2 times acyltransferase activity that is at least P.chrysogenum wild-type acyltransferase (SEQID NO:54); sometimes at least about 5 times; in some embodiments, at least about 10 times or 20 times of acyltransferase activities that as many as is about 30 times to 50 times.
In another embodiment; the invention provides the acyltransferase by following polynucleotide encoding, the nucleotide sequence that described polynucleotide can be under stringent condition be selected from the aminoacid sequence of SEQ ID NO:38 and SEQ IDNO:52 with coding is hybridized on whole length substantially.
The term that this paper uses in the context of nucleic acid hybridization experiment (for example Southern and Northern hybridization) " strict hybridization and/or wash conditions " is a sequence dependent, and different under different environmental parameters.The visible Tijessen of extensive guide (1993) of nucleic acid hybridization " LaboratoryTechniques in Biochemistry and Molecular Biology-Hybridization with NucleicAcid Probes; " Part I, Chapter 2 (Elsevier, New York).
With regard to purpose of the present invention, " high strict degree " hybridization and/or wash conditions are selected as usually: low about 5 ℃ or still less (as mentioned below, high strict degree condition also can be mentioned with comparative term) of the heat fusion joint (Tm) of particular sequence during than given ionic strength and pH.Tm is the temperature (under given ionic strength and pH) of the probe hybridization of 50% cycle tests and Perfect Matchings.Very Yan Ge condition is selected as equaling the Tm at particular probe.
Can rule of thumb easily determine at the strictness of any nucleic acid and highly strict hybridization and wash conditions.When for example determining highly strict hybridization and wash conditions, hybridization and wash conditions strengthen gradually (for example by improve temperature, reduce salt concn, improve detergent concentration and/or raising hybridize or wash in the concentration of organic solvent such as methane amide) up to satisfying selected standard.
In Southern or the northern hybridization trace, have the stringent hybridization condition of hybridizing more than the complementary nucleotide of 100 complementary residues on the filter paper to be: under 42 ℃, contain 50% methane amide of 1mg heparin, hybridization is spent the night.An example of strict wash conditions is for (consulting Sambrook in 15 minutes with 0.2x SSC washing down at 65 ℃, et al., " Molecular Cloning-A Laboratory Manual " (1989) Cold Spring Harbor laboratory (Cold Spring Harbor, New York) is to the description of SSC damping fluid).The example of low strict degree washing is 40 ℃ of following 2x SSC washings 15 minutes.
The specific isopenicillin-n acyltransferase polypeptides of the present invention comprises the acyltransferase with following aminoacid sequence, and described aminoacid sequence is corresponding to SEQ ID NOS:2 (H1), 4 (H2), 6 (H3), 8 (H4), 10 (H5), 12 (H6), 14 (H7), 16 (H14), 18 (H25), 20 (H26), 22 (H27), 24 (H28), 26 (H29), 28 (H30), 30 (H31), 32 (H32), 34 (H33), 36 (H34), 38 (H35), 40 (H36), 42 (H37), 44 (H38), 46 (H40), 48 (H41), 50 (H42) and 52 (H43).The acyltransferase activity of these polypeptide is described in embodiment 3.It is the isopenicillin-n acyltransferase polypeptides of these variant polypeptides that the present invention also provides, and it comprises a replacement to six amino-acid residues, disappearance and/or insertion.
Term used herein " variant " is meant the sequence that high per-cent identity is arranged with reference sequences.Variant isopenicillin-n acyltransferase polypeptides of the present invention can be naturally occurring or non-natural exists.The variant isopenicillin-n acyltransferase polypeptides can have one to six amino-acid residue to replacing, lack and/or inserting.This class replaces, disappearance and/or insert can be in polypeptide more than taking place on the site, and can on one or more sites of the N of polypeptide end and/or C end and polypeptide inside, take place.The variant polypeptide that the present invention comprises has acyltransferase activity.
Conservative variant can easily be made by making conservative replacement, the replacement in described replacement such as basic aminoacids (arginine, Methionin and Histidine), acidic amino acid (L-glutamic acid and aspartic acid), polare Aminosaeren (glutamine and l-asparagine), hydrophobic amino acid (leucine, Isoleucine and Xie Ansuan), die aromatischen Aminosaeuren (glycine, L-Ala, Serine, Threonine, proline(Pro), halfcystine and the methionine(Met)) group.
Isopenicillin-n acyltransferase polypeptides variant of the present invention also can use known mutagenesis of this area routine techniques personnel and directed evolution method to make.Can screen the library of creating, obtain having the variant of the acyltransferase activity that uses embodiment 3 described assay methods measurements by these class methods.
In another embodiment, the present invention relates to the fragment of AT polypeptide of the present invention, described fragment shows the AT activity in embodiment 3 described mensuration.Can use commercially available exonuclease and endonuclease easily to prepare fragment according to currently known methods.Term used herein " fragment " is meant the polypeptide with 1 to 15 amino-acid residue disappearance of carboxyl and/or aminoterminal one or both ends.In specific embodiment, this disappearance is 1 to 10 amino-acid residue, is 1 to 5 amino-acid residue in some cases.When using the described assay method of embodiment 3, AT fragment of the present invention has the AT activity of at least 1.6 times of wild-type P.chrysogenum AT activity.In some embodiments, in embodiment 3 described assay methods, AT fragment of the present invention has at least 2 times of wild-type P.chrysogenum AT activity, typically up to about 30 times AT activity.
Second aspect present invention provides the polynucleotide of code book invention isopenicillin-n acyltransferase polypeptides.In one embodiment, the invention provides the isolating polynucleotide that coding has the polypeptide of acyltransferase activity, wherein said isolated polypeptide is selected from:
(a) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:38 at least 96%;
(b) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:42 at least 95%;
(c) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:52 at least 95%;
(d) has the polypeptide that the aminoacid sequence identical with SEQ ID NO:54 at least 96% and having is selected from least one residue of the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes; With
(e) by the polypeptide of following polynucleotide encoding, described polynucleotide can be hybridized the described nucleotide sequence coded aminoacid sequence that is selected from SEQ ID NO:38 and SEQ ID NO:42 and SEQ ID NO:52 with the whole substantially length of following nucleotide sequence under stringent condition.
Specific acyltransferase polynucleotide of the present invention comprise the polynucleotide with following polynucleotide sequence, and described polynucleotide sequence is corresponding to SEQ ID NO:1 (H1), 3 (H2), 5 (H3), 7 (H4), 9 (H5), 11 (H6), 13 (H7), 15 (H14), 17 (H25), 19 (H26), 21 (H27), 23 (H28), 25 (H29), 27 (H30), 29 (H31), 31 (H32), 33 (H33), 35 (H34), 37 (H35), 39 (H36), 41 (H37), 43 (H38), 45 (H40), 47 (H41), 50 (H41) and 51 (H43).SEQ ID NOS:13 and 15 is polynucleotide variants of the identical isopenicillin-n acyltransferase polypeptides of coding (being that SEQ ID NOS:14 is identical with 16).This area the routine techniques personnel will easily understand: owing to the degeneracy of genetic codon, have the diversity of the nucleotide sequence of code book invention isopenicillin-n acyltransferase polypeptides, this sublist that can access to your password is easily determined.
Those skilled in the art will appreciate that: can be by each codon in the standard technique modification of nucleic acids (except ATG, it typically is unique password of methionine(Met), and TGG, it typically is unique password of tryptophane) with polypeptide identical on the encoding function.Therefore, any described sequence has all implied each silent mutation of nucleic acid encoding.Can make it meet the suitableeest codon selection in the purpose host living beings by modifying polynucleotide, acyltransferase polynucleotide codon is optimized, be used for expressing at concrete host living beings.This area the routine techniques personnel will appreciate that: provide form and other bibliography at the preference information of multiple biology to be easy to obtain.
Polynucleotide of the present invention can use method preparation well known in the art.Consult for example Carruthers, et al., Cold Spring Harbor Symp.Quant.Biol., 47:411-418 (1982) and Adams, et al., J.Am.Chem.Soc., 105:661 (1983).Typically, the synthetic respectively oligonucleotide up to about 100 bases connects together the formation aim sequence by enzymatic or chemical process then.
The third aspect the invention provides the expression cassette that contains acyltransferase polynucleotide of the present invention, and described polynucleotide are operably connected with adjusting sequence such as promotor.Expression cassette of the present invention provides the heredity of acyltransferase polynucleotide to shift, and expresses and make the isopenicillin-n acyltransferase polypeptides of their codings.The adjusting sequence of construct acts on transcribing and translating of control acyltransferase polynucleotide.
Term used herein " construct " in this article refers to by DNA, RNA, its synthetic analogues or two or more strand that constitutes or double chain acid molecule in these.Except that expression cassette, nucleic acid construct can randomly contain other nucleic acid fragment, and described nucleic acid fragment can provide other useful function in heredity is shifted and/or expressed, but selectable marker gene for example.
Nucleic acid construct can comprise bacterium, fungi and plant from a large amount of different sources.Suitable originated from fungus comprises Aspergillus and Penicillium (for example Penicillium chrysogenum).Nucleic acid construct can also be the carrier format that is applicable to operation, transforms and/or express acyltransferase polynucleotide of the present invention.For example, can select and/or the designing nucleic acid construct according to its feature the purpose host living beings that is introduced into.Therefore, but the construct self-replicating, and promptly it can be used as the outer entity of karyomit(e) such as plasmid exists usually.In another embodiment, nucleic acid construct can be to be integrated into genomic molecule after introducing host cell.
Putting into practice the suitable adjustable sequence of using when of the present invention can be to show any polynucleotide of suitable active in the purpose host cell, and can be natural or synthetic source.The promotor that is applicable to a fungal host cells comprises for example Aspergillus nidulans trpC promotor (Yelton, M.M., etal., Proc.Natl.Acad.Sci.USA, 81:1470-1474 (1984); Mullaney, E.J.et al., Mol.Gen.Genet., 199:37-45 (1985)), Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promotor (Punt, P.J., et al., Gene, 56 (1): 117-24 (1987); Punt, P.J., et al., Gene, 49-57 (1988)), Aspergillus nidulans ADH3 promotor (McKnight et al. 69 (1):, EMBO J., 4:2093-2099 (1985)), Aspergillus awamori glucoamylase gene promotor (Nunberg, J.H., et al., Mol.Cell Biol., 2306-15 (1984)) and Aspergillusoryzae transcriptional elongation factor gene promoter (Kitamoto, N., et al. 4 (11):, Appl.Microbiol.Biotechnol., 50 (1): 85-92 (1998).Other suitable promotor comprises the promotor from penicillin biosynthetic pathway self, Penicillium chrysogenum pcbC (IPNS) promotor (Barredo for example, J.L., et al., 91-98 (1989)) or Penicilliumchrysogenum penDE (AT) promotor (Diez Mol.Gen.Genet., 216 (1):, B., et al., Mol.Gen.Genet., 218 (3): 572-6 (1989)).When needs manufacturing during, can advantageously use same or analogous promotor to regulate the synchronous generation of polypeptide more than a peptide species.For example, when in the host cell that a plurality of polynucleotide need be transformed the enzyme that coding beta-lactam antibiotics biosynthetic pathway into uses, carry out simultaneously may wanting to promote production efficiency to the production of intermediate in this approach.
But nucleic acid construct of the present invention also can comprise selective marker, the polynucleotide of coded markings for example, and described mark is convenient to select to contain the cell of this nucleic acid construct.This area routine techniques personnel can understand that but many selective markers are fit to us and are used for expression vector of the present invention.Other gene that the suitable marks packets selected is drawn together kalamycin resistance gene, chloramphenicol resistance gene and given antibiotics resistance.Be used to have the marks packets of preferably can selecting of a fungi to draw together coding acetamidase (Corrick, C.M.etal., Gene, 63-71 (1987)), hygromycin resistance (Kaster, K.R., et al. 53 (1):, Nucleic AcidsRes., 11 (19): 6895-911 (1983); Punt, P.J., et al., Gene, 11724 (1987)), phleomycin resistance (Drocourt, D., et al. 56 (1):, Nucleic Acids Res., 4009 (1990)), F-1991 (benomyl) resistance (Orbach, M.J., et al. 18 (13):, Mol.Cell.Biol., 6 (7): the gene of 2452-61 (1986).Other gene that can be used as selective marker is: coding is used for the gene of auxotrophy complementary factor, PyrG (Cantoral for example, J.M., et al., Nucleic Acids Res., 8177 (1988)), ArgB (Upshall, A. 16 (16):, et al., 349-54 (1986)), niaD (Johnstone, I.L. Mol.Gen.Genet., 204 (2):, et al., Gene, 181-92 (1990)) and TrpC (Penalva, M.A. 90 (2):, Nucleic Acids Res., 15 (4): 1874 (1987)).In the penicillin kind, when the purpose reaction was made for beta-lactam, the nucleic acid construct purposes that contains ampicillin resistance gene was less, because this genes encoding β-Nei Xiananmei, can the degraded beta-lactam after it is expressed.Replace, when the purpose reaction is that phleomycin resistant gene marks such as (phlR) can be used in the penicillin kind when making beta-lactam.
Nucleic acid construct of the present invention also can contain target signal sequence or secretory signal sequence, sets out in the ferment substratum with the expression of instructing isopenicillin-n acyltransferase polypeptides purpose site in host cell or expression.Target sequence and/or secretory signal sequence are added in the acyltransferase polynucleotide, make it all be arranged in identical reading frame.These signal sequences can be that host cell is natural, or they can be synthetic or the external source source.When host living beings when thread fungi is arranged, signal peptide can be from fungal enzyme, as Aspergillus spp. amylase.Be used for making up have desired characteristic (comprise and handle multiple nucleotide fragments and comparison reading frame etc.) construct be operating as well known to those skilled in the art.Consult for example Sambrook, et al. (1989), " Molecular Cloning:A Laboratory Manual, " 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
The host cell that construct is introduced into can be any cell with desired characteristic.For example it can be the cell that can make a large amount of isopenicillin-n acyltransferase polypeptides of the present invention.It can also be the host cell that can produce 'beta '-lactam compounds, promptly contains the host cell of the mechanism of making 'beta '-lactam compounds.In some embodiments, host cell is bacterium, yeast or a fungi is arranged.It can be for example Aspergillus spp. or Penicillium chyrsogenum that a fungi is arranged.The purposes that Aspergillus spp. is used for marking protein is described in for example EP 272 277, EP 238 023 and EP 184 438.The purposes that genetically modified P.chrysogenum bacterial strain is used to make adipyl-7-ADCA is described in for example EP 532 341.
Under the condition that allows expression of polypeptides, in suitable nutritional medium, cultivate the transformed host cells that contains construct, recyclable after this polypeptide.Can from substratum, separate host cell by centrifugal or filtration and finish recovery.When polypeptide produces in cell, by centrifugal or filter and from fermention medium, to reclaim cell, and with cell homogenates to discharge polypeptide.Carry out purifying then by salt (for example ammonium sulfate) means precipitating proteins component from substratum or supernatant liquor, and by multiple chromatogram and/or electrophoresis process (for example ion-exchange chromatography, gel filtration chromatography, isoelectrofocusing, nucleophilic chromatogram etc.).Recovery technology depends on the character of specific polypeptide usually.Polypeptide can be used further then.
Perhaps or extraly, in suitable nutritional medium, under the condition that allows the purpose beta lactam compounds to express, cultivate the transformed host cells that contains construct of the present invention.
Fourth aspect present invention provides the method that is used to prepare 'beta '-lactam compounds, and described method comprises: cultivate the transformed host cells that contains construct of the present invention under the condition of the beta lactam compounds that allows generation N-acylations; Randomly the 'beta '-lactam compounds deacylation with the N-acylations that produces produces corresponding N-deacylation compound; With recovery N-'beta '-lactam compounds acylations or the N-deacylation.The technician can discern the character of the 'beta '-lactam compounds of generation at once according to the transformed host cells of using.If contain the host transformed of construct of the present invention is genetically modified P.chrysogenum bacterial strain (it is modified to express ring enlargement enzyme), and then N-deacylated tRNA base 'beta '-lactam compounds is 7-ADCA.Perhaps, if contain the Acremonium chrysogenum bacterial strain that the transformed host cells of construct of the present invention is natural generation ring enlargement enzyme, N-deacylated tRNA base 'beta '-lactam compounds is 7-ACA so.
The N-acyl side-chain precursor that carries out using in the method in following of the condition of the 'beta '-lactam compounds that helps to make the N-acylations is preferably hexanodioic acid or its salt.Perhaps can use other side chain precursor, for example the precursor described in International Application No. WO 95/04148, WO95/04149, WO98/48034 or the WO 98/48035.
The required technology of above-mentioned array of carriers and transformed host cells that produces is familiar with by those skilled in the art, and all these class embodiments are included in the scope of the present invention.During the preparation expression cassette, can handle multiple polynucleotide element it is in suitable direction and the suitable reading frame.Can use fit (adapter) or joint to connect the polynucleotide element for this reason.Similarly, can use multiple currently known methods to introduce restriction site easily, remove unnecessary Nucleotide, remove restriction site etc.
Polynucleotide sequence of the present invention can be used for identifying and/or separating corresponding sequence from other biology (for example other fungi).Can use methods known in the art such as PCR, hybridization etc., according to identifying sequence, and be also contained among the present invention according to the isolating sequence of sequence identity with the open sequence of this paper with the sequence identity degree of sequence of the present invention.The appropriate method that is used for this class evaluation is known in the art, and be described in for example Sambrook et al. (1989) Molecular Cloning:ALaboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Plainview, New York) in.Nucleotide sequence fragment for example disclosed herein can be used as probe.This class probe can with corresponding nucleotide sequence and messenger RNA(mRNA) specific hybridization, for example, be used for measuring the single nucleotide polymorphisms of the different candidate sequences of difference at SSCP.In order to be issued to specific hybridization in multiple condition, this class probe comprises the sequence of difference between the candidate sequence.This class probe can be used for from selected biology by the corresponding acyltransferase sequence of pcr amplification; This technology can be used for from biology separating other sequence or as the diagnostic assay method to determine existing or expression level of encoding sequence in the biology.
Provide following embodiment to be used for restriction, but be used for explanation.
Embodiment 1
Clone and expression acyl transferase gene
Acyl-CoA: isopenicillin N acyltransferase (acyltransferase) is identified in some product penicillin biologies: Penicillium chrysogenum ATCC9480 (Tobin et al.1990; J.Bacteriol.172 (10): 5908); Aspergillus nidulans ATCC38163 (Tobin et al.1990; J.Bacteriol.172 (10): 5908); Penicillium nalgiovense ATCC10472 (Laichet al.1999; 1236) and Penicillium griseofulvumATCC10120 (Laich et al.2002, Appl.And Env.Micorbiol.68 (3): 1211) Appl.And Env.Micorbiol.65 (3):.Can by cultivate biology, extract total RNA, purified mRNA and use ThermoScript II construction cDNA library clone this genoid.The standard operation of dna technique is described in for example Sambrook et al. (Sambrook, J., Fritsch, E.F.and Maniatis, T.:Molecular Cloning.A LaboratoryManual, 2nd ed.Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).The standard conditions of E.coli strain growth and processing are described (Miller, J.:Experiments in Molecular Genetics.Cold Spring Harbor Laboratory Press, ColdSpring Harbor, NY, 1972) by Miller.
Can come isolated genes by designing and synthesizing with acyl transferase gene 5 ' and 3 ' end annealed PCR primer, from cDNA amplified library specific gene and expression vector, clone.Perhaps described can be from the method for the initial synthetic known array acyltransferase of short oligonucleotide for present embodiment.The synthetic of this class synthetic gene extensively described (Stemmer WP et al.1995, Gene 164:49; Jayaraman K et al.1989, Nucleic Acids Res.17 (11): 4403; Holowachuk EW etal.1995, PCR Methods Appl.4 (5): 299; Prodromou C et al.1992, Protein Eng.5 (8): 827).
For example; for synthetic acyltransferase (penDE) from Penicillium chrysogenum ATCC 9480; synthetic following overlapping oligonucleotide; the two strands of its common coding penDE gene; and 5 ' and 3 ' end sequence (the USA-QIAGEN Inc. of the commercially available obtainable restriction endonuclease recognition site of encoding; Valencia, CA, USA):
oligo：1?tctagacatatgcttcacatcctctgtcaaggcactccc(SEQ?ID?NO：57)
oligo：2 tttgaaatcggctacgaacatggctctgctgccaaagcc(SEQ?ID?NO：58)
oligo：3 gtgatagccagaagcattgacttcgccgtcgatctcatccga(SEQ?ID?NO：59)
oligo：4 gggaaaacgaagaagacggacgaagagcttaaacaggta(SEQ?ID?NO：60)
oligo：5 ctctcgcaactggggcgcgtgatcgaggaaagatggccc(SEQ?ID?NO：61)
oligo：6 aaatactacgaggagattcgcggtattgcaaagggcgctgaa(SEQ?ID?NO：62)
oligo：7 cgcgatgtctccgagattgtcatgcttaatacccgcacg(SEQ?ID?NO：63)
oligo：8 gaatttgcatacgggctcaaggcagcccgtgatggctgc(SEQ?ID?NO：64)
oligo：9 accactgcctattgtcaacttccaaatggagccctccagggc(SEQ?ID?NO：65)
oligo：10 caaaactgggatttcttttctgccaccaaagagaacctg(SEQ?ID?NO：66)
oligo：11 atccggttaacgatccgtcaggccggacttcccaccatc(SEQ?ID?NO：67)
oligo：12 aaattcataaccgaggctggaatcatcgggaaggttggattt(SEQ?ID?NO：68)
oligo：13 aacagtgcgggcgtcgccgtcaattacaacgcccttcac(SEQ?ID?NO：69)
oligo：14 cttcagggtcttcgacccaccggagttccttcgcatatt(SEQ?ID?NO：70)
oligo：15 gccctccgcatagcgctcgaaagcacttctccttcccaggcc(SEQ?ID?NO：71)
oligo：16 tatgaccggatcgtggagcaaggcggaatggccgccagc(SEQ?ID?NO：72)
oligo：17 gcttttatcatggtgggcaatgggcacgaggcatttggt(SEQ?ID?NO：73)
oligo：18 ttggaattctcccccaccagcatccgaaagcaggtgctcgac(SEQ?ID?NO：74)
oligo：19 gcgaatggtaggatggtgcacaccaaccactgcttgctt(SEQ?ID?NO：75)
oligo：20 cagcacggcaaaaatgagaaagagctcgatcccttaccg(SEQ?ID?NO：76)
oligo：21 gactcatggaatcgccaccagcgtatggagttcctcctcgac(SEQ?ID?NO：77)
oligo：22 gggttcgacggcaccaaacaggcatttgcccagctctgg(SEQ?ID?NO：78)
oligo：23 gccgacgaagacaattatccctttagcatctgccgcgct(SEQ?ID?NO：79)
oligo：24 tacgaggagggcaagagcagaggcgcgactctgttcaatatc(SEQ?ID?NO：80)
oligo：25 atctacgaccatgcccgtagagaggcaacggtgcggctt(SEQ?ID?NO：81)
oligo：26 ggccggccgaccaaccctgatgagatgtttgtcatgcgg(SEQ?ID?NO：82)
oligo：27 tttgacgaggaggacgagaggtctgcgctcaacgccaggctt(SEQ?ID?NO：83)
oligo：28 atgttcgtagccgatttcaaagggagtgccttgacagaggat(SEQ?ID?NO：84)
oligo：29 gtcaatgcttctggctatcacggctttggcagcagagcc(SEQ?ID?NO：85)
oligo：30 cgtcttcttcgttttccctcggatgagatcgacggcgaa(SEQ?ID?NO：86)
oligo：31 cacgcgccccagttgcgagagtacctgtttaagctcttcgtc(SEQ?ID?NO：87)
oligo：32 gcgaatctcctcgtagtatttgggccatctttcctcgat(SEQ?ID?NO：88)
oligo：33 aatctcggagacatcgcgttcagcgccctttgcaatacc(SEQ?ID?NO：89)
oligo：34 cttgagcccgtatgcaaattccgtgcgggtattaagcatgac(SEQ?ID?NO：90)
oligo：35 aagttgacaataggcagtggtgcagccatcacgggctgc(SEQ?ID?NO：91)
oligo：36 aaagaaatcccagttttggccctggagggctccatttgg(SEQ?ID?NO：92)
oligo：37 ctgacggatcgttaaccggatcaggttctctttggtggcaga(SEQ?ID?NO：93)
oligo：38 tccagcctcggttatgaatttgatggtgggaagtccggc(SEQ?ID?NO：94)
oligo：39 ggcgacgcccgcactgttaaatccaaccttcccgatgat(SEQ?ID?NO：95)
oligo：40 ggtgggtcgaagaccctgaaggtgaagggcgttgtaattgac(SEQ?ID?NO：96)
oligo：41 ttcgagcgctatgcggagggcaatatgcgaaggaactcc(SEQ?ID?NO：97)
oligo：42 ctccacgatccggtcataggcctgggaaggagaagtgct(SEQ?ID?NO：98)
oligo：43 attgcccaccatgataaaagcgctggcggccattccgccttg(SEQ?ID?NO：99)
oligo：44 gctggtgggggagaattccaaaccaaatgcctcgtgccc(SEQ?ID?NO：100)
oligo：45 caccatcctaccattcgcgtcgagcacctgctttcggat(SEQ?ID?NO：101)
oligo：46 tttctcatttttgccgtgctgaagcaagcagtggttggtgtg(SEQ?ID?NO：102)
oligo：47 ctggtggcgattccatgagtccggtaagggatcgagctc(SEQ?ID?NO：103)
oligo：48 tttggtgccgtcgaacccgtcgaggaggaactccatacg(SEQ?ID?NO：104)
oligo：49 gggataattgtcttcgtcggcccagagctgggcaaatgcctg(SEQ?ID?NO：105)
oligo：50 tctgctcttgccctcctcgtaagcgcggcagatgctaaa(SEQ?ID?NO：106)
oligo：51 acgggcatggtcgtagatgatattgaacagagtcgcgcc(SEQ?ID?NO：107)
oligo：52 atcagggttggtcggccggccaagccgcaccgttgcctctct(SEQ?ID?NO：108)
oligo：53 cctctcgtcctcctcgtcaaaccgcatgacaaacatctc(SEQ?ID?NO：109)
oligo：54 cgtcatgaagagGcttcaaagcctggcgttgagcgcaga(SEQ?ID?NO：110)
The homogeneous concentration of oligonucleotide with 250 μ M is mixed.Mixture dilutes 100 times in 20 μ l PCR mixtures, described PCR mixture contains 10mM Tris-HCl pH 9.0/2.2mMMgCl2/50mM KCl/0.1%Triton X-100[1x clone's Pfu damping fluid (Stratagene, LaJolla, CA, USA)]/each deoxynucleotide of 0.2mM (Stratagene, La Jolla, CA, USA)/Taq archaeal dna polymerase (Promega, Madison, the WI of 1u, USA)/the Pfu archaeal dna polymerase (Stratagene of 0.02u, La Jolla, CA, USA).Gene is gone up synthetic being set at the thermal cycler of following program [the small-sized circulation instrument of MJResearch PTC-150 (MJ Research, Inc., Reno, NV, USA)]: 94 ℃ 30 seconds, 52 ℃ of 30 seconds and 72 ℃ 30 seconds, 55 circulations.The gene synthetic mixture that produces dilutes 40 times in 100ul PCR mixture, described PCR mixture contains 10mM Tris-HCl pH 9.0/2.2mM MgCl2/50mM KCl/0.1%Triton X-100[1x clone's Pfu damping fluid (Stratagene, La Jolla, CA, USA)]/each deoxynucleotide (Stratagene of 0.2mM, LaJolla, CA, USA)/Taq archaeal dna polymerase (Promega, Madison, the WI of 5u, USA)/the Pfu archaeal dna polymerase (Stratagene of 0.1u, La Jolla, CA, USA)/concentration is 2 kinds of outside primers of 1 μ M.2 kinds of outside primers can be identical; because these two kinds of oligonucleotide have been represented 5 ' end of normal chain and minus strand, add NdeI recognition site (initiator codon of ATG of half and acyl transferase gene is overlapping behind the recognition sequence) and add the NotI recognition site with the outside primer of normal chain 5 ' end annealed in this embodiment with the outside primer of minus strand 5 ' end annealed.Gene is gone up amplification being set at the thermal cycler of following program [the small-sized circulation instrument of MJ Research PTC-150 (MJ Research, Inc., Reno, NV, USA)]: 94 ℃ 30 seconds, 50 ℃ of 30 seconds and 72 ℃ 60 seconds, 23 circulations.Final gene uses QIAquick PCR purification kit (USA-QIAGEN Inc., Valencia, CA, USA) purifying is with NdeI and NotI (New England Biolabs, Beverly, MA, USA) digestion and use QIAquick gel extraction kit (USA-QIAGEN Inc., Valencia, CA, USA) the 1.0-kb fragment that obtains of purifying.Synthetic can be cloned in the suitable expression vector from the penDE gene of Penicillium chrysogenum ATCC9480.All other acyl transferase genes described herein can use similar method, by introduce to oligonucleotide sequence corresponding change (corresponding to the change of comparing sequence from the penDE gene of Penicillium chrysogenum ATCC9480) make up.
Plasmid pET24B is by Studier (Moffatt, B.A.et al.1986, J.Mol.Biol.189:113) create and by Novagen (Novagen, Inc., Madison, WI, USA) further the exploitation so that in intestinal bacteria one of pET serial carrier of clone, detection and purification of recombinant proteins matter.These carriers have the Colicine E1 replicon of pBR322 usually, and give penbritin or kalamycin resistance.Two kinds of general carrier classification are arranged in the pET series, transcribe and translate.Translation carrier (it contains the efficient ribosome bind site from phage t7 usually) is designed to express the gene that does not have self ribosome bind site.Perhaps, transcription vector is designed to express the target gene that has himself prokaryotic organism ribosome bind site.Clone from the acyl transferase gene of Penicillium chrysogenum ATCC9480 to have set forth how to carry out the expression of active acyl transferring enzyme with plasmid pET24B (translation carrier).The multiple clone site of pET24B (MCS) contains NdeI and NotI recognition sequence, allows that acyltransferase is carried out directed cloning and synthesizes.With NdeI and NotI (New EnglandBiolabs, Beverly, MA, USA) digestion pET24B, and use QIAquick gel extraction kit (USA-QIAGEN Inc., Valencia, CA, USA) purifying, generation suitable expression vector.Use T4DNA ligase enzyme test kit (New England Biolabs, Beverly, MA, USA) the complementary sticky end of two fragments of connection (pET24B carrier and acyltransferase insert fragment).In case the acyltransferase fragment connects into pET24B plasmid; produced the novel plasmid that is called pET-penDE Pc (Fig. 1); then can confirm duplicating and expressing of gene by the following method: recombinant plasmid transformed is advanced e. coli bl21 cell (Novagen; Inc.; Madison; WI, USA) in, express according to the antibiotics resistance gene that carries out in the plasmid then and select cell.With regard to pET24B, use kantlex as the selective marker that contains the cell of the functional plasmid of complete sum, described plasmid portability or do not carry acyl transferase gene.Select a small amount of clone, cultivation and use QIAprep Spin prepare test kit (USA-QIAGEN Inc., Valencia, CA, USA) preparation DNA in a small amount in the liquid medium within.The ABI3700DNA analyser (Applied Biosystems, Inc, Foster City, CA, USA) go up to use the pET sequencing primer (Novagen, Inc., Madison, WI USA) checks order to DNA, and selects to have the clone of correct acyltransferase sequence.Analysis to acyltransferase activity is described among the embodiment 2.
Embodiment 2
Express acyltransferase
Plasmid pET-penDE Pc is transformed theory of evolution competence e. coli bl21 (DE3) cell (Novagen, Inc., Madison, WI, USA) in, and coat and contain 1% glucose (Sigma, St.Louis, MO, USA) and paraxin (30ug/mL) (Sigma, St.Louis, MO, USA) LB medium agar (EM Science, Gibbstown, NJ, USA) on.The independent clone who forms on the picking flat board, and be used to inoculate the 2xYT substratum that contains 0.5% glucose and paraxin (EM Science, Gibbstown, NJ, USA).Containing glucose in the substratum is important for reducing precocious genetic expression, and growth may be deleterious for cell in described precocious genetic expression.In shaking table under 37 ℃ with the culture overnight incubation.The branch styles such as a small amount of of the culture that produces are diluted in the fresh LB substratum that into contains 0.5% glucose and paraxin, to the about 0.05A of cell concn 600, and culture is cultured to early logarithmic phase (0.5A at least 600About cell concn).(MO USA) induces the expression of acyltransferase for Sigma, St.Louis, and culture shaken at 28 ℃ again hatches 16 hours by adding 0.2mM isopropyl-(IPTG).By at 4 ℃ of 4000rpm centrifugal collecting cells, and with the morpholine damping fluid of 5mM pH7.5 (Sigma, St.Louis, MO, USA) washing is at least three times, to remove any salt residue of not expecting.Washed cell is resuspended in 100uL lysis buffer (2mg/mL Polymyxin B sulfate (PMBS) (Amersham Biosciences, Newark, NJ, USA), 10U/ μ l N,O-Diacetylmuramidase (Epicentre, Madison, WI, USA.), 1U/ μ lRNase (Epicentre, Madison, WI is USA.) with 10U/ μ l DNase (Epicentre, Madison, WI, USA.)) in, and at room temperature rock.
Embodiment 3
Analyze acyltransferase activity
Use the assessment of this assay method with 6-APA and Ad-CoA substrate conversion acyltransferase specific activity as the Ad-6-APA product.1.5mM 6-APA, 50mM DTT that prepared fresh is independent and 0.6mM Ad-CoA mother liquor, this carries out among the pH 7.5 by it being dissolved in separately 5mM morpholine damping fluid.6-APA and DTT can derive from Sigma (St.Louis, MO, USA).The adipyl coenzyme A obtains as follows: under nitrogen atmosphere to through stirring evenly and frozen water refrigerative coenzyme A sodium salt (400mg; 0.48mmol) acetone 60ml)+water (0.6ml)+0.2M KHCO3 (being used for improving pH) suspension adds adipyl muriate (0.14ml; 6.95mmol).The pH of conditioned reaction composition is to～7.7 and about 60 minutes of restir.Under reduced pressure partly remove acetone afterwards, be dissolved in the cold water product and lyophilize.Finally product is carried out ultrafiltration.Output is 0.9g.Productive rate is determined as 70%.Handle the water that uses with nitrogen gas stream, prevent the coenzyme A oxidation.
The 0.6mM Ad-CoA of 1.5mM 6-APA, the 4mL of 8mL and the 50mMDTT of 4mL are mixed, make the mother liquor of 0.75mM 6-APA, 0.15mM Ad-CoA, 12.5mM DTT, 5mM morpholine damping fluid (pH7.5).At room temperature will in solution, add the pH 7.55mM morpholine damping fluid of 300 μ L from the lysis of embodiment 2 after 30 minutes.For initial acyltransferase reaction, change the 5mM morpholine damping fluid of 155 μ L in pipe or in the hole of 96 hole flat boards over to.Add the fresh mother liquor of 30 μ L (0.75mM 6-APA, 0.15mM Ad-CoA, 12.5mM DTT, 5mM morpholine damping fluid, pH7.5) and the acyltransferase from whole lysate of 20 μ L 1: 4 dilution.By imbibition 2 to 3 times gently mixed enzyme and mother liquor.Allow reaction at room temperature to continue 3 minutes.60 μ L reaction mixtures are transferred in the new microwell plate or container that contains 60 μ L ice cold methanol, and mixing makes reaction terminating.Preferably with sample filter through for example Whatman customization filter plate (1 micron pore size) (Whatman Inc., Clifton, NJ, USA), and at the bottom of Nunc V-shape the polystyrene micropore titer plate (NY USA) goes up and collects filtrate for Nunc, Rochester.With neat foil sealing flat board.
Can use mass spectroscopy to detect the Ad-6-APA product.Electrospray ionization mass spectrometry (ESI/MS) analysis can (Micromass, Manchester UK) go up the use electrospray ion source and carry out at three grades of quadrupole mass spectrometers of Quattro Ultima.Typically, as described in embodiment 2, sample is dissolved in the methanol (1: 1).Adipyl-6-APA will produce the strong deprotonation of negative ion mode molecule ([M-H]-.Capillary voltage is 3.0kV, and source temperature is 120 ℃, and the desolvation temperature is 250 ℃.Under the collision energy of the awl energy of 15V, 15eV by mass transfer monitoring adipyl-6-APA of 343.0 to 265.0.Can be by sample peak area and typical curve be relatively come quantitative Ad-6-APA.By the injection concentration known the ad-6-APA sample and measure peak area and come the base of calculation curve.
Flow injection (FIA) is analyzed, and HTP pal has been equipped in use, and (Switzerland) (CA) LC system is sent mobile phase with 0.5mL/ minute flow velocity to 1100 series of automatic sampling instrument for Agilent, San Jose for CTC analytics, Zwingen.Injection 5mL sample.Isocratic elution mobile phase in 70% ethanol and 30% water.
In this is measured, all show greater than acyltransferase activity (corresponding to the Ad-6-APA product amount that produces) from the wild-type acyltransferase (SEQ ID NO:54) of Penicillium chrysogenum corresponding to the isopenicillin-n acyltransferase polypeptides of SEQ ID NOS:2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,32,34,36,38,40,42,44,46,48,50 and 52 sequences.
The result provides in table 1:
Table I. with respect to the acyltransferase activity of P.chrysogenum AT (SEQ ID NO:54).
The AT polypeptide The improved living-article multiple of dried relatively P.chrysogenum AT
H01(SEQ?ID?NO：2) *
H02(SEQ?ID?NO：4) **
H03(SEQ?ID?NO：6) **
H04(SEQ?ID?NO：8) **
H05(SEQ?ID?NO：10) *
H06(SEQ?DI?NO：12) *
H07(SEQ?ID?NO：14) **
H25(SEQ?ID?NO：18) **
H26(SEQ?ID?NO：20) **
H27(SEQ?ID?NO：22) **
H28(SEQ?ID?NO：24) **
H29(SEQ?ID?NO：26) **
H30(SEQ?ID?NO：28) **
H31(SEQ?ID?NO：30) **
H32(SEQ?ID?NO：32) **
H34(SEQ?ID?NO：36) **
H35(SEQ?ID?NO：38) ***
H36(SEQ?ID?NO：40) **
H37(SEQ?ID?NO：42) **
H38(SEQ?ID?NO：44) ***
H40(SEQ?ID?NO：46) **
H41(SEQ?ID?NO：48) ***
H42(SEQ?ID?NO：50) ***
H43(SEQ?ID?NO：52) ***
Key: *1＜improvement multiple＜2
*2≤improvement multiple＜10
* *10≤improvement multiple≤30
The level that one of ordinary skill in the art of the present invention are all indicated in all publications mentioned in the specification sheets and patent application.This paper is incorporated in all publications and patent application by reference into, is equivalent to point out that publication or patent application that each is independent are incorporated into especially and individually by reference.Subtitle in the specification document is comprised into this paper so that check the purpose of file, is not to mean the content that limits file by any way.
Although, describe aforementioned invention in detail by the mode of explanation and example, it is evident that some change and modification can put into practice in additional claims scope for the clear purpose of understanding.
Sequence table
＜110〉DSM IP Assets BV
＜120〉isopenicillin-n acyltransferase polypeptides of Gai Bianing and related polynucleotides
<130>24315WO
<140>
<141>
<160>110
<170>FastSEQ?for?Windows?Version?4.0
<210>1
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H1
<221>CDS
<222>(1)...(1074)
<400>1
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gat?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?ValArg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>2
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H1
<400>2
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?lle?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?lle?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>3
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H2
<221>CDS
<222>(1)...(1074)
<400>3
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?cag?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gta?ttt?ggt?ttg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Val?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?cag?cac?ggc?aaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
aaa?gag?ctc?gat?ccc?tta?ccg?gac?tca?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?gac?ggg?ttc?gac?ggc?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gcc?gac?gaa?gac?aat?tat?ccc?ttt?agc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>4
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H2
<400>4
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Val?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>5
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H3
<221>CDS
<222>(1)...(1074)
<400>5
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?cag?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttt?ggt?ttg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?cag?cac?ggc?aaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
aaa?gag?ctc?gat?ccc?tta?ccg?gac?tca?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?gac?ggg?ttc?gac?ggc?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gcc?gac?gaa?gac?aat?tat?ccc?ttt?agc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>6
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H3
<400>6
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>7
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H4
<221>CDS
<222>(1)...(1074)
<400>7
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?caa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Gln
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?acc?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?gac?ggg?ttc?gac?ggc?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>8
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H4
<400>8
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Gln
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>9
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H5
<221>CDS
<222>(1)...(1074)
<400>9
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ttg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ctc?ata?acc?gag?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Leu?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>10
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H5
<400>10
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Leu?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>11
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H6
<221>CDS
<222>(1)...(1074)
<400>11
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gag?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttc?gca?tac?ggc?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggt?tgc?acc?acc?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tcc?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttt?ggt?ttg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>12
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H6
<400>12
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>13
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H7
<221>CDS
<222>(1)...(1074)
<400>13
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gcg?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?cta?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggc?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?ccc 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>14
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H7
<400>14
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>15
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H14
<221>CDS
<222>(1)...(1074)
<400>15
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gag?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttc?gca?tac?ggc?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?caa?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?ValGly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gat?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>16
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H14
<400>16
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>17
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H25
<221>CDS
<222>(1)...(1074)
<400>17
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttc?gca?tac?ggc?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggt?tgc?acc?acc?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cgg 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Arg
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?ccc 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>18
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase 25
<400>18
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Arg
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>19
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H26
<221>CDS
<222>(1)...(1074)
<400>19
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aac 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?gac?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?ccc 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>20
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H26
<400>20
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
ll5 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>21
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H27
<221>CDS
<222>(1)...(1074)
<400>21
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gac?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tcc?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>22
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H27
<400>22
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>23
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H28
<221>CDS
<222>(1)...(1074)
<400>23
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gac?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ctc?ata?acc?gag?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Leu?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?cag?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>24
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H28
<400>24
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Leu?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>25
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H29
<221>CDS
<222>(1)...(1074)
<400>25
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>26
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H29
<400>26
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>27
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H30
<221>CDS
<222>(1)...(1074)
<400>27
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?ttc?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Phe?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?agc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>28
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H30
<400>28
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Phe?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>29
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H3l
<221>CDS
<222>(1)...(1074)
<400>29
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?lle?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gag?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttc?gca?tac?ggc?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tcc?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?cag?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aac?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gat?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>30
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H31
<400>30
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>31
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H32
<221>CDS
<222>(1)...(1074)
<400>31
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttc?gca?tac?ggc?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?caa?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggg?aag?gtc?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gat?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>32
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H32
<400>32
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>33
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H33
<221>CDS
<222>(1)...(1074)
<400>33
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>34
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H33
<400>34
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>35
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H34
<221>CDS
<222>(1)...(1074)
<400>35
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttc?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggc?ctt?cga?ccc 528
Gly?ValAla?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tat?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>36
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H34
<400>36
Met?Leu?His?lle?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?lle?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>37
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H35
<221>CDS
<222>(1)...(1074)
<400>37
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?tcc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Ser?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aat?ttc?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Phe?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?gcg?cac?gag?gcc?tat?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Tyr?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ctg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tca?tgg?agt?cgc?cac?ggg?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Ser?Arg?His?Gly?Arg?Met
260 265 270
gag?cac?ctc?ctc?tcc?ggg?ttc?gac?ggc?acc?aaa?gag?gca?ttt?gcc?aag 864
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Glu?Ala?Phe?Ala?Lys
275 280 285
ttg?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?agc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>38
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H35
<400>38
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Ser?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Phe?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Tyr?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Ser?Arg?His?Gly?Arg?Met
260 265 270
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Glu?Ala?Phe?Ala?Lys
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>39
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H36
<221>CDS
<222>(1)...(1074)
<400>39
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gag?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
atg?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cat?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?att?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?aac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asn?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>40
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H36
<400>40
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asn?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>41
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H37
<221>CDS
<222>(1)...(1074)
<400>41
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?act?gcg?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Thr?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggc?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?ctt?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?gag?gcc?tat?gaa?aag?atc?gtg?tcg?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?acc?gct?ttt?atc?atg?gtg?ggc?aat?gcg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Thr?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?tcc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?acc?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>42
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H37
<400>42
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Thr?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Thr?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>43
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H38
<221>CDS
<222>(1)...(1074)
<400>43
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?tgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gtc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?tgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Cys?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gaa?gct?gga?atc?atc?ggc?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Ash?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctc?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gaa?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?acg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Thr?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>44
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H38
<400>44
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Cys?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Thr?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>45
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H40
<221>CDS
<222>(1)...(1074)
<400>45
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
atg?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cat?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?att?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>46
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H40
<400>46
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>47
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H41
<221>CDS
<222>(1)...(1047)
<400>47
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggc?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?ctt?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?gag?gcc?tat?gaa?aag?atc?gtg?tcg?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?gcg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?tcc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?acc?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?aggtctgcgc 1057
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu
340 345
tcaacgccag?gctttga 1074
<210>48
<211>349
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H41
<400>48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?ValArg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu
340 345
<210>49
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H42
<221>CDS
<222>(1)...(1074)
<400>49
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?tgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gtc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?ValTyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
atg?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggg?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cat?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?att?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?caa?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?ttg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?ggc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aat?gcc?aag?ctt?tga 1074
Leu?Asn?Ala?Lys?Leu *
355
<210>50
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H42
<400>50
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Ile?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Leu?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Gly?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Lys?Leu
355
<210>51
<211>1074
<212>DNA
＜213〉artificial sequence
<220>
<223>H43
<221>CDS
<222>(1)...(1074)
<400>51
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aga 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
tac?tac?gag?gag?att?tgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
agg?gca?gcc?cgt?gat?ggc?tgc?acc?act?gtc?tat?tgt?caa?ctt?cca?aat 336
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggc?gtc?gcc?gtc?aac?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?ctt?cct?tcg?cat?ctt?gcc?ctc?cgc?atg?gcg?ctc?gaa?agc?act 576
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?gag?gcc?tat?gaa?aag?atc?gtg?tcg?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?gcg?cac?gag?gca?ttc?ggt?ctg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttt?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?ccc?cac?ggc?gaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
aaa?gag?atc?gat?ccc?tta?ccg?gac?tcg?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?cac?ctc?ctc?tcc?ggg?ttc?gac?ggt?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gag?gac?gaa?gac?aat?tat?ccc?ttt?ggc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gta?ggc?aag?agc?aga?ggc?gcg?acc?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgt?ttt?gac?gag?gag?gat?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>52
<211>357
<212>PRT
＜213〉artificial sequence
<220>
＜223〉acyltransferase H43
<400>52
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Arg?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Pro?His?Gly?Glu?Asn?Glu
245 250 255
Lys?Glu?Ile?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Gly?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Val?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>53
<211>1074
<212>DNA
<213>Penicillium?chrysogenum
<220>
<221>CDS
<222>(1)...(1074)
<400>53
atg?ctt?cac?atc?ctc?tgt?caa?ggc?act?ccc?ttt?gaa?atc?ggc?tac?gaa 48
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?gcc?gtg?ata?gcc?aga?agc?att?gac?ttc?gcc 96
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
gtc?gat?ctc?atc?cga?ggg?aaa?acg?aag?aag?acg?gac?gaa?gag?ctt?aaa 144
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
cag?gta?ctc?tcg?caa?ctg?ggg?cgc?gtg?atc?gag?gaa?aga?tgg?ccc?aaa 192
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
tac?tac?gag?gag?att?cgc?ggt?att?gca?aag?ggc?gct?gaa?cgc?gat?gtc 240
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
tcc?gag?att?gtc?atg?ctt?aat?acc?cgc?acg?gaa?ttt?gca?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
aag?gca?gcc?cgt?gat?ggc?tgc?acc?act?gcc?tat?tgt?caa?ctt?cca?aat 336
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
gga?gcc?ctc?cag?ggc?caa?aac?tgg?gat?ttc?ttt?tct?gcc?acc?aaa?gag 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
aac?ctg?atc?cgg?tta?acg?atc?cgt?cag?gcc?gga?ctt?ccc?acc?atc?aaa 432
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
ttc?ata?acc?gag?gct?gga?atc?atc?ggg?aag?gtt?gga?ttt?aac?agt?gcg 480
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggc?gtc?gcc?gtc?aat?tac?aac?gcc?ctt?cac?ctt?cag?ggt?ctt?cga?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
acc?gga?gtt?cct?tcg?cat?att?gcc?ctc?cgc?ata?gcg?ctc?gaa?agc?act 576
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?cct?tcc?cag?gcc?tat?gac?cgg?atc?gtg?gag?caa?ggc?gga?atg?gcc 624
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
gcc?agc?gct?ttt?atc?atg?gtg?ggc?aat?ggg?cac?gag?gca?ttt?ggt?ttg 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
gaa?ttc?tcc?ccc?acc?agc?atc?cga?aag?cag?gtg?ctc?gac?gcg?aat?ggt 720
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
agg?atg?gtg?cac?acc?aac?cac?tgc?ttg?ctt?cag?cac?ggc?aaa?aat?gag 768
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
aaa?gag?ctc?gat?ccc?tta?ccg?gac?tca?tgg?aat?cgc?cac?cag?cgt?atg 816
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
gag?ttc?ctc?ctc?gac?ggg?ttc?gac?ggc?acc?aaa?cag?gca?ttt?gcc?cag 864
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
ctc?tgg?gcc?gac?gaa?gac?aat?tat?ccc?ttt?agc?atc?tgc?cgc?gct?tac 912
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
gag?gag?ggc?aag?agc?aga?ggc?gcg?act?ctg?ttc?aat?atc?atc?tac?gac 960
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
cat?gcc?cgt?aga?gag?gca?acg?gtg?cgg?ctt?ggc?cgg?ccg?acc?aac?cct 1008
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
gat?gag?atg?ttt?gtc?atg?cgg?ttt?gac?gag?gag?gac?gag?agg?tct?gcg 1056
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
ctc?aac?gcc?agg?ctt?tga 1074
Leu?Asn?Ala?Arg?Leu *
355
<210>54
<211>357
<212>PRT
＜213〉Penicillium chrysogenum acyltransferase
<400>54
Met?Leu?His?Ile?Leu?Cys?Gln?Gly?Thr?Pro?Phe?Glu?Ile?Gly?Tyr?Glu
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Ala?Val?Ile?Ala?Arg?Ser?Ile?Asp?Phe?Ala
20 25 30
Val?Asp?Leu?Ile?Arg?Gly?Lys?Thr?Lys?Lys?Thr?Asp?Glu?Glu?Leu?Lys
35 40 45
Gln?Val?Leu?Ser?Gln?Leu?Gly?Arg?Val?Ile?Glu?Glu?Arg?Trp?Pro?Lys
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Arg?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Asp?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Lys?Ala?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Ala?Tyr?Cys?Gln?Leu?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Ser?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Arg?Leu?Thr?Ile?Arg?Gln?Ala?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Phe?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?Gln?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Val?Pro?Ser?His?Ile?Ala?Leu?Arg?Ile?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Gln?Ala?Tyr?Asp?Arg?Ile?Val?Glu?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Gly?His?Glu?Ala?Phe?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Thr?Ser?Ile?Arg?Lys?Gln?Val?Leu?Asp?Ala?Asn?Gly
225 230 235 240
Arg?Met?Val?His?Thr?Asn?His?Cys?Leu?Leu?Gln?His?Gly?Lys?Asn?Glu
245 250 255
Lys?Glu?Leu?Asp?Pro?Leu?Pro?Asp?Ser?Trp?Asn?Arg?His?Gln?Arg?Met
260 265 270
Glu?Phe?Leu?Leu?Asp?Gly?Phe?Asp?Gly?Thr?Lys?Gln?Ala?Phe?Ala?Gln
275 280 285
Leu?Trp?Ala?Asp?Glu?Asp?Asn?Tyr?Pro?Phe?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Glu?Glu?Gly?Lys?Ser?Arg?Gly?Ala?Thr?Leu?Phe?Asn?Ile?Ile?Tyr?Asp
305 310 315 320
His?Ala?Arg?Arg?Glu?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Thr?Asn?Pro
325 330 335
Asp?Glu?Met?Phe?Val?Met?Arg?Phe?Asp?Glu?Glu?Asp?Glu?Arg?Ser?Ala
340 345 350
Leu?Asn?Ala?Arg?Leu
355
<210>55
<211>1074
<212>DNA
<213>Aspergillus?nidulans
<220>
<221>CDS
<222>(1)...(1074)
<400>55
atg?ctt?cac?gta?act?tgc?caa?ggt?acc?ccc?tcc?gaa?atc?ggc?tat?cac 48
Met?Leu?His?Val?Thr?Cys?Gln?Gly?Thr?Pro?Ser?Glu?Ile?Gly?Tyr?His
1 5 10 15
cat?ggc?tct?gct?gcc?aaa?ggc?gag?att?gcg?aaa?gcc?att?gac?ttc?gca 96
His?Gly?Ser?Ala?Ala?Lys?Gly?Glu?Ile?Ala?Lys?Ala?Ile?Asp?Phe?Ala
20 25 30
act?ggc?ctc?att?cat?ggc?aaa?aca?aaa?aag?aca?cag?gcg?gag?ctt?gaa 144
Thr?Gly?Leu?Ile?His?Gly?Lys?Thr?Lys?Lys?Thr?Gln?Ala?Glu?Leu?Glu
35 40 45
cag?ctc?ctc?agg?gag?ttg?gag?cag?gtg?atg?aaa?cag?cgc?tgg?ccg?aga 192
Gln?Leu?Leu?Arg?Glu?Leu?Glu?Gln?Val?Met?Lys?Gln?Arg?Trp?Pro?Arg
50 55 60
tac?tat?gag?gaa?atc?tgc?gga?atc?gca?aag?ggt?gcg?gaa?cgc?gaa?gta 240
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Glu?Val
65 70 75 80
tcg?gag?att?gtc?atg?ctc?aac?act?cgt?acg?gaa?ttc?gcg?tac?ggg?ctc 288
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
gta?gaa?gcc?cgg?gac?ggg?tgc?acc?act?gtt?tac?tgc?aaa?acc?ccc?aat 336
Val?Glu?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Lys?Thr?Pro?Asn
100 105 110
gga?gcg?cta?cag?ggc?cag?aac?tgg?gac?ttc?ttc?acc?gca?acc?aaa?gaa 384
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Thr?Ala?Thr?Lys?Glu
115 120 125
aac?ttg?atc?cag?tta?aca?att?tgt?cag?ccg?ggt?cta?ccc?act?atc?aaa 432
Asn?Leu?Ile?Gln?Leu?Thr?Ile?Cys?Gln?Pro?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
atg?att?aca?gaa?gct?ggt?atc?att?ggc?aaa?gtg?ggt?ttc?aac?agt?gct 480
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
ggt?gtc?gct?gtc?aat?tac?aat?gca?cta?cac?cta?cat?ggc?ctc?cgt?ccc 528
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
act?ggc?ctc?ccc?tcg?cat?ctc?gcg?ctg?cgc?atg?gcc?ctc?gaa?agt?aca 576
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
tct?ccg?tct?gag?gcg?tat?gaa?aaa?atc?gtc?tcg?caa?ggg?ggc?atg?gcg 624
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
gct?agc?gcg?ttc?atc?atg?gtg?ggc?aac?gca?cac?gag?gcc?tac?ggg?cta 672
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Tyr?Gly?Leu
210 215 220
gag?ttc?tcg?ccc?atc?agc?ttg?tgc?aag?caa?gtt?gct?gac?acc?aat?ggg 720
Glu?Phe?Ser?Pro?Ile?Ser?Leu?Cys?Lys?Gln?Val?Ala?Asp?Thr?Asn?Gly
225 230 235 240
cgg?ata?gtg?cat?acg?aac?cac?tgc?ctc?ctc?aac?cat?ggg?cca?tcg?gcg 768
Arg?Ile?Val?His?Thr?Asn?His?Cys?Leu?Leu?Asn?His?Gly?Pro?Ser?Ala
245 250 255
caa?gag?ctt?aat?ccc?ctg?ccg?gac?tcg?tgg?agc?cgc?cac?ggg?cgg?atg 816
Gln?Glu?Leu?Asn?Pro?Leu?Pro?Asp?Ser?Trp?Ser?Arg?His?Gly?Arg?Met
260 265 270
gaa?cat?ctc?ctc?tct?ggt?ttt?gac?ggc?acg?aag?gag?gca?ttt?gcg?aag 864
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Glu?Ala?Phe?Ala?Lys
275 280 285
ttg?tgg?gag?gac?gaa?gac?aac?tac?cct?ctc?tcg?atc?tgc?cgg?gca?tat 912
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Leu?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
aag?gaa?ggg?aaa?agt?aga?ggc?tcc?act?ctt?ttc?aac?atc?gtc?ttc?gat 960
Lys?Glu?Gly?Lys?Ser?Arg?Gly?Ser?Thr?Leu?Phe?Asn?Ile?Val?Phe?Asp
305 310 315 320
cat?gtg?ggc?cgg?aag?gca?aca?gtg?cgg?ctg?ggc?cgg?ccc?aat?aac?cct 1008
His?Val?Gly?Arg?Lys?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Asn?Asn?Pro
325 330 335
gat?gag?acc?ttt?gtc?atg?acc?ttt?agc?aat?ctg?gat?acc?aag?tcc?gcg 1056
Asp?Glu?Thr?Phe?Val?Met?Thr?Phe?Ser?Asn?Leu?Asp?Thr?Lys?Ser?Ala
340 345 350
atc?caa?gcc?aac?att?tga 1074
Ile?Gln?Ala?Asn?Ile *
355
<210>56
<211>357
<212>PRT
＜213〉Aspergillus nidulans acyltransferase
<400>56
Met?Leu?His?Val?Thr?Cys?Gln?Gly?Thr?Pro?Ser?Glu?Ile?Gly?Tyr?His
1 5 10 15
His?Gly?Ser?Ala?Ala?Lys?Gly?Glu?Ile?Ala?Lys?Ala?Ile?Asp?Phe?Ala
20 25 30
Thr?Gly?Leu?Ile?His?Gly?Lys?Thr?Lys?Lys?Thr?Gln?Ala?Glu?Leu?Glu
35 40 45
Gln?Leu?Leu?Arg?Glu?Leu?Glu?Gln?Val?Met?Lys?Gln?Arg?Trp?Pro?Arg
50 55 60
Tyr?Tyr?Glu?Glu?Ile?Cys?Gly?Ile?Ala?Lys?Gly?Ala?Glu?Arg?Glu?Val
65 70 75 80
Ser?Glu?Ile?Val?Met?Leu?Asn?Thr?Arg?Thr?Glu?Phe?Ala?Tyr?Gly?Leu
85 90 95
Val?Glu?Ala?Arg?Asp?Gly?Cys?Thr?Thr?Val?Tyr?Cys?Lys?Thr?Pro?Asn
100 105 110
Gly?Ala?Leu?Gln?Gly?Gln?Asn?Trp?Asp?Phe?Phe?Thr?Ala?Thr?Lys?Glu
115 120 125
Asn?Leu?Ile?Gln?Leu?Thr?Ile?Cys?Gln?Pro?Gly?Leu?Pro?Thr?Ile?Lys
130 135 140
Met?Ile?Thr?Glu?Ala?Gly?Ile?Ile?Gly?Lys?Val?Gly?Phe?Asn?Ser?Ala
145 150 155 160
Gly?Val?Ala?Val?Asn?Tyr?Asn?Ala?Leu?His?Leu?His?Gly?Leu?Arg?Pro
165 170 175
Thr?Gly?Leu?Pro?Ser?His?Leu?Ala?Leu?Arg?Met?Ala?Leu?Glu?Ser?Thr
180 185 190
Ser?Pro?Ser?Glu?Ala?Tyr?Glu?Lys?Ile?Val?Ser?Gln?Gly?Gly?Met?Ala
195 200 205
Ala?Ser?Ala?Phe?Ile?Met?Val?Gly?Asn?Ala?His?Glu?Ala?Tyr?Gly?Leu
210 215 220
Glu?Phe?Ser?Pro?Ile?Ser?Leu?Cys?Lys?Gln?Val?Ala?Asp?Thr?Asn?Gly
225 230 235 240
Arg?Ile?Val?His?Thr?Asn?His?Cys?Leu?Leu?Asn?His?Gly?Pro?Ser?Ala
245 250 255
Gln?Glu?Leu?Asn?Pro?Leu?Pro?Asp?Ser?Trp?Ser?Arg?His?Gly?Arg?Met
260 265 270
Glu?His?Leu?Leu?Ser?Gly?Phe?Asp?Gly?Thr?Lys?Glu?Ala?Phe?Ala?Lys
275 280 285
Leu?Trp?Glu?Asp?Glu?Asp?Asn?Tyr?Pro?Leu?Ser?Ile?Cys?Arg?Ala?Tyr
290 295 300
Lys?Glu?Gly?Lys?Ser?Arg?Gly?Ser?Thr?Leu?Phe?Asn?Ile?Val?Phe?Asp
305 310 315 320
His?Val?Gly?Arg?Lys?Ala?Thr?Val?Arg?Leu?Gly?Arg?Pro?Asn?Asn?Pro
325 330 335
Asp?Glu?Thr?Phe?Val?Met?Thr?Phe?Ser?Asn?Leu?Asp?Thr?Lys?Ser?Ala
340 345 350
Ile?Gln?Ala?Asn?Ile
355
<210>57
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>57
tctagacata?tgcttcacat?cctctgtcaa?ggcactccc 39
<210>58
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>58
tttgaaatcg?gctacgaaca?tggctctgct?gccaaagcc 39
<210>59
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>59
gtgatagcca?gaagcattga?cttcgccgtc?gatctcatcc?ga 42
<210>60
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>60
gggaaaacga?agaagacgga?cgaagagctt?aaacaggta 39
<210>61
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>61
ctctcgcaac?tggggcgcgt?gatcgaggaa?agatggccc 39
<210>62
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>62
aaatactacg?aggagattcg?cggtattgca?aagggcgctg?aa 42
<210>63
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>63
cgcgatgtct?ccgagattgt?catgcttaat?acccgcacg 39
<210>64
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>64
gaatttgcat?acgggctcaa?ggcagcccgt?gatggctgc 39
<210>65
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>65
accactgcct?attgtcaact?tccaaatgga?gccctccagg?gc?42
<210>66
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>66
caaaactggg?atttcttttc?tgccaccaaa?gagaacctg 39
<210>67
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>67
atccggttaa?cgatccgtca?ggccggactt?cccaccatc 39
<210>68
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>68
aaattcataa?ccgaggctgg?aatcatcggg?aaggttggat?tt 42
<210>69
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>69
aacagtgcgg?gcgtcgccgt?caattacaac?gcccttcac 39
<210>70
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>70
cttcagggtc?ttcgacccac?cggagttcct?tcgcatatt 39
<210>71
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>71
gccctccgca?tagcgctcga?aagcacttct?ccttcccagg?cc 42
<210>72
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>72
tatgaccgga?tcgtggagca?aggcggaatg?gccgccagc 39
<210>73
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>73
gcttttatca?tggtgggcaa?tgggcacgag?gcatttggt 39
<210>74
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>74
ttggaattct?cccccaccag?catccgaaag?caggtgctcg?ac 42
<210>75
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>75
gcgaatggta?ggatggtgca?caccaaccac?tgcttgctt 39
<210>76
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>76
cagcacggca?aaaatgagaa?agagctcgat?cccttaccg 39
<210>77
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>77
gactcatgga?atcgccacca?gcgtatggag?ttcctcctcg?ac 42
<210>78
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>78
gggttcgacg?gcaccaaaca?ggcatttgcc?cagctctgg 39
<210>79
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>79
gccgacgaag?acaattatcc?ctttagcatc?tgccgcgct 39
<210>80
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>80
tacgaggagg?gcaagagcag?aggcgcgact?ctgttcaata?tc 42
<210>81
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>81
atctacgacc?atgcccgtag?agaggcaacg?gtgcggctt 39
<210>82
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>82
ggccggccga?ccaaccctga?tgagatgttt?gtcatgcgg 39
<210>83
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>83
tttgacgagg?aggacgagag?gtctgcgctc?aacgccaggc?tt 42
<210>84
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>84
atgttcgtag?ccgatttcaa?agggagtgcc?ttgacagagg?at 42
<210>85
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>85
gtcaatgctt?ctggctatca?cggctttggc?agcagagcc 39
<210>86
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>86
cgtcttcttc?gttttccctc?ggatgagatc?gacggcgaa 39
<210>87
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>87
cacgcgcccc?agttgcgaga?gtacctgttt?aagctcttcg?tc 42
<210>88
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>88
gcgaatctcc?tcgtagtatt?tgggccatct?ttcctcgat 39
<210>89
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>89
aatctcggag?acatcgcgtt?cagcgccctt?tgcaatacc 39
<210>90
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>90
cttgagcccg?tatgcaaatt?ccgtgcgggt?attaagcatg?ac 42
<210>91
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>91
aagttgacaa?taggcagtgg?tgcagccatc?acgggctgc 39
<210>92
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>92
aaagaaatcc?cagttttggc?cctggagggc?tccatttgg 39
<210>93
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>93
ctgacggatc?gttaaccgga?tcaggttctc?tttggtggca?ga 42
<210>94
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>94
tccagcctcg?gttatgaatt?tgatggtggg?aagtccggc 39
<210>95
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>95
ggcgacgccc?gcactgttaa?atccaacctt?cccgatgat 39
<210>96
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>96
ggtgggtcga?agaccctgaa?ggtgaagggc?gttgtaattg?ac 42
<210>97
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>97
ttcgagcgct?atgcggaggg?caatatgcga?aggaactcc 39
<210>98
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>98
ctccacgatc?cggtcatagg?cctgggaagg?agaagtgct 39
<210>99
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>99
attgcccacc?atgataaaag?cgctggcggc?cattccgcct?tg 42
<210>100
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>100
gctggtgggg?gagaattcca?aaccaaatgc?ctcgtgccc 39
<210>101
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>101
caccatccta?ccattcgcgt?cgagcacctg?ctttcggat 39
<210>102
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>102
tttctcattt?ttgccgtgct?gaagcaagca?gtggttggtg?tg?42
<210>103
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>103
ctggtggcga?ttccatgagt?ccggtaaggg?atcgagctc 39
<210>104
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>104
tttggtgccg?tcgaacccgt?cgaggaggaa?ctccatacg 39
<210>105
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>105
gggataattg?tcttcgtcgg?cccagagctg?ggcaaatgcc?tg 42
<210>106
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>106
tctgctcttg?ccctcctcgt?aagcgcggca?gatgctaaa 39
<210>107
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>107
acgggcatgg?tcgtagatga?tattgaacag?agtcgcgcc 39
<210>108
<211>42
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>108
atcagggttg?gtcggccggc?caagccgcac?cgttgcctct?ct 42
<210>109
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>109
cctctcgtcc?tcctcgtcaa?accgcatgac?aaacatctc 39
<210>110
<211>39
<212>DNA
＜213〉artificial sequence
<220>
＜223〉synthetic oligonucleotide
<400>110
cgtcatgaag?agccttcaaa?gcctggcgtt?gagcgcaga 39

Claims (21)

1. have the isolated polypeptide of acyltransferase activity, it is selected from:
(a) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:38 at least 96%;
(b) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:42 at least 95%;
(c) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:52 at least 95%;
(d) has the polypeptide that the aminoacid sequence identical with SEQ ID NO:54 at least 96% and having is selected from least one residue of the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes; With
(e) by the polypeptide of following polynucleotide encoding, described polynucleotide can be hybridized the described nucleotide sequence coded aminoacid sequence that is selected from SEQ ID NO:38 and SEQ ID NO:42 and SEQ ID NO:52 with the whole substantially length of following nucleotide sequence under stringent condition.
2. isolated polypeptide as claimed in claim 1, wherein said isolated polypeptide have the aminoacid sequence identical with SEQ ID NO:38 at least 96%.
3. isolated polypeptide as claimed in claim 1, wherein said isolated polypeptide have the aminoacid sequence identical with SEQ ID NO:42 at least 95%.
4. isolated polypeptide as claimed in claim 1, wherein said isolated polypeptide have the aminoacid sequence identical with SEQ ID NO:52 at least 95%.
5. isolated polypeptide as claimed in claim 1, wherein said isolated polypeptide have the aminoacid sequence identical with SEQ ID NO:54 at least 96% and have at least one residue that is selected from the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes.
6. isolated polypeptide as claimed in claim 5, wherein said aminoacid sequence comprise the 97th arginine.
7. isolated polypeptide as claimed in claim 5, wherein said aminoacid sequence comprise the 221st Xie Ansuan.
8. isolated polypeptide as claimed in claim 5, wherein said aminoacid sequence comprise at least two residues that are selected from the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes.
9. isolated polypeptide as claimed in claim 5, wherein said aminoacid sequence comprise at least four residues that are selected from the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes.
10. isolated polypeptide as claimed in claim 5, wherein said aminoacid sequence comprise at least one residue that is selected from by the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th 's glycine.
11. isolated polypeptide as claimed in claim 1, the acyltransferase activity that wherein said isolated polypeptide has are at least 1.5 times corresponding to the wild-type P.chrysogenum acyltransferase activity of SEQ ID NO:54.
12. isolated polypeptide as claimed in claim 8, the acyltransferase activity that wherein said isolated polypeptide has are at least 2 times corresponding to the wild-type P.chrysogenum acyltransferase activity of SEQ ID NO:54.
13. isolated polypeptide as claimed in claim 8, the acyltransferase activity that wherein said isolated polypeptide has are at least 5 times corresponding to the wild-type P.chrysogenum acyltransferase activity of SEQ ID NO:54.
14. comprise the segmental isolated polypeptide of isopenicillin-n acyltransferase polypeptides, wherein said isopenicillin-n acyltransferase polypeptides is selected from:
(a) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:38 at least 96%;
(b) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:42 at least 95%;
(c) has the polypeptide of the aminoacid sequence identical with SEQ ID NO:52 at least 95%;
(d) has the polypeptide that the aminoacid sequence identical with SEQ ID NO:54 at least 96% and having is selected from least one residue of the group that the glycine by the 97th arginine, the 221st Xie Ansuan, the 251st proline(Pro), the 254th L-glutamic acid, the 259th Isoleucine, the 277th glycine, the 291st L-glutamic acid and the 299th constitutes; With
(e) by the polypeptide of following polynucleotide encoding, described polynucleotide can be hybridized the described nucleotide sequence coded aminoacid sequence that is selected from SEQ ID NO:38 and SEQ ID NO:42 and SEQ ID NO:52 with the whole substantially length of following nucleotide sequence under stringent condition.
The polynucleotide of polypeptide according to claim 1 15. encode.
16. expression cassette, it comprises the polynucleotide as claimed in claim 15 that are operably connected with promotor.
17. host cell, it is converted expresses polynucleotide as claimed in claim 15.
18. prepare the method for isopenicillin-n acyltransferase polypeptides according to claim 1; described method is included in and cultivates the host cell that contains nucleic acid construct under the condition that helps to produce this polypeptide; and randomly reclaiming polypeptide, described nucleic acid construct comprises the nucleotide sequence of this polypeptide of encoding.
19. the method for manufacturing purpose 'beta '-lactam compounds; described method is included in and cultivates the host cell that contains nucleic acid construct under the condition that helps to produce 'beta '-lactam compounds; and; reclaim 'beta '-lactam compounds, described nucleic acid construct comprises the nucleotide sequence of the isopenicillin-n acyltransferase polypeptides as claimed in claim 1 of encoding.
20. method as claimed in claim 19, it also comprises 'beta '-lactam compounds is carried out the N-deacylation, and reclaims the 'beta '-lactam compounds through the N-deacylation.
21. method as claimed in claim 19, wherein the purpose lactam compound is adipyl-7-ADCA.
CNA2006800061244A 2005-02-24 2006-02-24 Altered isopenicillin-n acyltransferase polypeptides and related polynucleotides Pending CN101142312A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05101423.1 2005-02-24
EP05101423 2005-02-24

Publications (1)

Publication Number Publication Date
CN101142312A true CN101142312A (en) 2008-03-12

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Country Status (5)

Country Link
EP (1) EP1851309A2 (en)
KR (1) KR20070107715A (en)
CN (1) CN101142312A (en)
BR (1) BRPI0609248A2 (en)
WO (1) WO2006089946A2 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2054916T3 (en) * 1988-04-08 1994-08-16 Biochemie Gmbh GEN AND GEN PRODUCT FOR THE OBTAINING OF BETA-LACTAMA COMPOUNDS.
FI104984B (en) * 1988-08-11 2000-05-15 Dsm Nv Method for Identifying and Using Biosynthetic or Regulatory Genes to Improve Secondary Metabolite Production

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BRPI0609248A2 (en) 2010-03-09
EP1851309A2 (en) 2007-11-07
WO2006089946A3 (en) 2007-06-21
WO2006089946A2 (en) 2006-08-31
KR20070107715A (en) 2007-11-07

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