KR20070074786A - Functional cosmetic composition comprising oryzae extract - Google Patents

Abstract

A functional cosmetic composition comprising the extract of embryo-retaining polished rice is provided to improve skin wrinkles, whiten the skin and enhance moisturizing effect and elasticity of the skin by promoting growth of fibroblasts and increasing water content in the skin. The functional cosmetic composition comprises 0.01-10 wt.% of the extract of embryo-retaining polished rice which contains antioxidative vitamins including retinol, lutein, tocopherol, lysine, riboflavin, thiamine, phytosterol and GABA(gamma amino butyric acid), palmic acid and oleic acid.

Description

Cosmetic composition containing giant embryonic extract {Functional Cosmetic Composition Comprising oryzae Extract}

Figure 1 is a photograph showing the general and giant embryonic rice.

2 is a graph of fibroblast growth.

3 is a graph of melanin biosynthesis inhibition.

The present invention relates to a cosmetic composition containing a giant embryonic extract, and more particularly, on the basis of the growth and whitening effect of fibroblasts, the skin moisture content increasing ability, and excellent in wrinkle improvement and whitening effect and moisturizing and elasticity improvement of skin. It relates to a cosmetic composition containing a giant embryonic extract.

In general, the wrinkles of the skin can be said to be the form and structure of the skin that is locally formed and settled on the site that is chronically deformed by muscle movement. Examining the mechanism of wrinkle formation with changes can be said to be important for understanding the actual mechanism of wrinkle formation.

In this regard, as skin internal structure changes due to photoaging, epidermis, thickening of the dermis, accumulation of abnormal elastic material, or three-dimensional structure change of elastic fibers have been reported. In addition, for collagen, a major component of skin structure, microfibrillation of fibrous collagen fibers and weakening of fibrous form have been revealed (Zheng P. and kligman LH, uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uvB -induced damage., J. Invest.dermatol., 100,194-199 (1993)).

However, the collagen fiber bundles formed by these bundles are known to have a great influence on the toughness of the skin through the study of the wound healing process, but the effects on the structural change, the physical properties of the skin, and the wrinkle formation by photoaging Not enough reviews have been made so far.

The interest of skin care is increasing, and the signs of blemishes and fine lines, which are often seen at 25 years old, are gradually becoming more aging at the age of 35 years. After that, large wrinkles begin to appear, and by the age of about 55, skin movement is generally reduced. The causes of aging are known to be due to a decrease in moisture retention ability, sebum secretion ability, accumulation of harmful substances into the skin by drying, ultraviolet rays, oxidation, etc., degradation of skin cell movement occurring inside the skin. Some skin care methods that can cope with these changes include improving moisture retention, preventing the removal and generation of harmful substances in the skin, and improving skin cell movement to the best. In addition, as an area of interest, research has been rapidly conducted on the effect of ultraviolet rays on the skin. Sunlight is convinced that it is a detrimental effect of aging, blemishes, pigmentation such as freckles, and longing for white skin has been considered a big challenge from the past.

On the other hand, the well-being boom has recently increased the preference for germinated brown rice over conventional rice. Such germinated brown rice is known to improve the body's immunity and resistance. Germination brown rice is known to be good for the body because all diseases occur when the balance of immunity and resistance of the human body is broken.

Currently, many products related to wrinkle improvement, whitening, and moisturizing and elasticity of the skin have been released, and a lot of researches are being conducted to improve it, but it is difficult to expect much effect on wrinkle improvement, whitening, and moisturizing and elasticity of the skin. There is this.

The present invention has been made to solve the above problems, based on the growth and whitening effect of the fibroblasts, skin moisture content increase ability to improve wrinkles and whitening effect and to improve the moisturizing and elasticity of the skin giant embryo extract It is to provide a cosmetic composition containing.

Cosmetic composition containing the giant embryonic extract according to the present invention for achieving the above object is characterized in that, as a cosmetic composition, the giant embryo germination extract as an active ingredient.

Preferably the giant germ germination extract is characterized in that it contains 0.01 to 10% by weight based on the total weight of the cosmetic composition.

More preferably, the giant embryonic rice is characterized in that at least one or more of giant embryonic brown rice, giant embryonic general rice, giant embryonic germinated general rice, giant embryonic germinated brown rice.

More preferably, the extract of the giant embryo is an extract containing antioxidant vitamins such as retinol, lutein, tocopherol, lysine, riboflavin, thiamine, phytosterol and GABA, palmic acid and oleic acid, which are detected as main components. It is done.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented by way of example only to more specifically describe the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

The present invention takes the active ingredient obtained by extracting the embryo from the large embryo that has been developed as a new breed in conventional cosmetics, and then contained in the cosmetic composition to improve the moisturizing effect, elasticity and whitening effect It relates to a composition.

Rice embryos contain higher quality proteins, vitamins and essential fatty acids than any other nutrients, and are a significant source of bioactive substances. The embryo was extracted from a large variety of rice varieties, and the extract was used as a composition for functional cosmetics. For reference, in Figure 1 "A" is a picture of the general beauty and "B" is a picture of the giant embryo. The function of the extract by germination of giant embryos has high antioxidant activity such as tocopherol and oryzanol, and has abundant amounts of amino acids.

The present invention finds that the extract obtained after extracting the embryos from Hwacheong giant embryos, which have been bred and developed as a new breed of conventional rice varieties Hwacheong rice and Nampoong rice, is effective in improving wrinkles and improving moisturizing elasticity and whitening. The present invention has been completed. As the germination of plant seeds progresses, the physiological activity is increased and its components change. The activity of proteins, amino acids, fatty acids, carbohydrates, minerals, vitamins and various enzymes, phytic acid and gamma aminobutyric acid are increased. As the essential constituents and physiologically active components increased, the activity of the phenolic complex was increased, and the antioxidant activity was increased.Antioxidant activity prevents wrinkles by blocking oxidation, which is one of the main mechanisms of aging and whitening. Whitening efficacy will be obtained. In order to apply the substance having such a control function to a functional cosmetics, the present invention has been completed.

Example 1

Hwacheong giant embryo was provided by Seoul National University Nongsaengdae and Shinji (SeoNong No. 8, varieties protection application; June 2003, 102003000086) After sterilization with benzoate hydrate, immersed in distilled water at 15 ℃ 48 hours, then 27 ℃ Germinated for 3 days, the chaff skin was removed, crushed, and then stored at room temperature with 5 times the amount of solvent (70% water + 30% butylene glycol) for 24 hours at room temperature, and then filtered with a 0.45㎛ filter to extract the large germ germination extract. Got it.

Production Example 1-6 and Comparative Example 1

According to the composition shown in Table 1 below using a composition commonly used in the same industry, the effective extracting ingredients of Example 1 are mixed with oily and aqueous components and surfactants, stirred with a homomixer, heated and cooled, and uniformly mixed with cosmetics. Phosphorus Preparation Example 1-6 and Comparative Example 1 were prepared.

TABLE 1

Raw material name Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 Comparative Example 1 Poly Solate-60 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Stearic acid 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Cerro stearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Seryl Octanoate 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Carbomer 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Triethanolamine 0.2 0.2 0.2 0.2 0.2 0.2 0.2 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Extract of Example 1 0.001 Extract of Example 1 0.01 Extract of Example 1 0.1 Extract of Example 1 One Extract of Example 1 5 Extract of Example 1 10 Extract of Example 1 - antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water Total 100 Total 100 Total 100 Total 100 Total 100 Total 100 Total 100

Experimental Example 1

In this experiment, the content of the components contained in the extract extracted in Example 1 was measured. The contents of gamma aminobutyric acid (hereinafter referred to as "GABA"), phenolic compounds, etc., which occupy the largest amount of embryonic components, were measured. In the case of GABA, the large germ embryo was pulverized with a mortar, and then 5 ml of MeOH was added to inactivate the enzyme. After removal of the lipid fraction by CHCl ₃ treatment, the remaining water-soluble fraction was extracted using GABA using AAG 50 WX (H ⁺ ) resin. Was purified, and the amount of GABA was determined using a GABAase kit (sigma Co.) under the conditions of 0.1M KHPO ₄ (pH 8.6).

In addition, to measure the content of the phenolic compound, 6.5 ml of distilled water and 0.5 ml of Folin-coocalteu's penol reagent (sigma Co.) were added to 0.5 ml of the sample of 1 mg / ml giant germ extract, and left at room temperature for 3 minutes. ₂ CO ₃ was added to a saturated solution and 1ml of deionized water, and then 1.5ml, then was allowed to stand for one hour at room temperature and measure the absorbance of 720nm. The content of phenolic compounds was calculated from the standard curve prepared using gallic acid. The results are shown in Table 2.

TABLE 2

Kinds result GABA content (pm / g seed extract) Germination 13.7 ± 1.01 Giant Embryo Germinated Brown Rice 121.2 ± 10.2 Phenolic Compound Content (µg / mL) Normal germinated brown rice 71.05 Giant Embryo Germinated Brown Rice 229.2

The biggest characteristic of the giant germinated brown rice is that the size of the pear is increased by 322% compared to the existing varieties, and the results of the component analysis show that the content of vitamins, lipids and proteins is increased compared to the conventional rice, and also thiamine In the case of 4 times, alpha-tocopherol (α-tocopherol) was increased by 2 times, riboflabin (riboflabin) was increased by 2 times, lysine (lysine) in the composition of the amino acids also increased. In the case of lipids, palmitic acid and oleic acid increased. In addition, the content of retinol and lutein belong to antioxidant vitamins.

Experimental Example 2

In this experiment, the antioxidant capacity of the extract extracted in Example 1 was measured. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical is a chemically induced radical, which has a characteristic of faint blue indigo color when electron is donated in any reaction system. Therefore, the color difference of the color difference can be measured to determine the electron donating ability of the sample. 1 ml of ethanol, 10 µl of extract of Example 1, and 990 µl of 100 mM disodium acetate buffer (pH 5.5) were added to 0.5 ml of 0.5 mM DPPH solution in a test tube, followed by stirring and inducing reaction for 30 seconds. Measured at The electron donating ability (%) was expressed as [(1-As / Ac) × 100], and the absorbance values of the experimental controls were calculated by substituting As and Ac, respectively, and the results are shown in Table 3.

TABLE 3

extract DPPH clearing Abs. (517 nm) % 1% Ascorbic Acid 0.1111 ± 0.001 88 1% BHT 0.0959 ± 0.001 89.6 Common Brown Germ Extract 0.6182 ± 0.027 33.2 Giant Embryo Germinated Brown Rice Extract 0.2523 ± 0.016 75.8

As can be seen from the results of Table 3, the giant germinated brown rice extract according to the present invention showed an efficacy of about 80% compared to ascorbic acid and BHT, which is known to have a strong antioxidant effect, and its efficacy compared to ordinary brown rice. I could see very good.

[Test Example 1]

This test examined the cell proliferation ability using human skin-derived fibroblasts. Fibroblasts were used by proliferating cells stored in -70 ° C or liquid nitrogen. Fibroblasts cultured in T-75 flasks were washed 2-3 times with PBS, and the adherent cells were dropped using trypsin, and then cells were collected by centrifugation (Heraeus, Labofuge 400R) at 1500 rpm for 5 minutes. After centrifugation, the supernatant is discarded and 10 ml of DMEM (10% FBS) is added to the precipitated cells and mixed evenly. Measure the number of cells using a hemocytometer and divide 200 ⁴ ea / well of cells into each well of 96well. When the cells of about 50% of the well area were grown while incubating at 37 ° C. and 5% CO ₂ , the medium was exchanged with fresh DMEM (FBS10%). Cells were incubated at 37 ° C. and 5% CO ₂ for each time period. MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide, Sigma) was dissolved in PBS to 2 mg / ml and stored in the refrigerator. MTT reagent (5 mg) was used in each well. / Ml in PBS) 20µl each, incubate for 2 more hours, discard the supernatant, add 150µL of DMSO and mix well, or mix and add 100µl of 0.04N-HCl. After completion of all steps, measurements were made at 560 nm using an ELISA leader (μQuant, Bio-Tek instrument INC.) (Reference: 650 nm).

The measurement results are shown in Figure 2 and the effect of Example 1 showed a cell proliferation effect of about 85% compared to when the control was seen as 0 after 48 hours in 0.1% treatment.

[Test Example 2]

This test tested skin elasticity effect using Preparation Example 4 and Comparative Example 1. Comparison of Preparation Example 4 and 60 for 60 healthy women (average age 29.5 years old) divided into 6 groups at the temperature of 22-24 ° C and 55% RH, and applied to the actual human body based on the contents of Experimental Example 1 After applying the cosmetic composition of Example 1 for 12 weeks (2 times / day) around the eyes, respectively, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The test results are described in Table 4 below as ΔR5 values (R5 (12 weeks) -R5 (0 weeks)), which are average values for each test group of the Cutometer SEM 575. The R5 value is a value indicating a modulus of elasticity. Closer indicates better elasticity.

In addition, the degree of improvement was calculated by the following equation.

[Equation 1]

TABLE 4

Before application After application Skin elasticity enhancing effect (△ R5) % Improvement Preparation Example 4 0.74 1.16 0.42 56.76 Comparative Example 1 0.71 0.81 0.1 14.08

According to Table 4, when using Preparation Example 4 containing the extract according to Example 1 of the present invention, the skin elasticity increased by 42.68% compared to Comparative Example 1. Therefore, it can be seen that the cosmetic composition containing the extract according to Example 1 according to the present invention enhances skin elasticity more effectively than when using an ingredient of NMF (Natural Moisturizing Factor).

[Test Example 3]

This test measured skin moisture content using Preparation Example 4 and Comparative Example 1. In order to see the improvement of the moisture retention amount on the skin, by using the Preparation Example 4 by measuring the amount of moisture contained in the skin after application, its function was compared and confirmed. Seventy healthy women (mean age 29.5 years) were divided into seven groups of 70 healthy women (22 ~ 24 ℃, 55% relative humidity, no air flow). The cosmetic compositions of Preparation Example 4 and Comparative Example 1 were applied twice a day for 6 weeks to the entire face centered around the eyes in each group. By using Skicon-200 (IBS Co. Japan), the moisture content of the cheek area was measured before and after application to measure the persistence of the moisturizing effect. The results are shown in Table 5 as an average value for each test group.

The improvement was calculated according to the following equation (2).

[Equation 2]

TABLE 5

Before the test 6 weeks after application Improvement (%) Preparation Example 4 630 ± 10 890 ± 10 41.27 Comparative Example 1 630 ± 10 635 ± 10 0.78

According to Table 5, it can be seen that the improvement of the preparation example 4 using 1% by weight of the extract according to Example 1 is much superior to the improvement of Comparative Example 1, which is the result of many experiments in Example 1 in the cosmetic composition It can be seen that it is preferable to contain the extract according to 0.1 to 10% by weight.

[Test Example 4]

This test measured the tyrosinase inhibitory effect of the giant germinated brown rice extract of Example 1 and the whitening raw materials commonly used in cosmetics. The enzyme tyrosinase was extracted from mushrooms, and SIGMA T7755's was used. First, prepare tyrosine, a substrate, in 0.3mg / ml of distilled water, add 1.0ml of this solution to the test tube, add 1.0ml of potassium phosphate buffer solution (0.1M, pH 6.8) and dilute solution for each extract. 0.9ml each was added to the reaction solution and reacted for 10 minutes in a 37 ℃ thermostat. The diluting solvent used ethanol and water in 6: 4, and 0.9 ml of arbutin, a comparative raw material, was used instead of the extract as a control. 0.1 ml of 2,500 unit / m1 tyrosinase solution was added to the reaction solution, and then reacted for 10 minutes in a 37 ° C. thermostatic bath. The test tube containing the reaction solution was placed in ice and quenched to stop the reaction. To determine the tyrosinase inhibition rate of each extract by the following equation (3).

[Equation 3]

The tyrosinase inhibition rate of the identified giant embryonic germinated brown rice extract is 97.67% at the experimental concentration of 1.000%, as shown in Table 6. In Table 6, "ND" represents "Not Detect".

TABLE 6

density Tyrosinase inhibition rate (%) Giant Germination Embryo Extract Arbutin 0.001% 54.25 46.85 0.010% 57.21 64.21 0.100% 78.56 67.24 1.000% 97.67 24.31 2.000% 92.14 30.54 5.000% 87.21 24.18 10.00% 60.11 ND 20.00% 35.12 ND

[Test Example 5]

In this test, the inhibitory effect of the amount of melanin synthesis was tested using the giant germinated brown rice extract based on the results in Test Example 4. Human Melanocytes isolated from human tissues were cultured using primary RPMI 1640 medium at 2 x 10 ⁴ / dish concentration in 60 mm culture dish after primary passage. After confirming that the cells adhered to the dish, the giant germinated brown rice extract was treated by concentration from 0.001% to 10.00%. After treatment with giant embryonic germinated brown rice extract by concentration, the cells were detached with Trypsin-EDTA on the 3rd day, and 2 × 10 ⁵ cells were uniformly distributed. Then, 100 μl of 1N NaOH solution was added and treated at 37 ° C. for 12 hours. Completely lyse the cells. The absorbance of 500 μl of sample in which cells were completely dissolved was measured at 490 nm. As a standard indicator, 10 mg of synthetic melanin (M8601) from Sigma was dissolved in 10 ml of 1N NaOH to prepare a stock solution (1 mg / ml), followed by 700 µg / ml, 300 µg / ml, 100 µg / ml, and 70 µg / ml. Ml, 30 μg / ml, 10 μg / ml, 7 μg / ml, 3 μg / ml, 1 μg / ml, 0.1 μg / ml and 0 μg / ml were prepared and the absorbance was measured at 490 nm.

As a result of the measurement, the amount of melanin synthesis was decreased up to 20% in 0.1% of the embryonic brown germinated brown rice extract, and the results are shown in Table 7 and FIG. 3.

TABLE 7

Giant Germinated Brown Rice Extract Melanin Reduction (%) 0.001% 13.48 0.010% 37.91 0.100% 49.66 1.000% 42.92 2.000% 28.42 5.000% 17.86 10.00% 7.3 20.00% 3.37

[Test Example 6]

In this test example, a test for measuring the whitening effect of the cosmetic compositions according to Production Examples 1 to 5 and Comparative Example 1 was conducted. Set up 2 x 2㎠ areas on the lower arms and arms of 30 healthy male and female subjects, and put aluminum foil around the arm so that the ultraviolet ray is irradiated only on the experimental area, and use UVGL-58 (Upland, USA) lamp at a distance of 10 cm. Irradiation was performed three times in succession once daily with x 10 ⁻⁷ erg / cm 3 / circuit. Before the irradiation, the test site was well washed with 70% alcohol solution, and after irradiation, the preparation Example 1-5 containing the giant germ embryo extract was applied three times a day for 3 weeks. After 3 weeks of application, the degree of pigmentation was determined visually, and the test group evaluated how much the pigmentation was suppressed compared to the control group (Comparative Example 1) in three stages of good effect, normal effect, and no effect. Indicated.

TABLE 8

Production Example Effective usually no effect Preparation Example 1 25 persons 5 people 0 people Preparation Example 2 26 people 4 people 0 people Preparation Example 3 28 people 2 people 0 people Preparation Example 4 28 people 2 people 0 people Preparation Example 5 24 people 6 people 0 people Comparative Example 1 2 people 4 people 24 people

As can be seen in Table 8, in the case of the cosmetic composition containing 0.1% or 1% giant embryonic germinated brown rice extract, it can be seen that the whitening effect of about 93.4% can be seen.

[Test Example 7]

In this test example, in order to confirm that the cosmetic composition containing the extract of Example 1 is free of skin irritation and other side effects, the patch formulation of the human body in the cream formulation of Comparative Example 1 and Preparation Examples 3 and 4 (Patch) test). The test consisted of 30 healthy males and females who placed cosmetics directly in aluminum discs using a Finn chamber on the back or treated them with 40 μL of paper discs and fixed them on the skin with a scanpore tape. After 24 hours, the patch was removed and the result was determined after 4 hours. Results are expressed numerically according to the severity and swelling severity, and the criteria for evaluation are based on the criteria of the International Contact Dermatitis Research Group (ICDRG) (Wooding et al, 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al, 1993).

Criteria

0: no redness

1: erythema

2: redness and papules

3: redness, papules and vesicles

4: severe edema and vesicles

The stimulus degree was calculated according to the following Equation 4 based on the score determined according to the criteria, and the results are shown in Table 9 below.

[Equation 4]

TABLE 9

Preparation Example 3 Preparation Example 4  Comparative Example 1 Irritation degree (%) 0.01 0.02 5.0

As can be seen from the results in Table 9, Preparation Examples 3 and 4 according to the present invention did not have skin irritation or other stimulation, but in Comparative Example 1 it can be seen that there is a skin inflammation and other stimulation.

As described above, the cosmetic composition containing the giant embryonic extract according to the present invention is excellent in improving the antiwrinkle and whitening effect and improving the moisturizing and elasticity of the skin based on the growth and whitening effect of fibroblasts and the increase in skin moisture content. It can be seen that there is an effect, and based on this, it can be seen that it is valuable as a composite functional raw material that can be used as a main raw material of cosmetics.

Claims (4)

As a cosmetic composition, Giant embryonic germination extract, characterized in that as an active ingredient, a cosmetic composition containing a giant embryo extract. The method of claim 1, The giant germ germination extract is characterized in that it contains 0.01 to 10% by weight based on the total weight of the cosmetic composition, cosmetic composition containing a giant embryo extract. The method of claim 2, The giant embryo is characterized in that at least one or more of giant embryonic brown rice, giant embryo general rice, giant embryo germinated general rice, giant embryo germinated brown rice, cosmetic composition containing a giant embryo extract. The method according to any one of claims 1 to 3, The extract of the giant embryo, characterized in that the extract containing the antioxidant vitamins retinol, lutein, tocopherol, lysine, riboflavin, thiamine, phytosterol and GABA, palmic acid, oleic acid detected as the main component Cosmetic composition containing embryonic extract.

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