KR20070062555A - 생물학 및 세포 물질에서 핵산을 분리하는 방법 - Google Patents

생물학 및 세포 물질에서 핵산을 분리하는 방법 Download PDF

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Publication number
KR20070062555A
KR20070062555A KR1020077008601A KR20077008601A KR20070062555A KR 20070062555 A KR20070062555 A KR 20070062555A KR 1020077008601 A KR1020077008601 A KR 1020077008601A KR 20077008601 A KR20077008601 A KR 20077008601A KR 20070062555 A KR20070062555 A KR 20070062555A
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South Korea
Prior art keywords
nucleic acid
solid phase
binding
sample
biological
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Withdrawn
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KR1020077008601A
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English (en)
Korean (ko)
Inventor
하셈 아카반-타프티
Original Assignee
루미젠, 인코포레이티드
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Priority claimed from US10/942,491 external-priority patent/US20050106602A1/en
Application filed by 루미젠, 인코포레이티드 filed Critical 루미젠, 인코포레이티드
Publication of KR20070062555A publication Critical patent/KR20070062555A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/113—Time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020077008601A 2004-09-16 2005-07-05 생물학 및 세포 물질에서 핵산을 분리하는 방법 Withdrawn KR20070062555A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10/942,491 US20050106602A1 (en) 2003-11-17 2004-09-16 Simplified methods for isolating nucleic acids from cellular materials
US10/942,491 2004-09-16
US63862104P 2004-12-22 2004-12-22
US60/638,621 2004-12-22
US11/061,984 2005-02-18
US11/061,984 US20050136477A1 (en) 2003-11-17 2005-02-18 Methods for isolating nucleic acids from biological and cellular materials

Publications (1)

Publication Number Publication Date
KR20070062555A true KR20070062555A (ko) 2007-06-15

Family

ID=36119319

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020077008601A Withdrawn KR20070062555A (ko) 2004-09-16 2005-07-05 생물학 및 세포 물질에서 핵산을 분리하는 방법

Country Status (7)

Country Link
US (1) US20050136477A1 (https=)
EP (1) EP1799846A4 (https=)
JP (1) JP2008513007A (https=)
KR (1) KR20070062555A (https=)
AU (1) AU2005290297A1 (https=)
CA (1) CA2580661A1 (https=)
WO (1) WO2006036243A2 (https=)

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US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
US8552174B2 (en) * 2005-10-31 2013-10-08 Agilent Technologies, Inc. Solutions, methods, and processes for deprotection of polynucleotides
US20070185322A1 (en) * 2006-02-08 2007-08-09 Nexgen Diagnostics Llc Methods of extracting RNA
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US7855281B2 (en) * 2006-03-23 2010-12-21 Agilent Technologies, Inc. Cleavable thiocarbonate linkers for polynucleotide synthesis
JP4986117B2 (ja) * 2006-06-19 2012-07-25 独立行政法人産業技術総合研究所 磁性微粒子に担持したホスホニウム塩とその製造方法、及び該ホスホニウム塩からなる磁性微粒子担持相間移動触媒並びにそれを用いた相間移動反応
WO2008141248A2 (en) 2007-05-10 2008-11-20 Agilent Technologies, Inc. Thiocarbon-protecting groups for rna synthesis
US7790387B2 (en) * 2007-09-24 2010-09-07 Agilent Technologies, Inc. Thiocarbonate linkers for polynucleotides
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
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EP2761001B1 (en) * 2011-09-26 2018-08-01 Qiagen GmbH Rapid method for isolating extracellular nucleic acids
US10072284B2 (en) 2012-06-21 2018-09-11 Monsanto Technology Llc Lysis buffer and methods for extraction of DNA from plant material
EP3102612B1 (en) * 2014-02-07 2024-10-09 European Molecular Biology Laboratory Proteomic sample preparation using paramagnetic beads
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
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JP7476153B2 (ja) * 2021-10-07 2024-04-30 日本電子株式会社 ホスホニウム化合物、誘導体化用試薬キット、質量分析方法、及びホスホニウム化合物の製造方法

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Also Published As

Publication number Publication date
JP2008513007A (ja) 2008-05-01
WO2006036243A8 (en) 2007-12-13
WO2006036243A3 (en) 2007-07-26
WO2006036243A2 (en) 2006-04-06
AU2005290297A1 (en) 2006-04-06
US20050136477A1 (en) 2005-06-23
CA2580661A1 (en) 2006-04-06
EP1799846A2 (en) 2007-06-27
EP1799846A4 (en) 2008-01-16

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Patent event date: 20070416

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PN2301 Change of applicant

Patent event date: 20070904

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

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WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid